LOCUS       DAFFQY010000001        93451 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFFQY010000001 DAFFQY010000000
VERSION     DAFFQY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.001"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            83..970
                     /locus_tag="LRB02_000001"
     CDS             83..970
                     /locus_tag="LRB02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL4658252.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="LRB02_000002"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="LRB02_000002"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL4658253.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="LRB02_000003"
     CDS             2272..2718
                     /locus_tag="LRB02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4658254.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="LRB02_000004"
     CDS             2991..4343
                     /locus_tag="LRB02_000004"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL4658255.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="LRB02_000005"
     CDS             4514..4801
                     /locus_tag="LRB02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658256.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="LRB02_000006"
     CDS             5027..5470
                     /locus_tag="LRB02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4658257.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="LRB02_000007"
     CDS             complement(5476..6660)
                     /locus_tag="LRB02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL4658258.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="LRB02_000008"
     CDS             6928..7626
                     /locus_tag="LRB02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL4658259.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="LRB02_000009"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="LRB02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL4658260.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="LRB02_000010"
     CDS             complement(8436..8969)
                     /locus_tag="LRB02_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658261.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="LRB02_000011"
     CDS             complement(9266..11647)
                     /locus_tag="LRB02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL4658262.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LRB02_000012"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LRB02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL4658263.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="LRB02_000013"
     CDS             12348..12887
                     /locus_tag="LRB02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL4658264.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="LRB02_000014"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="LRB02_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL4658265.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="LRB02_000015"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LRB02_000015"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="LRB02_000016"
     CDS             13994..15160
                     /locus_tag="LRB02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL4658266.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="LRB02_000017"
     CDS             15206..16702
                     /locus_tag="LRB02_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL4658267.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="LRB02_000018"
     CDS             16783..17466
                     /locus_tag="LRB02_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4658268.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="LRB02_000019"
     CDS             18111..19301
                     /locus_tag="LRB02_000019"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL4658269.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="LRB02_000020"
     CDS             19403..20356
                     /locus_tag="LRB02_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL4658270.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="LRB02_000021"
     CDS             20425..21774
                     /locus_tag="LRB02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4658271.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="LRB02_000022"
     CDS             complement(21957..22280)
                     /locus_tag="LRB02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658272.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="LRB02_000023"
     CDS             22409..23572
                     /locus_tag="LRB02_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4658273.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="LRB02_000024"
     CDS             23642..24991
                     /locus_tag="LRB02_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4658274.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="LRB02_000025"
     CDS             complement(25036..25467)
                     /locus_tag="LRB02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4658275.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="LRB02_000026"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="LRB02_000026"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL4658276.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="LRB02_000027"
     CDS             28697..29293
                     /locus_tag="LRB02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4658277.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="LRB02_000028"
     CDS             29415..30563
                     /locus_tag="LRB02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL4658278.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="LRB02_000029"
     CDS             complement(30601..31014)
                     /locus_tag="LRB02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL4658279.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="LRB02_000030"
     CDS             31093..31524
                     /locus_tag="LRB02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL4658280.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="LRB02_000031"
     CDS             complement(31623..31856)
                     /locus_tag="LRB02_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL4658281.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="LRB02_000032"
     CDS             32039..33709
                     /locus_tag="LRB02_000032"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL4658282.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="LRB02_000033"
     CDS             33856..34242
                     /locus_tag="LRB02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658283.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="LRB02_000034"
     CDS             34229..34927
                     /locus_tag="LRB02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4658284.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="LRB02_000035"
     CDS             34932..35396
                     /locus_tag="LRB02_000035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL4658285.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="LRB02_000036"
     CDS             35393..36304
                     /locus_tag="LRB02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4658286.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="LRB02_000037"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="LRB02_000037"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL4658287.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="LRB02_000038"
     CDS             37092..38375
                     /locus_tag="LRB02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL4658288.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="LRB02_000039"
     CDS             38388..39314
                     /locus_tag="LRB02_000039"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL4658289.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="LRB02_000040"
     CDS             39333..40613
                     /locus_tag="LRB02_000040"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL4658290.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="LRB02_000041"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="LRB02_000041"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL4658291.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="LRB02_000042"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="LRB02_000042"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL4658292.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="LRB02_000043"
     CDS             44938..45714
                     /locus_tag="LRB02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL4658293.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="LRB02_000044"
     CDS             45711..46643
                     /locus_tag="LRB02_000044"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL4658294.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="LRB02_000045"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="LRB02_000045"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL4658295.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="LRB02_000046"
     CDS             47363..47992
                     /locus_tag="LRB02_000046"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL4658296.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="LRB02_000047"
     CDS             47980..48294
                     /locus_tag="LRB02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658297.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="LRB02_000048"
     CDS             48332..48973
                     /locus_tag="LRB02_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4658298.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49016..49708
                     /locus_tag="LRB02_000049"
     CDS             49016..49708
                     /locus_tag="LRB02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL4658299.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="LRB02_000050"
     CDS             49705..50607
                     /locus_tag="LRB02_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4658300.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LRB02_000051"
     CDS             complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LRB02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL4658301.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="LRB02_000052"
     CDS             52661..53284
                     /locus_tag="LRB02_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658302.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="LRB02_000053"
     CDS             53734..54783
                     /locus_tag="LRB02_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4658303.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="LRB02_000054"
     CDS             54780..55661
                     /locus_tag="LRB02_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658304.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="LRB02_000055"
     CDS             55664..56434
                     /locus_tag="LRB02_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4658305.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="LRB02_000056"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="LRB02_000056"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL4658306.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="LRB02_000057"
     CDS             57745..58908
                     /locus_tag="LRB02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4658307.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="LRB02_000058"
     CDS             59025..59435
                     /locus_tag="LRB02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4658308.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(59422..59565)
                     /locus_tag="LRB02_000059"
     CDS             complement(59422..59565)
                     /locus_tag="LRB02_000059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658309.1"
                     /translation="MEHIFASGNKKRYRIVEIDTKTSSKQTMIRLLLDVSRKYSSLAV
                     TLG"
     gene            59608..61836
                     /gene="topB"
                     /locus_tag="LRB02_000060"
     CDS             59608..61836
                     /gene="topB"
                     /locus_tag="LRB02_000060"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4658310.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62007..62291
                     /locus_tag="LRB02_000061"
     CDS             62007..62291
                     /locus_tag="LRB02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658311.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62451..62687
                     /locus_tag="LRB02_000062"
     CDS             62451..62687
                     /locus_tag="LRB02_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4658312.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62684..63082
                     /locus_tag="LRB02_000063"
     CDS             62684..63082
                     /locus_tag="LRB02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL4658313.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(63749..64693)
                     /locus_tag="LRB02_000064"
     CDS             complement(63749..64693)
                     /locus_tag="LRB02_000064"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL4658314.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64989..65933)
                     /locus_tag="LRB02_000065"
     CDS             complement(64989..65933)
                     /locus_tag="LRB02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658315.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66154..67368
                     /locus_tag="LRB02_000066"
     CDS             66154..67368
                     /locus_tag="LRB02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4658316.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67390..69405
                     /locus_tag="LRB02_000067"
     CDS             67390..69405
                     /locus_tag="LRB02_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL4658317.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69488..70861)
                     /locus_tag="LRB02_000068"
     CDS             complement(69488..70861)
                     /locus_tag="LRB02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL4658318.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70887..71171)
                     /locus_tag="LRB02_000069"
     CDS             complement(70887..71171)
                     /locus_tag="LRB02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4658319.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71173..73188)
                     /locus_tag="LRB02_000070"
     CDS             complement(71173..73188)
                     /locus_tag="LRB02_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4658320.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73367..73672)
                     /locus_tag="LRB02_000071"
     CDS             complement(73367..73672)
                     /locus_tag="LRB02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL4658321.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73761..74792)
                     /locus_tag="LRB02_000072"
     CDS             complement(73761..74792)
                     /locus_tag="LRB02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4658322.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            74999..75874
                     /locus_tag="LRB02_000073"
     CDS             74999..75874
                     /locus_tag="LRB02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4658323.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76026..76868)
                     /locus_tag="LRB02_000074"
     CDS             complement(76026..76868)
                     /locus_tag="LRB02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4658324.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76960..78837)
                     /locus_tag="LRB02_000075"
     CDS             complement(76960..78837)
                     /locus_tag="LRB02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4658325.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78834..80255)
                     /locus_tag="LRB02_000076"
     CDS             complement(78834..80255)
                     /locus_tag="LRB02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4658326.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80258..83647)
                     /locus_tag="LRB02_000077"
     CDS             complement(80258..83647)
                     /locus_tag="LRB02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL4658327.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83904..85295
                     /locus_tag="LRB02_000078"
     CDS             83904..85295
                     /locus_tag="LRB02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658328.1"
                     /translation="MEKNIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85368..86015)
                     /locus_tag="LRB02_000079"
     CDS             complement(85368..86015)
                     /locus_tag="LRB02_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL4658329.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86023..86709)
                     /locus_tag="LRB02_000080"
     CDS             complement(86023..86709)
                     /locus_tag="LRB02_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658330.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86715..87788)
                     /locus_tag="LRB02_000081"
     CDS             complement(86715..87788)
                     /locus_tag="LRB02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658331.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88010..88684
                     /locus_tag="LRB02_000082"
     CDS             88010..88684
                     /locus_tag="LRB02_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4658332.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88689..90029
                     /locus_tag="LRB02_000083"
     CDS             88689..90029
                     /locus_tag="LRB02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4658333.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90178..91164)
                     /locus_tag="LRB02_000084"
     CDS             complement(90178..91164)
                     /locus_tag="LRB02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL4658334.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91888..92919
                     /locus_tag="LRB02_000085"
     CDS             91888..92919
                     /locus_tag="LRB02_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4658335.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93028..>93451
                     /locus_tag="LRB02_000086"
     CDS             93028..>93451
                     /locus_tag="LRB02_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL4658336.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29895 a        16573 c        18964 g        28019 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagat atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc
    59581 aaaaaattaa gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca
    59641 caagcaaaag cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa
    59701 atcagcgatc cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta
    59761 gatatggttg ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg
    59821 cctattttcc cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg
    59881 atcgtaaaac gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga
    59941 gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag
    60001 aactatttac gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag
    60061 aatctccaac ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac
    60121 gctgattggc tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa
    60181 gggattgatg gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat
    60241 cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta
    60301 attacgacaa acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc
    60361 caagaagagt taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga
    60421 cggattcttg aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta
    60481 tctagtttgc agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat
    60541 gctgctcaag gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac
    60601 attacagaaa atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta
    60661 ggaatagaag aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa
    60721 aaagttcaag aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt
    60781 gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct
    60841 atgtttgctg cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta
    60901 aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc
    60961 aagtcaaaaa agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa
    61021 gtagaactga aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg
    61081 ttgatcacag caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt
    61141 ctcaaagaag tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt
    61201 aagcagaagc attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc
    61261 actctttgta aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc
    61321 tgggaagggt acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag
    61381 aatatcaaaa aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta
    61441 gacttttcag atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat
    61501 caattaggaa gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct
    61561 acatgcttga atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg
    61621 actaaaacga atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt
    61681 tttactggta aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc
    61741 ggtttttctt ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa
    61801 acatcaagaa aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat
    61861 atttattccg aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa
    61921 gcaaaaacgt tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa
    61981 aagtgcattc atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag
    62041 catttctaaa gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag
    62101 catattcacc aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt
    62161 tttttcataa aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg
    62221 tggtagcggc tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat
    62281 attttggtta agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt
    62341 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
    62401 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
    62461 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
    62521 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
    62581 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
    62641 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
    62701 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
    62761 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
    62821 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
    62881 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
    62941 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
    63001 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
    63061 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg
    63121 tcattaagga atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa
    63181 atatggaaaa tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa
    63241 tagtaggaaa tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata
    63301 atatatatta tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc
    63361 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
    63421 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
    63481 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
    63541 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
    63601 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
    63661 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
    63721 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
    63781 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
    63841 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
    63901 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
    63961 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
    64021 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
    64081 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
    64141 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
    64201 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
    64261 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
    64321 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
    64381 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
    64441 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
    64501 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
    64561 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
    64621 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
    64681 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
    64741 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
    64801 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
    64861 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
    64921 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
    64981 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
    65041 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
    65101 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
    65161 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
    65221 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
    65281 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
    65341 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
    65401 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
    65461 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
    65521 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
    65581 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
    65641 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
    65701 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
    65761 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
    65821 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
    65881 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
    65941 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
    66001 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
    66061 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
    66121 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
    66181 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
    66241 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
    66301 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
    66361 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
    66421 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
    66481 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
    66541 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
    66601 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
    66661 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
    66721 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
    66781 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
    66841 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
    66901 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
    66961 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
    67021 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
    67081 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
    67141 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
    67201 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
    67261 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
    67321 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
    67381 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
    67441 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
    67501 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
    67561 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
    67621 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
    67681 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
    67741 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
    67801 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
    67861 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
    67921 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
    67981 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
    68041 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
    68101 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
    68161 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
    68221 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
    68281 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
    68341 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
    68401 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
    68461 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
    68521 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
    68581 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
    68641 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
    68701 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
    68761 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
    68821 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
    68881 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
    68941 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
    69001 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
    69061 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
    69121 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
    69181 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
    69241 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
    69301 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
    69361 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
    69421 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
    69481 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
    69541 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
    69601 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
    69661 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
    69721 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
    69781 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
    69841 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
    69901 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
    69961 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
    70021 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
    70081 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
    70141 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
    70201 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
    70261 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
    70321 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
    70381 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
    70441 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
    70501 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
    70561 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
    70621 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
    70681 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
    70741 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
    70801 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
    70861 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
    70921 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
    70981 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
    71041 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
    71101 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
    71161 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
    71221 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
    71281 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
    71341 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
    71401 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
    71461 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
    71521 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
    71581 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
    71641 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
    71701 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
    71761 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
    71821 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
    71881 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
    71941 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
    72001 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
    72061 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
    72121 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
    72181 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
    72241 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
    72301 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
    72361 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
    72421 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
    72481 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
    72541 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
    72601 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
    72661 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
    72721 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
    72781 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
    72841 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
    72901 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
    72961 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
    73021 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
    73081 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
    73141 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
    73201 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
    73261 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
    73321 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
    73381 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    73441 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    73501 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    73561 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    73621 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    73681 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    73741 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    73801 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    73861 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    73921 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    73981 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    74041 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    74101 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    74161 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    74221 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    74281 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    74341 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    74401 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    74461 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    74521 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    74581 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    74641 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    74701 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    74761 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    74821 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    74881 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    74941 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    75001 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    75061 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    75121 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    75181 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    75241 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    75301 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    75361 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    75421 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    75481 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    75541 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    75601 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    75661 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    75721 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    75781 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    75841 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    75901 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    75961 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    76021 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    76081 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    76141 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    76201 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    76261 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    76321 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    76381 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    76441 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    76501 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    76561 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    76621 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    76681 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    76741 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    76801 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    76861 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    76921 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    76981 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    77041 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    77101 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    77161 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    77221 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    77281 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    77341 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    77401 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    77461 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    77521 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    77581 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    77641 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    77701 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    77761 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    77821 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    77881 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    77941 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    78001 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    78061 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    78121 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    78181 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    78241 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    78301 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    78361 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    78421 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    78481 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    78541 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    78601 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    78661 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    78721 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    78781 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    78841 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    78901 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    78961 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    79021 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    79081 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    79141 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    79201 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    79261 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    79321 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    79381 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    79441 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    79501 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    79561 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    79621 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    79681 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    79741 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    79801 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    79861 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    79921 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    79981 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    80041 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    80101 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    80161 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    80221 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    80281 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    80341 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    80401 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    80461 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    80521 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    80581 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    80641 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    80701 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    80761 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    80821 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    80881 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    80941 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    81001 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    81061 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    81121 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    81181 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    81241 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    81301 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    81361 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    81421 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    81481 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    81541 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    81601 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    81661 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    81721 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    81781 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    81841 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    81901 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    81961 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    82021 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    82081 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    82141 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    82201 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    82261 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    82321 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    82381 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    82441 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    82501 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    82561 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    82621 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    82681 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    82741 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    82801 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    82861 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    82921 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    82981 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    83041 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    83101 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    83161 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    83221 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    83281 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    83341 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    83401 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    83461 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    83521 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    83581 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    83641 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    83701 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    83761 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    83821 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    83881 tgttggaacg aaactataga aagatggaaa aaaacattcg aattctgtcc atttctgaca
    83941 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    84001 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    84061 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    84121 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    84181 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    84241 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    84301 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    84361 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    84421 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    84481 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    84541 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    84601 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    84661 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    84721 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    84781 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    84841 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    84901 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    84961 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    85021 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    85081 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    85141 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    85201 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    85261 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    85321 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    85381 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    85441 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    85501 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    85561 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    85621 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    85681 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    85741 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    85801 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    85861 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    85921 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    85981 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    86041 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    86101 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    86161 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    86221 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    86281 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    86341 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    86401 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    86461 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    86521 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    86581 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    86641 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    86701 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    86761 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    86821 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    86881 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    86941 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    87001 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    87061 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    87121 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    87181 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    87241 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    87301 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    87361 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    87421 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    87481 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    87541 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    87601 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    87661 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    87721 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    87781 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    87841 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    87901 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    87961 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    88021 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    88081 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    88141 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    88201 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    88261 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    88321 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    88381 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    88441 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    88501 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    88561 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    88621 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    88681 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    88741 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    88801 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    88861 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    88921 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    88981 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    89041 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    89101 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    89161 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    89221 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    89281 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    89341 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    89401 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    89461 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    89521 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    89581 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    89641 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    89701 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    89761 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    89821 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    89881 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    89941 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    90001 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    90061 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    90121 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    90181 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    90241 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    90301 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    90361 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    90421 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    90481 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    90541 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    90601 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    90661 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    90721 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    90781 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    90841 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    90901 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    90961 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    91021 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    91081 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    91141 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    91201 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    91261 gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat
    91321 tctaataaaa tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca
    91381 ctcgttgtga tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag
    91441 gattaattct caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag
    91501 taccaactat ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat
    91561 tattgaataa atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata
    91621 aaagaaagag cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac
    91681 cacatagcta aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa
    91741 ataactaaaa atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg
    91801 cttcctaaga tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa
    91861 aatgaacact attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa
    91921 aagttaaagc aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat
    91981 cgttcttccc tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact
    92041 gattatttga gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa
    92101 aaacaagatt atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact
    92161 acagcgaacc aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag
    92221 caatattcat taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag
    92281 atttctcttt tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat
    92341 gcagaacttt tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc
    92401 agagaagcta gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt
    92461 ctgtattgcg gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg
    92521 accaagcaga ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca
    92581 aaagaagcga tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa
    92641 aattcttttg tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa
    92701 attttgagca aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat
    92761 cgttattatg cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat
    92821 cgatacctct catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat
    92881 cacgaattag cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt
    92941 tcacaataaa ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat
    93001 tacttaacaa aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac
    93061 ttatttacga attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat
    93121 gagccacttt cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa
    93181 agtgtgttga aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt
    93241 attatttata agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt
    93301 ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc
    93361 gatcgtttag aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa
    93421 atttcaaatg gaacaaaatc aaaacagatt c
//
LOCUS       DAFFQY010000002        63830 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFFQY010000002 DAFFQY010000000
VERSION     DAFFQY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.002"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            233..475
                     /locus_tag="LRB02_000087"
     CDS             233..475
                     /locus_tag="LRB02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL4658337.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="LRB02_000088"
     CDS             472..942
                     /locus_tag="LRB02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL4658338.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="LRB02_000089"
     CDS             1070..2284
                     /locus_tag="LRB02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL4658339.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3273..3482
                     /locus_tag="LRB02_000090"
     CDS             3273..3482
                     /locus_tag="LRB02_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL4658340.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="LRB02_000091"
     CDS             3594..5207
                     /locus_tag="LRB02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4658341.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="LRB02_000092"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="LRB02_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="LRB02_000093"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="LRB02_000093"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL4658342.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9069
                     /locus_tag="LRB02_000094"
                     /pseudo
     CDS             7610..9069
                     /locus_tag="LRB02_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9290..11053
                     /locus_tag="LRB02_000095"
     CDS             9290..11053
                     /locus_tag="LRB02_000095"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL4658343.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11137..11595
                     /locus_tag="LRB02_000096"
     CDS             11137..11595
                     /locus_tag="LRB02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL4658344.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11704..12387
                     /locus_tag="LRB02_000097"
     CDS             11704..12387
                     /locus_tag="LRB02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL4658345.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12534..13148
                     /locus_tag="LRB02_000098"
     CDS             12534..13148
                     /locus_tag="LRB02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL4658346.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13164..13370
                     /locus_tag="LRB02_000099"
     CDS             13164..13370
                     /locus_tag="LRB02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658347.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQWQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13911..15041
                     /locus_tag="LRB02_000100"
     CDS             13911..15041
                     /locus_tag="LRB02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4658348.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15133..15888
                     /locus_tag="LRB02_000101"
     CDS             15133..15888
                     /locus_tag="LRB02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4658349.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15946..17985
                     /locus_tag="LRB02_000102"
     CDS             15946..17985
                     /locus_tag="LRB02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL4658350.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18094..19245)
                     /locus_tag="LRB02_000103"
     CDS             complement(18094..19245)
                     /locus_tag="LRB02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL4658351.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19399..19929)
                     /locus_tag="LRB02_000104"
     CDS             complement(19399..19929)
                     /locus_tag="LRB02_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL4658352.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSIMPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20028..20378)
                     /locus_tag="LRB02_000105"
     CDS             complement(20028..20378)
                     /locus_tag="LRB02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL4658353.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20535..22064)
                     /gene="ahpF"
                     /locus_tag="LRB02_000106"
     CDS             complement(20535..22064)
                     /gene="ahpF"
                     /locus_tag="LRB02_000106"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL4658354.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22074..22637)
                     /gene="ahpC"
                     /locus_tag="LRB02_000107"
     CDS             complement(22074..22637)
                     /gene="ahpC"
                     /locus_tag="LRB02_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL4658355.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22781..23443)
                     /locus_tag="LRB02_000108"
     CDS             complement(22781..23443)
                     /locus_tag="LRB02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4658356.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23627..25272
                     /locus_tag="LRB02_000109"
                     /pseudo
     CDS             23627..25272
                     /locus_tag="LRB02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            25263..26345
                     /gene="uxuA"
                     /locus_tag="LRB02_000110"
     CDS             25263..26345
                     /gene="uxuA"
                     /locus_tag="LRB02_000110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL4658357.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26453..28006)
                     /locus_tag="LRB02_000111"
                     /pseudo
     CDS             complement(26453..28006)
                     /locus_tag="LRB02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            28154..29295
                     /locus_tag="LRB02_000112"
                     /pseudo
     CDS             28154..29295
                     /locus_tag="LRB02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29421..30500)
                     /locus_tag="LRB02_000113"
     CDS             complement(29421..30500)
                     /locus_tag="LRB02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4658358.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30603..31043)
                     /locus_tag="LRB02_000114"
     CDS             complement(30603..31043)
                     /locus_tag="LRB02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4658359.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31100..31510)
                     /locus_tag="LRB02_000115"
     CDS             complement(31100..31510)
                     /locus_tag="LRB02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL4658360.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31690..32124)
                     /locus_tag="LRB02_000116"
     CDS             complement(31690..32124)
                     /locus_tag="LRB02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4658361.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32224..33072)
                     /locus_tag="LRB02_000117"
     CDS             complement(32224..33072)
                     /locus_tag="LRB02_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4658362.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33461..35227
                     /locus_tag="LRB02_000118"
     CDS             33461..35227
                     /locus_tag="LRB02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL4658363.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35329..36588
                     /locus_tag="LRB02_000119"
     CDS             35329..36588
                     /locus_tag="LRB02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4658364.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36735..38036
                     /locus_tag="LRB02_000120"
     CDS             36735..38036
                     /locus_tag="LRB02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658365.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38039..38890
                     /locus_tag="LRB02_000121"
     CDS             38039..38890
                     /locus_tag="LRB02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658366.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38903..39718
                     /locus_tag="LRB02_000122"
     CDS             38903..39718
                     /locus_tag="LRB02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL4658367.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39869..41035
                     /locus_tag="LRB02_000123"
     CDS             39869..41035
                     /locus_tag="LRB02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4658368.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41265..41912
                     /locus_tag="LRB02_000124"
     CDS             41265..41912
                     /locus_tag="LRB02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL4658369.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41996..42940
                     /locus_tag="LRB02_000125"
     CDS             41996..42940
                     /locus_tag="LRB02_000125"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL4658370.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43075..44527
                     /locus_tag="LRB02_000126"
                     /pseudo
     CDS             43075..44527
                     /locus_tag="LRB02_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            44548..45384
                     /locus_tag="LRB02_000127"
     CDS             44548..45384
                     /locus_tag="LRB02_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL4658371.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45384..46043
                     /locus_tag="LRB02_000128"
     CDS             45384..46043
                     /locus_tag="LRB02_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4658372.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46169..46858
                     /locus_tag="LRB02_000129"
     CDS             46169..46858
                     /locus_tag="LRB02_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658373.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46864..47820
                     /locus_tag="LRB02_000130"
     CDS             46864..47820
                     /locus_tag="LRB02_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL4658374.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47864..48376
                     /locus_tag="LRB02_000131"
     CDS             47864..48376
                     /locus_tag="LRB02_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4658375.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48632..49180)
                     /locus_tag="LRB02_000132"
     CDS             complement(48632..49180)
                     /locus_tag="LRB02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4658376.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49269..50450)
                     /locus_tag="LRB02_000133"
     CDS             complement(49269..50450)
                     /locus_tag="LRB02_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL4658377.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50686..52194)
                     /locus_tag="LRB02_000134"
     CDS             complement(50686..52194)
                     /locus_tag="LRB02_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL4658378.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52353..52544
                     /locus_tag="LRB02_000135"
     CDS             52353..52544
                     /locus_tag="LRB02_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658379.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52747..53664
                     /locus_tag="LRB02_000136"
     CDS             52747..53664
                     /locus_tag="LRB02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL4658380.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53657..54730
                     /locus_tag="LRB02_000137"
     CDS             53657..54730
                     /locus_tag="LRB02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658381.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54878..55396
                     /locus_tag="LRB02_000138"
     CDS             54878..55396
                     /locus_tag="LRB02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658382.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55377..55769
                     /locus_tag="LRB02_000139"
     CDS             55377..55769
                     /locus_tag="LRB02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL4658383.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55845..56780
                     /locus_tag="LRB02_000140"
     CDS             55845..56780
                     /locus_tag="LRB02_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL4658384.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56913..57692
                     /locus_tag="LRB02_000141"
     CDS             56913..57692
                     /locus_tag="LRB02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL4658385.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57685..58002
                     /locus_tag="LRB02_000142"
     CDS             57685..58002
                     /locus_tag="LRB02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL4658386.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58246..59856
                     /locus_tag="LRB02_000143"
     CDS             58246..59856
                     /locus_tag="LRB02_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4658387.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60013..60861
                     /locus_tag="LRB02_000144"
     CDS             60013..60861
                     /locus_tag="LRB02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4658388.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61092..61571
                     /locus_tag="LRB02_000145"
     CDS             61092..61571
                     /locus_tag="LRB02_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658389.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61604..>63830
                     /locus_tag="LRB02_000146"
     CDS             61604..>63830
                     /locus_tag="LRB02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4658390.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT        21423 a        11171 c        12568 g        18668 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 ttttttaaaa gaagatattc ggaagatgaa aagtccttta tcatacgaga aatagagatc
     8221 gcaatcggaa gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa
     8281 acgaagccgt tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac
     8341 ttacaagaag aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat
     8401 gagaacatgg agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat
     8461 gacctatcac tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca
     8521 gaaaatttta ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga
     8581 caagtatttt taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga
     8641 aaaattgtag aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat
     8701 gaaaatttca ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg
     8761 agacaggaaa tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga
     8821 acccaatttc atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt
     8881 tcattagaag aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat
     8941 gctgctagct atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta
     9001 aaaggtgcaa acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat
     9061 atttcttgat gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa
     9121 ggtttttatt tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa
     9181 atttttttaa aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta
     9241 acaaatctat tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt
     9301 attacatgta ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga
     9361 aatgaaacag ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg
     9421 tgggcacgtt ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt
     9481 tgattcacca aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct
     9541 tttaactggt cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc
     9601 caaccaacat gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag
     9661 cttagcaaca ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc
     9721 atttatcggt gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg
     9781 tgttttacaa tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa
     9841 tcatggcagt ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa
     9901 taacctattt aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga
     9961 aacggtgatc cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt
    10021 ccatacggaa aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg
    10081 gcatgtacca tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt
    10141 taatatgatc atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc
    10201 cgcagtcaat gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc
    10261 agatcattat tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt
    10321 ggcttctcag ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata
    10381 tgatcaatta tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg
    10441 cggaacgatc tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc
    10501 aagtatcccg aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct
    10561 agattgggcg ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt
    10621 agaacatggt acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa
    10681 tggaggaaaa gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt
    10741 aaaagctttt agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga
    10801 aaatgacgta gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga
    10861 agatggtagt ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc
    10921 aatgaacgta ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca
    10981 gctttatgaa cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt
    11041 gaatattcaa taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat
    11101 ggttttttca ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat
    11161 gtcaaaaagt tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca
    11221 tttggggata cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag
    11281 actggtacat caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt
    11341 ggtcaattag acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata
    11401 aatgtgacca tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa
    11461 ggggatcgga cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat
    11521 aaagaagcgt tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa
    11581 gtaattttct tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa
    11641 tagaaaaggg ttacattttt atatgaagct gtatttttat ataacattac tggaggagat
    11701 attgtggata cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa
    11761 aacgagccgc tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc
    11821 ggagcagttg gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt
    11881 gatttaccgg taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact
    11941 gcgacagaaa aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat
    12001 gcaacttctc gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac
    12061 gaacacggtc ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa
    12121 gaagatggtt ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag
    12181 acgcagacac ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta
    12241 attatggaag gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct
    12301 ttttcagtcg tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc
    12361 gaagccgcaa gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa
    12421 gaaaaattga tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca
    12481 ttttgtcttc aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg
    12541 aacggagtcc ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca
    12601 tccctagacc agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac
    12661 ccttcagtta cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga
    12721 gaaaacaaga tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta
    12781 tccatatcct tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta
    12841 atgaggaaag cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag
    12901 taccagcatt gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga
    12961 tacggaataa aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt
    13021 taaatgaaac agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg
    13081 taagccgttt ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc
    13141 ccgaataaaa aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa
    13201 aagaaatgaa aaaagaatat gaaacagtga atgattttct gcaatggcag tacttagaag
    13261 ttccgccatt gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag
    13321 aaatgccaga tcaaacgatt agcggattgt ttaattactt taacaaataa atatttattc
    13381 cgaaaagtga aaaaacatat aaaaaaacac ataaaactaa aaaattatca aagaatcctg
    13441 gtgcaactga aagaatttgc aacgggattc tttcaactta tttgttattt ttttctaaaa
    13501 aatattaaag attgttaaat ttcactattt catttcaaat tccattgcct aatcattagg
    13561 aaaagtctat catgaactcg taaatagaat atagtaataa ccttgttagg tgaggctcct
    13621 atacaaacat aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact
    13681 gttggattaa ggctttttcc aatagctaag aatgttttct ttacgttgta tagtgctaaa
    13741 actcggacga tgagatgatg ctaggtcaga attcttgttc gaaaaggttt ctgacctttt
    13801 ctttatattt ttgtgactga cttatttgat gagacaaact cgctcctcac ctgacaagtg
    13861 gattgctgtg tgaggaggag atgcggtgaa aaaagatcga ttggtggatt ttgggtcctt
    13921 atttgacttt gtccatgatc ggtcttttag aagtatacag tgccagttct tatcgtttat
    13981 tgcaagccga tgaaaataca aaaagtctat tattgcgtca actaattttc atatttttga
    14041 gttggagcgt gatcttctta gctcgttcag tcaaactaca ctatttactt caccctaaaa
    14101 tagcaggata cggtttagcc ttatcgattt tctttttagt attagtaaga atagggatat
    14161 tcggtgtcac tgtcaacggc gcacaacgtt ggatctctct gtttggcatt caattccagc
    14221 cttctgaact ggcaaatctt tttttgattt tttatttaag ctggtttttt cgtgacggaa
    14281 atagtagccc aaaagatcta aaaaaaccat tcctgattac agtaggtata acttttctga
    14341 ttttatttca gccaaagatt gctggagcat tgatgatcct ttcgattgcg tgggtcatat
    14401 tttgggcagc ggcggttcca tttaaaaaag ggatctatct aatcgttact ttttctgcat
    14461 tgctgattgg agcagcaggc ggggtattat atttaggaaa taaaggttgg cttccacaga
    14521 tgtttaatca tgcttatgaa aggatcgcga ctttaagaga ttcttttatc gatagtcatg
    14581 gtgcgggcta tcaaatgacc cattcgtttt atgcactata taatggtggc atatttgggc
    14641 ggggattagg aaatagtatc acaaaaaaag ggtatcttcc agagacagaa acagatttta
    14701 ttttttcgat tattacagaa gagcttgggc taatcggtgc attgtgtgtg cttttcttat
    14761 tgttctcatt atgcatgaga atcttttgtt taagcagtcg ctgtaaaaat cagcaagctg
    14821 gattgtttct tttaggattc ggaactttgc tattcgttca gacgataatg aacgtgggaa
    14881 gtatcgcggg gctgatgcca atgactggtg tgccattgcc atttgttagc tacggaggaa
    14941 caagttatct gatcctttct ttaggaatcg gaatcacgtt gaatatttct tcaaagatac
    15001 aggccgaaga gttggcgctc tatcgacctg aaaaacaata aagagaaact gagaaactct
    15061 cagtttgaat tatcataaag tacaatcttt tatttttcaa gcgagaggtt cttttagaaa
    15121 gaggaattcg caatgaaagt atctcaaaaa atcatattat ctattttgtt actgcttaca
    15181 ttagtagttg gctatttttc gattgagttt atccatggat tttcgtctgc aaaacagagt
    15241 tcaactgtga aaaaggtaga gccgaaaagt atccctacca cactaaatgt ggctttgatt
    15301 ggttcagatg ctcgttcgaa agacgaaaac ggtcgctcgg attcacttct ggttgcacaa
    15361 tacaaccaga aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat
    15421 attccaggtt acggaatgaa taagataaat gcagcttact cttatggggg agttgattta
    15481 ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca
    15541 tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca
    15601 gaaaaatctc tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt
    15661 aatactttac tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc
    15721 agaaggcagc agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata
    15781 ttcaagctac ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa
    15841 agtgtgcttc tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta
    15901 taaatagtga aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac
    15961 aagcacggca aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct
    16021 gcgggtggcg gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga
    16081 gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca
    16141 gaaatgacat caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat
    16201 aaataccaga atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa
    16261 gtagataaaa aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca
    16321 ttaggtgaat tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc
    16381 acgatcaact ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc
    16441 ctgaccacac aagaagcagc aagagggaat attatagatc gaaatggtga accattagca
    16501 acaacaggca aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa
    16561 aaaacagcca atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat
    16621 caggcgattt cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat
    16681 ggagcaacgc cagaacttcc agctggagct accattcaag aagtagacgg cagatattat
    16741 cctttgggtg aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat
    16801 attgataaga atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg
    16861 gcttttgata aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac
    16921 ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg
    16981 acaatcgatg cgaaggcaca aaaaacagct tttgacagtc taggaggaaa agctggatca
    17041 actgttgcga caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat
    17101 gatccaaaca aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat
    17161 cctgaacatc cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa
    17221 atgattacag cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg
    17281 atcaacgggc ttaaatggca aaaagacagt tcttggggat cttatcaagc aacgcgtgtc
    17341 agtagtgatg tatcacaagt agacttaaaa actgctttga tctattccga taatatatat
    17401 gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc
    17461 atttttggtg aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa
    17521 gatagcttta actcagatat cttgctagct gatactggat atggacaggg cgaacttcta
    17581 attactccta tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc
    17641 tatcctaaat tgattgcaga taaagagaca aaagataaga agaatgtcat cagcgaaaca
    17701 gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca
    17761 cattctcttt ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa
    17821 gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa tcttgataac
    17881 caaggttata tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa
    17941 cgagcatcgg aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa
    18001 gtacagtaaa aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca
    18061 cgatgtctct ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa
    18121 atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga
    18181 atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat
    18241 ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc
    18301 aatctgagca ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat
    18361 agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac
    18421 aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc
    18481 tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag
    18541 cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat
    18601 agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac
    18661 taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc
    18721 attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa
    18781 aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt
    18841 tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa
    18901 gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac
    18961 cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag
    19021 agataaatca ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa
    19081 gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc
    19141 aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat
    19201 cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca tcattccttt
    19261 gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac
    19321 tcacaaaaaa accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat
    19381 tttaataact aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat
    19441 ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact
    19501 tttgaatgga ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac
    19561 actccaactt ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat
    19621 aacagctaat ccaagaagaa tcggcatgat agaaacgatt gcaggtgcaa aagcatagac
    19681 gatcagagct aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt
    19741 tttcctttta aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc
    19801 ggtgatcaga taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc
    19861 agaaataata taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt
    19921 ctcaaacata taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa
    19981 attaaatgtt acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg
    20041 atcttttcca tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt
    20101 tctttcatca agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc
    20161 aaggaacgtg aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt
    20221 ttttgtagtt gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca
    20281 tctacttcct cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg
    20341 cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt
    20401 caaattattt actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa
    20461 aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt
    20521 acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct
    20581 cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg
    20641 aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc
    20701 atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag aatgaatact
    20761 cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct
    20821 acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca
    20881 agagagacta gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga
    20941 acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca
    21001 ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa
    21061 gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca
    21121 cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta
    21181 ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg
    21241 taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta
    21301 ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca
    21361 agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca
    21421 ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg
    21481 ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca
    21541 tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc
    21601 ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc
    21661 tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt
    21721 tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta
    21781 atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa
    21841 acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg catcgaatct
    21901 cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt
    21961 ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt
    22021 gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc
    22081 tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga
    22141 caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca
    22201 ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga
    22261 tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata
    22321 cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct
    22381 gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca
    22441 acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa
    22501 acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt
    22561 tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt
    22621 ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata attattgtaa
    22681 tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac
    22741 gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg
    22801 gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt
    22861 gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt
    22921 ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta
    22981 aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac
    23041 aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg
    23101 ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg
    23161 aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg
    23221 cagaaacaag attaattttg gaaacatacg taccactttg aggtatcgta tacacgagtt
    23281 cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt
    23341 cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt
    23401 tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat
    23461 catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta
    23521 actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa
    23581 attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa
    23641 caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga
    23701 ccgtgcatca ttaagagaaa atggacaaaa aatcctgtct ggatccattt cggtggcgga
    23761 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    23821 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    23881 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    23941 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    24001 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    24061 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    24121 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    24181 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttaacaaaac
    24241 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    24301 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    24361 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    24421 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    24481 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    24541 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    24601 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    24661 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    24721 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    24781 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    24841 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    24901 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    24961 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    25021 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    25081 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    25141 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    25201 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    25261 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    25321 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    25381 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    25441 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    25501 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    25561 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    25621 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    25681 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    25741 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    25801 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    25861 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    25921 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    25981 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    26041 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    26101 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    26161 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    26221 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    26281 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    26341 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    26401 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    26461 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    26521 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    26581 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    26641 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    26701 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    26761 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    26821 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    26881 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    26941 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    27001 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    27061 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    27121 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    27181 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    27241 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    27301 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    27361 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    27421 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    27481 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    27541 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    27601 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    27661 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    27721 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    27781 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    27841 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    27901 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    27961 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    28021 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    28081 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    28141 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    28201 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    28261 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    28321 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    28381 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    28441 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    28501 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    28561 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    28621 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    28681 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    28741 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    28801 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    28861 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    28921 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    28981 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    29041 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    29101 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    29161 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    29221 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    29281 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    29341 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    29401 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    29461 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    29521 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    29581 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    29641 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    29701 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    29761 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    29821 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    29881 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    29941 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    30001 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    30061 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    30121 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    30181 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    30241 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    30301 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    30361 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    30421 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    30481 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    30541 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    30601 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    30661 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    30721 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    30781 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    30841 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    30901 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    30961 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    31021 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    31081 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    31141 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    31201 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    31261 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    31321 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    31381 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    31441 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    31501 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    31561 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    31621 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    31681 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    31741 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    31801 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    31861 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    31921 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    31981 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    32041 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    32101 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    32161 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    32221 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    32281 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    32341 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    32401 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    32461 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    32521 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    32581 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    32641 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    32701 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    32761 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    32821 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    32881 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    32941 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    33001 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    33061 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    33121 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    33181 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    33241 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    33301 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    33361 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    33421 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    33481 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    33541 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    33601 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    33661 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    33721 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    33781 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    33841 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    33901 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    33961 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    34021 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    34081 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    34141 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    34201 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    34261 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    34321 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    34381 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    34441 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    34501 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    34561 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    34621 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    34681 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    34741 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    34801 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    34861 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    34921 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    34981 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    35041 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    35101 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    35161 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    35221 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    35281 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    35341 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    35401 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    35461 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    35521 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    35581 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    35641 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    35701 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    35761 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    35821 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    35881 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    35941 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    36001 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    36061 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    36121 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    36181 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    36241 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    36301 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    36361 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    36421 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    36481 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    36541 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    36601 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    36661 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    36721 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    36781 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    36841 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    36901 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    36961 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    37021 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    37081 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    37141 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    37201 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    37261 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    37321 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    37381 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    37441 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    37501 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    37561 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    37621 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    37681 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    37741 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    37801 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    37861 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    37921 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    37981 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    38041 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    38101 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    38161 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    38221 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    38281 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    38341 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    38401 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    38461 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    38521 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    38581 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    38641 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    38701 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    38761 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    38821 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    38881 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    38941 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    39001 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    39061 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    39121 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
    39181 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    39241 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    39301 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    39361 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    39421 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    39481 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    39541 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    39601 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    39661 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    39721 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    39781 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    39841 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    39901 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    39961 cggagatacg gacactccta cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    40021 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    40081 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    40141 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    40201 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    40261 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    40321 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    40381 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    40441 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    40501 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    40561 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    40621 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    40681 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    40741 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    40801 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    40861 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    40921 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    40981 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    41041 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    41101 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    41161 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    41221 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    41281 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    41341 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    41401 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    41461 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    41521 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    41581 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    41641 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    41701 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    41761 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    41821 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    41881 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    41941 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    42001 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    42061 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    42121 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    42181 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    42241 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    42301 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    42361 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    42421 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    42481 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    42541 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    42601 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    42661 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    42721 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    42781 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    42841 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    42901 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    42961 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    43021 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    43081 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    43141 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    43201 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    43261 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaaagggat ggctagctac
    43321 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    43381 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    43441 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    43501 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    43561 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    43621 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    43681 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    43741 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    43801 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    43861 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    43921 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    43981 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    44041 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    44101 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    44161 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    44221 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    44281 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    44341 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    44401 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    44461 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    44521 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    44581 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    44641 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    44701 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    44761 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    44821 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    44881 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    44941 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    45001 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    45061 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    45121 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    45181 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    45241 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    45301 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    45361 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    45421 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    45481 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    45541 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    45601 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    45661 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    45721 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    45781 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    45841 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    45901 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    45961 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    46021 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    46081 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    46141 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    46201 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    46261 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    46321 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    46381 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    46441 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    46501 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    46561 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    46621 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    46681 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    46741 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    46801 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    46861 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    46921 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    46981 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc
    47041 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    47101 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    47161 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    47221 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    47281 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    47341 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    47401 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    47461 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    47521 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    47581 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    47641 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    47701 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    47761 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    47821 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    47881 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    47941 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    48001 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    48061 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    48121 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    48181 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    48241 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    48301 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    48361 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    48421 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    48481 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    48541 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    48601 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    48661 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    48721 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    48781 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    48841 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    48901 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    48961 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    49021 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    49081 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    49141 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    49201 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    49261 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    49321 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    49381 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    49441 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    49501 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    49561 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    49621 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    49681 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    49741 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    49801 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    49861 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    49921 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    49981 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    50041 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    50101 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    50161 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    50221 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    50281 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    50341 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    50401 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    50461 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    50521 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    50581 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    50641 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    50701 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    50761 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    50821 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    50881 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    50941 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    51001 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    51061 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    51121 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    51181 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    51241 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    51301 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    51361 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    51421 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    51481 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    51541 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    51601 atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc
    51661 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    51721 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
    51781 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    51841 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    51901 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    51961 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    52021 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    52081 aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    52141 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
    52201 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    52261 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    52321 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    52381 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    52441 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    52501 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    52561 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    52621 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    52681 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    52741 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    52801 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    52861 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    52921 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    52981 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    53041 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    53101 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    53161 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    53221 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    53281 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    53341 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    53401 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    53461 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    53521 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    53581 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    53641 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    53701 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    53761 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    53821 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    53881 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    53941 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    54001 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    54061 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    54121 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    54181 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    54241 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    54301 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    54361 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    54421 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    54481 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    54541 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    54601 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    54661 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    54721 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    54781 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    54841 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    54901 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    54961 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    55021 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    55081 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    55141 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    55201 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    55261 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    55321 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    55381 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    55441 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    55501 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    55561 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    55621 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    55681 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    55741 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    55801 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    55861 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    55921 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    55981 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    56041 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    56101 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    56161 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    56221 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    56281 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    56341 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    56401 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    56461 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    56521 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    56581 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    56641 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    56701 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    56761 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    56821 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    56881 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    56941 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    57001 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    57061 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    57121 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    57181 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    57241 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    57301 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    57361 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    57421 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    57481 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    57541 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    57601 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    57661 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    57721 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    57781 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    57841 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    57901 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    57961 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    58021 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    58081 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    58141 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    58201 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    58261 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    58321 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    58381 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    58441 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    58501 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    58561 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    58621 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    58681 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    58741 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    58801 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    58861 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    58921 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    58981 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    59041 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    59101 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    59161 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    59221 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    59281 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    59341 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    59401 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    59461 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    59521 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    59581 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    59641 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    59701 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    59761 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    59821 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    59881 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    59941 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    60001 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    60061 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    60121 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    60181 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    60241 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    60301 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    60361 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    60421 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac
    60481 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    60541 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    60601 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    60661 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    60721 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    60781 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    60841 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca
    60901 tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta
    60961 tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca
    61021 tatagtttag taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag
    61081 gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag
    61141 actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg
    61201 gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata
    61261 ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag
    61321 cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt
    61381 atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag
    61441 aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac
    61501 aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc
    61561 taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact
    61621 aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag
    61681 ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga
    61741 tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca
    61801 attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc
    61861 agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga
    61921 acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac
    61981 actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct
    62041 agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga
    62101 aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg
    62161 agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt
    62221 accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa
    62281 ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa
    62341 aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca
    62401 aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa
    62461 agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta
    62521 taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac
    62581 tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga
    62641 acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg
    62701 aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat
    62761 tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa
    62821 acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag
    62881 cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc
    62941 tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg
    63001 acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt
    63061 ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc
    63121 acttagccgg caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg
    63181 aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt
    63241 tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga
    63301 atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat
    63361 tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg
    63421 ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg
    63481 gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact
    63541 tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat
    63601 tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat
    63661 ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg
    63721 ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga
    63781 cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
//
LOCUS       DAFFQY010000003        60122 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFFQY010000003 DAFFQY010000000
VERSION     DAFFQY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.003"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(105..1124)
                     /locus_tag="LRB02_000147"
     CDS             complement(105..1124)
                     /locus_tag="LRB02_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4658391.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="LRB02_000148"
     CDS             complement(1146..2153)
                     /locus_tag="LRB02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL4658392.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="LRB02_000149"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="LRB02_000149"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL4658393.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="LRB02_000150"
     CDS             complement(2804..5098)
                     /locus_tag="LRB02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL4658394.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="LRB02_000151"
     CDS             5358..7523
                     /locus_tag="LRB02_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4658395.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="LRB02_000152"
     CDS             7827..8660
                     /locus_tag="LRB02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL4658396.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="LRB02_000153"
     CDS             complement(8762..10462)
                     /locus_tag="LRB02_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658397.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="LRB02_000154"
     CDS             10969..12129
                     /locus_tag="LRB02_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4658398.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="LRB02_000155"
     CDS             12145..12588
                     /locus_tag="LRB02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4658399.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="LRB02_000156"
     CDS             complement(13071..13601)
                     /locus_tag="LRB02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL4658400.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="LRB02_000157"
     CDS             13706..14257
                     /locus_tag="LRB02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4658401.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="LRB02_000158"
     CDS             complement(14410..14883)
                     /locus_tag="LRB02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4658402.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="LRB02_000159"
     CDS             15021..16493
                     /locus_tag="LRB02_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4658403.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="LRB02_000160"
     CDS             16490..17200
                     /locus_tag="LRB02_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL4658404.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17432..17716
                     /locus_tag="LRB02_000161"
     CDS             17432..17716
                     /locus_tag="LRB02_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL4658405.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(17997..18416)
                     /locus_tag="LRB02_000162"
     CDS             complement(17997..18416)
                     /locus_tag="LRB02_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL4658406.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18418..19002)
                     /locus_tag="LRB02_000163"
     CDS             complement(18418..19002)
                     /locus_tag="LRB02_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL4658407.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19049..20524)
                     /locus_tag="LRB02_000164"
     CDS             complement(19049..20524)
                     /locus_tag="LRB02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL4658408.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20705..21016
                     /locus_tag="LRB02_000165"
     CDS             20705..21016
                     /locus_tag="LRB02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658409.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21131..23467)
                     /locus_tag="LRB02_000166"
     CDS             complement(21131..23467)
                     /locus_tag="LRB02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4658410.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23609..24277
                     /locus_tag="LRB02_000167"
     CDS             23609..24277
                     /locus_tag="LRB02_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4658411.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24280..25323
                     /locus_tag="LRB02_000168"
     CDS             24280..25323
                     /locus_tag="LRB02_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4658412.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25560..26441)
                     /locus_tag="LRB02_000169"
     CDS             complement(25560..26441)
                     /locus_tag="LRB02_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4658413.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26600..27328
                     /locus_tag="LRB02_000170"
     CDS             26600..27328
                     /locus_tag="LRB02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL4658414.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27484..27801
                     /locus_tag="LRB02_000171"
     CDS             27484..27801
                     /locus_tag="LRB02_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4658415.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27794..28402
                     /locus_tag="LRB02_000172"
     CDS             27794..28402
                     /locus_tag="LRB02_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL4658416.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28457..29248
                     /locus_tag="LRB02_000173"
     CDS             28457..29248
                     /locus_tag="LRB02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL4658417.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29386..29874
                     /locus_tag="LRB02_000174"
     CDS             29386..29874
                     /locus_tag="LRB02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL4658418.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29925..30797
                     /locus_tag="LRB02_000175"
     CDS             29925..30797
                     /locus_tag="LRB02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL4658419.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            30973..31335
                     /locus_tag="LRB02_000176"
     CDS             30973..31335
                     /locus_tag="LRB02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4658420.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31344..32096
                     /locus_tag="LRB02_000177"
     CDS             31344..32096
                     /locus_tag="LRB02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658421.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISRILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32110..33912
                     /locus_tag="LRB02_000178"
     CDS             32110..33912
                     /locus_tag="LRB02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658422.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34189..35230
                     /locus_tag="LRB02_000179"
                     /pseudo
     CDS             34189..35230
                     /locus_tag="LRB02_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(35282..37426)
                     /locus_tag="LRB02_000180"
     CDS             complement(35282..37426)
                     /locus_tag="LRB02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL4658423.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37783..39156)
                     /locus_tag="LRB02_000181"
     CDS             complement(37783..39156)
                     /locus_tag="LRB02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4658424.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39156..39551)
                     /gene="rbsD"
                     /locus_tag="LRB02_000182"
     CDS             complement(39156..39551)
                     /gene="rbsD"
                     /locus_tag="LRB02_000182"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL4658425.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39579..40496)
                     /gene="rbsK"
                     /locus_tag="LRB02_000183"
     CDS             complement(39579..40496)
                     /gene="rbsK"
                     /locus_tag="LRB02_000183"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL4658426.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40604..41617)
                     /locus_tag="LRB02_000184"
     CDS             complement(40604..41617)
                     /locus_tag="LRB02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL4658427.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41698..42297)
                     /locus_tag="LRB02_000185"
     CDS             complement(41698..42297)
                     /locus_tag="LRB02_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4658428.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42483..43184
                     /locus_tag="LRB02_000186"
     CDS             42483..43184
                     /locus_tag="LRB02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658429.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43194..45905
                     /locus_tag="LRB02_000187"
     CDS             43194..45905
                     /locus_tag="LRB02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658430.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46356..47984
                     /gene="treC"
                     /locus_tag="LRB02_000188"
     CDS             46356..47984
                     /gene="treC"
                     /locus_tag="LRB02_000188"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL4658431.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48044..49225)
                     /locus_tag="LRB02_000189"
     CDS             complement(48044..49225)
                     /locus_tag="LRB02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4658432.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49477..51000
                     /locus_tag="LRB02_000190"
     CDS             49477..51000
                     /locus_tag="LRB02_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL4658433.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51069..51797)
                     /locus_tag="LRB02_000191"
     CDS             complement(51069..51797)
                     /locus_tag="LRB02_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4658434.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51810..53091)
                     /locus_tag="LRB02_000192"
                     /pseudo
     CDS             complement(51810..53091)
                     /locus_tag="LRB02_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(53232..53834)
                     /locus_tag="LRB02_000193"
     CDS             complement(53232..53834)
                     /locus_tag="LRB02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL4658435.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54079..54285
                     /locus_tag="LRB02_000194"
     CDS             54079..54285
                     /locus_tag="LRB02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658436.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54597..55316
                     /locus_tag="LRB02_000195"
     CDS             54597..55316
                     /locus_tag="LRB02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4658437.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55397..56080)
                     /locus_tag="LRB02_000196"
     CDS             complement(55397..56080)
                     /locus_tag="LRB02_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL4658438.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56298..58484
                     /locus_tag="LRB02_000197"
     CDS             56298..58484
                     /locus_tag="LRB02_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL4658439.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            58830..59240
                     /gene="spx"
                     /locus_tag="LRB02_000198"
     CDS             58830..59240
                     /gene="spx"
                     /locus_tag="LRB02_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL4658440.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            59254..59610
                     /locus_tag="LRB02_000199"
     CDS             59254..59610
                     /locus_tag="LRB02_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL4658441.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        19337 a        10643 c        12110 g        18032 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccat acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagta
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 ccacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg agaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt ccggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ttcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tcctttttgc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctattttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaccaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata cacccaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgtt
    15361 tgatccaagc ttgcggtgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccacc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accgtttaag
    17221 gaagaggttg tgagcctgcc gtttaactct gagtcacaag gagcaatttc aaaaaacagc
    17281 ttctcatgtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    17341 attcgaaaaa aggaactgcg acaagacttt tgtcacagtt ccttcttttt tttgctatgc
    17401 tatactagat gtaaaaaata ggagaaataa aatgagggaa atttattctt ttgaatcaaa
    17461 aatctatgct tccgaagtcg gcaagggcgg ggcatatatt atttttccgt atgatataag
    17521 aaaagaattc ggtgctggaa gagttaaagt ccaagcaact tttgacaatg tgccatatga
    17581 aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt tcagtctgct acatccttgg
    17641 actacgaaaa gatattcgta aaaacatcgg aaaagaaatc ggtgatattg tagcagttac
    17701 agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt cacaacctct tgataaacgg
    17761 tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaacccatg
    17821 tttacaaaat ttttcttact actcgaaacg aagcggcttt atcacaacct cttgataaac
    17881 ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg gcaaaatatg agaagctatt
    17941 tttccatttt tttctctcat actcaggtca taacgctgat ttcacaacct cttctttcat
    18001 aatggttttt caccatcttt ataataaact ggttctttga tatggaaatc tattttttcc
    18061 aaccaatcta aaatcttgta acctaactct tctgacatcg gaaaagaggc gttgatttgt
    18121 gcattggaga tatggtcaac atggataccg gaagtgatcg tacaacttcc cggaacggca
    18181 gttttgatga tcacagctat tttttcggct aagacatcgt ctttatggaa ccgaccatcg
    18241 tggctaggga aacgtacagt ttgcatctct gtatcacgag aaaatgtagt gactgttccg
    18301 atatgtggtg tatctccgcc tgttaaagtg atcaataagt ctttaccgat tactttaaca
    18361 gcagcttgga tcgtgaaacc tagtttggtt acttcaacta tcgtttcttt ttccatatta
    18421 atcaccctgc caacgttttg aatgctcatt tacaatttca aattgatcca aaagtttcat
    18481 tgtatggtga ttaacaagat cttggatcgt ttgaggctga ttataaaaag caggaattgg
    18541 tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag ttttccaagt gaatggcatt
    18601 taatggcgtt tcacgaggaa caatgactaa ctttcgttgc tccttcattg ttacatcagc
    18661 agcacgtgca atcaggttgt cgcctaaacc aacagagata cctgcgactg ttttcatact
    18721 tgccggtacg atgatcattc catcggtcaa aaatgatccg ctggcgacag cagcacctaa
    18781 atctttgttt gaataatggt aatcaaacag gctttcgaat tcagttagtg ataagtcgga
    18841 ttccagcttt aagttctgtt ttgcccatgg actgatgatt ccgtgtgttt ctatcatcgg
    18901 atattctttt agtttcttca ctaggttgat ggcataaatg gtgcctgaag cgccacttac
    18961 accaatcacg atttttttct tccgttgtgc caattgttcc attttccgca ctccttttta
    19021 tgatgtcaaa acagttgcag atcattcatt atttcaaata tttttcccaa tcttctactt
    19081 cttggaattt tgcacgtaca aaatgttctt tcatatcgaa aggaactgta ccgtcaaaga
    19141 tagtttttgc acttatccca cggaaacgaa tagagccagg attatattct ggtaattctg
    19201 atgggtccaa tggatggttc ctcattcctg ggatcacgat gatatcttta tctccttgga
    19261 aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa aatatcaaca tcttcatcga
    19321 ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc aagaatagct gcttgacgct
    19381 gaataccttc atccgcttcg ttttctttac ggatttgcat gatggtcatc aatttaccac
    19441 cacctgcagg aggattgtag acatttacta cttttcctgg aatagcttta tcgaccaagg
    19501 caagaatact tgcttctgta ggaataccgg ccatagatac gtgttcttca ctgggaccaa
    19561 tcgttgtttg catgattgga ttgtcttttc ggtgagtaac tgctttgact ttcactactt
    19621 gtacagctgg attagcatcc ccagtatagc caggaaattc tggcattgct tttcctgagt
    19681 tcgtgttgat atcttcttgc attgtttcat ttggcatgat ttctgcttca atgatgaatt
    19741 cagcacgagc aatacccact tcatcgactg ctataccatc aacgatttga ataggttcgt
    19801 tacgcaaagc tcctgcacac catagttcat tgtagccaag tggagtggta ggtggttcga
    19861 aagtcgtgcc aatggcaatt gctggatcta aaccaatatt gatagtgata ggcattggtt
    19921 tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg gcgtccgcca ggcatgatgt
    19981 acataccgat cgtatcttta tcttctagaa ccatacgatg gatcgttaca tcgctcattg
    20041 ttttttctgg atctgatcct aagactacac ccattgtgat gtatggacca gcatcttcag
    20101 gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa gtctgcatcg gtagctagat
    20161 gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt cacaggtttg attggatttt
    20221 ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc atggtgcaaa atttcaccta
    20281 cacgtttacg actagccatt gttccgatgt tcacacgtgt gccggggaaa cctttgatat
    20341 tgttgaaagt catagttggt ccttcttgag tcggacgttt tactgtaccg ccagcaccga
    20401 tataacggta aacaccagat aattctgctt caggatcgat ttcaatatct gtttcgtgat
    20461 attgacctgg aagtgttttt agttcttcga tgacttttct taaatcatac ggttgatctg
    20521 tcatgagaga ttcctcctta gttgtcttta cctatctata gtaaaaggtg aaaaagagga
    20581 aaacaattca acttgttcgg taaagtattc tacaatggaa tggatgcgtt tttttgataa
    20641 tagaataaac tatgatatac ttatcactaa aagaaagcgc atacttagtg ttggaggaag
    20701 gaaagtgaaa aaaatggagg agtcatttcc taaagcagta aaagtggaaa atatagcgaa
    20761 tattttgaaa gttacatttg aaaacggaga agtaaaatac gtgaaatctc attggacaga
    20821 agagataacc gatgccttgc aatttggaaa aaaaggaaga ggaaaaagaa aaaatcttct
    20881 cgcactttct cgtaatatgt ggattgggac tgaagtaacg attgaagcag atggaacagt
    20941 attcataaat ggaaaagaca ggtatacgcc agaagagttg tggtacaaag gaaagaaaag
    21001 tataccagaa ttataaaaaa taattaaaaa aaacagcgcg aaacaaggaa catattactt
    21061 cctcgtttct tgctgttttc tactatttca gcgctcttat gactcaaatc taaatgtggt
    21121 tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc ttttttcgct gccattcgtg
    21181 caggaagatg accgccaagc atcgtcaata tagtagagac gatcactaga ataatcgcat
    21241 ggacaggatt tagttgagca acatttttta gatccgtcat gttgtataag atgctattga
    21301 tagggaacgt agctagccat gcaataaaca cacctaatat cccagaagaa atacctaaaa
    21361 tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct aaagctttta
    21421 gtacaccgat ttcttttgtc cgttcaatca cagatgtata agtaatgata ctgatcatga
    21481 tcatacttgt tactaaagag atccctgcaa aagcaataag gacataagtg atagcatcca
    21541 tcaatccgcc agtcagttca gtcatcgttc cagctaaatc agtataaatg attttatctt
    21601 catctgattt gcctttattg taatcatcta gataatccaa gatcttttct ttgtcttcaa
    21661 aattgttcgg ataaatcatg atacttgaag gcaagctgtc tccacctaaa taactaagca
    21721 atgtttgttt ggcattttcg tcaacttttt cagtagttaa gacatttaca ccactgtctc
    21781 tttgagcttt gacgatttct gattctttgt tttcgttgac gatttgagtc gttaattgat
    21841 cgctataagc gattcctgga gataagagat tcattgtaga agaagatttc acgcgtagga
    21901 tccccgagat cttcaattca tcagaagcat tctgatacat cgcatcataa tctgtattag
    21961 ggataaaatt cccagttgga agttttgtat agtaagtatt gttgtttact aatttgaatg
    22021 ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc tttcacatca aatccaagat
    22081 tcttcaatgc gttgatatta gtattattat tgccgtctac gatcagcacg acatctgttg
    22141 ctgaggctgg ataacttccg gcaagtaaag aataattgtc ttttaaaaag ttttctttgc
    22201 tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct gacatcgtac
    22261 tagataaaga aagagattcc gtatcaggat tttgattcga gaagctgaca ggctgtactt
    22321 tgccatttac atcacgtaaa agattgattc ctgtagtacg ggtaaatccg atattattac
    22381 ttaagttagg atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc ttattcgtgt
    22441 gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt tttgctgtct ggataatccg
    22501 ctttgcttgc acccaatccg gcatcgtcac gagtctggtt agtcgttacc ttagaaatag
    22561 taatcggaaa tttcgccatc gtttcagctt gcgtgttatc gatttgtttt tggaatccag
    22621 aagaaaggga cagaacgatc gcgattccga tgatcccaat actggaagca aaggaggtca
    22681 agaaagtacg tccttttttt gttcggatat tgttgaagga aagctttaac gctgtccaaa
    22741 aactcatctt tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg atgtgaggat
    22801 gggaatctgt taagatctta ccatcagaaa attcaatgat tcgatctgcg tattgatggg
    22861 ccagttcagg attatgtgtg accatgatca ctaatttttc tttcgataac tcttggatca
    22921 attccatgat ctggatactt gtttctgtat ctaacgctcc ggttggttca tcgcacaaaa
    22981 ggatatctgg gtcattggct agtgctcgag cgatggccac tcgctgcatt tgtccaccag
    23041 ataactgatt tggcttttta tgcatatgat ttgtcaagcc gactcggtgc aatgcatctt
    23101 ctgcccgttt tcgtttttcg tctttagaaa caccacttaa tgtcatccca agttcaacgt
    23161 tttcaataat gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa ccgatagagt
    23221 tgtttcggta ggcatcccag tcactgtcct taaagtcttt agtagacttt ccgttgatta
    23281 ccatatcacc ggaatcataa ttatctagtc caccaatcac attcagcata gtcgttttac
    23341 cagaaccact aggacctaaa atcgctacga attctttttg tctgaaagcg acagacacac
    23401 catccaatgc tttggtggtg gtttcgccga ctttataata tttttttata tcttttaatt
    23461 gaagcataat cacccttctc ccgtattgaa atattcggta tactaataag tatatcagaa
    23521 caatgatcgt agtatacgaa ataaatgtta actgaagatg aactacaaga aaaaatctag
    23581 aaatttttca aaaaacagag ggagatcaat gaatcgtatt ttagtagtag aagatgatcc
    23641 gcaattacag ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag tcatcaccgc
    23701 attgaatgga gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat taatcattac
    23761 agatatcatg atgccgaaaa tggatggcta tgacttgcta gactcattac gtagcaccag
    23821 atttgacaca cctgttctaa tgattacggc aaaggccgat tttgaggata agaaacgcgg
    23881 gttcaaatta ggagcagatg attatatgac aaaacccatc gatgtcaatg agatgatcct
    23941 acgtgtggaa gcattgcttc gacgttcaaa gatcaattac agcagtagct tgataatcgg
    24001 acaaacaaaa ttagagctag atgcttatac agtgttacaa gatcagcatt cgttgattct
    24061 gcctcaaaaa gaatttctgc ttctgtataa attactgtct tatcccaaca agatcttcac
    24121 tcgccaacag ttgatggatg atatttgggg attagatacg aatacagaag aacgaacgat
    24181 cgatgtacat atcaaacgtt taaggacccg ttttgaaaag acggaagatt ttcaaatcat
    24241 cacagtacgg ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa gctattttca
    24301 caattatgga tctattttgc tagtattgtc ttcatttcga tcttagtgac actgctgtgt
    24361 tttctcggtt ttattttctt attgtctcga cattctatct cgcctgctga atcggctgga
    24421 aaaccaacgt tatacccgtt attcgtcttt gcggggttca gtatggtcgt gggtacagga
    24481 atatcgattt ttgttgggcg gcgtatcctg catccaatca gtgcgctagg gacaaatatg
    24541 agtctggtag caacgggaga tttttcgatt cgaatggatg aacagcagaa agtagcagaa
    24601 gtacagcaac tgtataaaga tttcaacgtt atggtccaag aactcaatag tatcgagaca
    24661 ctgaggaatg actttgtgtc cagtgtatcc catgaattca aaacaccact agcaacaatc
    24721 caaggatatg ttcaattgtt acaagcccca aatctctctg acgaagaacg ccaaatattt
    24781 ttatatcgga ttatcgaaag tatcacgcag ctttcacaat taacagagaa catcttaaaa
    24841 ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg attagatgaa
    24901 caaatcagag aagtaatcgt tttttttcag cctaaatggg agaaagaaca gctagaattg
    24961 gatatcgaac ttgctgcagt aaattacaca ggcaatgaag agtttttgta ccaagtgtgg
    25021 ctgaatatca tggataacgc cataaagtac aatcaaatca atggacagat tcatatcaaa
    25081 ctatttgaga cagccacaga gattgtactg gaagtgaccg attcaggtgt aggaatgaat
    25141 gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt
    25201 tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt
    25261 attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa
    25321 taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt
    25381 ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt
    25441 attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga
    25501 gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct
    25561 tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg
    25621 actttacgag taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta
    25681 ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt
    25741 gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat
    25801 ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc
    25861 aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga
    25921 tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt
    25981 ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg
    26041 taagttaatt cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc
    26101 aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata
    26161 tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat
    26221 tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt
    26281 tttctattgg cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt
    26341 ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt
    26401 tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt
    26461 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
    26521 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
    26581 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgtt
    26641 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
    26701 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
    26761 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtt tgattgttca
    26821 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
    26881 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
    26941 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
    27001 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
    27061 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
    27121 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
    27181 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
    27241 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
    27301 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
    27361 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
    27421 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
    27481 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
    27541 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
    27601 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
    27661 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
    27721 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
    27781 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
    27841 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
    27901 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
    27961 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
    28021 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
    28081 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
    28141 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
    28201 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
    28261 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
    28321 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
    28381 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
    28441 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
    28501 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
    28561 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
    28621 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
    28681 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
    28741 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
    28801 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
    28861 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
    28921 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
    28981 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
    29041 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
    29101 ccaaacgtct ggtaaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
    29161 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
    29221 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
    29281 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
    29341 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
    29401 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
    29461 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
    29521 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
    29581 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
    29641 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
    29701 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
    29761 ttggattatt taccgcaggc agccggaggg acacaggcaa gcggttggta catcactgaa
    29821 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt
    29881 aacattagat atttattgaa ggataaagga gcgcaggaag tagcatgaga gaaaaaagag
    29941 cattttatat caatggttat gcagggattg tcggattggt cgtattagct ttagttggat
    30001 tgtttttctt ctatttaggt atgtggcaag cgaaagtttg ggcacttttt ttaagtattt
    30061 ttctttggtt gatcacgtta ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag
    30121 caaaagtaat tttgtttttt ggacaatatt taggtacgat tcgagaaaat ggctttttcc
    30181 ttacgatacc tttggcacaa aaaatgactg tttcgttgaa agttcggaat ttcaatagtt
    30241 ctgttttaaa agtcaatgat ttggatggaa accccatcga aatctcagca gtagtagtct
    30301 ttaaagtcgt tgatacagca aaggcattat tcgatgtagc gtattatcaa gattttgtag
    30361 aaatccaaag tgagacagcc attcggcata tcgctagcca atacccgtat gatacattca
    30421 atgagaatga tttgacgctt cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag
    30481 agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt
    30541 tagcctatgc aacagaaatc gctagtgcca tgctccaacg tcaacaagca aaagccattt
    30601 tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat cactcaaatg gccttagagc
    30661 aaatcgaaga tggacaagaa atcaatttca ccgatgatcg gaaagtccaa ttgatcaata
    30721 atttacttgt ctcaatcatt acagacaaag gaacgcaacc tgttatcaat acaggcgaca
    30781 tgaaagaccg taagtgacat actttttgta agataggaag aaggagttta ggacggatta
    30841 ttgtcttgaa ctcttttttt agttgtttta aagaaaatga caaaattgaa agaagaatga
    30901 tagttgattc gaaggcagaa ttgttcgctt ttttctataa tcagtatata aagtaagaga
    30961 gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg ttttcatcgt tgtggtactt
    31021 gcaggggtga aaatcgcaga ttacgtagtt atgggtggag aagaatacta tgtacaaatt
    31081 acgacagatg gtacaaaaac agtttcaaaa tcagactctg gacaagaaac agtcaactat
    31141 agatatatcc tgcctggcta tacgaaagag ggtaaggaaa aagaactgga cttcaaggga
    31201 caattgactc gaccgcttcg taaaggagca tatttaaaaa tcacctggaa caaaaataaa
    31261 ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa
    31321 ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga agtacagaat gtaacaaaag
    31381 tatacggaaa aaacaatgag aaaaagacgc aagcattaag cggcatcagc ttcgatgtag
    31441 aaaaaggcga atttattggg atcatgggcg cttctggttc tggtaagtcg acacttttga
    31501 atattttatc cacattagat aaaccaacag atgggcatat ccgaatcaat caaaaagatg
    31561 tgaccacatt aaaaggaaat caactagcag attttcgtgc gaatgagatt ggctttattt
    31621 tccaagattt caatttatta gaaagcttga cagcacaaga aaatatcgct gttcctcttt
    31681 ctttacaagg cgtcagacca aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt
    31741 tatccatctc tcgtattttg gaaagctatc cttctgagat ttccggtgga caaaaacaac
    31801 gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat tctattaggg gatgagccta
    31861 caggtgcgct ggattccaaa agtgcccggg atcttttaga tacgatggac gaattgaata
    31921 caaaagatca tgtatcgatt cttcttgtga cgcatgatcc gttttctgca agttattgcc
    31981 aacgtatttt attcatcaag gatggcggta tccatcaaga agtgaaacgt ggagatcaaa
    32041 gccgtgattc cttctatcga gaaatactat ctattcttgg aaacttggaa cagtaggggg
    32101 agattgacta tgctttggaa attatcactt actgggatca agagccgttt aagagactac
    32161 ttggtgttat tctcaggatt agttatggca tctgctattt tttacatgtt tgaatcaatg
    32221 gcaagcaacg aagctttttt gaaaagtaat acgatcgttg cggctacagt tatcattttc
    32281 cgattcggtt ctgttttact agggatcatc acattcgttt atattttata tgcaaattcc
    32341 tttttgatga cgatgcggca aaaagactat gcaatgttta tgatgctggg agcaaaaggc
    32401 agaaaaatcg cacagatgat tttttctgaa acattcttag tagggatcgc agctacttta
    32461 gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc accgattatt agtggatcag
    32521 ttagatctca atatttcgca ttttacacct tttagtattc acgggttgtt gatcactgtt
    32581 ttgttttttg ctgttttgtt tttattagct gctgttgtca atgctttttc gattgtcaaa
    32641 aaaccgattt tagcattgat tcgtgcggat cagacaccaa caagaatcaa gcagcataaa
    32701 ttcttgtttt tccttgaagt agtactggga attgcttttc taggctgcgg ttactatatg
    32761 atgaaacacg tggggaccta tcaagtcttt ggtatagggg tagccttagt tacgatcgta
    32821 ttaggaactt atttcatttt ccattctgtg atcattttct ttttatcatt actaaagaaa
    32881 tcagaacaga tttcattgaa aaaattgaac aatttcacat tgtcacagtt aaactttcgt
    32941 attcgtgaat atacacaaat gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc
    33001 gctttgacgg taggattagg ttttaaaaat gaaattgcag acttgacaga aaacaattct
    33061 ggatatgatc tagtgatgaa taatcctcgg gcagaagatc agcaaaaaat caaagaactt
    33121 tcaccaacac tttctgcttc ttatactcaa aaagaagatg cgggaacgat ttactataat
    33181 gcaagtgaat ttgcggctca accattgatc atgataaaac atgaagatgg aacaccgccg
    33241 aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg aactgactgt acaagaggaa
    33301 cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca
    33361 gaatttgatc aactgaactt gccacaatcc actttacagc tagttcaggt aaaagacttc
    33421 caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa ataaaatgaa caatccatct
    33481 ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt atgaagtgta taatggtcta
    33541 ttctctggtt ttgaatttat gggattcttc ctaggtttag ctttcttgac catgcttgct
    33601 agctgtttga tgttcaagat cttgtcagga agcaagagtg acattgctcg ttatgaaatg
    33661 cttgaaaaga taggaacacg acgcgggttg cttaaacaat cgatccgtag agaagtaggc
    33721 gtgttattct tagcaccagg aatcttaggt gtagtccatg tcttattcgg attacaaatg
    33781 tttggtctat tcatgcaaaa tccatacaaa gatatttggg ttccgtttac gatattcttc
    33841 gtattgtatt tcatctacta tgtactgacg acatggttat atactggaat cgtattgaaa
    33901 aagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagct
    33961 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    34021 gttcggaaaa agaggttgtg acaatatttt tgtcacaacc tttttttcac ggtcttttat
    34081 tagaatatag taaatttttt ctcgttactc acggaataag ctgttaagaa aacggtttcc
    34141 atgtatgctg gaatcagtgc aaatgaaaga aaagagggtg caaggaacat ggaaatcaat
    34201 gaacaaaatt ttggcgaaaa ctatcacttg attacgatca cgaatcaaaa tgggacatct
    34261 ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc aaacaataat cgataatgaa
    34321 aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat atctagaaaa agacccttat
    34381 atcggtgctt caatcggacg tatggctgga cggattacga atggcacatt caaattagat
    34441 gatgaaacgt tcgaagtaaa aacagacccg gacactggtc attggtttac atggtggtgc
    34501 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    34561 ggtcattttc tatcagacta gcccagatat ggaaaacgga tttcctggaa ctttagatgt
    34621 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    34681 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    34741 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    34801 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    34861 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    34921 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    34981 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    35041 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    35101 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    35161 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    35221 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    35281 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    35341 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    35401 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    35461 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    35521 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    35581 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    35641 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    35701 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    35761 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    35821 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    35881 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    35941 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    36001 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    36061 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    36121 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    36181 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    36241 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    36301 cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    36361 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    36421 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    36481 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    36541 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    36601 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    36661 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    36721 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    36781 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    36841 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    36901 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    36961 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    37021 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    37081 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    37141 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    37201 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    37261 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    37321 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    37381 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    37441 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    37501 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    37561 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    37621 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    37681 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    37741 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    37801 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    37861 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    37921 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    37981 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    38041 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    38101 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    38161 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    38221 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    38281 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    38341 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    38401 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    38461 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    38521 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    38581 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    38641 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    38701 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    38761 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    38821 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    38881 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    38941 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    39001 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    39061 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    39121 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    39181 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    39241 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    39301 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    39361 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    39421 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    39481 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    39541 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    39601 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    39661 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    39721 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    39781 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    39841 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    39901 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    39961 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    40021 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    40081 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    40141 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    40201 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    40261 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    40321 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    40381 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    40441 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    40501 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    40561 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    40621 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    40681 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    40741 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    40801 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    40861 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    40921 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    40981 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    41041 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    41101 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    41161 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    41221 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    41281 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    41341 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    41401 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    41461 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    41521 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    41581 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    41641 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacatgtg ccagtactta
    41701 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    41761 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    41821 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    41881 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    41941 aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca tattatctgt
    42001 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    42061 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    42121 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    42181 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    42241 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    42301 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    42361 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    42421 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    42481 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    42541 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    42601 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    42661 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    42721 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    42781 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    42841 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    42901 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    42961 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    43021 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    43081 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    43141 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    43201 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    43261 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    43321 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    43381 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    43441 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    43501 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    43561 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    43621 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    43681 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    43741 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    43801 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    43861 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    43921 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    43981 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    44041 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    44101 ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    44161 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    44221 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    44281 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    44341 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    44401 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    44461 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    44521 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    44581 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    44641 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    44701 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    44761 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    44821 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    44881 aagattctct aagttctgtt tcagccaaac aatttggaac gatcattgac tatcaagcaa
    44941 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    45001 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    45061 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    45121 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    45181 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    45241 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    45301 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    45361 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    45421 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    45481 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    45541 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    45601 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    45661 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    45721 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    45781 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    45841 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    45901 aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa acaaacggca
    45961 aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
    46021 ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat actcaaataa
    46081 aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa agcttttgaa
    46141 caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat agacaaatac
    46201 atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata aagataaatt
    46261 ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata taagttgtgg
    46321 taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac aaagtcattt
    46381 accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga gatttaaaag
    46441 gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt tggctaaacc
    46501 ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac acagccatcg
    46561 atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca aagaaatacc
    46621 ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat gaatggttca
    46681 aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga gaagagccaa
    46741 cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat acggggaaat
    46801 attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac caagcagtaa
    46861 gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa ggattccgtt
    46921 tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa gaaaatgatg
    46981 gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa ttgaaccaag
    47041 caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct acgacgatcg
    47101 aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc tttaatttcc
    47161 atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca tttgattttg
    47221 cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt aacggctgga
    47281 atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt gtagctatcg
    47341 atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg aaccgtggga
    47401 cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat tcatcgattt
    47461 ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa caaggattca
    47521 cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct cgaacaccca
    47581 tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg ttaaaagttg
    47641 gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat tctattttgt
    47701 cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca caaggagatt
    47761 atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa tttgaaggac
    47821 agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc atcccagaag
    47881 gattcacaga agccaatatt cttatcgata attattccga tactgttctt catcaaagtc
    47941 tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa aacacaaaac
    48001 gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta aaataatctt
    48061 cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta ttgtagcgtt
    48121 gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag ataggaatac
    48181 caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg attaagatcg
    48241 taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag tcaccgtgga
    48301 gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta gagtcttgga
    48361 atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga atgatacata
    48421 acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt aagaaacaaa
    48481 cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga aatgaaccca
    48541 attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct ggtgggacaa
    48601 tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa gcgacaattg
    48661 gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg ctgatccgtt
    48721 gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc acgtcgtcaa
    48781 tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa aattgtcgga
    48841 gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc tttgcctgtt
    48901 cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa ataatcacat
    48961 aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg atgactggtt
    49021 ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta gcgataatga
    49081 ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc agcgcaagta
    49141 agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg atcaaacgat
    49201 tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact tcattataga
    49261 caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg tcaatcattc
    49321 atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt aaccatatga
    49381 taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg aatcggaaca
    49441 ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat aggtatattg
    49501 gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa agccgaggac
    49561 agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt tacaaaagca
    49621 atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga ttcccacgat
    49681 tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt tgtttataat
    49741 gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga aggcaatgtc
    49801 catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga aggacatgat
    49861 catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca tgtatggcta
    49921 gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat tgaagcttac
    49981 ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa acttcaagca
    50041 ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt tgtcacgcaa
    50101 catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc gattgctggt
    50161 ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca ctttgttaaa
    50221 gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat tgccagaacg
    50281 ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg attgacgaat
    50341 gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct cgaagcgttg
    50401 aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa aaaagaaaag
    50461 agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct ttcagattgg
    50521 gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga tcctgttttt
    50581 gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga atattatacg
    50641 aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat ggcatttacg
    50701 tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga aatcttgacc
    50761 tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa ggaaagtggc
    50821 gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc cgaacatttt
    50881 catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga gaattggcca
    50941 acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct tttgcactga
    51001 taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc
    51061 ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg
    51121 tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata
    51181 acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc
    51241 tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc
    51301 gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct
    51361 attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg
    51421 atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt
    51481 tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt
    51541 tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg
    51601 tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca
    51661 ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc
    51721 atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa
    51781 atataaatgg gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa
    51841 aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca
    51901 tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt
    51961 tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg
    52021 gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct
    52081 tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta
    52141 tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc
    52201 atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt
    52261 agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa
    52321 gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt
    52381 tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa
    52441 tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt
    52501 ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta
    52561 ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag
    52621 tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac
    52681 caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag
    52741 acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa
    52801 aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaagaaag
    52861 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    52921 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    52981 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    53041 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    53101 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    53161 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    53221 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    53281 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    53341 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    53401 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    53461 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    53521 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    53581 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    53641 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    53701 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    53761 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    53821 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    53881 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    53941 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    54001 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    54061 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    54121 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    54181 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    54241 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    54301 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    54361 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    54421 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    54481 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    54541 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    54601 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    54661 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    54721 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    54781 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    54841 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    54901 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    54961 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    55021 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    55081 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    55141 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    55201 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    55261 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    55321 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    55381 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    55441 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    55501 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    55561 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    55621 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    55681 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    55741 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    55801 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    55861 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    55921 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    55981 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    56041 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    56101 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    56161 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    56221 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    56281 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    56341 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    56401 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    56461 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    56521 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    56581 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    56641 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    56701 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    56761 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    56821 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    56881 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    56941 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    57001 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    57061 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    57121 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    57181 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    57241 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    57301 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    57361 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    57421 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    57481 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    57541 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    57601 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    57661 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    57721 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    57781 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    57841 aatgcgatca atacttttct cctaaatatc aaaatatatc cgttgcagat tatattttgt
    57901 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    57961 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    58021 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    58081 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    58141 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    58201 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    58261 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    58321 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    58381 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    58441 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    58501 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    58561 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    58621 ggaggtgtga caagaatttt gtcacacctc ccttatttat tttgaaaatg agaatactat
    58681 tatcaaaata gagaatatat agaaaaattc tcattattca aacgtaaata atcagttatt
    58741 ttgatttttt ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa
    58801 ttggattata attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta
    58861 cttcctgtag aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga
    58921 atatttttgc cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag
    58981 gaggagttca agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt
    59041 ttaacgaatt aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga
    59101 gacgacctat tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc
    59161 ggtcatttgt ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca
    59221 ataacatctt aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg
    59281 cactgtgtaa caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa
    59341 tttgttgact tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt
    59401 gaagaagtgc aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg
    59461 attgaccttt cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat
    59521 ctcctcagac gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag
    59581 ttgatggaac agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc
    59641 atttcataca gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt
    59701 gactttattt ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg
    59761 tggggatata gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct
    59821 gttgttttaa gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg
    59881 acatgttaga aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc
    59941 ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg
    60001 ataaatgagt tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt
    60061 gacaattact tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg
    60121 gg
//
LOCUS       DAFFQY010000004        56096 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFFQY010000004 DAFFQY010000000
VERSION     DAFFQY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.004"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(854..1384)
                     /locus_tag="LRB02_000200"
     CDS             complement(854..1384)
                     /locus_tag="LRB02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL4658442.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1381..1998)
                     /locus_tag="LRB02_000201"
     CDS             complement(1381..1998)
                     /locus_tag="LRB02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4658443.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2580..2801)
                     /locus_tag="LRB02_000202"
     CDS             complement(2580..2801)
                     /locus_tag="LRB02_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL4658444.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(2979..4511)
                     /locus_tag="LRB02_000203"
     CDS             complement(2979..4511)
                     /locus_tag="LRB02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4658445.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4647..5732)
                     /locus_tag="LRB02_000204"
     CDS             complement(4647..5732)
                     /locus_tag="LRB02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4658446.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5747..6466)
                     /locus_tag="LRB02_000205"
     CDS             complement(5747..6466)
                     /locus_tag="LRB02_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL4658447.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6463..7773)
                     /locus_tag="LRB02_000206"
     CDS             complement(6463..7773)
                     /locus_tag="LRB02_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL4658448.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(7800..8390)
                     /locus_tag="LRB02_000207"
     CDS             complement(7800..8390)
                     /locus_tag="LRB02_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL4658449.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8708..9769)
                     /locus_tag="LRB02_000208"
     CDS             complement(8708..9769)
                     /locus_tag="LRB02_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4658450.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10337..11404)
                     /locus_tag="LRB02_000209"
     CDS             complement(10337..11404)
                     /locus_tag="LRB02_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4658451.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11424..11903)
                     /locus_tag="LRB02_000210"
     CDS             complement(11424..11903)
                     /locus_tag="LRB02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL4658452.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(11920..12382)
                     /locus_tag="LRB02_000211"
                     /pseudo
     CDS             complement(11920..12382)
                     /locus_tag="LRB02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            complement(12396..13646)
                     /locus_tag="LRB02_000212"
     CDS             complement(12396..13646)
                     /locus_tag="LRB02_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL4658453.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14024..15475)
                     /locus_tag="LRB02_000213"
     CDS             complement(14024..15475)
                     /locus_tag="LRB02_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL4658454.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15796..16560)
                     /locus_tag="LRB02_000214"
     CDS             complement(15796..16560)
                     /locus_tag="LRB02_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL4658455.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16588..17286)
                     /locus_tag="LRB02_000215"
     CDS             complement(16588..17286)
                     /locus_tag="LRB02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL4658456.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17298..18077)
                     /locus_tag="LRB02_000216"
     CDS             complement(17298..18077)
                     /locus_tag="LRB02_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL4658457.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18093..19037)
                     /locus_tag="LRB02_000217"
     CDS             complement(18093..19037)
                     /locus_tag="LRB02_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4658458.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19083..19862)
                     /locus_tag="LRB02_000218"
     CDS             complement(19083..19862)
                     /locus_tag="LRB02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658459.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20242..22317)
                     /locus_tag="LRB02_000219"
     CDS             complement(20242..22317)
                     /locus_tag="LRB02_000219"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL4658460.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22319..23236)
                     /gene="glyQ"
                     /locus_tag="LRB02_000220"
     CDS             complement(22319..23236)
                     /gene="glyQ"
                     /locus_tag="LRB02_000220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL4658461.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23622..24440)
                     /gene="recO"
                     /locus_tag="LRB02_000221"
     CDS             complement(23622..24440)
                     /gene="recO"
                     /locus_tag="LRB02_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL4658462.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24583..25482)
                     /gene="era"
                     /locus_tag="LRB02_000222"
     CDS             complement(24583..25482)
                     /gene="era"
                     /locus_tag="LRB02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL4658463.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25497..25892)
                     /locus_tag="LRB02_000223"
     CDS             complement(25497..25892)
                     /locus_tag="LRB02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL4658464.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(25876..26352)
                     /gene="ybeY"
                     /locus_tag="LRB02_000224"
     CDS             complement(25876..26352)
                     /gene="ybeY"
                     /locus_tag="LRB02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL4658465.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26381..28573)
                     /locus_tag="LRB02_000225"
     CDS             complement(26381..28573)
                     /locus_tag="LRB02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL4658466.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28597..29568)
                     /locus_tag="LRB02_000226"
     CDS             complement(28597..29568)
                     /locus_tag="LRB02_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL4658467.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30273..31652
                     /gene="brnQ"
                     /locus_tag="LRB02_000227"
     CDS             30273..31652
                     /gene="brnQ"
                     /locus_tag="LRB02_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL4658468.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(31808..32254)
                     /locus_tag="LRB02_000228"
     CDS             complement(31808..32254)
                     /locus_tag="LRB02_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL4658469.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32281..32457)
                     /locus_tag="LRB02_000229"
     CDS             complement(32281..32457)
                     /locus_tag="LRB02_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL4658470.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32619..33047)
                     /locus_tag="LRB02_000230"
     CDS             complement(32619..33047)
                     /locus_tag="LRB02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4658471.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33139..33999
                     /locus_tag="LRB02_000231"
     CDS             33139..33999
                     /locus_tag="LRB02_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL4658472.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34093..34443)
                     /locus_tag="LRB02_000232"
     CDS             complement(34093..34443)
                     /locus_tag="LRB02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL4658473.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(34459..36186)
                     /locus_tag="LRB02_000233"
     CDS             complement(34459..36186)
                     /locus_tag="LRB02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL4658474.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36399..37325)
                     /gene="trxB"
                     /locus_tag="LRB02_000234"
     CDS             complement(36399..37325)
                     /gene="trxB"
                     /locus_tag="LRB02_000234"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL4658475.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37470..38906)
                     /locus_tag="LRB02_000235"
     CDS             complement(37470..38906)
                     /locus_tag="LRB02_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4658476.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(38903..40306)
                     /locus_tag="LRB02_000236"
     CDS             complement(38903..40306)
                     /locus_tag="LRB02_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4658477.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40536..41438
                     /locus_tag="LRB02_000237"
     CDS             40536..41438
                     /locus_tag="LRB02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4658478.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41479..43365)
                     /locus_tag="LRB02_000238"
     CDS             complement(41479..43365)
                     /locus_tag="LRB02_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4658479.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43462..44314)
                     /locus_tag="LRB02_000239"
                     /pseudo
     CDS             complement(43462..44314)
                     /locus_tag="LRB02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44649..46433)
                     /locus_tag="LRB02_000240"
     CDS             complement(44649..46433)
                     /locus_tag="LRB02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658480.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46433..48184)
                     /locus_tag="LRB02_000241"
     CDS             complement(46433..48184)
                     /locus_tag="LRB02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4658481.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48326..48550)
                     /locus_tag="LRB02_000242"
     CDS             complement(48326..48550)
                     /locus_tag="LRB02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL4658482.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            48801..50387
                     /locus_tag="LRB02_000243"
     CDS             48801..50387
                     /locus_tag="LRB02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL4658483.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50453..52228)
                     /locus_tag="LRB02_000244"
     CDS             complement(50453..52228)
                     /locus_tag="LRB02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL4658484.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52439..53341)
                     /locus_tag="LRB02_000245"
     CDS             complement(52439..53341)
                     /locus_tag="LRB02_000245"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL4658485.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53620..55965
                     /locus_tag="LRB02_000246"
     CDS             53620..55965
                     /locus_tag="LRB02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL4658486.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16609 a        11682 c         9086 g        18719 t
ORIGIN      
        1 tttaaaatta acattgattc tcctatgaaa atatctttta cctttctcct gtgaaaagga
       61 aatttccttt tttatagtgt tattgatata atcagaatat tcatcaaact ctgtatctgg
      121 agttaaaaat ctacagtaac atctataagc tttgctagta atcttcttgg gttctctctc
      181 cattgtcaag caacatcaaa acacctactt actattcgat tcttttctgc caaaaacgag
      241 tcagcatcac tcacaactta tgtgttgctg actccgacta gtcctttgtt caatatcaac
      301 ttattaaaca gatataatat ttttctttat ttcgtatact ccaagacttc catctttctt
      361 tcatcttatc tctgaaaaaa acacagcaaa gtgcccttaa taaatataca ttagaatttg
      421 caaatttttt catcttaaga tatatcctat tcatattctt tacaatccta atgggtgatt
      481 acaaatctat ttagttcaag ttctacatta taaacacctt tcttgatagt atcatctgct
      541 gcacaagcga tgtcgatcca cttattaggg gatttacgtg ctcaaaagtg gctattcctt
      601 ttgggaagtc catccataat aatggtccat ggattttttt agggtcttgt cgttcaaatt
      661 tctcaaaaac tttattatac ttccatagtt ttataattat ttggtttcac tttggctcgt
      721 tcaaacacca acttgtattt cttttcaacg ttcgaactgg agccccccct ataatctcat
      781 tattattttc atgacttttt actaccgtgg aattacaagc aacaacaata ttatctgcta
      841 aatgaacacc tttctatatc acgctttttg ctccaatcca acaatgttta cctataacta
      901 ttttattatt tttattcaaa atcctatgag tattcaattc aaaaatcgta tgtgaatcgg
      961 tatccataaa taatgtatcc cacgatactg tacagccatc tttgaattga atttccttaa
     1021 agcaaattat actcattttt cctgtcgaat taaactttga cccaatatct aaaactgcat
     1081 ttttccttat gtcaatatga gccccttgtg cgataaacgc tggcccttgt attcttagta
     1141 ttccttcaat acttataatc gtttcttcat ttcccgcagt atttaatact ggaaagccca
     1201 aataaactat ttttccagat tgtaatacta tctcaatttt ccctctcgat ttgcgtgtaa
     1261 tccgtacttt acgatgcacc aaaataggta atctccacgc atctgaaata tgaagaaatt
     1321 tataattgaa gtatatcgtt ttaggtaaat ttaatatatt ctttattatc aatccgagtc
     1381 tcatctttca ccatctcttt ttgtaatcaa aattagttta taaaggatta cacccaatat
     1441 accgcccaac gtattatata caaaatccga aatttgaaac gttcctagcc tcaaaaaaag
     1501 ttgtaaaaat tcaattattg ctgaaaaata aaatgcaatt tttcctccat atactattga
     1561 ggaccatatt ttgggtttct ttatattttc atatttaata gacattacca tataaacata
     1621 tggaataaaa agaataatat tttctaaaca cgctgtagat aactcttttg ttccagttgt
     1681 tgtattatac ttataaatcc accagtctcc cataacatca gataaaggat ttaaccaaat
     1741 tgttcgattg attaatgttg taaataaaat catcgcagta tagaatatca aagcacaaac
     1801 ttttctatat tttctatcct tctttaaatg aagataaagt tccattagta tacgtttata
     1861 gccctcacct gcagcaatgc catctgttgc atacataaaa tagaccagtc ctaaaattgt
     1921 caataaaaca gcaaacccaa agggttcata taatttatat aatatatctg taataatttc
     1981 tgcaatcact attatcataa ttatttctcc aatatcaagt atacacttat cgcttccaat
     2041 tacacatgat catattagag tgtattatca cattctctaa tattcaattt atttttccta
     2101 aaatattcga aataatcgtc ttgataacga gtaagtattt ttatacgatt gataacatag
     2161 taacaatatt tttaattata gtaaaaaatc atctgccttt ttcatccacc taatcttatt
     2221 tttcatttta ttcaacatcc ttaaataaac ttccgttcct aagctctata atatcttctt
     2281 aaaaaataat attatggtgt cgttttcaaa ctcgtaatct tttacattgg aaaaatacat
     2341 acttttctca atgtagcaac tcctatctca cagtattcaa aaattcttca atattatgaa
     2401 atacttgtgc atgttcttta aataccttgt cagtctgttt ataatcccct actaaatttt
     2461 gcattgctcc caactcttcg acaaattgtc atctccatct gtaaacagcc ctaaaaactc
     2521 atataatgct ttttcatcaa tatttttccc atattattct cggtatagtt tcacaagttt
     2581 tacggattct tcatattgtt tatcttttta taatattcat ctagggacat aattactttt
     2641 gctagatttc ctgcaacaac acaatcgtct gatacgtcct tgtttactaa actatttgca
     2701 cctataacta cattatttcc tatatgtaca ccctttagta ttctagactt cattcctata
     2761 aaaacattat tacctatttt gatcctctcc gatgatccca aagcttctcc atactacttt
     2821 taaaaccgat caatcataaa catgagtcaa aattgtaact ccttcatatc atctccaatt
     2881 tcaacaagag tcttcgttgg aacaaaaact atacatcgtt cgcctattcg gcagcctaaa
     2941 aattgtagtt actttatata tcattctgat gtctcttctc atctttttac caataccgac
     3001 gtaagaactt tcaaaatata cttaaacttc ttattacgga tattaaacag aagaataact
     3061 aaaatcgata tgacaaccga tataactaaa tttatcacta aacttatcac tttgaaattt
     3121 ccgatattaa catacgaaca agcaaaataa cttaaataag aagcaacaaa agctattaca
     3181 cacaaactta tgagcttcca aacatattta cctgccgatt caaatttaaa tatattttgg
     3241 aaaacattat ataataacca gggtaaatca accaacaata ctgaaacaat ggttgccgcc
     3301 aaaactccat ataatccgaa ccaattaacg aggatgatat ttaatacaat gtttatcatt
     3361 gctgtaatta atggtctaaa cctgtctgcg taccatattc cagcagcatc tttgtataac
     3421 ccaattaact gatcaatttc atataagaaa aaatgaacta tcataaatat aacaaatgaa
     3481 atactcataa gattatcact tcccacccag ataatcatga acggctgaaa taatgtaata
     3541 aagcatgcac aactaattgc cagaaaccaa cctataattg ttgtaatatc acaaaaatct
     3601 atataatttt tttcttgact ttctacaata agactgttac ctatacctgc tatacatgat
     3661 tcataaatta ttgcaaatat actcattatg gcagaaataa taaagtaata attctgataa
     3721 atcgcaagta ctgaaagacc taagaaagat gaaataataa ttgaatctga agatcgtaat
     3781 attgtaccgc ctaccttgtt tgtaactaat ccttttactt tttttataat atcgtcaata
     3841 gtttctttct ctaactcacc aacaggagta taatcaggat acatcttttg agaaacaaca
     3901 gcacacatta agttaagaac tatttgtccc aaaatagaag ccaataaata caaatagtaa
     3961 ttttttaaga aaatcagaac atatgcctgc attacaaatt ggaatgtaaa agtaattagc
     4021 atagacacgt taattacatc atttctttga tgtgcatata ttagactctt tctatatgaa
     4081 aataaccaat acgacaaaac agtatttatc aaatacatta aatacaaaac tgtcatattt
     4141 aaatttccag gaatcgaacc tttaactaaa aatctcaaaa aaggaatgca taacaatcct
     4201 aatgtaaaca ctatccatcc aataattgaa aagtactttt tatatgcgtt cagtaaagca
     4261 caaatctctt ttctattgtc ttctgctata ggtttataca tacttacagt tagtgcagca
     4321 cctattccca attccgctaa atttaacatt tgaaatatag atgtgaacag gttgttcaat
     4381 ccagcatact ccattcccat ctgataaatc ataagtgtac gcataaaaaa gggaatgata
     4441 atctgatata acttaaaaat tgttccagta attatattcc ttttagcatt ttgtttacga
     4501 ttaattatca tatcatatgg ctccaaactt tctatttttt attgttacct aatactatat
     4561 aatttctaaa atcttgaaat gtaataggtt tcttctagct ctttgattaa ttacgctaat
     4621 tttcatgttt ggacaaccac aaaaaactat acattttcaa tcaaagcaac ccattgtttt
     4681 gtaatttctt tcaaattaaa tttttgtaca ttattatatg cgttcagtga cattctcatt
     4741 gctaaatcag tattcttagt aaacattatc atgctcttag ctaattggtc tatattctta
     4801 tcttcaacca acatcccatt aaaattatga tgtaccaaat ctctcggacc agttttacaa
     4861 tcataagaaa taactggtaa tccataagac tgtgcttcta atataaccat aggaaatcct
     4921 tcataaacag aactaagcac ataacaagat gcagatttat aatattcctc cacatggtct
     4981 gaatgccctg ctatagttac tctttgcaga tttaaatctt ttatctgctg gcgaagatct
     5041 ttttctaatt caccttttcc tacaatgata agatcccaat catctacttt tttttctaca
     5101 attttccaag cacttatcat gtcagcaaaa tttttctctt ccactaatcg ccctactgca
     5161 agaaatctct ttttatcata atgatattcg tactcgatat tagatataca cggatttggc
     5221 atagttataa caaaatcttc tgataaacta tattcatcaa tatgtttttg acgatcttct
     5281 tctgtgagaa ccacaagcct ctttgcatat ttttttatca attttttagc cttgaatctc
     5341 ttcttttctt tcagcatttc cattgtccag taattaaaat gttcccaaga aatcaatttg
     5401 cactttgttc ctttaattgc ccttgtagta tacaaagaca acaaagtatc aatatcaatt
     5461 actatatcaa attcattttt gcatatatac ttgtgcaatt tccaaaccgg atataaataa
     5521 gttctatata attttccctc cgttttagga tttaacatat aatcaacctt aatcgtctta
     5581 tctaacccaa aataaggctc tccactattc caaaagctta aaatagtgac atcataacct
     5641 aaatcagaca agctattggc aataatactc cctactcttt cagttccccc agaacgagaa
     5701 attacaccac taatgaaaca aattttcttc aaagtacttt tctcctttat aagtgtaaaa
     5761 attttttcag tgcaagtaac ctgttaaatt ttttttgtcc aattgttgat tttaatctat
     5821 aaacaaattt ttgtttaggt gttttccaag atgcattata ttgatgaatg cttattgtat
     5881 tactagtttt ataaattttt ccagtatcat aatccttggc agcaaaataa tccgtaggat
     5941 agatatgcag gccatttatt atttgttcgc tgttatcgcg aaccaatcca atagattcaa
     6001 gataatctgt aatataatac ggactcggtt tcaagttcaa tgtaccatca ttttgataaa
     6061 aacttaatga atcgtaaata tcccttaaac ctttaataac agaatgtcct tcttctgcac
     6121 caaaaccaat accgccatta acccagattt ccccttcaaa tcccatatag gcatgatttt
     6181 cacgaagtat atctaacgac ttaattagct caacatctgt atctaaataa aatccaccat
     6241 gattataaat aatatcaaat cttgcataat ctggaacaaa accccattta cgttctttat
     6301 atgcatcggc catataacgg ttcttattta aatcataatt tgattcattc cattcaataa
     6361 tctcataatc agggcaatac tttttccagc tttctatgca ctttctatct tttttcggaa
     6421 gtggatttcc cccgaaccaa cagtaatgta tcttcttagg aatcataata tctctccttt
     6481 tgtatttttt tcactataat aatgctcttt ccttttcaca acttcaggca tggctaaaat
     6541 catataaatc agtggccccg ttgtttcacc cctaagtgtt gtgttataca caaaaaagaa
     6601 taccgacata attgcaataa ctttaacaag acttgcatct ttatccttca taaaagatct
     6661 ttttgcattg aaaaatgtct ttattataaa aaataggaat atcgaaactc ctaatattcc
     6721 aacttctata aataaccaaa tatgatgaaa atactgataa tgaagattac caaacgattt
     6781 ataaaactca ctgacaaaaa aattggtaaa ctctgcattg ccaatgccta ctcccattag
     6841 gactaatcta aaattattat taaatatttc atctactact ctagagatac cagttattcg
     6901 atttaaatca ccaacaccat catatccaga atctcttgac gcatattcta aaagaccttg
     6961 aattgtgaaa ttatcagcat agctttgtcc attatcgttc aatgtaataa atattgttat
     7021 aaaaaaggat aatactatcg acacaagtat tacgcatgat aatatcttga tcactacttt
     7081 aaaatcaact tttaaagtga atactattgc aacaaatatt ataaataatt caggaataaa
     7141 aactttcaat tcagctaatg cagacataaa aagtgctgca caaacacata aaataaaatg
     7201 gatgaaattt ttttctccct tgaaatatcc gattgtaaca acagtaactg tcacacacaa
     7261 aattaaattc aatggcatat tacaacctga ataattccaa aaaactccac ctatcatatc
     7321 tcctacaatt gttccagttg gataggtgac catgaatctt tcaattaaac aaagtggcag
     7381 tgaaatccag aataattttt caattatagc gaatacttta tagtaatctt taattgttaa
     7441 atatgttgca caatttatat atactatgaa catacgagtg ttatttcgta tcccccagat
     7501 aatcaatcct atattttcta aattaataac tgagctacaa atagaaacta gtaaaaacaa
     7561 aactatcagc atctcatttt tgctagcata tattcctttt ctgcgtctaa agctaagtgc
     7621 atttaataac acaaagatat taattatatc tcccaaatat aaaattgctc ttggggcgtt
     7681 aaaagtatat acaagaacta tggagattgc aaaaaatgcc aacgtgaaca atgtccaatc
     7741 tgctcctgac catcttttta ttgaaatacg catcgttatt caccttcttt cgctaaaaat
     7801 tattctaaaa atacttttgt taaattaaca taccctatta tttttgccgg tacacctgca
     7861 agaattgcac cattctctag acaatctttg ataacaatag ctcctgccgc aacaactaca
     7921 ttgttagcta tttttacatt accaataatc tttgcaccca cgcatacacg tacattattt
     7981 ccaattacag gacacttagg atcgattcca tcatttccaa tacagttatt cccatataag
     8041 taacaatttt ttcctattat ggcgttacca ttaataacaa ttccttgtgt atggtaaaat
     8101 cttacacctt catcaaatac attttcacca cactctatac cacattttct tcccaaacta
     8161 ttcattcttc tgcaagacac aatatacata atcgcataaa tgatattttt ctttctcttt
     8221 gaataataat aagatgtaat tctcattcga cgagtatacc gccaagcgtt gtagtccgga
     8281 tgtgatttca ttatttttag taattttgtg tgttgtcctt tagattctat atatagattc
     8341 ttttccatac gaagaaccgc ttttaaatat tctttactgc taatttccat tcatatctcc
     8401 tattaattca atttatactt aacaatttta ttcggatttc ttgcaacaag agcatcattc
     8461 aatgctactg aatctacaac gatggatatg ttatcttcat cgttgattcc accggataca
     8521 atattactta taaaaatcgt aacatttttt ccaaaagaag ggatgtccgt gatgtataat
     8581 gcccattttc cgatatttcc agttatttaa acctaacgaa tttttacaat agtacaagta
     8641 tcacaatcat taatatataa ctggaatata aatctccaaa aattcactat agtacagttt
     8701 tttatgatta aaatctccta tttctaccca ttccgcttaa atatgctaat gttttaaccc
     8761 caataatgga ttggtttagt aaaaaatagt taaatattcg ttgtttaatt ggattttgtt
     8821 tatatgggta tgagtgaata caggcaatca actcatcgtt cataagtatc ttagacaaat
     8881 aggagtttaa atcaaattct ttattaaaac tagcatatct aactgccttt tgaatacaag
     8941 tacgacagcg tcccaacaaa agtctccttg ttttgtttac aaatatctca ttttctaata
     9001 tatagcttaa atcgccttca atttttttaa ctaattcaat attacaatcg aactcattat
     9061 ctctaaaatt atgtgtcaat gaattattat tattacatct ataataatag atacaatctt
     9121 taataaaact tgttattttt gaatgttgca aatatgaaat atggaatatg atatcttcag
     9181 atatataatc cctttctgac ggaaacttga tattgtgctg ttgaattatc tcagatgaat
     9241 acagcgcatg ccaaactgac attggcaata taaaatcgac tttataactc ggctcagtgc
     9301 caatcattcc aagcaacact ttttcaatta tagcgttatt tgtaaatatc gtactatcat
     9361 atcttgctgg ctttgaaata atattattat catcataata ttcataatat ccagcataac
     9421 atgtgtctgc attactcctt tttataccat ttaacattgc ttcaatataa ttagctgcaa
     9481 caaaatcatc agaatcaata aaagcaacat attttccttt tacatgttca agtcctgtat
     9541 ttcttgccaa tcccaagcca gcattatttt tatgaataat acgaatcctt gaatccttta
     9601 atgcatagtc ttcacatatt ttattagatg aatccttaga tccatcatcc acaattataa
     9661 tatctaggtt cttatatgtt tgattacaca ctgattccaa acaacttcct acatactttt
     9721 caacattata tacaggtatg attacactaa ttagatcact cgaattcatc cttctatcct
     9781 ttcactcacg ttttctcaat tagtcaatga cggcatcaac gattttctta aattctctga
     9841 aaaaaataga ctctgatctt gatgatgtga atgacatact atcttcttct agaaaaatta
     9901 tttcctttaa attatttatt aaatcaataa tatgatcctc gtaagtatgc tttcccttgg
     9961 aataatgaaa atatactttt gtattacaca atattgcaat tttatttctt aatatgatta
    10021 tttcactcat tcattactgc cacatataca caacttataa ccaccccctg caaagtttag
    10081 ggtaagttat cagataatat cttcctcttc atcatccaga atacaaaatc tatttattta
    10141 tagaaatatc attaatgttt ttatataatg taccgtgctt gtactcttgc tctattacaa
    10201 gcactaaaac actactattc atttgaattc ttacacggat aaaaatttac atcgtctatt
    10261 tcaatatttt ttctctctat tttattcact taataagttc attacataat cgaaacattt
    10321 tttattttcc ttgtcatcac tccttgctcc ctttttgata cagatttaaa attctatcaa
    10381 agaaatttcc atcgttaaat ttcgttattg ccaaatcctt cgaacaacgt gaaaatttta
    10441 cgtatttatc tttatcattg atttttagta ttgcagatgt caacgctgat acgtcaccag
    10501 gttgaaacaa atatccattt tctccatcac gtactgctga agaaatatct cctacatctg
    10561 tagcaattac aggcattcca taagaaatcg cctctagaat agatataggc aatccctcgt
    10621 tataggacgg taaaatcatc acatttgatc ttctatagca ctcctccttt ttttctccca
    10681 caacccaacc tgtaaattca aaatacgatt gcaaattata ttcttctatc aattctttga
    10741 gcgatatttc ttcatcacct gctccagcaa tctcaaaaat aaactttttt tctggatatt
    10801 gttctgcaat gtttttaact gcttctacca aatcgtgtac accttttcgc tttatcaaaa
    10861 ctcccataaa cagaaatcgg cacatttttg aattccactt tatggtatat tctggaacta
    10921 caatagaatt ttctacaaca acagttttac aatttggctc aatttcttta attatcttct
    10981 tccatttttc tccaagtaca atcgtggaat cagactctct taacagcaag cgaatctgat
    11041 tttgtttttt tacagaaaca ctatcatacc attttttaaa ttccgatcca tgaagatgga
    11101 tgattacagg aatatttaat aatctaaacc acttattcag cacatatttc ctactaaaac
    11161 tccctctgta cgacatgtgc atatgaataa tattaggttt actcaataaa gagataccta
    11221 taattttagc aaatgcatac ataaaatata aagatttaaa tattgcattt ccacccttat
    11281 aactcggtat aaaagtcatc tcgatattct ttttttccca attaaaattg agcatttgtg
    11341 taattatact tgtaattcca ccttttaccg agctgtcatt accaatcata agtactcttt
    11401 gcataacgct ttactcctta tatttatatt ttttctatta atacctctat cgaattaata
    11461 atcgtttccg tgttagatac atacttgtta aacatccgaa ctcttgtttc tttaacggca
    11521 tcttctaaat tttctaattc ataacaagga tagataatat tcatctcatc aaattgtcta
    11581 attaactgca gttggtgatc atcgacatgc tcaccatact tagccaatcg aggaacagca
    11641 atgacccttt tacctttttt tacggcccca attatagcac ctgtacctcc atgagtaatc
    11701 accatttctg ctttttgcat agtttcagta aactcatccc tatttagaaa tgctttatag
    11761 gaataattta gtggtttata atcactataa ccactctgtg caaatgtact ttcgattatt
    11821 actccttttt caattaaatc atcaatagct tttaacaatc tatcaaattg aaatttttgt
    11881 gaacctaatg ttataaatat catttttttc tcctattatt taatatatcc cacccaaata
    11941 aatagcatta ggataaaatt gtagcatagt ttcccactga acataaaatt gatctgcaaa
    12001 atgatacatc aattttcctg tctgagtagg gctggttact tttgcaaacg attcaatata
    12061 aatcagtttt tttcccatca tttttgttat taagcagata gggatcatag ctaaaacacc
    12121 tgtacaaatc acgagatccg gtttttcctt tatatatata ataattgatt tgaataaatt
    12181 aataagtata gtacacaaaa aaaacttttc ttttctattt acttgtctca tgtagtacgt
    12241 tttttcgcct tttgactcag ctgaatattc tgtctgttct gttataacaa aactctcata
    12301 cttctccatc agtggtttta acatcattaa ctgttcataa tgccccccca gaggaagccg
    12361 caaaacatat ctttttgttc atattaacct ctctatcagt cacgctgaaa aatatctcta
    12421 gtgtaaactt tttctttaat atcatccaag caggtatcat atcgattggc aataatagaa
    12481 tcacacatcg ccttaaactg ctctaaatcg ttgataacct tacttccgaa aaaagtctct
    12541 ccatctttca gtgcaggctc ataaatcacc acagtcgcac ctttagcttt tacgcgcttc
    12601 ataactccct ggattgaact ctgacgaaaa ttatcactgt tgctcttcat ggtcagccgg
    12661 tatataccga ttaaaactgg attttccctg ttgacatcat actgattata ttcagcgtag
    12721 ttgtaatagc ccgccatctg aagaacacga tctgcgatga aatccttacg ggtacgatta
    12781 gattcaacga tagcctcaat caaatttcca ggaacatctg catagtttgc caacaactgc
    12841 ttggtatctt ttggcaagca ataacctcca tatccaaagg atggattgtt gtagtgagtt
    12901 ccaatacgag gatcgagaca aacaccattg attatctgct gagtattcag ccccttcatt
    12961 tcagcataag tgtctaattc attgaaatag ctgactcgca gtgctaaata ggtatttgca
    13021 aacagcttta ctgcttcggc ttcagtgaat cccataaaaa gagtatccac gttctcctta
    13081 attgcacttt tctgcagaag tgttgcaaaa acatgagcag catttaacaa gcgctcatca
    13141 gtcatatcag taccaacaat aatacgagat ggatacagat tatcatataa agccttgctc
    13201 tcgcgcagaa actctggact aaaaataatg ttcttactcc ccgttgtctc acggactgat
    13261 tcagtgtagc caacaggaat tgtagacttg atgaccatga tggtatcagg attcacgtcc
    13321 atcacaacct caataactga ctcaacagca gaagtatcga aatgctgagt acgactgtca
    13381 tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt tataagcgct ccttgcatcc
    13441 aaagtagcag ttaagtcaag ttctttttct gtaagatact tttcaatata ttcatcctga
    13501 attggagact tccgtttatt aatcagttca atcttttcag ggataatgtc taccgcggtt
    13561 acctggtgat gctgcgccaa tagtgtggcg atactcaaac caacataacc ggtgccggct
    13621 actgcgatgt tgtacttttt cttcacaata tctcctcttt cctctcttaa gtatggagaa
    13681 tgttactcct acggctattc atcatgtcaa aataacacag cttagaatgt tgataaaata
    13741 attttccaga atgtgataaa atctctttca tttataaacc acacatctaa ttttatctat
    13801 tctccacaag acatgtagag tattctttaa taacaagata cgaccttact aaatacttca
    13861 aaaaattatc atatttaatt cataaatata aatcttcatt ttaaatgtat gcccaaaaat
    13921 ttatctgtaa tgcgtcatca aaatctagat tccaaatctt tatcaatttt tatccactca
    13981 ttctaccact ttagtaatat tttctattta taacactttc gtcctacatt gatccccccc
    14041 tctgaagtac agtttttact gtcttaaata aaattttaaa atctaacccc atactccaat
    14101 tttttatata ttccgtgtca agttttgcta cttcttcaaa atcagtaata tcacttctac
    14161 cacttacctg ccacattcca gtaattcctg gcttgatagc aattcttgca cgatgatgat
    14221 aatcatattc ttcatactct gatttaaggc atggtcgaaa tcctataagg ctcatctttc
    14281 ctgttagaac gttgaaaaat tgagggaatt catctaaaga cgtggatcta ataaattgac
    14341 cgattcctgt cttttttgtt ccatctggaa gtattttatt tccgataatt cttggatcaa
    14401 actccatctt aaacataaga ttattgctca tcttattttc tttcattaat tctacttttc
    14461 tttcttctgc atccatatac atacttctaa acttatacat cttgaacggt ttaccattct
    14521 gacctatacg ttcttgtgca aagaaaattg ggcctggtga attaatataa ataagtggag
    14581 caataaagat aaaaataatt cccgttgcaa tacatccaac aatacctgca agaatatcaa
    14641 ttgttcgttt tataaatgcc tgttgagttg tcataacatt gatacttgtt gtaagtactg
    14701 tatattctcc tatacgacca atagattgtt ttcccaaagc actatctgat acttttgcaa
    14761 gattcaaatg tacagtaagg cccatttcag agcatcgttc aataaattct ttcatacacg
    14821 aagtactttt attcaaaata ataaagactt catctaccca tccctgacat aagtaatccg
    14881 ctgcattttt ggaatcagaa acaactgtca ctccctcaac atcttcacca atcatgcttt
    14941 tatctatgat aatgacacca tttattttat acattttata gttatgctct ttcacattac
    15001 gaataactac ttcagcaata ctactagatg taactataat aagtgatttg ttcccctcat
    15061 ttttcatttt atgtttcaag taatgtttcc ataagaggcg agttatatat gtaattacaa
    15121 cgtataaacc tcccatcaag aaaagaacga ctctagaata atcatttcca ccctgaacac
    15181 taatgagata caaacttcct aacagcacca acataatcgc ttgctttaca gaagaagcaa
    15241 attctatata gtaccctctt tttaatacac ctctatatgc ttcaaacatg aaaataacta
    15301 aaagatctgc cagttcaatg aagactgcca tgttttgata cattggaatc acataaggat
    15361 tccaatctac ctggcgtaag acatacgata gataaaaagc aagctggata cacaataaat
    15421 ctaaaataat aaaatcaata tgtttaaccc atccccttga acttttcttg tacattcctt
    15481 tttactcccc tctttttaat aaaataaatt gtattacttg ttttctctcc taaatatctt
    15541 tctgaaggat tattgactcg acaaattggc ttgtggatat tcttatatat agaacatccg
    15601 taagccaact taaaactcct taacaaaatt tttttactca tattgtagat caaaacaagt
    15661 atccaagaaa tcatatttta tttctttttt ctgcttactc aacaacagct aaaagaaaaa
    15721 tcatcataaa caaattcaac taagccaacg atccccttgt gaaatcaagt tttttcttga
    15781 tttacgaata aagagctaaa ataaaccaaa cttcttcttt tttacctctt catagactgg
    15841 ataactgatt tcatccccgt tgatcagatc ctttgcgact tgtttcatct gtactacttt
    15901 ttccttacca aagtcctttg caatctgctc atagcactct tttaagtaaa aggtgcgctt
    15961 agagacccca tgggcatcag aagcagccat ctgcacgaga ttatgtttga ccatcgtttt
    16021 ggctgttttc tggatgtctt tgccaaatac cccaacataa cttggcgcag tgagttgggc
    16081 caaacatccc atatctagga acggaagcaa atgatttggg tcttcgcgaa aatgcacgtt
    16141 gcgttctgga tggacgatta ctggtgtctt tcctagttct aacaactcaa aaaaaagccg
    16201 ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg tatcatctaa
    16261 atctgtaaat aatatttcat ctcggtggat gtcttcgatg agcgttccgg taatacgtac
    16321 ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc tctaattcgg cttgtaacga
    16381 agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat tgtggtgtgg
    16441 tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg caatacttgc
    16501 ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta gatcgatcat
    16561 ttctcttgtc tcctctcttc ttattcctta atttccgtag taataaccag catctttgct
    16621 atgttctgct ccgttgtaca caacacctaa gactcgtgct tgtaccatct ccagcagttc
    16681 ttttgcttta gtcaaggatt ctttacgtga catattttca cgtacgacca ataaagtgcc
    16741 atccgttttt aaagacatga tctgtgcatc tgttacggct actactggtg gcatatcgaa
    16801 tatcaccacg tcaaataact gtcttgcttc ggataggatc tgatccattc ttggcgagct
    16861 taacaattct gatgggttgg gtggcttagg tccactaggc aaaatcgaca agttctcgac
    16921 tggcgttcgt tggatcacat ctgctacact tcctgatgaa ctaagtgctg tacttaatcc
    16981 actggcattg ttcagctgga atgtcttata gacgacgggt ttacgtaaat ctgcatcgac
    17041 tagtagaact cgttgacctg atttagcaaa aacgactgca atgtttgctg cagtggtgga
    17101 tttcccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct gaccagctgc
    17161 ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg gtgaagatgg
    17221 gtttgttaat gtgatcaaac tgactgcatg agtttgtgct gctttttgtt tctgtgttcg
    17281 tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggggc tgttttatcg
    17341 tttgtagctt ttggtcgtgc aggagctgtt ccttttttac ggataccagc gttcaattct
    17401 ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc tgtcacatat
    17461 ttttcatctt tcactgtccg atcgagtaat tccaataaga atgccagacc tacacctacc
    17521 atcattccta acattaagcc agtcattaaa ttcaatttat tatttggcga aacaggtgtc
    17581 gtattggcaa ctgcacctga aatgatcgag attttatcaa cattcatcac atcttttgcg
    17641 ttctcttgga aaacttgtga agttgtgtta gcgatctgtt gagcagccac tgcattcgta
    17701 cttattgcct gaatcgagaa catttgtgaa ttttctgcct gattaactga gatggcatct
    17761 ttgatttgtt cagcagtcat tttcacattg tcttctgttt ctaaacgatc tttgacttca
    17821 ttgagcacta aatcactgac gatcatatct ttatacgtat tgatcatttg caggttcgta
    17881 ttgacatcat tgacatttgc agtgtctgat tgtggcagtg tcacgatcaa ttgtgcttgt
    17941 gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc caagccagca
    18001 aacattgaga tgatgattat taccaagtgt ttgcgtaatg tttcaaaaat ttctttcaag
    18061 ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt catttgatct
    18121 tttaattctt ttttcacacg atcaagttct tgatcactga ctttttgata ggagatatta
    18181 tcttgcatga agccttctcc ttgcagctga tcttgcttaa tcgtaccaaa tgcctcccga
    18241 taatctaaag caattttttt catttcatca aagttcatgt ctgttttcat attcgactct
    18301 agagcattca aaacggattg atagttgctg acaccgttta gactcaatac ttttttcgct
    18361 acgccttcta cgatctttct ttggcgccct tgtctaccgt aatcaccatt tgggtcttca
    18421 taacgcatac gagaataaga taatgcctga tccccgttca atgtaatttt accgattgga
    18481 aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc accaactgca
    18541 tcaacgagtt cctttagtcc tttcatattg atagagacat agtgatcgat tgggatgtct
    18601 acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta agcgtggttg
    18661 atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg aatgctgacg
    18721 atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc tgatcttcct
    18781 tgatcgaccc gacctaaatc tccagtatca atccccatca atagaacaga gaaagattct
    18841 tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac tgattcgtaa
    18901 gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc tacaattgct
    18961 aatactaata cgataagaac tgctaaaata atcaaaaata tttttttccc tcttgatttt
    19021 cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca acgttttaac
    19081 tgtcaatagc ttatattgct atagcccatt tgctgagcaa cagtttgtgc ttttgttgcg
    19141 tatgcggctc ttattttttc attccggatc tgctctattt gactgagcgc cgtcaaatat
    19201 tgatcgtaag tagcggtagc tgttacttga ccgtctttca gcatcgtatc gaatagctga
    19261 tctagttgcg tgacttgtgc aatttgggta tcagcgaatc ctagatattg cataacgact
    19321 gttttccaag cactatcctt aaaaaagctc atattttcac gtacatgggc gacatctgca
    19381 cttgccaact tttctttgat gatgacatca tccacagctt gctgccagtt tgatacgttt
    19441 ttagtaaata atttattgac agccgtctgg atcttcagtt ttgattctgc atcctctaac
    19501 tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag cttctttagg taattctgat
    19561 tccttgatgt cataatcatc tgccgaaagt ttgatagaag atagtttatg tgacaaattt
    19621 gaaactgttt cttctgttat ttcaggattt aggaaaattt tttcttttgt actgtacagc
    19681 ttatctactt cttttgataa agaggtcacg gtgtcttttt gattggtgat ggcagattct
    19741 gcgtaattga tccgttgatg atattgccgc tgattattga gatggtatcc aaacaagcaa
    19801 atcacaatag cgcctgtgcc taccgccaca gtagccaata acttgacgaa cggcttttcc
    19861 atagattcac tcctaaatat tgatgataga taagtaaaga tgtgattcat tcacatcaga
    19921 atgagcgctt tttattttaa aagcataatt aaactaatat attcatagaa atatttataa
    19981 acaggttttc cctatataat cataccacat acagtttttt tgactaggga aattcctaat
    20041 ttaaaagtaa ataaaacagt aacaagatta taattaatcg ttttcatttt tatttcctca
    20101 cttttcatga gcgttttgaa aattcgaaca aagaataaaa taacaaaaat tattaaaata
    20161 gaattaataa cagctattta taacaaaaaa aacagagaca cagcacgttt cgtgcatgct
    20221 ctctgttttt caaacaaaag actattttgt gatgatttga tccaagcttg ctaatgacaa
    20281 agccatggca gctatttgac ttagttgtgt tagtcggttc ttacgtactg ctggttcatc
    20341 agccatgacc atcgtttgat caaaataatt ttcgatcagt ggacgaagag cgacaagctg
    20401 gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct ttcattgctt ctactgcttt
    20461 gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg tctacttctg attgtttttc
    20521 aagctgcgct tttttcgcta agttcacgac acgtgtcaaa gcttccattg aaggtttgaa
    20581 gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct gcaaatactt ttgtcagatc
    20641 tttttgttcc gcattcaaaa cagcatcgat gacatcatga cgaatattct tcgttgtcaa
    20701 taattggcgc agacggcctt tgataaagtc gatcacttgt tgttgccctt ctgataaacc
    20761 aatgccgaaa cgatccgtat ctttactgat aacatcgcta atcgtttctt gcagaacaga
    20821 caaaggaaag gcccattttt tcgattcgat gatccgaacg acaccgtatg cttggcgacg
    20881 taaggcataa ggatcgtttg acccgcttgg aatcatccca actgcaaaga aagaaaagat
    20941 gctatctaat ttatctgcaa tcgctaaaac agaacctact gtagactgag gtagctctcc
    21001 ttcactggat ataggcatgt aatgctcacg aatcgctgtc gctacagcag gtttctcgcc
    21061 tttcagaaga gcatattttt cgcccatgat cccttgtagt tcaggaaatt cccctaccat
    21121 gttggttact agatcaaatt tatagatttc agcagctcgt tgcaaatcca tcaattcttc
    21181 ctcagatagt cctacttgtt cgccaatgat acgggcaatg acttgtaccc gttgcatttt
    21241 ctcatagata ctaccgattt tttcatggaa agtaacagtt tttagttttt caacgtattg
    21301 gtcgattgaa gctttcttgt cttcattata aaagaattcc gcatcttcta aacgagccgt
    21361 caatactttt tgattgcctc gaaccacatc ttccaatttt acccgatcac cattacgagc
    21421 agaaataaaa tgaggaagca gcataccttg atcattccgt acctcgaaat aacgttgatg
    21481 ttctttcata gaagtgacta gtacttcttc aggaacagaa agatattttt cttcgaagtc
    21541 gccagaaaag acagttggat attcgactaa attgttgact tcttctagta aatcttcgtc
    21601 tagatcaata acccagtgat ttttttctgc taattcgttt gcttgtgtaa cgatcatttc
    21661 ttgtcttttt tgcggagacg caatgacgaa ttgttcattc aatttttctt catattcttt
    21721 tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga cctcgagagg ctctgcctgt
    21781 tttgacatcc aagatttcga atgggattac ttcgtcatct aacaatgcca cgatccagtg
    21841 aatagggcgg atatattcaa aatcgtattc tgcccaatgc atcgttacag ggaaagtcaa
    21901 acttttgatc acccctgcta agcctgtcaa tacttctttt gcttccttac catgagtgta
    21961 tttcgttaca tagacatatt ccacaccttt taattctcta aaagtgatag cgtctgtcgt
    22021 cagaccttgt ccgcggacaa atccttccgc tgctttcgtc cagtttccat ctgcgtccaa
    22081 agcaattttt ttggctggtc ctttgacttc ttcttcactg tccgcttgtc gttctggaat
    22141 atttgttact ttgattgcta aacggcgcgg tgtggaaaat gtttcgatcg tatcataagt
    22201 caattggttt tcatctaaaa attttgctgt tttttcttct aattgtttca tgcttggtgt
    22261 gacaacatgg gcaggcattt cttccagccc gatttcaagt aataagtctt ttgccatctt
    22321 attcagcctc cttcacggtt tctggatttg ctgtgttttt attcaacaaa gggaatccta
    22381 atttttcgcg ttctgccaca aagattttgg ctacagcacg agccatgttg cggatgcgag
    22441 aaagataacc ggcacgctct gttacggata ctgcaccgcg ggcgtctaat aagttgaatg
    22501 tatggctaca tttcaagata taatcatacg ccggatgaac aagattttca tcgatacagc
    22561 gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag caagtcttgg tcgcttactt
    22621 caaaagaata tttggaatgt tcatattctg gttgtttgaa gatctcgcca tatttcacgc
    22681 catcagccca ttctaaatca tagacacttt caacttcttg gatataagaa gcaagacgtt
    22741 ctaatccata agtgatctca gaagttactg gtttacaagg cagtccacca acttgctgga
    22801 agtacgtgaa ttgtgtgatt tccattccgt caagccatac ttcccaaccc acaccggcac
    22861 agcccatgga tgggttctcc cagttgtctt caacaaaacg gatgtcgtgt tctaatggat
    22921 cgattcctaa aaggcgcaag ctttctaaat aaagctcttg aatattctca ggtgaaggtt
    22981 tcatcaccac ttggaattga tgatgctgat acaaacggtt tgggttttca ccataacgtc
    23041 catcagctgg tcggcgagat ggctctacgt aagcagcatt ccatggttct ggtccgattg
    23101 cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt ttctgtgtca taagcttgca
    23161 tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt taaaatcata tcttggacgg
    23221 tcaatttttt actcatctgt tttgcccctt tcaattttaa agcgttgctg tcaaataaga
    23281 atacaggaaa gaaaacgaca aaaaatccct atgtctgatt acagacatag ggacgaataa
    23341 ttcgcggttc caccctactt ccgaataaag attcggcagc ttcatttatc gtactcgcaa
    23401 gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc ccgaactcgc taaaccacgt
    23461 gttttttact gatcttggtc aatgtacgta ttcattttgc agttttcttg tttctataat
    23521 agatcctatc actgaacgat ctatttaatc tatggttaat aatagacgga actccctgtg
    23581 tttgtcaaat ggttttgatt ttttgttgta tgttttattt actattcttc tttttcaggt
    23641 gtctcttttt ctttttcaga catgtctttc tctgacgtct ttaatacgtg tgcccaagac
    23701 ttcatttgat tgatgaactt tttgcttttc aaatggatac cgacatattc atcatacagc
    23761 tggtcgatga gttcacggat tgcttgtttg gtttcttctt tcagatggat tccactaatt
    23821 ttatcataag aaatagctga aaacatccga acgaaataaa cagctcgagg atctgcatga
    23881 taacggtgat cgtccatcga ccaatgtctt tcgcatatca caccaccata tttggaagaa
    23941 tagtcaaatt ttccttttgt ttccccgcat acgacgcagt gtgtccagat tggtgcaatt
    24001 ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga tgatttctgc atcgtctcct
    24061 tggtccatcc gttgcagtgc ttgttgcgta aactggaaca aatgcgggtc gtactgatga
    24121 tcttcgattg ccgcatctac tagattcaat aaatatgtgc catagccgct caaaaagatg
    24181 tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac tgcttaaaaa tgacaaacct
    24241 tcacttcgaa aattcccgat ataaactgct tcagtaaaag ggagcaaagc cggagctaag
    24301 ggctgatttt gccgatggat ctctttgata taaaacatca ttttgccaga agattctgta
    24361 aagattttga ctaatttatc tttttctttg taattacggc tagataaaat caagcctttt
    24421 gattctgcaa gctggctcat attctccctc ctaaaaaata aatctggaaa agcagattct
    24481 gccgatccag atcatttatg ttgcgatctt ccgcagcttt tctcaacacg cggattttta
    24541 ttcagtttat ttctatacct aatttacaag aagctcagaa tatcagtatt cgtcttttcg
    24601 gtagccgaag tcttgcagat agactttttt gtctcgccag tctttttgaa ctttgaccca
    24661 aagttctaga aagactttgt ctcctaaaag attttcgata tcttttcttg ctttcgttcc
    24721 tacttgcttc agcatttttc cgcctttgcc gataatgatc cctttttggc tatctctttc
    24781 gacaataatc gtagcctgga tatggatttt atcattctcg tttcgcttca tcgaatcaac
    24841 aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa aacaagactt tctcacggat
    24901 caattcagaa acgatgaagt attcaggatg atcagtcact tgatcatctg gaaaatattg
    24961 tgggccctct ggcatttcac tgactaatac atccatcaaa cgttcgacgt tgtttccttc
    25021 tgtagcagag attggaatca cttgtgcaaa aggcatctgc gttgaataat cttcgatgat
    25081 tcccaacaac tcgtctggat ggattttgtc gattttattg ataatcagat aaacaggaga
    25141 atgcacgttt tttagacgtt caataataaa atcgtctccc cgaccgcgtt tttgatcagc
    25201 actaatcata aataagaccg cgtccacttc tttcaacgca ctgtaagctg cttctaccat
    25261 aaaatctcct aaacgatgct ttggtttatg gattcctggt gtatcaataa aaatcaactg
    25321 agcttctggt gtagtgtata ctccttggat tttatttcta gttgtctgtg ccttgtcact
    25381 catgatggca attttttgac ccacgattcg attcaataat gtggatttcc ctacatttgg
    25441 gcggccgacg attgcgacaa accctgattt atgttctggc atatatgttc tcctttttaa
    25501 ttaaacaaca aatgatatat tttagggaca aataaaatca acccaacgat gacggtaaaa
    25561 caagcggtca ctaaaactgc tccagctgcc atatctttga tttttttccc aatcggatga
    25621 aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa cagtattgat gatctctact
    25681 atccatacga gaaaaacagc taaaagcagc cataaccatt ctatccgatc aagttggaaa
    25741 atcacgccca taaacaaagc gaggattccg aacgcaacat gtttcctcat gttgcgttct
    25801 tcctcaaaaa cagttttgac tccttggacg gcaaattcca cggacatcag gaaatgtttg
    25861 tttttctcta cgtttttatc ttttaagtcc ataggcatct aagatctctt tctgtaaacc
    25921 aaacatttct ttctcatcct ctggtgtcat atggtcatac ccgttgatat gcaagaatcc
    25981 atgaaccgct aaaaagccaa gttcccgatc atacgaatgg ccgtaagctt ctgcttgctc
    26041 tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca cgaggcaatg gattctctcc
    26101 atcttcaaaa atcactggga tttcgtcttc gccctcttct tccaaggcaa aactgatgac
    26161 gtctgtcggt gcatcttttc cacggtaatc acgattgatt acttggattg ctgcattgtc
    26221 catgaaagta acagacattt ctgtttcttc tggcaactct aaatatgatg ctgcaaattg
    26281 cagcaagtcg tcgatttctt tcattttctc ttctgagact tggtttgttt catccatgaa
    26341 ggtaatatcc attatacatc gcttctttct attcttcttg ctattcttct tgttcttctt
    26401 ttatcttctc aacttccgat ttcattttag gatatttgat ccgagaatgg aaggtactgc
    26461 ttaaaccgct gatcaatgat ttttctactt tacggatttc ttttaacgtc aatccactgt
    26521 cgtccaattg tccatcggaa atacgctctt caataagatt atggacaaaa ctttcgattt
    26581 tttcgctcgt tggatggtcc attgcccgta ctgccgcttc acaactgtca gctatattga
    26641 cgattcctgc ttcacggctt tgaggttttg gtccaggata acggaattgc tcttccgtaa
    26701 cttccgggtt gcgttccttt gccttaacgt agaaataacg catcagcgtg gttccatgat
    26761 gctgtctaca aatatcgatc accatttgcg gcatattata atcttccaat attttggcac
    26821 catctgtcac atgtccaaag ataatatgtt tactgtcttc tggcaataag aaattgtgcg
    26881 gattttccgc tcctgaaggc aagttctcaa cgaaaaagct ggcgtgtttg atcttgccga
    26941 tatcatgata atagcaagct actcgagtta gtaaagaacg tccaccgatt tctgctacag
    27001 cattagcact tagattggct accatcatgc tatggtgata cgtacctggt gcttcttcca
    27061 gcaactgttt caataaagga tgattgggat tgctgagttc atttaatacg atcacactat
    27121 catcattgac catcaattca atataggggt gcagacctat cgtcagcaag aatgaaaaca
    27181 gtgcacctgt gaatccgcag actagcgtta accaagtcgt tgcatcccca aagctcattc
    27241 cttggtagat aatcaaaatg acatccatca tcacaggaaa aataatgacc cacatagcag
    27301 cggataaccc ttgttctgag atccgtttgc gtctgacgat cgttgccatg agaccagaaa
    27361 atacatagga catcaaaata actgtcagaa agttcgtgcc aatcgaatca taaaaaatga
    27421 aaagtgcgga tactgcttga aaaataccag ccatgatccc tgctcttcga ttcaagaaaa
    27481 agcttaaaac aagtggagca aaagccgcgg ggtaaaacag cggaatataa gcagcttgtt
    27541 ctgtttggaa gagttggaaa aatttcatta gtacgacgct tactgacatt gccgtcacgt
    27601 agaaaagtac gtattttgtt ctttctcctg cattttggaa ttgaagtgaa ttataaatca
    27661 gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat gagtgggaaa atagaagtcg
    27721 tttgattcgt caatcctagt aattccaatt ttttcatcgc tgaagcatca atttgatttc
    27781 cttcgcgaac aataatttct ccttggaaga tcatcactgg ttgtacagct tctcgtgcag
    27841 attgttttaa ttcttctgtc tttttctcat tcaatacatc attaactacg atgccttgat
    27901 ccactaagta gcgaatcatt tgttgctgct ctgttgtgac attcaaatat tgaatctgat
    27961 cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg aacttgttta ctcatctgtt
    28021 cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat ttccgtttca gtcatttcaa
    28081 atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa cgcaacgttt tctgcattca
    28141 cgttttcaaa atttttcttc agtgtagcaa tccgctcttc tgtagtaggt ttcgggatat
    28201 tttcatcttc cttcgcctta tctaccttgc cattataaga tttgttgatg gtatcattcg
    28261 tcttatcgat caattcgaat aattgcttga tccgattgtt ctggtcatct gccagatctt
    28321 tttgataagt atattctgga gtcactgctt ctgctgccag ttttcttctt tgttcggttt
    28381 cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc ttctgctact tggccttctc
    28441 tgaaatcaat ggccttttgt ttgacactac taaatgtaac gccgcagaca atgataaaaa
    28501 acaagaacag aaggaaaggc aatgctgcct ttccgagttt ttcttgtagt ttctgcagtg
    28561 ttttattcaa catgtgctta tcctcctaag caaagcttat tcttgatgaa gatccgcctt
    28621 ttcataagct ttgatgattt cggcaactac agggtgccga acaacgtcac ttgcttcaaa
    28681 attaacaaaa tcgatttttt tgatttgctt taatgttcgt tctgcatgaa caagaccact
    28741 tgtcgtccca cgaggcaagt ctatctgact tgtatcccca ttgacaatca tttttgattg
    28801 ataaccgaga cgggtcaaga acattttcat ctgagcgact gtcgtgtttt gtgcttcgtc
    28861 caagataacg aatgcatcat ccaatgtccg cccccgcatg taagctaaag gtgcgatttc
    28921 gatcacgcct cgttccatca atcggttcgt atgatccaat ccaaaaattt ggtataaagc
    28981 atcataaaca ggccgcagat aaggatccac tttttctttc agatccccgg gtagaaagcc
    29041 taggttttcc cctgcctcca cagcgggccg cgtcaaaatg attttttgca cttcgccttt
    29101 ttttagggca gcaattgcta atactacggc aagaaacgtt ttccctgttc cggctggccc
    29161 tacaccaaat acgatgtcat gtttggctac cgattcaacg tatttctttt gaccgctgtt
    29221 ttttacccgt ataggttttc cattacggtc tttgacgatc tcttcctcat acatctcaac
    29281 aaaataatct aatgtacctt tttgccccat tttcaatgcc gtcacaacat ctggcgtact
    29341 tacgtggatt cctcgtttga tcaattcctg taatgtccga atgactgagg cagccaagga
    29401 aatctgggat tgctcaccaa tgatctgaat gatttctcca cgtgtatgga tggttgtttc
    29461 cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca cctaaaagca tgctgacatc
    29521 atcagcagct gtcaatctaa tctctaaaga actagactct tccgtcaaaa atagacatcc
    29581 cctttttttc gattcattct ttcctatttt accatactca gaagaaacta tatagcaaat
    29641 aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca agaaacgtta aaaaatagcc
    29701 gtttccattc gctaaaggtc tcgttttgat aaaaagaagc tgagaatgta agttatatga
    29761 aagtggagaa caaaatattt cgtttcattc aacaagtatt ctctaggtat ttttcctgtt
    29821 tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt tcaaaaaaaa caggtgtatt
    29881 attacgtcaa caaataaaca tacagcaaag cgtcgaggaa aacagtaggc tattcaacga
    29941 tttattccca gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat
    30001 ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga
    30061 taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac
    30121 cgttctagaa tctagagccc ttttcccaag tggaaaaggg ctttttttta ttttaaggtg
    30181 gtggttttca tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc
    30241 aatcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga
    30301 aacaactatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctaa
    30361 tctttcctgt ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat
    30421 ttgctatctc aggtgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa
    30481 gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa
    30541 tccttgttca tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt
    30601 ttgaaatggc tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat
    30661 tctctgctct tttcttttta cttgcttatt tccttactgt ccatccaaca cggttggtca
    30721 aatacgtcgg aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg
    30781 tttctttatt ccatccaatg ggaaatctaa atcaatctgc tgcagatact tatcaaacaa
    30841 acgctttaac aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gctttgcttg
    30901 ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca
    30961 cagaattaac ggcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg
    31021 gacttgttct tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa
    31081 ctgcactttc tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag
    31141 tgatggtcac attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcgcaag
    31201 atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg
    31261 tctcttttgt cgttgcaaat gctggtttag acaatatcat ccaatggtct ttaccagtat
    31321 tgatgctttt gtatccattg tcgcttgcgt tgatcttgct ttccttaaca gcaaaatttt
    31381 tccaaaagac accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cctgccgtat
    31441 tggatatgct ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc
    31501 tcgaatttta tcatcatcac gttccttttg ctgcattagg acttggctgg atggtcccaa
    31561 cgttacttgg ttacgctggc agcctactgt tttattacgc ctatcgccta tcagggtata
    31621 aacaagaagc aaatgaacta ccagaagaat aaccaaatca aacacaattt aacataagaa
    31681 atattaaaca ggaaaaaacc agctagatga aagaatccta ttctttcgtc tagctatttt
    31741 ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat
    31801 gataaaatta agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt
    31861 ttcctttgac tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag
    31921 caccagtttg tgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt
    31981 ccggcatgta tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac
    32041 gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca
    32101 ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag
    32161 atgacttgat cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt
    32221 tcatgtcatc gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa
    32281 ctagaatttt ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt
    32341 ttcgtagaat tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt
    32401 gaagcgacga agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt
    32461 aattccctcc ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa
    32521 acaagactgt cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct
    32581 tttttttaaa attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt
    32641 ccgaaaatct cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa
    32701 ttcattggac acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg
    32761 tgatggccga tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg
    32821 gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa
    32881 cttactcctt tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga
    32941 ttgcgcttca ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta
    33001 tttttcaatg tttctagtga ttgttcaatc aatgtctgtt ttttcatgac ttattcctcc
    33061 aaatcaaaat gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat
    33121 gcaagtgagg ttttatccat gtcttccgat tctacagaaa tcattacttt ttttgtcatc
    33181 tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag ccactatggc ccaatatcct
    33241 accgttgagt tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag
    33301 gcactgcaag atgcactaaa agaaaaagcc cttgtcctac acacattgat caatcaagaa
    33361 ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg
    33421 actccgccga tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc
    33481 gcccttcatt tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta
    33541 aaatacgatg acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc
    33601 gtatccagaa cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaggtt
    33661 gccaatctac ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct
    33721 aaaaaaatcg tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg
    33781 atgcgctctt acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct
    33841 gaactcgatt ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct
    33901 tctttatcta tcgaagaaac agcttcactt attttgaatg ctttagacct agaagatcat
    33961 agttattatg gaactgaaac caccgaagaa acagactaac agtttttctt gtctttttct
    34021 taaaaaaact ggactgtgtc agcgaagaag attcgttgat acagtccagt ttgtctctat
    34081 ttgcacctat cgttattgat gttccacatg tttgagcgtc tggttcaaaa tatccagtac
    34141 gtgagaatca tcaagactat acaaaatcgc ttttccctca cgatgggact tgacgacatg
    34201 gttttctctt aataaacgca gttgatgcga tacagcagat tgttccattt ctactgcttg
    34261 ggtgattgct gtcacatttt gttccccgtc tttcagatac agcagaattt tgagacgagt
    34321 ggggtcactc aacattttga atagttggct gactttctct acttttgttt tttgcgtgtc
    34381 tggcaatcca ttcacctcca acttgaaaaa agactgactt ctaaagttta ccactttttc
    34441 catcagtctc aagttttttt attcttctgt catttttttg ataatcgctt cataagcggc
    34501 tactttttca gctgcgtctt ctgaagagtt tccttttaca ccgatataaa atttgatttt
    34561 cggctcagtt cctgaaggac ggatagcgat ccagccattt tcttccagcg tatacttcaa
    34621 tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa
    34681 ctctttgaag tcttctgttt ggatgacttt tgctccggca aattctgtcg gtgcttcttc
    34741 acggaatttt tccatcaaag cagagatttt tgcagagcct tcttgtccgc tcattgtcac
    34801 agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa atcgtttgca agccgtcata
    34861 caatgttttg ccttgttttt tatagtaagc tgctacttct gctaataaga gcaatgcttg
    34921 aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc
    34981 gaacataaaa gtatgggaat gatcttcttc aaattgttga attttttcag caatgaattt
    35041 gaatcctgtc aatacattga acatggtagc cccatagtgt tccgcaattg ctgtaggcaa
    35101 ttcgctagaa acgattgatt tcaataccgc tgcattttca ggtaaagtgc ctcgcgattg
    35161 gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt tggtttcctg tcaatacttc
    35221 ataggagcca tttggcaaac gaacggctgc gcctaaacgg tctgcatcag gatccgttgc
    35281 aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc gcatactcga aagcagagtg
    35341 ttcttctgga ttcggtgatt tgactgtagt gaaatccggg tctgcgacag cttgttctgg
    35401 aactagtacg aattgttcga atccagcttg tttcaacgca cgttcgccca acatttttcc
    35461 tgttccgtga agtggtgtgt acacaagctt caaatcttta cccattgttt ccaccaattc
    35521 atgatcaatc gttactgctt tgatttcttc tagataagcg gcatccactt cttctccgat
    35581 gatcgtgatc aatccgctat gttcagcttc gtcgtcagat aatacttcaa cttttaacgg
    35641 attgctgact tcacggacat atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc
    35701 cccatcttct ccgtagactt tatatccgtt gtaagctgct ggattgtggg atgcagttac
    35761 catgattccc gcaaacgtct tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg
    35821 taaactttcg aatacataag aagggatatt gtgatgtgct aatgttttgg ctgcttccat
    35881 ggcaaattct ggtgattgat ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt
    35941 ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata
    36001 gatgttcatt cgattgatcc caggtcctaa gattccccgc atgcctgcag taccgaattc
    36061 caacggtgca taaaaagcat cttcaagttt ttcaggatct tcacccagat catttaattg
    36121 tttttttaag ttttcttcta aattttcttc atgtacccat tgttcataca cttgttccca
    36181 agacatgaaa ataacacctc tttctgaaat atggttctta ataagtatag cattcactta
    36241 ttagatatga aagtatgagg accaaaaaac gcttaaatca agaaaagatt cttttatcaa
    36301 gcacggttac aagtattcgc ataaatcgga ttcagtagat ttcacgcaaa aaaaagagta
    36361 aaagaacaat tgaaaccgtt ctttcactct ttttaaactt atgcagttgt ttcttctaaa
    36421 gattccaagt atttatacac ttgttgtcca gcaatcccac catcaccaac agcagtagtg
    36481 atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg tatattcgtc
    36541 ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg tcaacccagc ttctctaaat
    36601 ggtgcagtta atggatccaa tcctacatag atgaatactc cgtctgctgc caaatcaaac
    36661 tcattgccag ttttgacatt acgtgctttc acacctgtca ctaccatttc atttccaacg
    36721 atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa tgcacggtct
    36781 tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac ttcagaagca
    36841 aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat cactactaga
    36901 cgtttgttac ggaaaaatgc accatcacaa actgcacagt aagaaacgcc acgtccagca
    36961 aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc gataatgact
    37021 gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc gtgatccttg
    37081 atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc ttcatacatc
    37141 ttataagcta gatcaggacc catgattgaa tcgaaaccag gatagttttc gatttctgcc
    37201 gtattgttca tttggccgcc aggagcgcct tgttcgatca tcaatacgga taagtttgaa
    37261 cgtgaagcat ataaagcagc agtcatccca gcaggccctg cgccaataac gattacatca
    37321 tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc aatgcgaaaa
    37381 aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa aaatccgctg
    37441 aaaaacaata gacttttata aatcattgat caatcgttcg aaagattttc tccatttgaa
    37501 tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa tgtgccattt
    37561 ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc acctgtacca
    37621 gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac tccatctaat
    37681 tctactgcat ctttcattgc ctggatatgt gctgccagat agtcgattcg ataatcatct
    37741 tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc tacaataaat
    37801 agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc taatggatca
    37861 atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc aaagatattt
    37921 cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa
    37981 aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg ttccattttg
    38041 atctgaatgc catttcgttc catttctttc aatgcataag cgggatattc tcctcgtgac
    38101 tggacatcaa tgaagaaata attttctcga tctgactttc tagaagccca aacatcttca
    38161 ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac tttgttctcc
    38221 ggatctactt catgagcgat cttcgttgcg atggcacttg caaccaactc atgatgtgcc
    38281 gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc tgcgcccatg
    38341 aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct
    38401 ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctggaa
    38461 cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca
    38521 agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaccca
    38581 gattcatttg gctctttttc atcgcccatt ggaaaaatac gactccaagc aatggacatc
    38641 cgataagtct tgaaccccat ttctgcaaat aaagcaatat cttccttata atgatgatac
    38701 atatcgatcg caatttttgc tggataaaaa tgattttcat caaattcaaa cattttcttt
    38761 tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac
    38821 ccacgtccgc cttcatcata accgccctcg cattgattgg ctgctgtagc tccgccccat
    38881 aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg cttgataatt
    38941 ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct
    39001 tgaattgcgg taaatactgc tgatgtgcaa taaacaactc atccatgatc cgttttgctg
    39061 tctttcctga tcgtataagc ggattgatcg taaacgcttg caaagctgtt ccataatcac
    39121 cagtcacggc tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc
    39181 tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg
    39241 cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag
    39301 ataccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat
    39361 ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat
    39421 ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgctgcgct cttgtaccaa
    39481 tcgtttgata ttcttctaaa gcatgttcca gcatttcttc ttcacgatag taatagcggt
    39541 ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatgtcaa
    39601 agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc
    39661 ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact
    39721 tgtagatcag ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc atcgccatta
    39781 ctggcacatt gcataatccg atcactttat cccatttgcc atatctgata actgcttctg
    39841 tcaccatccc gcttgggttc gcgaaattaa tcagccaagc atttggacat aaacgcttca
    39901 tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat atcccgccag
    39961 caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg tctttgattc
    40021 gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt
    40081 cttcccgatc cagagttgca tagattttga cttcatacgg agaagcatcc cacatacgct
    40141 gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct acgagccaga
    40201 tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac tcgggggtgt
    40261 aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc tcactccttt
    40321 actttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa ttcgtttctt
    40381 tccagtaaca tttacaaaag ttagtgtttt cttctggatt tttgtaaata tcttgtcaaa
    40441 aaatgtcttt attaagctat ataacataaa tccgtttaca aatcattttc aaaaaagtta
    40501 taatttacgt aaaaaaacaa gaagaaggta tctacatgtt attacgtgaa aaaatcaaag
    40561 aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc gattctccag
    40621 cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac ccgtccacac
    40681 tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt aatgcttttt
    40741 tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat cttccttttt
    40801 ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa cagatgacga
    40861 ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa caactaatgc
    40921 ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt tctcaagatt
    40981 tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact tttggtgaat
    41041 cgttctatga agcgtacaat tgcacaagtg atatgtgtgg gattctgatt tcttatacgg
    41101 gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat gtgccaattt
    41161 tagctttgac cagtatcggt gattcaacgc tatcttcaat gagtacagcc gctttgcgac
    41221 ttacgacacg cgaacgactg tattcgaaaa ttggtaattt tacgatcaat acatcgattt
    41281 gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa cagaatttag
    41341 tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca gccatcatga
    41401 aagagccttt caattttgaa aataaagaag tggactaaaa agaatagtta agcctagtcc
    41461 attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg gcccgaccgt
    41521 ttccccttga ttttccaaaa tatccaaata atccatgtaa ttgatgacaa tcacaggtgt
    41581 ttctaaactg tatccttcct cttcgatttt ctttttgtcg aagcgaagca acagatcgcc
    41641 ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt ccaactgaac
    41701 agtatccaat ccgacatgaa tcagcaattc cagacctgct tcagaaacca tcccgattgc
    41761 atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa ggggcacgta cttctccttt
    41821 ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg catcctttgc
    41881 tgcattcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt tacctgatct
    41941 tttctgtcta tcatctgttt catctgcttc ctccttccaa aagaaaaaag tcagcgcaaa
    42001 ggataccacc atcgcaatgg cgatcccaat caatacatta atcacatctg ttaaatcacc
    42061 atctggtcta atatagttgg gaattgtaaa gatgcctagt ccaccttgat tccaagaagt
    42121 taaattcatt aacccactat acgcgccccc taacgcacca ccgacacagg taaaaataaa
    42181 tattttttta cgtggcaatg taaacccata aatagctggc tctgtaatcc ccacaatacc
    42241 agaaacaatg gctggtattg caattgcttt gtcttttgga tttctaagct taaagtagag
    42301 tgcggtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca taatattttg
    42361 ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat ggagaccaaa
    42421 catcaccaga atttgccaga agaagcctac gattgctcca taaataactg gcgaaagtgc
    42481 taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaata ctggtccaac
    42541 gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag aatggaacga agaaattcgc
    42601 gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta acaacacaat
    42661 aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa tgccgaatac
    42721 tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtcgtta gtggtgcttc
    42781 tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg cgccaagcaa
    42841 catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga taataacagg
    42901 cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc cagaaccgcg
    42961 gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca cgaatcatcc cgtcagcagc
    43021 taggatagca aggaatggtt ggaaacagcc cgatattcca tcaataagca tatcaaacgg
    43081 tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt ttataacctc
    43141 ttcaaacacg actggcacat gattcccgat gacaacttga tactgccctc ccgctcgcat
    43201 taccgttaca ataccgtcca tatttttcaa gatctcatca tttgcttttt cttcgttttt
    43261 taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt ccttcccgcc
    43321 gacattttct acgatttttc tcgctaattc ttggtatttt cccatttcta ttccctccaa
    43381 tttattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcata cgttcttgtt
    43441 aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac gcgcagtatg
    43501 gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaacgtct cggctaaaaa
    43561 gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa taacctgaaa
    43621 caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc gtgtcagaaa
    43681 aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt
    43741 atagtaatag cggacgatat ttagaatatc ctgtattgtt atagtgaaca ggtaggtatt
    43801 gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa atccggcttc
    43861 atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt tttttcccga
    43921 tggcatactc tttaggaaat agttgtttga tatcatacag caatacattt ggtacaaata
    43981 ttcccatttt gtgacgttcg atagccgtat acaaatgatc cgtcaatgca atatacacag
    44041 aatcattcaa ctggacttgt agagcctctt ttgtatattc aatgatttca caagaaattt
    44101 ctaagtactc aagaggaatc gcttgtgcca attcttggaa ctgttgtgaa agcccgtcat
    44161 ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat cgggtcaccc actttctttt
    44221 tgaatgccaa accttttccc atcacgatca tttcctgatt tctttcatcc ctggttatta
    44281 cgacgttgtt attcagtact ttctcaatca gcatgttttg ctcctttcca tgatatatag
    44341 ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc catggaacca tgggagactc
    44401 atcaggttta gcttgcatca ggcaatcact atccaagttg tatcgttttc tttatattat
    44461 cgaaagcaaa cgcttttgtc aacgctttct tagtggttat tagccgcttt tgactagctg
    44521 tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc tcacagatga agaatctgcg
    44581 aagctctgtt ttattttcac tctagcgctt agggcaaaat gctactatgc tactgtcaca
    44641 acctcttttt acacaccact gctttgcagt ttgtacatat ctgcatacaa accattttgc
    44701 gctagaagtg tttcatgatt cccacgctct actatatgac ctttgtctaa aactaaaatc
    44761 aattctgcat cccgaatcgt ggacagccga tgggcaatcg ctatcgttgt ccgtccttgt
    44821 cgcatttttg ccaatccttc ttggatcaat ccttcggttt cagtatcgat atttgctgtt
    44881 gcttcatcca atactagtat tttaggatct gtcacgattg ttcgcgcaaa agtgatcagc
    44941 tgtcgttgtc cactagaata gctggcaccg tgttcgatca cttttgagtg atatcctttt
    45001 ggtaattcat cgatgaactt gtttgcttga acaaactcag ctgcatggcg aatctgttcg
    45061 tctgtgatag ctggattcat catccggata tttcctgcaa tatctccata aaacaagaag
    45121 gcatcttgca agaccaatcc cattttctta cgtaactctt ccattggata ttctcgaatg
    45181 tcatgatcat caatcaagat ttggccttca taaaattcgt aaaaccgcat caatacgttg
    45241 atgatcgagc tttttccgct tccggtgtgc cccactaatg caacggtctc acccggatta
    45301 gcgacaaaag agatattgtt caatacattg tgctctccat cataagaaaa gcttacattg
    45361 cgaaactcga tcttacctga agtaatcgct tgatctgctc caggattttg ttgcggtgca
    45421 tattcttctg tatccatgat tttcaaaata cgactaccgg caaccatccc atctgtaaag
    45481 gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa attcctggac ataggtcgta
    45541 aatgcataga tcagtccaac ttctacggga gaatgcaacg catcgatccc aaacaaagtc
    45601 aacgccaacg caatcgccaa tgcatagaga aaattgatga ttggacttaa cagaatcgaa
    45661 ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt tgttcacttg ttcaaattct
    45721 ttagctaaac gagcttcttg gcgaaactgc tggataatac gcatgccgga aatcgattca
    45781 ttcaatttcg tgttcagctg acttaatttt tcccgcatat ttcggtaaat acgagaactg
    45841 aatttctggt aataccagat gacgattcct aaaatcggca agaacaccaa agtccacaag
    45901 gcaatttctc cattgatttg aaacatggca acgaaagaag aaacgactgc aaagatccca
    45961 gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg tttctgtgtc attcgtcact
    46021 cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac gcatacctag tgtatgcagc
    46081 ttttcaaata atcttacccg aacatactga taggtcttca atgaagccat ggaatacaaa
    46141 taccattgga aaaaccaaat gatacttttg actatgactc caaacaagta cacaccagca
    46201 aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca gatgatcatc cataaaagtt
    46261 tggatgatcc ttggcaatag gacattgatc acagacaggg caaacgcaaa taagatagca
    46321 acgatgaacg ttcttctaaa aggtttggcg aacttcatca gtcgtttgat gatcttcttc
    46381 tgttctttaa gtggaatcga ttttgtccat tccgattgat aattttcatc catcaggcct
    46441 cgcctccttc tatcttcgct tctaattgct gtttttccca cattcgttca taccagccac
    46501 ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat tttcccttga tcgacaacaa
    46561 tgatttcttt tgcatgcatc acgctgctta aacggtgagc tgcaatgatg gttgttttct
    46621 cttttcttat ttcttttaaa ttatgcaaga tagcttcttc tgttttggca tctactgcag
    46681 aaagtgcatc atctaagatc agcaattcag gatttttgat caatgctcga gcaatcgaaa
    46741 tcctctgttt ctgtccgcca gataaagaga ctccacgttc tccaactaag gtatcataac
    46801 cttttggcaa ttccttgatt tcattgttga taaatgctaa agaagccgca tgctctactt
    46861 cttcttctga aagttttgga ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca
    46921 ggaagtgatc ctgaggcaca taaccgattg atcctaataa agcattcaat gaatattctt
    46981 tgatcgaacg tccgccaaat tggattttcc cttgataatc atcgtattca cgcatcagta
    47041 atttgatgat tgtcgttttt cccgcaccag ttttcccaac aatacctaat gtttctcctt
    47101 cttggatcgc aaatttgatt tgttctaatg ctggtgattc ttctttcgga tagttaaaag
    47161 aggaaatagc tacattcagc tcgccttttg ccggggtctg cactgcaccc agttgttcca
    47221 cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc ataagaagca tttcctcttt
    47281 ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca aacaagcata ccgatatagc
    47341 taataaatga gaccatctgc ccgattgaga tcgtgccttc caagacatac tttccaccta
    47401 aaataattgt tgcaacataa gacagaccga taatcaaggt aatgaatgga tcaaataagg
    47461 agtctaaaaa attgactcgt ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat
    47521 ccgcgagatc ttctttttct tgtccaaatg ttttaatgac tttgatccca gtgatacttt
    47581 cttgtgtctt atcattgata ctagaaaaag cttcttgcga atcacgaaaa gcatcgtgga
    47641 gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg cagaggaatc agcgcaatca
    47701 gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat cgttgttccg cccgtaatca
    47761 ctgaatctgc aaaggtcaag attccagctc ctgctacatt ctggattgca ttcaaatcat
    47821 tcgttgcatg agccatcaaa tctccagtcc gatatttttg gtaaaagaga ttgtccattt
    47881 ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc caaccgagct gcacttcccc
    47941 aaatattcgt ccgccagata taacgaaaaa gatattgtaa gaaagctgca aggatcaata
    48001 ataagatcca ccaggaaatg atctttattg ataaattctt atcggcgatc tcatcaatga
    48061 tgatcccaat cacttttgga ggaactaact gtaccaaggc cactgcgaat agggagaaaa
    48121 tcccaattaa ataatgtttc ttttcctgac gaaaaaacca acctaatttt ctaaaaatcg
    48181 acattttact cctcctttgt ctttatcaag agcataggat tgtctgtgct tgtctttttt
    48241 gctgaatagt ctgaatagtc taacaaatta cgaaataaaa aacggactga aaaaatcagc
    48301 ccgcaaaaag tctttgggtc agaacctatt ttttcccttg gttcttcatt tgctgcatca
    48361 tctggcgaat tttcttctca gatggttttt gccccattga catcatcatt gatcgcaaca
    48421 tgtcctcatt aacgggagga tttttttcta aatagtcttt catatattta cgcgctagga
    48481 aaaagccacc tactgcgccg attagcaaag caataatagc aataagtacg acaatacctg
    48541 ttgataccat ataaatctct cctttctatt tccgaattgc tctcaatgat ttattttact
    48601 aaagtttatt gaaaaaagaa aggcgtattc tttagtttca tgcaaaaaat agtggttttt
    48661 ttagagaaga acaaaatttc tttcattaac cagcaagaca caccacgaaa aaaaattagg
    48721 cataccgaat ctttttcttt aattttttct ttctgaggtg tagaatgtaa tcgtagataa
    48781 ataggaaagg tggttttttc ttgaataatc aatccaacaa acaatttcat aagcatcgac
    48841 ttattgaaca tacaaatggt ttatctttag ctgaagtaaa tggaacagtg gatgtaccaa
    48901 agggaaaaag tttttttaaa acacttcttg cttactctgg accaggtgca ttagtagcag
    48961 ttggttatat ggatccaggt aactggtcca cttcgatcac tggtggacaa aatttccaat
    49021 atttattgat gtcagttatt ttgatgtcta gtctaattgc tatgctttta cagtatatgg
    49081 cagcaaaatt aggaatcgtg acccaaatgg atttggccca agcaatccga gcacggacaa
    49141 gtaagacatt gggtgtcatt ttatggatct tgactgaatt agccattatg gcaacagaca
    49201 tcgcagaagt cattggtgca gcaatcgccc tctacttact ttttgatatt ccattggtga
    49261 ttgccgtctt tattactgta ttagatgttt tcgttctctt actactgaca aaagtcgggt
    49321 ttcgtaagat cgaagcactg gttgtctgct taattttcgt cattttattc gtttttgtct
    49381 atcaagttgg cttgtctaac ccagattggc acagtttatt tgctggcttt atccctaaca
    49441 gtcatacatt ctcagactca ccggtcatcg gcggcgaaac tccgctgact ggagctttag
    49501 ggattattgg tgcaacagtc atgccgcata acttatatct tcattcagca gtttcacaaa
    49561 ctcggaagat cgatcaccaa aacgaagaag atgtagcaca agctgtacgt ttttctactt
    49621 gggattctaa tatccagttg tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg
    49681 gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc cttttttggt ctgtatgaag
    49741 ctttatctga tccatcagct atgagcaatg gtattttgat tgctgtagca aaatctggta
    49801 tcttgtccac tttatttgct gtagcacttc ttgcttctgg ccagaactct actattactg
    49861 gtacgttgac cggtcaagtc atcatggaag gattcatcca tatgcgtatc ccgatttggc
    49921 taagaagact ggtcacacga ttgctttccg tgattcctgt attgatttgt gttttgttta
    49981 caagtacaaa aggaacgatc gaagaacatg ccgctttgaa taacttaatg aatgaatccc
    50041 aggtgttctt ggcttttgcg ttgccatttt cgatgattcc tttactgatg atgaccaaca
    50101 gccaagcgga gatgggtagc cgatttaaaa attcattcat cgtaaaactt cttggctggt
    50161 tctctgtgat tagtctaact tacttgaact tacgaggatt accaggacag atcgaaagtt
    50221 tcttcggcga gcaagccact tccgctcaac tagcattggc tgatcacatc gcttatgtaa
    50281 tcattgttct tgtccttctc ctgttggcat ggacgattgg tgatttatat aaaggaaaca
    50341 aaaaatatga acagcttttg caggaaacgg tagaagaaat cgaataaaaa ctgatttttc
    50401 cacacaaaga cccttgatag ttaggttaaa aatctagcta ccaagggtct ttttatttag
    50461 aagataaagc ttttttcaga tttaatttac tgaaaactag catgcttgtt actacgatga
    50521 attgcgttaa tggcacgcct gcaccagtgt agacacttaa aaaatagtcc tttttttctg
    50581 gcgcataaga acaaaaccat gagacaccga aaagaaaaac caattgtgtc agtattaatc
    50641 cggtgaccac aatcaaagag gaactataat aggcacgatc agcttttgca aatacggttc
    50701 ctagtatcat aagtaatatc cctgctattg aagcaatcag cacgggaata gccagaactt
    50761 ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt tgttaattgg aaattaggtg
    50821 ttctcggttc attactatcg atccaattct ccattagcgg aaggtttaat aattcttgag
    50881 cctgtttgac ttgttcttgc gttccgaaac tataatttcc tccttgatgg aaatctttat
    50941 atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa tacgattggc agatcactca
    51001 tcgtctttcc atctcctgat cccatcagat agaactcttt tttctttttg atggttccct
    51061 tgtgataggc ttcttctaat tcttggttga cttttttcca aaaatcattg gtttcttttg
    51121 cattgttttg gtaataacca gattcgctta ccacatctcg caatgcccaa aagatgatat
    51181 ctccgggtaa ttcttttact tttccacctt gagtccacgc gtgagtcgta tataattcgt
    51241 caatacgggt agaaacagta tggaatgtag gagatgcggc taatgcttta tttagtgcag
    51301 catgtgagac ccatatctca ctgttttctt tattcaagtt cgtttcatca tctattcgga
    51361 tcagttgttt catcaaacgt ccaaattctc cactggaacg gtcattgatg gttgcaatac
    51421 cgtatgttgt ctcattttta tattcgatcc actgatagac gatggaaaag ccaactatag
    51481 gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga gagaagaaag agtcgttttt
    51541 ttaacttctt tacttctttg aaagaaaagt cttgccctag caagatctgg acgaaactga
    51601 tacccaaagc tactacagta aaaggaagaa gccagataga atcttcgcga atgaaccaaa
    51661 agaaaggaaa agcgacagaa agccctaaaa accatggcag caattttctg ctagaaccgt
    51721 atttgcggta ataaatagct aataaacaag cgataatgat ctcaacagcc ggaatcacga
    51781 gggcattacg atatgttcga gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa
    51841 aataaaaata gatacttaaa agaagatact tgttcttcac aatcggttta aacgcgagtg
    51901 caaccaatat actggcaaaa atactgaaca tgccgaacaa aactgcataa gaaagatgca
    51961 gtttatgtgc aagtactaaa aataagctgt aggagattcc tttggaaagt gttgtagtcg
    52021 tatactcccc tagccattta ccatccaaaa tattttgagc atagtgaatc agcagctgat
    52081 catcataggt cgccgacata tctgttccat agattgcttt actatataaa tacaaccgaa
    52141 taattgtaaa taatacggct atgccaagaa taaagtatgt atgtttcttt tttagttggt
    52201 cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt tcaatcacgt tttgctactt
    52261 gtttgtgaaa cttttcgttt tttacttttc tatcagtata ccctgtttgt ctgaatttaa
    52321 aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa aagcactcct ttgtttctta
    52381 aacaaaaaag gacaaggcag actgcccaat ccttttttgt tgctgtcagg tagaaaactt
    52441 aaagtgtgac ttcttctgct aataactcag ggaacacttc gggatcgaat gcttgcgctt
    52501 tgaacatgga aatttctttt ccatatggtg cgtaagcttt ctttttatct ttaccgacat
    52561 atggggtttc taatattttc gaaatgttgt caaacattgg atcatggacg attttgttca
    52621 atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg ccgatcttta tgagaacctt
    52681 gtggattttt agaatcattg atatgcagta ctttaagccg atctaatccg atgaccttat
    52741 caaactcttc cattacacct gaaaaatctt cttttatatt atatccggca tcattcgtat
    52801 gacatgtatc aaaagtaacg gataattttt cattcaattt tactccatcg atgatttgcg
    52861 ctaattcctc aaatgttcga ccgatttccg tacctttccc tgccatcgtc tccaaagcga
    52921 tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc ctcaataatt ctagcaatcc
    52981 cagcttctgc ccctgctcct acatgagcgc ctgggtgaag ggtgatttga gtagcaccta
    53041 aagcttccgc acggacaatc tcttctctca gaaattgtac agcaaatgga aacatttctg
    53101 gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg aacgacgata tttgttagtc
    53161 cgttttctgc cataaatgct tttccggcat caatattcat ctcttcgata gcttttcttc
    53221 tagtattttg aggagcgcct gtatagatca taaaggtcga agatccgtaa ctggctgctt
    53281 cttctgccgc gcctaacagc attttctttc cgctcatgct gacatgtgag cctaataaca
    53341 tggatggatt cctccttgaa aggcaattgt ttcgaacaat tcctgtatat tgataagatg
    53401 atgtacaaag aaaatggttt gtttttcttt gccctgttct ccatagtaac tgaaactttc
    53461 cctaaagaaa agcttaagaa ctctttcata aaaaagatca tttcctcacg ttttatctca
    53521 ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt tttttgtcct attatttgct
    53581 ataattagcg aaataaaacc attagttgag gtgacccttt tgccacgcaa aaatactcga
    53641 aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc ctaagattat ttttagagtg
    53701 tttcaatcat tgactgtttt tattactgtt cttcttttgt tgttcgcagc tctaggtatc
    53761 ggaataggtg ctggctactt tgcctatctt gtcgaagaca cacaattgcc gactaaaaaa
    53821 gaactgcaga cagaactagg aaacattacg gaaacgtcaa agattgttta tgcagataat
    53881 acagagatat caaaaatcca gacagatctg atgcggacga ctatttcatc tgataagatt
    53941 tcgcctttat tgaaaaccgc gattatcagt acagaagatg aatacttcga taaacatcaa
    54001 ggttatgtcc ctaaagctgt tttacgagca ctagtttcag aagcgactgg catcggctcg
    54061 tctggaggat ccactttgac acagcaatta gtcaaacaac agatcctaac cgatgaaaca
    54121 acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac aagtagaaaa acacttttca
    54181 aaaaatgaga tcattactac gtatttgaat gtctcaccat ttggtcggaa taataagggg
    54241 caaaatattg ctggagtcca ggaagctgct agaggaatct ttggtgtaga tgcaaaggat
    54301 gtgactctgc cacaagctgc ttatatcgct ggtttgcctc aaagccctat cacgtatagt
    54361 ccatacacga atacaggcgc gctaaaagaa gatctttctg ccggtcttgc cagaaaagat
    54421 tttgttttat tcagtatgta ccgtgaagga caaattacga aagaacaata cgaagaagcg
    54481 aaagcctatg atcttacaaa agactttctc ccacaacaaa tcgcggaaca gactgacaga
    54541 gaattcttgt attataccgt gatgaacgaa gcaacaagga ttattgcaca acaattggca
    54601 gaaaaagata atgctgatat gtcagattca tcggtcagtg aagcttatta tcaaaaagca
    54661 cagcaaacga tccaaaacaa aggatatacg atccattcga cgatcgataa agatatctat
    54721 gctgcattac aaaccggcgt agaaaattat ggctacatgt tagatgatgg tgctggtagc
    54781 caagtggaga ctggaaatgt attgatggat aacagaaccg gacgtatcta tggcttcgtg
    54841 ggtggtcgga attacagcca gaatcaaaat aaccacgcct ttgatacaga acgtcaagct
    54901 ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa tcgatatggg acttgtcgga
    54961 tcagaatccc gtgtatcaga ttatgcaacg acttggcaag aaggaaaaaa tgctggtgag
    55021 aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc aaaccgtccg tgagtcactg
    55081 gaatggtcca acaatatccc agcttatcac ttatatcaag atgtgttgaa ttccggtgga
    55141 tcgaaacaat acgcttacga gcactacttg gcgaagatga actacccagc aaatgataat
    55201 tggggcgttg aatcggctcc tttagggaca gtagatgtaa cgacgcttca acagacaaat
    55261 ggattccagg ccttagcaaa tggtggagtc tacgaagaag gctatatcat tgattccatc
    55321 acagataatg caggtaatgt gatttataaa catgaaagta attctgtccg tatttattcc
    55381 gaagcaacgg catcgatcat gaatgacatg atgcgtagtg tgatcaatga aaaaatcact
    55441 acaccattca aagatgtcat ctcaagctta aacggaaatc taggaaatgc agattgggtc
    55501 ggaaaaactg gttctaccaa tgaataccga gattcatggc tgatcgtctc aactccatct
    55561 atcactatca gtagttgggc aggacacgat gacaatactg gaatggacag ccaagcaaga
    55621 atacgttcgg ctaattatct tgccaatcta atcaatcaag cttaccaagc aaaccctgag
    55681 attttcggca ctgatgagaa atttgactta tcatccgatg tgatgaagaa aaaagtatct
    55741 gggttcacag gacaaacagc tggaaaagtt actgtcaata aaaaatccat ccaaacgcca
    55801 ggaaaaacag tggagtcact ttgggcaaaa aatggtccga agaaaagcgc ctttaaattc
    55861 ggcgtaggcg gtactgatga aaactataca gattactgga acaccgcttc agcttatgct
    55921 agagcgaatc cagagaccaa agaatcagac gataaagatg attgatctaa tagattaatc
    55981 agaattaaag ggagctgcga caaaaatctt gtcacagctc cctttttccg aataaatggt
    56041 gttcaaaagc ttacatctac gatattaaga caaatttatg taaaaatgtg aaaaac
//
LOCUS       DAFFQY010000005        55132 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFFQY010000005 DAFFQY010000000
VERSION     DAFFQY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.005"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            336..1439
                     /locus_tag="LRB02_000247"
     CDS             336..1439
                     /locus_tag="LRB02_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL4658487.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="LRB02_000248"
     CDS             1723..2403
                     /locus_tag="LRB02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL4658488.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="LRB02_000249"
     CDS             2493..3527
                     /locus_tag="LRB02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL4658489.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="LRB02_000250"
     CDS             3541..3876
                     /locus_tag="LRB02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658490.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="LRB02_000251"
     CDS             3860..6340
                     /locus_tag="LRB02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658491.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="LRB02_000252"
     CDS             6396..7955
                     /locus_tag="LRB02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658492.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="LRB02_000253"
     CDS             7952..9316
                     /locus_tag="LRB02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4658493.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="LRB02_000254"
     CDS             9487..10557
                     /locus_tag="LRB02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658494.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="LRB02_000255"
     CDS             10572..11240
                     /locus_tag="LRB02_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658495.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="LRB02_000256"
     CDS             11260..11838
                     /locus_tag="LRB02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4658496.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13153
                     /locus_tag="LRB02_000257"
                     /pseudo
     CDS             12241..13153
                     /locus_tag="LRB02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(13409..14326)
                     /locus_tag="LRB02_000258"
     CDS             complement(13409..14326)
                     /locus_tag="LRB02_000258"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL4658497.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14528..14965
                     /gene="zapA"
                     /locus_tag="LRB02_000259"
     CDS             14528..14965
                     /gene="zapA"
                     /locus_tag="LRB02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL4658498.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15023..15571
                     /locus_tag="LRB02_000260"
     CDS             15023..15571
                     /locus_tag="LRB02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL4658499.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15686..18046
                     /locus_tag="LRB02_000261"
     CDS             15686..18046
                     /locus_tag="LRB02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL4658500.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18133..18447
                     /gene="trxA"
                     /locus_tag="LRB02_000262"
     CDS             18133..18447
                     /gene="trxA"
                     /locus_tag="LRB02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL4658501.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18629..20407
                     /gene="uvrC"
                     /locus_tag="LRB02_000263"
     CDS             18629..20407
                     /gene="uvrC"
                     /locus_tag="LRB02_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL4658502.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20552..21451
                     /locus_tag="LRB02_000264"
     CDS             20552..21451
                     /locus_tag="LRB02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658503.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21444..22706
                     /locus_tag="LRB02_000265"
     CDS             21444..22706
                     /locus_tag="LRB02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658504.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22869..24767
                     /locus_tag="LRB02_000266"
     CDS             22869..24767
                     /locus_tag="LRB02_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL4658505.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25066..25785)
                     /locus_tag="LRB02_000267"
     CDS             complement(25066..25785)
                     /locus_tag="LRB02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL4658506.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26122..26454
                     /locus_tag="LRB02_000268"
     CDS             26122..26454
                     /locus_tag="LRB02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658507.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26441..28444
                     /locus_tag="LRB02_000269"
     CDS             26441..28444
                     /locus_tag="LRB02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL4658508.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28463..28933
                     /locus_tag="LRB02_000270"
     CDS             28463..28933
                     /locus_tag="LRB02_000270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL4658509.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28954..29538
                     /locus_tag="LRB02_000271"
     CDS             28954..29538
                     /locus_tag="LRB02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL4658510.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29553..30539
                     /locus_tag="LRB02_000272"
     CDS             29553..30539
                     /locus_tag="LRB02_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL4658511.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30529..30840
                     /locus_tag="LRB02_000273"
     CDS             30529..30840
                     /locus_tag="LRB02_000273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL4658512.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30862..32643
                     /locus_tag="LRB02_000274"
     CDS             30862..32643
                     /locus_tag="LRB02_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL4658513.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32636..34012
                     /locus_tag="LRB02_000275"
     CDS             32636..34012
                     /locus_tag="LRB02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL4658514.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34019..34654
                     /locus_tag="LRB02_000276"
     CDS             34019..34654
                     /locus_tag="LRB02_000276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL4658515.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34684..36039
                     /locus_tag="LRB02_000277"
     CDS             34684..36039
                     /locus_tag="LRB02_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL4658516.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36231..37625)
                     /locus_tag="LRB02_000278"
     CDS             complement(36231..37625)
                     /locus_tag="LRB02_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL4658517.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIYNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37717..38688
                     /locus_tag="LRB02_000279"
     CDS             37717..38688
                     /locus_tag="LRB02_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL4658518.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38685..39467
                     /locus_tag="LRB02_000280"
     CDS             38685..39467
                     /locus_tag="LRB02_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL4658519.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39636..40007)
                     /locus_tag="LRB02_000281"
     CDS             complement(39636..40007)
                     /locus_tag="LRB02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4658520.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40141..40584
                     /locus_tag="LRB02_000282"
     CDS             40141..40584
                     /locus_tag="LRB02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL4658521.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40627..41436)
                     /gene="yidA"
                     /locus_tag="LRB02_000283"
     CDS             complement(40627..41436)
                     /gene="yidA"
                     /locus_tag="LRB02_000283"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL4658522.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41455..42822)
                     /locus_tag="LRB02_000284"
     CDS             complement(41455..42822)
                     /locus_tag="LRB02_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4658523.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42806..43636)
                     /locus_tag="LRB02_000285"
     CDS             complement(42806..43636)
                     /locus_tag="LRB02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL4658524.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43799..44236
                     /locus_tag="LRB02_000286"
     CDS             43799..44236
                     /locus_tag="LRB02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL4658525.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44312..44932
                     /locus_tag="LRB02_000287"
     CDS             44312..44932
                     /locus_tag="LRB02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL4658526.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45121..45627
                     /locus_tag="LRB02_000288"
     CDS             45121..45627
                     /locus_tag="LRB02_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL4658527.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45644..46543
                     /locus_tag="LRB02_000289"
     CDS             45644..46543
                     /locus_tag="LRB02_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL4658528.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46630..47514
                     /locus_tag="LRB02_000290"
     CDS             46630..47514
                     /locus_tag="LRB02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL4658529.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGREIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47706..47777)
                     /locus_tag="LRB02_000291"
     tRNA            complement(47706..47777)
                     /locus_tag="LRB02_000291"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47742..47744),aa:Arg,seq:ccg)
     gene            47837..49171
                     /gene="rpoN"
                     /locus_tag="LRB02_000292"
     CDS             47837..49171
                     /gene="rpoN"
                     /locus_tag="LRB02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL4658530.1"
                     /translation="MKFEQNFSQKQQQTQKLTMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49320..50411
                     /locus_tag="LRB02_000293"
     CDS             49320..50411
                     /locus_tag="LRB02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL4658531.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            50452..51453
                     /gene="gap"
                     /locus_tag="LRB02_000294"
     CDS             50452..51453
                     /gene="gap"
                     /locus_tag="LRB02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL4658532.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51563..52753
                     /locus_tag="LRB02_000295"
     CDS             51563..52753
                     /locus_tag="LRB02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL4658533.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52857..53612
                     /locus_tag="LRB02_000296"
     CDS             52857..53612
                     /locus_tag="LRB02_000296"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL4658534.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53728..55026
                     /gene="eno"
                     /locus_tag="LRB02_000297"
     CDS             53728..55026
                     /gene="eno"
                     /locus_tag="LRB02_000297"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL4658535.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18290 a         9594 c        11670 g        15578 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaagg cgctagagga tgctggagca acggtttatt tgacccatac
    12781 tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa aaaaggcaga
    12841 tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa ctggaatcac
    12901 gacatattat tactatggac aagaggaaac attagcacag acaattgctg actccttcac
    12961 tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc ttcgagaaaa
    13021 tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg atttaaagga
    13081 attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag cattgaccac
    13141 ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat ttttcgtttg
    13201 gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc
    13261 ctgccgttta actccgcgtc acaaggagca atttcaaaaa acagcttctc atgtttgatg
    13321 actattgtga cttgtgaccg aggagttggc atgagaacac cgtttattcg gaacaaggga
    13381 ctgtgacatg acttttgtca cagttccatt atttttttag cagcttcaaa gctttttctg
    13441 tattggcaaa atgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata
    13501 gttttgccgc tacttggtca gatttcgttc cagctcctga tggaagggtg attccagctt
    13561 cagctgatgc tttgtgtaac tcttccaaaa agctggcacg actgatgata gaagcagcag
    13621 caacagctaa atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat
    13681 ttttttctaa acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa
    13741 tcgcttctgg tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca
    13801 aagcgacttt catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag
    13861 ataagacaag cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt
    13921 ggctatcttt taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat
    13981 cacgtttcac ataagcagca catactgtta gcggaccaaa atagctgcca tttccgacct
    14041 catcactacc taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg
    14101 ctggcttggc ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg
    14161 taccttgaaa catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg
    14221 cagcaaagct agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca
    14281 tttgactaag ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt
    14341 ttttatacat aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac
    14401 atagcatttt tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga
    14461 acggtgaaag aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat
    14521 gattcgtatg gcgcacgaaa aaacacgcta caaagcagtg attgccaacc aaacatatac
    14581 tattatcgga cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca
    14641 gttagcagaa ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat
    14701 ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa agaatcttgg agctggaaga
    14761 agaaacagca gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa
    14821 acgcattgaa gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga
    14881 tcataccatt aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca
    14941 aattaaacaa aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag
    15001 ttagaagaga aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc
    15061 gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt
    15121 attgtcattt attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata
    15181 cattccatac ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc
    15241 ttttcaccta gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg
    15301 ctggctgctt acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt
    15361 gagacaggta gactggattg caggaggcat cctgtcacta atcatggctt acgttactat
    15421 atttttgatt ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg
    15481 cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta
    15541 tgagctttgg gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt
    15601 tttgtacaga gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt
    15661 tcatagtgaa agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga
    15721 aaaagtcaaa cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc
    15781 agaattggta ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga
    15841 tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata cctaaacttg aaaatatccg
    15901 tccccatatg aaacggatcg agatcggagc agacttgaat ggagtggagc ttgcacaagt
    15961 ttccagagta ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag
    16021 tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt
    16081 gagccgccgt ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc
    16141 tgagttaaag agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt
    16201 agatgggatc gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg
    16261 aaatgaccgc tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt
    16321 ccacgatcaa agtgcttcag gacagacttt atttattgaa ccaaaacaag tggtggattt
    16381 gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag
    16441 tgaactttct gctgaacttg ttccccatcg tcaagaaatc atccacaatg cttatgtgat
    16501 tggtaaaatg gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt
    16561 gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga
    16621 tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat
    16681 tacaggacct aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact
    16741 gatggggcaa gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga
    16801 ggaagtattt gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc
    16861 ttctcatatg acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt
    16921 atttgacgag ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat
    16981 cttagatgat cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt
    17041 gaaagtttat ggatacaatc gggcaaatac gattaatgcc agcatggagt tcgatgtgga
    17101 tacactaagt ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga
    17161 aatctctagc cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa
    17221 tgatgagagc caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc
    17281 agagaccgaa tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga
    17341 tttgaaagaa gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa
    17401 gaaaaaagca aacgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga
    17461 tattcgtaaa atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga
    17521 tgcaaagacc caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt
    17581 gaagaaggcg aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta
    17641 cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat
    17701 attaaaaatg aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac
    17761 acaacgagtt atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga
    17821 ccttcgagga aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc
    17881 cattctagct ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg
    17941 caagggaatt actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc
    18001 ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt
    18061 aagcagatgg ttcgctatcc aagtatagaa atgctatact aatcatagat tttaaaaatg
    18121 gaggtctttg atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa
    18181 ggacttgtat tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg
    18241 attttggatc agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat
    18301 gtggatgaaa atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg
    18361 ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta
    18421 cgtcaaatga tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac
    18481 tgaatccagt tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt
    18541 ttttgagtat aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac
    18601 tttaattgaa tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt
    18661 acttcctgat cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt
    18721 cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat tttactggca gccataatac
    18781 gaaaacggaa cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa
    18841 tatcgaagca ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat
    18901 tatgttaaag gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg
    18961 tttggtgatt actcgtaaag tactaaaaga caaagcactg tattttggac catatcccga
    19021 tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg
    19081 caaacctagt cagacgaagg agccttgtct ttactatcat ttgggacaat gtctgtgtcc
    19141 ttattatttc gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaagtt
    19201 tttaaacgga ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc
    19261 tgaaaacatg gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac
    19321 aatcatgact cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta
    19381 tgcgattgac aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat
    19441 tgaacgagat gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta
    19501 cctcggtcag ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga
    19561 tacgatcgac aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg
    19621 aggagaaaag aaaaaattag ttgaattggc gaataagaat gcctcagttg ctttgaatga
    19681 acggtttgat ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg
    19741 aaacgcgatg aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat
    19801 ggggacgaat cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga
    19861 atatcggaaa tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga
    19921 agtcatctat cgcagatatt cacgagtgat tcgcgaaggg ttgcctttac ctgatttgat
    19981 cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt
    20041 aggaatcgat attcctattg ctggcatggc aaaaaacgat aaacataaaa caaatgagtt
    20101 gctattcggt atagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt
    20161 actccagcga atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg
    20221 cagtaaaaac agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa
    20281 aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga
    20341 attacaagaa atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca
    20401 ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca
    20461 aaaagagcgt ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat
    20521 atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac
    20581 ttttgggaac tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat
    20641 ggggctaatt ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt
    20701 tttgacacaa gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta
    20761 tgatgtcatc ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga
    20821 ccaactgctt tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat
    20881 cgatttttgg atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt
    20941 gtctaaagga aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt
    21001 gatcattttg gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga
    21061 tggaatattg gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga
    21121 taatgtagaa aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa
    21181 tggaaaagta catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg
    21241 tttaagccaa caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga
    21301 caatacgtta gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc
    21361 gactcagttc ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt
    21421 caaattgaaa gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt
    21481 taaaaaatgt taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg
    21541 gtatcattta tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg
    21601 tattggctga taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg
    21661 agttcaaagc agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg
    21721 atgcgtattt agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa
    21781 attcattagg acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga
    21841 tgcttcgagc aagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag
    21901 caagtttgac tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gttggagaag
    21961 ataactcgac agtccaatat gtcgtaagtt atgtagcaac gattgtcttg ttcattatta
    22021 ttttgacgta tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca
    22081 tggaagtgat cctgtccagt acgactgcac aaaagcattt ttacggaaaa ctaacaggtg
    22141 ttttacttgt tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca
    22201 gtcaattcaa agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact
    22261 tgattggacc attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt
    22321 ctgttttagc agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa
    22381 ttttgccagt gacttatcta tctctaggcg gatatatgct tggattgata ctaggagctt
    22441 cagatccaaa caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata
    22501 ttatgccggt tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt
    22561 tggtacttgt agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga
    22621 atgtactcgt gtatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta
    22681 tgagaaatga acgaaaaaaa gcataaaata tagaaaaata tagaaggaag ctggacattt
    22741 attgttccca gcttcttttt tgtatagacc tatttctatt tttttattat attggatata
    22801 tatattcatt gaaaatagag cataacaact ttataatgtt gagtatacca atttttggag
    22861 gcgttttaat ggaccgctat ttagatttac tttcagagaa gttccctaca acggaagatg
    22921 tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga acagagttat
    22981 ttttgagcga tatacatggc gaatttcctg cttttgatca tattctgcgt atcggttcag
    23041 gaaatcttaa agaaaagatc aaagaccttt tctctgaaca aatgacggaa acggagatca
    23101 gccaattcac tatttttacg gcttatccag aatatgtgtt gacgactgat tggtacaaaa
    23161 aacaagataa atcccagctg atccatcgtt tgatcgatct tttacggttt acgacagtca
    23221 aatacactcg atcaaaagtt cgaaaaggat tgccgaaaga atatagctat atcattgagg
    23281 aactcttgta tatcgatgat cgtatcaacg ggaaaaaaga ctatgtaaaa aaaatcattg
    23341 aacaacttgt cttgatgaag gaagaagaaa aatttttaac aaaacttgcg cagacgatcc
    23401 aaaaatctgt catcgatcat ttgcatatcg tgggtgatat ttttgatcga ggaactcaag
    23461 cagccaaagt aatggatcga atcatggatt tttcctctgt agatatccag tgggggaatc
    23521 atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttattg acgcttttac
    23581 ggattgccgc acgctacaac tatttatttg aattagagaa agaatatgga ttaaatttac
    23641 gtccattatt ttcttttgcc catcaaacct atcagaagaa tccagtattt acccctaaaa
    23701 acaggaaaga tctatctgaa agagagcaag aagaattaga aaagatccat caagcacttt
    23761 ctatcctcca attcaaatca gaacatcagt tgttgaatcg acgacctgaa tttaaaatgg
    23821 acgatcgtaa attgttggaa aaaatcaact atgaggaatc aacgatcgtt ttagaaggtc
    23881 agctctatga gttgaaaggg acttgttttc aaacgatcca gccagatgat cctaaaaaat
    23941 tcttggaaga ggaacaaaaa gtgatcgata gtttgatgta ttcattccag cactcgttaa
    24001 gaatgcaaaa acacatgacg tttttgatcg aaaaaggggg aatgtatctc acgaacaatc
    24061 agcatgtact gttccatggc tgtatacctg tagatgaaaa ggggcagctc ttagcatttg
    24121 aattagacca atcttatcgc ggaaaagaac tgctgggatt ttttgaaaaa cagataactg
    24181 aaagcgcaaa agatttgact gcaaatgaag atttggcgac cgatttgatt tggtatgcgt
    24241 ggagcgggcc attttcgcct ttattcggca agagtaaaat ggcaactttt gagcgctatt
    24301 ttctcacaga gcctcatacg cacgtcgaaa aaagcaatcc ctattatcac ttgcgagatg
    24361 aagagtggtt cagtgaaaaa attttggcag aattcgatgc aaaagaagaa ggttcagcaa
    24421 tcatcaatgg acacacgcca gtaaaagtaa aaaaaggaga gtcaccgatc aaagcaaacg
    24481 gaaaagcatt tgttattgac ggaggtctat ccaaagctta tcaaaaaaca accgggattg
    24541 ctggctactc attactatgc aattcttacg gtttccaaat cgtgacacat tatccgttct
    24601 tgcctattga acaacaattt gcaaaaaaat ctgatcaaac gaatgtaaaa aaagtgatcg
    24661 agcagcagct cccacgcaaa cttaatcgga atacgacaaa aggaatggaa ttacagcaac
    24721 agattactca actccaaagg ctgttggatt atcgaaagga cgattgagaa aaatgctagc
    24781 caacattgct caatagacta aaaagcgact gcaacaaaag gcgtgtcgca gtctccttcg
    24841 gaattagctc aaaaaacggt aaaatattag aagctatttt tctgtttatt tctctaatac
    24901 tcaggtcagg gcgctgattt cacgacctct tccttaaacg gtgttctcct gccaactcct
    24961 cggtcacaag tcacaatatc caacaaacat gagaagctgt tttttgaaat tgctccttgt
    25021 gactcagagt tgaacggcag gctcacgacc taatcagaaa aacgattaaa attcattcac
    25081 aaagaaatcg aataaacgct gttcagatga atctacacga tgagaaagtt ccggatgcca
    25141 ttggacacca aggattttgt gcatattatc tttactttgg actgcttcaa tgagtccatc
    25201 gttggaaaag gcgatggctt ttaacaaagg agaaagatct ttgactgcct gatgatgata
    25261 ggaattcaca aagtatgaat cagggagaag gtcacttaat aaactgtccg aaacgatttt
    25321 gacttcgtga gttgcaaact gcggggcagt gggctgttgt tcgtgtttta cggaccattt
    25381 tggatataat gaaagatctt gataaagtgt tccctccaac acgacattca atagttgaag
    25441 tcctcggcag actgcaaaaa tcggttttct ttgtttcaat gcttccaaga tcaatgcctg
    25501 ttcgaattgg tctcgttgaa tatttgtttc cgtcagtttt ggatgagggt cttccatgta
    25561 aagttgcgga ctaacatcct gaccgcctgc aagaatgagt ttgtcgattt tctcaattag
    25621 ttgaggcgct gaatctggag acatgactgg cagaataaga ggtaatcctt gagcatctaa
    25681 aactgcatcc acaaatccct gcggtgtata gcttacttga tttccttgga aagtatctgt
    25741 cgctcgaatc agttgattcc cggcaatgcc gatgattggt ttcattttta ttccccctaa
    25801 agtagactaa ttgtaacaag gatttaaaaa aatttaaact atgtaagact tgcccctata
    25861 cttatctgtt cttgctgtgt tactatctgt aaagagaatt ttcgtgtgaa attcatgacg
    25921 atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg
    25981 gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc
    26041 tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc
    26101 tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa
    26161 gcagaagaag aaaatgagaa gcgtgctcga accctacggc agcagttgaa gacctatgca
    26221 gaagaaaaag agcaagaatt ggagataatc caaacgaaca gtcggcaaca gctgaaacag
    26281 ttggtagaag aaaaagaaca attcgaaaag caagaattag aaaaagaaaa aatgaaattg
    26341 aaagcagaag cagacaaggt gcaagcaact ttgaaagacc aatatcaaaa gcatcagagt
    26401 caagcaatcg gtgcaatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga
    26461 aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg
    26521 aatccaaaat gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtaga
    26581 aaaatatttt ccagatacca cgattattga taaagaagca aaattaagcg ctttaggcaa
    26641 tcggctgaaa gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca
    26701 aaagaaactc catttgaaac atcaagaact ttccttacaa gacttggaag caaattattc
    26761 cgaagaaaat ttcatgaata aattagaaga agtccttgat ttaaaagaaa agtgggaaca
    26821 gctaactgaa aaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca
    26881 gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt
    26941 gagtgcagca aattttgaaa gtttcaagga agaactaagt aagcttgatg acgtttatat
    27001 ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc
    27061 agttgctgct atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta
    27121 tgaacaaaat cctaaagaaa aattgcaaga aaccaaacag caattatcca ttgttcacga
    27181 gcagcaaaaa aagataacga cagcgttagg tcagtgcagc ggctatatcc aagaatttga
    27241 atgggtagaa gaagtcacac ttgccgttgc agaacgggaa aaagtaaaag aacggtttat
    27301 ccatgccact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt
    27361 gatgcatgtg ttaaacgaaa ctgtatcaga agaagatata tacgttagtt tcgaaacacc
    27421 aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttatcga
    27481 accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac
    27541 gccttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta
    27601 cggattgctg atgctcattg ggacaatact agctcaaaaa ctatttgttt taccccgcgg
    27661 gatgaaacgt ttcgtaaaat tctttgagat tctctcggta ccatcgatca tctggggatt
    27721 gatctacagt tcatttttcg gacaggcatt accaaaagaa ctatttggta tccgcttgcc
    27781 tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt
    27841 cggtctgata cagatcttgg ttggattatt cgtggcagca aaggaaaata tcaggaggaa
    27901 agattacttg agtgctgtca gtgatggttt tgcctggcaa gggatcctga ttgggatcgt
    27961 tattgcattg attggtgtca tgttgataaa aaatacagca tttgtttatc ttggcgcggg
    28021 gctatcgatc attgctgctt tatgtatctt aatcgtgccg attatccaat cgccgtctaa
    28081 agtcaagggt gctgccaaag gagcgtataa tctttacgga ttgactggtt atatcggtga
    28141 tttagtcagt tatacacgtt tgatggcact aggtatttca ggcggaagta ttggcgcggc
    28201 attcaatatg ctagttgctt tcatgccgcc agccgctcga ttcagtgtag ggatcctttt
    28261 gatcatcgct ttacacgcac tgaatatgtt tttgacactg ttaagtgcct atgtccatgg
    28321 tgcacgattg cagtatgtcg aattttttgg aaaattttat acaggaggcg gacgtgcctt
    28381 tgacccgctg aaaacggcgg aaaaatacgt caacatcaat cataagaaaa aaaacaataa
    28441 ataattggag gatttttata caatgatgga ttacttaatt agtcaaaatg gtggaatggt
    28501 atttgcagta ttagcgatgg caacagcgac gattttttca gggattggtt cagctaaagg
    28561 tgttggaatg acaggtgagg cagcagcagc gttaacaaca agtcaacccg aaaaattcgg
    28621 acaggctttg attctgcaat tgcttccagg tacgcaaggg ttgtatggat tcgttatcgc
    28681 ctttttgatt tttatcaact taggaaatga tatgtcagtt gtacaaggat tgaactttct
    28741 aggcgcttct ctgccgattg cttttacagg tttgttttca ggtatcgccc aagggaaagt
    28801 tgcagcagca ggtatccaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat
    28861 ttttgctgcg atggttgaaa catatgcgat tttaggtttc gttatttcct tcctattagt
    28921 attgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct
    28981 cagatgaatg aagcagcaga gcaagaaaga gcggcgttag agcaagaaga gcgaatgaag
    29041 atcgatcaaa actttgaaca aaaacgtacg caaatcgaaa cagaacatca aaaacaaaaa
    29101 gaaaaacaaa tcgaattact tgaaaaaaaa taccgtcagc ttaggaatcg ccaacaagta
    29161 gaagtacgac aagaaaattt gaatgcgaag caggaatttt tacgacgatt gtttgcagat
    29221 gcaatagctg aaatggagaa ttgggatgaa tctgaacaaa tccagtttat caaaaattca
    29281 ttgtattcat tacctttgac aggcaaagta acattcattg ctggagaaaa atcagcagct
    29341 tatctttctc agacattgct ggatgaatgg aacaatgaac ttccctttat gatggtttta
    29401 agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat
    29461 aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc
    29521 aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga
    29581 ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa
    29641 attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg
    29701 acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct
    29761 ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgctatactt
    29821 tccataatct gaaggttttg acaaaagccg agatgacaaa tcaaaatctg gaccatttgt
    29881 atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt
    29941 caaatgaact ccctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag
    30001 agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc
    30061 gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg
    30121 acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca
    30181 tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag
    30241 ataaagatat ggaagattac acgaattttc tattagagac agattatgct gattttctgc
    30301 gccctgtcat ccgtgaggga aatcttgatc tcgtaaagct tgaacagttg aaagatgatt
    30361 atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag
    30421 cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa
    30481 gaagccattt cgaaccagaa acaatcagaa agcgggtgag acaagtctat gacgcataag
    30541 atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt
    30601 cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag
    30661 tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt
    30721 tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg
    30781 atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag
    30841 tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt
    30901 gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta
    30961 ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa
    31021 gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt
    31081 gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc
    31141 tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac
    31201 catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga
    31261 gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat
    31321 gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta
    31381 tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta
    31441 agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag
    31501 cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct
    31561 tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg
    31621 gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc
    31681 cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct
    31741 aatacatcaa acatgccagt ggcagctcga gaagcttcta tctatacggg aatcacaatt
    31801 gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga
    31861 tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt
    31921 tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt
    31981 gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct
    32041 ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg
    32101 ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc
    32161 tattctcttt attcaacaga agtagggaag tatatggatc atatccttca agctgactgg
    32221 gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc
    32281 gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca
    32341 aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact
    32401 tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc
    32461 cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt
    32521 gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt
    32581 attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat
    32641 taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc
    32701 tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg
    32761 acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg
    32821 tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc
    32881 ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga
    32941 gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga
    33001 ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt
    33061 tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc
    33121 tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc
    33181 tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg
    33241 cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat
    33301 cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca
    33361 tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc
    33421 agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc
    33481 tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc
    33541 gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat
    33601 tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat
    33661 tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag
    33721 agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc
    33781 aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc
    33841 gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc
    33901 gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt
    33961 aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat
    34021 ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac
    34081 aactgccaca agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt
    34141 catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga
    34201 agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt
    34261 atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt
    34321 aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata
    34381 cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct
    34441 tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat
    34501 cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga
    34561 aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt
    34621 gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa
    34681 acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca
    34741 ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt
    34801 aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt
    34861 gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg
    34921 atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt
    34981 gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat
    35041 ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtt
    35101 atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg
    35161 aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat
    35221 ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct
    35281 gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat
    35341 cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta
    35401 ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg
    35461 aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga
    35521 tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg
    35581 atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt
    35641 gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc
    35701 cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg
    35761 ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc
    35821 atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga
    35881 ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga
    35941 cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca
    36001 aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc
    36061 tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt
    36121 gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct
    36181 tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca
    36241 tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta
    36301 gaggataggg aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa
    36361 tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca
    36421 ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa
    36481 agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta
    36541 tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc
    36601 acatttatat tataaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga
    36661 taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg
    36721 ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat
    36781 gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc
    36841 tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag
    36901 acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc
    36961 tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta
    37021 gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg
    37081 atagccaatc cattttgaac accggaaaca atagatagag attctaagga cggaatgact
    37141 ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct
    37201 tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca
    37261 acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt
    37321 acttcattgg tagagatggt gatggactca aagacattgg gagaattaaa tgtgccactt
    37381 ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt gccaacggta
    37441 gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc
    37501 tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga
    37561 cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata
    37621 ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat
    37681 tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa
    37741 gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat
    37801 tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa
    37861 ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca
    37921 aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag
    37981 attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc
    38041 acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat
    38101 gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt
    38161 tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat
    38221 acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca
    38281 gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca
    38341 tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact
    38401 cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat
    38461 tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta
    38521 gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat
    38581 aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt
    38641 tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt
    38701 acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc
    38761 tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg
    38821 aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc
    38881 gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt
    38941 acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa
    39001 gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat
    39061 tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt
    39121 tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta
    39181 tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact
    39241 ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag
    39301 cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt
    39361 atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa
    39421 taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg
    39481 aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag
    39541 acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt
    39601 ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta
    39661 taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag
    39721 tcagtaatct caagaccggc agctttatgt tgctcgtgta atttttctag atcatcagct
    39781 gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa
    39841 gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt
    39901 tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt
    39961 gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc
    40021 cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt
    40081 catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc
    40141 atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca
    40201 gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct
    40261 ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt
    40321 gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat
    40381 gagtattttt actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt
    40441 gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg
    40501 agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga
    40561 tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt
    40621 tatttattac agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga
    40681 agtgatgaca tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag
    40741 accagcatat tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc
    40801 ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt
    40861 atttagtact tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt
    40921 ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt
    40981 gacgatgttc atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac
    41041 taaacttgct tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt
    41101 gtcatcgatc gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa
    41161 atcttcatgt gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag
    41221 tgccccgtta taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg
    41281 ttcctggaca cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac
    41341 agcagcggca atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt
    41401 accatcgatg tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt
    41461 tatttcaatc aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa
    41521 taaatcataa tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc
    41581 atctccttgt tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat
    41641 caaagttccg tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg
    41701 ataaaaatca taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata
    41761 tccgaccttt tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga
    41821 aaaagtagca gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat
    41881 aggatcagca gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt
    41941 gaggtattct tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag
    42001 ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa
    42061 aattacttcc ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg
    42121 gctaacgata taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc
    42181 ataaggacga atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt
    42241 gaaataagca tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat
    42301 ttggacaact tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa
    42361 accttcttct actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc
    42421 tacagtgatg gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc
    42481 atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca
    42541 gcctccgttg cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct
    42601 tgaaatctca taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt
    42661 gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat
    42721 caaatctaag attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac
    42781 tttttccata ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt
    42841 tattacgttg ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt
    42901 ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg
    42961 caaatgcttc tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg
    43021 ccattgtagc aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag
    43081 attgataaaa tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt
    43141 acatcataac agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc
    43201 catggatgct taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta
    43261 tcggtgcctt tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta
    43321 tggttatttt ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat
    43381 ctgatacgat ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca
    43441 gtgtttggat cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg
    43501 tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta
    43561 atatatctgt taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc
    43621 gaatcgcagg gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat
    43681 actctaaaat caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt
    43741 tgacaaaaca aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat
    43801 ggaattatca caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg
    43861 ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct
    43921 gtacatacgt tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa
    43981 attagttcca gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa
    44041 atttggctat aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat
    44101 tgctactttt acgaaagaac tgcatgttag tttgaaagac cgtcccacag gagggaaggt
    44161 tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt
    44221 ggattttaca gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat
    44281 aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa
    44341 caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt
    44401 catggatttt tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga
    44461 aattcgtgat tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc
    44521 attaggggat aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga
    44581 agtaaaccat ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa
    44641 agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga
    44701 agatacagat ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga
    44761 tgaagaaatc gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga
    44821 tgatgaagaa gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga
    44881 tgatgatgaa gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt
    44941 ttcttacgag aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga
    45001 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
    45061 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
    45121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
    45181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
    45241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
    45301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
    45361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
    45421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
    45481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
    45541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
    45601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
    45661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
    45721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
    45781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
    45841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
    45901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
    45961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
    46021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
    46081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
    46141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
    46201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
    46261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
    46321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
    46381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
    46441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
    46501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
    46561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
    46621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
    46681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
    46741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
    46801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
    46861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
    46921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
    46981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
    47041 gaaatcgtga agcagacagc tgaagcaggg cgcgagattg gcaatcacac atacgatcat
    47101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
    47161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
    47221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
    47281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
    47341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
    47401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
    47461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
    47521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
    47581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
    47641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
    47701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
    47761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
    47821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
    47881 gaaactaacc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
    47941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
    48001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
    48061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
    48121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
    48181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
    48241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
    48301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
    48361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
    48421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
    48481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
    48541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
    48601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
    48661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
    48721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
    48781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
    48841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
    48901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
    48961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
    49021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
    49081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
    49141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
    49201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
    49261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
    49321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgttgg
    49381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
    49441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
    49501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
    49561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
    49621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
    49681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
    49741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
    49801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
    49861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
    49921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
    49981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
    50041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
    50101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
    50161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
    50221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
    50281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
    50341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
    50401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
    50461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
    50521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
    50581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
    50641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
    50701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
    50761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
    50821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
    50881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
    50941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
    51001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
    51061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
    51121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
    51181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
    51241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
    51301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
    51361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
    51421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
    51481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
    51541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
    51601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
    51661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
    51721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
    51781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
    51841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
    51901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
    51961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
    52021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
    52081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
    52141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
    52201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
    52261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
    52321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
    52381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
    52441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
    52501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
    52561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
    52621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
    52681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
    52741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
    52801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
    52861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
    52921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
    52981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
    53041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
    53101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
    53161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
    53221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
    53281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
    53341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
    53401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
    53461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
    53521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
    53581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
    53641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
    53701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
    53761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
    53821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
    53881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
    53941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
    54001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
    54061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
    54121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
    54181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
    54241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
    54301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
    54361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
    54421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
    54481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
    54541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
    54601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
    54661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
    54721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
    54781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
    54841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
    54901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
    54961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    55021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    55081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga ga
//
LOCUS       DAFFQY010000006        54448 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFFQY010000006 DAFFQY010000000
VERSION     DAFFQY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.006"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            12..590
                     /locus_tag="LRB02_000298"
     CDS             12..590
                     /locus_tag="LRB02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658536.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="LRB02_000299"
     tRNA            complement(830..913)
                     /locus_tag="LRB02_000299"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            968..1147
                     /locus_tag="LRB02_000300"
     CDS             968..1147
                     /locus_tag="LRB02_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658537.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1296..2513
                     /locus_tag="LRB02_000301"
     CDS             1296..2513
                     /locus_tag="LRB02_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4658538.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="LRB02_000302"
     CDS             2697..3533
                     /locus_tag="LRB02_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4658539.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="LRB02_000303"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="LRB02_000303"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL4658540.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="LRB02_000304"
     CDS             4978..6036
                     /locus_tag="LRB02_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL4658541.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6040..7074
                     /locus_tag="LRB02_000305"
     CDS             6040..7074
                     /locus_tag="LRB02_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL4658542.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7176..7892)
                     /locus_tag="LRB02_000306"
     CDS             complement(7176..7892)
                     /locus_tag="LRB02_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4658543.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="LRB02_000307"
     CDS             complement(7861..8646)
                     /locus_tag="LRB02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL4658544.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="LRB02_000308"
     CDS             complement(8637..9386)
                     /locus_tag="LRB02_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL4658545.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="LRB02_000309"
     CDS             9684..10208
                     /locus_tag="LRB02_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL4658546.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="LRB02_000310"
     CDS             10224..11723
                     /locus_tag="LRB02_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL4658547.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="LRB02_000311"
     CDS             complement(11811..12413)
                     /locus_tag="LRB02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4658548.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="LRB02_000312"
     CDS             12517..13473
                     /locus_tag="LRB02_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL4658549.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="LRB02_000313"
     CDS             complement(13778..14734)
                     /locus_tag="LRB02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4658550.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="LRB02_000314"
     CDS             14908..15468
                     /locus_tag="LRB02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658551.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16285
                     /locus_tag="LRB02_000315"
     CDS             15461..16285
                     /locus_tag="LRB02_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL4658552.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16295..16912
                     /locus_tag="LRB02_000316"
     CDS             16295..16912
                     /locus_tag="LRB02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL4658553.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="LRB02_000317"
     CDS             16930..17613
                     /locus_tag="LRB02_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL4658554.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="LRB02_000318"
     CDS             18296..19579
                     /locus_tag="LRB02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4658555.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="LRB02_000319"
     CDS             19595..20482
                     /locus_tag="LRB02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658556.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="LRB02_000320"
     CDS             20499..21335
                     /locus_tag="LRB02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4658557.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="LRB02_000321"
     CDS             21338..21961
                     /locus_tag="LRB02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658558.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="LRB02_000322"
     CDS             21958..23250
                     /locus_tag="LRB02_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4658559.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="LRB02_000323"
     CDS             23822..24403
                     /locus_tag="LRB02_000323"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL4658560.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="LRB02_000324"
     CDS             24406..25713
                     /locus_tag="LRB02_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL4658561.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="LRB02_000325"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="LRB02_000325"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL4658562.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26610..27734
                     /gene="purK"
                     /locus_tag="LRB02_000326"
     CDS             26610..27734
                     /gene="purK"
                     /locus_tag="LRB02_000326"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL4658563.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="LRB02_000327"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="LRB02_000327"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL4658564.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="LRB02_000328"
     CDS             complement(29222..29599)
                     /locus_tag="LRB02_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658565.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="LRB02_000329"
     CDS             29855..30583
                     /locus_tag="LRB02_000329"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL4658566.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="LRB02_000330"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="LRB02_000330"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL4658567.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="LRB02_000331"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="LRB02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL4658568.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="LRB02_000332"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="LRB02_000332"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL4658569.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="LRB02_000333"
     CDS             33718..35157
                     /locus_tag="LRB02_000333"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL4658570.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35180..36232
                     /locus_tag="LRB02_000334"
     CDS             35180..36232
                     /locus_tag="LRB02_000334"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL4658571.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="LRB02_000335"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="LRB02_000335"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL4658572.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="LRB02_000336"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="LRB02_000336"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL4658573.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="LRB02_000337"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="LRB02_000337"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL4658574.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="LRB02_000338"
     CDS             39751..40665
                     /locus_tag="LRB02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4658575.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="LRB02_000339"
     CDS             complement(40798..42363)
                     /locus_tag="LRB02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4658576.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="LRB02_000340"
     CDS             complement(42356..43807)
                     /locus_tag="LRB02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL4658577.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="LRB02_000341"
     CDS             complement(43821..44837)
                     /locus_tag="LRB02_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4658578.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="LRB02_000342"
     CDS             45057..46145
                     /locus_tag="LRB02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL4658579.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="LRB02_000343"
     CDS             46199..47725
                     /locus_tag="LRB02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL4658580.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="LRB02_000344"
     CDS             47727..48932
                     /locus_tag="LRB02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658581.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="LRB02_000345"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="LRB02_000345"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL4658582.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="LRB02_000346"
     CDS             50430..51854
                     /locus_tag="LRB02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL4658583.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="LRB02_000347"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="LRB02_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL4658584.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="LRB02_000348"
     CDS             53427..54404
                     /locus_tag="LRB02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL4658585.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17403 a         9316 c        11921 g        15808 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtatagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcaatg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAFFQY010000007        51580 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFFQY010000007 DAFFQY010000000
VERSION     DAFFQY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.007"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            290..1135
                     /locus_tag="LRB02_000349"
     CDS             290..1135
                     /locus_tag="LRB02_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4658586.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="LRB02_000350"
     CDS             1416..2636
                     /locus_tag="LRB02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL4658587.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="LRB02_000351"
     CDS             2633..5353
                     /locus_tag="LRB02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4658588.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="LRB02_000352"
     CDS             5337..6281
                     /locus_tag="LRB02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4658589.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="LRB02_000353"
     CDS             complement(6350..7360)
                     /locus_tag="LRB02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4658590.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="LRB02_000354"
     CDS             complement(7540..7884)
                     /locus_tag="LRB02_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL4658591.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="LRB02_000355"
     CDS             complement(7877..8311)
                     /locus_tag="LRB02_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL4658592.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="LRB02_000356"
     CDS             8449..9186
                     /locus_tag="LRB02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658593.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="LRB02_000357"
     CDS             9179..10390
                     /locus_tag="LRB02_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658594.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="LRB02_000358"
     CDS             10594..11376
                     /locus_tag="LRB02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL4658595.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="LRB02_000359"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="LRB02_000359"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL4658596.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LRB02_000360"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LRB02_000360"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL4658597.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="LRB02_000361"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="LRB02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL4658598.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="LRB02_000362"
     CDS             14978..16027
                     /locus_tag="LRB02_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL4658599.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="LRB02_000363"
     CDS             16024..16302
                     /locus_tag="LRB02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL4658600.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="LRB02_000364"
     CDS             16277..16747
                     /locus_tag="LRB02_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL4658601.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="LRB02_000365"
     CDS             16764..17066
                     /locus_tag="LRB02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658602.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="LRB02_000366"
     CDS             17083..17454
                     /locus_tag="LRB02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL4658603.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="LRB02_000367"
     CDS             17426..17785
                     /locus_tag="LRB02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658604.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="LRB02_000368"
     CDS             17957..18964
                     /locus_tag="LRB02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4658605.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="LRB02_000369"
     CDS             18989..20173
                     /locus_tag="LRB02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL4658606.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="LRB02_000370"
     CDS             complement(20610..22193)
                     /locus_tag="LRB02_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4658607.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="LRB02_000371"
     CDS             complement(22771..22914)
                     /locus_tag="LRB02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL4658608.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="LRB02_000372"
     CDS             complement(23136..23615)
                     /locus_tag="LRB02_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4658609.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="LRB02_000373"
     CDS             complement(23688..23972)
                     /locus_tag="LRB02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658610.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="LRB02_000374"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LRB02_000374"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="LRB02_000375"
     CDS             complement(24316..25602)
                     /locus_tag="LRB02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL4658611.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="LRB02_000376"
     CDS             25740..26411
                     /locus_tag="LRB02_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL4658612.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="LRB02_000377"
     CDS             26377..26865
                     /locus_tag="LRB02_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL4658613.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="LRB02_000378"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="LRB02_000378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL4658614.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="LRB02_000379"
     CDS             27826..28590
                     /locus_tag="LRB02_000379"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL4658615.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEEKQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="LRB02_000380"
     CDS             28823..31036
                     /locus_tag="LRB02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL4658616.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="LRB02_000381"
     CDS             31047..31493
                     /locus_tag="LRB02_000381"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL4658617.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="LRB02_000382"
     CDS             31664..32923
                     /locus_tag="LRB02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL4658618.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="LRB02_000383"
     CDS             complement(33012..33959)
                     /locus_tag="LRB02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4658619.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="LRB02_000384"
     CDS             34353..35645
                     /locus_tag="LRB02_000384"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL4658620.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="LRB02_000385"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="LRB02_000385"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL4658621.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="LRB02_000386"
     CDS             37538..38413
                     /locus_tag="LRB02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4658622.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="LRB02_000387"
     CDS             38679..40562
                     /locus_tag="LRB02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL4658623.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="LRB02_000388"
     CDS             complement(40637..40921)
                     /locus_tag="LRB02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4658624.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="LRB02_000389"
     CDS             complement(40911..41645)
                     /locus_tag="LRB02_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL4658625.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="LRB02_000390"
     CDS             41774..42397
                     /locus_tag="LRB02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL4658626.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="LRB02_000391"
     CDS             42589..43710
                     /locus_tag="LRB02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL4658627.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="LRB02_000392"
     CDS             43717..46845
                     /locus_tag="LRB02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL4658628.1"
                     /translation="MKPKKLQLKNFGLFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="LRB02_000393"
     CDS             46864..50058
                     /locus_tag="LRB02_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL4658629.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="LRB02_000394"
     CDS             complement(50204..50911)
                     /locus_tag="LRB02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4658630.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16919 a         8978 c        10674 g        15009 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacgaagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaaacaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtctttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgttta
    51241 tagtaaattt tatttagtaa tcattgcttt aaaatactac gcttatataa cagagaaaat
    51301 aagcgttata ataaagaaca gtattattta aaatgacaga aaacattact attaaagaaa
    51361 taaaaaacat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    51421 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    51481 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
    51541 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt
//
LOCUS       DAFFQY010000008        51533 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFFQY010000008 DAFFQY010000000
VERSION     DAFFQY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.008"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            273..389
                     /locus_tag="LRB02_000395"
     CDS             273..389
                     /locus_tag="LRB02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL4658631.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            1317..1655
                     /locus_tag="LRB02_000396"
     CDS             1317..1655
                     /locus_tag="LRB02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658632.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            1749..1979
                     /locus_tag="LRB02_000397"
     CDS             1749..1979
                     /locus_tag="LRB02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658633.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            2379..2750
                     /locus_tag="LRB02_000398"
     CDS             2379..2750
                     /locus_tag="LRB02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL4658634.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(3024..3716)
                     /locus_tag="LRB02_000399"
     CDS             complement(3024..3716)
                     /locus_tag="LRB02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4658635.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(3719..4504)
                     /locus_tag="LRB02_000400"
     CDS             complement(3719..4504)
                     /locus_tag="LRB02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4658636.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            4741..6141
                     /gene="lacG"
                     /locus_tag="LRB02_000401"
     CDS             4741..6141
                     /gene="lacG"
                     /locus_tag="LRB02_000401"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL4658637.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6166..6483
                     /locus_tag="LRB02_000402"
     CDS             6166..6483
                     /locus_tag="LRB02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4658638.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            6496..8170
                     /locus_tag="LRB02_000403"
                     /pseudo
     CDS             6496..8170
                     /locus_tag="LRB02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
     gene            8184..9815
                     /locus_tag="LRB02_000404"
     CDS             8184..9815
                     /locus_tag="LRB02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL4658639.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(10306..11871)
                     /gene="guaA"
                     /locus_tag="LRB02_000405"
     CDS             complement(10306..11871)
                     /gene="guaA"
                     /locus_tag="LRB02_000405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL4658640.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(11930..12853)
                     /locus_tag="LRB02_000406"
     CDS             complement(11930..12853)
                     /locus_tag="LRB02_000406"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL4658641.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13049..13654
                     /locus_tag="LRB02_000407"
     CDS             13049..13654
                     /locus_tag="LRB02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4658642.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            13962..14978
                     /locus_tag="LRB02_000408"
     CDS             13962..14978
                     /locus_tag="LRB02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL4658643.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            15119..16420
                     /locus_tag="LRB02_000409"
     CDS             15119..16420
                     /locus_tag="LRB02_000409"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL4658644.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            16466..17128
                     /gene="deoC"
                     /locus_tag="LRB02_000410"
     CDS             16466..17128
                     /gene="deoC"
                     /locus_tag="LRB02_000410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL4658645.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            17149..17547
                     /locus_tag="LRB02_000411"
     CDS             17149..17547
                     /locus_tag="LRB02_000411"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL4658646.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            17613..18695
                     /locus_tag="LRB02_000412"
     CDS             17613..18695
                     /locus_tag="LRB02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4658647.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            18907..20469
                     /locus_tag="LRB02_000413"
     CDS             18907..20469
                     /locus_tag="LRB02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658648.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            20462..21595
                     /locus_tag="LRB02_000414"
     CDS             20462..21595
                     /locus_tag="LRB02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658649.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            21595..22551
                     /locus_tag="LRB02_000415"
     CDS             21595..22551
                     /locus_tag="LRB02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658650.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            22650..23816
                     /gene="deoB"
                     /locus_tag="LRB02_000416"
     CDS             22650..23816
                     /gene="deoB"
                     /locus_tag="LRB02_000416"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL4658651.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            23840..24658
                     /locus_tag="LRB02_000417"
     CDS             23840..24658
                     /locus_tag="LRB02_000417"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL4658652.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            24675..25382
                     /gene="deoD"
                     /locus_tag="LRB02_000418"
     CDS             24675..25382
                     /gene="deoD"
                     /locus_tag="LRB02_000418"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL4658653.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(25680..26366)
                     /gene="gpmA"
                     /locus_tag="LRB02_000419"
     CDS             complement(25680..26366)
                     /gene="gpmA"
                     /locus_tag="LRB02_000419"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL4658654.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(26538..27119)
                     /locus_tag="LRB02_000420"
     CDS             complement(26538..27119)
                     /locus_tag="LRB02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4658655.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(27252..27935)
                     /gene="rpiA"
                     /locus_tag="LRB02_000421"
     CDS             complement(27252..27935)
                     /gene="rpiA"
                     /locus_tag="LRB02_000421"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL4658656.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            28254..28667
                     /gene="rpsL"
                     /locus_tag="LRB02_000422"
     CDS             28254..28667
                     /gene="rpsL"
                     /locus_tag="LRB02_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL4658657.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            28751..29221
                     /gene="rpsG"
                     /locus_tag="LRB02_000423"
     CDS             28751..29221
                     /gene="rpsG"
                     /locus_tag="LRB02_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL4658658.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            29315..31402
                     /gene="fusA"
                     /locus_tag="LRB02_000424"
     CDS             29315..31402
                     /gene="fusA"
                     /locus_tag="LRB02_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL4658659.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            31574..32761
                     /gene="tuf"
                     /locus_tag="LRB02_000425"
     CDS             31574..32761
                     /gene="tuf"
                     /locus_tag="LRB02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL4658660.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            33168..33476
                     /gene="rpsJ"
                     /locus_tag="LRB02_000426"
     CDS             33168..33476
                     /gene="rpsJ"
                     /locus_tag="LRB02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL4658661.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            33507..34136
                     /gene="rplC"
                     /locus_tag="LRB02_000427"
     CDS             33507..34136
                     /gene="rplC"
                     /locus_tag="LRB02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL4658662.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            34164..34787
                     /gene="rplD"
                     /locus_tag="LRB02_000428"
     CDS             34164..34787
                     /gene="rplD"
                     /locus_tag="LRB02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL4658663.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            34787..35077
                     /gene="rplW"
                     /locus_tag="LRB02_000429"
     CDS             34787..35077
                     /gene="rplW"
                     /locus_tag="LRB02_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL4658664.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            35116..35949
                     /gene="rplB"
                     /locus_tag="LRB02_000430"
     CDS             35116..35949
                     /gene="rplB"
                     /locus_tag="LRB02_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL4658665.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            35992..36270
                     /gene="rpsS"
                     /locus_tag="LRB02_000431"
     CDS             35992..36270
                     /gene="rpsS"
                     /locus_tag="LRB02_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL4658666.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            36293..36640
                     /gene="rplV"
                     /locus_tag="LRB02_000432"
     CDS             36293..36640
                     /gene="rplV"
                     /locus_tag="LRB02_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL4658667.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            36654..37310
                     /gene="rpsC"
                     /locus_tag="LRB02_000433"
     CDS             36654..37310
                     /gene="rpsC"
                     /locus_tag="LRB02_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL4658668.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            37313..37747
                     /gene="rplP"
                     /locus_tag="LRB02_000434"
     CDS             37313..37747
                     /gene="rplP"
                     /locus_tag="LRB02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL4658669.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            37737..37925
                     /gene="rpmC"
                     /locus_tag="LRB02_000435"
     CDS             37737..37925
                     /gene="rpmC"
                     /locus_tag="LRB02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL4658670.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            37950..38216
                     /gene="rpsQ"
                     /locus_tag="LRB02_000436"
     CDS             37950..38216
                     /gene="rpsQ"
                     /locus_tag="LRB02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL4658671.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            38274..38642
                     /gene="rplN"
                     /locus_tag="LRB02_000437"
     CDS             38274..38642
                     /gene="rplN"
                     /locus_tag="LRB02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL4658672.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            38679..38987
                     /gene="rplX"
                     /locus_tag="LRB02_000438"
     CDS             38679..38987
                     /gene="rplX"
                     /locus_tag="LRB02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL4658673.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            39015..39554
                     /gene="rplE"
                     /locus_tag="LRB02_000439"
     CDS             39015..39554
                     /gene="rplE"
                     /locus_tag="LRB02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL4658674.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            39573..39758
                     /locus_tag="LRB02_000440"
     CDS             39573..39758
                     /locus_tag="LRB02_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL4658675.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            39794..40192
                     /gene="rpsH"
                     /locus_tag="LRB02_000441"
     CDS             39794..40192
                     /gene="rpsH"
                     /locus_tag="LRB02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL4658676.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            40224..40760
                     /gene="rplF"
                     /locus_tag="LRB02_000442"
     CDS             40224..40760
                     /gene="rplF"
                     /locus_tag="LRB02_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL4658677.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            40921..41277
                     /gene="rplR"
                     /locus_tag="LRB02_000443"
     CDS             40921..41277
                     /gene="rplR"
                     /locus_tag="LRB02_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL4658678.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            41298..41798
                     /gene="rpsE"
                     /locus_tag="LRB02_000444"
     CDS             41298..41798
                     /gene="rpsE"
                     /locus_tag="LRB02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL4658679.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            41813..41992
                     /gene="rpmD"
                     /locus_tag="LRB02_000445"
     CDS             41813..41992
                     /gene="rpmD"
                     /locus_tag="LRB02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL4658680.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            42036..42476
                     /gene="rplO"
                     /locus_tag="LRB02_000446"
     CDS             42036..42476
                     /gene="rplO"
                     /locus_tag="LRB02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL4658681.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            42476..43771
                     /gene="secY"
                     /locus_tag="LRB02_000447"
     CDS             42476..43771
                     /gene="secY"
                     /locus_tag="LRB02_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL4658682.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            43833..44480
                     /locus_tag="LRB02_000448"
     CDS             43833..44480
                     /locus_tag="LRB02_000448"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL4658683.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            44671..44889
                     /gene="infA"
                     /locus_tag="LRB02_000449"
     CDS             44671..44889
                     /gene="infA"
                     /locus_tag="LRB02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL4658684.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            44922..45038
                     /gene="rpmJ"
                     /locus_tag="LRB02_000450"
     CDS             44922..45038
                     /gene="rpmJ"
                     /locus_tag="LRB02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL4658685.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            45056..45421
                     /gene="rpsM"
                     /locus_tag="LRB02_000451"
     CDS             45056..45421
                     /gene="rpsM"
                     /locus_tag="LRB02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL4658686.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            45449..45838
                     /gene="rpsK"
                     /locus_tag="LRB02_000452"
     CDS             45449..45838
                     /gene="rpsK"
                     /locus_tag="LRB02_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL4658687.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            45918..46856
                     /locus_tag="LRB02_000453"
     CDS             45918..46856
                     /locus_tag="LRB02_000453"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL4658688.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            46884..47264
                     /gene="rplQ"
                     /locus_tag="LRB02_000454"
     CDS             46884..47264
                     /gene="rplQ"
                     /locus_tag="LRB02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL4658689.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            47536..48375
                     /locus_tag="LRB02_000455"
     CDS             47536..48375
                     /locus_tag="LRB02_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4658690.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            48351..49220
                     /locus_tag="LRB02_000456"
     CDS             48351..49220
                     /locus_tag="LRB02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4658691.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            49210..50007
                     /locus_tag="LRB02_000457"
     CDS             49210..50007
                     /locus_tag="LRB02_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL4658692.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            50210..50956
                     /gene="truA"
                     /locus_tag="LRB02_000458"
     CDS             50210..50956
                     /gene="truA"
                     /locus_tag="LRB02_000458"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL4658693.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        16415 a         9292 c        11204 g        14622 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
      481 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
      541 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
      601 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
      661 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
      721 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
      781 cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat
      841 atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc
      901 tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
      961 aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat
     1021 aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa
     1081 ttttgtgaga atcactatta caagtatctt taataaaatt aatggttaag gaggaactta
     1141 aagtagaatg taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca
     1201 ggaataagtt tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt
     1261 atactttgtt gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga
     1321 ctcaaccagt ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga
     1381 aatatcagga aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg
     1441 atggaaacca aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa
     1501 tagtaaaaaa tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa
     1561 cgtatattta tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa
     1621 ttgctactga taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat
     1681 agaaattgat gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc
     1741 tattcgatat gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag
     1801 ttagagaaga tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc
     1861 cgacatttac agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa
     1921 gtggaatgtc tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag
     1981 ggagttctta ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg
     2041 ctgttaatat tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact
     2101 tttgtatatt tagtaagtag ctacacactt ctatataaat tcagaagttc ttctagagtt
     2161 tctaaagttt gtttcttaat tagcttattg attctataat atttttgatt tatataggaa
     2221 attaaaaata ttatagaatc aacactcaga gaacgggttg gtttttaata gatttaataa
     2281 gtactgataa tgaaatttct cctggttggg aaaacgaagt tggttaccct gtagaagaat
     2341 tttttcaaga ttactttgcg gagtatgccg ataggataat gagagattac gtaacagata
     2401 aaactcaaga agtcatcaat caatcccagt cattaaatga attatctgat aaggcgaaag
     2461 aagaagcagt tgccattatt tcttcaggga ttagtgacta tattgatatg agtggtgaaa
     2521 tcaattatgg cgataaagac aaaattgtac gcatgataaa agcttttatc aaaacttttg
     2581 atgtgaaggc agatcttaag tttaatgata cttacctagt aggaaaatta attaatatcc
     2641 ttgggacaga gaaagatacg gaaacgttta tttcctcttt gtctatgttg tttacagata
     2701 agcatttttc aactcagctt ggtggagagg aactagtgga aatcttttaa ccattacgcc
     2761 cagcattaat aaaagtatat caaaaaagta ctcctgatga tttttaagat tggtttgaga
     2821 aataatgtga aaacactatt tttaatgtta attatagata gagatgaacg cgtcatatgg
     2881 ttaaaagaat gaaaaagtca aagatacact gctatatttc actaaggata tagcagtgta
     2941 tctttcatgt cgtttcactg gcagtttttt ataaaaggag gtgtgcattt cagttgcttt
     3001 tctttggtag ttagttgaat cgtttaagaa ttaacggatg tggcagacag gtgctctgtc
     3061 caaaaaccag taaaatcttt gatggttcca tctttattta gctgtgcctg ttcaaatact
     3121 gttttgtttt taccgaatgg atcaattgca ataattgttc cgatccccgc acgtttggca
     3181 gcaagtaatc ctgagtaagc atcttcgata acaagacaat cagccggagc taagctcagt
     3241 ttgtctgccg ctttaagaaa aatatccggt tgcggtttcc cagggaaaga gccgtcgtca
     3301 tagacaatct tgtcccagtc aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca
     3361 acattctctt tgacagtagc cgtggcaatc gttaatggaa tctgttggtc agtcagctgg
     3421 tcaagagtcg cttcaagccc ttttgtcaac tgaagctcgt tttcattttg caagcaaagc
     3481 atacgataat agctttcttt ttcttctgac aatacggcga tctcagaggc ggtcaaatgc
     3541 tctgaaatga aatgtttcaa aatctccgta ttcgtccgac catgaatatt ttttaaaata
     3601 tcctcgtctg ataaaccatc ttttgtatga tcatgtatca tatgcagcca agcagcttca
     3661 tgcagatgag agtccaaaaa cattgttccg ttgaaatcaa atataatccc tttcatagtt
     3721 acgctcctat agtggtattt tcggttgaat cagcaatttg cagatcgaca tattgtgcat
     3781 agtgctcttg aacctcccgc gagatctggc gatccgtaat gagcagatcg aaatcatgca
     3841 aattataaaa cacataaaaa tcatctcgat tgaatttttt attatcaacg agtagatatt
     3901 tcttacggga attatttaga gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag
     3961 agatcgcttc gttgtgaacg ccatttgaac tgataaatgc ttttgtaaac tttagtcttc
     4021 ttaaattgtc gttggtgatc ggtccgacaa atgcgccggt atttttccgg aaatcaccgc
     4081 caatcagtag aatttctggt ccatctgctt gagcaagaat atcaaaaact ggataattat
     4141 ttgtaatgat ccgaattttc ttgttcagta aatgcgtcgc caatagttcg attgttgtac
     4201 ctggtcctaa gaaaatcgtt tctccctcgt gaatcaagga ggctgctgtg gcagcaattt
     4261 cttttttttc ttgaatctgc agagtggctt tttcagtgtg agaaagttct tgatctaaag
     4321 tgtaggaaag actttgagcc cctccatgga cacgcagcag cttgcccgat ttttctaatt
     4381 catctaaatc tctgcgaacg gtcatatcag aaacatctaa ttgattgatg atttcattaa
     4441 cggtaaggat tcctttttta ttgaccattt ctacgatagt aagcaatctt tcttttttca
     4501 acattgtctt cacctctaat ttaaggataa tctatcatat aaacggacaa cagtcaatct
     4561 aattcgaaca aaagtaaaca tttagggtgg tttttatgtt tgttacaata gattttattg
     4621 ctgttttctg gtttttatga ttatttgtgt ttgtttttgt tgacaaatgt ttcggatgcg
     4681 tttacaatgc aatcagttag aaagagcgaa aactgattat agatttgagg agagatgtac
     4741 atgcttaaat taccagaaga ttttattttt ggcggagcca cagcagccta tcaggttgaa
     4801 ggagccacta aggaaggtgg caaaggcgct gttgcttggg atgacttttt agaggaacaa
     4861 gggcgattca gtcccgatcc ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa
     4921 ttgtgtgaac gttttggtgt aaatggtcta agattatcca ttgcttggag ccgcattttt
     4981 cctgatggag ctgggaaacc aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa
     5041 gaatgtaaga aaagaaacgt aacgccgttt gtgacgttgc atcattttga tacccctaaa
     5101 cgtttgtttg accatggaga ttttttgaat cgagaaacaa tagaagcatt tgtttcctat
     5161 gccatttttt gttttcatga attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc
     5221 tatccagttg caaccaatca atatttgtta ggtgttttcc caccaggaat caaatatgat
     5281 tttacaaaga tcgtcgcctg tcttcataat atgatggttg ctcatgcccg tgtagtgaac
     5341 tatttcaaag aaaacgaact tccgggtgaa attggtgtag ttcactcatt agagacgaaa
     5401 tatgcggcaa cagacgcgcc ggaagataag catgctgcct ttttagacga tgcattgtcg
     5461 atccgtttct tattagatgc tacctattta ggctattatt caactgaaac cttgactgct
     5521 cttgatgaga tatgtgaagc aaatcaggcc agctatcatt ttcctgaaga agatttcgtt
     5581 gaattgaaaa aagcatccac gcgtaatgac tacttaggga tcaatcacta ccaatgccat
     5641 tttgtcaaag cttacgatgg agaaaatgca atccatcata acggtacggg tgaaaaaggg
     5701 acctctgttt ataaagtgaa gggaatcgga gaacgtattt ataaagaagg aattcctcga
     5761 actgattggg actggctgat ttatccagaa ggtctatatg acttgttgct gcgaattaaa
     5821 agcgattacc cacattacaa caaaatctat atcactgaga acgggatggg gtataaagat
     5881 cagtttgaag atggcattat tatggatcag ccgagaatcg actatttgag agtctatttg
     5941 gaatccttaa gcaaggcgat aacagccggg gtaaatgtga aggggtattt tttgtggtcg
     6001 cttatggatc ttttctcatg gacaaatggg tataacaaaa ggtatggctt gttttatgtt
     6061 gattttgaaa cccagaagcg ttacccaaaa gaatcagctt actggtacaa gttggtcagt
     6121 gaaaccaaaa cgattattta ataagtgata atggaggaag aaaagatgaa taaagaggaa
     6181 ttacaaatgc ttggttttga gatcgttgca tattcaggcg acgctcgcag tacattattg
     6241 aaattattga aagaagtaag acaaggaaac tttgaacatg ttgattcggc gttgaaagaa
     6301 gccgacgaaa atttaaccct tgcacataat tcgcaaacaa aaattttagc ggaagaagca
     6361 tctgggaaag agatggagat gggattcatt tttatccatg ggcaggatca tttaatgacc
     6421 actttattgc tgagagatct gattcaagac tttattgtac tgtacagaca gaatcaagga
     6481 taggagaaga acgctatgga tggaatcatt aaacaaatcg aaaaagggaa accatttttt
     6541 gaaaaaatct cacgtaatag ttatttagga gccgttcgtg acggattttt aacagcgatg
     6601 ccagctattt tattttcaag cattttcatt ttgattgctg cggttcctga gatttttggt
     6661 taccaatggc ctgaatctgt ctccacatgg ctatggaaag tctataatta ttcgatgggt
     6721 gttgtagggt tactcgtttc agcaaccact gctcgctgct tggctgattc gatgaatcgt
     6781 cgcttaccga aaaacaaagt aatcaacacc acctctgtga tgctggcctc tgtggtaagt
     6841 ttcctgttgt taagcgtctc tccgattgaa ggtggatttt caacggaata tatggggaca
     6901 aagggactac tggcttcctt tgtcgctgca tttttaacag ttaatttata taagttttgt
     6961 gtgctgaaag atattaccat taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc
     7021 ttccgagata tttttccttt ctctttttca gtatttgcgg ccgttttaat tgatttaggc
     7081 attcgacaaa tcttccaaat ctcatttgca gaagcaatca ttacggtgct gcagccgcta
     7141 tttacagcag ctgacgggta cttaggaatc gctattatct ggggagcgat ggcgctgttt
     7201 tggtttgtcg gtgttcatgg accgtcgatc gtggagccgg caattgcggc aattatctat
     7261 gccaatgtgg aaaccaatct gcaattgttc aattccggtg aacaagcttc taaagtactg
     7321 acggtgggtt tagggaattt tgtaggaact atgggaggaa ctggggcaac attggttgtt
     7381 ccttttatct tcctactctt tgctaagtct aagcagttga aggcagttgg gaaagcttcc
     7441 tttattccag taagtttcgc tgtcaacgaa ccattgctat ttgcggcacc catgattttg
     7501 aacccttatt tttttattcc attttttgtt aacacctgtt gtaaacgttt gcctgttcaa
     7561 gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc
     7621 agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc
     7681 ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt
     7741 cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc
     7801 cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga
     7861 aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt
     7921 aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc
     7981 tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta
     8041 ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg
     8101 agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca
     8161 atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat
     8221 ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg
     8281 aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat
     8341 attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact
     8401 atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag
     8461 ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta
     8521 aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc
     8581 ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa
     8641 agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca
     8701 atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag
     8761 aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac
     8821 ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt
     8881 ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt
     8941 atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac
     9001 ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg
     9061 atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac
     9121 cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata
     9181 atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg
     9241 caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca
     9301 accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag
     9361 caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca
     9421 gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg
     9481 aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg
     9541 catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg
     9601 taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg
     9661 aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc
     9721 caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga
     9781 cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat
     9841 cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc
     9901 ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc
     9961 agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat
    10021 gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta
    10081 gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac
    10141 agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata
    10201 gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa
    10261 gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt cccactccac
    10321 tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt
    10381 tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt
    10441 cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc
    10501 acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg
    10561 gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc
    10621 tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg
    10681 ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc
    10741 tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc
    10801 tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt
    10861 atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc
    10921 gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga
    10981 tacacctttc aacttgtcta agaagcgatc tcttgcattg actttgatga tgttcaagcc
    11041 gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg
    11101 gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac
    11161 actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg
    11221 gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca
    11281 aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc
    11341 atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc
    11401 tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct
    11461 catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac
    11521 ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt
    11581 gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat
    11641 ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat
    11701 gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc
    11761 ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta
    11821 gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac
    11881 ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc
    11941 gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc
    12001 ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt
    12061 ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg
    12121 gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca
    12181 gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct
    12241 gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga
    12301 tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta
    12361 tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc atatcataac
    12421 tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa
    12481 gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg
    12541 tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa
    12601 taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt
    12661 caaattcttt catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat
    12721 cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag
    12781 gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca
    12841 ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa
    12901 taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat ctttataata
    12961 gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt
    13021 catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga
    13081 tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt
    13141 aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga
    13201 tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta
    13261 tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat
    13321 taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt
    13381 tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt
    13441 aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata
    13501 tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc
    13561 gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa
    13621 aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga
    13681 aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt
    13741 tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt
    13801 tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa
    13861 aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt
    13921 ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag
    13981 atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag
    14041 gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt
    14101 ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga
    14161 tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt
    14221 tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga
    14281 tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa
    14341 atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt
    14401 taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg
    14461 gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga
    14521 aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga
    14581 aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag
    14641 gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg
    14701 ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc
    14761 cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc
    14821 aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga
    14881 tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa
    14941 ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt
    15001 aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt
    15061 ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat
    15121 gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat
    15181 cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt
    15241 attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc
    15301 gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa
    15361 gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag
    15421 tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact
    15481 agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg
    15541 cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga
    15601 caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc
    15661 cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac
    15721 cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat
    15781 gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga
    15841 acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca
    15901 aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt
    15961 tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga
    16021 atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc
    16081 gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa
    16141 acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct
    16201 aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca
    16261 taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga
    16321 cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc
    16381 aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac
    16441 cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt
    16501 gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca
    16561 tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg
    16621 tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac
    16681 aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat
    16741 caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt
    16801 tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt gttgaaacat cgttattaga
    16861 tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa
    16921 aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga
    16981 aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc
    17041 aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt
    17101 atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc
    17161 aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc
    17221 cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac
    17281 attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca
    17341 gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct
    17401 atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg
    17461 gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct
    17521 tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa
    17581 ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag
    17641 gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg
    17701 attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag
    17761 gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga
    17821 aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat
    17881 cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta
    17941 ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact
    18001 ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag
    18061 acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag
    18121 tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag
    18181 ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg
    18241 ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa
    18301 acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa
    18361 aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat
    18421 cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac
    18481 tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc
    18541 ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac
    18601 aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg
    18661 acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac
    18721 acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc
    18781 ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac
    18841 gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa
    18901 aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga
    18961 acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg
    19021 cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag
    19081 ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa
    19141 gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact
    19201 gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa
    19261 aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat
    19321 gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt
    19381 taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa
    19441 attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg
    19501 attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt
    19561 ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg
    19621 atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc
    19681 gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac
    19741 ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg
    19801 cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag
    19861 ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga
    19921 cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat
    19981 atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca
    20041 gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa
    20101 cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa
    20161 gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt
    20221 gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta
    20281 ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat
    20341 gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta
    20401 ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca
    20461 catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt
    20521 aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta
    20581 ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca
    20641 agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt
    20701 caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact
    20761 tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt
    20821 agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga
    20881 agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca
    20941 tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa
    21001 tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg
    21061 attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt
    21121 aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag
    21181 tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg
    21241 tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat
    21301 tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt
    21361 atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg
    21421 tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa
    21481 cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt
    21541 tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg
    21601 actatcgaat taattgcctt gatccttact tctacactgg tttattcaac accgctaatt
    21661 ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag
    21721 ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca
    21781 cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt
    21841 tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca
    21901 gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa
    21961 ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat
    22021 attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt
    22081 ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt
    22141 tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt
    22201 tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag
    22261 tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct
    22321 gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt
    22381 tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca
    22441 gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg
    22501 aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc
    22561 gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca
    22621 ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg
    22681 attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata
    22741 cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt
    22801 taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga
    22861 cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg
    22921 gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca
    22981 aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag
    23041 ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt
    23101 ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat
    23161 atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa
    23221 tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg
    23281 attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg
    23341 acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg
    23401 tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt
    23461 tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg
    23521 atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg
    23581 aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt
    23641 ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga
    23701 aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga
    23761 actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt
    23821 tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt
    23881 tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg
    23941 tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt
    24001 tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa
    24061 gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt
    24121 gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc
    24181 atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat
    24241 caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt
    24301 tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga
    24361 aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga
    24421 aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac
    24481 tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat
    24541 taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac
    24601 tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata
    24661 ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga
    24721 ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc
    24781 cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac
    24841 gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat
    24901 tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa
    24961 aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga
    25021 tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga
    25081 aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg
    25141 aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt
    25201 taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga
    25261 cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag
    25321 aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat
    25381 aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc
    25441 tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt
    25501 taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac
    25561 agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg
    25621 tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt
    25681 tataggtagt attttttagc aactgttaag tcatcattta attcataaac aagtggttga
    25741 cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct
    25801 agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct
    25861 ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct
    25921 ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct
    25981 tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact
    26041 tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag
    26101 tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct
    26161 aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat
    26221 tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt
    26281 gctagattta catcagccca accagtaaat tggttcaatg cattccattc actaagaccg
    26341 tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc
    26401 tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt
    26461 agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa
    26521 ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata
    26581 tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct
    26641 tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt
    26701 ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc
    26761 aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg
    26821 cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc
    26881 gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt
    26941 gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt
    27001 tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca
    27061 acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg
    27121 gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag
    27181 tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa
    27241 gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg
    27301 acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca
    27361 tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg
    27421 taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg
    27481 aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact
    27541 gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt
    27601 tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc
    27661 ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact
    27721 tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa
    27781 gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc
    27841 atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc
    27901 gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt
    27961 gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt
    28021 ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc
    28081 gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt
    28141 tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga
    28201 accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta
    28261 caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg
    28321 ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa
    28381 aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac
    28441 gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga
    28501 ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact
    28561 taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc
    28621 gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa
    28681 acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga
    28741 ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat
    28801 ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg
    28861 gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga
    28921 tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc
    28981 acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt
    29041 tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga
    29101 agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa
    29161 acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta
    29221 agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag
    29281 caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa
    29341 cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg
    29401 tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca
    29461 aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac
    29521 agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac
    29581 agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca
    29641 atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc
    29701 tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc
    29761 aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga
    29821 agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga
    29881 tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga
    29941 atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata
    30001 cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt
    30061 taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt
    30121 actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg
    30181 gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc
    30241 agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt
    30301 gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga
    30361 acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta
    30421 cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg
    30481 tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt
    30541 cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact
    30601 atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat
    30661 cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa
    30721 agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac
    30781 tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg
    30841 gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg
    30901 tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga
    30961 aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc
    31021 ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg
    31081 tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt
    31141 tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt
    31201 tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc
    31261 tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac
    31321 aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa
    31381 aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag agattatcca
    31441 aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg
    31501 gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg
    31561 aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg
    31621 tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact
    31681 atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga
    31741 acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca
    31801 ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc
    31861 tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac
    31921 tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa
    31981 caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga
    32041 cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt
    32101 gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt
    32161 tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga
    32221 agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca
    32281 agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac
    32341 agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat
    32401 tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa
    32461 accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga
    32521 agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac
    32581 aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga
    32641 caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt
    32701 ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta
    32761 attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg
    32821 tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag
    32881 tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg
    32941 aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg
    33001 acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta
    33061 agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt
    33121 tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa
    33181 aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa
    33241 tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg
    33301 aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat
    33361 tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg
    33421 atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact
    33481 aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa
    33541 tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc
    33601 caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg
    33661 gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag
    33721 caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg
    33781 aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag
    33841 gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac
    33901 caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta
    33961 accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc
    34021 aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg
    34081 ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt
    34141 aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc
    34201 aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg
    34261 tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa
    34321 accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg
    34381 cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa
    34441 caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg
    34501 tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg
    34561 caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat
    34621 tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg
    34681 tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt
    34741 tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga
    34801 cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata
    34861 cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc
    34921 ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat
    34981 gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga
    35041 ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa
    35101 ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac
    35161 atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa
    35221 ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt
    35281 ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt
    35341 gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac
    35401 tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa
    35461 cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt
    35521 ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc
    35581 cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc
    35641 ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta
    35701 ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt
    35761 aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt
    35821 gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt
    35881 ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa
    35941 aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt
    36001 agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca
    36061 ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt
    36121 tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa
    36181 gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat
    36241 gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga
    36301 acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg
    36361 tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt
    36421 cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc
    36481 agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga
    36541 aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa
    36601 acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc
    36661 aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt
    36721 atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt
    36781 caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg
    36841 taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag
    36901 tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg
    36961 tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat
    37021 tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg
    37081 ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc
    37141 gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt
    37201 acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc
    37261 gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt
    37321 acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg
    37381 cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac
    37441 aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa
    37501 agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat
    37561 gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat
    37621 gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca
    37681 caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga
    37741 aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa
    37801 aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac
    37861 gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta
    37921 agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt
    37981 atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa
    38041 agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg
    38101 atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat
    38161 cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac
    38221 acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc
    38281 aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca
    38341 aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg
    38401 ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg
    38461 ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg
    38521 ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg
    38581 cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat
    38641 aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata
    38701 aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc
    38761 cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt
    38821 ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg
    38881 tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca
    38941 aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag
    39001 gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat
    39061 tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca
    39121 tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag
    39181 aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg
    39241 gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa
    39301 tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg
    39361 gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat
    39421 tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta
    39481 ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc
    39541 cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa
    39601 acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc
    39661 cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata
    39721 aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg
    39781 aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa
    39841 cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat
    39901 cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa
    39961 acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa
    40021 cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa
    40081 agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa
    40141 agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag
    40201 aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg
    40261 aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat
    40321 tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca
    40381 ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca
    40441 acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg
    40501 ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag
    40561 tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg
    40621 ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc
    40681 ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag
    40741 aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa
    40801 ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat
    40861 ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt
    40921 gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac
    40981 aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc
    41041 tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat
    41101 aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct
    41161 gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac
    41221 catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag
    41281 aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg
    41341 ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg
    41401 ctttagttgt tgtcggtgac aaaaacggac acgtagggtt tggtacaggt aaagctcaag
    41461 aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac
    41521 ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc
    41581 taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat
    41641 tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca
    41701 acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag
    41761 aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga
    41821 attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt
    41881 taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat
    41941 taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa
    42001 aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg
    42061 ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa
    42121 aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag
    42181 gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg
    42241 tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag
    42301 ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa
    42361 tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct
    42421 ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt
    42481 tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt
    42541 atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc
    42601 ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag
    42661 tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat
    42721 cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg
    42781 ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt
    42841 tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa
    42901 taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt
    42961 tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat
    43021 catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta
    43081 ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact
    43141 agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat
    43201 cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa
    43261 agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa
    43321 tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat
    43381 ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc
    43441 cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca
    43501 aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc
    43561 aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct
    43621 tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag
    43681 cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct
    43741 gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac
    43801 acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag
    43861 gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct
    43921 caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa
    43981 agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag
    44041 agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc
    44101 tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg
    44161 tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct
    44221 gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat
    44281 gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta
    44341 aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac
    44401 aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac
    44461 aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct
    44521 tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga
    44581 tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa
    44641 atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa
    44701 ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac
    44761 caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt
    44821 gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc
    44881 tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag
    44941 taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt
    45001 gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc
    45061 tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat
    45121 ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga
    45181 tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa
    45241 attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga
    45301 aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac
    45361 gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata
    45421 attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg
    45481 tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca ataacactat
    45541 catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg
    45601 tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac
    45661 aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc
    45721 tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg
    45781 tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg
    45841 agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga
    45901 aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag
    45961 aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag
    46021 ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc
    46081 aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc
    46141 aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc
    46201 ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg
    46261 atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc
    46321 atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag
    46381 aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga
    46441 actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg
    46501 agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca
    46561 tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca
    46621 tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag
    46681 acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac
    46741 ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag
    46801 aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc
    46861 ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac
    46921 gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta
    46981 ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac
    47041 gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg
    47101 tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata
    47161 ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa
    47221 gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca
    47281 tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta
    47341 gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt
    47401 tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt
    47461 aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat
    47521 gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta taaataccag
    47581 ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg
    47641 atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga
    47701 ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat
    47761 atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa tcagtttgtt
    47821 ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa
    47881 gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca
    47941 cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt
    48001 gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt
    48061 cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata
    48121 tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa
    48181 ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc
    48241 gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg
    48301 gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc
    48361 gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat
    48421 ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa
    48481 gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag
    48541 tccatattgg agaacgagat attcagccgg atacggataa taagaattta aagcccatcc
    48601 gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag
    48661 caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc
    48721 tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat
    48781 ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc
    48841 cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggt agaaaagaaa
    48901 tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc
    48961 atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa
    49021 tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac
    49081 aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact
    49141 ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag
    49201 gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc
    49261 catcgattgg atccccgagc taaattatta gccagttttt attttatcgg aatcattttt
    49321 ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc
    49381 ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc
    49441 ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg
    49501 ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc
    49561 gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg
    49621 gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt
    49681 tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag
    49741 atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa
    49801 atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa
    49861 gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat
    49921 cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt attattaact
    49981 gtgctactga tctttttgag aggataaacg ttcagtgggc cgctatctat tcttttcttg
    50041 ctgatctgtt tgcaagaaag gatgcgagca gtgtatgaca agaaatatta gggtctgtgg
    50101 cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata
    50161 aaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta
    50221 caaagcaatc attgcatacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg
    50281 gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat
    50341 cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgattgggc aagtaatcca
    50401 ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc
    50461 accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata
    50521 tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt
    50581 tcgacgccat tatgcaagtt attttcctta ccctcttgat ttggaaaaaa ttcagcgggc
    50641 gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat
    50701 agaggataaa gtacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga
    50761 attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg
    50821 aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc
    50881 gaaaaaagat cgtaatgcag caggacctac agcacatcct gaaggacttt atttgtatga
    50941 agtgaaatat gaataagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat
    51001 ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa ggagctactt
    51061 ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
    51121 ccaaacaaaa atagctcgtc atatttttcg tttggttggg cttattaccg cagatgcaag
    51181 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagag
    51241 aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgaaggtca
    51301 gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
    51361 gtaacaattt caaaaaacag cttctcatct ttgaggaata tcgtgacttg tggacgaggc
    51421 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
    51481 gttgttttga ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt tat
//
LOCUS       DAFFQY010000009        49878 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFFQY010000009 DAFFQY010000000
VERSION     DAFFQY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.009"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(76..273)
                     /locus_tag="LRB02_000459"
     CDS             complement(76..273)
                     /locus_tag="LRB02_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL4658694.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(667..2394)
                     /locus_tag="LRB02_000460"
     CDS             complement(667..2394)
                     /locus_tag="LRB02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL4658695.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2387..3580)
                     /locus_tag="LRB02_000461"
     CDS             complement(2387..3580)
                     /locus_tag="LRB02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4658696.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3767..4411)
                     /locus_tag="LRB02_000462"
     CDS             complement(3767..4411)
                     /locus_tag="LRB02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4658697.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4505..4639)
                     /locus_tag="LRB02_000463"
     CDS             complement(4505..4639)
                     /locus_tag="LRB02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL4658698.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4641..6772)
                     /gene="feoB"
                     /locus_tag="LRB02_000464"
                     /pseudo
     CDS             complement(4641..6772)
                     /gene="feoB"
                     /locus_tag="LRB02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
     gene            complement(6769..7242)
                     /locus_tag="LRB02_000465"
     CDS             complement(6769..7242)
                     /locus_tag="LRB02_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL4658699.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7643..7867
                     /gene="nrdH"
                     /locus_tag="LRB02_000466"
     CDS             7643..7867
                     /gene="nrdH"
                     /locus_tag="LRB02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL4658700.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8026..10185
                     /gene="nrdE"
                     /locus_tag="LRB02_000467"
     CDS             8026..10185
                     /gene="nrdE"
                     /locus_tag="LRB02_000467"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL4658701.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10235..11200
                     /gene="nrdF"
                     /locus_tag="LRB02_000468"
     CDS             10235..11200
                     /gene="nrdF"
                     /locus_tag="LRB02_000468"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL4658702.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11289..12428)
                     /locus_tag="LRB02_000469"
     CDS             complement(11289..12428)
                     /locus_tag="LRB02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4658703.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12737..13450)
                     /locus_tag="LRB02_000470"
     CDS             complement(12737..13450)
                     /locus_tag="LRB02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL4658704.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13704..14405)
                     /gene="nagB"
                     /locus_tag="LRB02_000471"
     CDS             complement(13704..14405)
                     /gene="nagB"
                     /locus_tag="LRB02_000471"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL4658705.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14639..16171
                     /locus_tag="LRB02_000472"
     CDS             14639..16171
                     /locus_tag="LRB02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4658706.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16362..17564)
                     /locus_tag="LRB02_000473"
     CDS             complement(16362..17564)
                     /locus_tag="LRB02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4658707.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17646..18335)
                     /locus_tag="LRB02_000474"
     CDS             complement(17646..18335)
                     /locus_tag="LRB02_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4658708.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18441..18809)
                     /gene="rplL"
                     /locus_tag="LRB02_000475"
     CDS             complement(18441..18809)
                     /gene="rplL"
                     /locus_tag="LRB02_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL4658709.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18862..19365)
                     /locus_tag="LRB02_000476"
     CDS             complement(18862..19365)
                     /locus_tag="LRB02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL4658710.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19569..19696)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19775..20464)
                     /gene="rplA"
                     /locus_tag="LRB02_000477"
     CDS             complement(19775..20464)
                     /gene="rplA"
                     /locus_tag="LRB02_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL4658711.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20568..20990)
                     /gene="rplK"
                     /locus_tag="LRB02_000478"
     CDS             complement(20568..20990)
                     /gene="rplK"
                     /locus_tag="LRB02_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL4658712.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21333..22001
                     /gene="sdaAB"
                     /locus_tag="LRB02_000479"
     CDS             21333..22001
                     /gene="sdaAB"
                     /locus_tag="LRB02_000479"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL4658713.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22014..22886
                     /gene="sdaAA"
                     /locus_tag="LRB02_000480"
     CDS             22014..22886
                     /gene="sdaAA"
                     /locus_tag="LRB02_000480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL4658714.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22977..23996)
                     /locus_tag="LRB02_000481"
     CDS             complement(22977..23996)
                     /locus_tag="LRB02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL4658715.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24169..25074)
                     /locus_tag="LRB02_000482"
     CDS             complement(24169..25074)
                     /locus_tag="LRB02_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4658716.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25133..25678)
                     /gene="nusG"
                     /locus_tag="LRB02_000483"
     CDS             complement(25133..25678)
                     /gene="nusG"
                     /locus_tag="LRB02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL4658717.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25779..25949)
                     /gene="secE"
                     /locus_tag="LRB02_000484"
     CDS             complement(25779..25949)
                     /gene="secE"
                     /locus_tag="LRB02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL4658718.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25973..26125)
                     /gene="rpmG"
                     /locus_tag="LRB02_000485"
     CDS             complement(25973..26125)
                     /gene="rpmG"
                     /locus_tag="LRB02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4658719.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26287..26931
                     /locus_tag="LRB02_000486"
     CDS             26287..26931
                     /locus_tag="LRB02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4658720.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26987..27490)
                     /locus_tag="LRB02_000487"
     CDS             complement(26987..27490)
                     /locus_tag="LRB02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL4658721.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27503..27549)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28087..29052
                     /locus_tag="LRB02_000488"
     CDS             28087..29052
                     /locus_tag="LRB02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL4658722.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29151..30323)
                     /locus_tag="LRB02_000489"
     CDS             complement(29151..30323)
                     /locus_tag="LRB02_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658723.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30310..31455)
                     /locus_tag="LRB02_000490"
     CDS             complement(30310..31455)
                     /locus_tag="LRB02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658724.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31469..32393)
                     /locus_tag="LRB02_000491"
                     /pseudo
     CDS             complement(31469..32393)
                     /locus_tag="LRB02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32510..33589
                     /locus_tag="LRB02_000492"
     CDS             32510..33589
                     /locus_tag="LRB02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4658725.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33621..34214
                     /locus_tag="LRB02_000493"
     CDS             33621..34214
                     /locus_tag="LRB02_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4658726.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34324..34551)
                     /locus_tag="LRB02_000494"
     CDS             complement(34324..34551)
                     /locus_tag="LRB02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4658727.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34654..37242)
                     /locus_tag="LRB02_000495"
     CDS             complement(34654..37242)
                     /locus_tag="LRB02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL4658728.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37262..37921)
                     /locus_tag="LRB02_000496"
     CDS             complement(37262..37921)
                     /locus_tag="LRB02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4658729.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37970..38473)
                     /gene="trmL"
                     /locus_tag="LRB02_000497"
     CDS             complement(37970..38473)
                     /gene="trmL"
                     /locus_tag="LRB02_000497"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL4658730.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38694..39560)
                     /locus_tag="LRB02_000498"
     CDS             complement(38694..39560)
                     /locus_tag="LRB02_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4658731.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39669..40310
                     /locus_tag="LRB02_000499"
     CDS             39669..40310
                     /locus_tag="LRB02_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL4658732.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40372..41733)
                     /gene="mgtE"
                     /locus_tag="LRB02_000500"
     CDS             complement(40372..41733)
                     /gene="mgtE"
                     /locus_tag="LRB02_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL4658733.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41751..42650)
                     /locus_tag="LRB02_000501"
     CDS             complement(41751..42650)
                     /locus_tag="LRB02_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4658734.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42652..43449)
                     /locus_tag="LRB02_000502"
     CDS             complement(42652..43449)
                     /locus_tag="LRB02_000502"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL4658735.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43427..44131)
                     /locus_tag="LRB02_000503"
     CDS             complement(43427..44131)
                     /locus_tag="LRB02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL4658736.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44262..44837
                     /locus_tag="LRB02_000504"
     CDS             44262..44837
                     /locus_tag="LRB02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL4658737.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44955..45632
                     /locus_tag="LRB02_000505"
     CDS             44955..45632
                     /locus_tag="LRB02_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL4658738.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45711..47519)
                     /gene="pepF"
                     /locus_tag="LRB02_000506"
     CDS             complement(45711..47519)
                     /gene="pepF"
                     /locus_tag="LRB02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL4658739.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47695..48693)
                     /locus_tag="LRB02_000507"
     CDS             complement(47695..48693)
                     /locus_tag="LRB02_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL4658740.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48919..49572)
                     /locus_tag="LRB02_000508"
     CDS             complement(48919..49572)
                     /locus_tag="LRB02_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL4658741.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14930 a        10470 c         8761 g        15717 t
ORIGIN      
        1 agaatattta ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt
       61 agcatttttt tcattttagc acttccctgg aattgctcca cctaaaaaac cacctataga
      121 cattcctgca atacaagtag ttgccttggc ccaatcgacc gtacatttat ttttagtgca
      181 atacactcca tttccataat attttccact atgagtggta cccccataga taagttgtgt
      241 ctctttaata gacaaaattt ttaaatgttt cataatctaa tcacccctaa attaatgttt
      301 ttagactatt attctaacat tcattaaatt tcaaaaaaca attccttctt gaaacgtagc
      361 aaaataattc ctgaataaga atttaatcac ttttaagtat ataaactatg tatattcttt
      421 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
      481 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg
      541 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      601 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      661 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      721 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      781 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      841 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
      901 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
      961 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
     1021 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
     1081 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
     1141 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
     1201 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
     1261 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
     1321 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
     1381 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1441 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1501 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1561 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1621 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1681 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1741 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1801 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1861 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     1921 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     1981 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     2041 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     2101 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     2161 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     2221 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     2281 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     2341 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     2401 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2461 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2521 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2581 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2641 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2701 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2761 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2821 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     2881 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     2941 gaacattagc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     3001 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     3061 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     3121 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     3181 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     3241 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     3301 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     3361 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3421 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3481 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3541 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3601 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3661 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3721 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3781 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3841 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     3901 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     3961 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     4021 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     4081 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     4141 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     4201 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     4261 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     4321 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     4381 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4441 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4501 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4561 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4621 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4681 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4741 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4801 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4861 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     4921 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     4981 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     5041 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     5101 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     5161 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     5221 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     5281 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     5341 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     5401 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5461 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5521 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5581 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5641 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5701 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5761 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5821 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     5881 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     5941 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     6001 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     6061 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     6121 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     6181 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     6241 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     6301 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     6361 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt ccttcttttt
     6421 tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg
     6481 tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag
     6541 gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag
     6601 aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt
     6661 ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat
     6721 tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc
     6781 ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg
     6841 cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt
     6901 tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac
     6961 gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc
     7021 agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact
     7081 ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc
     7141 aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac
     7201 ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg
     7261 aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa
     7321 ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt
     7381 gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc
     7441 atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt
     7501 cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta
     7561 gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag ataataagaa
     7621 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
     7681 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
     7741 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
     7801 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
     7861 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
     7921 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
     7981 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
     8041 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
     8101 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
     8161 aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
     8221 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
     8281 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
     8341 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
     8401 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
     8461 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
     8521 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
     8581 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     8641 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     8701 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     8761 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     8821 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     8881 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     8941 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     9001 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     9061 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     9121 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     9181 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaacg
     9241 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     9301 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     9361 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     9421 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     9481 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     9541 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     9601 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     9661 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     9721 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     9781 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     9841 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     9901 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaatggca
     9961 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
    10021 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
    10081 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
    10141 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
    10201 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
    10261 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
    10321 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
    10381 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
    10441 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
    10501 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
    10561 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
    10621 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
    10681 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
    10741 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
    10801 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
    10861 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
    10921 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
    10981 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
    11041 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
    11101 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
    11161 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
    11221 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
    11281 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
    11341 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
    11401 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
    11461 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
    11521 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
    11581 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
    11641 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
    11701 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
    11761 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
    11821 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
    11881 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
    11941 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
    12001 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
    12061 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
    12121 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
    12181 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
    12241 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaact
    12301 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
    12361 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
    12421 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
    12481 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
    12541 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
    12601 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
    12661 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
    12721 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
    12781 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
    12841 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
    12901 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
    12961 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
    13021 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
    13081 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
    13141 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
    13201 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
    13261 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
    13321 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
    13381 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
    13441 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
    13501 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
    13561 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
    13621 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
    13681 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
    13741 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
    13801 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
    13861 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
    13921 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
    13981 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
    14041 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
    14101 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
    14161 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
    14221 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
    14281 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
    14341 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
    14401 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
    14461 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
    14521 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
    14581 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
    14641 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
    14701 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
    14761 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcaa
    14821 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
    14881 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
    14941 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
    15001 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
    15061 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
    15121 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
    15181 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
    15241 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
    15301 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
    15361 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
    15421 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
    15481 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
    15541 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
    15601 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
    15661 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
    15721 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
    15781 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
    15841 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
    15901 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
    15961 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
    16021 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
    16081 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
    16141 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
    16201 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
    16261 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
    16321 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
    16381 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
    16441 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
    16501 ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg gttgcttgct
    16561 gcttcaccta gcccttgtac tcgtgtttga ttggcgactt gaccaaattg attcccgacg
    16621 tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac caataaagca
    16681 ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc agaataagaa
    16741 tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa aatcgctaat
    16801 atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa aatcaacgta
    16861 gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc ttctcttaat
    16921 agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta tgaaacagct
    16981 gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt aagaattccg
    17041 actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc aacaatacca
    17101 gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt acccatgacc
    17161 ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg
    17221 atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa
    17281 aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa ttttggacga
    17341 tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc taattgggtc
    17401 aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc tacttttgtt
    17461 ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat tgataatagt
    17521 aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt cccccatttc
    17581 ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac tattcgccac
    17641 acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata aatgaagaca
    17701 actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt gttatatccg
    17761 tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac tccaatcacg
    17821 atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa attgaccata
    17881 gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg atgtttcatt
    17941 tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa ttttaagcga
    18001 tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt gatgatcgca
    18061 cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt aggaatacct
    18121 ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc
    18181 gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa taattggatg
    18241 tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat gattcccaac
    18301 gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa aaatctatat
    18361 tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca taggatctca
    18421 gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta
    18481 gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca
    18541 tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata
    18601 actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca
    18661 gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat
    18721 tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca
    18781 gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt
    18841 atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt
    18901 gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag
    18961 tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc
    19021 ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat
    19081 tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac
    19141 ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat
    19201 aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac
    19261 tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac
    19321 ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct
    19381 cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt
    19441 tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct actcgctttg
    19501 ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg
    19561 ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat
    19621 atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta
    19681 caagttaatt attaacctca actagcttag catgctaaac tagttgagtc aagaattatt
    19741 tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga
    19801 taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg
    19861 atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat
    19921 cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag
    19981 ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag
    20041 ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag
    20101 ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt
    20161 gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt
    20221 ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag
    20281 caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa
    20341 ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag
    20401 gataaacttt acttgaatca acttttttta atgcatcttg catttttttg ctctttttag
    20461 ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc
    20521 tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg
    20581 atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg
    20641 tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca
    20701 actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga
    20761 acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata
    20821 acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat
    20881 cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct
    20941 ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc
    21001 tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg
    21061 atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa
    21121 tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta
    21181 aatttattct aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc
    21241 gctagcatgg ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa
    21301 agattcctac ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct
    21361 ttgatattat tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta
    21421 gaatcgggaa aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc
    21481 tatatgaatc atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg
    21541 gcctgttagg gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg
    21601 aagccggtat ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg
    21661 taaagatgct tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg
    21721 gtggcaatat ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc
    21781 ctaccttgat cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac
    21841 tttctgagac caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa
    21901 atgccattat gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca
    21961 aacagctgcc aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt
    22021 tatcgattga agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga
    22081 ttgcagttga aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa
    22141 aaaacctctc agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca
    22201 caggaattac cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt
    22261 taagtggaga aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg
    22321 caaaaatggg gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg
    22381 tgctgatggc tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat
    22441 ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag
    22501 ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg
    22561 caaaaaatgg gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga
    22621 tgggactgat ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg
    22681 ctttaggctc ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg
    22741 tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga
    22801 ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg
    22861 aagagatttt aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag
    22921 ttatcttcat aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa
    22981 tccatcaatt tcgtattttt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg
    23041 atcgacaatt ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga
    23101 ttcaacccat aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct
    23161 ttgaagacat gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg
    23221 actacttcgg caatttttgg caatacttca aaggacgcag gacctccatc tagttgacgt
    23281 ccaccatgat tggataccca gataccatct gcccctgcag agatagcgat tactgcatct
    23341 tctggcgatt gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg
    23401 atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct
    23461 tcgccattac cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga
    23521 aattggaatt gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt
    23581 aaaatgatag ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg
    23641 tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg
    23701 tctttgattg tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact
    23761 cctttagctg tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct
    23821 tggataatcg gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg
    23881 attccgcgta atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc
    23941 atcgtccact catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc
    24001 tcttttacac gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta
    24061 cttgtatgat aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac
    24121 taaacttaca aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca
    24181 aataaatcga cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc
    24241 attttcacct gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata
    24301 gaccgctaat gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg
    24361 accagcaatc aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc
    24421 ttgatagcgg ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc
    24481 actatcatcg acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg
    24541 caatgacgta tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga
    24601 atgccccact atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt
    24661 tttgatccat tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac
    24721 catcggattg tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc
    24781 tactgttaaa accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc
    24841 tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt
    24901 ttctatcact ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat
    24961 cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt
    25021 tgcacgtttg cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa
    25081 aaaagaaaga cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga
    25141 ttttatctac ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt
    25201 tgactttcaa tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa
    25261 atgcgccttc gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca
    25321 ggctcatacc gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt
    25381 tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat
    25441 accatgaagc gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa
    25501 cgatttcttt ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac
    25561 ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt
    25621 tgttttcata tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg
    25681 aaggctcctc atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg
    25741 cttcattata gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat
    25801 aagctttgga tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg
    25861 atgactacta acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt
    25921 tcatctttta cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg
    25981 tttctttatg taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt
    26041 cgccgctttt tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag
    26101 cgagtgctgc ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata
    26161 cttcataaca gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt
    26221 tttggataaa attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt
    26281 atagcgatgc tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag
    26341 actagtacat tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg
    26401 atcttagatt cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt
    26461 cataaagcaa atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca
    26521 aagttcattt acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct
    26581 tatattgctg tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg
    26641 cttaatatgc tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct
    26701 tccacaggaa agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc
    26761 attgccagct gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc
    26821 acgctaccgg ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa
    26881 aagaattata aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc
    26941 gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt
    27001 caaaatcaag cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa
    27061 actcacttaa tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta
    27121 acatataagc aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca
    27181 tctttacatt tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt
    27241 ggccgccacc aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga
    27301 ccccgcctaa aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt
    27361 ctgaaatacg aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta
    27421 gataaagagc catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg
    27481 ttttttgcat tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac
    27541 cgcggttatg tgaaacaaac aataattata acagactttt catttatttc aatccgtctt
    27601 atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat
    27661 atggtataaa gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat
    27721 ctgcaagaac agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc
    27781 aacctcttac tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc
    27841 ttggaacttc tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt
    27901 cggtcttcgg acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt
    27961 gtaacagatt tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa aagcttgcta
    28021 attattggtt aaccagcaaa caggagtatt aaaatatctt gttttattga atagcaggag
    28081 gaaatcatga aattccatta tcatatattg atcaatccat cggcaggcag cggaaatggc
    28141 tataaagtag cagaaagaat tttaccggta ttaaaaaata agcatattga ctatacactt
    28201 cattatagtg aatacaaagg acacgatgct gagattgctg aaactttggc aaaagaaaca
    28261 ttgattcctt ggatagaaga agaacagaaa acaatcgata ctttccccct tttgatcatc
    28321 gtaggaggtg acggcacctt acatcaggta cttgatactt tctatcagat ggaagtagaa
    28381 ttcccagttg cttatattcc agcaggttct ggagatgatt ttgctcgagg ggcaggcctt
    28441 ccaaaaaatc ccaaaaaagg acttcagctg attttagcag ctcaatcacc agaaaaagtc
    28501 cacataatgg cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt taataatttc
    28561 ggtataggat tagatgcagc tatcgtccat gcgacaaatc attcttccac aaaaaagcga
    28621 ttgaacaaat acaacttagg atcattttcg tatttattct ctattctccg tgctttattc
    28681 actcaaaaag gatttccaat tctggtagat gcaggaggaa aacaattaag tttttcaaac
    28741 gcatttttat gtacagcaac gaagcatcct ttttttggcg gaggaatcgc gattgttcca
    28801 actgctgatg cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat caatttcttt
    28861 aaaatcttgt ggctcattct tttacttata cagaaaaaac aattgaaatc gaaatatttc
    28921 catcactaca caacaaacag attacgtatc gtttcaacaa caccgcaaca tggtcaagaa
    28981 gatggagaag aaatggaaaa acgacctttt gatatcacaa taaccacaac taaacagttg
    29041 ttttggtgtt aataaaagag gttttgtcag aagctacatc tggaacaccg tttattcgga
    29101 aaaaaggact gtgacacgac ttttgtcaca gtcctttttt tactatgcag ttaattcttt
    29161 gcccaattgg gttgccatcg atagtttctt tctttaccca aaataaattg gatcattgaa
    29221 aacaccgcag caaaagcaac taagacacaa ccattgaacc aaaaagattc tgcaaatgtt
    29281 tgattgtaac tgagtgtttc actgatcaag ttattatttt gaacaaacca atatgttggt
    29341 gtgaatgccg ctattttgct aaccatttct ggcaagtatt ctgctggtac aaacacccca
    29401 ccgataaagc aactgcctgt gataaaaata tctttgatcc caccgctggt ttctgagttt
    29461 tttaccaatg aagtgataca agccgaaaag ctaatgatcg gcaataaaaa caagaaagta
    29521 tttaaaatat gataccatac cgttatatcc cacgaggtgt ggatcactaa cagaaccatt
    29581 agaagtaaga agccactgaa caacagcagc gagtaactaa tcaaactaaa ggttactttg
    29641 cgattcaagt ttcgtttggt aatgggcgca ctctggtttc gtttactgat ctctttgcga
    29701 ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa cccataagac
    29761 aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc gctgactata tcccgcactt
    29821 agctcaactt ctccagtaac cgagagtgcc atttgtgtct gttccatagc tgcatcgatt
    29881 gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta
    29941 acaactgttt tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg catctttcct
    30001 acttcgactt gttcggtaaa gtctgcaggc agatgtaaga taatgtttac acgattaaaa
    30061 taaagagcat catcgatttc tttttgtgag gttccatcca atgtaacgac tgtttccttg
    30121 tcattcaaat atttacgaag gccatcgact acaggcgact cttcttcact aatcaaggcg
    30181 attttcgcat cttgcagaga ggtattttca acatcaatat tttgcgcaaa aaagaatgtg
    30241 gcaacaacag tgataattac acctatcagt aaattcgatt tatttgcttt aagcaatgtc
    30301 caaatcgttt tataaagcgt cataacgcac ccctctttcc atccaagcat ttcctaacgc
    30361 aaaaaggaaa ataaatccga tcaaccatag aagattggta taaaaactag ccaaatcagt
    30421 gtagaaataa agtttataca aactttcgct gatcaagttg acgagattta attgaccaac
    30481 aatcggaata tgaagatcaa tccaatactt cacttgttcc gaccccatca taccagcaaa
    30541 aaagctcatc gcaatcgtca ctgaaatcaa gatgctatcc ttttgcacca gattcatttt
    30601 tggtatcaaa ttaccaacca atgtacctaa caaaatcgca cacaacgacc ccagagcggt
    30661 aacaatcatc aaccattgcc aacgttcacc aaagtctact tgatagataa aacgaaagac
    30721 agctaacata atataaatct ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat
    30781 tgaaactaac gattgctttc taggaatcaa ggacaagcga ataccatttg acgattgatt
    30841 tgcttgctga tcctgtacat tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc
    30901 aatcagagtg aagaaataaa aacttttgaa actcatatta cgacctgcat gaacttcttg
    30961 aaaagatact tcttgttcaa aaacagtcat gatgggcgcg agatcaacgt tctccacttc
    31021 tgccacttta ctcgaaatta taccaatacg ctgtaaaaat tgatccatga cgtttttcat
    31081 caacgtctga ctcattcctg cttgacccac atggagcgaa acagatgaac catcaatgag
    31141 ataaaaacca gccaaatcat ctgcatttag cttttcattc gcttctactt ccgatagttc
    31201 ctgtacttca aataatggtt tatcattgac ttcgacttgt tctaagactg tggtaaaatt
    31261 ttccgaaagc gctgtttgat ccgctttgat tacaattcca gtattaatcg tttcgagatt
    31321 gccaccttga tcaatctcgc caaaggccgc cataaacatc aatcctaaaa gaattggaaa
    31381 ggccaatgtc caaaacaaca tggatttatt tcgcagcagt atttttgtgc gatatttata
    31441 aaaatgccaa aacattattt cccctcctct agtctctcaa atccttgcca gtaatctcta
    31501 aaaagacatc attcaaggac gcttctttgg ttgctatttt tccgtaactg atcttttctt
    31561 cttgtaagat tgtaagaatc ggcaacaata ctgtatttag ttcagtagtt gttacttgga
    31621 gctgaccttt tttataagcg attccatcag aagctattgt tcgctgacaa cacgttttct
    31681 gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc ttttagagat
    31741 tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg gtcgcaaaga
    31801 atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg tcgattcagc
    31861 tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac tgtcggctca
    31921 tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa ccgacgtttc
    31981 aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc cactaattga
    32041 atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa accgcaaaaa
    32101 taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg aggaacgacc
    32161 ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa caatttaatg
    32221 gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga tttcccactg
    32281 ccgtttggtc ctaacagacc taagacttct ccttcttgta cttgcagatc aaaatgattc
    32341 aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat catcatgaaa
    32401 aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa gaaactagtg
    32461 acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta tgcataaatc
    32521 tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc tggtaagtca
    32581 aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca ctggtgtcag
    32641 ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc ttttatgtgc
    32701 ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga
    32761 aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg ttggtaatca
    32821 tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa
    32881 aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag atactcattg
    32941 ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac aagaaacaca
    33001 attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc atgacaatgt
    33061 cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga tcaatcaaga
    33121 accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg ggatggatcg
    33181 gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac actccttaga
    33241 gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt
    33301 gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat
    33361 caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg ccttttatcg
    33421 cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg gtattggttt
    33481 gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt tccaagaaaa
    33541 ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat aacagtagtc
    33601 attattgaga tgatccattt gtgacgaaat cgttacaaac cattttagaa agcacgaaag
    33661 agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc
    33721 atcgtcccga tgttttgctg acggacatcc gcatgcctaa acaaacgggc atcgatgctg
    33781 ccaaagaaat tttggctaaa tttcccaaag ccatcctttt gcttttgacg acattcaaag
    33841 atgaagaata catccgcgaa gcattttcca tcggtgtcaa agggtattta atcaaacaga
    33901 acttgcaagc gattattcct tctgtcaaat ccgcttataa tggacaggcc gtatttggta
    33961 atgaaatcat cgagactttc actcaaatga tgaagaataa accgacgatt gatagtcatg
    34021 cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa agaagtagct gccgggaaaa
    34081 acaataaaga aattgcagat gccttgtact tgagtgacgg cactgttcgc aattacatca
    34141 gccaattact agaaaagcta gatttacggg atcgcacgca attggctatc tatttttatc
    34201 agcatttgca ataagtctta tctaacacaa aaaaacctga atgatgctcc ttcgcatcat
    34261 tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa
    34321 gaatcaagga ttcgcagggc tgccaggatt cgttggattg ttcggcgtgt taggcggaaa
    34381 aataggcgcc ttgtctaacg ttttttctag ttttgattct tcacgatcgt ctcgttctac
    34441 atcaggactt tcattcaatt tgaattcttt ttccatctca tctaactttt taaagtcatt
    34501 gccagattct tttgctaatt tcgctaattt atcttcggtt tctttagcca taaagtaacg
    34561 cccccctttt gatttcagct tagcatatca cactgaaaaa acaaaagaaa acgctaacca
    34621 acgatcattt ttagctattt ctcatcttta aaatcaagca ttttttgccg gtataaaatc
    34681 gtagggagaa ctgttttcca taccaatcat cggatctact aactggttat cgatcacttc
    34741 tgctgtaaga acgaaatctt tagaaacagc aggttctacc tcttctttct tttcttctcc
    34801 aacaaatgaa gaaaacagat ctggttccac tgtttccatt acttggtttt tcgtttcatt
    34861 cgtcccactg tcatcttcta caggtagctt ttcttttaca acccgtggct cttttgtata
    34921 gttcccatct tcaaatggat catttccgac caaagaatct tcataagctg ctagtttggt
    34981 ccggagagtg atattcattt tatcattatc catgatcctt gatttcaacg tagtcatccg
    35041 gcttgctgat tcattcgcta cacaagtgac ataggcttgc tcttccccta acaaaatcaa
    35101 cagttcctta ctgcgagtca ctgccgtgta gagcaaatta cgctgaagca tccgctgata
    35161 ttgatggacc atgggcaaaa taaccatctt gaattcactg ccttgtgatt tatggattga
    35221 gcaacaataa gaaagagtga ttttattcca ttcgtttctt ttataagtga cctcatttgc
    35281 atcaaactgg ataactaatt catccacttt atcttcagaa tcttttgcca agatgatacc
    35341 cgtgatttct cccatatctc cattgaatac attcaattcg ggagtattga ctaattggag
    35401 gactttgtct cctatccgat aaacggtgtc gttccaatgt acttcctttt ttccaggttt
    35461 ccctggattg aatatctcct gcatcatttt atttaacgca tcgatccctg caggtccccg
    35521 atacattggt gctaatactt ggatatcttg tgcacggaat cccttatttt tcgctttttc
    35581 cacaatttta gagatcaagg gttcaatttg ataagcatca cttcggaaat aggaacgatc
    35641 tttttgattc tttgtgaaat cttttggcag acgtccttct ttgatctcat gggcaagagg
    35701 aataatacta gaaccatcac cttgccgata aatctgattg agttccattt tgggaatttg
    35761 ttcgatttcc aaaagatcat ggagtacttg accaggaccg acagagggaa gctgatcttt
    35821 gtctcctact aagataactt gcatattcgt cggaatcgct ttgaataatg tgttagccag
    35881 ccatgtatcg accatagaca tctcatcaac aatcaaaagt ccgccttcta attctttcgt
    35941 actgactgaa ggtgtttttt cccgtccatt cagccccaga agacgatgga tcgtactggc
    36001 aggcagacct gtcgtttcgt tcattcgttt agctgctcgg ccagttggtg ctgcaagtag
    36061 aataggaaaa atttcttggg tatattttga aggatccaat tccagtccgt tcaactctgc
    36121 aaaaagattg acgattccgt tgatcacagt agttttcccc gtacctggtc ctcctgttaa
    36181 aataaataga ggggaacgga ttgcttcagc gatagcttgt tcttgcgaag gtccatactg
    36241 tatgccgaat attttttcga tacgccgaat attcttctgc acttcttttt caggataatg
    36301 gatttctttt ttacgactga gaaggcgctg gatcgaatta ccgatccccc attcagaaaa
    36361 gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt atctttcctt cttcaacaag
    36421 ttggataatt acagtagcaa cctgatcagg ctggatttca attgggcgac ttgtttctaa
    36481 aatacggatc gtttgctgaa gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac
    36541 tgcatgctga aaaatctgat gcaagatagc tgctcgaatt cttctcggtg aatctgctgc
    36601 aataccgatt tgttcggcta gattatctgc acgtttgaac ccgattcctt ctatgtcttc
    36661 gactagctgg tacggatttt cttcgatgat atccaaagac tcgttcttat aggcctggta
    36721 gatactgaaa gctaactgac taccgaaccc gtaacgattc agtcccatga tcacttgatc
    36781 catcccgtgg tttagccgaa tcgtctcagc aagcatttct ctttttggct tcgtcaaacc
    36841 ggagatttgt tttaataccg aaggatcagc aatgattttt tcgatagcat cttctcccag
    36901 caattctaca attttttctg ctgttttctt cccgattcct gggaattttt cgctggaaag
    36961 atacgctacg atcccatttg cagttgttgg cttttcttgc tcatatcttt cgagtttaag
    37021 ctgccttccg tatttaggat gatccactaa ttctccataa aaacgataag tctcattttc
    37081 ttgaatatcg ccgaaacttc cggtgactac aatctctttt tcacggtaat cagctgtggt
    37141 ttcactgatg tgtaccaaaa gcactttgta aaaattacta ggattttgaa aaaaaatcgc
    37201 ggcaactgta ccaacgaaat aaggcatttc ttctggctgc atcggaagcc gcctccttct
    37261 tctatagtaa aggttctggt gctgtttcag ttcctaaaaa atctgtttgg ttccatactt
    37321 tcagttcata tggcaataat ctgcaaggct cttcagtctc taaaatcgtc aaactactat
    37381 tgaacaatcc acccatttca cgcaactcac ttagctcttt tcctgtcatc cattgaatgg
    37441 ctgcagttaa agaagctcca tgtcctacga ataaaagtgg gccatcttta tgttgggcaa
    37501 ccgcatctgc tacaacagtt tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa
    37561 aaggagaagg atcatacaga tctaaatgat tacgcaaatt cggcagatta tccggatatt
    37621 tctggtacat ttcatcgatc gactgacctt ctaaagtacc cagccctagt tctcttagct
    37681 tatctgtata aaaaatctct accggacgag caagctcttt gttgatacct tctgctgtat
    37741 cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa aggaacatat tttacctttt
    37801 tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc
    37861 cttgaaaacg tctttcctga ttccattgtg tcttcccatg tcgggtaaaa tatagttcca
    37921 tacgcagcaa actcctgtct ttcttttatt ttactctata ataaactgtc taatccagct
    37981 tgtcattttc atagtgatgc gtcaattcta aattagggaa tccttgctgg cgaagtgctt
    38041 catacactat cattgcagct gtattcgata aattcaatga gcggacatgc tcatcattca
    38101 tagggatgcg taaacacttt tcttcgtttt cacgcataaa tgtttctggc aaaccggttg
    38161 tttcttttcc aaataaaaag aagtgctctt ctccatcagc aaaattttcg tcactatata
    38221 ctttatgggc aaattttgag atcaaatgta gtttacgatt acctaaaaat gttaaaaaag
    38281 cttctaaatt actgtggtaa gtaatattta catcattcca ataatccaac cctgcccgtt
    38341 ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat taaatgaagc ggagtattcg
    38401 tggctgcaca cgtacgggca atatttccag tgtttgcagg gattaatggt tcaaataaaa
    38461 cgatatgatt catcaggatc attcctctca tatttatgtt cgtttctatt ttctgacaat
    38521 ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt
    38581 tagtgaagct cttctcacta acttttatca gaacaggaac ctgataaaaa ccaatgaaaa
    38641 aacaacttgt tgctaatata acaccctcaa cttttttttg caacctctac tcattaaaaa
    38701 ctgctagaaa aacgttttct cttagttcct tttaataatt ctatgtcaat tgtaatcttc
    38761 ttcaaaggat tcttatataa gtctgctttg attcgctctt ctacctgcca ttctaaaggt
    38821 gacatggcca aaagatcttt ttgattttcc tctggtatct caaattcata ggttacacgc
    38881 tgacgttcag ttaatttcat tgcttcagca aacttggcta ctacacgttc attggaataa
    38941 ctctgttttt caggttgatt cggataaaaa gcctgacgta gttcctttaa ataattttct
    39001 tgtggcacca ccttcaacac ttctccatct tctgagagta cacgttggaa ttcttggtaa
    39061 tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg
    39121 gtcaaatcag ctacacacca aaatgcttcg gcagattgtt ctgtagctgc gtagactcct
    39181 tcttttgaaa tatcaaatcc aacagcattt tgaatctttt tcaattttga aagttcattc
    39241 aagaagctac cttctccgca gccaacatct aatagcgaga cttcttcagg taaatgctgc
    39301 gcaatagtct caataacggg ctgatacatg ccagattgga tcattctacg ccttggaacg
    39361 aacattcctt gatcgtattc tgttttaacc gcatgggata aaaaataaag tgttcctttt
    39421 ttggaaaggt caaaacggtg attatttttg catatcaagc cattatcaga cttttttact
    39481 tcttcttggc aaaccggaca gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct
    39541 cggtcaattt tttttaacat agttccccct catttctgtt ttatcttacc gttagatgct
    39601 cactttggca actgctaaaa atatgataca atgcctagaa gaaagtgaaa gataaaggag
    39661 aagattagat gatcaaagaa ttttgcgctg aaaactacac atctattcct ctagcaattg
    39721 ctagaggagc cgatcggatc gaattgtgtg acaacttagc ggtcggtggc acaaccccga
    39781 gtactggtgt gatcgaagaa acgttaagtt atgctgggga aaaagatatc cccgtaatga
    39841 cgattattcg tcctagaggg ggcgacttcg tctataacga tatcgaattg aaaatcatgc
    39901 atactgattt gattgaagca aagaaattag ggacggatgg cgtagtattt ggttgtctta
    39961 ctccttcagg ctggctagac gaagaggcct tagaactatt gatcgaaaca gcagaagggt
    40021 tgcagatcac cttccatatg gcttttgaca gtattcctga agatcggcaa ttcgaagcaa
    40081 tcgattggct ggtagaacat ggtgttgacc gtatcttgac tcatggcggt cctgctgaca
    40141 aaactattgt agaaaatttc ccacggctta aagaattgat tgcctataca gatggccgct
    40201 taatcatttt gcctggtggt ggtgtcactg cagagaatgc agcacatgta gctaaagaat
    40261 tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct tcagttataa tcttataaac
    40321 aaaaaaacat ttgcaacagg cataatgctt attgcaaatg tttttgtttt tttacataaa
    40381 atagcccata aataaagaag caatattgaa ataaatcagt acactcgtca aatcgctcaa
    40441 agtcgtgata aagggaccgc ttgctactgc tggatcaaat cctagacgat ccattagcat
    40501 cgggattaaa ctacctgcaa gattcgccac tgtgattgca aatagcatag ccatccctat
    40561 gacaaacccc aagataaaat tatgtttcca aaaacctacg attagaaaaa tcgtgatccc
    40621 cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc
    40681 atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc acagctagcg actgggttcc
    40741 agcattaccg gcagttcctg taatcagcga aatgaagact gctaaaatac tcgcttcact
    40801 taccaagtct tcatagtgac tgattaaaga agcggtcgac atacctaaaa acaacagagt
    40861 gatcagccaa ggcaaacgtc gagacgctgc ctttactgga ttttcattaa tttcttccac
    40921 attgaccccg gcaagtcctg aataatcgct agcagcttca tcatcaataa cgtcgatgat
    40981 atcatcgact gtgacgattc ctaacaaatg atcatcataa tcggtaacag gaagagctaa
    41041 gaagtcataa tctctaaatg tttgtgccac atcttcttgg tcgtcgccta catgaactga
    41101 aagcactcgt tcgctcatca gatcagaaat cattgcgtcg tcatcattca cgattaggtc
    41161 gcgcagagaa ataaccccta ctagctgacc ttcttgatta acgacataga tgtaatagat
    41221 tgtttcagct acatctgctt cgttcttcag cacgtacatc gcagagcgaa cagtttgatt
    41281 ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg
    41341 gagaagttcc ttgatttcac tggcatcgtc tgccgacatc aaacttaaat atttagcaat
    41401 ttgcttttta tccagcgtat taagcaagtc aacagcatta tctgtataca tctcagaaag
    41461 catatcagct gcatagctag gacgcatttc atctaggtat tctttcatat gttcgttgtc
    41521 ttcttcaatc acatcgaaca tatccgctaa ttctttaggc gacagatagg aatagatcag
    41581 ctgcctgttc tcttctgtaa gagattggta gaattgcccc tgctcataga tatgcatctc
    41641 taaaaaactg tttctgaatg cctgaatatt attttccctt aaatcttgag tcaactgggc
    41701 aaatctttcc tctaattctt gattttcatc caaaccaatc tcacctcgcc ttattcttca
    41761 aaccgtctca caactgcctc catgtctgcg gctaaaggct gcttgaactc cagcaactct
    41821 tctgtaaatg gatggtaaaa acgcagatca taacaatgaa gggcttgtct ttttattccc
    41881 agatccattc gcccgccata catatcatca ccgagcaggg cacagccaat tgcggaaaaa
    41941 tgaactctta tttgatgtgt ccttcctgta tgcagctgga tatcaactaa cgcgatttct
    42001 gttagtcgct tctctatcca gtactcagtc tgtgccgaac gtccttcatc agagaccatt
    42061 cgcttcaata gcgaagaata atctcgggcg atcggcaaat cgatatgtcc atgtgccgat
    42121 aaatctgaaa gccttccact gactaaggcc tgatattttt tcacaaactt cttttcacga
    42181 agttgcatat ctaattttgc gtgcgcaaat ccatgctttg caaaaagcat caaaccagat
    42241 gtatcccgat ctagtcgcgt caccacatgg attacttggt tttcgtaacc ttgtttttta
    42301 taataagcct ttacacgatt agccattgtc ccatttggat ggtattgggc aggaatagac
    42361 gaaacgcctg ctggtttatt tactaccaga acgtgttcat cttcatacac gatatcgatc
    42421 ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag caggaatcac aatggttacc
    42481 ttatcatcct tagctagaga aaacaagaca ttttctactt gatcattaac tttgatttga
    42541 ccgccttgaa attttatttt cgctaacaga ccctttgaga tccctttttc ttttaaaaaa
    42601 tattttactt gctgagaatg atctttctca taatcccatg taaactgcat atcaatcctc
    42661 actaataaag gcatctttca cacgatgcca aaaatgcata tgtcgataag aagcaaaatg
    42721 gatccgttct tctgctattc gataataaat tgagcggata tttgcttttg cgacattcaa
    42781 ttgatcaacc gttacaagat aatcttctgt attttccaat tttatttcta gccattcatc
    42841 atgggcaatc acgatcggag aacccaaagt acgaaacact cgattattca gtgaagcaat
    42901 ctctgccaac tgaaaggcat tgatactagg atggatcaca gcaccgccga cacttttatt
    42961 atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa
    43021 cagctcgttt ttgatgtata catcggctac catcgtacga ttggcccgtt taatcgttga
    43081 ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc aaataactga tccgaacatc
    43141 cagcaaggga taactaacac tttgttcccg gttcgttcgt aagctgtcta ctagttcttc
    43201 cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa
    43261 acgcacatca ttcagacaat gactgtagtg atggaacgcc gacaataatg tgccgtctcc
    43321 gccgactgag atgaccaatt ctggctcatg atcatctcgt tgaatccctg cttgttctaa
    43381 aagtgcgttc aaacgttctg tcacttctat agtctttgct tctttattat gaacgatcgc
    43441 tactttcatc ccctgcctcc tctctttcgc tttctttctt caaaagcgtt cgatagtaga
    43501 catcatctga ttttcctctt ccatgggaga ataaccgctg tgcttcttgg atttcttcac
    43561 ggatactgga catctcttca tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt
    43621 cactcatttc ttctggaaaa acgccttgat atttataatt cagcgaatgc tcaatcgttg
    43681 cccagaaatt catcgcaaga gtccgaatct ggatttccgc caaaattttc ttctcaccgc
    43741 tgatcagctg tacaggatac tcgatcacca gatgataaga acggtagcca cttgcttttt
    43801 tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc ttttctttta cggatgatct
    43861 caacaacttg atggatatct tctacaaact gacacatgat ccttaacccg gcaatatcag
    43921 acatttcttc ttctaaacgt cccattggaa tatgccgtaa ttgtgatttt gttacaatac
    43981 tatcgactgg cttgactctc ccagtgacaa attcaatcgg gacatgtcga ttttgttcac
    44041 ggaactgttt gcggatcccc cgcagtttca ctttcaattc tgatacagtt tgttcatatg
    44101 gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac ctctacttca ttaacattca
    44161 ttgtttattc taccatatat gcaaagtttt ttcgtactct tgatttatgc attgaccaaa
    44221 aaaagaaaat taggcaaaat agacaaaaag gagtcgatca aatgacacaa atgcttgaaa
    44281 tcgaatataa atctatgctg acaaagcaag agtacgagat attcatcgct cactatcaat
    44341 taacagataa agatttccgt gtacagacta atatttattt cgataccgtt gatgaacagc
    44401 tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg tcatcaagct gaatatacat
    44461 taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga tacctttata acagaagaag
    44521 cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt
    44581 ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg aaagcttaca acaaaacggg
    44641 ctgaattccc tattgaagaa ggacttttag ctattgatga aagctgggga gacaatctac
    44701 acgattttga actggaacta gaggtagggg atgcatctgt gggaaaaatt gcttttataa
    44761 attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc caacgtatgc
    44821 ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca tttttttact
    44881 tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata gaatctattt
    44941 tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg gaatactgtt
    45001 tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca ccatcaaaag
    45061 aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg gatgtcatgc
    45121 aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc tcaatgactt
    45181 attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa ggccgccgtt
    45241 ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct caagaacttg
    45301 ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa gatcgtcagt
    45361 ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa atgggcatca
    45421 caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt ggtgttttag
    45481 tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa acaacagata
    45541 actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag ttccattcaa
    45601 gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc ttacaagaga
    45661 gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc ttataaacga
    45721 tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc atcttcaata
    45781 tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg ataatcgctg
    45841 ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg gtttagtatt
    45901 ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac atagtaatta
    45961 taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc ttcgactgct
    46021 ggaccataat attttgcatt caatttacca taactatcac ttaggtattc actagtcaat
    46081 ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg
    46141 cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc ccgtacacga
    46201 gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt tgtcgaagca
    46261 atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga acgagtgaaa
    46321 taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc cagcgtatca
    46381 tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata agcaccgctt
    46441 cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt ttctacgatg
    46501 gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt tgcttcttcg
    46561 tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata catgtgcagt
    46621 ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc
    46681 ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt attgctcaaa
    46741 gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg tccttttata
    46801 tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact gtacaatccc
    46861 ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc atacacgcca
    46921 tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac tagatctgca
    46981 ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc tccagcaaga
    47041 agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg ctggatataa
    47101 tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg gtcatctgac
    47161 aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga aaacagactg
    47221 cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc gtttttaagg
    47281 tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca
    47341 tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga ttgttttaac
    47401 tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa gatcttggtc
    47461 aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt tacttccatg
    47521 aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag tctaacatcc
    47581 acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc aattttagca
    47641 taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata cttactattt
    47701 tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca tctcttcatc
    47761 aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc atgagttgaa
    47821 atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg
    47881 ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata gatgcaaaag
    47941 atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt gcttgtgaat
    48001 aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt ttttcatttg
    48061 gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag aaaaagtcca
    48121 gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat gatacaaata
    48181 aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc tattatagta
    48241 gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat taccaagtat
    48301 ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa atctctggtg
    48361 agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat ccggcctttg
    48421 ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc ccagccaatc
    48481 catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa agaccgaaca
    48541 gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt tcaaaatcac
    48601 tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt tagcaaaaca
    48661 gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact tatttacgtc
    48721 taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat caagtaataa
    48781 gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga
    48841 tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt tgtcacagtc
    48901 tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag cattacgcgc
    48961 catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct tttcagcaaa
    49021 ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt ccatccagaa
    49081 gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag cagactctag
    49141 ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg
    49201 tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc tgccaataat
    49261 ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt ctgattgaga
    49321 aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc cttttggcaa
    49381 aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa cttcttccaa
    49441 aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca aaaatgaaac
    49501 gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt cattaatatg
    49561 ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga ttcattgtag
    49621 cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca
    49681 gcggattttt caacctttca agctatctta tttaactata acatttttgg cagacgaata
    49741 ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat catcatcttc
    49801 tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa aacttggaaa
    49861 aactatcttt gtaatagt
//
LOCUS       DAFFQY010000010        48262 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFFQY010000010 DAFFQY010000000
VERSION     DAFFQY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.010"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            125..1168
                     /locus_tag="LRB02_000509"
     CDS             125..1168
                     /locus_tag="LRB02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL4658742.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="LRB02_000510"
     CDS             complement(1230..1694)
                     /locus_tag="LRB02_000510"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL4658743.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="LRB02_000511"
     CDS             1940..2887
                     /locus_tag="LRB02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4658744.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="LRB02_000512"
     CDS             complement(3098..5170)
                     /locus_tag="LRB02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4658745.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="LRB02_000513"
     CDS             complement(5103..5480)
                     /locus_tag="LRB02_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL4658746.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6187)
                     /locus_tag="LRB02_000514"
     CDS             complement(5609..6187)
                     /locus_tag="LRB02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4658747.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="LRB02_000515"
     CDS             6319..7611
                     /locus_tag="LRB02_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4658748.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="LRB02_000516"
     CDS             complement(7671..8789)
                     /locus_tag="LRB02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL4658749.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LRB02_000517"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LRB02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL4658750.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LRB02_000518"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LRB02_000518"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL4658751.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="LRB02_000519"
     CDS             11995..12594
                     /locus_tag="LRB02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL4658752.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LRB02_000520"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LRB02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL4658753.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFRMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="LRB02_000521"
     CDS             complement(14289..14633)
                     /locus_tag="LRB02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL4658754.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15915)
                     /locus_tag="LRB02_000522"
     CDS             complement(14770..15915)
                     /locus_tag="LRB02_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4658755.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="LRB02_000523"
     CDS             complement(16095..17069)
                     /locus_tag="LRB02_000523"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL4658756.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="LRB02_000524"
     CDS             complement(17149..18024)
                     /locus_tag="LRB02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL4658757.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="LRB02_000525"
     CDS             18154..20061
                     /locus_tag="LRB02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL4658758.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="LRB02_000526"
     CDS             complement(20602..23880)
                     /locus_tag="LRB02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL4658759.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="LRB02_000527"
     CDS             complement(24151..24696)
                     /locus_tag="LRB02_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4658760.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="LRB02_000528"
     CDS             complement(24708..25400)
                     /locus_tag="LRB02_000528"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL4658761.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="LRB02_000529"
     CDS             complement(25419..25703)
                     /locus_tag="LRB02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658762.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="LRB02_000530"
     CDS             complement(25716..26279)
                     /locus_tag="LRB02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4658763.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="LRB02_000531"
     CDS             26507..27169
                     /locus_tag="LRB02_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL4658764.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="LRB02_000532"
     CDS             27293..28489
                     /locus_tag="LRB02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL4658765.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="LRB02_000533"
     CDS             complement(28590..29966)
                     /locus_tag="LRB02_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4658766.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="LRB02_000534"
     CDS             complement(30003..30869)
                     /locus_tag="LRB02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL4658767.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="LRB02_000535"
     CDS             complement(30958..31983)
                     /locus_tag="LRB02_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4658768.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="LRB02_000536"
     CDS             complement(32001..32675)
                     /locus_tag="LRB02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4658769.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="LRB02_000537"
     CDS             complement(32859..33752)
                     /locus_tag="LRB02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4658770.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="LRB02_000538"
     CDS             complement(33826..35523)
                     /locus_tag="LRB02_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4658771.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="LRB02_000539"
     CDS             complement(35516..36829)
                     /locus_tag="LRB02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL4658772.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="LRB02_000540"
     CDS             complement(36829..40347)
                     /locus_tag="LRB02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL4658773.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="LRB02_000541"
     CDS             complement(40640..41098)
                     /locus_tag="LRB02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4658774.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(41396..41914)
                     /locus_tag="LRB02_000542"
     CDS             complement(41396..41914)
                     /locus_tag="LRB02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL4658775.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(41916..42533)
                     /locus_tag="LRB02_000543"
     CDS             complement(41916..42533)
                     /locus_tag="LRB02_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL4658776.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42530..43384)
                     /locus_tag="LRB02_000544"
     CDS             complement(42530..43384)
                     /locus_tag="LRB02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4658777.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43381..44292)
                     /locus_tag="LRB02_000545"
     CDS             complement(43381..44292)
                     /locus_tag="LRB02_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658778.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44333..45667)
                     /locus_tag="LRB02_000546"
     CDS             complement(44333..45667)
                     /locus_tag="LRB02_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4658779.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            45875..46705
                     /locus_tag="LRB02_000547"
     CDS             45875..46705
                     /locus_tag="LRB02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL4658780.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(46949..47231)
                     /locus_tag="LRB02_000548"
                     /pseudo
     CDS             complement(46949..47231)
                     /locus_tag="LRB02_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(47389..47583)
                     /locus_tag="LRB02_000549"
     CDS             complement(47389..47583)
                     /locus_tag="LRB02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658781.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13937 a        10308 c         8574 g        15443 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgttaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttatcatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg cattcggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct tttctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgattgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgattgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    41281 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    41341 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    41401 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    41461 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    41521 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    41581 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    41641 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    41701 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    41761 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    41821 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    41881 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    41941 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    42001 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    42061 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    42121 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    42181 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    42241 atctgatagt cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    42301 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    42361 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    42421 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    42481 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    42541 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    42601 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    42661 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    42721 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    42781 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    42841 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    42901 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    42961 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    43021 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    43081 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    43141 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    43201 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    43261 atgaagtaaa caccatccaa agcaatagaa tgatcaaaat gattcctatt actgtcatga
    43321 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    43381 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    43441 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    43501 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    43561 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    43621 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    43681 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    43741 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    43801 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    43861 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    43921 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    43981 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    44041 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    44101 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    44161 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    44221 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    44281 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    44341 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    44401 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    44461 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    44521 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    44581 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    44641 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    44701 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    44761 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    44821 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    44881 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    44941 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    45001 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    45061 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    45121 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    45181 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    45241 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    45301 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    45361 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    45421 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    45481 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    45541 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    45601 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    45661 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    45721 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    45781 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    45841 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    45901 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    45961 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    46021 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    46081 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    46141 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    46201 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    46261 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    46321 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    46381 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    46441 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    46501 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    46561 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    46621 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    46681 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    46741 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    46801 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    46861 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    46921 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    46981 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    47041 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    47101 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    47161 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    47221 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    47281 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    47341 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    47401 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    47461 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    47521 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    47581 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    47641 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    47701 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    47761 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    47821 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    47881 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    47941 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    48001 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    48061 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    48121 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    48181 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    48241 gatggttagg aatcctttag aa
//
LOCUS       DAFFQY010000011        47859 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFFQY010000011 DAFFQY010000000
VERSION     DAFFQY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.011"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(37..1188)
                     /locus_tag="LRB02_000550"
     CDS             complement(37..1188)
                     /locus_tag="LRB02_000550"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL4658782.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="LRB02_000551"
     CDS             complement(1201..1638)
                     /locus_tag="LRB02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4658783.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="LRB02_000552"
     CDS             complement(1653..3419)
                     /locus_tag="LRB02_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL4658784.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="LRB02_000553"
     CDS             3683..4360
                     /locus_tag="LRB02_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658785.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="LRB02_000554"
     CDS             4363..5490
                     /locus_tag="LRB02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4658786.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="LRB02_000555"
     CDS             complement(5690..6130)
                     /locus_tag="LRB02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4658787.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="LRB02_000556"
     CDS             complement(6141..8174)
                     /locus_tag="LRB02_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4658788.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="LRB02_000557"
     CDS             complement(8176..9879)
                     /locus_tag="LRB02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4658789.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LRB02_000558"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LRB02_000558"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL4658790.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="LRB02_000559"
     CDS             complement(10962..11987)
                     /locus_tag="LRB02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL4658791.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="LRB02_000560"
     CDS             complement(12196..13065)
                     /locus_tag="LRB02_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL4658792.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="LRB02_000561"
     CDS             complement(13524..15416)
                     /locus_tag="LRB02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658793.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="LRB02_000562"
     CDS             complement(15413..17140)
                     /locus_tag="LRB02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4658794.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="LRB02_000563"
     CDS             complement(17156..17806)
                     /locus_tag="LRB02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL4658795.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="LRB02_000564"
                     /pseudo
     CDS             18010..18306
                     /locus_tag="LRB02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18478..19098
                     /locus_tag="LRB02_000565"
     CDS             18478..19098
                     /locus_tag="LRB02_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL4658796.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="LRB02_000566"
     CDS             complement(19190..20356)
                     /locus_tag="LRB02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4658797.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="LRB02_000567"
     CDS             complement(20368..20721)
                     /locus_tag="LRB02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL4658798.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="LRB02_000568"
     CDS             20849..21871
                     /locus_tag="LRB02_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL4658799.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="LRB02_000569"
     CDS             21890..22051
                     /locus_tag="LRB02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658800.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="LRB02_000570"
     CDS             complement(22310..22744)
                     /locus_tag="LRB02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658801.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="LRB02_000571"
     CDS             complement(22854..24713)
                     /locus_tag="LRB02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4658802.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="LRB02_000572"
     CDS             complement(24831..25991)
                     /locus_tag="LRB02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL4658803.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LRB02_000573"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="LRB02_000573"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL4658804.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="LRB02_000574"
     CDS             complement(27738..28436)
                     /locus_tag="LRB02_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL4658805.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="LRB02_000575"
     CDS             complement(28393..30054)
                     /locus_tag="LRB02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL4658806.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="LRB02_000576"
     CDS             30272..31672
                     /locus_tag="LRB02_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL4658807.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="LRB02_000577"
     CDS             complement(31924..33003)
                     /locus_tag="LRB02_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4658808.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="LRB02_000578"
     CDS             complement(33089..34048)
                     /locus_tag="LRB02_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL4658809.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="LRB02_000579"
     CDS             complement(34060..35571)
                     /locus_tag="LRB02_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL4658810.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="LRB02_000580"
     CDS             complement(35593..36156)
                     /locus_tag="LRB02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL4658811.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="LRB02_000581"
     CDS             complement(36162..37025)
                     /locus_tag="LRB02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4658812.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="LRB02_000582"
     CDS             complement(37039..37935)
                     /locus_tag="LRB02_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658813.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLFDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="LRB02_000583"
     CDS             complement(38110..39399)
                     /locus_tag="LRB02_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4658814.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="LRB02_000584"
     CDS             complement(39371..40888)
                     /locus_tag="LRB02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL4658815.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="LRB02_000585"
     CDS             41086..41976
                     /locus_tag="LRB02_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL4658816.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="LRB02_000586"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="LRB02_000586"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL4658817.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="LRB02_000587"
     CDS             complement(42885..44357)
                     /locus_tag="LRB02_000587"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL4658818.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="LRB02_000588"
     CDS             complement(44994..45356)
                     /locus_tag="LRB02_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL4658819.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46046
                     /locus_tag="LRB02_000589"
     CDS             45579..46046
                     /locus_tag="LRB02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4658820.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKRLQHPMKNG"
     gene            46043..46330
                     /locus_tag="LRB02_000590"
     CDS             46043..46330
                     /locus_tag="LRB02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HBL4658821.1"
                     /translation="MNMDKETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKK
                     WLSYSWPEYQPKAHKGLGMMYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46470..47393)
                     /locus_tag="LRB02_000591"
     CDS             complement(46470..47393)
                     /locus_tag="LRB02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4658822.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47408..47680)
                     /locus_tag="LRB02_000592"
     CDS             complement(47408..47680)
                     /locus_tag="LRB02_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658823.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13932 a         9944 c         8259 g        15724 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttaatata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaaacaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaggt
    46021 tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag aaatgcaaac
    46081 tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag aagatgaaag
    46141 cttagcagac cgtatcttcc aagctcataa gaagtggtta tcttacagct ggcctgaata
    46201 tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac ggttcactgc
    46261 atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa
    46321 aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac ctaataacaa
    46381 aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc cattctttca
    46441 caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata aggtattttc
    46501 actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca agcttccaga
    46561 tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc gcgtccaatc
    46621 accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac catatccgaa
    46681 tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg ttttcctttc
    46741 tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa aagttgatga
    46801 catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc caacgtgatg
    46861 cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat tgattgttct
    46921 cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa aactagaggt
    46981 ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt aatgatcaat
    47041 ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc tttctgatag
    47101 cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat gatcccactg
    47161 atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat gcttttcgtt
    47221 tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc tttcatgacc
    47281 gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat tacacgtgaa
    47341 atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc cactgctgct
    47401 tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga ccgcattcaa
    47461 aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc caatatatat
    47521 cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc ctattcaacc
    47581 cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca taaaaataat
    47641 tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct ccttttgata
    47701 ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc caaagaaaag
    47761 ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac attataatga
    47821 tactgtaaat atatctttat tattagtgaa tggatattt
//
LOCUS       DAFFQY010000012        47113 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFFQY010000012 DAFFQY010000000
VERSION     DAFFQY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.012"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(137..1528)
                     /locus_tag="LRB02_000593"
     CDS             complement(137..1528)
                     /locus_tag="LRB02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL4658824.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="LRB02_000594"
     CDS             complement(1741..2127)
                     /locus_tag="LRB02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL4658825.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="LRB02_000595"
     CDS             complement(2183..2806)
                     /locus_tag="LRB02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL4658826.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="LRB02_000596"
     CDS             complement(2870..3538)
                     /locus_tag="LRB02_000596"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL4658827.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="LRB02_000597"
     CDS             complement(3550..3990)
                     /locus_tag="LRB02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658828.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="LRB02_000598"
     CDS             complement(4049..4720)
                     /locus_tag="LRB02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4658829.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="LRB02_000599"
     CDS             complement(4905..5303)
                     /locus_tag="LRB02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL4658830.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="LRB02_000600"
     CDS             complement(5300..6073)
                     /locus_tag="LRB02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL4658831.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="LRB02_000601"
     CDS             complement(6070..6753)
                     /locus_tag="LRB02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL4658832.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="LRB02_000602"
     CDS             complement(6920..7807)
                     /locus_tag="LRB02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4658833.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="LRB02_000603"
     CDS             complement(7804..8673)
                     /locus_tag="LRB02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4658834.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="LRB02_000604"
     CDS             complement(8810..10096)
                     /locus_tag="LRB02_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4658835.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="LRB02_000605"
     CDS             10369..10947
                     /locus_tag="LRB02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4658836.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="LRB02_000606"
     CDS             10947..11876
                     /locus_tag="LRB02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4658837.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="LRB02_000607"
     CDS             11873..13609
                     /locus_tag="LRB02_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL4658838.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQVSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LRB02_000608"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LRB02_000608"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL4658839.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="LRB02_000609"
     CDS             complement(14505..15014)
                     /locus_tag="LRB02_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL4658840.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LRB02_000610"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LRB02_000610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL4658841.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="LRB02_000611"
     CDS             complement(16315..17406)
                     /locus_tag="LRB02_000611"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL4658842.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LRB02_000612"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LRB02_000612"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL4658843.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LRB02_000613"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LRB02_000613"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL4658844.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LRB02_000614"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LRB02_000614"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL4658845.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="LRB02_000615"
     CDS             complement(21369..22343)
                     /locus_tag="LRB02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL4658846.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="LRB02_000616"
     CDS             complement(22324..24207)
                     /locus_tag="LRB02_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL4658847.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="LRB02_000617"
     CDS             complement(24225..26450)
                     /locus_tag="LRB02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL4658848.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="LRB02_000618"
     CDS             26565..27599
                     /locus_tag="LRB02_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4658849.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="LRB02_000619"
     CDS             complement(27666..28679)
                     /locus_tag="LRB02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4658850.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="LRB02_000620"
     CDS             complement(28757..28945)
                     /locus_tag="LRB02_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL4658851.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="LRB02_000621"
     CDS             complement(29385..29513)
                     /locus_tag="LRB02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL4658852.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LRB02_000622"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LRB02_000622"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL4658853.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVKAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="LRB02_000623"
     CDS             complement(31000..32055)
                     /locus_tag="LRB02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL4658854.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LRB02_000624"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LRB02_000624"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL4658855.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LRB02_000625"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LRB02_000625"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL4658856.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LRB02_000626"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LRB02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL4658857.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LRB02_000627"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LRB02_000627"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL4658858.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LRB02_000628"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LRB02_000628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL4658859.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="LRB02_000629"
     CDS             39817..41376
                     /locus_tag="LRB02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL4658860.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="LRB02_000630"
     CDS             complement(41487..43418)
                     /locus_tag="LRB02_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4658861.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LRB02_000631"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LRB02_000631"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL4658862.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="LRB02_000632"
     CDS             complement(44347..45099)
                     /locus_tag="LRB02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4658863.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45259..45468)
                     /locus_tag="LRB02_000633"
     CDS             complement(45259..45468)
                     /locus_tag="LRB02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658864.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45479..46807)
                     /locus_tag="LRB02_000634"
     CDS             complement(45479..46807)
                     /locus_tag="LRB02_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658865.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            46943..47098
                     /locus_tag="LRB02_000635"
     CDS             46943..47098
                     /locus_tag="LRB02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658866.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT        13144 a        10183 c         8397 g        15389 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa cgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag taagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagctgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccttta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagagggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
    45241 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
    45301 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
    45361 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
    45421 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
    45481 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
    45541 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
    45601 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
    45661 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
    45721 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
    45781 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
    45841 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
    45901 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacgggt tgcatttgtg
    45961 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
    46021 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
    46081 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
    46141 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
    46201 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
    46261 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
    46321 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
    46381 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
    46441 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
    46501 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
    46561 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
    46621 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
    46681 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
    46741 gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
    46801 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
    46861 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
    46921 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
    46981 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
    47041 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
    47101 taagctcaaa aaa
//
LOCUS       DAFFQY010000013        44390 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFFQY010000013 DAFFQY010000000
VERSION     DAFFQY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.013"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(5..586)
                     /locus_tag="LRB02_000636"
     CDS             complement(5..586)
                     /locus_tag="LRB02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4658867.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="LRB02_000637"
     CDS             complement(769..1395)
                     /locus_tag="LRB02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4658868.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="LRB02_000638"
     CDS             complement(1417..2748)
                     /locus_tag="LRB02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL4658869.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="LRB02_000639"
     CDS             complement(2903..3304)
                     /locus_tag="LRB02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL4658870.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="LRB02_000640"
     CDS             complement(3390..3596)
                     /locus_tag="LRB02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658871.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="LRB02_000641"
     CDS             complement(3691..5193)
                     /locus_tag="LRB02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL4658872.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="LRB02_000642"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="LRB02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL4658873.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="LRB02_000643"
     CDS             complement(5764..6381)
                     /locus_tag="LRB02_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL4658874.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="LRB02_000644"
     CDS             6602..7975
                     /locus_tag="LRB02_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4658875.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="LRB02_000645"
     CDS             8050..9162
                     /locus_tag="LRB02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL4658876.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="LRB02_000646"
     CDS             complement(9234..10871)
                     /locus_tag="LRB02_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL4658877.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="LRB02_000647"
     CDS             complement(11039..11647)
                     /locus_tag="LRB02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4658878.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="LRB02_000648"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="LRB02_000648"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL4658879.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="LRB02_000649"
     CDS             complement(13201..13953)
                     /locus_tag="LRB02_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658880.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="LRB02_000650"
     CDS             complement(14094..15044)
                     /locus_tag="LRB02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL4658881.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="LRB02_000651"
     CDS             complement(15045..15884)
                     /locus_tag="LRB02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4658882.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="LRB02_000652"
     CDS             complement(15881..16627)
                     /locus_tag="LRB02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4658883.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="LRB02_000653"
     CDS             16865..18235
                     /locus_tag="LRB02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4658884.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="LRB02_000654"
     CDS             complement(18290..19186)
                     /locus_tag="LRB02_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4658885.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19300..19470)
                     /locus_tag="LRB02_000655"
     CDS             complement(19300..19470)
                     /locus_tag="LRB02_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658886.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(19467..20144)
                     /locus_tag="LRB02_000656"
     CDS             complement(19467..20144)
                     /locus_tag="LRB02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL4658887.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="LRB02_000657"
     CDS             complement(20235..21140)
                     /locus_tag="LRB02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL4658888.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="LRB02_000658"
     CDS             21315..22769
                     /locus_tag="LRB02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4658889.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="LRB02_000659"
     CDS             22785..23816
                     /locus_tag="LRB02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL4658890.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="LRB02_000660"
     CDS             23823..25010
                     /locus_tag="LRB02_000660"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL4658891.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="LRB02_000661"
     CDS             25166..26548
                     /locus_tag="LRB02_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL4658892.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="LRB02_000662"
     CDS             26520..26906
                     /locus_tag="LRB02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658893.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="LRB02_000663"
     CDS             complement(27106..27774)
                     /locus_tag="LRB02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL4658894.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27963..28760)
                     /locus_tag="LRB02_000664"
                     /pseudo
     CDS             complement(27963..28760)
                     /locus_tag="LRB02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29041..30420)
                     /locus_tag="LRB02_000665"
     CDS             complement(29041..30420)
                     /locus_tag="LRB02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4658895.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30595..31764)
                     /locus_tag="LRB02_000666"
     CDS             complement(30595..31764)
                     /locus_tag="LRB02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4658896.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(31739..32890)
                     /locus_tag="LRB02_000667"
     CDS             complement(31739..32890)
                     /locus_tag="LRB02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL4658897.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(32850..34211)
                     /locus_tag="LRB02_000668"
     CDS             complement(32850..34211)
                     /locus_tag="LRB02_000668"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL4658898.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELIEMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34208..35209)
                     /locus_tag="LRB02_000669"
     CDS             complement(34208..35209)
                     /locus_tag="LRB02_000669"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL4658899.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35225..35920)
                     /gene="dapD"
                     /locus_tag="LRB02_000670"
     CDS             complement(35225..35920)
                     /gene="dapD"
                     /locus_tag="LRB02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL4658900.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(35926..36642)
                     /locus_tag="LRB02_000671"
     CDS             complement(35926..36642)
                     /locus_tag="LRB02_000671"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL4658901.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36639..37520)
                     /locus_tag="LRB02_000672"
     CDS             complement(36639..37520)
                     /locus_tag="LRB02_000672"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL4658902.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37523..38323)
                     /gene="dapF"
                     /locus_tag="LRB02_000673"
     CDS             complement(37523..38323)
                     /gene="dapF"
                     /locus_tag="LRB02_000673"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL4658903.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFGRYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38310..39569)
                     /gene="lysA"
                     /locus_tag="LRB02_000674"
     CDS             complement(38310..39569)
                     /gene="lysA"
                     /locus_tag="LRB02_000674"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL4658904.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLIEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRRLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(39784..39954)
                     /locus_tag="LRB02_000675"
     CDS             complement(39784..39954)
                     /locus_tag="LRB02_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658905.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            complement(40055..40618)
                     /gene="efp"
                     /locus_tag="LRB02_000676"
     CDS             complement(40055..40618)
                     /gene="efp"
                     /locus_tag="LRB02_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL4658906.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            41046..41963
                     /locus_tag="LRB02_000677"
     CDS             41046..41963
                     /locus_tag="LRB02_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL4658907.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            41976..43121
                     /locus_tag="LRB02_000678"
     CDS             41976..43121
                     /locus_tag="LRB02_000678"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL4658908.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            43292..43750
                     /locus_tag="LRB02_000679"
     CDS             43292..43750
                     /locus_tag="LRB02_000679"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL4658909.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            43909..44379
                     /locus_tag="LRB02_000680"
     CDS             43909..44379
                     /locus_tag="LRB02_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL4658910.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT        12445 a         9206 c         8036 g        14703 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28021 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28081 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28141 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28201 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28261 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28321 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    28381 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    28441 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    28501 cgatggatcc gatttttggc tactgctcca acttttcctc gataagaatt gtagcgacgt
    28561 gatttacgcg tgtatgccgt tacttttatt cctagcttct tgatcaagcg ctgaactttc
    28621 tttttattga tagagaaacc gcggtttctt agttcattgg tcatccgtaa acagccataa
    28681 tccttgtgct tctcccgaat tttaagcatt tccgcttcaa tctcttgatc tggattttta
    28741 cggtccaatc gcctttgcca tccaaataat catgaacgat ctttaattta agttcaaaag
    28801 tatatttaac cataaaaaat accgacctcc aaaagttaga ttttttggtc taacttttgg
    28861 gggtcggtac acaaatcgtg tcacagtccc ttttttgaaa ataaacggtg ttcaaaagct
    28921 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctgttt ttacttggct
    28981 gttcttatta cttgatgcaa ggcagctttt tcacaacctc ttttcaaata tttcgttttc
    29041 ttattctaca aatggttctt ctcctaacgc ttgttgagcg gctagattca acatgctcca
    29101 ttgtcggtca aagcctggct ggaagaagaa gtcagcttct gctagttctt ctaaagtcag
    29161 tttgttttgg atcgctagtg ccaatacatt tccttgacct gtaacatcat aggtagataa
    29221 tactgctcct cctagcaatt gatgggtcat tggatccgag aataattgga tggacacttt
    29281 tggatttcct tgttcttctg gtacatattt tggtcgtaac gtatcttcat aataagacac
    29341 tcgtacatct actcctgctt ttgctgctgt aaagctattc aatccgcttg ctgcaaaatg
    29401 atagtcaaag acacttaatg cagaggaacc tacgactccg ccgaacggtt tgtttggtac
    29461 ttcttcaaaa agatgtttta ctacataacg tgcttctcta cgtgcaacag tcgctaaagc
    29521 gatcggcatt tttttgcgtg ctgggataga ataagcaagt gtagcatctc ctactgcata
    29581 cacatctgga agattcgttt gcaagtattc atttgtttgg atccagccac gttcatctaa
    29641 gtcgacgatc ccttttaacc attctgtatt tgccttcaca ccggcagctt gaatgactag
    29701 atctgctggg atttcttttt cacttgtttt gattgcctca acttttccgt taccagtaaa
    29761 agcttcgatt tttgcatttg tcaaaacttc aataccgttt tcatttaggt gttgttctaa
    29821 aatatctgtc atctcagcat ctaaataagt gcctaaaggt ctatcgatca catcaagcaa
    29881 tacgacttct ttcccagctt ttcggcttgc ttctgctgct tcgatcccaa tgtaacccgc
    29941 acctacgaca gcgatttttt taatagccgg atcatttaat ttttctttga ttttggttgc
    30001 ccaattgtat ccacgcatca agtagacatt ttctagatct gctcctggta ctggcagatt
    30061 gtttggtttg acaccagaac tcaaaatcaa tttgtcataa ggatattctt ttgtttcgtt
    30121 atctgatgcg ctagtaaccg ttactgtttt tttatcggca tcgatcgttt ttacttcgtg
    30181 attatttaaa atatgaacat tggattgcgg aaagttttct ggtctgaaat tacgtacatc
    30241 atttacatct gttaccttgt cttctaaata cagctccata ccacatgaca tgaaggaaat
    30301 atagtctcct gcttcaaata gcgtgatatc tacattttca ccgtatcgtg tcaatagttc
    30361 caagatcgat tgatgccctc catgagatgc acctacaatg acaactttga ttttttccat
    30421 tgatgaaacc tccttgaata ttcttttaaa atcattctaa acaatttaaa cttaacatta
    30481 cattagttat gaatcaaaag aaacgcgttc atttgaacta aatttaaaaa acaatcgtct
    30541 ccttgcagaa tataccgcaa aattaacgat tgtttttttg ataaaatcta tgaatcactg
    30601 ttctgttaaa aagtctttga tccgtttgca ggcctcatgt aatttttcca tgctgctagc
    30661 ataagaaatc cggatataac cttctccttc tgggccaaat gcaattcctg ggatacacgc
    30721 aacttttgcc tgttccgcta attctacaca aaactgccaa gagtcagttt gaatggcagc
    30781 tgggagtttg caaaacaagt aaaatgcacc gtttggctgg gtcacttcaa atcctaaagg
    30841 cgccaattgc tggaccagat agtctcttcg tgtttgatat tgctctttca tattctcgct
    30901 atctttcacc ccatttatta gcgcttcagc cgcagctttt tgcgacatcg ttgtagcggc
    30961 agtcaccaga tattgatgga ctttaattag ttcgtcaata agggcttttt gagcaaaaat
    31021 aaagcccact cgccaaccag tcatcgcatg tgacttggat aacccgctta tcacgattgt
    31081 ttgttctgga agataagtag ctatggacat atgcgttcca ctataagtca attcagcata
    31141 tacttcatca cttataacaa ataccggata tttttttaaa acctcagcga ttgctttcaa
    31201 ttgttttgca gacaatgtcg tcccagtagg attgttagga tagtttaaaa tgactgcttt
    31261 gacagctgat ccatattgtt caaatgctcg ttcaaattgt tccggctgac agacaaaccc
    31321 tgtgtcagaa gtatctatcg ggatcaattc agcaccagct agttccacta atggctgata
    31381 tcctgaataa gcaggcgctg gaataagaac cttgtctcct tcttccaaaa cagataacaa
    31441 cgcagaggca atcgcttccg ttgcaccaac tgtcgtcaaa acttccgttt gcggatcata
    31501 agttaatcca taacgctgtt gctgaaatat acaagccgct tcccgcagtt cctctaatcc
    31561 tctcattccg gtatagtgag aaaagtcttc ttcaatcgct gctattcctg cttgcttgac
    31621 atgttcagga gttggaaaat ccggttctcc cagggtgagt ttgatcacat ctggtatcga
    31681 cgatatttgc tgatcgaatt gtcgtatttt tgatacttct attctagaaa gcctaggact
    31741 aaatcgattg gataaattca tcattattta ctcgctcctc ttttgaactg ctatcgattt
    31801 ttacaagaag attttctgta aaaagaatcg ttttgtacca ttctacacca ttttctaaaa
    31861 catccatatc aaaatcgaac gtcggatcat gtaaagcagg ggtatcaccg acacctaaaa
    31921 agaaaaatac acatggatat tgttgcgtat atatgccaaa atcttcggct tgaagaactg
    31981 gagcatctaa ttcatgtagt ttgtttgaat ctaacagtgc tgcatataag cctctatggt
    32041 tgattacagc tggatagccg tcattatagc gaaaagaaat agtagctgta gagtgaaact
    32101 gttctgctaa atggtctaat cccctcttca aataattttg catatctgaa ctgtaagaac
    32161 ggatacttcc ggatacaata acttctccag ctaaaacatt tcggatcgtt ccaccttgga
    32221 tttggccaaa tttcattaga tgattccact ctgccggtaa ttgccggtca aaatcataaa
    32281 cattcttcaa aaatcgtaca gctgcctcca gactatcgat tcctttttca ctagaagcaa
    32341 tatgtgcaga ctggccttta atgataatgt ctgtttcact gctttgtgcc atcaaaggtc
    32401 ctggtcgaga gaaaacttct cccttagcta gtccaggcca tagatgaaag ccaaatattg
    32461 cttgtacttc atatttatcc aacaatcctg actgaataac gctttgtgcg ccaccggaaa
    32521 tttcttctga tggctgatag atcagtacta cattataaat aagatcctca atgtgtttag
    32581 ttataaagtc agtcaaccct aaaagcatcg ccatatgtcc gtcatgacca catgcatgca
    32641 tataaccttc atgcttagaa gcaaaagaga gattcgtttc ttctttgact ggcagtgcat
    32701 cgatatctgt tcgaaaagca atcgtttctt cttgctgatt atcaaaataa aggagtagtc
    32761 ctgtttcgcc gatttcatgg ataacggcct cgctgttttt tacttgttca tataaatatt
    32821 tttttgtctt gaattcatga aacccgattt caggaatttg atggagtgcc attcgatatt
    32881 ctttcagcat ccgtattcac catcccttca tagataccct tcactactgt ttcatattga
    32941 ttattcggta caccgatgat cagattcaac tcctgtgctc cttgtgataa gatcgatgtg
    33001 cgaatttcta atttgttcaa tatcgataac actttcccag ataatccaat caattcttta
    33061 tgcggacccc caacgacaga gatcaaagct aagtcttcga tcacgacgat ttcttccacg
    33121 ccaagtgttt cttttaattc tttcgtgatg agaaataact tatcggcaat cgcttctgct
    33181 gagacgatca ctccgatatt gtcgatccct gaaggaatat gttcgattga tatctcatgt
    33241 ttagcaaaaa tgctcattgc tttccagata aacccgattt catcggacat atgacgtttg
    33301 aataaagtga tggaaagaaa atccttgcgt ccagctattc ctgtcagtcc attttccttt
    33361 tctatttcgt gattcgatat ttttgtccct tgagcgaaag gatcattggt attttttatt
    33421 tggatgggga tatcagcctc ttgaactggg aaaacagctg cttcatgaag aacgttagcc
    33481 cccatataag ccatctctat cagttctcta aaagagattt cctcgatgat tttaggatga
    33541 tcgatgattc gcggatcagc catcatgatt cctgaaacat ccgtccaatt ctcataactt
    33601 tccgcttgca acatcttcgc cagtatcgcg ccggtaatgt cgcttccccc acggcccaat
    33661 aatttttctt ttcctgcagg gttcgccccg taaaaccctg gaacaaccat tttcggatat
    33721 cttttaaatg cttttgtgat ttcctgtgct gttttttcct gatcgatttt cccatcatag
    33781 tcaaagaaaa tcaggtcttt tgcgtcaata aactggtaac caagatactc agccatcagt
    33841 tgagcagtca gatattcacc tctacttact aaataatctt cagttatctt ttcgtcttcc
    33901 agttgtttct gtatgcgtgc tagttcatct tcaataggta actgcagggc aagaacatct
    33961 cttacttgaa tgaaacgatc acaaatcctt ttccaaaggg gtgtccaatc aacatgatgc
    34021 tgaaggtaag cataaatcaa ataaagtaag tctgttactt tgtcatcatc tgacgtacgt
    34081 ttgcctaaag cagaaacaac gatcacttgg cgattgggat ctgaaagaac aattgtacgg
    34141 acttttttga attgctcttc atgagacatc gatgttccgc cgaatttcgc tactttcagc
    34201 gtattactca tcttgccacc tccgctattt tctgcattaa agctgcagca atctgtacgg
    34261 cattcgcagc ggctcctttg cgtatgttat cggcagtcgt atagaacaac aatccgttct
    34321 ctaaagaagt gtcttttctg atcctcccaa cataaaccat atctgttcct ctagcaagaa
    34381 tgctcgtcgg atatttatgt gctgctagat catcttctaa ttgaatccct ggaaactggc
    34441 tgagacactc tctaatttct tccgtcgtaa actctttttg cagttgaact gccacagaga
    34501 ctccatgtcc attttcgatt ggcacacgta cgcaggttgc tgatactggc aaagcaggct
    34561 gatgcagaat cttacgggtc tcatttatca ttttctgttc ttcttttgta tacccatttt
    34621 ccaaaggctg atcgatttcc ggaatcaccg tttggctaat attataagga tacaaaactg
    34681 gttcctcgtc attttgcgta cgatgaagat cttcaatgcc tttttgcccg gaaccagaaa
    34741 ctgcttgata ggtcgaataa ttgactcgct ggataccaaa ggcttcttgt aaagctttaa
    34801 gcggaagtac cgcttgaatg gttgaacaat ttggatttgc aatcagccga ttcaatgtat
    34861 agtcttccag attgacctct ggaacgatta atggaatatc cgaatcttcc cgccatgctg
    34921 aggaattatc tatgacaaat agtccctgac ttcgtgcgat tggcgcaaac tctttggata
    34981 cagaagcacc tgcagaaaat agcgctaaat catatccttc aaatgaagtt tctgtcaatt
    35041 cctgtacagt gtgtttcttt ttcttgaata cacaaacttt accggctgaa cgttttgaag
    35101 ctaataattt caatcgtcct actggaaaat catattcttc taacactttg agcatcgttt
    35161 ggccgactaa tccggttgcc cccacaatac aggtatctat ttcgcgcatt tttctccatc
    35221 tcctctataa ttcgcgtaac gcagcaatca atgctgtatt ttcttttgtc tgatcatcac
    35281 ttatttttaa tacacgtgca ggtattccac cgacaacagt ttctgctgcc acatcttcta
    35341 aaaccactgc tcctgctgcc acgactgcat cttttccgat atggacacct tcaacaatca
    35401 ctgcattcgc acctaccagt acgccgtctt ctacgataac cggtttggcc gatgctggtt
    35461 cgatcacacc tgctaagaca gctcctgcac caatatggca gtttttgccc actgtcgctc
    35521 gtccacctaa gacagcaccc atatcaatca tcgtgttttc accgattact gcaccaatat
    35581 tgataatcgc tcccatcatg atcacggcat tgttgccgat tgacacttga tctctgataa
    35641 tagcacctgg ttcgatacga gcattaattt ttttaatatc tagcaaagga actgcgctgt
    35701 atcttgcagc agtttcgatt tcgatttcaa tgattttttc tgattcagct tctaaaaccg
    35761 gagcaatcgt ctcatagtca ccaaaaacaa cgaagctgct tccttcacca aaaactttac
    35821 tttctttaaa ttcgattggc tcggttacat tcagatagac tttcacaggc gtctttttag
    35881 atgccgtttg aatgtagcgg atgatttctt gagcttccac gttttttaca cccctttgtc
    35941 cattaataaa tcttctaatt tgtaataacc tggtgactga tctttcaacc agtccgctgc
    36001 ttctactgcg cctgcagcaa agattcgatt agaaaaagat tcatgtttga tcgtaaatat
    36061 ttcatcagta gcagcaaaca atacatcatg ttcaccaggt aaagaacctg cacgaatact
    36121 gtgtacacca atttggtttt cttctctttt tttcccttgc cagttgtata cgggctgcag
    36181 cttttgcact tcttggattg tttgaagcag catatttgca gtaccagatg gtgcatcttt
    36241 tttacgacta tgatgttttt caatcagttc gatctgccag ccttgcaaca ttgcagctgc
    36301 ttgcttgatt agttgattca tcaccatgat gccgaacgaa aaattagcac tatacatcac
    36361 aggtacatgt ttggcatgtt cttttatttc ttggaactgt gattgtgtat aacctgtcgt
    36421 gccaatgact aacggaagtt gatatgataa gctatacgaa aggattttct ctaaattatc
    36481 tggatgagaa aaatcaataa gcagttctgg cagttccgta aaagcaagcg tagttgcttc
    36541 tttaggtgcc gtccgtaaaa gaaggaccgt atctcctctt tcttgtgcaa cttcttcgac
    36601 tctatttccc atctgtccac tgccaatcag tccgattttc atttttcaac cgtcctttct
    36661 tttaacaaaa gctgagctaa ttgtttcttt ttctcttttg aaagagaaac taaaggcagg
    36721 cgtagaggtc cggaaggtaa gtctaataat gacatggctg ctttgacagg aataggattg
    36781 gtctcactga acaaggcatg gatcagttca agtgcattta attgcttttc ttttgcttgc
    36841 tgccatcttc cgtcctgcgt atcttttact aattcatgga ctattttagg ttggatattc
    36901 gcccatacac ttattacacc gcttcctcct acactcatta ctggtaggat caaatcatca
    36961 tttccactgt aaaggaagaa ttcttctcca atcaaccgag cggcatccat cacataagcc
    37021 atatctcctg aggcttcttt caaaccaatg atattaggat gttctgctaa atttactaat
    37081 acatggattg gaatcgacat gcctgttcta gaaggaacat tatacaaaat aagcggaatc
    37141 ggtgaggctt ctgcaattgc tgtaaaatga gcagctagtc ctgcatcact tgttttgttg
    37201 tagtaagggg tgatgaccaa aagtgcgtct gcccccaaag aagcaaaatg cttcgctttt
    37261 tcaatcgttt ctttcgtatt attcgttcca gctcctgcaa tcaccggcac acggccagcc
    37321 actttcttta ctgtcagttg aaggatctgc tcctcctcag attgtgtgag cgtagaagat
    37381 tctcctgttg tacctaaaac taataatcca tctgtttgtt catctaaatg gaattggatc
    37441 aatttttcca aaccagcata atctactgat ccctcttcat tcatcggcgt gatcagcgcc
    37501 acgatcgaac cttttatcat cttcattcct ccaatattct tgtccattca tgaactgctg
    37561 ggccagccat ttgtatcgca tcctcaccag agatcatcaa gtctccttgt tccaagtgaa
    37621 caactacctg atctgctgaa attttcccag tttccttagc aaccacataa gaggcacagc
    37681 aacctgtacc gcaagcaagt gtccaaccta ctcctctttc ataggtacgg acgatcaatt
    37741 cttgttcatt gactacttct acaaagttga cattggtttt ttctttgaac aaaggatgat
    37801 gacagatcag ttcacctctt tttctccaga cttcctcacg cgcattttca acaaaaacaa
    37861 cggtatgaat cgttcctaaa aataaactag tgatcgttac tttttcacca ttgatctcta
    37921 tttcctggtt ttttattgga taagtttgat tagctttgat tttttccgga gaatcatctg
    37981 gcactcccat atctacgctg caataaaaag gtgagggatc caacaccgta atttttcttg
    38041 tgcctgctaa tgtctctacg ttgaattcct gttcattcat catgttctga tcatttacgt
    38101 atcgaccaaa acagcgaatg ccatttccgc acatcggcgc gcggctgcca tctttgttgt
    38161 aatagatcat ttccaacgga tcgattttta cggcgatcaa tccgtctata tcatattttt
    38221 cttttgtgca taaagaagcg gctaattgac tataatctgt gtcttctgcg taatctaata
    38281 agataaaatc atttccgcag ccattatatt tatgaaggat cataggaaac ttcccccctt
    38341 agtagatcag aaaatgcttg tctgcgaaca atcagtcttg attggccatt attgacaaaa
    38401 acaactgctg gtcgtgtcgc tcgattgtag ttgctgctca tcgagtatcc ataagctccc
    38461 gtggaataaa cagctaaaag atcacccgat tctgctttcg gcaaataagc ttctttaatt
    38521 accacatcgc ctgattcaca catttttcct gcaatcgtca ctttctctgt tttttcttcc
    38581 gacagttttg ctgctaaatc acaatcgtat tctgcctgat acagagctgg acggatatta
    38641 tctgtcatcc cgccatctac aaagtaatat tttttatgtg gtgtcttttt gatgaaacca
    38701 atcgtgtata gtgttgtacc agcttctcca acaatacttc tacccggttc gatcagtaat
    38761 tgacgaatgg tcagtcttcg tttttccaaa gcttcttccg tatattcaat cagttttttt
    38821 agtgcctctt cgactgggat cggctgatca ttttgtgtat aacgaatccc aaaccctccg
    38881 ccaagattca aggaaagagg ttggtcaaaa tcggttaagt aattcgtcaa tttgtcgatt
    38941 tcagctaacc aagcagtaac atcaaaaatt tgtgacccga tatgagcatg aaatccttct
    39001 aactctagat agctgctttc ttttatcaac tgaagggctt gttggcactc tttactttca
    39061 tagctcatgc cgaattttga atcaatatgc gcagtgacga tatattcatg agtatgtgct
    39121 tctactccga cattcaaccg aagcatgata caaatttttt ttaaatagcg ttggcttaat
    39181 tctgctaata attcaacttc aatcaaatta tcggcaacaa tatgcaacaa atcattttca
    39241 agtgcataga tcagttcctc tggcgtcttg ttgttaccat ggaagtagat agtatctcta
    39301 ggaaaatcag cttgcaaagc ggtatagatt tccccaccac tgacaacatc tagcccgaat
    39361 ccatattcac gaactaaatg cagcatctca atcgtttgga atgctttaga ggcgtaaagc
    39421 acttttgtat caaaacgttt cgatacaaag cctcgcttga atgacagcat cgtttgtctc
    39481 atcttttctt catcatacac atacagaggt gtcttatact tttctgccaa tgtgcttgct
    39541 gtgactccgc caattatcaa ttctgtcatt ttttcattcc tcttttcact ttttttccgt
    39601 ttttattgga atttcatttg ataaagattt gaattttatt ggaataatat taaacaaaat
    39661 tttctgaatt gtctagtata tttcgaaata tttttttaaa aataagaata aaaatggtta
    39721 taaaaacaaa atacccgaac tattagaagc tatttataca taaaaacata aattcaacac
    39781 tatttagtga tgaaaaaata ttttcgcttc aatatagttc ggaatagttc ggatattttc
    39841 aattttatga atcatttcaa tttcttcata aataagtgca gttttttctt tctctagctt
    39901 ttgccattca tctttcaaaa tataaataaa atcaaatact tttttatttt tcaatttgct
    39961 gattccacaa aaaacctctt gaaaggcaga tccgatgatc tgcctttcaa gaggtcaagc
    40021 tctgttttca aattcttatc aatccgttag cgaattattt tgacgcacgt tttacataag
    40081 aaccttctgc tgtattgatt tccaacaatt catctgtttc gatgaagtca ggaacattga
    40141 ctactaagcc agtttccatt gtcgctggct tacctgaacc agtgactgtt gcacctttga
    40201 tggaaggttg tgtttccaca acacgtaata cgacagttgt tggtaattga acgccgatga
    40261 cttcgctacc gaaaaattgg atttttactt ccatgttttc taggatatat ttcatttctt
    40321 cttctactgt ttctacagga atttcatatt gctcatatgt ttccaaatcc atgaagaaag
    40381 ctgtatcatc catcgtgtac aaatattgta caggttttgt gtcgatatgt gctttttcaa
    40441 atttttcttc tggtctgaac gttgtgtcgt atgttgcacc tgaacggaca tctttcagtt
    40501 tcatacgcat aactgtattt cctttaccag gtttatggtg gcttgcatcc agaactttaa
    40561 tcagtttgcc gtcttgtacg aaagtcatac cggctcttaa atcacttgct gagatcatta
    40621 tttttgctcc ttcttttctt tatcataatc aactttattg tatcagacaa ccggccagaa
    40681 aactagagta ttctctatct tttaaggtct catgcctatt tgtcagaaaa ataaacacgg
    40741 gtaaacgatg ctatcgaaat atttttttct ttcaagcctt tgacttttat tttttttcat
    40801 gctatcgtta ataaaaattt tacttggagg gatacacatg acagtcagca accaaaaaca
    40861 attgaatcgt tgctgttgta tggtgcataa aagcagcata ctatttgtca tgtctattaa
    40921 ttgcccttca tagaatgagt accaggtaac ttatcttttt atctactcaa tttttctcta
    40981 tgtctttcaa ggagctgatt taatagtcag ctcctttttt tattatcttt ttttaggagg
    41041 cccacatgat tatctcgaca attacagaag ctattggttc cacaccgctt tatcgctttg
    41101 acaatcagca ctattctatg cctaatggtt ctgctattta tgcaaaacta gaatatttaa
    41161 atcctggagg aagtatcaaa gatagattgg gcagttattt gatcaaagaa ggatttgccc
    41221 aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac tgccggaaat acaggaattg
    41281 ggatcgctct ggccgcactc acatacaaat taagaacggt ttttgttgtt ccagagaagt
    41341 ttagcttaga aaaacaacaa ttgatgaaag cattgggagc aaagatcatc cattctccta
    41401 gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa acttgcagag gagatatcaa
    41461 acagctatct gcctcttcaa tttgaaaata aagataatcc tgctgcttac tatcatacgt
    41521 tagggccaga aatctttcat gaaatcaaag aaaatatcca tagttttgta gcaggaattg
    41581 ggagtggtgg aacctttgct ggaacttcta cctttttgaa agaaaaatat cccgacattc
    41641 gaataatcgg tgtagaacca gagggctctg tgttaaatgg tggtgatcca gcccctcacg
    41701 aaattgaggg tatcggtgta gaattcattc cgccgtttct ttctcccctt tcaatcaatc
    41761 agatcgagac aatctctgat gtagaagggt ttaactatac taggcagtta gcacgagaac
    41821 aaggactatt agttggaagt tcgagtggtg cggcttttgc agcagcatta agagaaattc
    41881 gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc agacgcagca gaccgctatt
    41941 tatcaaaaaa catttactta taggaggaaa gaaacatgca cattcaaaca aaattgatcc
    42001 acggaggcat cagtgaagat cccacaacag gagctgtcag tgtacctatt tatcaaacat
    42061 ccacttatcg gcaagatggc gtcggccagc ctaaacaata tgaatattca agatctggta
    42121 atcctactcg ttttgcattg gaagaactga ttgctgattt ggaaggcggt gttcgaggat
    42181 tcgctttttc ttctggtctt tctgggattc atgccgtttt ttctcttttt caagctggcg
    42241 atcatatctt attaggagat gatgtctatg gaggaacatt ccgtctattt gacaaagttc
    42301 ttactaaaaa tggtctcgag tacacgatca tagatacgag taatcttgat aaaatagaac
    42361 aatccatcaa acccaataca aaagcacttt atcttgaaac acctagcaat ccgcttctaa
    42421 agattactga tttagaaaaa agtgccactc tggctcatca acatggattg atagttattg
    42481 ctgacaatac atttgctact ccttattttc aaagaccttt agatctcggt tctgatatcg
    42541 ttgtccatag cggtaccaaa tatctaggcg gacacagcga tgtagtagct ggtttagtga
    42601 cttctaacca taaagatcta gccgatcaga tcggatttta ccaaaatgct atcggagccg
    42661 tacttggtcc ccaagatagc tggttacttc agcgcggaat caagacacta agtgttcgta
    42721 tggaggaaca tcaaaaaaat gcttttgtgg tagctgattt tctattttct catccagcag
    42781 tagaaaaggt ctactatcca ggattacccg atcatgaact ccatggagtt gctaagcagc
    42841 agatgagcgg ctttagcgga atgatttctt ttactttaaa aaatgaagaa tctgctattc
    42901 catttgttga atcactccaa ctatttactt tagcagaaag tttaggtggc gtagaaagtt
    42961 tagtggaaat tccttctgtc atgactcatg cgtctattcc aaaagaaaaa cgagaagagg
    43021 ctggcataaa ggacggcttg atccgtcttt ctgtaggaat tgagtatggt caggatctca
    43081 taaacgattt agctcaggca tttgacagaa tcaagaacta gaagtcccaa taaacgactt
    43141 agcagaaaaa tctaaaaaat atttcagatt gctacacctt ggaaacgata agtgttagaa
    43201 tcaagtagaa agacgttttt gtaaatttca ctaaataatt ctttagtgaa aagcatcaaa
    43261 acgaatgaac aaacagatag gagtcttaca aatgagaatg aatgtcgaaa gttttaatct
    43321 tgaccataca aaagtcaaag caccttatgt acgtgtagct gatcgaaaaa cgggtgcgca
    43381 tggcgatgtc atcattaaat acgacgtccg ctttaaacaa ccaaataaag aacacatgga
    43441 catgccaagt ctacattcac ttgagcactt gactgccgaa ctaatccgca atcacgcaga
    43501 ttacatcgta gactggtctc caatgggatg ccaaacaggc ttttacttga ctgtattgaa
    43561 tcatgaaagc tacgaagaca ttttagatgt tcttgaagca acaatgaaag atgttcttga
    43621 agctactgaa gtacctgcaa gcaatgaaac acaatgtggc tgggctgcca gccatacact
    43681 agaaggtgca caagctttgg ctcgtgaatt tttagcaaaa cgcgatgaat ggtctgaagt
    43741 agaagcatag tttctaaaag aacgtaaaga tgagaagcaa tatagctatc ttatcttcgt
    43801 attttattaa atatttattg tctgaaaaga gttctaaacg ggaaaacaaa tagctagttt
    43861 tcagtttagc gctcttttta ttatataaaa tagaaaggag aaaactttat gtctatttat
    43921 aattttgaaa ctactttaga agacggcact gtctattcat tgaaaaaata tgcaggcaaa
    43981 ccgataatca ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt taaacaatta
    44041 gaagaacttt accaagctta tcagacacaa ggtctggtta tcttaggctt tccttccaat
    44101 caatttaaac aagagctaga ttcagcgact gaagcagcag aagcttgtaa aaccacttat
    44161 ggcgttacct tccctatgca tcaattgatt gacgtaaatg gagaaaatac agatcctctt
    44221 tttacttatc ttaaaaatga agctcctggt actgtcacta aagcaataaa atggaatttt
    44281 actaaatttt tgattgatag aaatggcaaa gtaatcgaac gatttgcacc acaaacttct
    44341 ccattgaaaa tgaccgctgc aattgaatcc gttctttaac ttaataaaaa
//
LOCUS       DAFFQY010000014        41208 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFFQY010000014 DAFFQY010000000
VERSION     DAFFQY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.014"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(26..328)
                     /locus_tag="LRB02_000681"
     CDS             complement(26..328)
                     /locus_tag="LRB02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4658911.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="LRB02_000682"
     CDS             complement(353..2446)
                     /locus_tag="LRB02_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4658912.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="LRB02_000683"
     CDS             complement(2935..3699)
                     /locus_tag="LRB02_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL4658913.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LRB02_000684"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="LRB02_000684"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL4658914.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="LRB02_000685"
     CDS             complement(4704..5780)
                     /locus_tag="LRB02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL4658915.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="LRB02_000686"
     CDS             complement(5804..6397)
                     /locus_tag="LRB02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL4658916.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LRB02_000687"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="LRB02_000687"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL4658917.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="LRB02_000688"
     CDS             complement(8067..8366)
                     /locus_tag="LRB02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL4658918.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LRB02_000689"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="LRB02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL4658919.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="LRB02_000690"
     CDS             complement(10121..11032)
                     /locus_tag="LRB02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL4658920.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="LRB02_000691"
     CDS             11152..12009
                     /locus_tag="LRB02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4658921.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="LRB02_000692"
     CDS             complement(12196..12558)
                     /locus_tag="LRB02_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL4658922.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="LRB02_000693"
     CDS             12904..14343
                     /locus_tag="LRB02_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658923.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="LRB02_000694"
     CDS             complement(14350..16902)
                     /locus_tag="LRB02_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL4658924.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="LRB02_000695"
     CDS             complement(17016..18347)
                     /locus_tag="LRB02_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4658925.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19790)
                     /locus_tag="LRB02_000696"
                     /pseudo
     CDS             complement(18313..19790)
                     /locus_tag="LRB02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(20095..21930)
                     /gene="tdc"
                     /locus_tag="LRB02_000697"
     CDS             complement(20095..21930)
                     /gene="tdc"
                     /locus_tag="LRB02_000697"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL4658926.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22400..23827
                     /locus_tag="LRB02_000698"
     CDS             22400..23827
                     /locus_tag="LRB02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658927.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23919..24284)
                     /locus_tag="LRB02_000699"
     CDS             complement(23919..24284)
                     /locus_tag="LRB02_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL4658928.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24281..24646)
                     /locus_tag="LRB02_000700"
     CDS             complement(24281..24646)
                     /locus_tag="LRB02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL4658929.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24838..25086)
                     /locus_tag="LRB02_000701"
     CDS             complement(24838..25086)
                     /locus_tag="LRB02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658930.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25085..25213
                     /locus_tag="LRB02_000702"
     CDS             25085..25213
                     /locus_tag="LRB02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL4658931.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25270..26964
                     /locus_tag="LRB02_000703"
     CDS             25270..26964
                     /locus_tag="LRB02_000703"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL4658932.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26968..27087
                     /locus_tag="LRB02_000704"
                     /pseudo
     CDS             26968..27087
                     /locus_tag="LRB02_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27738..28871
                     /locus_tag="LRB02_000705"
     CDS             27738..28871
                     /locus_tag="LRB02_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL4658933.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28902..29222
                     /locus_tag="LRB02_000706"
     CDS             28902..29222
                     /locus_tag="LRB02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL4658934.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29251..29715)
                     /locus_tag="LRB02_000707"
     CDS             complement(29251..29715)
                     /locus_tag="LRB02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4658935.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29846..31103)
                     /locus_tag="LRB02_000708"
                     /pseudo
     CDS             complement(29846..31103)
                     /locus_tag="LRB02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(31132..31449)
                     /locus_tag="LRB02_000709"
     CDS             complement(31132..31449)
                     /locus_tag="LRB02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4658936.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31475..31795)
                     /locus_tag="LRB02_000710"
     CDS             complement(31475..31795)
                     /locus_tag="LRB02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4658937.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32259..33674)
                     /locus_tag="LRB02_000711"
     CDS             complement(32259..33674)
                     /locus_tag="LRB02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4658938.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33691..35007)
                     /locus_tag="LRB02_000712"
     CDS             complement(33691..35007)
                     /locus_tag="LRB02_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4658939.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35157..35855)
                     /locus_tag="LRB02_000713"
     CDS             complement(35157..35855)
                     /locus_tag="LRB02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL4658940.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36144..37100)
                     /locus_tag="LRB02_000714"
     CDS             complement(36144..37100)
                     /locus_tag="LRB02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4658941.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37213..38046)
                     /gene="msrA"
                     /locus_tag="LRB02_000715"
     CDS             complement(37213..38046)
                     /gene="msrA"
                     /locus_tag="LRB02_000715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4658942.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38221..39855)
                     /locus_tag="LRB02_000716"
     CDS             complement(38221..39855)
                     /locus_tag="LRB02_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658943.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39842..41176)
                     /locus_tag="LRB02_000717"
     CDS             complement(39842..41176)
                     /locus_tag="LRB02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658944.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12504 a         8475 c         6960 g        13269 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttc caattctaaa tggtcgattc
    18601 aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata
    18661 cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc
    18721 agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg
    18781 gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta
    18841 gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca
    18901 ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg
    18961 caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca
    19021 tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat
    19081 actccgcgaa tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt
    19141 tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta
    19201 gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca
    19261 actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct
    19321 aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg
    19381 atgatccaat aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc
    19441 acattatcga taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca
    19501 ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca
    19561 ttcgataccc aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag
    19621 atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc
    19681 cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt
    19741 ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct
    19801 tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac
    19861 aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta
    19921 gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag
    19981 aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa
    20041 ataattgtct gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg
    20101 gatgatttcg tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa
    20161 aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg
    20221 ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc
    20281 accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc
    20341 gccatccata taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa
    20401 atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa
    20461 tggatagact tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct
    20521 gaatctttga gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa
    20581 tggcaataca cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc
    20641 caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc
    20701 gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata
    20761 agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct
    20821 gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc
    20881 aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga
    20941 acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt
    21001 taacctttct cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt
    21061 tcctacgaca gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt
    21121 tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag caaccatctg
    21181 atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg
    21241 gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac
    21301 ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa
    21361 attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc
    21421 taaaggaatc gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc
    21481 atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc
    21541 tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt
    21601 ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg
    21661 tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac
    21721 ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc
    21781 tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc
    21841 atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat
    21901 aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat
    21961 gtcttgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa
    22021 tttttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    22081 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    22141 atagataacc taaaaaaagc gaacaaacaa ataaaatgac tgtccttcat tcactaaaca
    22201 tatttttatt tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt
    22261 tttccaaatt ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa
    22321 ttaactgatg atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat
    22381 acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct
    22441 gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct
    22501 cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga
    22561 agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca
    22621 tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg
    22681 tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat
    22741 ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg
    22801 gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct
    22861 taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga
    22921 acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga
    22981 aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc
    23041 tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt
    23101 cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt
    23161 tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga
    23221 agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt
    23281 tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc
    23341 gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt
    23401 ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag
    23461 ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt
    23521 gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac
    23581 aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga
    23641 ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca
    23701 gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga
    23761 aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa
    23821 caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt
    23881 tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg
    23941 ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct
    24001 agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca
    24061 agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg
    24121 ctgactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt
    24181 gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt
    24241 ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc
    24301 tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga
    24361 taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga
    24421 tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc agttttcttg
    24481 tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa
    24541 gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa
    24601 tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact
    24661 ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta
    24721 aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct
    24781 ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta
    24841 gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct
    24901 aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac
    24961 aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat
    25021 ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt
    25081 tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc
    25141 tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt
    25201 cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga agacattgga
    25261 ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca
    25321 gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca
    25381 ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg
    25441 aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt
    25501 aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag
    25561 ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac
    25621 aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat
    25681 aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa
    25741 ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac
    25801 ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg
    25861 cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc
    25921 aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa
    25981 gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca
    26041 ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa
    26101 gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca
    26161 gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca
    26221 gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa
    26281 ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat
    26341 ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca
    26401 tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctatcgtc
    26461 gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat
    26521 ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga
    26581 aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat
    26641 attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg
    26701 ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct
    26761 aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc
    26821 tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt
    26881 cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt
    26941 gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta
    27001 ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg
    27061 cagatgcaag cttttgaaca ccgtttacca agaggttgtg aaaaagctgc cctgcatcaa
    27121 gtaataagaa cacccaagct aaaactgctt cttatgtttt tgacttggtt ggacttatta
    27181 ccgcagatgc aagcttttga acaccattta ccaagaggtt atgaaaaagc tgccctgcat
    27241 caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta
    27301 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag
    27361 tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac
    27421 ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt
    27481 ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat
    27541 acaactattt caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca
    27601 aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt
    27661 aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc
    27721 tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca
    27781 gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa
    27841 cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag
    27901 ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa
    27961 cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt
    28021 tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg
    28081 ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa
    28141 gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa
    28201 aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag
    28261 taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa
    28321 aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc
    28381 tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag
    28441 ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag
    28501 gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg
    28561 gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag
    28621 taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg
    28681 atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa
    28741 tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag
    28801 aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt
    28861 tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa
    28921 atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc
    28981 gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga
    29041 ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct
    29101 atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga
    29161 acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat
    29221 gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg
    29281 agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata
    29341 aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt
    29401 tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa
    29461 cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc
    29521 cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc
    29581 cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt
    29641 tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca
    29701 gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca
    29761 agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaac ggaaaacaat
    29821 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
    29881 tcgtagctac cttgacgaac gggagataga tcaaaataga aagaaccaaa ttgacagcgg
    29941 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
    30001 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
    30061 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
    30121 taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt
    30181 ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac
    30241 ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga
    30301 taaacggtgc gctatgccca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat
    30361 tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga
    30421 atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg
    30481 gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa
    30541 ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg
    30601 cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc
    30661 ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt
    30721 ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg
    30781 cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag caagaaagca
    30841 atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat
    30901 gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg
    30961 atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga
    31021 atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc
    31081 ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt
    31141 caatcgacga tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat
    31201 cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc
    31261 ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt
    31321 gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc
    31381 atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg
    31441 atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt
    31501 ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag
    31561 gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc
    31621 ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata
    31681 cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta
    31741 ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa
    31801 acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc
    31861 gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa
    31921 aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca
    31981 caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag
    32041 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    32101 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    32161 tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata
    32221 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    32281 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    32341 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    32401 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    32461 tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct
    32521 tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct
    32581 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    32641 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    32701 acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg
    32761 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    32821 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    32881 aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    32941 tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca
    33001 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    33061 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    33121 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    33181 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    33241 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    33301 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    33361 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    33421 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    33481 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    33541 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    33601 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    33661 atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc
    33721 tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat
    33781 aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca
    33841 agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt
    33901 tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg
    33961 cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt
    34021 gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt
    34081 gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg
    34141 tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg
    34201 ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc
    34261 aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg
    34321 tacgacattg taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc
    34381 cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga
    34441 aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca
    34501 gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc
    34561 tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc
    34621 ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc
    34681 atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa
    34741 gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg
    34801 caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt
    34861 gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat
    34921 gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc
    34981 cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt
    35041 gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca
    35101 aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa
    35161 ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc catatggaca
    35221 gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta
    35281 tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca
    35341 attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca
    35401 atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa
    35461 gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc
    35521 gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc
    35581 atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta
    35641 tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc
    35701 cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca
    35761 ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca
    35821 gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat
    35881 gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa
    35941 ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg
    36001 ataaaattca ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc
    36061 aataacagac gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga
    36121 taaaaagttt gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct
    36181 ggtgagaaat ctaaattatc catcattcga acactttctt gtaactgact taaccgactt
    36241 gctccgacca gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc
    36301 gctaatgatt gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt
    36361 gatccaactt gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca
    36421 ggaataccat gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt
    36481 gcccctagcc cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa
    36541 aacatattgt agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca
    36601 gtcatttttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    36661 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    36721 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    36781 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    36841 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    36901 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    36961 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    37021 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    37081 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    37141 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    37201 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    37261 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    37321 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    37381 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    37441 cgctaattct ttttgtcgtt cgccttgata aaaaatcact ggacgatatt gcttccctct
    37501 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    37561 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    37621 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    37681 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    37741 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    37801 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    37861 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    37921 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    37981 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    38041 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    38101 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    38161 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    38221 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    38281 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    38341 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    38401 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    38461 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    38521 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    38581 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    38641 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    38701 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    38761 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    38821 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    38881 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    38941 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    39001 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    39061 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    39121 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    39181 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    39241 aataaaaata aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta
    39301 cactagcaaa ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga
    39361 gatgatttga taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc
    39421 aattgcggaa cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact
    39481 aaataaaaat gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag
    39541 tgttgtacac aagttcaata accaaacgta aataatatag cttacaatca aattttttgt
    39601 catccaatat aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc
    39661 atatccagta taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc
    39721 cttgattcga gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt
    39781 gaaaaagatg aaagaatata tactactaac tataattaat ccactcaata ttactacttt
    39841 tttatttatt ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca
    39901 acttattagg tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac
    39961 tgatccataa agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt
    40021 ctccagcatt tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt
    40081 taccgtttac ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct
    40141 ttttgttgct tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt
    40201 cccacgattc tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt
    40261 ctgttggggc gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt
    40321 cttcattgat aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg
    40381 ctgctataga cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt
    40441 ttttattcat agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt
    40501 ggatacctaa gatacgccaa ggaaactgga gcaagcttat cggtgttttt tgaaacaaaa
    40561 accatggaaa taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt
    40621 tttctttttt ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta
    40681 ataaccccat agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa
    40741 tagaaagtgc ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca
    40801 tctgctgcaa cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct
    40861 ttactaaatt cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac
    40921 gaactataat catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat
    40981 acacaagtag acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca
    41041 atccataagc ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg
    41101 cggatcgata atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata
    41161 aaaaagcagc atctatcttt ttaaaaaact tttctccata ataaaaac
//
LOCUS       DAFFQY010000015        40649 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFFQY010000015 DAFFQY010000000
VERSION     DAFFQY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.015"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            75..521
                     /locus_tag="LRB02_000718"
     CDS             75..521
                     /locus_tag="LRB02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4658945.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(580..1041)
                     /gene="mscL"
                     /locus_tag="LRB02_000719"
     CDS             complement(580..1041)
                     /gene="mscL"
                     /locus_tag="LRB02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL4658946.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1293..2561
                     /locus_tag="LRB02_000720"
     CDS             1293..2561
                     /locus_tag="LRB02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL4658947.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2554..3840
                     /locus_tag="LRB02_000721"
     CDS             2554..3840
                     /locus_tag="LRB02_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL4658948.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4579..5502
                     /locus_tag="LRB02_000722"
     CDS             4579..5502
                     /locus_tag="LRB02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4658949.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            5711..6289
                     /gene="pgsA"
                     /locus_tag="LRB02_000723"
     CDS             5711..6289
                     /gene="pgsA"
                     /locus_tag="LRB02_000723"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL4658950.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6529..7770
                     /locus_tag="LRB02_000724"
     CDS             6529..7770
                     /locus_tag="LRB02_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL4658951.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            7879..8928
                     /gene="recA"
                     /locus_tag="LRB02_000725"
     CDS             7879..8928
                     /gene="recA"
                     /locus_tag="LRB02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL4658952.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9339..10895
                     /gene="rny"
                     /locus_tag="LRB02_000726"
     CDS             9339..10895
                     /gene="rny"
                     /locus_tag="LRB02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL4658953.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10974..11780)
                     /gene="proC"
                     /locus_tag="LRB02_000727"
     CDS             complement(10974..11780)
                     /gene="proC"
                     /locus_tag="LRB02_000727"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL4658954.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            11921..14494
                     /gene="mprF"
                     /locus_tag="LRB02_000728"
     CDS             11921..14494
                     /gene="mprF"
                     /locus_tag="LRB02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL4658955.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14636..15499
                     /locus_tag="LRB02_000729"
     CDS             14636..15499
                     /locus_tag="LRB02_000729"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL4658956.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            15744..16682
                     /locus_tag="LRB02_000730"
     CDS             15744..16682
                     /locus_tag="LRB02_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL4658957.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            16705..17385
                     /locus_tag="LRB02_000731"
     CDS             16705..17385
                     /locus_tag="LRB02_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4658958.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17358..18182
                     /locus_tag="LRB02_000732"
     CDS             17358..18182
                     /locus_tag="LRB02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4658959.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18349..19167
                     /gene="purR"
                     /locus_tag="LRB02_000733"
     CDS             18349..19167
                     /gene="purR"
                     /locus_tag="LRB02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL4658960.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19670..21043
                     /gene="glmU"
                     /locus_tag="LRB02_000734"
     CDS             19670..21043
                     /gene="glmU"
                     /locus_tag="LRB02_000734"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL4658961.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21137..22108
                     /locus_tag="LRB02_000735"
     CDS             21137..22108
                     /locus_tag="LRB02_000735"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL4658962.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22114..22216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22282..22851
                     /gene="thiT"
                     /locus_tag="LRB02_000736"
     CDS             22282..22851
                     /gene="thiT"
                     /locus_tag="LRB02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL4658963.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(22932..23705)
                     /locus_tag="LRB02_000737"
     CDS             complement(22932..23705)
                     /locus_tag="LRB02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658964.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            23971..25671
                     /locus_tag="LRB02_000738"
     CDS             23971..25671
                     /locus_tag="LRB02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL4658965.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25641..25859
                     /locus_tag="LRB02_000739"
     CDS             25641..25859
                     /locus_tag="LRB02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL4658966.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            25879..26769
                     /locus_tag="LRB02_000740"
     CDS             25879..26769
                     /locus_tag="LRB02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL4658967.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            26787..27071
                     /locus_tag="LRB02_000741"
     CDS             26787..27071
                     /locus_tag="LRB02_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4658968.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27558..28172
                     /gene="gmk"
                     /locus_tag="LRB02_000742"
     CDS             27558..28172
                     /gene="gmk"
                     /locus_tag="LRB02_000742"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL4658969.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28177..28482
                     /locus_tag="LRB02_000743"
     CDS             28177..28482
                     /locus_tag="LRB02_000743"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL4658970.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            28735..31143
                     /gene="priA"
                     /locus_tag="LRB02_000744"
     CDS             28735..31143
                     /gene="priA"
                     /locus_tag="LRB02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL4658971.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31158..31649
                     /gene="def"
                     /locus_tag="LRB02_000745"
     CDS             31158..31649
                     /gene="def"
                     /locus_tag="LRB02_000745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL4658972.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31642..32580
                     /locus_tag="LRB02_000746"
     CDS             31642..32580
                     /locus_tag="LRB02_000746"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL4658973.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32580..33938
                     /gene="rsmB"
                     /locus_tag="LRB02_000747"
     CDS             32580..33938
                     /gene="rsmB"
                     /locus_tag="LRB02_000747"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL4658974.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            33951..34691
                     /locus_tag="LRB02_000748"
     CDS             33951..34691
                     /locus_tag="LRB02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL4658975.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            34688..36757
                     /gene="pknB"
                     /locus_tag="LRB02_000749"
     CDS             34688..36757
                     /gene="pknB"
                     /locus_tag="LRB02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL4658976.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            36922..37821
                     /gene="rsgA"
                     /locus_tag="LRB02_000750"
     CDS             36922..37821
                     /gene="rsgA"
                     /locus_tag="LRB02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL4658977.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFDSENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            37834..38484
                     /locus_tag="LRB02_000751"
     CDS             37834..38484
                     /locus_tag="LRB02_000751"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL4658978.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            38485..39123
                     /locus_tag="LRB02_000752"
     CDS             38485..39123
                     /locus_tag="LRB02_000752"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL4658979.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            39278..40132
                     /gene="mreC"
                     /locus_tag="LRB02_000753"
     CDS             39278..40132
                     /gene="mreC"
                     /locus_tag="LRB02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL4658980.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            40136..40645
                     /gene="mreD"
                     /locus_tag="LRB02_000754"
     CDS             40136..40645
                     /gene="mreD"
                     /locus_tag="LRB02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL4658981.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT        13328 a         7201 c         8834 g        11286 t
ORIGIN      
        1 attcgtaagg ttcgtttgct tttttgtgtg cgaatcggta tgctaagaat aaaaaagcag
       61 ggggcgtttg cttagtggaa tatcaggtaa agaaatttga agaattgtca acagcagagt
      121 tttacgcgat cgtcaaagaa cgaatcgcag tttttgttgt ggaacaacag tgtccttatc
      181 aagaagttga tgacattgat ggacaagctt ggcatacgtt ttttcaagat actgatggga
      241 agatccttgc ctacacgcga gtatatgaag aagcggagag tatccatttt ggacgtgtcc
      301 ttgttcatca ggacaaccgt aaagatggtt taggaagaaa gattgttcaa gaaacgatta
      361 acatgatcca gaaaaatttt ccagaaaaac caatcgtgat cggcgcacaa gaatacctac
      421 aagcttttta tgagtcgttt ggattcaagg caatctcaga tgtgtatttg gaagatgata
      481 ttccacatat cgacatgaaa aaggaacaag tagcaagtta agaatctaaa aaaagaaagc
      541 cgacactgaa aatcggcttt ctttttttta gattctttat tatttagaag tatcggaaag
      601 atttgtttgc tctactggat gtgtattgtc ttctgccact tgtttggcaa gaagatcgcg
      661 tatgtcacta agataatctt ctgcagtagg agtttcttcc acttccactt gctcttcttt
      721 ggagcgtaaa ttcttcgcac ggtttgccgc tttgacaact aagaataaga caaaaccagt
      781 gatcagaaat gtaatgatag cactaataac gttaccaaac gcaaatgtga cgccttttgt
      841 cggcgaccag tcaagaattg aaagtgctcc ttctaaatca gaagtacctg tgatcattgc
      901 gacaaaccaa cctattagtg gtgtgatcaa gccttgcacc acttggttta cgatgcttgt
      961 aaacgcacta ccaataacaa cgccgacagc aagatccaag acatctccac gcatgatgaa
     1021 ctctttgaat tctttgatca tgaatgaccc tcccttcttt tatttgtctt tagtatatcg
     1081 aaaaataaaa aataaaaaaa caatatacgc ttttagccgt ctgtttcttt tgataaaaaa
     1141 catctatgct atactataaa gtgtaacatc atttagaaaa aacggcgaat tgcctgaact
     1201 tataaatttc aagcagagga ataagtaaaa aaggcaggtt gacccattaa aagagtaagc
     1261 gggcaatcaa ctgtgaaaag gagcatataa atatggtgta tcaattggca gaaggcgtga
     1321 atcttcatgt cttaccaaca aaacaataca aaacgatccg gatttttatc cgttttactg
     1381 cgcgtctgca acaagaagtc atcacgaaac gttcacttct cagcagcatg ttggaaacaa
     1441 atagtttgaa ttatccggat cagacaaagt taagtgcgca acttgcagag ctttatggcg
     1501 caagctttgg attatcagtt agaaaaaaag gaaatttaca ttggctaaac gcagggatca
     1561 gttttgtgaa tggtgaatat gtcaatgatc caaacctttt ttctcaagcg gtagattttt
     1621 taaaagaggt tttattttat ccaaatatta aaaatcatca gtttgatcag ttgacgtttg
     1681 acttggaaaa gaacaatctg cgtctatatt tggaaagttt gaaggaagat aaacaaactt
     1741 ttgcttccta tgccttgcaa gaactttatt ttgaaaacag cccggaacaa aagatcccag
     1801 ggttgggcgt agtcgaagat ttggacaaga tcactgctag ttcgctagcg tcctactatc
     1861 aagaaatgat ggcaaatgat caaatcgaca tttttgttgt cggcgatgtt gatccggaca
     1921 aagcagctga agcagttggg caattaccat ttgagtcgcg ggaaacagtt tatccagatc
     1981 tgttttatac ccaacctcaa gtgaatatcg tgaaggaacg acaagtacgt gagccaatcg
     2041 ttcaagcaaa gctgaaccta gcttatcaga caaatgtgta ttacgatgaa ccagaacgtt
     2101 ttgcgctaat ggtctttaac ggtttatttg gcggttttcc tcactctaaa cttttcatga
     2161 atgtcagaga gaaagaaagt ctagcttatt atgcctctag tagcgtggat acgttccgag
     2221 gattcatgag tgtccaaacc ggtatcgatg aaaagaaccg aaatcaagta cttcatctga
     2281 ttcacgagca attagaaagt ttgcgaaatg gtgaaataac agagttggaa ctgtctcaga
     2341 caaaagcaat gctgagaaac caatatctgc tttctctaga ctctccgcaa gcggcaatcg
     2401 aagcttcttt tttggacagc tggctgccgg aaacaaaatt atctgatgaa gaatggttga
     2461 aacgtatgga gagcgtaacg atcaaagaga tacaacaagt agctgagcaa atcgaattgc
     2521 aggcaatctt ctttttagca ggaggaaatg caaatgaata aaacagaata tgagcaaatc
     2581 aatgaaacac tttatcatga agtactgcca aacggtttga ccgtgtattt gttgcctaaa
     2641 aatgactatc acaagacata tggtttgttc agtacgaatt atggttcgat cgataacgaa
     2701 tttattccct atggtgaaaa agaaaaggtg aaagttccag atggaattgc ccatttttta
     2761 gaacataagt tgtttgaaaa agaagatggt gatgtctttc aattatttgg gaaacaagga
     2821 gcttccgcca atgctttcac aagttttacg aaaaccagct atctgttttc tacgacggat
     2881 caggtagaaa agaatctgac gactctgatc gattttgtac aagcacctta cttcacagaa
     2941 gaaactgtaa acaaggaaaa aggaatcatc ggtcaggaaa tccaaatgta tgaagacgac
     3001 cctaactggc ggatgttttt tggcatacta aataacttgt atcctacaca cccattgcat
     3061 atcgatattg cgggcacggt tgaaagtatc gacaaaatca cagctcaaga cttgtatacg
     3121 tgctatcgga cgttttatca gccaagcaat atggttttat ttgtagttgg taagatggag
     3181 cctgagaaat tgatgaaact tattcgggaa aaccaagaag caaaaaattt ccctccaaag
     3241 caagaaattg tacgttattt ccctgaaaac acaaaagaaa tcatcaaaca aagtgcattg
     3301 gaagcagcca ttacacgaga taaatttgta cttggtatta aaggattaga cacattgccg
     3361 caagaaggaa cagaactgtt acgctataaa acagcaatca atctgttgtt ccaaatgatt
     3421 ttaggcaata cttctcgaaa ctatctagcg atgtacaatc aaggaattat cgatgacagc
     3481 tttggttttg aattcagctt ggatcgtgaa tttcactttg cggacttcag cggagatacg
     3541 gacgaaccag agaaagcagc agaaaaagtc aaagaaatca ttcttggatt cgctgatgat
     3601 ccggaagtgt cagaaacaaa tctcgatttg ctgaagaaga aaatgcttgg acaatatttc
     3661 caatcattaa attcgatcga atatattgcc aatcaattta cgcaaagttt atttggcgat
     3721 cgaactttat ttgatcttcc tgaaatcatc gattcgatcc aaatgaaaga cgtactggca
     3781 gctggggaag catttataag tgaagaggcc ttcagcgagt tttacatgag accaaaataa
     3841 accagtattt tatgaggaaa agtcttaaat ttcgttttag acttgtagta ggaacctcct
     3901 ccaaaatcag cttttatggt attcttagtt gtgttggaaa aattagtgag aaaataaaac
     3961 aaatcatgct ctcttttaat caacttatat taaaataaaa tcaaacgaag taatcaaaga
     4021 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
     4081 aaatttttgt gatatttcgg cttattgccg aagaagctac ttctggaaca ccgtgtacca
     4141 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
     4201 cttaatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     4261 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacat acggaaaaat
     4321 atctccttat attttgccgg tttttgagct aataccgagg aacagctgat tgaacaccgt
     4381 ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca agttcaaaaa
     4441 acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc aggagaacaa
     4501 tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca aacataacgt
     4561 ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga tgaacgagtg
     4621 gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa aatatcttta
     4681 gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat cgacaatgat
     4741 gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca atacgctgaa
     4801 gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag caaattgaca
     4861 ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa agcaatgcat
     4921 caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt attaggattg
     4981 gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg tcaagctgat
     5041 ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga ggagtcctct
     5101 tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac aacaaaatca
     5161 agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc catcgcagta
     5221 aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa tcgtgtatgg
     5281 ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc aaatgaaaca
     5341 cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg tgcagcaagc
     5401 aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga aaacaatcag
     5461 agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa attagtttac
     5521 gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg tcgcggtcct
     5581 tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata aattcatttg
     5641 gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag ggaagaaagg
     5701 agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta tgatcccgat
     5761 tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag gagacaccac
     5821 actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta tcacagactg
     5881 gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca agtttgcgga
     5941 cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg accaaggaaa
     6001 ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca caggcttgcg
     6061 tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa aagtcaagac
     6121 ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat tcagcctgat
     6181 cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta tctattcagg
     6241 tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag tatcgtttag
     6301 tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt caaaaaacag
     6361 cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga gaacactgtt
     6421 tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct ctttttaaat
     6481 aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat gaaagcagaa
     6541 ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa tgccactttc
     6601 ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt tgtaggagat
     6661 aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga actcatactc
     6721 ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac tgcggctcac
     6781 ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc atattttgaa
     6841 acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat cgaaggcggg
     6901 gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac agatacacat
     6961 gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt ggaacaagta
     7021 cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa agttttgcgc
     7081 ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat tgagacacag
     7141 atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg actgacagtg
     7201 aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga aaaaatccaa
     7261 gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc aaaagtcgta
     7321 gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt gacagccggc
     7381 gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc aggcggattc
     7441 gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga gctattagca
     7501 gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc acgtgagata
     7561 gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac gttagaaggt
     7621 caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt ttcggaaaaa
     7681 tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca aggatttgat
     7741 cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg gttttttctt
     7801 gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg atttgtaaat
     7861 ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc tgcactgaaa
     7921 aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa aatcgaccaa
     7981 caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg tgtcggcggt
     8041 tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa aacaacagtt
     8101 gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt cattgatgct
     8161 gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga tgaactactt
     8221 ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt agtatcaagt
     8281 ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg agcagaaatc
     8341 gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc tcaagcattg
     8401 cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat caaccaaatc
     8461 cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg acgcgcattg
     8521 aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa acaaggtaca
     8581 gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc acctcctttc
     8641 aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg agaacttcta
     8701 gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc ttataaagaa
     8761 gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca tcctgaaatg
     8821 atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga agtcgctgtc
     8881 ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa attatgataa
     8941 acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt tttattgaat
     9001 aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa tatctgttca
     9061 tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct atatgaaact
     9121 agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt ttctttgttt
     9181 atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg ttttttgtac
     9241 acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga agctgtatag
     9301 atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac attctcctcg
     9361 ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag tcacgtcacg
     9421 agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt gcaaaaaaag
     9481 aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat cagaaatacc
     9541 gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct caagagaatc
     9601 gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa aaacgcgaga
     9661 actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt gaagagcgag
     9721 aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa attgctggtt
     9781 tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag ctgaatcatg
     9841 agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca gatcgcaaag
     9901 cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt tcagaaacga
     9961 ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt ggacgagaag
    10021 gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc gatgatacac
    10081 cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca cggatgacct
    10141 tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa atggtcgaga
    10201 aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca gcgtttgaag
    10261 tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg cgattcagaa
    10321 ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta gctggtatct
    10381 tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg ctccatgata
    10441 ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt gcggaattag
    10501 ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat catggagatg
    10561 tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc tcagcagcaa
    10621 gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga agtctggaaa
    10681 atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct ggacgcgaag
    10741 ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt ttagtacgtg
    10801 atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa gtaatcgtta
    10861 ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag aagagccgcg
    10921 gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta tcattatttt
    10981 ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat gattgctggg
    11041 atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc aggagaagaa
    11101 acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg acaagaaccc
    11161 ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt cagtacacca
    11221 gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc agcaaggcca
    11281 atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc ttcaaaaatc
    11341 atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat cgcagaaacg
    11401 cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc ctgaggatct
    11461 tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat gaataagact
    11521 ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg tttcacggct
    11581 aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc atggacgcca
    11641 gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa cacctcttct
    11701 ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat attccctgtg
    11761 ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct ttctttttct
    11821 atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa gataacattg
    11881 gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata aacgatactt
    11941 gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt tcggctccgt
    12001 gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt ggcaagatat
    12061 ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc tggctggact
    12121 gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct tagaaaaaca
    12181 aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa caaacacgat
    12241 caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta gtttttatgg
    12301 aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac tatttatgct
    12361 gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta ttcagtcaga
    12421 aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag caccagtcat
    12481 tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc caaaaggctt
    12541 gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg tatttttgat
    12601 catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta tgttcatgat
    12661 tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt ttgacgtttt
    12721 gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag tttggctatt
    12781 atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat tgttcatcac
    12841 acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt ttttccaaaa
    12901 ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga tggtacttct
    12961 atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat tgccgttctc
    13021 attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac tcggccttgc
    13081 tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt tactatcttt
    13141 tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg tatatgtctt
    13201 gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat tcgtttattc
    13261 ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg tttacgcaat
    13321 tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc attttatttt
    13381 gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct cattgctagc
    13441 tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca gtgagtacga
    13501 aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa gccatcagct
    13561 tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc aagtcgtttt
    13621 tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg gcaatcctaa
    13681 aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag gctatcagct
    13741 tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg gttttcactt
    13801 cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt ctcggctgag
    13861 tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt ggtacgatca
    13921 actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc ttcaaggtgc
    13981 acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat ctagcggtgt
    14041 cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc cgatgacgaa
    14101 gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg aattagctga
    14161 ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg aagccaatct
    14221 aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc aagaaaaagt
    14281 catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg cttatgaaaa
    14341 gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga aaggaagtaa
    14401 tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc
    14461 gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat
    14521 gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat
    14581 aagctatact tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg
    14641 tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt
    14701 atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc
    14761 agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc
    14821 atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa
    14881 atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg
    14941 attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa
    15001 tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc
    15061 ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt
    15121 accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg
    15181 tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc
    15241 tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga
    15301 agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta
    15361 cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca
    15421 ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt
    15481 agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc
    15541 taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca
    15601 tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat
    15661 tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt
    15721 tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag
    15781 gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt
    15841 tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg
    15901 aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg
    15961 caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg
    16021 aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag
    16081 cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag
    16141 aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta
    16201 taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa
    16261 cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact
    16321 ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt
    16381 atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg
    16441 aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag
    16501 tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag
    16561 gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag
    16621 gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt
    16681 aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac
    16741 tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc
    16801 attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta
    16861 ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta
    16921 agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg
    16981 atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa
    17041 aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat
    17101 cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt
    17161 gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc
    17221 agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta
    17281 atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg
    17341 agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg
    17401 ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac
    17461 aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct
    17521 ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat
    17581 tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg
    17641 ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa
    17701 gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt
    17761 taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg
    17821 ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga
    17881 tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac
    17941 ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct
    18001 tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa
    18061 ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt
    18121 tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact
    18181 aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt
    18241 ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa
    18301 acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt
    18361 cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca tgaattgatt
    18421 tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg
    18481 ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt
    18541 gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt
    18601 gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat
    18661 ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct
    18721 aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt
    18781 gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa
    18841 ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa
    18901 aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat
    18961 ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca
    19021 gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat
    19081 gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt
    19141 gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag
    19201 taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga
    19261 gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt
    19321 attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg
    19381 aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa
    19441 gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga
    19501 ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta
    19561 tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg
    19621 tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg
    19681 ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt
    19741 tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac
    19801 gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact
    19861 aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt
    19921 gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac
    19981 accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc
    20041 aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg
    20101 tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc
    20161 tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc
    20221 gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga
    20281 aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc
    20341 aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat
    20401 taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga
    20461 tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa
    20521 tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat
    20581 tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc
    20641 agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat
    20701 cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt
    20761 gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt
    20821 cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg
    20881 ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg
    20941 gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt
    21001 caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac
    21061 ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa
    21121 gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt
    21181 aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa
    21241 atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg
    21301 tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga
    21361 gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat
    21421 gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc
    21481 gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct
    21541 tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc
    21601 acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc
    21661 accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat
    21721 tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg
    21781 acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat
    21841 cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac
    21901 acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct
    21961 agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt
    22021 aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag
    22081 tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg
    22141 ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat
    22201 tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta
    22261 ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt
    22321 gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt
    22381 tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg
    22441 ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt
    22501 gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc
    22561 atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc
    22621 atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga
    22681 gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt ttctttcgta
    22741 atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc
    22801 caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag
    22861 aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat
    22921 aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag
    22981 tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg
    23041 ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg
    23101 atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct
    23161 ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt
    23221 acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca
    23281 tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta
    23341 ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat
    23401 aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc
    23461 cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt
    23521 tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc agaagcagat
    23581 ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca
    23641 gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca
    23701 gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta
    23761 aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca
    23821 ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga tgttttatga
    23881 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
    23941 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
    24001 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
    24061 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
    24121 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
    24181 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
    24241 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
    24301 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
    24361 aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg tgcttatcag
    24421 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
    24481 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
    24541 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
    24601 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
    24661 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
    24721 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
    24781 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
    24841 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
    24901 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
    24961 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
    25021 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
    25081 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
    25141 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
    25201 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
    25261 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
    25321 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
    25381 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
    25441 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
    25501 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
    25561 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
    25621 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
    25681 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
    25741 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
    25801 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
    25861 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
    25921 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
    25981 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
    26041 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
    26101 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
    26161 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
    26221 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
    26281 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
    26341 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
    26401 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
    26461 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
    26521 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
    26581 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
    26641 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
    26701 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
    26761 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
    26821 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
    26881 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
    26941 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
    27001 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
    27061 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
    27121 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
    27181 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt aagccaaatt
    27241 tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga aactaatcgg
    27301 cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata ataaccccaa
    27361 ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt gatgcaggac
    27421 agctttttta cgacctcttg gtacagggta gcttttttac caatttctag ctataattaa
    27481 agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta
    27541 gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct ggtccatccg
    27601 gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat gattttcagt
    27661 attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt
    27721 tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg ctggaatacg
    27781 cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag acattggatg
    27841 aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc aaagaaaaag
    27901 tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta aaatcacgga
    27961 tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa gttgcaaaag
    28021 aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa gtgccgctag
    28081 cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat cgcgtaatcg
    28141 gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc ttcaattgac
    28201 tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag taaacgcgcg
    28261 catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt aaaaagcgtc
    28321 ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca tcctgaatta
    28381 aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa
    28441 cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa aatagagcga
    28501 acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc acttattgtc
    28561 tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt atctgctgtt
    28621 aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta gtaaaatagg
    28681 caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca
    28741 ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc attcacttat
    28801 ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga agtccctttt
    28861 ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc agaagaatca
    28921 gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt ggtcaatgaa
    28981 gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa aatcacttgt
    29041 ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat ctatcctttg
    29101 gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc ctggaaggaa
    29161 gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca attagtagat
    29221 atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg
    29281 ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa
    29341 aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc ttatttcaaa
    29401 aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg gctggagttt
    29461 atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca aacgacggca
    29521 ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag
    29581 caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac agaagtatat
    29641 cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa
    29701 atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg
    29761 gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg
    29821 gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct gaaaaatatc
    29881 gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga gacaccgcgt
    29941 tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc aatagttcta
    30001 ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta
    30061 ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga gatcgtagat
    30121 atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt gcaggaaaaa
    30181 atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg tcgaggatat
    30241 tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa ttgcgatatc
    30301 tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg acatgaagaa
    30361 cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta tggaacaggt
    30421 actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat tttgcgtatg
    30481 gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa attcggtgca
    30541 aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga ctttccagaa
    30601 gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga ttttcgttct
    30661 agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg agcggaaaaa
    30721 gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga attggctaaa
    30781 aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg tggaggctat
    30841 cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca agtagcggca
    30901 aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca aagcttgcta
    30961 ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta tcaactaatc
    31021 atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt gaatggaagc
    31081 cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt aaactttatt
    31141 taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat aaattaaaaa
    31201 gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta gacaatttat
    31261 atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta ggacaaaaca
    31321 agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt aacccagaaa
    31381 tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt ccgcatgtct
    31441 atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga gacggagaag
    31501 agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa atcgatcact
    31561 tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa gaacttgaag
    31621 aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc tgctttttct
    31681 gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt ggtaacacag
    31741 cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa agaagcggct
    31801 ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc agaaatggaa
    31861 gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca attcttgcct
    31921 gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc tttacttcct
    31981 aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa agaaactggt
    32041 gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc
    32101 ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag ccttttagga
    32161 agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc agtacctcaa
    32221 gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc aatcgattgg
    32281 cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca
    32341 ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt ggcagaaact
    32401 acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg gattgcttgc
    32461 ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg aaaacaggcc
    32521 gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca ggtgaaataa
    32581 tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta ctgacattag
    32641 agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg atcaaacaaa
    32701 gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt acgattagcc
    32761 atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa aaagtggatg
    32821 actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta gataaagtcc
    32881 ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga
    32941 cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca ccagatctgc
    33001 aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct ctatggatga
    33061 ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt ttatcgctat
    33121 atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga gatcaggcca
    33181 tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt ccatacggta
    33241 tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc gtcatgacga
    33301 tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct gaacatcatg
    33361 tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct tttttagatc
    33421 cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc aaactcattg
    33481 aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta
    33541 gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta gacgcgcctt
    33601 gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa cagccggatg
    33661 aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca ccaactttaa
    33721 agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa aaccaagacg
    33781 ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt ttgaccaatc
    33841 ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat cagttttaca
    33901 cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg
    33961 aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc agtgtattta
    34021 ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt catcgtgccg
    34081 gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa aaacaagaag
    34141 ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa gaaaatatgc
    34201 tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca acaatcgtag
    34261 cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc agagcttatt
    34321 tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc aatgaactag
    34381 tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa aatattttaa
    34441 cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat ctttggcaac
    34501 taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct gaagaagaaa
    34561 taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag ctgattgatc
    34621 gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa tataaggagg
    34681 acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat
    34741 cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt
    34801 agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc gacgatttca
    34861 gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg tttacgatgt
    34921 tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa tggacttgaa
    34981 acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata ttatgcagca
    35041 gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag atttgaaacc
    35101 ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg ggattgctat
    35161 cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg ttcattatct
    35221 gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt atgctttagg
    35281 gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac
    35341 aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg atccaaatgt
    35401 gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg cagaccgtta
    35461 taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa
    35521 cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca caccccttac
    35581 ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag acaagccaga
    35641 aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga aaggacagaa
    35701 aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa cagctgtatt
    35761 ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc tttctgaagc
    35821 ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa cagaagaaat
    35881 tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag cgggaacgac
    35941 agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa aaatcaaatt
    36001 agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag aattagggtt
    36061 ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa gcggaatgat
    36121 catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca cgattacttt
    36181 cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag aaaactacga
    36241 aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta gtgctactta
    36301 tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag cctcgggtga
    36361 agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag agcctaaatt
    36421 gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta gtgtcggtgc
    36481 agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag ataaagtgat
    36541 tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga acgtaattta
    36601 ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag acagttctag
    36661 cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac
    36721 aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa tatatcagaa
    36781 gggaatgcga cattgttttg ttgcattctc ttttttgtgt ttctatctct tttgatcaat
    36841 tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc tcttcgtatg
    36901 gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa agcaatcagc
    36961 ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag aggaaatttc
    37021 cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgatag tgaaaatcaa
    37081 acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg cccaccagtc
    37141 acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt ttcttataat
    37201 ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat tatttttttg
    37261 acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca aacctatact
    37321 aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga actgatccgt
    37381 tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta
    37441 ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga tgcacttgga
    37501 agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg gttagtagca
    37561 gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga actgccgaga
    37621 caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg catgcatgct
    37681 aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc acagacacgt
    37741 tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa
    37801 aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc cattttgagt
    37861 gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg cgcagattac
    37921 atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg gccaaatatt
    37981 gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat gatcgttgat
    38041 ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac tgtccacgtc
    38101 gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg agttaaagct
    38161 ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt gcctttggct
    38221 gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt tattgaagaa
    38281 acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg gacttatgca
    38341 atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg
    38401 gatgtcttcg ttgcagggtc atatatctat ggagccgaaa atccagtcgg acaaatcgaa
    38461 cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg taatccgcaa
    38521 aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga ccgtggtgcg
    38581 ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt tgattcattg
    38641 tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg ttcccctgca
    38701 gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg ttacccgcaa
    38761 gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt agccaatctc
    38821 tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat cagagattgt
    38881 caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat accagatatg
    38941 aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa aggcagcaag
    39001 tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa cgaatttctt
    39061 tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca aagcaaagat
    39121 tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga atctattatt
    39181 tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt tagtgataaa
    39241 attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca atcctaataa
    39301 aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa gtgtgaccgc
    39361 tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta atgacagtgt
    39421 tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg gagtagattc
    39481 tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag
    39541 ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa
    39601 agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa atgtcattac
    39661 tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta aagacggtat
    39721 cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa
    39781 tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca atcattttcc
    39841 agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact atgatgaaaa
    39901 gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag aaggggatgt
    39961 tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg gtacggttat
    40021 taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac cttatgcaga
    40081 aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt agtccatgtt
    40141 gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga tgttgataga
    40201 tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt caaatgccca
    40261 ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt atttactgat
    40321 taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta tcggtatcta
    40381 tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg ttatccatgt
    40441 caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg aattgtttac
    40501 gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt ttattacaag
    40561 gtttttagga cctacactgc tgttgaacat gattatattt gtattattca tttttccctt
    40621 taagaaatta ttcagtgatg aataatttc
//
LOCUS       DAFFQY010000016        40040 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFFQY010000016 DAFFQY010000000
VERSION     DAFFQY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.016"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            117..971
                     /locus_tag="LRB02_000755"
     CDS             117..971
                     /locus_tag="LRB02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL4658982.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="LRB02_000756"
     CDS             972..2309
                     /locus_tag="LRB02_000756"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL4658983.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="LRB02_000757"
     CDS             2314..2538
                     /locus_tag="LRB02_000757"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL4658984.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="LRB02_000758"
     CDS             2540..3430
                     /locus_tag="LRB02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL4658985.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="LRB02_000759"
     CDS             3439..4257
                     /locus_tag="LRB02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL4658986.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="LRB02_000760"
     CDS             4364..4813
                     /locus_tag="LRB02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL4658987.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="LRB02_000761"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="LRB02_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL4658988.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="LRB02_000762"
     CDS             complement(6568..6684)
                     /locus_tag="LRB02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL4658989.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="LRB02_000763"
     CDS             complement(6757..7713)
                     /locus_tag="LRB02_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4658990.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="LRB02_000764"
     CDS             7884..8252
                     /locus_tag="LRB02_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL4658991.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="LRB02_000765"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="LRB02_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL4658992.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="LRB02_000766"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="LRB02_000766"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL4658993.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="LRB02_000767"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="LRB02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL4658994.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="LRB02_000768"
     CDS             10240..12432
                     /locus_tag="LRB02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL4658995.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="LRB02_000769"
     CDS             12458..13420
                     /locus_tag="LRB02_000769"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL4658996.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="LRB02_000770"
     CDS             13434..14795
                     /locus_tag="LRB02_000770"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL4658997.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="LRB02_000771"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="LRB02_000771"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL4658998.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="LRB02_000772"
     CDS             15924..17144
                     /locus_tag="LRB02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL4658999.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="LRB02_000773"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="LRB02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL4659000.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="LRB02_000774"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="LRB02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL4659001.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="LRB02_000775"
     CDS             19906..20583
                     /locus_tag="LRB02_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL4659002.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="LRB02_000776"
     CDS             20597..21196
                     /locus_tag="LRB02_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL4659003.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="LRB02_000777"
     CDS             21211..21498
                     /locus_tag="LRB02_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL4659004.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="LRB02_000778"
     CDS             21547..22329
                     /locus_tag="LRB02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL4659005.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="LRB02_000779"
     CDS             22350..23057
                     /locus_tag="LRB02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL4659006.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="LRB02_000780"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="LRB02_000780"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL4659007.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="LRB02_000781"
     CDS             complement(26166..27692)
                     /locus_tag="LRB02_000781"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL4659008.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGNYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="LRB02_000782"
     CDS             complement(27707..28354)
                     /locus_tag="LRB02_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL4659009.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="LRB02_000783"
     CDS             complement(28538..28867)
                     /locus_tag="LRB02_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL4659010.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="LRB02_000784"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="LRB02_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL4659011.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="LRB02_000785"
     CDS             29788..30801
                     /locus_tag="LRB02_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4659012.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="LRB02_000786"
     CDS             30801..32078
                     /locus_tag="LRB02_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4659013.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="LRB02_000787"
     CDS             32141..34843
                     /locus_tag="LRB02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4659014.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="LRB02_000788"
     CDS             34995..35312
                     /locus_tag="LRB02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4659015.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="LRB02_000789"
     CDS             35342..35662
                     /locus_tag="LRB02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4659016.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="LRB02_000790"
     CDS             35748..37208
                     /locus_tag="LRB02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659017.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="LRB02_000791"
     CDS             37276..37497
                     /locus_tag="LRB02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659018.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="LRB02_000792"
     CDS             37528..37710
                     /locus_tag="LRB02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL4659019.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="LRB02_000793"
     CDS             37710..38123
                     /locus_tag="LRB02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL4659020.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="LRB02_000794"
     CDS             38246..39427
                     /locus_tag="LRB02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659021.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39498..39662)
                     /locus_tag="LRB02_000795"
     CDS             complement(39498..39662)
                     /locus_tag="LRB02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659022.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39816..39889
                     /locus_tag="LRB02_000796"
     tRNA            39816..39889
                     /locus_tag="LRB02_000796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
BASE COUNT        13389 a         6826 c         8590 g        11235 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaatttat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtttcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa
//
LOCUS       DAFFQY010000017        39957 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFFQY010000017 DAFFQY010000000
VERSION     DAFFQY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.017"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LRB02_000797"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LRB02_000797"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL4659023.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LRB02_000798"
     CDS             1011..2258
                     /locus_tag="LRB02_000798"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL4659024.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2677
                     /locus_tag="LRB02_000799"
                     /pseudo
     CDS             2334..2677
                     /locus_tag="LRB02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2794..3120)
                     /locus_tag="LRB02_000800"
     CDS             complement(2794..3120)
                     /locus_tag="LRB02_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659025.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3493..4695)
                     /locus_tag="LRB02_000801"
     CDS             complement(3493..4695)
                     /locus_tag="LRB02_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4659026.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4812..5327
                     /locus_tag="LRB02_000802"
     CDS             4812..5327
                     /locus_tag="LRB02_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4659027.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="LRB02_000803"
     CDS             complement(5526..5972)
                     /locus_tag="LRB02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL4659028.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="LRB02_000804"
     CDS             complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="LRB02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL4659029.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="LRB02_000805"
     CDS             complement(7851..8480)
                     /locus_tag="LRB02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4659030.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="LRB02_000806"
     CDS             complement(8464..8928)
                     /locus_tag="LRB02_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4659031.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="LRB02_000807"
     CDS             complement(9415..10683)
                     /locus_tag="LRB02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL4659032.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="LRB02_000808"
     CDS             complement(10914..11651)
                     /locus_tag="LRB02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4659033.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="LRB02_000809"
     CDS             complement(11718..12287)
                     /locus_tag="LRB02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL4659034.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="LRB02_000810"
     CDS             12415..13287
                     /locus_tag="LRB02_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4659035.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="LRB02_000811"
     CDS             13629..14501
                     /locus_tag="LRB02_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4659036.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="LRB02_000812"
     CDS             14498..16456
                     /locus_tag="LRB02_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4659037.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="LRB02_000813"
     CDS             complement(16707..17558)
                     /locus_tag="LRB02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4659038.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="LRB02_000814"
     CDS             17681..18148
                     /locus_tag="LRB02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL4659039.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="LRB02_000815"
     CDS             complement(18411..18893)
                     /locus_tag="LRB02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL4659040.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="LRB02_000816"
     CDS             complement(18993..19856)
                     /locus_tag="LRB02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL4659041.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="LRB02_000817"
     CDS             20454..21302
                     /locus_tag="LRB02_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659042.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="LRB02_000818"
     CDS             complement(21473..21814)
                     /locus_tag="LRB02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL4659043.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="LRB02_000819"
     CDS             21900..22607
                     /locus_tag="LRB02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL4659044.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="LRB02_000820"
     CDS             22594..22917
                     /locus_tag="LRB02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL4659045.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="LRB02_000821"
     CDS             complement(23006..24043)
                     /locus_tag="LRB02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4659046.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="LRB02_000822"
     CDS             complement(24490..25473)
                     /locus_tag="LRB02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL4659047.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="LRB02_000823"
     CDS             complement(25889..26659)
                     /locus_tag="LRB02_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659048.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="LRB02_000824"
     CDS             complement(26652..27587)
                     /locus_tag="LRB02_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659049.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="LRB02_000825"
     CDS             complement(28181..29830)
                     /locus_tag="LRB02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL4659050.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="LRB02_000826"
     CDS             complement(30025..30921)
                     /locus_tag="LRB02_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4659051.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="LRB02_000827"
     CDS             complement(30914..31594)
                     /locus_tag="LRB02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659052.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="LRB02_000828"
     CDS             complement(31595..32377)
                     /locus_tag="LRB02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659053.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="LRB02_000829"
     CDS             complement(32370..33287)
                     /locus_tag="LRB02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659054.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="LRB02_000830"
     CDS             complement(33290..33574)
                     /locus_tag="LRB02_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659055.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34362..37589
                     /gene="fss3"
                     /locus_tag="LRB02_000831"
     CDS             34362..37589
                     /gene="fss3"
                     /locus_tag="LRB02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL4659056.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37702..38016
                     /locus_tag="LRB02_000832"
     CDS             37702..38016
                     /locus_tag="LRB02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4659057.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38029..38403
                     /locus_tag="LRB02_000833"
     CDS             38029..38403
                     /locus_tag="LRB02_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4659058.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38413..38748
                     /locus_tag="LRB02_000834"
     CDS             38413..38748
                     /locus_tag="LRB02_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL4659059.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38831..>39957
                     /locus_tag="LRB02_000835"
     CDS             38831..>39957
                     /locus_tag="LRB02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL4659060.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12155 a         8142 c         7384 g        12276 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gattttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
     2401 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa
     2461 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
     2521 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
     2581 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
     2641 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
     2701 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
     2761 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
     2821 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
     2881 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
     2941 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
     3001 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
     3061 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
     3121 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
     3181 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
     3241 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
     3301 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
     3361 tcttggtaaa cggcgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3421 tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca
     3481 acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat aaatgataca
     3541 acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt caccaatatg
     3601 ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc ggacaaaact
     3661 atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa tcgtcgtcaa
     3721 cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg gtacaaggac
     3781 tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga caaatgctgc
     3841 taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga aaatcgaagc
     3901 aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt cagaatagcc
     3961 aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat ataacaatgc
     4021 gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt cccaaaggcc
     4081 tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta taaaaccgca
     4141 aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc ccaagacccc
     4201 accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca tcatgattgc
     4261 tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag agacaaataa
     4321 agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat tcccagataa
     4381 tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg tttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 atactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccaca cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 agatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttttttctt cgtatcattg aataagtttg aaaaaccttg atttataaag gttttcccgt
    34141 aaatatattt tattatattt gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa
    34201 tttaaaggca gtatatttcg tattttagat taaattttta ggctcattca ttctcaaatc
    34261 cattctattc agtcaataaa aattttaaaa agactttata aacaaagatg ttcaagcgag
    34321 catcttattt ttttgtgctt aatttgaaag gagaacattt catgtataaa aaacaaaaaa
    34381 agaaattatg tgtattaact attttgagtt tggctgttca agcaattgtt ggagcaattg
    34441 ctcctacgat tacttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag
    34501 atttagcaca tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag
    34561 tcactgttcc gcattatgca gtgtacaacg gctttaaaca agatattttc tgtattgaac
    34621 caggagtccc gatttacaat gagttcactc ctggctatga gaaaaacccg ttaccagata
    34681 tgtccgataa agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata
    34741 ctcatattgt ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa
    34801 taaaacgatc agatggtagt gccgtaaata tcgcagccat tgaggcaaaa ataaatcaag
    34861 ccattgacga ttatcagaag aaaccaacct tccataatag tacggctaaa acagtattag
    34921 ggcaatcgac tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg
    34981 tggaaaatgc cgcaaacatt gattatcgtg tgaatggtaa tcagttgata atcaccccaa
    35041 atgccaattc aaaagaaagt ggtgtgctga cgcttaagaa atctgctggt accggaacac
    35101 ctgtcgccta taaaaaggct ggccaacaga ctttaatggc cggggcgatt gataaaccta
    35161 atacctacac aatcaaaatt gatgtggaaa cggagggttc tttaaagatc aaaaaagtcg
    35221 ataaagaatc gggtgatgtt gtaccaggaa cggttttcca tttagacttt ggaaaaactt
    35281 tacctgcaaa agacgtgact actgacaaag aaggcattgc tacattggat gagattcctc
    35341 atggtacaaa agtaaccatt accgaaaaat cagtaccggc gccttatacg attgatacca
    35401 cccccatgac taccaccatt aaagcaggcg aaaccattta cgtgacttca aaaaacgcac
    35461 gggaaaaagg gcaaatcatt ctggataaaa gcggagttga aacagggagt gatctttgga
    35521 atgacaatta ttccttagct ggtaatacgt ttgccattcg caaagacagt ccaaccggtg
    35581 aaatcgttca agaaatgacc acggatgaaa acggtcatgc ggaaacacca aaagagattg
    35641 ctaatgcgtt ggaattggga acctattatg tgaccgaaac taaagctagt catgggttcg
    35701 tgaatacgtt caaacctgta aaagtcgaat tgaagtatgc caatcaaacc gtagcccttg
    35761 ttaccagtaa cgtaaaaggg caaaatcaag aagtcactgg cgaaaccact ttgacgaaag
    35821 aagacaaaga tactggggat aaagcacaag gtaaggcagt ctttgagggt gctgaataca
    35881 ccttgtttac tgctaaggat ggaaaagctg ttaaatggag tgaggctttt aaaccagagt
    35941 tagcgaaggg aacgaaaggt tctgatgaaa cggtcacttt gactttagac gaaaagaacc
    36001 aagttgccgt taaacattta gctatcaacg agtattattg gcaagaaaca aaagcgcccg
    36061 aaggatatag ccttgatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg
    36121 aaaaaaatgc cgtgattacc cgaaatgtta cggcaaaaga acaagtgatt cgctttggct
    36181 ttgatttctt taaatttgct ggatcagctg cgggcactac cgaaactggc ttcaatgatc
    36241 tgacctttaa agtgtcgcca ttggaaggga ccaatgaaat cacaggtgcc gaagatgaag
    36301 cgaccacagc ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc
    36361 cttatgggga ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc
    36421 cattagaaat ccgttctact tttaaggaaa acaaagaaga ctttgtgaag agtgagtatg
    36481 tctttaccat taccgaacaa gaccaaaaac aaccaattaa aacggtgacc gttccttacg
    36541 agaagctgac gaacaaagca ttttctgtta gtttgaaccg tttaatgctc tatgatttgc
    36601 ctgaggaaga agatagttta acttctcttg cgacttggaa agacggaaac aaagaattaa
    36661 cttctctgga ttctaccgag ctagttgata aattgagtta caatctacat gaaatcaaag
    36721 aagactggta tgtggtagcg caagccattg atgtggatgc gacaaaagct gcccaagaaa
    36781 aagatgaaaa agccaaacca gtggtgattg cagaaacaag cgcaacgttg gcgaacaaag
    36841 agaaaacagg aacttggaaa attcagcata aattaaccgc cgaacaagtt ttaaataaaa
    36901 ctattgtctt attcaactat gtgtatgaaa ataaggaagc ttttgaagca ggcgataagc
    36961 cagtagcgaa ggatgttagc ttgaataacc aagcccaaac tgtcagttgt acagtggaac
    37021 accatgtttc tatccaaaca aaggcccacc tagaaaacgg ttctcagacc tttactcatg
    37081 gtgatgtggt ggatatgttt gatgatgtgt cgattactca tgatgtactg gatggctcaa
    37141 aagaagcttt cgaaacaatt ctgtatgctt tattaccgga tggtacaaac aaagaaattt
    37201 ggaaatctgg caagattgat tatgaagtaa atgataaaga attcaccaaa actgtgcttg
    37261 cgaaaaaagt agataccgga aaatatccag aaggaaccaa gtttactttt gcggaaatca
    37321 attacgataa agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc
    37381 aaaccttaac accaaaagaa gttccaacca tactaagtac gccgaaacaa ccggaaacac
    37441 caactgttcc aagtggctct caagaatcta gtcccacagt gaagaccttc cctcaaaccg
    37501 gggagaaaaa ttccaatgtt ttactgttca ttggttttac tttgattttt gcgacggcgg
    37561 gctattattt ctggaatcgc cggaactaag gtgatgagat gatacaagta aatcctaaga
    37621 accaacgtgc gcgaccgcct cctaaaaaag tagtcgcgct atttttgtat ccaaaactaa
    37681 caatgaaaag aggaaaagaa gatggaattg aaatttgtcg tgccagatat ggctgaaacc
    37741 tttggaaagt taagttatgc cggtgaagga gaaattataa cagaaggata tggacggaat
    37801 actacggtga ttggtcgcag ttatcatttg tattccagta agcaacgggc cgatgatatt
    37861 gaggtggtag tagctgcgga agccggtgaa aaggattttg atcaagacca accgctaaaa
    37921 gccgtgaatc cccatttggt tgccaaaggc tatgagattg agaatcgtgg gtttactgat
    37981 tacgtgttgt atgttgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca
    38041 gaaggtattg tcgtagattc aggtttaaca tttgggaagt tgcgtttttc cgcattacgc
    38101 cgagaagtac ggaagcaaaa tgaggatgga acaattggta atgaggtaaa agaacgaacc
    38161 tataatttaa aatcctctgc acaagggcga atgattcaag ttagtatccc agctaatgta
    38221 cccttacgcg agtttgctta tgatgcagag gtagagttgg tcaatccaat cgtggatacc
    38281 gttgccaatt atgtttttcg agaaggaact accgttaatt ggttcattaa ggcggatgat
    38341 ttagtcttga agcggcagcc gaatcaagga aatcctacga accaaaatga aagaaagaaa
    38401 taggtgaaga ttatggaaat taatgttgaa catattttag attgtctcga tcaatatgga
    38461 aaaggcgagc tgactgagga acaactcgtt aaagcattaa cctctgatga aaaaatgttt
    38521 ctgattatac atcaaggttt acttggagaa aatagcgaca gtgaagaaga ctctgatgta
    38581 ctagactggt taggagaaga agagtctttc tttatagttg tcgaagtaaa tgaaaactta
    38641 tgtcggcagg cagaatccat tttggaagaa attggtgtgg agatgccgga tgctattgaa
    38701 ggctttttaa atcaattggt tgaaacgaaa caactcccct cctcgtagca gtaaatgact
    38761 agcataactt tttgaaattg cctaatgaaa tcctattggg ctttttcgat tggaggtgga
    38821 aaccatcaaa atgtacaaag gacatcgcat acgcgcagga gatcagcact tggtttatca
    38881 ctttgttctt ggctggctac tcgctctgtt catcggttgg atgagcgtct tttattttca
    38941 agagtttaga caatttgata tttctaagct atcactttct actattgaaa tcgtttggtc
    39001 tataaaagat ttagtatgtc tgcttggaag tctcgctttt tcaggagcta tgatattgct
    39061 ttatattcac ttttttcttg atcattggcg aagcttgtgg catcgacaaa agctagctcg
    39121 gatgattcta gagaaccatt ggtatgaagt gaaacaaact caaagtgaag gctttttcaa
    39181 agatttgaat agtagccgaa ccagagagac tatcagttac ttccccaaaa tctattatcg
    39241 aatgaaagat ggattactgt ctattcgcgt tcaaatttca cttgggaaat accaagatca
    39301 gcttttaaaa ttggaaaaga agttagaaag cggactgtat tgtgagttag tagaaaagga
    39361 actcaaagac tcctatgtgg agtatacttt actgtatgat atgatcgcca atcgaattgg
    39421 gatagacgaa gtagtggcag aaaatgggac tctgcgattg atgaaaaatc aagtatgggc
    39481 ttatgattct ttaccccata tgttaatcgc tggtggcacg ggtggaggga agacctattt
    39541 cctcctcacc attatcgaag cactattgaa gtcagatgca gaattgttta tcctcgatcc
    39601 caaaaatgca gatttagctg atttaggtac ggtgatgcct catgtttatt cacaaaagga
    39661 agaaatttct gcttgtgtgg aagatttcta tgaacgtatg atggctcgta gcaaggcaat
    39721 gaaagaaatg tctaactaca aaacaggaga gaattatgcc tatcttggac ttccacccaa
    39781 ctttttaatc tttgatgaat acgtggctta tatggagatg ttaacgacga aagaaagtgc
    39841 ggtgattttg aataagctca aacaaatcgt gatgttgggt cgtcaatctg gtttctttct
    39901 gattttggct tgccaaaggc cggatgcgaa atatttagga gacggaatcc gcgatca
//
LOCUS       DAFFQY010000018        38333 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFFQY010000018 DAFFQY010000000
VERSION     DAFFQY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.018"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            161..391
                     /locus_tag="LRB02_000836"
     CDS             161..391
                     /locus_tag="LRB02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL4659061.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="LRB02_000837"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="LRB02_000837"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL4659062.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="LRB02_000838"
     CDS             1802..1978
                     /locus_tag="LRB02_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL4659063.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="LRB02_000839"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="LRB02_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL4659064.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="LRB02_000840"
     CDS             2593..3060
                     /locus_tag="LRB02_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659065.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="LRB02_000841"
     CDS             3145..4029
                     /locus_tag="LRB02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4659066.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="LRB02_000842"
     CDS             4143..4379
                     /locus_tag="LRB02_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL4659067.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="LRB02_000843"
     tRNA            4467..4547
                     /locus_tag="LRB02_000843"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="LRB02_000844"
     tRNA            4554..4625
                     /locus_tag="LRB02_000844"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="LRB02_000845"
     CDS             4750..4920
                     /locus_tag="LRB02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659068.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="LRB02_000846"
     CDS             5140..5847
                     /locus_tag="LRB02_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4659069.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="LRB02_000847"
     CDS             complement(5907..6551)
                     /locus_tag="LRB02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL4659070.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="LRB02_000848"
     CDS             6677..6964
                     /locus_tag="LRB02_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659071.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="LRB02_000849"
     CDS             7072..7392
                     /locus_tag="LRB02_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4659072.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="LRB02_000850"
     CDS             7473..8786
                     /locus_tag="LRB02_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL4659073.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="LRB02_000851"
     CDS             8783..9502
                     /locus_tag="LRB02_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL4659074.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="LRB02_000852"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="LRB02_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL4659075.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="LRB02_000853"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="LRB02_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL4659076.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13063..14164
                     /gene="prfB"
                     /locus_tag="LRB02_000854"
     CDS             join(13063..13134,13136..14164)
                     /gene="prfB"
                     /locus_tag="LRB02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL4659077.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14269..14955
                     /gene="ftsE"
                     /locus_tag="LRB02_000855"
     CDS             14269..14955
                     /gene="ftsE"
                     /locus_tag="LRB02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL4659078.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14939..15832
                     /locus_tag="LRB02_000856"
     CDS             14939..15832
                     /locus_tag="LRB02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659079.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15984..17432
                     /gene="cls"
                     /locus_tag="LRB02_000857"
     CDS             15984..17432
                     /gene="cls"
                     /locus_tag="LRB02_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL4659080.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17769..18626
                     /locus_tag="LRB02_000858"
     CDS             17769..18626
                     /locus_tag="LRB02_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL4659081.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18741..19661
                     /gene="pstC"
                     /locus_tag="LRB02_000859"
     CDS             18741..19661
                     /gene="pstC"
                     /locus_tag="LRB02_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL4659082.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19661..20545
                     /gene="pstA"
                     /locus_tag="LRB02_000860"
     CDS             19661..20545
                     /gene="pstA"
                     /locus_tag="LRB02_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL4659083.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20560..21366
                     /gene="pstB"
                     /locus_tag="LRB02_000861"
     CDS             20560..21366
                     /gene="pstB"
                     /locus_tag="LRB02_000861"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL4659084.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21382..22140
                     /locus_tag="LRB02_000862"
     CDS             21382..22140
                     /locus_tag="LRB02_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL4659085.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22153..22830
                     /gene="phoU"
                     /locus_tag="LRB02_000863"
     CDS             22153..22830
                     /gene="phoU"
                     /locus_tag="LRB02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL4659086.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23068..24099
                     /locus_tag="LRB02_000864"
     CDS             23068..24099
                     /locus_tag="LRB02_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL4659087.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24096..24863
                     /locus_tag="LRB02_000865"
     CDS             24096..24863
                     /locus_tag="LRB02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL4659088.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24963..26531
                     /gene="liaX"
                     /locus_tag="LRB02_000866"
     CDS             24963..26531
                     /gene="liaX"
                     /locus_tag="LRB02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL4659089.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26828..27148
                     /locus_tag="LRB02_000867"
     CDS             26828..27148
                     /locus_tag="LRB02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL4659090.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27148..27498
                     /locus_tag="LRB02_000868"
     CDS             27148..27498
                     /locus_tag="LRB02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL4659091.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27665..28603
                     /locus_tag="LRB02_000869"
     CDS             27665..28603
                     /locus_tag="LRB02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL4659092.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28618..29448
                     /locus_tag="LRB02_000870"
     CDS             28618..29448
                     /locus_tag="LRB02_000870"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL4659093.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29492..30517
                     /locus_tag="LRB02_000871"
     CDS             29492..30517
                     /locus_tag="LRB02_000871"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL4659094.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30532..31470
                     /gene="galU"
                     /locus_tag="LRB02_000872"
     CDS             30532..31470
                     /gene="galU"
                     /locus_tag="LRB02_000872"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL4659095.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31595..32035
                     /locus_tag="LRB02_000873"
     CDS             31595..32035
                     /locus_tag="LRB02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL4659096.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32038..32640
                     /locus_tag="LRB02_000874"
     CDS             32038..32640
                     /locus_tag="LRB02_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL4659097.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32703..33806)
                     /locus_tag="LRB02_000875"
     CDS             complement(32703..33806)
                     /locus_tag="LRB02_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL4659098.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="LRB02_000876"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="LRB02_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL4659099.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35372..35767
                     /locus_tag="LRB02_000877"
     CDS             35372..35767
                     /locus_tag="LRB02_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659100.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTCSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35767..36018
                     /locus_tag="LRB02_000878"
     CDS             35767..36018
                     /locus_tag="LRB02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659101.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36061..36456
                     /locus_tag="LRB02_000879"
     CDS             36061..36456
                     /locus_tag="LRB02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659102.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36453..37709
                     /locus_tag="LRB02_000880"
     CDS             36453..37709
                     /locus_tag="LRB02_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL4659103.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37721..38044
                     /locus_tag="LRB02_000881"
     CDS             37721..38044
                     /locus_tag="LRB02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659104.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38037..38303
                     /locus_tag="LRB02_000882"
     CDS             38037..38303
                     /locus_tag="LRB02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659105.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12858 a         6514 c         8260 g        10701 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aggttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tcttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttggggttat atccacacca cgtacaattg atgggtggaa tcgtgcttca
    10681 cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc
    10741 agtatatcta aatgctctga ctggcgaagg ggtacacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga actttataat ttcttaggat taacagtggg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gtgatatcac
    10921 ttatagtacg aataatgaat taggatttga ctacttgaga gacaatatgg tcgtttaccg
    10981 ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcaatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tgtacaatca aaaacaatca gtttaaccga agcagggatc gaaaaagcag aagaaacatt
    11221 cggtttggat aacttatacg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactatatca tgcttttaga tatcgattat gttgtccaag acggaaaagt
    11341 actgattgtt gaccagttca ctggtcggat catggatgga cgccgttatt cagatggact
    11401 tcaccaagca atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaaccatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggaa tgactggtac
    11521 agctaaaaca gaagaagaag aattcagaga aatttataac atccaagtat accagattcc
    11581 gacaaatcgt ccggttatcc gtgatgatcg tccagatttg ctttacccaa cattacaaag
    11641 caagttccac gctgtcgtgc aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt
    11701 agtcggaact gttgcggttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtgctga atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa
    11821 tgctggtcaa aaaggtgctg tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagttagga cttggggtaa gagaacttgg tggtctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacag ttctacttgt cattggaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaatct ttcttagatc ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa
    12121 gatgctgacg aaacaagtag aatctgcaca aaaacgtgta gaaggaaata actatgacac
    12181 acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa caacgtgaag taatctataa
    12241 acagcgacaa gaagtaatca tggaagaaaa aagcttatcc aagccactaa tgaacatggt
    12301 caaacggaca atcagtcgtg tagtagatgc gcatacacaa ttagagaaag aaaattggaa
    12361 tttggaaagt atcatcgatt ttgccggcag tactcttgtc catgaagata gtattactct
    12421 tgcagatatc gaaggaaaaa cagctcagga gatcaaagag tatctaaacg aacgagcaca
    12481 agaagtattt aataccaaag cggctcaact aaatggtcca gagcaactgt tggaatttga
    12541 aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca gaccatattg atgcaatgga
    12601 tcaattacgt cagtcaatcg gcttacgtgc ttatggacag aacaatccat tagtcgaata
    12661 tcaaacagaa ggctataaga tgtttgaaga tatggtaggc gccatcgagt atgaagtcac
    12721 tcgaatcttt atgaaagctg agatccgtca aaacgtacag cgtgaacagg tttctcaagg
    12781 aacagcttct cactcagaag acggagatgc caaagaaaat agtaatacta gcgcgaaaaa
    12841 acagccaatc catgtgaaca aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcatg gaaaaaatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt tttttaagaa ttcgtctcac cttctttctg ttatttgctg attctgttag
    13021 actagatata atgaaaaaag tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt
    13081 cgaaatctcc tgacagaaat gcaggaaaag gtcacaagct tcaggaggtc tctttgactt
    13141 agaccagctg gaagaagaca ttgcggaagc agaagcaaaa atggctgaac ctggtttttg
    13201 ggatgatagc gaagctgctc aaaaagtgat cagtgaaaac aatgcaaata aagaaactta
    13261 tgatcaattc cattctcttg ctgaagaact ggaagaaatc gaattgatgg tggagatgtt
    13321 gcaggaagaa cctgatgcag ccatgcaaga agaagcagaa acacgtatca atgcgctaga
    13381 tgaaaaaatg aagacatatg aattatctat gttgttagat ggtccttatg atcgaaacaa
    13441 cgccatcgtt gaattgcatc caggggctgg cggaacggag tcccaagatt ggggcagtat
    13501 gcttttacgt atgtatacac gctgggcaga gcagcatggg tatcaagtag agacactaga
    13561 ttatcaagca ggtgatgaag caggaatcaa gagtgtgacc ctactgatca aaggacacaa
    13621 cgcatttggt tatctgaaat cagaaaaagg tgttcatcgc cttgtacgta tctcgccttt
    13681 tgactcagcg aaacgacgtc atacttcttt ttgttcggta gatgtgatgc cagagcttga
    13741 tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa atcgatacgt accgagcaag
    13801 tggtgcaggt ggtcagcaca tcaataaaac agaatctgct gtacggatca ctcacttgcc
    13861 tacaggaaca gtagtagcta gtcaggcaca acgttcacaa ttgaaaaacc gtgagcaagc
    13921 gatggggatg ttaaaagcta agctttatca attagaagtg gaaaagaaag aacaagaagc
    13981 agctgcactt cgaggagaac aaaaagaaat cggctggggt tcacagatcc gttcttatgt
    14041 tttccatcca tattctatgg taaaagacca tcggacaaac gtagagacag gaaatgtcca
    14101 agcagttatg gacggtgacc ttgatttatt tattgacggc tatttaaaaa tgcatctaca
    14161 ataagaaccc attgttaaat tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat
    14221 tgccatgcta taatagctaa agactgaaaa agatatggag agaacgccat gattgaaatg
    14281 aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta tccgcaatct ctcaattgtc
    14341 atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg gagccggtaa atctacattc
    14401 atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat
    14461 gatctaatag caatcaaaaa ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg
    14521 ttccaagact ataaattatt accaagaaaa acagtatatg aaaatgttgc gtacgctatg
    14581 caagttatcg gcagaaaacc tcgggatatc aaaaaacgag taatggaagt gctggacctt
    14641 gttggtttga aacacaaagt tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa
    14701 cgtgtctcta ttgctcgggc aatagtgaat acgcctaaag tacttatcgc ggatgaaccg
    14761 acaggaaatc tggatccaga aaactcatgg gaaatcatga aattattaga tcggatcaat
    14821 gcccaaggaa ctactgtcgt gatggctaca cataacagta cgatcgtaaa tacgattcgg
    14881 caccgagtaa ttgctatcga aaacggccgc attattcgag atcaagcgga aggggaatat
    14941 ggatacgatg attagaacct ttttccgcca tttattagaa agtatcaaga gtttgaaacg
    15001 taacggctgg atgacagttg cttcagtcag tgcggtaacg atcacattga ctttagtcgg
    15061 tgtattcatg gcagttatca tgaacgcaac taaattagct caagatatag aaggaaacgt
    15121 tgatgtctct gtattcgtag atatcggtac aaaagacgaa gagatgaaaa agctagaaaa
    15181 agaattgaat gacttagatc atgttaaaaa agttgaattt tcaagcaaag aacaggaatt
    15241 gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg tttgaaggtg acaataaccc
    15301 gttatatgat gtgtatgtag taagtgctac tgatccttct tatacaaaac aagtagcaga
    15361 agaagcagca gatttgaaga atgtttctcg ttctgattac ggtggagctt cctcagatcg
    15421 gattttccaa atcgctggtg cagttcggac atggggatta gttgcagcgg cattgctgct
    15481 gttcgtagcg atgtttctca tttcgaacac gattcggatc acgattttgt ctagacagcg
    15541 tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga tatattcgct ggccgttttt
    15601 ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt ccgattttga tcatcacatt
    15661 tggttatgca tggtttttcc aattgatcaa tccatcatta ttacgttccc attattcatt
    15721 gattcatcca caaaatatcg tttggaaaat caatctttta atggtcggca ttggtgtgat
    15781 cattggttca cttggctcga tcatttctat gagacgtttc ttgaaattct aataccacaa
    15841 tataaataaa atgagaaagt tggacagttt cgttccagct ttcttttttt attggctagg
    15901 agaatagaag gaagcggctt ttttcccttt actttaaaaa acaacttggg tattcttaag
    15961 ttggtagaag aagaggtgaa gccgtgaaaa ttatcgttga caattttttt acgatccttt
    16021 tgattatgaa tattttgctt tcctttatca tcgtttttcg agaaagaaag gaaactgctc
    16081 aaacatgggc atgggtgctc gttttgatgt tcattccagt tgtaggattt attttatata
    16141 tttttttagg aagagggatt tccaaagaga aaatctttga tttgaagatc caagggaaaa
    16201 tcggtaagaa cctggaaatt gaagaagaca aacaggcatt gatgcgaggg ctttatcctc
    16261 atccgcctac tggacaagta gatgtgaaac aattgatcta tatgctcacg gtatttgaaa
    16321 gtacgctcta tacgactaaa aatgaaatca ctttatttac agatggtcgt gaaaaattcg
    16381 atgcgctgat tgaagatatc aagcaggcag aagaccatat ccacttccag tattatattt
    16441 atcgcagtga tgcattaggt gaagaagttc gagatgcatt gacagaggcg gcaagacgag
    16501 ggggaaaagt cagagtcctt cttgatgcat ggggttccac acaagtttct tccagctttt
    16561 ttcagaattt gaaaaaagca ggcggagaag tcgcattttt ctttcctcta tttgttccgt
    16621 atattaatcc tcggatcaat tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa
    16681 tcggatatac aggtgggttc aatgttggaa atgaatatct aggattggta aagaaatttg
    16741 gttattggcg agacaatcat ttgcgtattt acggagaagc ggtgtatacc ttgcaaaacc
    16801 gttttttgat ggactggaat tctcagcata caaaagaagt aggctattct cccaaatttt
    16861 ttccgtccat tcactctact ggtgatatag ctgttcaaat cgtgacaagc ggaccagata
    16921 ccgaacatga acaaatcaaa atgacttatt tgaaaatgat ttcgttagcc aaacgggaga
    16981 tattgatcca aacgccttat tacataccgg atggatcgat tcatgaagca ctgaagttgg
    17041 ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa caaaccagat catcttctcg
    17101 tttactgggc aacgtattct ttcgctgcag agctaatcga gtatggagca aggatcgaaa
    17161 catatgagaa tggatttatt catgcaaaga cgatgattat cgatggtggc attgtttcag
    17221 ttggctcagc aaatatcgat gttcgttcat ttcgcttgga ctttgaggtc aatacactgg
    17281 tttatgatga acgaatggct tcacgtgtaa gaaaagcttt ttttgaagac tcgaaaattt
    17341 ctacgcatct cacaaaagaa atgtatgaaa accgaggaat aatcatcaag atgaaagaag
    17401 gattagcaag attgatttcg ccgctccttt aaaacataaa aaggacatga gaaccatcaa
    17461 cagatggaga tctcgtgtcc ttttctaacg gggccatttc tgagagtacg tcagaaatgg
    17521 agaaagcgtc tcgcatgagc gaaattaact ttggggtatc tcttgttttt tggggaaagc
    17581 aagaaataag ctaatatatg ctattaaaag agacacattt caaagccagt atagcataga
    17641 aacttttaat gagatagttt tttttacaat tatgagaatt tattttgtta tttaatgatt
    17701 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
    17761 gatttctaat gaaaaaactt aaattattct taccaataat aggattagct attcttttat
    17821 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
    17881 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
    17941 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
    18001 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
    18061 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
    18121 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
    18181 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga agcggaaccc
    18241 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
    18301 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
    18361 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
    18421 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
    18481 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
    18541 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
    18601 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
    18661 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
    18721 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
    18781 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
    18841 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
    18901 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
    18961 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
    19021 ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc atgacggaga
    19081 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
    19141 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
    19201 ttggcgggac tgggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
    19261 cgactgtcac aacgatgact gttgatgcat taaaggcagt gccacggcac tatcgggaag
    19321 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
    19381 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
    19441 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggtctt actactcctg
    19501 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
    19561 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
    19621 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
    19681 taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga ttttagcagc
    19741 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
    19801 accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta actcgattta
    19861 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
    19921 ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga ttgaaatctt
    19981 gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
    20041 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
    20101 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
    20161 attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc cagaagcgac
    20221 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
    20281 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
    20341 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
    20401 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
    20461 cgtgttgatt attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
    20521 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
    20581 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatcta
    20641 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
    20701 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
    20761 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
    20821 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
    20881 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
    20941 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
    21001 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
    21061 gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga tatcttgctg
    21121 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
    21181 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcagct
    21241 cgtatcagcg actatactgc ctttttctat ttaggaaaag taatggaata tgacaaaact
    21301 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
    21361 ggttaagacg gaggcctcaa catgacaaaa gagattattt catctaaaga tcttcatctg
    21421 tattatggaa agaaagaagc actaaaaggg atcgatctaa gcttcaatca aggggaaatc
    21481 actgcgatga tcggtccttc tggttgcgga aaatccactt atctgcgttc tttgaaccgg
    21541 atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa agacaaagac
    21601 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
    21661 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
    21721 ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
    21781 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggtgggcag
    21841 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
    21901 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
    21961 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
    22021 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga tacgaagaag
    22081 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
    22141 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
    22201 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
    22261 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
    22321 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
    22381 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
    22441 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
    22501 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
    22561 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
    22621 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
    22681 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
    22741 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
    22801 ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc gtttgtttag
    22861 gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag taataagaac
    22921 agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta ccgcagatgc
    22981 aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc acaaaccctt
    23041 ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat cactatattt
    23101 tttatttgtg tgcttacttc atgggttatt cagcatcaag ccgctttata tgaagtacct
    23161 gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc aattgattac aggaaaactg
    23221 atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca aaaaaatcaa
    23281 atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca tggagatgaa
    23341 tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag tatcttcatt
    23401 ggcgccatgt tgcttattgg tggaaaacag ggtctatttg ctttaggaag tctcatcatc
    23461 aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg gatttctcta
    23521 ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt ggctttagat
    23581 ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt ttttttagca
    23641 ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg ctatgaggaa
    23701 ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact gattggaaca
    23761 gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga attgactaag
    23821 cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt gggtcgagat
    23881 gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc agttccatcg
    23941 tttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca
    24001 ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat ccctatctcg
    24061 gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt atacttgttg
    24121 ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat attcttttag
    24181 gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat cagaagtggc
    24241 cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc ttatttttta
    24301 tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc tttttatttt
    24361 gtttcctact attgcttcct atcactgatt ttttagcaat ccaaggtttt acatcggtag
    24421 aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga ttacttacga
    24481 gatcgttgct tctgattagt cttagtggtg ctgttttaga tgcgagtgta gcaatttcta
    24541 gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa ttgcgccatt
    24601 ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt ctttttgcat
    24661 tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt ccttttcagc
    24721 aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt accacattgt
    24781 ccgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt acaaaaaaga
    24841 aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt tttactgaat
    24901 cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag gaggcaattt
    24961 ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt tcgactgaag
    25021 aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa atcaaaaaag
    25081 aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa ttgatcgata
    25141 aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa agagaaagac
    25201 aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg aataaaactt
    25261 cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca cgcaatgaga
    25321 aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg acagaagaag
    25381 aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct agtggcgata
    25441 cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt aagaatcaat
    25501 ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat tggaaagatc
    25561 aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga tcacagttag
    25621 gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac aacatggaat
    25681 ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag catgaatttt
    25741 actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga aacgtgacgt
    25801 taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa
    25861 tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt aatgaagatc
    25921 atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc tacttaccta
    25981 aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg attgaaacat
    26041 tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg gaccaattaa
    26101 ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga aacgggaaca
    26161 tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg acagcagaaa
    26221 acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa gataatttga
    26281 aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca gatggcatcg
    26341 gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg tccaattacg
    26401 aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt cgtgtttcag
    26461 atggtgaaat cgcgcaagtg caactttcaa cgacaacagg aagtatctat ttaaaagata
    26521 cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga agcgattttg
    26581 taaaatagct tcccctattt tacaaaattg tgccttattg ccgatagtct atctcttgaa
    26641 caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag gaataatttc
    26701 agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt ttgcagaaag
    26761 acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag tagaacagag
    26821 gtgagacatg atgaaaaaaa agttgacaaa atctaataaa aatgttgttt tgacagggac
    26881 tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg tgctttatgt
    26941 attcctgagc ttagttgctt ttggaagtcc aattatttta tacattttat tagctttgat
    27001 tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat attatgaaaa
    27061 aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat
    27121 cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt agttaatacg
    27181 ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag cagtatttgg
    27241 acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa accgattttg
    27301 acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt tgttatcaat
    27361 gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag tttttcttca
    27421 ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa
    27481 cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt ttttatttag
    27541 aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt tttaattggt
    27601 agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa atgaggtagc
    27661 atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt tggaaatcgt
    27721 gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct cacgcccagg
    27781 actagagttg actggttatt ttaattatta ttcccataat cgtttacagc tatttggcag
    27841 taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca tcatgagaag
    27901 attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccag ctccagaaga
    27961 aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca tctcaacttc
    28021 tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc gaacaagtgt
    28081 ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg acagtgggat
    28141 aggaaaaagt gaaacagcgt tggagttgat caaacgcggc catcgattga tcgcagacga
    28201 tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct
    28261 ggaacatctg attgaaatcc gaggaatcgg gatcattgat gtaatgaatc tttttggtgc
    28321 gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag catgggcaaa
    28381 agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg cggaagtggg
    28441 cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt
    28501 agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa catttgaaga
    28561 aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag tgacaatatg
    28621 ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta ctggtatgca
    28681 ttgatcatcg tatcaggtat cattatcgct atgtggctta gcagcagaga ggctgttcgg
    28741 gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc tatctccatt
    28801 attggcgctc gcttatatta tgttttattt gatcttcccc agtatatagc tgatccaatc
    28861 caaatattca atatccgttc aggaggctta gctatttatg gcggactgat tgcaggcgcg
    28921 atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt tttagatatc
    28981 gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt catgaaccac
    29041 gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt accgcaattc
    29101 atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct ttatgaatca
    29161 ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc acaccttttg
    29221 aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg tttctttatt
    29281 gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc tcaattatta
    29341 tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa aaaaggaaat
    29401 ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt tttagagcgt
    29461 taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg ttttaggacc
    29521 aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg acgttcggat
    29581 ttggggacat catgcttcac aagtagaaga aatcaacacc caacacacga atcaacgtta
    29641 tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg aagatgcagt
    29701 aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa
    29761 agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta aaggattaga
    29821 acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag cagaaaaaag
    29881 aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcaa aacatgacat
    29941 cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac aagagctgtt
    30001 catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag agatgggggc
    30061 agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag ggtttggcga
    30121 taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt taggtgtagc
    30181 aatgggtgcc aacccgctga cctttatcgg attaagcgga ataggcgatc tagtcgttac
    30241 ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac aagggcagcc
    30301 cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata ctacccaagc
    30361 agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc atcactcaaa caatctatga
    30421 agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg
    30481 aaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta tatgaaagtt
    30541 aaaaaagcag ttattccagc agcaggatta ggaacaagat ttttaccagc aacaaaagct
    30601 atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat cgttgaagaa
    30661 gcgttgaaat caggtatcga agacatcttg atcgttacag gaaaagctaa acgaccaatc
    30721 gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa aggaaagacc
    30781 gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat ccgtcaatct
    30841 catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg aaatgaacca
    30901 tttgtcgtta tgctaggcga tgatataatg gaagacgaag taccattgac acagcaattg
    30961 atcgatgact atgagcgtac acacgcttct acgattgccg ttatgcaagt tcctcacgat
    31021 gaaacttcaa aatacggaat catcaaccca ggggaagtac tggaagatgg gttatacaat
    31081 gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt agctattatt
    31141 gggcgttact tattgactcc agaaatcttt gatgtattat cagaacaaca accaggtgct
    31201 ggaaatgaga tccaactaac tgacgcaatc gatacgttga ataaaacaca acgcgtattt
    31261 gcccgtaaat tcacaggtaa acgttatgac gttggtgaca aatttggttt tatgaaaaca
    31321 agtattgaat atggtctgac acatccagaa gtaggggcac ctttgcgtga atacatcatg
    31381 tctttaggta aaacattaga agcagaagga agcaagatca atcataatcc aaaaggaata
    31441 acagaaatca aagaagaaaa gaaaaaataa tctttttttc taacgagctt cagcggctaa
    31501 ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa aatgctatcc
    31561 ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa ttgcaggatt
    31621 gatcgcagca atcgcctttg cgatacttgt cgttttcatc gttcgcgtat tgttgcaagt
    31681 ttccaaaact ttagaaaaag tagaccagac tgtcgcagaa gcaaatacga caattgagat
    31741 cgtcacacaa gacgtagatc ttttatctag acaagtagaa ggcttgctag tcaaaagtaa
    31801 tgagctgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt tcacagcagt
    31861 tgctgattta agtgaaagtg tatcggaact taatcattct agtaaaaaca tcgtgacgaa
    31921 agtcggcgga atcggaaaaa cgacagctaa agcgactgtt gccggaaaag tcggtggaac
    31981 agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa aataagaatg
    32041 gctaaaaaag gtggattttt attaggtgca gtagttggag gtacagccgc agcaatcgcc
    32101 gcattattgt tagcacccaa atctggtaaa gaacttcggg atgaattggc tgctcaagca
    32161 gatgatttga tggacatggc ttcagattac acagatatgg caaaagaaaa atcatctgag
    32221 ttaacaggta ttgcaaaaga aaaagcgcaa ggtctttctc aacaagcagg ggatctagca
    32281 ggcaatgtta agcagaaggt aagtgaatca gcagaggaag caggaagtga atcagatgat
    32341 atcttgttaa acctcaaaga acaatcagct gatttatcta atcgtttcaa aaaaacagca
    32401 gaagatctga aaaagccagc agacgaatta ggtagtattg ctaaagatac ttcagaagat
    32461 atcatgatcg atgtaaaaga tgctgcagat aaagcaggag agacggtcaa tgaaggaatg
    32521 tcagaagcaa aaccagttgc agatgacaca aaagaaatgt tgaaaatggc aaaagaagat
    32581 gcaaaagaag ctaaaaaaga aatgaaagat acaatgacag attcgactac caatcactaa
    32641 gattactcat ttctagaata aaaaaggtga gagcggacca gctctcacct tttttatttt
    32701 cattactcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca accatcactt
    32761 gtcacataca cacaatcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcaatc actaagtcgt ttccttctac aagagaaggg
    32881 aattcgtgga cggttgttcc gattccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctggg
    33001 cgaactgcct cttgtgcggc aagctgtgct tcaagagtga tgttatagat tttttcttgg
    33061 aactcagttg gtttttgata agcaactgta cgagtagcat cactgcaata gccattccaa
    33121 ataacgccaa gatcgaataa cactagttca tttggtgaaa ccttcgtgct tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt tttttaattg gtattcgatt tctgcgataa ttgcttgttc agttacgcct
    33301 tcttttactg ttttgaagcc aatttcaaag gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcttctac cgttttgatc agttgcagac gctggataat agaagttaga
    33421 tcggtgtaag aatctgtacc tgtcaagata tcagagatag catcgaagcg atcgacagtc
    33481 agagcattct tttcaatacc tacattacca ggttgcccat agcgtgcgcg aagctctttt
    33541 ccgatgatct cccaaggatt ttcgctgtct aagtagccac gtacatcgta cgcccagctg
    33601 ctgttttgag catcttcaac ttccagagca ggagtgaaaa ggaaaggatc ttgttccaga
    33661 gggataaaaa gagctagtac acgctcgtga ggatcacttt catatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tctacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattg attaaaagac caacgttctt aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattgttgag attatttgat atcctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt caaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa tgcagtcgca cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggggtaa tcattcctga tgtcagcaat atgttctttg cctctttagc
    34261 acgtggtatc gacgatgtgg caacaatgta caaatacaat atcatcttag caaactcaga
    34321 tggcaacgat caaaaagaag tcaatgtatt gaataattta ttggctaagc aagtggacgg
    34381 cgtgatcttt atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aacgcctgtt gtactagctg gttccattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgtg tcagcaacaa aagatgcagt aaataaatta gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagcggtg cgctgattga tcctatcaat ggtcaaaacc gtctaaaagg
    34621 ctataaagaa gcattaagcg aaaacaatct accatatagc gaaggattga tttttgaggc
    34681 acaatacaac tttaaagatg gtctgtctct agttgaccgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagacg atgaattagc cattggtatc ttggatggtt tattagaccg
    34801 cggcgtaaaa gtaccagaag aattcgaaat catcacaagc aacaattctt tactgacaga
    34861 agtagctcgt cctcgtttga caagtatcac acaaccatta tacgatttag gagcggtatc
    34921 catgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatcaac taaataataa aagatcaatc atttaaaggt
    35041 tgttccaaaa gtggtcaact tctggaacaa cgtttatacg agaaaggggg ttatgacaaa
    35101 attttgtcat aacccctttt tttttgtgac tttaaaaaaa gagaaaatgg cggaaacaaa
    35161 ccttacgtcc atcgtatacg acacaatctg tgcgtagatg ctacattttg ttcactacta
    35221 tcaaaattac tttaaaatga aaacgtgata aaaaagaaag tagaaaagta gataagtaac
    35281 tctaaaaaat ccaaaaaaat ccaggctcga ttgcgtactt atcttaaagg agaaacaatc
    35341 catcatatat aacccgaaag gagacttcta tttgcctaag aaaagacgaa agaccatagt
    35401 catcattttg accattctta ctataggaat cggagggaaa ttttacatgg acaaaagaga
    35461 aatgcaaaga cagaaagatt tactcgcagt agaaaaacag tccgtcaaag ttctgaaaaa
    35521 tacctttgcg gatatcaagg aagtgaagat tgaaaaatct gctcgaaatg aaatgacgtg
    35581 ttcataccgg atagttattc taatgactaa taaacaagat caatcgattt atttttctta
    35641 tagtttttgg aaagaaagaa atgagatagg aagttacgga atagttgatg aaaaaaagca
    35701 aaaagaagga aatacgctca ataaagttaa agtaacttac tctaatggaa atgaggaaag
    35761 tatttaatga ttactgacaa acaatatagt gaattaagtg atgctgttta ctggctagat
    35821 cctaaacata gagactatgt acctgaaatg caagaaaatt taagttttaa aattaacggc
    35881 actatgtata aaatcctaaa aatcaaaaac tccttcgacg gcatgcaggc gatggctgta
    35941 gcgccaatcg tccatagcaa attggagaaa aatttcaaaa ataaaaaaat tcctgcaaac
    36001 tttcgcgtac ttaaataatg aaggtgtcaa tacttacata aaaagaaagg aggcacgttc
    36061 ttgtcaagga aaataagaaa actcagctta gccattttat taatcgtcat attagggatc
    36121 ggagggaaag tctatatgga caaaagagaa gcacaaaggc agaaagattt actcgcagta
    36181 gaaaaacagt cagtcaaagt tctgaaaaat acctttgcgg atatcaggga agtgaaggta
    36241 gaagaattta aaaaaaaccc tgttaccgga agttatggtg cacttgtgac aatgacaaat
    36301 aatgaaggga aatctgttta ttttgattat agtttttgga aggagagaaa tgaacttggt
    36361 ggttttggca ttgagaacag agcggtacaa aagacaggag tcactataaa aagagtaaaa
    36421 gtaatttttt ctaacggaca ggaggagtta gtatgacaat aaacgataag cattacaatg
    36481 atattagtga gaaggtttat tggctagatc ccaaatatcc ccgctacaat gagggataca
    36541 aaaagaactc tgttaaagaa tttgctggta tggaatttca aatcctacaa atcaaagact
    36601 cattggacgg catgcaggcg atgtctgtag cgccaatcaa gaacggtaaa gtggatcttt
    36661 ctcaagtagt gattgctttt gcaggaacga acttaagtga ttggaaagat ttggaaacgg
    36721 atgcaagaag tatcatgaat ctctacggga atagaccagg agcaagtttt tctgaaaaag
    36781 caacaacaaa caagatttta tccgttagtg gattgaaaac agcggggcaa atcaattcag
    36841 cgattgcatt tgctaatgaa gtaaaaaagg aatatggaga agctgctatt tctactaccg
    36901 gccattcatt aggtggattc ttagcattat atgtagcagc agagaaacaa tggaaaaatg
    36961 tcggcttcaa cgggccggac ccgtatgatc tcttatcacc agaagcaaaa aaatgggtgg
    37021 aagaaaatcc cggtatgctc accaattacc gaaatcacgc cgattttatc ggtaattttg
    37081 gtggtaatgg cacaggtgct gaagttcgga tcagtatgga aacagcattc ttgaatctgc
    37141 ttgcttccca cagtttaacc tcttggaagt ttgatgaaaa tggaaagctg attattcctg
    37201 aaaatgaaaa caatgttaaa gctcgaaaac aacagaaaga aaaccaagcg acccagttgt
    37261 tttcctatca gttgcaagaa ctgaacaaca tacgtaaaaa actacgatta agtggcggcc
    37321 agctgacagt taatgaacaa atttacttag atgacacaca agctaggtta gtagtggaaa
    37381 agttatcggc aaaaatgaaa agcgctatgg aagaagttat ccgcatcaat caaagaggaa
    37441 tttttgagct agagaaaaaa tggcaagaag gcttgcggca agcacgtgtt gtagcccctt
    37501 cgttgactga acaggaaatc atagaagcat tagctagtgt gggcgccaca aaagaaaact
    37561 tagtcgattc tgcgaaagaa gtctatctgc tacgaatcaa taaggcaaga cgtatgggtg
    37621 aggcctttga tacattggcg aaggagatcc aagacaaaat caatgaaatc gtggcacgag
    37681 atcgagaatt agcacagcaa ttcaattaaa ggagaagtag atgaaactag aagaattgga
    37741 aagagattat cgaagtgaac gaagacaatt agaagctcaa gaagatgaat tgatccactt
    37801 taagaatcaa ggattacgat tgatggagca agaggagcag cgactgcatc atgtattaaa
    37861 gaacttagct ttagataatg agcctctgtt tcaagcaaga cgaagttatt cgaattatca
    37921 ggagcattat caacaagccc tacaagtaga acgacgaaaa ctctatcagc agcaagatga
    37981 actagaacgt tcctatcgtc aaaaaatcag acaaataact atggagaaat catcttatga
    38041 atgaattttt aatgcaatta ctaaaagaat tagagaaaaa tctacatcga tcaaatgtac
    38101 gttcagttgg tatggggata tctcagcaaa tttgggcaga aaaacgattg acagatcaaa
    38161 actaccaaat gactgcagaa gaagctgctc aagaattttc taaccgacat gtacacttga
    38221 aaggatcagt aaaagggcaa tttgcagaaa aactaactga attattccaa agacaagaaa
    38281 agaaattaaa acagtttcgt tagtaaagtt atatagaaaa attttgtgaa aat
//
LOCUS       DAFFQY010000019        36939 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFFQY010000019 DAFFQY010000000
VERSION     DAFFQY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.019"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(94..522)
                     /locus_tag="LRB02_000883"
     CDS             complement(94..522)
                     /locus_tag="LRB02_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659106.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(550..1188)
                     /locus_tag="LRB02_000884"
     CDS             complement(550..1188)
                     /locus_tag="LRB02_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659107.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1192..3174)
                     /locus_tag="LRB02_000885"
     CDS             complement(1192..3174)
                     /locus_tag="LRB02_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL4659108.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3354..4019
                     /locus_tag="LRB02_000886"
     CDS             3354..4019
                     /locus_tag="LRB02_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659109.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5954..9103)
                     /locus_tag="LRB02_000887"
     CDS             complement(5954..9103)
                     /locus_tag="LRB02_000887"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HBL4659110.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9116..10315)
                     /locus_tag="LRB02_000888"
     CDS             complement(9116..10315)
                     /locus_tag="LRB02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL4659111.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10305..11900)
                     /locus_tag="LRB02_000889"
     CDS             complement(10305..11900)
                     /locus_tag="LRB02_000889"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL4659112.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12484..13191)
                     /locus_tag="LRB02_000890"
     CDS             complement(12484..13191)
                     /locus_tag="LRB02_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659113.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13256..14092)
                     /locus_tag="LRB02_000891"
     CDS             complement(13256..14092)
                     /locus_tag="LRB02_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4659114.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14110..15519)
                     /locus_tag="LRB02_000892"
     CDS             complement(14110..15519)
                     /locus_tag="LRB02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4659115.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15533..17356)
                     /locus_tag="LRB02_000893"
     CDS             complement(15533..17356)
                     /locus_tag="LRB02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4659116.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18199..18525)
                     /locus_tag="LRB02_000894"
     CDS             complement(18199..18525)
                     /locus_tag="LRB02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659117.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18571..18933)
                     /locus_tag="LRB02_000895"
     CDS             complement(18571..18933)
                     /locus_tag="LRB02_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL4659118.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18970..19185)
                     /locus_tag="LRB02_000896"
     CDS             complement(18970..19185)
                     /locus_tag="LRB02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659119.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19312..19647
                     /locus_tag="LRB02_000897"
     CDS             19312..19647
                     /locus_tag="LRB02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659120.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19723..21720)
                     /locus_tag="LRB02_000898"
     CDS             complement(19723..21720)
                     /locus_tag="LRB02_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659121.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21818..22093)
                     /locus_tag="LRB02_000899"
     CDS             complement(21818..22093)
                     /locus_tag="LRB02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659122.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22326..23060
                     /locus_tag="LRB02_000900"
     CDS             22326..23060
                     /locus_tag="LRB02_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659123.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23099..24241
                     /locus_tag="LRB02_000901"
     CDS             23099..24241
                     /locus_tag="LRB02_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4659124.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="LRB02_000902"
     CDS             complement(24336..25727)
                     /gene="sufB"
                     /locus_tag="LRB02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL4659125.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25732..26202)
                     /locus_tag="LRB02_000903"
     CDS             complement(25732..26202)
                     /locus_tag="LRB02_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL4659126.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26189..27424)
                     /locus_tag="LRB02_000904"
     CDS             complement(26189..27424)
                     /locus_tag="LRB02_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4659127.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="LRB02_000905"
     CDS             complement(27421..28707)
                     /gene="sufD"
                     /locus_tag="LRB02_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL4659128.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="LRB02_000906"
     CDS             complement(28720..29493)
                     /gene="sufC"
                     /locus_tag="LRB02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL4659129.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29690..30538)
                     /locus_tag="LRB02_000907"
     CDS             complement(29690..30538)
                     /locus_tag="LRB02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL4659130.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30562..31248)
                     /locus_tag="LRB02_000908"
     CDS             complement(30562..31248)
                     /locus_tag="LRB02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659131.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31241..32275)
                     /locus_tag="LRB02_000909"
     CDS             complement(31241..32275)
                     /locus_tag="LRB02_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659132.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(32338..32594)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32670..33008)
                     /locus_tag="LRB02_000910"
     CDS             complement(32670..33008)
                     /locus_tag="LRB02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL4659133.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33010..33366)
                     /locus_tag="LRB02_000911"
     CDS             complement(33010..33366)
                     /locus_tag="LRB02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL4659134.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33372..34559)
                     /locus_tag="LRB02_000912"
     CDS             complement(33372..34559)
                     /locus_tag="LRB02_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4659135.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34750..35595)
                     /locus_tag="LRB02_000913"
     CDS             complement(34750..35595)
                     /locus_tag="LRB02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4659136.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35829..36794
                     /locus_tag="LRB02_000914"
     CDS             35829..36794
                     /locus_tag="LRB02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL4659137.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10954 a         7583 c         6122 g        12280 t
ORIGIN      
        1 acaactaaaa atgaatatat taaaactacc aaaaataact tttaacgctg tttcattata
       61 tgttttttct attttatata aacttttgga tacttaatac caaccggttg gattgtacca
      121 acatgagaat gtgtcagttc tatttcctgt cgcacttgca taggcccata ctccttttgt
      181 tgcatttgct gatcgaatag agtttccaac tttcgctttt gcaccgtgac gattatagac
      241 acgactatag tggttagaat gacctgtttt tctcgtacaa gttgtatcac tataataaac
      301 ccatcttcca gagctatgat taattgctgt tctagaaatt tcccggtcta ctggtaaatt
      361 tgacttaata atattacctt tagcatcaaa atgaatctct cctggcttta aagatttgta
      421 atagatttca ccgtgaactg aatttgcttc cacttcagat gacatagcgt ttcctccaac
      481 aaaacctatc cctagtgcac aaataataac tgcttttttc ataaaacgac atcctttcct
      541 ttttgatttt taaagcatga tcttcttgtt tcctattttg gtcagatatg gatcatgcgt
      601 gactacgata actaacgtac cttgggaagc ttgttgcgat aagatttcaa ctactttatc
      661 cttatttctt ttatccaatg acccagtcgg ttcatcggct aaaatgattt tgctattctt
      721 caataagact ctcgcacagg cgattctttg cttctcacca ccagataatg tgtagatagg
      781 cgtacttagc ttcttgttta tatttacttt tttcaatgcc tcatactttg ctgttcgctt
      841 atctgaaaca tttaaattcg aaaacttcaa tccaatatct aaattaaaat cgattgtttc
      901 atattcaatc aacccaaaat cttgaaaaag aaaagatatg gtatttcttt ttatcaagcg
      961 cttctctctc ccagttaatt gtttcgtctc ttgatcgaac aaatagaatt gtcctgaact
     1021 gaactgatca ttcaatccta atatattcaa taacgtactt ttacctgctc cactttctcc
     1081 aacaacagtc acaagatccc cagcatccgc agtgaaacga tccaaatgaa gaatcgtttt
     1141 gtttcgaatg atttttttcg caccttttat cactatcaac ggctccaatt gttaacctcc
     1201 attcatcagc aataactgac ttttcttttc tgtagacaaa ataaaaacaa ctgaaagaat
     1261 aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat ttaggctctc tttctataag
     1321 aaaaaggaag attacacttg tcgttcccca tttgatgagg ttcatcatga agaaatcttt
     1381 atacgtttca aagagcgaat aaccacttga acgaaagata gcgattttcc gacaattgta
     1441 gacaaaatat atgcataaca tgacatagct caacaagaag aaagaaaaga aagtgagtaa
     1501 aaatatctgt atcaagtaat gaaatgatct gcttaacact ttgacagcta tctcttcagt
     1561 ctgagtatac ggaaatgata actgcaagtt atcacgtaac tgactttctt caatcagctc
     1621 aaataacctt tctttcgaca aagagcctat cttgatcttt aacggtggat agatttctcc
     1681 gctgagaata tttctttcca aatacatact gtttttcaat gtcagtattt caacaaccgg
     1741 ataatgttcg atccagggga tagaaggaat aaatgtataa atgggttgat tgttttttat
     1801 aaatagaaat ttcggttctt tttcactgat ttcttgataa tattctgtta tttgcgccag
     1861 gtcatttttt aaatctgtat gtctatatcg ttcagggatc aatataacag ggtgcgtttc
     1921 tttttcggtt atttggatca accttccaga gacatcaatc aaaggaaatt tcttaagata
     1981 attcagattt atttttatcc ctctgccaaa ctgttcattt tcttcattca agtaatctga
     2041 tacattgact aatatacttc cgtttttatt caaagtttca tataattcct gatctgctgc
     2101 tgatgcaaaa cgattggaat aaataaaatc cacgtggttt ttcccgatgg ttactggata
     2161 aaatacatga tagtcctcac cagcgatttc ttttggtggt tcatgggaaa cagctaactg
     2221 tgaagaagaa aaaacgatag ttgctaattc gacattggca acggtcaaaa gaagagaaaa
     2281 caacaatcca aatgtaaaaa gaatgtattg gatatagttg aacgcttctc ttaacgaagc
     2341 gattttttca actaataaaa gagaaatcaa agagatacca tatattccgc tgaacaagaa
     2401 aacgtacatc tggaacaaat aaagaaattg ctccttataa tacataaaaa taaaaccacc
     2461 aatgattcct gcaattatga atgtaataat gattttcttt ttccccaata aacaccacaa
     2521 tacttgatgt aatgaaaagt cattattcaa caataaactg atttctttat tcatcaataa
     2581 gaaaaaggat gataacaaaa ggaataaaag aatcaatcca atagttaaat acactcgata
     2641 attatatacg tctgtatttg aaataaccga atacgaatag ctattttcaa agtcttcaaa
     2701 aatattagtg gcataatcag tgtgaaatgc ttgattgaat cgatcagtga atagtgaaat
     2761 gacccgttca taggcttctt tttctctcgt catcaagaaa aattcacctt caaaatcttt
     2821 tattttgatc gattcttcaa tggacgttac gcgaaaggag gtattagaat agggtacctt
     2881 ctcgtgagaa gatttgacat ccccttgata atacaatgtc atttcaagct ttggctgtat
     2941 cttgaagttc caagatgtat ctacttcatt ggattcattt actgtttttt tcatataaga
     3001 aagctcagtt tctgatgaga cattttcaaa taccatgatt agctgctcat attcttcttg
     3061 cccaataaaa tgttgtggca atcgaatcgg agtataagta tctgtttgat ggaaaaattc
     3121 atttgtccga tagagtacct gtttatttat actaaccaag gcaaaaccac tcatgatacc
     3181 ggtcactatg attgtgagta aaactgtgaa cttttttttg attatcacca ctctccctta
     3241 cttaaatgat tctttgataa aatcataccg tttttcatca aaaaaacgta tctatcaagt
     3301 gtataggtca tgtagaataa atctatatac tgtagaatcg aaaggagaag aagatgaaca
     3361 ccggagaaaa aatacgaact taccgcagaa attgtaagct tacgcaaaaa gaattaggcg
     3421 aaaaaattgg tgtttccgat aaaacgattt ctagttggga aaattctcgt acaatgccag
     3481 actttgaaat gctgtcactt ttacatcata ctttaggtct gccgattgat tttccttcta
     3541 ttgaatcagc taccgaatca atagaaccaa cattgccaga tgaacctctt gttaacaaga
     3601 aatcttattc atcaaaacaa cccttctcac tcaaaaagac actatctttc tttctgctcg
     3661 tcttttgttt ggtctatctt tttcttatca attatcaata caaaaatgaa acgatcgatc
     3721 gattcaatcc ttttttgaaa ccacaaatcg gctataccac actccctgta ggatacgtgg
     3781 acaattccat cgaacgttgg gtatgtaatg atatctttgg ccatggtaca tggctgtctt
     3841 ttcacgctgg agaggtagaa aaagagaaaa accatgctgt aatcaaacac aaaggaaaac
     3901 acgtcatgaa cgttcgcctg ctcacttggg aagccattcc ctcagattac caagaaatcc
     3961 tcagccaaga ctatgacgac gccttaaaaa gaagccctca ggagccagat aggcattaag
     4021 taggagatga acgtagagat agaaaaaatc aatgcacatg atcttatcca aaaaacaggg
     4081 gtacaaatta attgaaaaag ccttgagatg aaagttccta aataaaaaag ttcagaatga
     4141 taggtatttc tggcttatag tataatattt acgccacagc ctcccaatga ttctttttgg
     4201 aatcataatc aaggatggac attcacaaaa atattttgct cattcgatcc gaatagatta
     4261 acgaccaatc tagtaagtaa tatagattgg tacgtagtca agctgcacat aatttagatc
     4321 aattttagtt cttttttata tttctatgat ctgctcattt acttttatat gatggcttac
     4381 agtaacataa ataaatatga taatcgctgg tagtttatcc aagagtactc aataaaatac
     4441 aaaatggatt gttcaagcgc ctatcataat gataaaatga aacaaataaa cgatacaagg
     4501 aggaagagac tttgagtaat ggcagaacaa ttttttcatt gtaaaaattg ttcagttgtt
     4561 aatctatggg atttcggtag catgggcatt tttttataga ggaatcacac caacctggcc
     4621 gttttatctc ttgttagtgg gaatattagt aggaaatttt gcccctcttc actatcgtac
     4681 gaactttttt ccaactgatt ctggcctttt tcttaaaaca aagacgcgaa aattagaaaa
     4741 cattattgaa atgacaatta cctgtataat cattttctta gttatattcc taaacgtctt
     4801 ctaataagaa tctcttaaaa ttagcttgtt acagttagtt acctagttta tataacacag
     4861 ttatcttatc ttccttatca acatcttttg ccaactcttt gtcttaaatt cgaaacatat
     4921 aagcagagct acgaacacca agactgtttc ggctctgttt tttgcatcaa attcactctt
     4981 atatagctga taatctcggc tctttaaaag aaattatttt cgcttcattt tattataaaa
     5041 aggctaggaa ccttgatgtt atcaggttcc tagcctttat ttaagtatta ccccacagac
     5101 ccttccatat agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact
     5161 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
     5221 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
     5281 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
     5341 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat
     5401 cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga
     5461 gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg
     5521 agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca
     5581 aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa
     5641 accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata catttcactt
     5701 aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg
     5761 gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga
     5821 ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga
     5881 catctatccc tattttctac acttccactt gactaccatc tttgaatcgt acaattttct
     5941 taactcttct cgtttacctc aattcattct caagtggaat caactcatca tctaacattt
     6001 tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg
     6061 ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt
     6121 cacctggtag atattgcaga ttagaagcat gtaattcttt ctcagaaaca aaccactcat
     6181 tggaaaagac atgaatcaaa tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa
     6241 agatatcttc attactatct aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa
     6301 cttctttgac tccttctgtt ttattcaatt tagccaaagc tttttcaatt tcttctcgtg
     6361 catctgaact attcgcagca taagttccta gtaattgatc aatataagtt gaatcaatat
     6421 catacaactt agtggtattt tcactataaa attcgatcgt tgaaaaatct tctccttctt
     6481 tttctggttc ttcctctatt aatgaaccct tcacggtctc gtacacgcca atttgttctt
     6541 ccaaaagttg aatttgattg ttcaaagttg gagatttcac catgtcatca ttgtaatcgt
     6601 cataagtcac taacgattca taagcacgat tcaattcttg aaatgctttg acgtactcga
     6661 tacggatttc aagtggcgta tgttcatcaa tggattccat ggatgaaacc tcttttaact
     6721 taccatatgc ttgtttgaat ctttttttgg attcctgata acttggataa atcaaacctg
     6781 attcttcttt tgcttttgaa tacagttttg tcgcatcctc gacattcttc tccattgtcg
     6841 ctggcttacg aaacgtcaca atcaatcctt ttgtcttatc aggatacgta cgattcgtac
     6901 gtgaaaaagc ttggatcaat cctgcatact ctagattacg atccacaaat agggtttgaa
     6961 tcgtaggggc gtcaaaacct gttaatagac gatccactac aatcgctaaa tcgatttgtt
     7021 tcccaaattg tttgaattcc gctctttttc gtgccaaacg attattgatg tctccgttat
     7081 aacgctcaat gtctgctaat gaccaggaag tatcataata ctgattatat tcctcaataa
     7141 ttttttgcat ttcttcttgt tgggcacttg aatctcggct attttcttct aatgaatagg
     7201 taatagcgat tcgtggaaaa tcaggatcct ccatcgttcg tccttcacga atgagctgat
     7261 tgggaaactc ttttgttaac caatcaggct ctttcgtcat ctctttgatg gcccgataat
     7321 actttttggc catgtcaatg gaactcgtgg ttaagattgc agatttcgtt ggcctgccat
     7381 ttcgaaaatc aaattttgta taggcattat ctggacggaa cattttatga atgacttttt
     7441 ggatatgttc atctgcttca aaaacagctg ggtccaaata actttctttt ttgacaggtt
     7501 ccatttgatc aatcatacga ttgatctgtt ctgatgaata ctctgcatat gtttctactt
     7561 cttttaattt ccgatagatt ttattctcta gtgaagtcgg ctccacggtg ttttcatgct
     7621 ctacttgaaa tcctaagaca gagccatctt ctagcgcatt cttaatggta taggtatgca
     7681 agacctctcc atactgatca tgggttgtac gtgccaattg ccctttggct tgtttccgat
     7741 tctcaggaaa aatcggtgta cctgtaaagc caaaccaagt ggatttcgga aacatttttt
     7801 tgatttcttc catattttca gcacttaacg ctcgatgaca ctcatctact acaaacacga
     7861 tatgttggcc cattaatttt tggaagcgat tggttccttt tttctcttct tgtttctttg
     7921 catatttcaa cgccgcatct agtttttgac gggttgtaat gattacaaca tttgcattgg
     7981 catctgccaa cagggtttca cttaattctt tggcacttcc cgttccaacg atcaagctat
     8041 tcgctttggc gttcccggaa gaaatcccag tattaaattc cgaagcaaat tttgtaaact
     8101 ctgtggtggt ttgattatct aaatctttgc ggtccactaa catgatcgtt cgatcgatcc
     8161 ccgattttcg tgctaaaagc ttagtagata caaaactagt caacgtcttt cctgaaccag
     8221 tcgcatgcca cacataccct gattgatgtt tatttgcggc aataaacaag gcttgaatcg
     8281 catgtacttg ataaggatgt aacaccatga gggttttatt atcttgatct tcactgacga
     8341 ttgtataatc cgcaatcaaa cgatgagcat ccgggatatt taacacttgt ttacaaaact
     8401 catataagtt ttctactttt tcattgtctt tcgttcgcca actaaataaa aacttagggt
     8461 gcaagtcttt cggtaaggca tttgcaaaat accgagtggt ttgttcattg gaaaccacaa
     8521 acaactgtaa ggtcgaaaag atattgttcc gaaacatacc ttcttctgca tattttttga
     8581 tttggttaaa tgcctgataa acaccatctt ttgcagttac ttgtttcagc tcgatttgga
     8641 caatcggtaa tccattgatt aataaggtga cgtcaaaacg tcgatcccgt gcttctatgt
     8701 tacttccacg ttttgcaatt tgatggacca cttcataagt agagattcct ccaccaatgt
     8761 cttgattgga ataaaggata agtgagacag aacccaattg cgaatcttcc cgttcaatcg
     8821 tgatccgagc catcccattt tcacctttta accatttcgc agcatcaaat ggggttttcg
     8881 ttcgcagcag taattccgtt ttgatcgttt caaattcttt atcggtcaaa ggaaaatcat
     8941 tcagttctgc ttgattattt gagataattt tttggcgtaa attgacccaa aggtcttctt
     9001 ctgattttag atccggacga tacgtccact gattatgccc ttctccaagt acacgaatca
     9061 actgttcttc gacttctgct tcctcacggt gactaatttt cattcttctt ccctcctaga
     9121 caaacatctt ttgtaagaag ctttttttcg tttcttttag taaatctaac ttacgttgat
     9181 gaagagcgat cgtatcatct agctgtttga agaaagtacc gattttttgt tgttcatcct
     9241 ttacaggaac cttaacactg atattctcaa tatcagaact gttataagaa ggcaatgccc
     9301 ctacttggga gtatcttggc aaatttatag tttcaaacaa tattttacca aaatagatat
     9361 cccaagtatt attaaataaa taagccatgg tattgttatc tattaagaaa gggcttgtga
     9421 ccaaacgttt tctattaagc attattgctg cgccaacttt agcaaatata atcgctggca
     9481 catcttttat tggcttccat tttttacgct caatttgctc attagataca taatgatttg
     9541 catttctcat ttcatactca ttaccaatgt tattcatgtc ggaaacttta aaaaatggag
     9601 taccttcaga accaccttgc tcggcatcag gaaatccaat tcccgattga gttttaccta
     9661 attctcccaa cttacgctct tcccaatctt ccgtaaaacc aggaaaccgg atttctggca
     9721 cttttgctcc gttttcaggg aacatttttt gtaagaatcc ttttttcgtt tccttcagca
     9781 aatctaactt acgttgatga agagcgatag tgtcgtcgag ttttttgaag aatatgccga
     9841 ttttttgttg ttcttttaaa tcattgggaa tatagaatac tgtatctgaa acttttttcc
     9901 agtctgaacg aggcattttt gttccagatg tatcatttgc agccttttga tatctatcag
     9961 cttgaataag agaataaata aagtccgagt cgctcttctt agctctaaaa acccagaaat
    10021 ccccaatcgc tacgcctgta aaagttgcct tgagccaatt ttttaaatat ggacgtaact
    10081 ttccatatag aatatcattt ggtaaaaatt caatcccttt tctattatca aatttctggc
    10141 tcacatcttt attcagtcta ccttctcctg ccacgatatc ttcaaattca acttttggaa
    10201 gtctactgga gtttgtactt ttattaagtc taacaactaa atcaattaac ttacgctctt
    10261 cccaatcttc cgtaaatcct gggaaacgga tttctggtcg tgtatcattg ctcatgtttg
    10321 aacacctcca gcgcaccttg taaccacatt tcgttttcag gtgttgtttg cagggaagag
    10381 atagcttcta aaagttcttt ttctagcact tgctgttctt gtcgtatctt cttgatgtca
    10441 tttccgacat gaaccatatc aattggttct tcttcttcga aagtatctac atagcgtggg
    10501 atatttaagt tatagtcatt ttctttgatt tcatcaaagg ttgccaaatg agcgtatttc
    10561 tcgacatctt ttctttcagc gtaggtttcg aggatttttt gaatgttttc ttccgatagt
    10621 ttattttgat ttttcccttt aacaaattca cgactagcat caatgaataa tacatctcga
    10681 gtttgacgat tcttcttcaa cacaatcacc gtcgttggaa tcgatgtccc aaagaacaag
    10741 ttcgcaggca taccgatcac agcataaata ctgccatctt ctagtaattt ttgacggatg
    10801 accccttctg cagcgccacg gaataaaact ccgtgtggta agacgatggc catggtacct
    10861 gtttccttca aatgatagaa cccatgaaga agaaacgcaa aatctgcttt ggattttggc
    10921 gctaatttcc cataacgatt aaaccgagaa tcatctaaga aagtcgtatc tgctgaccaa
    10981 ttagccgaat aaggtgggtt cattactact gcatcaaatg tgtaaggttc atctgttggc
    11041 cagtccttat tcaacgtatc cccattgcgg agattcattt cttcggcgtc gacaccatgt
    11101 aaaatcaagt tcatttttgc tagattgtat gtcgttgtat ttagttcttg cccatgatat
    11161 ttaacgttat ctgggtgtgt taagtagtta cgtacgttta acatcaatga acctgaaccc
    11221 atcgttggat caaaaacact aaagaatcgt cgttcctttt gatcgagcgt tacgatttgc
    11281 gccatcatat cagaaaccat atgaggtgtg taaaattctc cggctttctt ccctgcttct
    11341 gaagcaaatt gactgatcag aaactcatac gcatccccga tcacgtcccc atcgtgttct
    11401 aaaacatcta catcattcaa tttcttgatg acttcggtaa tcgtcacatt tcgttgttgc
    11461 tcatctgttc ctagtttttt tgattggaga tccacatcat caaataaccc attaaattga
    11521 ttgtaggtag atgccaattg cacaaaggct ttgtttagat cgttcaattg gaacgttgct
    11581 tgttttgctt gatccgctaa gacgttgaaa agatactctg gtgcaattgc atatcccaaa
    11641 atatcaacga ttgtggcaat cagatcttct tttgattctt catctgaaag taattcacga
    11701 tacaacattg tttgtttgct tactgtgtca tattcctcta aggattcatc tgctaaaaga
    11761 accacttgtt ccaacaatcg atcggataaa tatttataga aaatcagtcc caataaataa
    11821 tttttatatt ctgacgcatc cattttacta cggagattat ctgccgcact aaataatttt
    11881 tgatttaatt ctgcacccaa aaagatacac tcctatctct tatttaaagt tgctatatac
    11941 cctcttattt tagcataccc ccttttataa aaagcaaaaa aaaaagaaag aaacttattc
    12001 ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag actgatcgtt
    12061 aacttatttt ttatatttct acatagttat ctttagaacc atttcagata catatgaaac
    12121 aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa atatacaaat
    12181 ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac ttatatatca
    12241 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
    12301 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
    12361 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
    12421 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
    12481 tttttaccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
    12541 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
    12601 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
    12661 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
    12721 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
    12781 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
    12841 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
    12901 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
    12961 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
    13021 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
    13081 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
    13141 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
    13201 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
    13261 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
    13321 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
    13381 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
    13441 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
    13501 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
    13561 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
    13621 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
    13681 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
    13741 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
    13801 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
    13861 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
    13921 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
    13981 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
    14041 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
    14101 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
    14161 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
    14221 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
    14281 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
    14341 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
    14401 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
    14461 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
    14521 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
    14581 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
    14641 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
    14701 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
    14761 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
    14821 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
    14881 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
    14941 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
    15001 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
    15061 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
    15121 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
    15181 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
    15241 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
    15301 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
    15361 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
    15421 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
    15481 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
    15541 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
    15601 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
    15661 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
    15721 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
    15781 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
    15841 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
    15901 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
    15961 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
    16021 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
    16081 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
    16141 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
    16201 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
    16261 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
    16321 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
    16381 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
    16441 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
    16501 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
    16561 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
    16621 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
    16681 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
    16741 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
    16801 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
    16861 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
    16921 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
    16981 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
    17041 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
    17101 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
    17161 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
    17221 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
    17281 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
    17341 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
    17401 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
    17461 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
    17521 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
    17581 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
    17641 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
    17701 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
    17761 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
    17821 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
    17881 tcaatagaat gatttgggct agtattctta aaaattaact agcaaatgtt taaactgatt
    17941 tatttctaat ctacatgtag taagtatttc aattccacca attatcaata aaaataccaa
    18001 acatatccat aataattttt tctttttcat ttcttttata ttgttataac agtgaataac
    18061 ataaaaattc ccgacaatca ttgtaatatt tttttgtcac ctgtattagt atttaattta
    18121 taggaaacta taaaaactcc ctagtgaatt tacacctttg ggagttcctg tttggacggt
    18181 atgcataata catttaaatc aatttaacat taacacctca gcatatggta tctccacata
    18241 tatacggtct ttctttggat agtactcaat atttatgttg atagcacttc cattgtctaa
    18301 tatagcttta gcccggtatg tgttatctcc attttcatga gtaatcgtca catcatcaca
    18361 tgatcgattt aagttgtact gttcttgtaa aatctgtgtt accaatggtt ttgatgactt
    18421 ctctaatcca gatgtacctg tcatttttga aaaaataata cctataatag ccaagacaac
    18481 aatacctata atgacaaact tcttgttcat atttacttta ttcaactttt tatctcctat
    18541 ctccaaaata atttgttctt atccctctta tcagtacatt gacatcccta aaagtacacc
    18601 aaagattgct ccagctaccc ctgctatcat caaaatcgta cccattttct catctttttc
    18661 tcgatataag tagcccataa taacagcaac agcaccaaat acaacaggaa taaatgctaa
    18721 cgatactacc atagaaatcc aaccaataat caaatacagc ttccctgtct cttgtccatg
    18781 tacgatttgt tccttcattt cattaattgt ctgctcagta cttttcttta ctgtgtctgt
    18841 tccggttgtc tcatctgttt cttgtagtgc tccacaaaac tggcaatact tactttcatc
    18901 tgttatttct ttgccacatt ttctacaata catgtcttct cctcctaaaa tcattcaatt
    18961 gcttattagt taactgttta ctttattaaa tacatcagaa aacagctcat tcattggtac
    19021 acttaatatc tcagaaagca tcttcatttg atagggttta ggaatcgttc tccctgtttg
    19081 ccacgaactt acacattgct gtgtaacatt catttttttt gctaactccg tttgagaaag
    19141 tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc tcccctcctg
    19201 ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa acttcaaata
    19261 ccattacaaa tacaacaact tgttgtacta tactgagatg ggggtgtatt aatgaaacag
    19321 aaacaatatg caaaggtcat tagagaaaag cgaaaagctc tcgggtggac acaaaaagaa
    19381 ttagctgaaa aaatcttctc tacacaacag gcagtagcaa gatgggaaaa ttctgtaaca
    19441 gagcctaacc tcgatagctt aacagctctt tctagagccc taggaacccc tgtaagccat
    19501 tttctagaca atgttgtcgt agattatgag gaagagtttt tggctctcta tcgttctctt
    19561 agcactgaag atgcagttcg aacaattgac tatatgaagc tattaaaaag gcaagaaaat
    19621 gaacgcaatc agctattgaa agattgaaaa ataaataacc caccgaactt taacggtggg
    19681 tgtattgaga tgaaataaga ttaagagcta tggataacct gtctagttta atacaattgg
    19741 gtcttttctc ttccttactc tccgtccatc atctcttgtt acacttgtaa attgatacag
    19801 aggaactatc ccagctcctg ataatatcaa ttcttttatt gtttcatcta tccctgcccc
    19861 tttcgctaaa tatattaaaa aactactact tgctttcaca ttgtatagtt tcttaatatc
    19921 cttagaagct cgttcaagag tgaatccttg tttaacaaag tagcctctgt tttttattaa
    19981 agcatttttc acgctgttat ccccaacatt acgtaggcgt tcacaaacag tgttaataag
    20041 ttctttctct gtcattagat atgtcccttg ccacgataag ccctgtaaaa cttcatgtgc
    20101 ttcatctaaa atcaattgtt tttccttgct gagttctgat cgggaatttt gaggaaatac
    20161 tcttttagcc tctttttcgt taaaacatat cttaacccat tcataagaaa aatattgata
    20221 tgtaaaatga attttttcca gaagtggaag gatatcttca ttcaattttt gaatcacaga
    20281 agttgcatta aaaacgtcta atcgatagac attgcttctc ttatgattaa agtttcctaa
    20341 cgctttctct tcctttaacc tatttaggta ttcgagcatc ttcaaaggaa catcttcatc
    20401 atctaatttt tttatcaatc ctataaaagt cataagtgat agtaacttat tacatttatc
    20461 tatgtcataa gttttaccat agtaagaact cattttttta gctaattgtt cccccgacaa
    20521 aatacaagtc acactttcct tactatcatc aaaaaaatta acatcagaaa gcaagacatc
    20581 atagacacgt cgcatttcct ctagataatt ccttattgtc ctgaaaagtt tgtctttcat
    20641 tgattcaaga gtgtcaaaaa gtacttttga ttcctctttc aattttcgaa aatgtgtagg
    20701 atacaagtct aggttctcta atagccaatt aaatgtttct acccgactgt ctttctttaa
    20761 aattagcatc acaactccaa taaaattttc actgatatca caattactgg aatgacaata
    20821 aaaaatggaa tgcccagttt tactggtaaa aatactagca gagggattat gatcgttgtg
    20881 aaacagacat cttaagtttt tacttgactt aagtaattta ttcatatcaa tggttaacaa
    20941 tctttcataa atctcattaa aagacatgtc ggaggaatca aacaggcagt ctttaaacca
    21001 ctgtttcatt tcttctttgt cgtcattttt tatcaactta acaaaatcgg tttcattgac
    21061 tagttcatac tctctccctg aatcactttg acaattttta gaacatgtca tggtaaaatc
    21121 aatcggaagc tcagtaatat ctaacatgtt gtcttcatta attattatca ctgcatctga
    21181 cttatatcct ccaaaaaaca ttctattcga agcatttaca ttgtcatcac acccggggat
    21241 gttctttttc aagtattcgt aaacagcaac cattttacca tagtcagtaa ttgcttcttt
    21301 taatagaaag acaatcttgt atttttctag attttcagag taccgaatac ttttttgtat
    21361 cgcaaacgca gaattatgaa ctaaattgat agataaaacc ttttctatgc ttaagtatct
    21421 actatcatct ttactcaaat ctactacctt ccccttctca tctttaacag tgttatcgac
    21481 atccagaata attactctgg tttccacaaa actctccttg tcattgcctt tattctttgt
    21541 cttgcttgga ataaatgatt gtgatgaatt tagtatctct acaaactcat ctcttgtaac
    21601 ttctctatag ttatctaaac acctatctct tacatcagtt ttttcttttt ttgtaactgg
    21661 cttctctaat aatttttctt gatctacata aattggaata ttaatcattg atgtttccat
    21721 ttgtgtttcc tccttataaa attctgttaa agactaaaac atacagataa taaaaacaaa
    21781 ctctttatta ctatatgttt tagtcttatt tgtttactca acctttatac cgttccatga
    21841 aactatcaat atcagatttt ttaaatctaa tgaccgtatc aatttttata acaggtaacc
    21901 cgtattgaga aacatatttt ctaatcgttc ctactgatac acctagatat tcacttgcct
    21961 gctgatagtc catatactct agagtgactc cagaaataat gtcttctagc ataggcttta
    22021 gcactgtaat tatctctttc acttgttgtt caacttgtat agaaacaatg ctatcaatga
    22081 tttgtagaga catatttccc cctcctttcg tgattgcttg aacaaatttt ctaagaatta
    22141 tcttcgtatt ttttgctcaa aataaaagta ttttgttatt aaccgattaa tcaaacaaaa
    22201 aatgattacc agttcatcga catacacata atacctcgat aaaaaagggg tgtcaacagc
    22261 tttgtaaaat ttatttacaa cacccccttt tttgaggtat actaatttta ggaggtgtgg
    22321 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
    22381 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
    22441 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
    22501 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
    22561 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
    22621 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
    22681 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
    22741 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
    22801 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
    22861 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
    22921 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
    22981 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
    23041 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
    23101 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
    23161 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
    23221 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
    23281 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
    23341 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
    23401 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
    23461 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
    23521 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
    23581 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
    23641 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
    23701 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
    23761 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
    23821 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
    23881 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
    23941 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
    24001 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
    24061 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
    24121 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
    24181 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
    24241 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
    24301 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
    24361 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
    24421 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
    24481 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
    24541 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
    24601 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
    24661 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
    24721 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
    24781 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
    24841 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
    24901 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
    24961 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
    25021 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
    25081 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
    25141 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
    25201 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
    25261 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
    25321 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
    25381 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
    25441 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
    25501 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
    25561 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
    25621 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
    25681 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
    25741 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
    25801 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
    25861 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
    25921 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
    25981 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
    26041 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
    26101 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
    26161 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
    26221 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
    26281 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
    26341 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
    26401 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
    26461 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
    26521 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
    26581 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
    26641 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
    26701 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
    26761 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
    26821 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
    26881 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
    26941 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
    27001 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
    27061 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
    27121 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
    27181 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
    27241 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
    27301 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
    27361 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
    27421 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
    27481 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
    27541 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
    27601 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
    27661 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
    27721 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
    27781 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
    27841 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
    27901 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
    27961 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
    28021 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
    28081 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
    28141 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
    28201 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
    28261 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
    28321 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
    28381 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
    28441 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
    28501 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
    28561 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
    28621 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
    28681 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
    28741 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
    28801 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
    28861 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
    28921 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
    28981 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
    29041 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
    29101 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
    29161 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
    29221 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
    29281 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
    29341 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
    29401 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
    29461 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
    29521 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
    29581 tatcttttat ttattattaa acaattttga tttctaaatt agaataggta taaaaaagat
    29641 gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct tattttgcat
    29701 cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt
    29761 tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca cctttacgag
    29821 tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca tcttctaatg
    29881 gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca ccttcatcat
    29941 tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag
    30001 ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga
    30061 ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca gaatttgaag
    30121 taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta gagtacaagc
    30181 ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt tctttgattt
    30241 ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct tcttccaaag
    30301 attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt ccttcatctt
    30361 tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa gcgccgactt
    30421 tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg
    30481 ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc tttttcatat
    30541 cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat
    30601 tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg tcactgttag
    30661 attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc
    30721 gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa
    30781 tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat
    30841 tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg
    30901 ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat
    30961 agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt
    31021 tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag catacggttt
    31081 gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa
    31141 gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg
    31201 gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct
    31261 caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac
    31321 cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat
    31381 acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg
    31441 catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt
    31501 cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg
    31561 ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc
    31621 ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga
    31681 tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt
    31741 ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat
    31801 ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt tgtgaaggat
    31861 atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac
    31921 gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc
    31981 ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta
    32041 attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg
    32101 gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga
    32161 cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt
    32221 ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat
    32281 tcacatcttt ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa
    32341 aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca
    32401 gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact
    32461 cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt
    32521 tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt
    32581 ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt
    32641 gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca
    32701 tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct
    32761 tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg
    32821 accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct
    32881 tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca
    32941 acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg
    33001 cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag
    33061 ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg ataagacttg
    33121 gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt
    33181 catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc
    33241 aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac taacgccatg
    33301 ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt
    33361 gtacaatttt cttacctcct tgaagatctt acaggtgctt cattttggta tctcatcgat
    33421 aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg actaatgaaa
    33481 ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca
    33541 tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta
    33601 tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat gacgaatgca
    33661 ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga ctcacgaaca
    33721 ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc
    33781 aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat
    33841 gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa gtctcttccg
    33901 ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca
    33961 ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa gatcgcaaga
    34021 aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc
    34081 acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc
    34141 gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct
    34201 gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct
    34261 aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa accataaagg
    34321 taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc
    34381 gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc
    34441 ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact
    34501 ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg
    34561 tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg
    34621 gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc
    34681 aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca
    34741 tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc tttcattgta
    34801 tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat
    34861 gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg gaacagagcg
    34921 cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact
    34981 tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg
    35041 aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat
    35101 gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc
    35161 gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgt
    35221 gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg
    35281 caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt cttcattttg
    35341 ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc
    35401 atataataag tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag
    35461 atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa
    35521 aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt
    35581 taataactta aacatggcac cacttccata actttttatg ataattacta taaaaactat
    35641 ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat
    35701 ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct
    35761 attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa
    35821 tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg
    35881 ggttagcttc ccttggaaat ctgtttaaag actatcatca tgttgcttta ggcaatctaa
    35941 ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg
    36001 aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga
    36061 tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca
    36121 aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc
    36181 accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt
    36241 acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt attggaaaaa
    36301 ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg
    36361 tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac
    36421 ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg
    36481 tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac
    36541 gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct
    36601 ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg
    36661 tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc
    36721 ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta
    36781 aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat
    36841 aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct tattaccgca
    36901 gatgcaagct tttgaacacc gtttaccaag aggtcgtaa
//
LOCUS       DAFFQY010000020        35949 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFFQY010000020 DAFFQY010000000
VERSION     DAFFQY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.020"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(5..1282)
                     /locus_tag="LRB02_000915"
     CDS             complement(5..1282)
                     /locus_tag="LRB02_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659138.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="LRB02_000916"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="LRB02_000916"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL4659139.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="LRB02_000917"
     CDS             complement(2204..3148)
                     /locus_tag="LRB02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL4659140.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="LRB02_000918"
     CDS             complement(3150..3836)
                     /locus_tag="LRB02_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL4659141.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="LRB02_000919"
     CDS             4310..4630
                     /locus_tag="LRB02_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL4659142.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="LRB02_000920"
     CDS             4784..5689
                     /locus_tag="LRB02_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659143.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="LRB02_000921"
     CDS             5686..6459
                     /locus_tag="LRB02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL4659144.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="LRB02_000922"
     CDS             complement(6640..8064)
                     /locus_tag="LRB02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659145.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="LRB02_000923"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="LRB02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL4659146.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="LRB02_000924"
     CDS             complement(8786..10768)
                     /locus_tag="LRB02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4659147.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="LRB02_000925"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="LRB02_000925"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL4659148.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="LRB02_000926"
     CDS             complement(11681..12742)
                     /locus_tag="LRB02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL4659149.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13519)
                     /locus_tag="LRB02_000927"
     CDS             complement(12953..13519)
                     /locus_tag="LRB02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4659150.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="LRB02_000928"
     CDS             complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="LRB02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL4659151.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="LRB02_000929"
     CDS             complement(15307..16428)
                     /locus_tag="LRB02_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4659152.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="LRB02_000930"
     CDS             16554..17495
                     /locus_tag="LRB02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL4659153.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="LRB02_000931"
     CDS             complement(17645..18541)
                     /locus_tag="LRB02_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL4659154.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="LRB02_000932"
     CDS             18810..20150
                     /locus_tag="LRB02_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659155.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="LRB02_000933"
     CDS             20368..20811
                     /locus_tag="LRB02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL4659156.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="LRB02_000934"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="LRB02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL4659157.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="LRB02_000935"
     CDS             complement(23136..24359)
                     /locus_tag="LRB02_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL4659158.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="LRB02_000936"
     CDS             complement(24390..25229)
                     /locus_tag="LRB02_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL4659159.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="LRB02_000937"
     CDS             complement(25246..25926)
                     /locus_tag="LRB02_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659160.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25959..27002)
                     /locus_tag="LRB02_000938"
     CDS             complement(25959..27002)
                     /locus_tag="LRB02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659161.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKKRFR"
     misc_binding    complement(27058..27296)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(27339..27572)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27649..28320)
                     /locus_tag="LRB02_000939"
     CDS             complement(27649..28320)
                     /locus_tag="LRB02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4659162.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28407..31085)
                     /gene="mgtA"
                     /locus_tag="LRB02_000940"
     CDS             complement(28407..31085)
                     /gene="mgtA"
                     /locus_tag="LRB02_000940"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL4659163.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31744..32181
                     /gene="ndk"
                     /locus_tag="LRB02_000941"
     CDS             31744..32181
                     /gene="ndk"
                     /locus_tag="LRB02_000941"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL4659164.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32203..33069)
                     /locus_tag="LRB02_000942"
     CDS             complement(32203..33069)
                     /locus_tag="LRB02_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4659165.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33229..35322)
                     /locus_tag="LRB02_000943"
     CDS             complement(33229..35322)
                     /locus_tag="LRB02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4659166.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10411 a         7547 c         6346 g        11645 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatgacac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttttt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg ttttttttgt
    25981 aaaatattta tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg
    26041 gtttctccag ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca
    26101 ttgccatata aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt
    26161 tcgttcgtct gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg
    26221 gcaaaagcag aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga
    26281 ataaaatctt tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt
    26341 tcaacgattc ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc
    26401 atctcatgag tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat
    26461 gcaaggatct gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat
    26521 accttaggtt cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac
    26581 agttgtttag gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc
    26641 actttttgac gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg
    26701 tcaaaaatag ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt
    26761 ctttcttttc gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact
    26821 tctcctgctg ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca
    26881 cctgagtaac cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct
    26941 actgcctgta tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc
    27001 attaaaattc ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt
    27061 gaggacgctt ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc
    27121 ttcaagtacg atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat
    27181 gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct
    27241 accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt
    27301 caaaaaaaaa gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt
    27361 accacctttt ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc
    27421 gttcgtttta acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa
    27481 ggccattttc agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt
    27541 tccaaactta ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg
    27601 aggaaaactg aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt
    27661 ttttttattt aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa
    27721 tttttccttt tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac
    27781 gcctaatcct gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc
    27841 tgaactagct tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt
    27901 gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg
    27961 atccaagata tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg
    28021 acgttctttt aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct
    28081 tgtcagctgt tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg
    28141 gctacctgct gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc
    28201 gtcaacgatt tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc
    28261 acgatacgtt ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat
    28321 ctatacatta atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa
    28381 cagaagcaaa gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt
    28441 ttcttcacag ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag
    28501 taattgccag gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca
    28561 gaaccgacaa taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg
    28621 ataaatggta ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat
    28681 tcgacaaacc atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat
    28741 gtatggaaac ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta
    28801 gtaatatcaa atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat
    28861 gcattccatt tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa
    28921 atgcaggaaa catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa
    28981 aatggcagga acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct
    29041 gtagccttga tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt
    29101 tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc
    29161 acagctgtat ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata
    29221 ccatctccta aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct
    29281 ttttgctgag gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca
    29341 tgatcactca tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact
    29401 tgacggcaca ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca
    29461 ccatgttctt tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa
    29521 gcaagataac caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg
    29581 cttggattgg tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt
    29641 acttttgcaa gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga
    29701 tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt
    29761 ttaccagctg gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt
    29821 tcatccactt tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg
    29881 atagccacat ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg
    29941 cgcaaaacac gatcatcttc atttccttca caatccatat gatattcaag aatgatttta
    30001 tcttgtgtca atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg
    30061 atggcattca aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc
    30121 aaattcgtcg taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt
    30181 ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct
    30241 ggagccatca atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca
    30301 aagcttgatt gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca
    30361 tttccaacag aaacaatgat tccttccgca cttccactga tgacattact tcccataaag
    30421 acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt
    30481 tctactggat agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac
    30541 aataaacgca tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag
    30601 acgatttctt ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact
    30661 ttcacaagtg atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc
    30721 attgtcccgc tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct
    30781 ccaggagctg ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact
    30841 aatgtaaatg gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca
    30901 tatgaaatcg cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat
    30961 ccatttgctg atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct
    31021 gcatagattt tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa
    31081 gacagctgtt ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact
    31141 gaaaggggct gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc
    31201 gaatgactgt catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc
    31261 caatcatttt tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga
    31321 tggggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag
    31381 catattaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa
    31441 gcagatacct gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc
    31501 cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa
    31561 caaatcttat tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa
    31621 ttttccatgt ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt
    31681 tttatttgtc tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct
    31741 gttatggaaa gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga
    31801 agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact
    31861 atgactcctg aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat
    31921 gaactgatcg attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa
    31981 gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt
    32041 acgatccgag gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc
    32101 cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg
    32161 tctgaaaaag cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc
    32221 aatggccagc agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag
    32281 cactgtgaag aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa
    32341 tctttgataa taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc
    32401 taagctccgt ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat
    32461 ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg
    32521 tgctaatcgt cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact
    32581 gaatatccaa ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg
    32641 catcaagtat tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga
    32701 tacttctgtt ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt
    32761 cttaaaatgc ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac
    32821 ccgatctagc attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat
    32881 ccattcatat tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa
    32941 tgacattttt ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc
    33001 ttccgctaat tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc
    33061 atcatacata atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg
    33121 ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac
    33181 cttcaagcat tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt
    33241 tttttgctgt tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc
    33301 attttttgaa aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg
    33361 ttccgcttca tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac
    33421 ataaaaccgc aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac
    33481 tttttcctga tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc
    33541 tcctgtctga ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt
    33601 cccatttcga aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg
    33661 aagataatct ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc
    33721 tccaccgtat tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg
    33781 cactcgttgc atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc
    33841 gcttgctaaa tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa
    33901 gattgaattt tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg
    33961 atccagataa tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga
    34021 tggaaagtcg aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa
    34081 tttcgtgtaa aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc
    34141 tttatctaat atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa
    34201 gaaattgtca taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt
    34261 cccatggaaa acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg
    34321 aaagacattg tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc
    34381 agtttccaac atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt
    34441 gtcaaagctg atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg
    34501 tccgtctacc ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac
    34561 atttttccct ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt
    34621 ataatctaaa acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat
    34681 tcctgttttg attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata
    34741 tgaacggtca aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt
    34801 tcctgaaaaa ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg
    34861 gttgcttttg ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc
    34921 gatatccagc cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa
    34981 tccttgcgac actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc
    35041 ccgataaaaa ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag
    35101 aatagctaac gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt
    35161 agtcgcaatt gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga
    35221 aaaatctgca tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac
    35281 gattgctaaa cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta
    35341 ttcacatttt ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct
    35401 taagattttt ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct
    35461 gcgatgtaag cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca
    35521 cggtattcaa aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct
    35581 tgtcgcagct ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg
    35641 tgttctcctg ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt
    35701 ttttgaaatt gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    35761 ggtgttcaat cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc
    35821 ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    35881 taaggagcta ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac
    35941 ctcttgata
//
LOCUS       DAFFQY010000021        35657 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFFQY010000021 DAFFQY010000000
VERSION     DAFFQY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.021"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..2198)
                     /locus_tag="LRB02_000944"
     CDS             complement(<1..2198)
                     /locus_tag="LRB02_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659167.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="LRB02_000945"
     CDS             complement(2185..2403)
                     /locus_tag="LRB02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659168.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="LRB02_000946"
     CDS             complement(2405..3448)
                     /locus_tag="LRB02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4659169.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="LRB02_000947"
     CDS             complement(3603..3929)
                     /locus_tag="LRB02_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659170.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="LRB02_000948"
     CDS             complement(3937..4140)
                     /locus_tag="LRB02_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659171.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="LRB02_000949"
     CDS             4215..4865
                     /locus_tag="LRB02_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659172.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="LRB02_000950"
     CDS             4920..6086
                     /locus_tag="LRB02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4659173.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="LRB02_000951"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="LRB02_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL4659174.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="LRB02_000952"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="LRB02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL4659175.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="LRB02_000953"
     CDS             complement(7545..8012)
                     /locus_tag="LRB02_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL4659176.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="LRB02_000954"
     CDS             complement(8132..9670)
                     /locus_tag="LRB02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL4659177.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="LRB02_000955"
     CDS             9750..10436
                     /locus_tag="LRB02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL4659178.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="LRB02_000956"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="LRB02_000956"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4659179.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="LRB02_000957"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="LRB02_000957"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL4659180.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="LRB02_000958"
     CDS             complement(11922..13052)
                     /locus_tag="LRB02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4659181.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="LRB02_000959"
     CDS             complement(13052..15235)
                     /locus_tag="LRB02_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL4659182.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="LRB02_000960"
     CDS             complement(15232..16257)
                     /locus_tag="LRB02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4659183.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="LRB02_000961"
     CDS             complement(16302..17129)
                     /locus_tag="LRB02_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL4659184.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="LRB02_000962"
     CDS             complement(17126..18193)
                     /locus_tag="LRB02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL4659185.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="LRB02_000963"
     CDS             complement(18219..19055)
                     /locus_tag="LRB02_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4659186.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="LRB02_000964"
     CDS             complement(19052..19921)
                     /locus_tag="LRB02_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4659187.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="LRB02_000965"
     CDS             complement(19991..21274)
                     /locus_tag="LRB02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL4659188.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="LRB02_000966"
     CDS             complement(21288..22973)
                     /locus_tag="LRB02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL4659189.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="LRB02_000967"
     CDS             23248..24273
                     /locus_tag="LRB02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659190.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28542)
                     /gene="rpoC"
                     /locus_tag="LRB02_000968"
     CDS             complement(24868..28542)
                     /gene="rpoC"
                     /locus_tag="LRB02_000968"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL4659191.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="LRB02_000969"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="LRB02_000969"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL4659192.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="LRB02_000970"
     CDS             32945..33913
                     /locus_tag="LRB02_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4659193.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="LRB02_000971"
     CDS             33931..34836
                     /locus_tag="LRB02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659194.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="LRB02_000972"
     CDS             34833..35639
                     /locus_tag="LRB02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659195.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10106 a         7532 c         6435 g        11584 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctataata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctccctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tgatttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaacat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggac
//
LOCUS       DAFFQY010000022        35594 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFFQY010000022 DAFFQY010000000
VERSION     DAFFQY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.022"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            2..400
                     /locus_tag="LRB02_000973"
     CDS             2..400
                     /locus_tag="LRB02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL4659196.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LRB02_000974"
     CDS             384..1079
                     /locus_tag="LRB02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL4659197.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="LRB02_000975"
     CDS             complement(1663..2550)
                     /locus_tag="LRB02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL4659198.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="LRB02_000976"
     CDS             2735..3574
                     /locus_tag="LRB02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4659199.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="LRB02_000977"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="LRB02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL4659200.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="LRB02_000978"
     CDS             5042..5398
                     /locus_tag="LRB02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659201.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="LRB02_000979"
     CDS             5401..6558
                     /locus_tag="LRB02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659202.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="LRB02_000980"
     CDS             complement(6584..7024)
                     /locus_tag="LRB02_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL4659203.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="LRB02_000981"
     CDS             7184..7690
                     /locus_tag="LRB02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4659204.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="LRB02_000982"
     CDS             7858..9771
                     /locus_tag="LRB02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL4659205.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="LRB02_000983"
     CDS             9776..10777
                     /locus_tag="LRB02_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4659206.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="LRB02_000984"
     CDS             complement(10979..11185)
                     /locus_tag="LRB02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL4659207.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="LRB02_000985"
     CDS             complement(11831..12679)
                     /locus_tag="LRB02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4659208.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12678..13745
                     /gene="gnd"
                     /locus_tag="LRB02_000986"
     CDS             12678..13745
                     /gene="gnd"
                     /locus_tag="LRB02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL4659209.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="LRB02_000987"
     CDS             13747..14748
                     /locus_tag="LRB02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL4659210.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="LRB02_000988"
     CDS             14745..15029
                     /locus_tag="LRB02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL4659211.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="LRB02_000989"
     CDS             15062..15859
                     /locus_tag="LRB02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4659212.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="LRB02_000990"
     CDS             15873..16706
                     /locus_tag="LRB02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659213.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="LRB02_000991"
     CDS             16724..17581
                     /locus_tag="LRB02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4659214.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="LRB02_000992"
     CDS             17603..18112
                     /locus_tag="LRB02_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4659215.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="LRB02_000993"
     CDS             18149..18550
                     /locus_tag="LRB02_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659216.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="LRB02_000994"
     CDS             18662..19015
                     /locus_tag="LRB02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659217.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="LRB02_000995"
     CDS             19049..19492
                     /locus_tag="LRB02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL4659218.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="LRB02_000996"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="LRB02_000996"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL4659219.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="LRB02_000997"
     CDS             20587..21300
                     /locus_tag="LRB02_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4659220.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21629..22930
                     /locus_tag="LRB02_000998"
     CDS             21629..22930
                     /locus_tag="LRB02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4659221.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(23008..23157)
                     /locus_tag="LRB02_000999"
     CDS             complement(23008..23157)
                     /locus_tag="LRB02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659222.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            23553..23753
                     /locus_tag="LRB02_001000"
     CDS             23553..23753
                     /locus_tag="LRB02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4659223.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24321..24884
                     /locus_tag="LRB02_001001"
     CDS             24321..24884
                     /locus_tag="LRB02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659224.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(24945..25289)
                     /locus_tag="LRB02_001002"
     CDS             complement(24945..25289)
                     /locus_tag="LRB02_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659225.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25386..25631)
                     /locus_tag="LRB02_001003"
     CDS             complement(25386..25631)
                     /locus_tag="LRB02_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659226.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25701..25955)
                     /locus_tag="LRB02_001004"
     CDS             complement(25701..25955)
                     /locus_tag="LRB02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659227.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26119..26442)
                     /locus_tag="LRB02_001005"
     CDS             complement(26119..26442)
                     /locus_tag="LRB02_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659228.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            26629..26702
                     /locus_tag="LRB02_001006"
     tRNA            26629..26702
                     /locus_tag="LRB02_001006"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26663..26665,aa:Ile,seq:cat)
     gene            complement(27087..27584)
                     /locus_tag="LRB02_001007"
     CDS             complement(27087..27584)
                     /locus_tag="LRB02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4659229.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(27624..28433)
                     /locus_tag="LRB02_001008"
     CDS             complement(27624..28433)
                     /locus_tag="LRB02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4659230.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            28767..29624
                     /locus_tag="LRB02_001009"
     CDS             28767..29624
                     /locus_tag="LRB02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL4659231.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            29617..30519
                     /locus_tag="LRB02_001010"
     CDS             29617..30519
                     /locus_tag="LRB02_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL4659232.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            30552..30902
                     /locus_tag="LRB02_001011"
     CDS             30552..30902
                     /locus_tag="LRB02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4659233.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            30951..31873
                     /locus_tag="LRB02_001012"
                     /pseudo
     CDS             30951..31873
                     /locus_tag="LRB02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            31951..32844
                     /locus_tag="LRB02_001013"
     CDS             31951..32844
                     /locus_tag="LRB02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4659234.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            32882..33445
                     /gene="amaP"
                     /locus_tag="LRB02_001014"
     CDS             32882..33445
                     /gene="amaP"
                     /locus_tag="LRB02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL4659235.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            33459..33647
                     /locus_tag="LRB02_001015"
     CDS             33459..33647
                     /locus_tag="LRB02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL4659236.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            33660..34562
                     /locus_tag="LRB02_001016"
     CDS             33660..34562
                     /locus_tag="LRB02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4659237.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            34594..34836
                     /locus_tag="LRB02_001017"
     CDS             34594..34836
                     /locus_tag="LRB02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4659238.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(35092..35463)
                     /locus_tag="LRB02_001018"
     CDS             complement(35092..35463)
                     /locus_tag="LRB02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659239.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        12347 a         5718 c         7129 g        10400 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc cacggctcta taatttctag gactgataga aaatcattct
    21541 atcggtccct tttgagcatg aaaaaacaaa acatacagaa gctccgttag aattgaatca
    21601 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca aactactact
    21661 tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca ttaagaatat
    21721 caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc ctttgtgtaa
    21781 acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc tcattcaact
    21841 taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct accactgtcg
    21901 tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact gctttatcgc
    21961 taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa tgacgacaat
    22021 tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt ctctgatccc
    22081 catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg aatttcgttc
    22141 actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata ctggaaaatt
    22201 actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta tgcaatttga
    22261 acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt cttatgcctc
    22321 tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc acattgtcaa
    22381 acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct ttgatcgaaa
    22441 cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc ttctgatcaa
    22501 acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg gttgggcgta
    22561 tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat tacgaatcgc
    22621 ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg acactttctt
    22681 taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta taaaaaaagc
    22741 ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt caaatgccaa
    22801 aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg gctttcgaag
    22861 ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct atgaatcaaa
    22921 aaacatctaa agtagaaaag gaattctacc ttagatgttc ttatctcact ctatcagtcc
    22981 tatttgacaa agagccgttt ccactcctta tgcagatttt tttaattctt gcactgcttg
    23041 ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag
    23101 attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga atgtcatagc
    23161 cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca
    23221 tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt
    23281 tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt tattcaactt
    23341 tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc attatccttg
    23401 tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg taatttgagc
    23461 gatatatcta catagataaa actccttttg cctatcaaat atttattgca acgaaaaaac
    23521 gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta aaatggttca
    23581 acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta tttgcacatt
    23641 tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg
    23701 atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct taataaaaat
    23761 tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa aattcattgg
    23821 tcgttgaatg ggctgaatag tagtgtataa aacaattttg tggagcgtga agcttttatg
    23881 gtagaggaaa atacgaataa agtgatgttg ataaaaagat tcctatcttg aatatgaatc
    23941 gagatgtctt gaaaatgaat gagtttgacc aatcattatc ctatcgaaat ttacagtttc
    24001 gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa tatgttctag gaagagggcg
    24061 gcacatctaa atttttaagt aacggacagt tcaaatatca agcattgaaa gaggctatcc
    24121 tactgtttga aggaatacga atatataaaa acgacaaaga ggaaaaagta gttcattaaa
    24181 tcccttcaag tgatgaaaga atgagatcta ataaaacctg atctttcatc tgttttttta
    24241 ataacgtaat aattttttta ttttttgtgc tttcgccgtt attgtagagt gatttagcat
    24301 tcttattttt aaaaataact gtgttatata ttaaaaaagg cataatagtt ataagctttt
    24361 ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa agcgttatta
    24421 ttttagtcta ttttcagggg gggaacctta tgataaaaaa actattttta ctatcaatat
    24481 taacgctagc aatggcaggt tgttctgact ctggcttagc aactgaactg atcacaaaat
    24541 caactgaagc cacgacagag gagttaaaat gtggaaacga tcacagtgac tacagaggct
    24601 taaatcgacg atgcatggga atattaggca gaagcaattg taagcatcga tgtagtaatc
    24661 gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt
    24721 gctgtgcatc gtatacagac gtcgaagatg cggtactttt aaaccacgtc aggagatgtt
    24781 catttatgtc tagggaagtt ggcgatgaac caaaactaga gtacgctttt agttttgcac
    24841 gtgaggatga agataaccct aaaacttacg acattttagc ttgatctaag atggttaaag
    24901 ctttttaatg aagtaagagg agatagaagg cttttctagt tatattaggt aagataaatt
    24961 cctttttgct ccaaatttgc taaacattct gctagataaa cttcatcgtg ttccggataa
    25021 atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga
    25081 tacataggat ttttccactc tggtgctggg ctgtaaccac gtttttccat ttcatccatg
    25141 acaagctgat gatattggaa taatctataa ggagaatagt gaaaaacata atccacagta
    25201 gcatgtttct ttccccagcc atttcctctt aatgcacagc attcccgatg ttgtcctaaa
    25261 agctgttgac gaggaagctt tgaaatcaat ttttcatgcc ataatcacat agaaatgtct
    25321 cctttatata tagattgtta accttagacg ttaaatgaaa aaaccagcac caaggctgga
    25381 aaaaatcact tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc aacggaaata
    25441 tcgtaaaagt cagcgatata gattaattca atgatgcttg gaatttcttc tccttcttcg
    25501 tacttacgat atttgaataa aggaatactt aacgcgctag aaacagctac ttgagataaa
    25561 gatcggccag ttcttaagaa attcagacga caacgtaaag tatccagtaa aatagcttct
    25621 tcgatactca tttaatcacc tttcattata gtagaatagt tataatgact atagaaagct
    25681 acacatgcaa tcaatagtac tcaatgtttt ttatttaaac gtatcaaata aagaatgacg
    25741 atcaataaag acaaaaccag tcctgataat tcttctataa aaacactatc gggttcaaag
    25801 tagttgtaat taaaaacttt ccacataaag tagatagccg gaagagtcaa aatagctaaa
    25861 taaaaccgtt ctttgttcca attcataaga taaacaatac ttgcgaccag tgcgaccgca
    25921 ggcacccaaa acaaaactct caccaacgta atcatgtaac ggactcctca acgattcata
    25981 taataaaaga taacatgtta ttgcatacat cacaaatgaa atgctaggaa aataaaagaa
    26041 gaatagatca atgaaaaatg aaaaagcaga acagttttct gctgataagc aggaactgtt
    26101 ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga ttgatatcta agtaattcaa
    26161 tacatataaa aagaggacga tcgcattcac taaaaaggaa agaatgatga tcccatagcg
    26221 aatggaaaca tatttaacaa atacttttgt ttgaatacgg gcatatcttt ttcctttata
    26281 gctgagcatc atgacaagaa gcaaaagggt aatcacacct tgaaaaatca ttccaataaa
    26341 cagaattcct gaggtttgat aatttaattg gagaaaataa gctgctgccg caactaaatc
    26401 gaataaaatc gcaataaatg gcaaaataat cactcctaac attgagaata aataaaagaa
    26461 taacattttt tctaaaataa gcaaataaaa attaaagtaa caaaatagtt atgacacaag
    26521 taaaagaaat aagaataaca atattatttt acttatattt tgaaacttgt gagagtttgt
    26581 cattcgtgct atagttgaaa agtaacaaaa ataaatttta tttatgatgt acctttagct
    26641 cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc ctacaaggta
    26701 cataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca
    26761 gcttcttatg tttttacttg gctgttctta ttactgcaga tgcaagcttt tgaataccgt
    26821 ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagcttaac aaacggaaaa
    26881 atagttcctc atattttgcc gtttgttaag ctaataccga gggtcagctg attgaacacc
    26941 atttaagaaa gaggtcgtga gcctgccgtt tcactctgtg tcataaaaaa gccaatctac
    27001 tttgatagaa aaagcacgta ggactaaaac agttctgcgt gcttttttta ttgtgtgtta
    27061 aatatcgtaa atgaagatag atatttttat gattcgacga tagagacgtt gaaatctttc
    27121 aacagctcta aagcggattc tccgtgtttt ctactggaga ctaacattgg atcaacaaaa
    27181 aagtctgctt caggaaaagc actctgcagg cctatcagat tagagagtac acacaagttt
    27241 gtttcaatcc cgactacttc gatctctttt tgttcttctt tgctttcaaa taaagtttcc
    27301 tggatttcag ctaaagtttc aggtggaatc gtaaagtaat actttttaat cactcgttca
    27361 ttttcttgtg gagaaagaag agggatcaat tgcaaatctt ctcgttcttc ttctttgtcc
    27421 ttcagttcaa ctggtatatc ccgtgtgaag agaatatatt cattattttg gcgtgcttta
    27481 gctaatcttt gagcaatacg ttctgccaat tgatctgact ctggaatata aaattcgctg
    27541 tttggatcaa gaatatgatt ttgcatatca ataataacta acattttctt tcctcctaca
    27601 ttcatatcgt cattttattc tttttaatag aagtttttta aaatataagc aacaccagct
    27661 tcttcattcg tcagtgttac ttggtttgca atttctttta cttgtcgctg tgcattcccc
    27721 atcgcaatac tcaacccgct gtaattcaac atatcaatgt cgttttcttg atcgccaaaa
    27781 gccatgatgt cactcgattc gattccaagg taatccgcca gaaaaacaag tgaatgagct
    27841 ttagtgatac ctgtaggcgt gatttctaag aaatgcggat cagagaatac tgattggata
    27901 ccatctgctt gcagatctct ttgtagcttc tgtagttttt cttcactctt atctgttaat
    27961 gctaatttaa gatagctaag agaaggatct tccaacatat ttaaaatttg atcaaaagaa
    28021 tggttctgta aattgaaatg ttcataataa gggtcatctt tgactagatt tgattcatcg
    28081 aatgtcagct gatcatcttg ggtttctcgg ctagcaatga tggatatttt cgcttcttga
    28141 gcatagaaga aggcttttct cagtgtatcg ctatctattt ttgaacgtct gagaactttc
    28201 gccatttttc catccttcat ttcagcaatc agtgaaccat tggaagaaat caaatagcca
    28261 ccgattccta attcggaaaa aatatcttta gcagaaatgc tgttccttcc ggtagcgacg
    28321 gttacgattc cgccattttc atcaaatttc cgaagtgctt ggatattttc ttctggtatc
    28381 tttccatttc gatcaagcaa ggtaccgtct aaatcaatcg ctgctagttt cattttatct
    28441 tcctactttc tttttacata tctattttcc tgttacctta agattaaatg agaatttaaa
    28501 aaaatgaaac taacttgctt tgtcattatg aattttttca aaaaacattc ttaagaaaaa
    28561 ggtggctata tatagtagtt tttggtcaat acctttgaat aaaaagccat gaaaaacgaa
    28621 tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt taaaatattc aatgaaaaca
    28681 aagcatatat tttgctttaa aaccaaatga ggaatattct agatgtagaa caggttagaa
    28741 aaagattaga aaaaggagtg attttgatgg agaagagata cggcgtagtt cctgtggtaa
    28801 ctgcttcaga cgaaaattat gctccttact tgagcgtgat gatcgcaact gcattagaaa
    28861 attgcaataa aacaagacgc ataaaatttt atgtgattga cgatggtcta tcagaatata
    28921 gtaaagaagg attggaagaa acggtaaata aatattcaag taatgcaagt atccaattcc
    28981 tgacagtgga aaaagatatc tacgaagact tcctagtcag tgaccatatc acaactaccg
    29041 cttacttaag aatttcttta ccaaacttat tagctaaaga ggattataag aaagtccttt
    29101 acttagattc agatgtgtta gtactagacg atatcgtaaa actatatgat gagccgttaa
    29161 atggaaaaac aatcggcgct attatcgatc caggccaagt gaaagctttg gaaagattag
    29221 gaatcgattc cgatgacttg tacttcaatt caggagtgat ggtgattgac attgatcaat
    29281 ggaataaaaa agaaattaca gaaaaaacta tccattacct cagtgaaaat ggcgatcgaa
    29341 tcatctatca cgaccaagat gctttgaatg ctgtacttta tgaagattgg gaacaacttc
    29401 atccaaaatg gaacatgcag acttcgctga tttttgaacg tcatccagca ccaaatgaaa
    29461 aatatgaacg tcagtataaa gaaggaaatg aaaaaccttc gatcgttcac tttacaggac
    29521 atgataaacc ttggaatacg ttgaaagatc atccgtatac caatttatac ttaaaaaaat
    29581 tagcacacag tactctaacg aaagtaggcg agatcaatga ataaaaaaga aatagcagta
    29641 gtcgcaagtt gtaacacaaa attcgtacca catttagctg cattatttgt ctcagtatta
    29701 gataattgca acccgtctaa atttgttcgg ttctatgtga ttgacgatga tatcgatttt
    29761 gaaagtaaac agcttttacg cttctcagtg aaaaacgcac gaatgaactc agatgtggaa
    29821 ttcttgaaaa tcaataaaga attctttaca aatgtggtaa tcagcgatcg tattccagaa
    29881 acagcttatt atcgtattgc gattccggaa ttgttccgcg gcacagaagt tgaacgtatc
    29941 ttatatatgg attgcgacat gatcgcttta caagatatct ctaaattatg gcgtcttgac
    30001 ttcggtgatt caatcgtagc agctgttgaa gacgctgggt tccatcaacg actggaaaaa
    30061 atgaaaattt cagctaaatc aatgcgttat ttcaactcag gtttgatgct gatcaatgtg
    30121 aaaaaatggt tagatgaaaa tattacgcaa aaagtattag atttcatcga acataatcca
    30181 gaaaaattac gattccatga ccaagatgca ttaaatgcta tcttacatga ccgttggtta
    30241 ccacttcatc cgcgttggaa tgcacaaggc tatatcatgg caaaagcaaa aaaacatcca
    30301 acagcagcag gtgaacgtga atacgaagaa actcgcaaca atccttacat tatccatttt
    30361 tcaggacatg tgaaaccgtg gagcaaggat tttgaagggc caacgaagaa atattacgaa
    30421 aaatatgcag ggatgacggc tttccgttgt gtagctaaat tccctaaata tccaaaatat
    30481 gcaaaagttc aaccaaaaac agatgtagtt gttaaataat agaagacaac aactgaagag
    30541 ggggagtagg aatggaaatt acaatgtcta aaaatgcagt agagacgtta attgaaaagg
    30601 ttggggaaaa tacaaaaatc gcactcgcgc ttatcaatga ttccgatcca tttctaaggg
    30661 acaaaggtgc ttttgcaaaa ggcagctttt ttcaaatcat tccttttgtt tcggaatttg
    30721 gagaatatgc gactaaaatc gagcatccat tattggacat ctatacatct aaattagagc
    30781 aaaattattt cggtaaacga ttgaacatgg acttcaacaa acagcttgac agcttttcat
    30841 tagaaaatga aatcgcagta ctcgattata atatcaaatt aaagaactgc ttcttcagct
    30901 aatagtttcg agaaagtcca aaaaggaggt atacattgag atactacttt gtggatgaag
    30961 aaaagaatgt aatagaaaca gatgaaaaaa aatataattg gctcgtactt gatagtgcaa
    31021 agcccgaaga gattgagcaa gcaatcgaag aattcgagct tcctgatgat attttcgtag
    31081 gaaccgaata tccggaagaa gtttctcgga tggagcgcct ttatgagacg aaactagagc
    31141 atccattttc tttggtagtg attaatctag atcagtcaga taatcgtatc gaaaaaaatt
    31201 aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg atagaaaaac
    31261 cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt ttgaaaagat
    31321 catcacgtat acattattag atatttacac ccattacgtg aaagaactaa gagagatgaa
    31381 aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg aagaactgta
    31441 taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa gagatcaacg
    31501 ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag acgatgaaca
    31561 gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg agatctatcg
    31621 agatttatta gagagtattg gtgatctatt caacgggatg atggacaata atctaaatca
    31681 tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt tgttcgcagg
    31741 gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag gctttatact
    31801 cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat accggaaaga
    31861 ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt gttttggcaa
    31921 tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat cgacccgcga
    31981 aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc tttacagcag
    32041 aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa tcaactagaa
    32101 aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc agcgattgca
    32161 tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga aaaagatgca
    32221 gaggaagtag caaaatacgt gaaagttgca ggacgtaaga ttgtcttact accttttgat
    32281 cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga acttggcggg
    32341 ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat tgctgagtta
    32401 ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt tgctttagtg
    32461 aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac ttctgttcaa
    32521 gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc aatcgctaac
    32581 tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa tggtgtagca
    32641 cctggtccaa tctggacacc attacaacta gacgaaggac aaccttctga ttcgatccct
    32701 gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt agcaccagtt
    32761 tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta cggagtaact
    32821 ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag gaggtgcgaa
    32881 aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc tatctgtgct
    32941 tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag agaagtttcg
    33001 attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg ccgttgcatt
    33061 tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa caatcggaaa
    33121 ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa ttgaaggaat
    33181 ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa atgtcaaagc
    33241 aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt cagcactcat
    33301 tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca tattgggaac
    33361 aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag ctaaacgaag
    33421 caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta ttttcgacat
    33481 acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta tggatcaccg
    33541 tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc gctatcggtt
    33601 tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag gaaacatcca
    33661 tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca gtgtctgcac
    33721 atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag aacaaaacag
    33781 caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga gtagaagaga
    33841 agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa tctaaagttc
    33901 cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa catccttctg
    33961 accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa ggaaaacttg
    34021 gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat tcaatgcgcg
    34081 gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca attgaacaag
    34141 tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga aaacttgtga
    34201 atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa gtagcagttg
    34261 atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa gaaatgcaag
    34321 aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa gtcaacgcta
    34381 acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg gtgcaagaca
    34441 aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca gataaagtaa
    34501 aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca cgggttcagt
    34561 aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc acttatcgtc
    34621 ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc gatggggatt
    34681 attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt attaggttct
    34741 tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc catcatttta
    34801 gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa agaggtcgtg
    34861 aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    34921 tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta tacggaataa
    34981 gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt cccaattaga
    35041 tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc tttaataaaa
    35101 caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata atacgaccac
    35161 tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta aaaagcaatg
    35221 gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct tttttactct
    35281 tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata gaagtaaaca
    35341 aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga cgagcaggaa
    35401 aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa caataaatgg
    35461 catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga tatttgtttg
    35521 tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa ataatcatta
    35581 ttatcaaaat aatg
//
LOCUS       DAFFQY010000023        34590 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFFQY010000023 DAFFQY010000000
VERSION     DAFFQY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.023"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1152
                     /locus_tag="LRB02_001019"
     CDS             <1..1152
                     /locus_tag="LRB02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBL4659240.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LRB02_001020"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LRB02_001020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL4659241.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LRB02_001021"
     CDS             2728..3003
                     /locus_tag="LRB02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL4659242.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LRB02_001022"
     CDS             3009..3776
                     /locus_tag="LRB02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL4659243.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LRB02_001023"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LRB02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL4659244.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LRB02_001024"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LRB02_001024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL4659245.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LRB02_001025"
     CDS             7920..8222
                     /locus_tag="LRB02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL4659246.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LRB02_001026"
     CDS             8225..9388
                     /locus_tag="LRB02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4659247.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LRB02_001027"
     CDS             9414..12842
                     /locus_tag="LRB02_001027"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL4659248.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LRB02_001028"
     CDS             12863..13987
                     /locus_tag="LRB02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659249.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LRB02_001029"
     CDS             13977..14414
                     /locus_tag="LRB02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4659250.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LRB02_001030"
     CDS             14398..14736
                     /locus_tag="LRB02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL4659251.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LRB02_001031"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LRB02_001031"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL4659252.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LRB02_001032"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LRB02_001032"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL4659253.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LRB02_001033"
     CDS             15893..16936
                     /locus_tag="LRB02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL4659254.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="LRB02_001034"
     CDS             17095..17823
                     /locus_tag="LRB02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL4659255.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="LRB02_001035"
     CDS             17939..18184
                     /locus_tag="LRB02_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL4659256.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="LRB02_001036"
     CDS             18181..18399
                     /locus_tag="LRB02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL4659257.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="LRB02_001037"
     CDS             18535..19074
                     /locus_tag="LRB02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL4659258.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="LRB02_001038"
     CDS             19067..21340
                     /locus_tag="LRB02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL4659259.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="LRB02_001039"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="LRB02_001039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL4659260.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="LRB02_001040"
     CDS             complement(22513..22767)
                     /locus_tag="LRB02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL4659261.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="LRB02_001041"
     CDS             complement(23167..23436)
                     /locus_tag="LRB02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659262.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="LRB02_001042"
     CDS             complement(23906..24361)
                     /locus_tag="LRB02_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL4659263.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="LRB02_001043"
     CDS             complement(24499..26427)
                     /locus_tag="LRB02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL4659264.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="LRB02_001044"
     CDS             complement(26624..27457)
                     /locus_tag="LRB02_001044"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL4659265.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="LRB02_001045"
     CDS             complement(27649..28353)
                     /locus_tag="LRB02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4659266.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="LRB02_001046"
     CDS             complement(28376..29002)
                     /locus_tag="LRB02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4659267.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="LRB02_001047"
     CDS             29328..31703
                     /locus_tag="LRB02_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL4659268.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="LRB02_001048"
     CDS             complement(31727..32218)
                     /locus_tag="LRB02_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL4659269.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="LRB02_001049"
     CDS             32405..33325
                     /locus_tag="LRB02_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4659270.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="LRB02_001050"
     CDS             33479..33823
                     /locus_tag="LRB02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL4659271.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34018..34512)
                     /locus_tag="LRB02_001051"
     CDS             complement(34018..34512)
                     /locus_tag="LRB02_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659272.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKILAVLKHNKKMGLRFIVKMRKNTI
                     RKSN"
BASE COUNT        11426 a         6057 c         7136 g         9971 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcggttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt atttacagac aaaagtgcct catcaatgcc
    34141 gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc cttttttacg
    34201 ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg tttcgttcag
    34261 tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta aaataaagac
    34321 ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt cgcatttttt
    34381 tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa atccactctc
    34441 atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt cccatagttt
    34501 gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt ctactatagc
    34561 actattgttg taaatagcca tgcctatttt
//
LOCUS       DAFFQY010000024        32760 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFFQY010000024 DAFFQY010000000
VERSION     DAFFQY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.024"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(290..1087)
                     /locus_tag="LRB02_001052"
     CDS             complement(290..1087)
                     /locus_tag="LRB02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL4659273.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="LRB02_001053"
     CDS             complement(1084..2268)
                     /locus_tag="LRB02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL4659274.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="LRB02_001054"
     CDS             complement(2293..3540)
                     /locus_tag="LRB02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL4659275.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="LRB02_001055"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="LRB02_001055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL4659276.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFSSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="LRB02_001056"
     CDS             5520..5834
                     /locus_tag="LRB02_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL4659277.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="LRB02_001057"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="LRB02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL4659278.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="LRB02_001058"
     CDS             6519..7163
                     /locus_tag="LRB02_001058"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL4659279.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="LRB02_001059"
     CDS             7178..7507
                     /locus_tag="LRB02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL4659280.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="LRB02_001060"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="LRB02_001060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL4659281.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9318
                     /locus_tag="LRB02_001061"
                     /pseudo
     CDS             8495..9318
                     /locus_tag="LRB02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
     gene            9311..9658
                     /locus_tag="LRB02_001062"
     CDS             9311..9658
                     /locus_tag="LRB02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL4659282.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9727..10599
                     /gene="rsmI"
                     /locus_tag="LRB02_001063"
     CDS             9727..10599
                     /gene="rsmI"
                     /locus_tag="LRB02_001063"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL4659283.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10708..11829
                     /gene="dinB"
                     /locus_tag="LRB02_001064"
     CDS             10708..11829
                     /gene="dinB"
                     /locus_tag="LRB02_001064"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL4659284.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11883..12485)
                     /gene="nrdG"
                     /locus_tag="LRB02_001065"
     CDS             complement(11883..12485)
                     /gene="nrdG"
                     /locus_tag="LRB02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL4659285.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12800..14989)
                     /gene="nrdD"
                     /locus_tag="LRB02_001066"
     CDS             complement(12800..14989)
                     /gene="nrdD"
                     /locus_tag="LRB02_001066"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL4659286.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15288..16082
                     /locus_tag="LRB02_001067"
     CDS             15288..16082
                     /locus_tag="LRB02_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659287.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16072..18114
                     /locus_tag="LRB02_001068"
     CDS             16072..18114
                     /locus_tag="LRB02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659288.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18395..18544
                     /locus_tag="LRB02_001069"
     CDS             18395..18544
                     /locus_tag="LRB02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL4659289.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18555..20069
                     /gene="dltA"
                     /locus_tag="LRB02_001070"
     CDS             18555..20069
                     /gene="dltA"
                     /locus_tag="LRB02_001070"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL4659290.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20066..21271
                     /gene="dltB"
                     /locus_tag="LRB02_001071"
     CDS             20066..21271
                     /gene="dltB"
                     /locus_tag="LRB02_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL4659291.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21316..21549
                     /gene="dltC"
                     /locus_tag="LRB02_001072"
     CDS             21316..21549
                     /gene="dltC"
                     /locus_tag="LRB02_001072"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL4659292.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21552..22820
                     /gene="dltD"
                     /locus_tag="LRB02_001073"
     CDS             21552..22820
                     /gene="dltD"
                     /locus_tag="LRB02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL4659293.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22961..24043
                     /locus_tag="LRB02_001074"
     CDS             22961..24043
                     /locus_tag="LRB02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL4659294.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24043..24846
                     /locus_tag="LRB02_001075"
     CDS             24043..24846
                     /locus_tag="LRB02_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL4659295.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24916..25656
                     /locus_tag="LRB02_001076"
     CDS             24916..25656
                     /locus_tag="LRB02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4659296.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25667..26671
                     /locus_tag="LRB02_001077"
     CDS             25667..26671
                     /locus_tag="LRB02_001077"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL4659297.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26716..28560)
                     /locus_tag="LRB02_001078"
     CDS             complement(26716..28560)
                     /locus_tag="LRB02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659298.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28772..29289
                     /locus_tag="LRB02_001079"
                     /pseudo
     CDS             28772..29289
                     /locus_tag="LRB02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            29417..30100
                     /locus_tag="LRB02_001080"
     CDS             29417..30100
                     /locus_tag="LRB02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL4659299.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30409..30482
                     /locus_tag="LRB02_001081"
     tRNA            30409..30482
                     /locus_tag="LRB02_001081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30443..30445,aa:Arg,seq:tct)
     gene            30641..31000
                     /locus_tag="LRB02_001082"
     CDS             30641..31000
                     /locus_tag="LRB02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL4659300.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31123..32754
                     /locus_tag="LRB02_001083"
     CDS             31123..32754
                     /locus_tag="LRB02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL4659301.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        10345 a         5721 c         6747 g         9947 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttagc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca
     8761 aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt
     8821 gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg
     8881 gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc
     8941 gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct
     9001 tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg
     9061 aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc
     9121 gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct
     9181 gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg
     9241 actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta
     9301 ggtgagatga atggataaga gatcattata cgacggattg aatcaattag aatcagagtt
     9361 gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa
     9421 aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca
     9481 agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct
     9541 tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga
     9601 caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc
     9661 aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg
     9721 tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt
     9781 gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa
     9841 gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt
     9901 gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa
     9961 ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct
    10021 tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg
    10081 atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct
    10141 caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg
    10201 aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta tcggatcact
    10261 gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa
    10321 ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc
    10381 gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg
    10441 ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc
    10501 gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa
    10561 aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa
    10621 tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg
    10681 ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat
    10741 acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga
    10801 gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt
    10861 gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca
    10921 atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat
    10981 cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt
    11041 gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc
    11101 aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta
    11161 acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa
    11221 aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg
    11281 ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg
    11341 atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga
    11401 aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt
    11461 acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa
    11521 gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga
    11581 gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg
    11641 accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca
    11701 agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact
    11761 ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa
    11821 gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt
    11881 ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt
    11941 tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg
    12001 ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca
    12061 aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca
    12121 aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt
    12181 cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat
    12241 ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat
    12301 ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag
    12361 cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg
    12421 tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt
    12481 ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta
    12541 ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt
    12601 attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact
    12661 tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc
    12721 ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca
    12781 ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc
    12841 ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta
    12901 catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg
    12961 gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa
    13021 gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt
    13081 ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt ggataatcct
    13141 tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt
    13201 ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac
    13261 ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat
    13321 attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag
    13381 cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca
    13441 atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat
    13501 cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg
    13561 gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc
    13621 ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg
    13681 gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt
    13741 cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta
    13801 attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca
    13861 atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca
    13921 aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc
    13981 gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc
    14041 ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca
    14101 aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc
    14161 gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg
    14221 caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg
    14281 ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt
    14341 cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc
    14401 agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt
    14461 tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat tttccaacga
    14521 tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt
    14581 tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt
    14641 tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt
    14701 cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt
    14761 cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat
    14821 aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga
    14881 tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg
    14941 tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact
    15001 cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg
    15061 gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat
    15121 tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt
    15181 ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac
    15241 ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa
    15301 gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca
    15361 gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt
    15421 ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca
    15481 cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga
    15541 aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca
    15601 atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga
    15661 tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag
    15721 aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg
    15781 ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg
    15841 actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag
    15901 caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat
    15961 ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct
    16021 ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat
    16081 aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt
    16141 ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta
    16201 gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc
    16261 attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg
    16321 caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata
    16381 tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt
    16441 attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact
    16501 agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc
    16561 cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt
    16621 tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta
    16681 ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg
    16741 gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt cttttttcct
    16801 tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg
    16861 ttatttttga tcatattgag taaatggaag atttattact atggtatcaa ttttttgata
    16921 atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc
    16981 attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc
    17041 taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa
    17101 atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat
    17161 aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat
    17221 ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt
    17281 tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat
    17341 atttcattat atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa
    17401 atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta
    17461 gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta
    17521 tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg
    17581 ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc
    17641 acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt
    17701 ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat
    17761 atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca
    17821 gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg
    17881 gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt
    17941 ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat
    18001 ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat
    18061 cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg
    18121 gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg
    18181 ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa
    18241 ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact
    18301 gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta
    18361 cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa
    18421 attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt
    18481 cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt
    18541 ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa
    18601 atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac
    18661 aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg
    18721 tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg
    18781 gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc
    18841 gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg
    18901 ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc
    18961 ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg
    19021 gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga
    19081 tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg
    19141 atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa
    19201 ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga
    19261 atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg
    19321 gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa
    19381 ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa
    19441 cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg
    19501 accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta
    19561 aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag
    19621 gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa
    19681 gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac
    19741 gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt
    19801 atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa
    19861 aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc
    19921 agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc
    19981 agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa
    20041 aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc
    20101 gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg
    20161 ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt
    20221 tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat
    20281 acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc
    20341 cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca
    20401 tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca
    20461 aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca
    20521 gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga
    20581 aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt
    20641 ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt
    20701 actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt
    20761 aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt
    20821 tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt
    20881 tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt
    20941 ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag
    21001 caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca
    21061 tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac
    21121 agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac
    21181 gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc
    21241 aggattttta gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca
    21301 aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca
    21361 gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta
    21421 gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct
    21481 gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg
    21541 aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg
    21601 gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga
    21661 ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt
    21721 atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca
    21781 gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc
    21841 tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg
    21901 ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg
    21961 taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt
    22021 caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa
    22081 aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac
    22141 ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg
    22201 aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac
    22261 gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac
    22321 gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag
    22381 cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat
    22441 tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca
    22501 tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac
    22561 aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg
    22621 attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga
    22681 aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg
    22741 ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag
    22801 ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt
    22861 tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat
    22921 ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca
    22981 gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga
    23041 agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag
    23101 tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga
    23161 cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct
    23221 tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg
    23281 gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag
    23341 aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga
    23401 agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg
    23461 aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt
    23521 ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg
    23581 aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa
    23641 tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc
    23701 aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat
    23761 gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc
    23821 attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag
    23881 agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc
    23941 agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg
    24001 gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct
    24061 atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact
    24121 tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc
    24181 agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt cgataattta
    24241 cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc
    24301 agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt
    24361 ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc
    24421 atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta
    24481 cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg
    24541 atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca
    24601 tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt
    24661 ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat
    24721 ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg
    24781 tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt
    24841 acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt
    24901 gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa
    24961 aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca
    25021 ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg
    25081 atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg
    25141 caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat
    25201 tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa
    25261 gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta
    25321 gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt
    25381 gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca
    25441 gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct
    25501 ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt
    25561 ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata
    25621 gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc
    25681 aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat
    25741 gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag
    25801 aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt
    25861 tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag
    25921 ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat
    25981 catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct
    26041 agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt
    26101 tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt
    26161 aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat
    26221 caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat
    26281 cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga
    26341 tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa
    26401 ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat
    26461 tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt
    26521 cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa
    26581 agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga
    26641 accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat
    26701 ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg
    26761 atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt
    26821 catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa
    26881 taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc
    26941 gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa
    27001 ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc
    27061 ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta
    27121 acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt
    27181 ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat
    27241 taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg
    27301 gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa
    27361 atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat
    27421 aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat
    27481 acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg
    27541 tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt
    27601 gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc
    27661 aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa
    27721 aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc
    27781 tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat
    27841 accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa
    27901 gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa
    27961 ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga
    28021 ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc
    28081 atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg
    28141 taaatctatc acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg
    28201 gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa
    28261 atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc
    28321 cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt
    28381 atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat
    28441 aaaataaaag aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct
    28501 ccactttata cgtaaataaa aagattcttt tttattatat atgttattaa aaaaagacat
    28561 aaaggtattc ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa
    28621 ttatttataa taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt
    28681 aaagggaaac atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag
    28741 aaggattgaa taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat
    28801 aagttctata acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg
    28861 aatttttggt ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca
    28921 aggaaatctg atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat
    28981 tacatattat gaaaattcag gaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac
    29041 aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta
    29101 aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt
    29161 tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca
    29221 ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa
    29281 tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca
    29341 tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt
    29401 gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact
    29461 attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga
    29521 agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt
    29581 tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc
    29641 atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt
    29701 cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt
    29761 gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt
    29821 tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt
    29881 gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct
    29941 cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct
    30001 tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt
    30061 gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg
    30121 tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt
    30181 aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc
    30241 tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt
    30301 gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg
    30361 acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct
    30421 cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga
    30481 cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat
    30541 attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag
    30601 gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat
    30661 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
    30721 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
    30781 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
    30841 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
    30901 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
    30961 tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt
    31021 tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat
    31081 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
    31141 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
    31201 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
    31261 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
    31321 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
    31381 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
    31441 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
    31501 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
    31561 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
    31621 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
    31681 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
    31741 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
    31801 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
    31861 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
    31921 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
    31981 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
    32041 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
    32101 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
    32161 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
    32221 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
    32281 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
    32341 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
    32401 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
    32461 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
    32521 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
    32581 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
    32641 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
    32701 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
//
LOCUS       DAFFQY010000025        31749 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFFQY010000025 DAFFQY010000000
VERSION     DAFFQY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.025"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            363..731
                     /locus_tag="LRB02_001084"
     CDS             363..731
                     /locus_tag="LRB02_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL4659302.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="LRB02_001085"
     CDS             911..1852
                     /locus_tag="LRB02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL4659303.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="LRB02_001086"
     CDS             1921..2652
                     /locus_tag="LRB02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL4659304.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="LRB02_001087"
     CDS             2796..4976
                     /locus_tag="LRB02_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL4659305.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="LRB02_001088"
     CDS             4996..5478
                     /locus_tag="LRB02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL4659306.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="LRB02_001089"
     CDS             5494..6255
                     /locus_tag="LRB02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL4659307.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="LRB02_001090"
     CDS             6386..7720
                     /locus_tag="LRB02_001090"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL4659308.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="LRB02_001091"
     CDS             8027..8512
                     /locus_tag="LRB02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL4659309.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="LRB02_001092"
     CDS             8505..9188
                     /locus_tag="LRB02_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL4659310.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="LRB02_001093"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="LRB02_001093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL4659311.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="LRB02_001094"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="LRB02_001094"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL4659312.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="LRB02_001095"
     CDS             12804..13433
                     /locus_tag="LRB02_001095"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL4659313.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="LRB02_001096"
     CDS             13485..13817
                     /locus_tag="LRB02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL4659314.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="LRB02_001097"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="LRB02_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL4659315.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="LRB02_001098"
     CDS             14643..16016
                     /locus_tag="LRB02_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL4659316.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="LRB02_001099"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="LRB02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL4659317.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="LRB02_001100"
     CDS             17071..17511
                     /locus_tag="LRB02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL4659318.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="LRB02_001101"
     CDS             17655..18356
                     /locus_tag="LRB02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL4659319.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="LRB02_001102"
     CDS             18353..19474
                     /locus_tag="LRB02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL4659320.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="LRB02_001103"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="LRB02_001103"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL4659321.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="LRB02_001104"
     CDS             20853..21761
                     /locus_tag="LRB02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL4659322.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="LRB02_001105"
     CDS             21796..22068
                     /locus_tag="LRB02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL4659323.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="LRB02_001106"
     CDS             22079..23125
                     /locus_tag="LRB02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL4659324.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="LRB02_001107"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="LRB02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL4659325.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="LRB02_001108"
     CDS             complement(26136..26822)
                     /locus_tag="LRB02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659326.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="LRB02_001109"
     CDS             complement(26800..27294)
                     /locus_tag="LRB02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL4659327.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="LRB02_001110"
     CDS             complement(27314..28534)
                     /locus_tag="LRB02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659328.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="LRB02_001111"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="LRB02_001111"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL4659329.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31736)
                     /locus_tag="LRB02_001112"
                     /pseudo
     CDS             complement(30610..31736)
                     /locus_tag="LRB02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT        10569 a         5670 c         6793 g         8717 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atgaaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcttt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaaccatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaacctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt tcttgagcca
    31681 tcatcattga tatcttcgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
    31741 gttacactc
//
LOCUS       DAFFQY010000026        31660 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFFQY010000026 DAFFQY010000000
VERSION     DAFFQY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.026"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..343
                     /locus_tag="LRB02_001113"
     CDS             <1..343
                     /locus_tag="LRB02_001113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659330.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="LRB02_001114"
     CDS             424..1044
                     /locus_tag="LRB02_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659331.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="LRB02_001115"
     CDS             complement(1374..2600)
                     /locus_tag="LRB02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4659332.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="LRB02_001116"
     CDS             complement(2672..2830)
                     /locus_tag="LRB02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659333.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..4258
                     /locus_tag="LRB02_001117"
     CDS             3518..4258
                     /locus_tag="LRB02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4659334.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4350..5537)
                     /locus_tag="LRB02_001118"
     CDS             complement(4350..5537)
                     /locus_tag="LRB02_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4659335.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5859..6701
                     /locus_tag="LRB02_001119"
     CDS             5859..6701
                     /locus_tag="LRB02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4659336.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6701..7648
                     /locus_tag="LRB02_001120"
     CDS             6701..7648
                     /locus_tag="LRB02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL4659337.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7663..7896
                     /locus_tag="LRB02_001121"
     CDS             7663..7896
                     /locus_tag="LRB02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL4659338.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8227..8811
                     /locus_tag="LRB02_001122"
     CDS             8227..8811
                     /locus_tag="LRB02_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4659339.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8942..9610
                     /locus_tag="LRB02_001123"
     CDS             8942..9610
                     /locus_tag="LRB02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL4659340.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9621..11036
                     /locus_tag="LRB02_001124"
     CDS             9621..11036
                     /locus_tag="LRB02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4659341.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            11054..11623
                     /locus_tag="LRB02_001125"
     CDS             11054..11623
                     /locus_tag="LRB02_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL4659342.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11733..13487
                     /locus_tag="LRB02_001126"
     CDS             11733..13487
                     /locus_tag="LRB02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659343.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFVRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13447..15213
                     /locus_tag="LRB02_001127"
     CDS             13447..15213
                     /locus_tag="LRB02_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659344.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            15736..16005
                     /gene="rpsN"
                     /locus_tag="LRB02_001128"
     CDS             15736..16005
                     /gene="rpsN"
                     /locus_tag="LRB02_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL4659345.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16020..16199
                     /gene="rpmF"
                     /locus_tag="LRB02_001129"
     CDS             16020..16199
                     /gene="rpmF"
                     /locus_tag="LRB02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL4659346.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16231..16608
                     /locus_tag="LRB02_001130"
     CDS             16231..16608
                     /locus_tag="LRB02_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659347.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITEMGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16629..16778
                     /gene="rpmG"
                     /locus_tag="LRB02_001131"
     CDS             16629..16778
                     /gene="rpmG"
                     /locus_tag="LRB02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4659348.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16802..17785
                     /locus_tag="LRB02_001132"
     CDS             16802..17785
                     /locus_tag="LRB02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL4659349.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17815..17937
                     /locus_tag="LRB02_001133"
     CDS             17815..17937
                     /locus_tag="LRB02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL4659350.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17955..19478
                     /locus_tag="LRB02_001134"
     CDS             17955..19478
                     /locus_tag="LRB02_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL4659351.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19502..19741
                     /locus_tag="LRB02_001135"
     CDS             19502..19741
                     /locus_tag="LRB02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659352.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19934..20395)
                     /locus_tag="LRB02_001136"
     CDS             complement(19934..20395)
                     /locus_tag="LRB02_001136"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4659353.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20590..20808)
                     /locus_tag="LRB02_001137"
     CDS             complement(20590..20808)
                     /locus_tag="LRB02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659354.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20926..22542
                     /locus_tag="LRB02_001138"
     CDS             20926..22542
                     /locus_tag="LRB02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL4659355.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22783..24123)
                     /locus_tag="LRB02_001139"
                     /pseudo
     CDS             complement(22783..24123)
                     /locus_tag="LRB02_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24399..24554)
                     /locus_tag="LRB02_001140"
     CDS             complement(24399..24554)
                     /locus_tag="LRB02_001140"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL4659356.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24746..24940)
                     /locus_tag="LRB02_001141"
     CDS             complement(24746..24940)
                     /locus_tag="LRB02_001141"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4659357.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(24850..25182)
                     /locus_tag="LRB02_001142"
     CDS             complement(24850..25182)
                     /locus_tag="LRB02_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4659358.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25696..25887)
                     /locus_tag="LRB02_001143"
     CDS             complement(25696..25887)
                     /locus_tag="LRB02_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659359.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25981..26367)
                     /locus_tag="LRB02_001144"
     CDS             complement(25981..26367)
                     /locus_tag="LRB02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4659360.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            27061..28266
                     /locus_tag="LRB02_001145"
     CDS             27061..28266
                     /locus_tag="LRB02_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL4659361.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            28283..28717
                     /locus_tag="LRB02_001146"
     CDS             28283..28717
                     /locus_tag="LRB02_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659362.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            29334..>31660
                     /gene="esp"
                     /locus_tag="LRB02_001147"
     CDS             29334..>31660
                     /gene="esp"
                     /locus_tag="LRB02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL4659363.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT        10866 a         4613 c         5824 g        10357 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta ttggagagac taactatata gagtcaagta aaaattagcc
     4261 aaatcaaaaa atattttata acagataatt ccgaaaaaag ggtacaacaa ataagcttct
     4321 taccattagt ttttttgaat gttcaatagc taatcagaaa caattatata aggctcgttt
     4381 ttagtcaaaa tagcatgaat cacgtaacaa agcttacgag aaaccgcacc taaggcagtt
     4441 agatgatgtt ttccttcgga aacttttttc tggtaaaacg cactaaaaac aggatcacat
     4501 tgagaagcta ctaaagccgc cgaaaacaag gcttttcgca aatatggaga ccctcgttta
     4561 ctcataacat tatgcgttgc ctcatattgc cctgattgtg tcacggaagc gtctatccca
     4621 gcgtaagcaa cgagtttttt cgggttagaa aatttttgaa tatctccgat ctcaccaaga
     4681 atagtcgcac cgtttatagg accaattcca ggaattgtga gaataacaga atttaaagtg
     4741 tccattatgt gcttgatttc tctttccgtt tcctttattt ggctttcaat gaattttaat
     4801 tgttcaatca ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt tactccaaag
     4861 gagttagaag ctgcttgctt caattggtcc gctttttctg ccttgattcg gttcctgctt
     4921 aactcagcta agtattgggc caaagtttct gaggaaatgt tttctaagtc gatcggggca
     4981 ctaaacgcca gcaataactc tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc
     5041 gtcgcgtatt ctggaaagac ttgatccaat acggctataa tttttctttt gaagtcgctc
     5101 gctgtaccta ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa aacattttca
     5161 tcggataaga ccgtttcgac aaatgagcca tagcgcatta agtcagcaat caaaacagaa
     5221 tcaataatat cgttttttcg tttccgtatt tctgtacctt tacgccatcc gtctgtttga
     5281 aggggattca caacgtgaat caaataatct gattcgtgaa ggtatgaaaa gatggacagc
     5341 caatagtgcc ccgtggcttc catccctata acaaagtcac tgggtgctcc ggaatagtcg
     5401 accattcgct caattaatga ctctgctcct tcagtggtgt taggaaatga aaaacctttt
     5461 agtaatacct ttccttcctc gttcatgata gaagcaacgt gagtgcgttt accgatatca
     5521 atgcctaaaa agtacatgta tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca
     5581 ctcaactgaa aagcgtcact accttgtacg agatacggag taaagtaagg actttcaaca
     5641 tctaactcat acgcaatcca cttatcagag agaggagcca gtctctcgag tgcgggttca
     5701 atggcccaag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     5761 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     5821 ggttaaaacc ctaactctaa tatacaaggg ggaataatat gcttcaaaat tttattaatg
     5881 gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt atagggattg
     5941 tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg ggtgatgcta
     6001 tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt aattttttta
     6061 ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata aaaagcaata
     6121 gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta gcattaggag
     6181 ttatccttat aggagttgca aatagctcaa cggatttatt tcatatctta tttggaaata
     6241 ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttctgtt ttagtgttgg
     6301 ttacagttgt tctatttttt aaagaattat taatcacttc ttttgatcca ttaatggcaa
     6361 aagcaatggg gatgaatgtc agtttttatc attatctatt aatgattcta ttaactttag
     6421 tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg ttaattacac
     6481 cagctgctac agcatattta tatgcaaaca gcttaaaaac tatgatattt atttcggcga
     6541 cagtcggagc gtgctcatca ttgctaggtt tgttcattgg ttatagtttt aacatagcag
     6601 caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt ctaatctcac
     6661 caaaacaaaa atttttaaga aagaaggaga gaaagttatg atgagaaaat ggaaagcagt
     6721 actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt caacaaatag
     6781 taaagacaaa gatacagtgg cttcgaacga aaaattaaag gtagtagtta ctaattcgat
     6841 tttagcagat attactgaaa atatagcaaa agataaaatt gatttacaca gtatcgtacc
     6901 tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa aaacttcaaa
     6961 agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg cttggtttac
     7021 aaaattagta aaaaatgcga acaaagagga aaacaaagac tattttgcag caagtgatgg
     7081 catagatgtt atttacttag agggtcagag tgagaaaggg aaggaagatc cccatgcttg
     7141 gttaaattta gaaaacggta ttatttacgc taaaaatatt gaaaaacagt tagcggaaaa
     7201 agatcctgat aataaaaaat tctataaaga aaatctagat aagtatattg aaaaattgga
     7261 ttctctagac aaagaagcta aatctaaatt tgcttcaatt ccgaatgata aaaaaatgat
     7321 tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc cttctgctta
     7381 catttgggaa atcaacactg aagaagaagg aacaccagat caaataaaac acttagttga
     7441 aaaattacgc acaacaaaag ttccctcctt attcgtagaa agcagtgtgg acgatagacc
     7501 aatgaaaaca gtatcaaaag ataccaatat tcctatctat tcaacgattt ttactgattc
     7561 aattgcagaa aaaggacaag atggtgatag ttactatgcg atgatgaaat ggaacctgga
     7621 taaaattgct gaaggccttt cgaaataatt tggagggtag gtatggtaca aattaaaacg
     7681 actactatac taatttattt actagcaatt caggaattat caaaaagaaa aaagaaatta
     7741 aaaaataatg atttagcaaa agttctaagt gttagtccag catctgtaag tgagatgctg
     7801 gctaaactta gatatgagga ctatttagat ataggatttc aattaactga taaagggaaa
     7861 aaatttattg acgattacaa aaaaggtttt aagtaaaaga ctttattttt agggaggata
     7921 agaaaatggc tattggagtg attatgatag gacttaccct aatagctgga tataattttt
     7981 ttaaatcttt ctcggattat tttagaaaag gtgaatgatt tagatgagtc tattaattct
     8041 tgtaggattg ctttttgtat cagttatttt gctctttctc attagtttag ctctaaggaa
     8101 tgagttcagt aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga taatatagtt
     8161 aattcatctt attaggagga agtacccata gaaaccagag attatattac aactcaccaa
     8221 aaattaatag aaagtggact aagccatttt gaaaaagaag gatttcagca aactaagata
     8281 aggcaactgt gcttgaccgc aggcgtaact acgggtgctt tttataaaca ttttacttct
     8341 aaggaagcgt tatttgaaga aattattcag ccgcatatta atgcgcttat taatatgtat
     8401 aatgaagagt ttaccaaata cctcttaaac ttagaaaata tagatataga gaaaagttgg
     8461 agagagaact tcaatgaatt agatccattt attaattata tttttgagaa taaacgtata
     8521 ttcgatttgg ttttattcca atcagctgga accaaatatc aagatattgt tgaaaaaatt
     8581 acatcatttg tgacaaaaaa gacagtctgt tgtctagata agttaaagac tatcggaatg
     8641 atcaatcaaa atttagtctt taatgaagaa gaaatccact tctacattta ctcattctac
     8701 gctacattct atgatatatt gaagcatagc tattcaaaag aaaagactat gctattaacc
     8761 agaagattgt atcaattttc tgttcctggg tggattgaat tactgagtta attaaaaaaa
     8821 agcttgcatt gagaattgtt ctcagttatg ctttttgata agatcgcact gttatctgaa
     8881 taacggtgca tttttgtcag cttaagataa cagtgcgatc ttattttttg gaggtgaaag
     8941 agtgtcaaaa atcaatccgt taaccttgat tactttaaat attttttttc cggtcattat
     9001 ttttttagga aagggaaata tatttcaagt ggggtgtttc attattgcta cagttttagt
     9061 attgatgttt cgtaaaataa agacatgtat ttatttcatc ttgatttatt cagttctgtt
     9121 tttagtaatg aaaattattg atgctatcaa gatagagttt tttactatat tttttgggac
     9181 atccttatat attgtatttc gaatgttgcc ggtcgtaatg atctcatggt tgttggttag
     9241 tgtctatact agcaatgaat tattatcctc tttagaaaaa atacatttac cgaaaaaaat
     9301 aatgttaagt attacagtag tgatcagatt ctttccgact tatcgttctg aaataagaat
     9361 gattaaagaa tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc ctctgcaata
     9421 tttggaattt ctgttagttc ccgttcttat acgtgcaact atgattgctg aagaaatgac
     9481 cgctaatgca ataacaaagg ggatagaatc tcctgtgaaa agaagttctt actataaaac
     9541 aaacatgagc ttattagact atatcatcct gttttccgtt actatttttt tcttaagtat
     9601 tttgatttga ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca tataaggatg
     9661 ttaaggaaca agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt cttacattga
     9721 ttactggttc ttctgggagt gggaaatcca cactattaaa gttaatgaat ggacttattc
     9781 caagacttta tgacggaaaa ttggttgggg aaattttatt tgaaaataac tcgctacaat
     9841 ccttaacaca agaaagtata tcaaaaaata ttggctacgt aagtcaaaat cctagaggac
     9901 aattctttac aacaaataca acgagtgagt tagtattttc gatggaaaac tttggattta
     9961 caaaagagca tatggataaa agaatcaatg aattagctga attattagat ctttcaaata
    10021 tattagataa aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt tctattggat
    10081 gtagtttatc gctaaactct aaattaatta tattggatga accgtcttca aatttggact
    10141 tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa ggatttacta
    10201 ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa gttctatttg
    10261 ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa aacaataacg
    10321 cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc gaaaaggtaa
    10381 gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa gacataaatt
    10441 ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg gggaaaacaa
    10501 ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata atcgaaaata
    10561 aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa tttttcacag
    10621 aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat cagcaagaaa
    10681 aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt gatttatccg
    10741 gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact aatttatttc
    10801 tatttgatga acctacaagt gggttggatt atatcaatat ggaaaggatt gctgacctta
    10861 ttaaagactt aaaagaaaag ggtgcagtag ttatagccac tcatgatccg gaatttcttt
    10921 ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa gatataaaac
    10981 ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt
    11041 gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta cagttatttc
    11101 cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt ttggtcacag
    11161 catatcccca gggatttttg gattattatc tggaatcata tttgtttata ttagctataa
    11221 ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat tttttacgtt
    11281 aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga tcttagctga
    11341 tattattcta tctgtatttg gatatgatcg agcaattcct caagttgtgt cttgggttct
    11401 aatgcaacta ggcagtgctg ccggacagtg gattcccata tggttcttta cagatcgttt
    11461 tcgtcaagac tggatagata gaggacaatc tgctgcaaca atggatgcaa tgatccacta
    11521 cgcggtgggt atatggggaa ttatttcagt gttagttgta gcaagtttat ctatgattgg
    11581 tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag tttaccttct
    11641 atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt gtaaacgaat
    11701 aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa ttatttagtt
    11761 atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat ttttctgtcg
    11821 tattaacagt ctatggatat tacagtattt acaaatttct tcagtctcta attattgtag
    11881 gagatgaata tttagctaag agttatgcgg ttcaaactgt tgcttggtta actttaggag
    11941 ctttatttta tattttttct ggcttatttt ctcatatcct tgggtttcgc ttagagacta
    12001 atttgagaaa aaagggaatt agcggtttaa cagaagcaag ttttcgtttt tttgatttaa
    12061 attcttcggg atatattcga aagacaattg atgacaacgc ggctaaaaca catatggcaa
    12121 ttgctcactt gattccagat agtgctcaag ccatagtaac acctgtttta tcagttgcct
    12181 taggcttttt cattaacttt aaaattggat tggttctaat cgccatgtta gtaattggtg
    12241 tattcttgtt aaagaaaatg acagagaata caaattttat tcaaaaatat caagaatcgt
    12301 tagatgtgtt aagctctgaa acggtagaat atatccgcgg aattcaagtt ataaagatat
    12361 ttggtgctaa agtaacatca tttaaagcct tgaataaagc aattaacgat tatgctaagt
    12421 ttgcatataa gtattctttg agtgggaaaa caccttttgt tctttttcaa tggctatttt
    12481 ttggcatagt ttgcattttg atagttccta taaccttttt tataacttct cttaccactc
    12541 ccaagatctt agctgttgac ttgttgatgt tgtttttttt aagtggaatt atttttgttt
    12601 ctttcgtgag aataatgtat gtaagtatgc acttgtttga agcaagatat gcagtcgaaa
    12661 cactagactc cctttattta gaaatgaagc aagaccaact aaaatatggg aacgagacac
    12721 agattaaaaa ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa gaagagtgtg
    12781 ttttagaaaa tttcaactta gttttagaac aaaaaagaat ttatgcatta gtaggaaaat
    12841 caggaagcgg aaagagtacg atagctaaac taatttcagg tttctacaat gtggataagg
    12901 gacagattaa aattggtggt aagcctttaa atcactattc aaaagacaca attattaaca
    12961 caattgcttt tgtctttcaa gatacgaaac tattcaatca gagtatttac gataacgttt
    13021 ctatagcaaa tcctcaagcg ccagcaagta aagtatttga cgcgctaaga ttagccggtt
    13081 gcgaatcgat tttagaaaaa tttcccgaaa gagagcatac cttaattggt agtaaaggag
    13141 tttatctgtc aggaggagaa aaacaacgta tagctattgc cagagcaatt ttaaaagatg
    13201 caaagattgt tattatggat gaagccagtg catctattga tccagataat gagtatgaac
    13261 tacaaaaggc ttttcaatat ttgatgaaag aaaaaacagt tattatgata gctcatcgat
    13321 tatccagcat taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa atagtagaac
    13381 gtggaaatca taatgaatta atggctaaac atcgagtgta taaacaattg gtcactaatt
    13441 atgagaatgc aaatgattgg aggcttgaca atgaaaaatt tttataaaaa aaaatttgct
    13501 ttaactgatc agggcgcaga agctttaact aaagcttcta tttctagttt ttttgtatac
    13561 tgcattaata tggtaccagc attcattata atgatgctga tagacgaatt agttttagaa
    13621 aatgcaaagc cccgttggct ttattttgca gtatcgtttg ttactttgct tttcatgtat
    13681 tggttgttag atagggagta tgaaaactta tataatagta cttataaaga aagtgcgcat
    13741 ttaagagtgc aaattgcaga cgatttgtca aatttaccat tatcctattt ttcaaaacat
    13801 aatttatcag atttatctca aactatcatg tctgacgttg aaggtattga gcatgcgatg
    13861 agtcatgcaa tacctaaagc cggcggtatg gctctgtttt tcccttttat ttcagtgatg
    13921 cttttggttg gtaatgtcaa aatgggatta gctgttattt tgccaacgtt atttagtttt
    13981 gtcttaatct tgttatcaaa gaaatcccaa acgaaagcca atactaaata ttacgatact
    14041 ttgagagaaa actcggaaga atttcaagaa actattgaat tgcagcaaga gattaatagc
    14101 tttaatctat ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga gagtgaaagg
    14161 attcatttaa aggtagaatt aagtactttt tcagtcatgg ccttatcctc tattttctca
    14221 tatgttagtt tagcagtagt cattctagta ggtgttcact tactgttaac gggtgaagtg
    14281 actatactct acgtcgttgg ttacttacta gccgcaataa aaataaagga ttccttcgat
    14341 tctatgaaag aagcagtgct tgaaatattt tatttagctc ctaaaataca gcgaattaga
    14401 gctatgaagg aaaccagtat ccaagaggga tcagatagtc ctctgaaatc ttttgacatt
    14461 gagttgcgag atgtctcttt ctcatatgat aacaataccc caattttaga tcatatctct
    14521 tttacggcta aacaaggaga agtgactgcg ttagtaggtg ctagtggttc cgggaaaact
    14581 agtatcttga aattagtgtc tagactgtat gattatgatg aaggctgtat tttaattgat
    14641 gggtatgata ttaaacgtgt atctccagct tctttgttta gcaaaatcgc tattgttttt
    14701 caagaggtaa ctttgtttaa tacaagtatt ttggaaaata tacgtattgg aaatagtcaa
    14761 gcgtcagatg aggaagtgaa aaaagcagct cgtttagcta actgcgaaga ctttattgaa
    14821 aaactacctg acggttatca tacattggtc ggagaaaacg gaagttcact ttctggagga
    14881 gagcgacaac gcctatccat agcacgggct tttttgaaaa atgcgccaat tttaatttta
    14941 gatgaaatta ctgccagttt agatgcggaa aatgaaaaac gtattcagga aagtttaaat
    15001 cgtttaatac aagataagac agtactaatt atatcgcatc gcttgaagtc tattgaaaag
    15061 gtaaataaaa ttgtagtgat ggatcaagga aaactagttg atcaaggtac tcataatgag
    15121 ttgtatcgaa ggtcagaaat atacaaaaat ctcataaaga aaactaaact atctgagaaa
    15181 ttcgtctatg aaaaagaagc tcaatcacga tgatgaaata ggctttaaat taacctacaa
    15241 tttttgggat atagttataa ttctttttca aaaagaataa ttttattaaa cagcatctaa
    15301 ttttgaatac acgaaagaat cagtatgatt gaattagttc acttaatgtc tattcttata
    15361 atataaggaa taatcaaagt tttaaaaaat tggcgataaa ccagttaaac ataaaagatt
    15421 gaataaaaag ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa
    15481 acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag agcctaaact
    15541 gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt
    15601 tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg
    15661 gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat caaaataaaa
    15721 ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg
    15781 atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct
    15841 ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat
    15901 ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt
    15961 gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca
    16021 tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa
    16081 ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc
    16141 atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt
    16201 ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa
    16261 gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat
    16321 cgttcgatgg tagagagagg aattattact gaaatgggtt taccgaccca acaggcaatt
    16381 gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta
    16441 agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat cgatggttat
    16501 tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta
    16561 acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag
    16621 gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc
    16681 tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa
    16741 aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa
    16801 aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat
    16861 caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga
    16921 gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca
    16981 actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc
    17041 tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac
    17101 aggtatagca gatccccaac caatcattca aacactttta acagcgcctc aactaagagg
    17161 aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga
    17221 aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg tgtctagaaa
    17281 acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc
    17341 tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa
    17401 tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa
    17461 tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc tcttaacttc
    17521 agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga ttttaaccaa
    17581 ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg attttatagt
    17641 tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca
    17701 aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga
    17761 taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt tctaatggag
    17821 aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa aactagaaaa
    17881 agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt agcataatta
    17941 aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat
    18001 tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat
    18061 tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg
    18121 aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag
    18181 ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg
    18241 aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag
    18301 gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag
    18361 aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg
    18421 aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg
    18481 agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta
    18541 gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt ggatatttag
    18601 ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa gaagagccat
    18661 cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt
    18721 actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa gctgggataa
    18781 aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag
    18841 attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact atggttgctg
    18901 gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact
    18961 ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg
    19021 tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaaatca
    19081 ataaagacat gacagctgcc gagtacaaag attattatac tactggctat aagacagata
    19141 ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc
    19201 aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg
    19261 ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta
    19321 gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa
    19381 gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca gcttcattgt
    19441 ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata
    19501 catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta
    19561 tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt
    19621 acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat
    19681 caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta
    19741 attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat
    19801 gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc
    19861 caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag
    19921 ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta
    19981 ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat
    20041 tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg
    20101 ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag
    20161 cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca
    20221 aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc caatgaccaa
    20281 aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta aggcaaatct
    20341 ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac
    20401 ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg
    20461 cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg
    20521 agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc
    20581 acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt
    20641 ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta aaatagtaaa
    20701 cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag
    20761 aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa ttattttcct
    20821 tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact catttttatg
    20881 tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaaatacct
    20941 attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa tcatattctg
    21001 tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga agtaaatgtt
    21061 gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg
    21121 ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact agatcaatta
    21181 gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag caaaccaatt
    21241 ccaattattc aagcaattat attagctaaa aaccctttag acattgattt gacccaacga
    21301 ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga taatttcgaa
    21361 acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga agaacaggat
    21421 attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt aaataatagt
    21481 gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa gttacagtcc
    21541 aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat tctaaataca
    21601 aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga gttagagctt
    21661 ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt tatctatcga
    21721 aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa ttttcctggc
    21781 aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt ttcgacgatc
    21841 tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt agctagttta
    21901 cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga agcctggcat
    21961 ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa
    22021 tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga
    22081 aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc gttaatgaaa
    22141 tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt
    22201 acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat
    22261 gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt cgatttggat
    22321 aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag
    22381 attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga
    22441 actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt
    22501 agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa tagatgagga
    22561 ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt
    22621 aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac
    22681 ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg
    22741 ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc
    22801 tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt tcttaaaata
    22861 ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat agccataggc
    22921 aattcgtttt agtactttga tatggttatt caaacattct atagggccgt tggaataggg
    22981 aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga acactggttg
    23041 gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc
    23101 ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc
    23161 atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc gaaatgacgg
    23221 acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta gtttccagta
    23281 tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg
    23341 gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg cgacgattcg
    23401 ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac
    23461 tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt
    23521 gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa
    23581 gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca ccacttctgg
    23641 taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc gaatgacagt
    23701 ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt cgcttaatag
    23761 ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac taactgaatc
    23821 ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcag
    23881 aatggtcttg tagttactga catcattcaa taagatggat acttttttcc agccctattt
    23941 aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt gataggtcaa
    24001 tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa aggcatcttc
    24061 atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga
    24121 catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga ttttaaggtg
    24181 tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact
    24241 agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt
    24301 gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc
    24361 tctattttac ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac
    24421 gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg
    24481 tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga
    24541 attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc
    24601 atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac
    24661 aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca aacagcatca
    24721 atttggttct gttcaaatta tcgattcaac atttttttac catttctttg attgtttttt
    24781 ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc atttcacgca
    24841 taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt ttttgagtga
    24901 tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg
    24961 tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc
    25021 agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa ttgttggacg
    25081 agagatattg tattcttcag caattttctt ggtagttact tttttctcaa ggatatcctg
    25141 tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc attccttccg
    25201 gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc agggtttgtc
    25261 aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct cttgactatt
    25321 tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg tcgttacgga
    25381 aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta ccatagccca
    25441 gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta ggagtgagac
    25501 tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa ttaaaatcat
    25561 tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt ataattataa
    25621 agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact tatgtcacag
    25681 actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat atattgcaag
    25741 catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt gcttatgaat
    25801 caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag cctttgctaa
    25861 aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac ttataatcta
    25921 tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat tatgcagata
    25981 ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg tcgcaccctg
    26041 tcttgtaatc cccagaacct ttccaatttc tttatcagtt ttatcaaaga taaatttttc
    26101 gataagaaaa atttgttcag gttctttcaa caaaggaagg atttttgcta attttatatc
    26161 ttcaacaagg agagtcacat ttaaaacatt aatcggttgt ttaaatttta tagtatcttt
    26221 agtaaacaaa tatggtggga gttgatcaag ttgttcatgt ttttgaattt cttcttgttt
    26281 tttataataa gtattagcgg tgttcaaaat gaccttttca aagaatcgaa tcaactgggc
    26341 atttacgcgt tcgtcgttat ctgacatatt gcacacctcc tccattaaga tttaaaggga
    26401 aaagaggcaa aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg aataacttct
    26461 ataagctcac atgcattgat aggactgctg atgtttattt tcaaaaatgt ttttttagtt
    26521 gataatggat agttatcttt atacttagaa aagaaaattc atatatcttc ttaaatatca
    26581 aacatgtcct tatggctaag aaaatgtctt gagcttttta cgtataataa atcttttcta
    26641 aaacctcctt ttggataata ttcttattcg tgtatagatt gtatttttta tttcttcccc
    26701 cttcataatt aacaactaga aaagagttcg aatagacacg tatttgaacg aaaattttta
    26761 ttggattccc aaattggaat atttagaaac gaaaaattcc tattatctgt atagtgctta
    26821 gataataacg gatcataata attattgtct ttgaactaga tatatgtaaa tagtattcat
    26881 catgatgaaa aatgatgagt tctcggtgat aaaatgtact ttttacggtg gaatatacat
    26941 gaaagcgata agtggatatt ttataatgaa gtaagttagc ttattttgac agaggaatag
    27001 tattttagat aacgtataaa atactattta atgatgtaga aaggaaagag agaaacaaaa
    27061 gtgttttcag ataaagaagt cttattaaag tctcttcatg tttgtttaaa gttacctatc
    27121 gtggttttta attccgaatt cagtattata aaagagtatc gctcagatag aactacttat
    27181 tttttttatg attataaaaa gttactaact gataatatta aaaaaactaa agatttccat
    27241 ttctttactg gtgattttaa tgagatgttt cttctataca tgtttaaaac cagatattac
    27301 ttatttggtc catttagagc aaataatatt gataaagttt tttttaaact aaaaatgaac
    27361 aatttgaatg ttgcaatggc tgatagagaa cgactctata attcattgca aaatttgact
    27421 ttgtattctt taggagacat tcgagatatt ctgattttag ttcattattt ttttactgga
    27481 aaaatagaag atctattcca tgaaccgtta attgaatata cagaaaatct aagtaaaact
    27541 atcgagcaaa tcaaaataga taatctgtta tcacaaaatt acgatccaga aatatatctg
    27601 tttctttatg aaaataaaat actggaatat gttaaaaatg gagatattcg aaatttagaa
    27661 aacatggttt ttaatctgag taatggaata attccaagtg ttagtggaga tacgattcgg
    27721 tctgaaaaaa attattctat cattgttttt gaaaagttag cacaaacgtc tataacatta
    27781 ggaatggata taatagaagc gtatcaaagt agagatgctt tgatacaaga aaacgaatta
    27841 gctgtatcgt tacctgaggt cttaaaagtg agggattctg ggattgttta ttatactaag
    27901 gaaatcggaa aaactaagat agagcatctt tctcccttaa tttcttctgt cgttcaattc
    27961 atcggcttaa atatctataa aagaattact gtaaaggaaa tagctaatta tttttctgta
    28021 agtgaaacta aattaagaga aagctttaag aatgaaatgc atattacgat ttataattat
    28081 atctctaagc gaaaaatttc tacggctaaa ataatgttga aatcaaacca tactatcagt
    28141 gaagtttctt taggtttagg attttcagat tcttcacact tttcgagagt attcaaaaag
    28201 tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag tggacaattt caacaacatt
    28261 agctgagagg ttttatttta ttatgagact atatcattta cgtagtttgg aggagttgag
    28321 gaaattggat tttaacaaaa aatttttttg ggaaatttct gatttgagtt gtttcgtaga
    28381 atctgagcgt ctaagtaatg gtaataatag tgtgttaaat gaatatgaca aagtagtaat
    28441 taataaagta aaaagtttat gtgaagaagc tagagaatct atcagtcata ttaatggtag
    28501 caattttaaa caaacattat ttaatctgat taagctagat gctaaaatat caagttattt
    28561 attctttttg atacatgatg aatttatgga ttatcaaaaa ttggatcagt taattgaaag
    28621 agaaagctgg gaagattatt acgtgggtct aactttttca gaaaagttaa acaactacaa
    28681 gttaatagat tataagtatt tgagtgaaag caattaatta ttcatgtaga gtactagaca
    28741 ctttaaatat ggacaattca attataaaga aaaagaatca ataatttaat gaggctgtga
    28801 caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt
    28861 cgtttaagat tctaggaatc attcagaaat gctgtgaaac cgatacttta taattataaa
    28921 gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt
    28981 ctcattcagt aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa
    29041 tctaagaaag ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca
    29101 gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa
    29161 atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct
    29221 aagtaaatta gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga
    29281 tttatctttt attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta
    29341 gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta
    29401 tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa
    29461 caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga
    29521 taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg
    29581 gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag
    29641 acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat
    29701 caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa
    29761 aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta
    29821 atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg
    29881 gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt
    29941 ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc
    30001 gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta
    30061 ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat
    30121 ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta
    30181 atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc
    30241 ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt
    30301 atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca
    30361 tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg
    30421 atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata
    30481 atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat
    30541 atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac
    30601 aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata
    30661 cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg caagatattg
    30721 atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga
    30781 aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc
    30841 acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag
    30901 cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat
    30961 ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa
    31021 tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa
    31081 aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc
    31141 ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt
    31201 ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg
    31261 acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg
    31321 tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac
    31381 cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat aaggacttcg
    31441 gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag
    31501 gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg
    31561 taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa
    31621 acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DAFFQY010000027        30466 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFFQY010000027 DAFFQY010000000
VERSION     DAFFQY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.027"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..552)
                     /locus_tag="LRB02_001148"
     CDS             complement(<1..552)
                     /locus_tag="LRB02_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL4659364.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="LRB02_001149"
     CDS             complement(678..2606)
                     /locus_tag="LRB02_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL4659365.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="LRB02_001150"
     CDS             complement(2912..3628)
                     /locus_tag="LRB02_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL4659366.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LRB02_001151"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="LRB02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL4659367.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LRB02_001152"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="LRB02_001152"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL4659368.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="LRB02_001153"
     CDS             complement(7098..8558)
                     /locus_tag="LRB02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659369.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="LRB02_001154"
     CDS             complement(8548..9843)
                     /locus_tag="LRB02_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4659370.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="LRB02_001155"
     CDS             complement(10175..10852)
                     /locus_tag="LRB02_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL4659371.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="LRB02_001156"
     CDS             complement(10882..11973)
                     /locus_tag="LRB02_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL4659372.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="LRB02_001157"
     CDS             complement(12074..12508)
                     /locus_tag="LRB02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4659373.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LRB02_001158"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="LRB02_001158"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4659374.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16444)
                     /locus_tag="LRB02_001159"
     CDS             complement(14546..16444)
                     /locus_tag="LRB02_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4659375.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDD
                     VLSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTS
                     LNLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVS
                     AYSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFIC
                     IIVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGL
                     VPVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGI
                     TEPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVW
                     LVASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGS
                     LGEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYF
                     QSAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRK
                     LASLGGSI"
     gene            complement(16502..18277)
                     /locus_tag="LRB02_001160"
     CDS             complement(16502..18277)
                     /locus_tag="LRB02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL4659376.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18327..19823)
                     /locus_tag="LRB02_001161"
     CDS             complement(18327..19823)
                     /locus_tag="LRB02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4659377.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20017..21639)
                     /locus_tag="LRB02_001162"
     CDS             complement(20017..21639)
                     /locus_tag="LRB02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL4659378.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21967..23280)
                     /locus_tag="LRB02_001163"
     CDS             complement(21967..23280)
                     /locus_tag="LRB02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL4659379.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23525..23749)
                     /locus_tag="LRB02_001164"
     CDS             complement(23525..23749)
                     /locus_tag="LRB02_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659380.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23769..24191)
                     /locus_tag="LRB02_001165"
     CDS             complement(23769..24191)
                     /locus_tag="LRB02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL4659381.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(24333..25043)
                     /locus_tag="LRB02_001166"
     CDS             complement(24333..25043)
                     /locus_tag="LRB02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659382.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25061..25558)
                     /locus_tag="LRB02_001167"
     CDS             complement(25061..25558)
                     /locus_tag="LRB02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659383.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25595..26047)
                     /locus_tag="LRB02_001168"
     CDS             complement(25595..26047)
                     /locus_tag="LRB02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4659384.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26182..26787)
                     /locus_tag="LRB02_001169"
     CDS             complement(26182..26787)
                     /locus_tag="LRB02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4659385.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27237..27857)
                     /locus_tag="LRB02_001170"
     CDS             complement(27237..27857)
                     /locus_tag="LRB02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL4659386.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27859..28728)
                     /locus_tag="LRB02_001171"
     CDS             complement(27859..28728)
                     /locus_tag="LRB02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL4659387.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28751..29158)
                     /locus_tag="LRB02_001172"
     CDS             complement(28751..29158)
                     /locus_tag="LRB02_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL4659388.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29177..30466)
                     /locus_tag="LRB02_001173"
     CDS             complement(29177..30466)
                     /locus_tag="LRB02_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659389.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8630 a         6628 c         5022 g        10186 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    16201 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    16261 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    16321 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    16381 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    16441 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    16501 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    16561 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    16621 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    16681 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    16741 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    16801 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    16861 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    16921 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    16981 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    17041 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    17101 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    17161 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    17221 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    17281 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    17341 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    17401 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    17461 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    17521 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    17581 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    17641 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    17701 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    17761 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    17821 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    17881 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    17941 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    18001 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    18061 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    18121 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    18181 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    18241 agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac
    18301 aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta
    18361 ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag
    18421 cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc
    18481 cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa
    18541 ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt
    18601 ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact
    18661 atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga
    18721 ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa
    18781 ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga
    18841 taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct
    18901 aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc
    18961 cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat
    19021 acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata
    19081 cccatcttag catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga
    19141 aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt
    19201 ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta
    19261 ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc
    19321 tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct
    19381 accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga
    19441 tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg
    19501 tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac
    19561 tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa
    19621 agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga
    19681 atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg
    19741 tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt
    19801 tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga
    19861 agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa
    19921 aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc
    19981 gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc
    20041 ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac
    20101 aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca
    20161 aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt
    20221 cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat
    20281 caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat
    20341 ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc
    20401 acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc
    20461 caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc
    20521 aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc
    20581 ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact
    20641 ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt
    20701 attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac
    20761 ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg
    20821 atctaatcct atgtgttcga attggaagac catggataat tcatttcttt taggattgga
    20881 ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt
    20941 gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact
    21001 tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc
    21061 aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt
    21121 tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt
    21181 ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc
    21241 attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc
    21301 ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg
    21361 gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc
    21421 tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta
    21481 tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat
    21541 aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat
    21601 ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga
    21661 tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt
    21721 tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa
    21781 attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa
    21841 cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa
    21901 tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca
    21961 acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata
    22021 catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat
    22081 aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa
    22141 gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat
    22201 caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa
    22261 tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga
    22321 acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc
    22381 aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg
    22441 catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt
    22501 atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat
    22561 gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac
    22621 aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat
    22681 cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc
    22741 aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc
    22801 attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc
    22861 gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt
    22921 gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc
    22981 ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc
    23041 aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact
    23101 ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt
    23161 tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc
    23221 acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat
    23281 cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa
    23341 taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt
    23401 ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat
    23461 tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca
    23521 aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt
    23581 ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc
    23641 agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt
    23701 ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc
    23761 ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca
    23821 gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc
    23881 tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc
    23941 tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag
    24001 atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca
    24061 ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca
    24121 cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa
    24181 actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa
    24241 taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg
    24301 caatgaatgc cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg
    24361 gttagtgtgt gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct
    24421 tcgattgcct gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc
    24481 gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga
    24541 cagatcgctc cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa
    24601 tctgcaccta aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct
    24661 gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta
    24721 tagatatcta caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt
    24781 aaagcttcaa tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc
    24841 cactgagcaa tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct
    24901 tttgctgcct cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta
    24961 tcgataccat ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg
    25021 gtatccacta ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga
    25081 atagatagtc tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa
    25141 atcctttttt acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa
    25201 tggctgtttt ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga
    25261 taccttgatg tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag
    25321 ccatttcctc agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat
    25381 cgatactcac taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta
    25441 gtgcataagc ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg
    25501 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
    25561 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
    25621 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
    25681 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
    25741 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
    25801 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
    25861 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
    25921 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
    25981 attcgatatt gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt
    26041 cagccatttc atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa
    26101 atgcaactat tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
    26161 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
    26221 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
    26281 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
    26341 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
    26401 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
    26461 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
    26521 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
    26581 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa
    26641 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
    26701 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
    26761 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
    26821 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
    26881 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
    26941 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg
    27001 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
    27061 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
    27121 atgagaagct gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg
    27181 acctctcggt aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
    27241 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
    27301 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
    27361 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
    27421 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
    27481 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
    27541 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
    27601 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
    27661 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
    27721 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
    27781 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
    27841 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
    27901 cagtccaatc ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg
    27961 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
    28021 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
    28081 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
    28141 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
    28201 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
    28261 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
    28321 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
    28381 tgtcttcagg atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata
    28441 tccgctgtcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac
    28501 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
    28561 gatatattta gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc
    28621 tgcgcctagc atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
    28681 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
    28741 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
    28801 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
    28861 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
    28921 ccaaactctt ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa
    28981 gtccaacttg ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt
    29041 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
    29101 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
    29161 ttttccctac tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga
    29221 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gttgaatccc atcgaattaa
    29281 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
    29341 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
    29401 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
    29461 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat
    29521 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
    29581 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat
    29641 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
    29701 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
    29761 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
    29821 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
    29881 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
    29941 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
    30001 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
    30061 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
    30121 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
    30181 ctaaacccaa atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt
    30241 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
    30301 tcgaaccggc actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt
    30361 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
    30421 atgcctgagt caaaatattt tcgatgataa aattgataac gcccat
//
LOCUS       DAFFQY010000028        29901 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFFQY010000028 DAFFQY010000000
VERSION     DAFFQY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.028"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(287..649)
                     /locus_tag="LRB02_001174"
     CDS             complement(287..649)
                     /locus_tag="LRB02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659390.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="LRB02_001175"
     CDS             complement(723..1103)
                     /locus_tag="LRB02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL4659391.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="LRB02_001176"
     CDS             1167..2360
                     /locus_tag="LRB02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4659392.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="LRB02_001177"
     CDS             complement(2437..3021)
                     /locus_tag="LRB02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4659393.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="LRB02_001178"
     CDS             3222..4220
                     /locus_tag="LRB02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4659394.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="LRB02_001179"
     CDS             4332..4841
                     /locus_tag="LRB02_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL4659395.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="LRB02_001180"
     CDS             complement(4871..5530)
                     /locus_tag="LRB02_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL4659396.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="LRB02_001181"
     CDS             complement(5532..6296)
                     /locus_tag="LRB02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL4659397.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="LRB02_001182"
     CDS             complement(6304..6981)
                     /locus_tag="LRB02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL4659398.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..7950)
                     /locus_tag="LRB02_001183"
     CDS             complement(7018..7950)
                     /locus_tag="LRB02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBL4659399.1"
                     /translation="MGLRNPYDILPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPA
                     IDVILGSHTHHLFREGHVINDVQIAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTE
                     TMTAFTEDEQEINSYLERGHELLKEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGK
                     TEAAILNSGLFLTDIPKGRVNQDQLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNF
                     LRNYPMKGMGFRGKIFGQMVYNGITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVP
                     FFPTIEIAGKNEFLFPEFIRSVVGDYLKAHYPIK"
     gene            complement(7911..8402)
                     /locus_tag="LRB02_001184"
     CDS             complement(7911..8402)
                     /locus_tag="LRB02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL4659400.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDYGILMIF
                     CQN"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="LRB02_001185"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LRB02_001185"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="LRB02_001186"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="LRB02_001186"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL4659401.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="LRB02_001187"
     CDS             9341..10198
                     /locus_tag="LRB02_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659402.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="LRB02_001188"
     CDS             complement(10279..10614)
                     /locus_tag="LRB02_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659403.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="LRB02_001189"
     CDS             complement(10681..12051)
                     /locus_tag="LRB02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL4659404.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="LRB02_001190"
     CDS             complement(12129..12665)
                     /locus_tag="LRB02_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4659405.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="LRB02_001191"
     CDS             12974..14455
                     /locus_tag="LRB02_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659406.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14558..15265)
                     /locus_tag="LRB02_001192"
     CDS             complement(14558..15265)
                     /locus_tag="LRB02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL4659407.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15344..15682)
                     /locus_tag="LRB02_001193"
     CDS             complement(15344..15682)
                     /locus_tag="LRB02_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL4659408.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15793..16755)
                     /locus_tag="LRB02_001194"
     CDS             complement(15793..16755)
                     /locus_tag="LRB02_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL4659409.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16770..18101)
                     /locus_tag="LRB02_001195"
     CDS             complement(16770..18101)
                     /locus_tag="LRB02_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4659410.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18109..18789)
                     /locus_tag="LRB02_001196"
     CDS             complement(18109..18789)
                     /locus_tag="LRB02_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL4659411.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19008..19487)
                     /locus_tag="LRB02_001197"
     CDS             complement(19008..19487)
                     /locus_tag="LRB02_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL4659412.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIN"
     gene            complement(19614..21293)
                     /locus_tag="LRB02_001198"
     CDS             complement(19614..21293)
                     /locus_tag="LRB02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL4659413.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21841..23343
                     /locus_tag="LRB02_001199"
     CDS             21841..23343
                     /locus_tag="LRB02_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659414.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23356..24018
                     /locus_tag="LRB02_001200"
     CDS             23356..24018
                     /locus_tag="LRB02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659415.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24119..24322)
                     /locus_tag="LRB02_001201"
     CDS             complement(24119..24322)
                     /locus_tag="LRB02_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL4659416.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="LRB02_001202"
     CDS             complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="LRB02_001202"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL4659417.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="LRB02_001203"
     CDS             complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="LRB02_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL4659418.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28285..28899)
                     /locus_tag="LRB02_001204"
     CDS             complement(28285..28899)
                     /locus_tag="LRB02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4659419.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29505..29882)
                     /locus_tag="LRB02_001205"
     CDS             complement(29505..29882)
                     /locus_tag="LRB02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659420.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT         8973 a         6196 c         5004 g         9728 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaatat cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtagtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aaaaaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta
    14521 gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga
    14581 acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca
    14641 gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc
    14701 cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca
    14761 attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc
    14821 agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg
    14881 ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat
    14941 gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt
    15001 gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt
    15061 gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac
    15121 cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg
    15181 ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa
    15241 aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac tttattttat
    15301 cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa
    15361 attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc
    15421 cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt
    15481 ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg
    15541 catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt
    15601 tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt
    15661 ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt
    15721 aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt
    15781 tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc
    15841 tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg
    15901 gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg
    15961 tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat
    16021 cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag
    16081 aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata
    16141 aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc
    16201 acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt
    16261 ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc
    16321 cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact
    16381 acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg
    16441 atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga
    16501 attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg
    16561 catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc
    16621 tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc
    16681 agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc
    16741 ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg
    16801 attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt
    16861 ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc
    16921 aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt
    16981 ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca
    17041 ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca
    17101 cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc
    17161 tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct
    17221 tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga
    17281 tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc
    17341 cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc
    17401 aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc
    17461 tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag
    17521 atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc
    17581 atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca
    17641 gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca
    17701 tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc
    17761 gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc
    17821 ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca
    17881 aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt
    17941 tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct
    18001 tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa
    18061 ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag
    18121 cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc
    18181 gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg
    18241 aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg
    18301 caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc
    18361 agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc
    18421 gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa
    18481 ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac
    18541 tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc
    18601 ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca
    18661 atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc
    18721 aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt
    18781 aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat
    18841 cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac
    18901 agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata
    18961 atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta attaattaaa
    19021 tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc
    19081 ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa
    19141 aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat
    19201 cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact
    19261 tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca
    19321 gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt
    19381 tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg
    19441 atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct
    19501 ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc
    19561 aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt
    19621 ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt
    19681 tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca
    19741 aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc
    19801 atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa
    19861 atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac
    19921 aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc
    19981 attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc
    20041 acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa
    20101 tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc
    20161 atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc
    20221 tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg
    20281 atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc
    20341 ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt
    20401 cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta
    20461 acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt
    20521 cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat
    20581 acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa
    20641 tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga
    20701 ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc
    20761 aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc
    20821 gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta
    20881 tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct
    20941 accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct
    21001 aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga
    21061 agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg
    21121 ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca
    21181 ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg
    21241 ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc
    21301 acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg
    21361 agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg
    21421 aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt
    21481 cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag
    21541 ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac
    21601 aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca
    21661 tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact
    21721 atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt
    21781 aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat
    21841 atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta
    21901 ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc
    21961 gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt
    22021 gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact
    22081 gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa
    22141 aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg
    22201 gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact
    22261 actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca
    22321 gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca
    22381 gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc
    22441 attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg
    22501 agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc
    22561 gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac
    22621 acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct
    22681 gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag
    22741 ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg
    22801 gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc
    22861 ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta
    22921 ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa
    22981 gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg
    23041 aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa
    23101 gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt
    23161 caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc
    23221 aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt
    23281 tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac
    23341 taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa
    23401 gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta
    23461 ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca
    23521 aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat
    23581 cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct
    23641 ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa
    23701 gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg
    23761 accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa
    23821 catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat
    23881 atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact
    23941 catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt
    24001 caaaaaatcg aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg
    24061 tctcgcagat atggttcgga ttttttattt gcttgattca attagtaata agtggttttt
    24121 attttttttc gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt
    24181 tttctttgat gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt
    24241 ctgttgcttt gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag
    24301 aacgtccttt taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt
    24361 cagggtaaca tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt
    24421 tagagctgtt gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg
    24481 gaccattatt tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat
    24541 acacagatct gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg
    24601 tgctggatta ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac
    24661 agcttctttg atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg
    24721 attgttttca tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc
    24781 taaagaaaca tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc
    24841 tgtcttctcc caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt
    24901 ggcaaatgcc acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa
    24961 ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt
    25021 tcctccgcct aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg
    25081 agcatctgct actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga
    25141 tactgcgatt tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga
    25201 atctgttact gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc
    25261 ttctgcttcc agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc
    25321 tgttcccaaa caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac
    25381 ttctggcgtt cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca
    25441 gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc
    25501 ttcgtagccg ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac
    25561 atccattttt cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca
    25621 tttttcctct ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata
    25681 ggatctttac tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt
    25741 ttggattact tttttattta caggtctcca gtactactgc agacctattg caatggcaat
    25801 catgatgaaa atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc
    25861 ccattttgcc cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg
    25921 tgtaaaagcg tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga
    25981 tacacctact aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga
    26041 atttgatgta acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat
    26101 gacagctgga acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga
    26161 aggaacagat gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac
    26221 gattgctgta ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac
    26281 tttcattcca tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa
    26341 gaattgagaa gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca
    26401 gacaaggaca actaacatct ctaataagat caatttatgc ccaagcatga attttttatt
    26461 ttcatcagat gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga
    26521 actttctggt tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc
    26581 agtaacaact acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc
    26641 aatcccttga gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg
    26701 agatgcgaca taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa
    26761 tggaaccatc accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc
    26821 gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact
    26881 gataaacgca tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc
    26941 tccaacaaca aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac
    27001 tagtccttca aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat
    27061 tttggctgtt ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta
    27121 aagcacatca tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc
    27181 gtcagcaatg actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg
    27241 atgatcttgc agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata
    27301 acggaaggaa tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta
    27361 ttcaatatct tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa
    27421 gatgactaca ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg
    27481 tttcggtttt cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt
    27541 aattttgatg aataaaaaat ggataatcaa atgaattata cagtaaaaat gacatttcca
    27601 catgaataat ttattagaaa atcacttttt tctattttgt ttttttctcc tatgtatgat
    27661 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    27721 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    27781 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    27841 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    27901 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    27961 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    28021 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    28081 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    28141 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    28201 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    28261 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    28321 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    28381 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    28441 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    28501 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    28561 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    28621 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    28681 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    28741 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    28801 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    28861 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    28921 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    28981 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    29041 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    29101 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    29161 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    29221 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    29281 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    29341 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    29401 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    29461 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    29521 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    29581 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    29641 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    29701 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    29761 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    29821 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    29881 atagtgaaca cacacctttt t
//
LOCUS       DAFFQY010000029        26658 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFFQY010000029 DAFFQY010000000
VERSION     DAFFQY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.029"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="LRB02_001206"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="LRB02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL4659421.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="LRB02_001207"
     CDS             complement(1551..2972)
                     /locus_tag="LRB02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659422.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="LRB02_001208"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="LRB02_001208"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL4659423.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="LRB02_001209"
     CDS             complement(5383..6639)
                     /locus_tag="LRB02_001209"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL4659424.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6751..6956)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7219..8520
                     /locus_tag="LRB02_001210"
     CDS             7219..8520
                     /locus_tag="LRB02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL4659425.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="LRB02_001211"
     CDS             complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="LRB02_001211"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4659426.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(10488..11087)
                     /locus_tag="LRB02_001212"
     CDS             complement(10488..11087)
                     /locus_tag="LRB02_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4659427.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            11341..12222
                     /locus_tag="LRB02_001213"
     CDS             11341..12222
                     /locus_tag="LRB02_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659428.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            12235..13845
                     /locus_tag="LRB02_001214"
                     /pseudo
     CDS             12235..13845
                     /locus_tag="LRB02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(13922..14581)
                     /locus_tag="LRB02_001215"
     CDS             complement(13922..14581)
                     /locus_tag="LRB02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4659429.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(14864..15529)
                     /locus_tag="LRB02_001216"
     CDS             complement(14864..15529)
                     /locus_tag="LRB02_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL4659430.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="LRB02_001217"
     CDS             complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="LRB02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL4659431.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(16309..17319)
                     /gene="dhaK"
                     /locus_tag="LRB02_001218"
     CDS             complement(16309..17319)
                     /gene="dhaK"
                     /locus_tag="LRB02_001218"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL4659432.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(17294..17740)
                     /gene="dhaM"
                     /locus_tag="LRB02_001219"
     CDS             complement(17294..17740)
                     /gene="dhaM"
                     /locus_tag="LRB02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL4659433.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            17843..18163
                     /locus_tag="LRB02_001220"
     CDS             17843..18163
                     /locus_tag="LRB02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL4659434.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(18275..19144)
                     /locus_tag="LRB02_001221"
     CDS             complement(18275..19144)
                     /locus_tag="LRB02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4659435.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(19240..20544)
                     /locus_tag="LRB02_001222"
     CDS             complement(19240..20544)
                     /locus_tag="LRB02_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4659436.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(20541..21050)
                     /locus_tag="LRB02_001223"
     CDS             complement(20541..21050)
                     /locus_tag="LRB02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659437.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(21225..23360)
                     /locus_tag="LRB02_001224"
     CDS             complement(21225..23360)
                     /locus_tag="LRB02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4659438.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(23443..25098)
                     /locus_tag="LRB02_001225"
     CDS             complement(23443..25098)
                     /locus_tag="LRB02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4659439.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(25100..25414)
                     /locus_tag="LRB02_001226"
     CDS             complement(25100..25414)
                     /locus_tag="LRB02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL4659440.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25584..25844
                     /locus_tag="LRB02_001227"
     CDS             25584..25844
                     /locus_tag="LRB02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL4659441.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            25858..26172
                     /locus_tag="LRB02_001228"
     CDS             25858..26172
                     /locus_tag="LRB02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL4659442.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         7493 a         5564 c         4829 g         8772 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaatc gagtagaggg gaaagccaac tgaccttccg cccctctcac
     8581 accaccgtac gtacggaccc gtatacggcg gttcaataag ttgagtgtac gaactggtac
     8641 ttcaaggaga tgtcctcata tccttggcgg gccagttctt tgtctgttaa tgatcgatga
     8701 agaatatgac ttttggagac tgaccaaata ccttttcttg tatttgacca ttcatacgct
     8761 ttcgctttat cgatccctaa acgaatcaga ttttttcttt tggttcttgg attcttccat
     8821 tgtttccaaa tatattgtct gattcttcgc ttcaaccaac cgtttaactc cctcatgaat
     8881 tctttcatct caccaattcc atagtagttg atccaaccag tcattagttg cttgatttct
     8941 tttaaaataa gttggatact tcttccacgg tttcttttag tgagttgttt gagcttgctt
     9001 ttcacacgct gttttgcttt tgcgtgaggg cgaattttaa tacctttctt tgttggcaga
     9061 atgcagaatc caagaaactt tcgtttcaat ggacttccta ccgcactctt ttctcgattt
     9121 accgtcaact ttagttgttc ttcgagaaat tgaatggatt tcttcatcac acgctgtcct
     9181 gctcgtttgc ttctaacata gatattgcag tcatcggcgt atcgaacaaa tttatgtcct
     9241 agattttcta gcacttgatc aaattcgttc aggtagatgt tacttaacag gggagagaga
     9301 ttgcctcctt ggggcgttcc ttcttcagtt gttgtgatga gtccattttc cattacacca
     9361 ctcattaagt attgacggat cagttttagg acttgtttgt cctttacttt ccgctcaatg
     9421 aaatacatca gcttatcgtg atttacggtg tcaaagtagg ctttcatatc aatgtccacc
     9481 acatatcggt aaccttgttc ataataagat tttgctttct tcactgcgtc atgtgcgctg
     9541 cgatttgggc gaaacccata actattatct gagaattctg gttcaaatag cggttgtaac
     9601 acttgattga ttgcctgttg aagcatacga tcaataaccg tgggaattcc tagttttctt
     9661 acaccgccgt ttggtttcgg tatttctact cgcaaaacag gctgaggttt atagttccct
     9721 tggcgtaaat tggatagtaa ttcctcgcgg tgctctttca agtaaggaag cagttgatcg
     9781 acggtcatcc catctgcacc agccgcccct ttatttctga ctacttgaag atacgcttga
     9841 ttgagattat tccgatctag aattctccca aacagatttt ctacaccatc ttgtttcctc
     9901 tgttcaccgc aagaagtgct gcgcacttca tcataccctt cgtgttccac actagctctt
     9961 tgatgatagc caagttttct tgttttctgc ggtcgtcgca tcttctcaca cctcctaaat
    10021 tattcaagat tcttattgtt cagtccttcg taacggtcag tgtcgttact actatgacct
    10081 cggctgactt ctcatgattc accatcatgt cgccatgatc gttgtgctgg tttgttttca
    10141 ttcaacgccc acaacaccct catgagatct cccccggtaa gagtgaaaac tttcctccca
    10201 tgtaactgct agatttactg tataggtttc gggcagtatt ggactttgtc ttgtgatgaa
    10261 gacttatccg acctaattca gccttgtatc tagtttctgt tcgtcagttc aggagtttgc
    10321 gtccgacttc cttcagccac aacctcacga ttgccgcctt gtctttcgct aacagttcct
    10381 actgccaagc ctgtagtgga ctttcaccac caagttttca cccatgcagg gcgcacacca
    10441 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    10501 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    10561 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    10621 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    10681 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    10741 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    10801 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    10861 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    10921 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    10981 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    11041 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    11101 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    11161 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    11221 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
    11281 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
    11341 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
    11401 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
    11461 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
    11521 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
    11581 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
    11641 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
    11701 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
    11761 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
    11821 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    11881 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    11941 gtgatcatac gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg
    12001 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    12061 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    12121 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    12181 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    12241 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    12301 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    12361 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    12421 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    12481 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    12541 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    12601 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    12661 cttttagcaa tatcgatcag ttgtacgatg atttttttcc aagcggattc gatcactgca
    12721 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    12781 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    12841 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    12901 atgtggaaac ctttaggctg gtcttatttg acctatccat ttacaactaa caactgaatt
    12961 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    13021 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    13081 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    13141 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    13201 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    13261 gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca
    13321 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    13381 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    13441 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    13501 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    13561 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt
    13621 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    13681 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    13741 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    13801 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    13861 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    13921 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    13981 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    14041 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    14101 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    14161 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    14221 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    14281 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    14341 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    14401 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    14461 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    14521 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    14581 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    14641 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    14701 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    14761 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    14821 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    14881 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    14941 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    15001 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    15061 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    15121 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    15181 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    15241 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    15301 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    15361 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    15421 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    15481 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    15541 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    15601 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    15661 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    15721 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    15781 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    15841 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    15901 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    15961 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    16021 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    16081 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    16141 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    16201 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    16261 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    16321 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    16381 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    16441 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    16501 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    16561 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    16621 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    16681 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    16741 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    16801 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    16861 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    16921 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    16981 attcttaacg atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat
    17041 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    17101 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    17161 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    17221 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    17281 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    17341 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    17401 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    17461 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    17521 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    17581 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    17641 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    17701 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    17761 taataaattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
    17821 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    17881 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    17941 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    18001 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    18061 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    18121 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    18181 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    18241 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    18301 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    18361 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    18421 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    18481 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    18541 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    18601 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    18661 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    18721 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    18781 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    18841 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    18901 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    18961 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
    19021 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    19081 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    19141 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    19201 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    19261 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    19321 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    19381 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    19441 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    19501 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    19561 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    19621 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    19681 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggaa cttccatgaa
    19741 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    19801 actgatttct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    19861 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    19921 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    19981 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    20041 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    20101 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    20161 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    20221 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    20281 attcaatttc gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc
    20341 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    20401 cttatgtttg tatataaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    20461 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
    20521 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    20581 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    20641 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    20701 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    20761 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    20821 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    20881 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    20941 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    21001 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    21061 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    21121 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    21181 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    21241 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    21301 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    21361 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    21421 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    21481 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    21541 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    21601 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    21661 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    21721 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    21781 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    21841 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    21901 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    21961 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    22021 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    22081 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    22141 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    22201 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    22261 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    22321 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    22381 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    22441 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    22501 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    22561 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    22621 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    22681 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    22741 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    22801 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    22861 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    22921 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    22981 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    23041 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    23101 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    23161 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    23221 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    23281 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    23341 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg taccgattag
    23401 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    23461 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    23521 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    23581 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    23641 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    23701 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    23761 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    23821 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    23881 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    23941 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    24001 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    24061 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    24121 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    24181 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    24241 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    24301 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    24361 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    24421 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    24481 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    24541 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    24601 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    24661 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    24721 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    24781 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    24841 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    24901 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    24961 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    25021 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    25081 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    25141 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    25201 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    25261 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    25321 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    25381 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    25441 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    25501 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    25561 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    25621 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    25681 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    25741 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    25801 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    25861 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    25921 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    25981 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    26041 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    26101 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    26161 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    26221 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    26281 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg
    26341 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    26401 gtttgaccta agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt
    26461 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    26521 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggtttggt
    26581 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    26641 aggtcacacc cctttttt
//
LOCUS       DAFFQY010000030        26351 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFFQY010000030 DAFFQY010000000
VERSION     DAFFQY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.030"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(98..1228)
                     /locus_tag="LRB02_001229"
     CDS             complement(98..1228)
                     /locus_tag="LRB02_001229"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL4659443.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="LRB02_001230"
     CDS             complement(1877..3079)
                     /locus_tag="LRB02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4659444.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="LRB02_001231"
     CDS             complement(3139..3342)
                     /locus_tag="LRB02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL4659445.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="LRB02_001232"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="LRB02_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="LRB02_001233"
     CDS             4879..5691
                     /locus_tag="LRB02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659446.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6714..6866)
                     /locus_tag="LRB02_001234"
     CDS             complement(6714..6866)
                     /locus_tag="LRB02_001234"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL4659447.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7057..7935)
                     /locus_tag="LRB02_001235"
     CDS             complement(7057..7935)
                     /locus_tag="LRB02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4659448.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7974..9092)
                     /locus_tag="LRB02_001236"
     CDS             complement(7974..9092)
                     /locus_tag="LRB02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL4659449.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9089..11188)
                     /locus_tag="LRB02_001237"
     CDS             complement(9089..11188)
                     /locus_tag="LRB02_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL4659450.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11239..12060)
                     /locus_tag="LRB02_001238"
     CDS             complement(11239..12060)
                     /locus_tag="LRB02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4659451.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12057..12866)
                     /locus_tag="LRB02_001239"
     CDS             complement(12057..12866)
                     /locus_tag="LRB02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659452.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12887..13381)
                     /locus_tag="LRB02_001240"
     CDS             complement(12887..13381)
                     /locus_tag="LRB02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4659453.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13393..13881)
                     /locus_tag="LRB02_001241"
     CDS             complement(13393..13881)
                     /locus_tag="LRB02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4659454.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13958..16654)
                     /locus_tag="LRB02_001242"
     CDS             complement(13958..16654)
                     /locus_tag="LRB02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4659455.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17082..18155)
                     /locus_tag="LRB02_001243"
     CDS             complement(17082..18155)
                     /locus_tag="LRB02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4659456.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18152..18979)
                     /locus_tag="LRB02_001244"
     CDS             complement(18152..18979)
                     /locus_tag="LRB02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659457.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18980..19786)
                     /locus_tag="LRB02_001245"
     CDS             complement(18980..19786)
                     /locus_tag="LRB02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659458.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19798..20883)
                     /locus_tag="LRB02_001246"
     CDS             complement(19798..20883)
                     /locus_tag="LRB02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659459.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20900..21442)
                     /locus_tag="LRB02_001247"
     CDS             complement(20900..21442)
                     /locus_tag="LRB02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659460.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21700..22689
                     /locus_tag="LRB02_001248"
     CDS             21700..22689
                     /locus_tag="LRB02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL4659461.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22686..23423
                     /locus_tag="LRB02_001249"
     CDS             22686..23423
                     /locus_tag="LRB02_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL4659462.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23420..23971
                     /gene="coaC"
                     /locus_tag="LRB02_001250"
     CDS             23420..23971
                     /gene="coaC"
                     /locus_tag="LRB02_001250"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL4659463.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23987..24619
                     /locus_tag="LRB02_001251"
     CDS             23987..24619
                     /locus_tag="LRB02_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4659464.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24704..25351
                     /locus_tag="LRB02_001252"
     CDS             24704..25351
                     /locus_tag="LRB02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4659465.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25356..26339
                     /locus_tag="LRB02_001253"
     CDS             25356..26339
                     /locus_tag="LRB02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL4659466.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8059 a         5571 c         4466 g         8255 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt tcttgctatc tttttataat
     6661 cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa
     6721 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
     6781 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
     6841 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
     6901 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
     6961 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
     7021 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
     7081 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
     7141 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
     7201 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
     7261 tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
     7321 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt tttcaatcgc
     7381 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
     7441 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
     7501 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
     7561 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
     7621 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
     7681 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     7741 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     7801 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     7861 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     7921 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     7981 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     8041 cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     8101 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     8161 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     8221 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     8281 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     8341 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     8401 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     8461 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     8521 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     8581 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     8641 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     8701 ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc tccaaaccca
     8761 ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc atcccaataa
     8821 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     8881 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     8941 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     9001 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     9061 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg tgaatcgttt
     9121 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     9181 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     9241 tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     9301 tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc gatgcccagc
     9361 cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct aagcgatacg
     9421 gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct aatcgggtta
     9481 cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca ggaaaagtgg
     9541 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt ggattgaaag
     9601 aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct aaaaattgat
     9661 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttaatttttc
     9721 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     9781 gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat tgctcaccaa
     9841 aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag gcaatgtttt
     9901 tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca agtccaatac
     9961 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
    10021 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
    10081 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
    10141 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
    10201 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
    10261 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
    10321 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
    10381 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
    10441 ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa tagttcaatg
    10501 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
    10561 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
    10621 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga tacgaaggca
    10681 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
    10741 caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca atgaccttaa
    10801 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
    10861 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
    10921 aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
    10981 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct
    11041 tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga tagtagctgt
    11101 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg
    11161 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
    11221 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
    11281 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagctcaa
    11341 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
    11401 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
    11461 cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
    11521 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
    11581 atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa ttggtgccaa
    11641 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
    11701 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
    11761 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
    11821 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
    11881 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
    11941 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg
    12001 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
    12061 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
    12121 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
    12181 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
    12241 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
    12301 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
    12361 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
    12421 taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc tttttgatca
    12481 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
    12541 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
    12601 acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa agaaggctcc
    12661 gccgtactag agattccgat attgacgaca cccataaaga tggcttctag tgacgccccg
    12721 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
    12781 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
    12841 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
    12901 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
    12961 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
    13021 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
    13081 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
    13141 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
    13201 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
    13261 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
    13321 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
    13381 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
    13441 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
    13501 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
    13561 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
    13621 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
    13681 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
    13741 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
    13801 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
    13861 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
    13921 acctaaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
    13981 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
    14041 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
    14101 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
    14161 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
    14221 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
    14281 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
    14341 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
    14401 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
    14461 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
    14521 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
    14581 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
    14641 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
    14701 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
    14761 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
    14821 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
    14881 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
    14941 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
    15001 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
    15061 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
    15121 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
    15181 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
    15241 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
    15301 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
    15361 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
    15421 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
    15481 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
    15541 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
    15601 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
    15661 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
    15721 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
    15781 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
    15841 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
    15901 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
    15961 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
    16021 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
    16081 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
    16141 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
    16201 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
    16261 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
    16321 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
    16381 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
    16441 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
    16501 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
    16561 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
    16621 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
    16681 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    16741 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    16801 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    16861 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    16921 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    16981 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    17041 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    17101 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    17161 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    17221 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    17281 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    17341 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    17401 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    17461 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    17521 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    17581 ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc
    17641 agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt
    17701 gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa
    17761 tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct
    17821 ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata
    17881 tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc
    17941 gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc
    18001 gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca
    18061 ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg
    18121 agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta
    18181 ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa
    18241 tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga
    18301 ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga
    18361 cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta
    18421 tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac
    18481 caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca
    18541 tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta
    18601 atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag
    18661 agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt
    18721 aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag
    18781 ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat
    18841 aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa
    18901 aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg
    18961 accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca
    19021 tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt
    19081 gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa
    19141 tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt
    19201 tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc
    19261 ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg
    19321 gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga
    19381 ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca
    19441 cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa
    19501 taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt
    19561 aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca
    19621 tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat
    19681 tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga
    19741 gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta
    19801 ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac
    19861 atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt
    19921 tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat aatggacacc
    19981 acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt
    20041 caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac
    20101 acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac
    20161 gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc
    20221 tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat
    20281 tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg
    20341 cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg
    20401 ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg
    20461 tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat
    20521 acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa
    20581 tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac
    20641 tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat
    20701 gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca
    20761 gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt
    20821 taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc
    20881 cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc
    20941 gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc
    21001 ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa
    21061 aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag
    21121 tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat
    21181 acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat
    21241 tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa
    21301 agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg
    21361 acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc
    21421 ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc
    21481 ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa
    21541 attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta
    21601 tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat
    21661 ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt
    21721 caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg
    21781 gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg
    21841 gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg gattgattta
    21901 tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct
    21961 actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca
    22021 ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt
    22081 acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc
    22141 gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg
    22201 ttacataaac taggattcca gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca
    22261 tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca
    22321 acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag
    22381 ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct
    22441 gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt
    22501 ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg
    22561 gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa
    22621 acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac
    22681 cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg
    22741 ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca
    22801 atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact
    22861 ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac
    22921 taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc
    22981 ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag
    23041 aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct
    23101 cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc
    23161 tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa
    23221 aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt
    23281 tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag
    23341 atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa
    23401 ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc
    23461 ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat
    23521 tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg
    23581 cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact
    23641 agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct
    23701 agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac
    23761 accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg
    23821 taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt
    23881 agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacggtca tcgaacaggt
    23941 catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa
    24001 aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt
    24061 aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc
    24121 ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag
    24181 catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc
    24241 agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat
    24301 cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa
    24361 agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt
    24421 agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa
    24481 tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg
    24541 aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca
    24601 cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg
    24661 ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt
    24721 aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga
    24781 aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc
    24841 gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat
    24901 cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga
    24961 tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt
    25021 cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat
    25081 tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca
    25141 accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga
    25201 gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga
    25261 agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac
    25321 agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc
    25381 ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg
    25441 aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat
    25501 cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg
    25561 agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc
    25621 taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata
    25681 cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac
    25741 tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg
    25801 gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta
    25861 ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca
    25921 actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg
    25981 atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg
    26041 ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc
    26101 ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg
    26161 atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa
    26221 atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc
    26281 atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa
    26341 atcatgctga t
//
LOCUS       DAFFQY010000031        25943 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFFQY010000031 DAFFQY010000000
VERSION     DAFFQY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.031"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="LRB02_001254"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="LRB02_001254"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL4659467.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMDVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="LRB02_001255"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="LRB02_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL4659468.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="LRB02_001256"
     CDS             complement(3549..4358)
                     /locus_tag="LRB02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4659469.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="LRB02_001257"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="LRB02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL4659470.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="LRB02_001258"
     CDS             complement(7968..8654)
                     /locus_tag="LRB02_001258"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL4659471.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="LRB02_001259"
     CDS             complement(9002..10783)
                     /locus_tag="LRB02_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4659472.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="LRB02_001260"
     CDS             complement(10809..11723)
                     /locus_tag="LRB02_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659473.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="LRB02_001261"
     CDS             complement(11750..12712)
                     /locus_tag="LRB02_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659474.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="LRB02_001262"
     CDS             complement(12712..13656)
                     /locus_tag="LRB02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659475.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="LRB02_001263"
     CDS             complement(13657..14661)
                     /locus_tag="LRB02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659476.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="LRB02_001264"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="LRB02_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL4659477.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="LRB02_001265"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="LRB02_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL4659478.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="LRB02_001266"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="LRB02_001266"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL4659479.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="LRB02_001267"
     CDS             complement(18496..20178)
                     /locus_tag="LRB02_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL4659480.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="LRB02_001268"
     CDS             complement(20230..20592)
                     /locus_tag="LRB02_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4659481.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="LRB02_001269"
     CDS             20862..21050
                     /locus_tag="LRB02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL4659482.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="LRB02_001270"
     CDS             21288..23957
                     /locus_tag="LRB02_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL4659483.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="LRB02_001271"
     CDS             complement(24048..24668)
                     /locus_tag="LRB02_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4659484.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="LRB02_001272"
     CDS             24780..25181
                     /locus_tag="LRB02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659485.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="LRB02_001273"
     CDS             25316..25654
                     /locus_tag="LRB02_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBL4659486.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7026 a         5500 c         4644 g         8773 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatc
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttc
//
LOCUS       DAFFQY010000032        25710 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFFQY010000032 DAFFQY010000000
VERSION     DAFFQY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.032"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(33..752)
                     /locus_tag="LRB02_001274"
     CDS             complement(33..752)
                     /locus_tag="LRB02_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659487.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(883..2034)
                     /locus_tag="LRB02_001275"
     CDS             complement(883..2034)
                     /locus_tag="LRB02_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL4659488.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2251..2991)
                     /locus_tag="LRB02_001276"
     CDS             complement(2251..2991)
                     /locus_tag="LRB02_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4659489.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(2995..3318)
                     /gene="rsfS"
                     /locus_tag="LRB02_001277"
     CDS             complement(2995..3318)
                     /gene="rsfS"
                     /locus_tag="LRB02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL4659490.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3375..3968)
                     /gene="yqeK"
                     /locus_tag="LRB02_001278"
     CDS             complement(3375..3968)
                     /gene="yqeK"
                     /locus_tag="LRB02_001278"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL4659491.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3952..4602)
                     /locus_tag="LRB02_001279"
     CDS             complement(3952..4602)
                     /locus_tag="LRB02_001279"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL4659492.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4612..4923)
                     /gene="yhbY"
                     /locus_tag="LRB02_001280"
     CDS             complement(4612..4923)
                     /gene="yhbY"
                     /locus_tag="LRB02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL4659493.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4939..6048)
                     /gene="yqeH"
                     /locus_tag="LRB02_001281"
     CDS             complement(4939..6048)
                     /gene="yqeH"
                     /locus_tag="LRB02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL4659494.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6045..6572)
                     /locus_tag="LRB02_001282"
     CDS             complement(6045..6572)
                     /locus_tag="LRB02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL4659495.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6752..7537)
                     /locus_tag="LRB02_001283"
     CDS             complement(6752..7537)
                     /locus_tag="LRB02_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL4659496.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7530..8399)
                     /locus_tag="LRB02_001284"
     CDS             complement(7530..8399)
                     /locus_tag="LRB02_001284"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL4659497.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8400..9779)
                     /locus_tag="LRB02_001285"
     CDS             complement(8400..9779)
                     /locus_tag="LRB02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL4659498.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9787..10206)
                     /gene="fabZ"
                     /locus_tag="LRB02_001286"
     CDS             complement(9787..10206)
                     /gene="fabZ"
                     /locus_tag="LRB02_001286"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL4659499.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10231..10707)
                     /gene="accB"
                     /locus_tag="LRB02_001287"
     CDS             complement(10231..10707)
                     /gene="accB"
                     /locus_tag="LRB02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL4659500.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10710..11945)
                     /gene="fabF"
                     /locus_tag="LRB02_001288"
     CDS             complement(10710..11945)
                     /gene="fabF"
                     /locus_tag="LRB02_001288"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL4659501.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(11964..12701)
                     /gene="fabG"
                     /locus_tag="LRB02_001289"
     CDS             complement(11964..12701)
                     /gene="fabG"
                     /locus_tag="LRB02_001289"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL4659502.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12713..13630)
                     /gene="fabD"
                     /locus_tag="LRB02_001290"
     CDS             complement(12713..13630)
                     /gene="fabD"
                     /locus_tag="LRB02_001290"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL4659503.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13737..13961)
                     /locus_tag="LRB02_001291"
     CDS             complement(13737..13961)
                     /locus_tag="LRB02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL4659504.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14014..14976)
                     /locus_tag="LRB02_001292"
     CDS             complement(14014..14976)
                     /locus_tag="LRB02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL4659505.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(14986..15426)
                     /locus_tag="LRB02_001293"
     CDS             complement(14986..15426)
                     /locus_tag="LRB02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4659506.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15732..16697
                     /locus_tag="LRB02_001294"
     CDS             15732..16697
                     /locus_tag="LRB02_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4659507.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16754..16984)
                     /locus_tag="LRB02_001295"
     CDS             complement(16754..16984)
                     /locus_tag="LRB02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL4659508.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17070..17957)
                     /locus_tag="LRB02_001296"
     CDS             complement(17070..17957)
                     /locus_tag="LRB02_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL4659509.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18046..19269)
                     /locus_tag="LRB02_001297"
     CDS             complement(18046..19269)
                     /locus_tag="LRB02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4659510.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19286..20332)
                     /locus_tag="LRB02_001298"
     CDS             complement(19286..20332)
                     /locus_tag="LRB02_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4659511.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(20496..22223)
                     /gene="ptsP"
                     /locus_tag="LRB02_001299"
     CDS             complement(20496..22223)
                     /gene="ptsP"
                     /locus_tag="LRB02_001299"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL4659512.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22223..22489)
                     /locus_tag="LRB02_001300"
     CDS             complement(22223..22489)
                     /locus_tag="LRB02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL4659513.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            22968..25202
                     /locus_tag="LRB02_001301"
     CDS             22968..25202
                     /locus_tag="LRB02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4659514.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25350..25670
                     /locus_tag="LRB02_001302"
     CDS             25350..25670
                     /locus_tag="LRB02_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL4659515.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         7154 a         5598 c         4455 g         8503 t
ORIGIN      
        1 aagactgaat acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac
       61 acatgctgta catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga
      121 tcttctggag atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa
      181 tcagtgattc ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg
      241 taaacgatga tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt
      301 aggtattcaa ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca
      361 aacatgtaag aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc
      421 cgtacgtctg ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag
      481 ccgcttggtc caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt
      541 gctttttcga ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca
      601 atgacgaaac gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg
      661 tagatttcaa tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc
      721 tttgctacga tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag
      781 tttcataacg caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt
      841 tttttctttc ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat
      901 ttgcccgaaa ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac
      961 taacagttcc gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt
     1021 tttttctttt aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt
     1081 attttgttgg acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg
     1141 ttggatccgt gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact
     1201 atcagcagtt gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat
     1261 atctgtcagt tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga
     1321 ttcccaagtc tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc
     1381 agctattctt tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg
     1441 atctgtgatg attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat
     1501 agggtggaca tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga
     1561 agaaaaatca agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg
     1621 ataactatac gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc
     1681 ttggacaaag cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca
     1741 caaactgtca cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc
     1801 tgactgaacc gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc
     1861 ttcattcgcg cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc
     1921 gctcattaca gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata
     1981 gcgatgtcca ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat
     2041 cctccttcat agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg
     2101 aaaaaatacc cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc
     2161 tgcactgcaa gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc
     2221 ttttcttgtt aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg
     2281 ctttgatttc tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa
     2341 gccgctgttt tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag
     2401 ttcatcttca cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa
     2461 atgttcgatg ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc
     2521 cccttgataa tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac
     2581 gtcaaataaa aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg
     2641 tacctcttgt ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata
     2701 ttgtccgatt tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc
     2761 ttcatatgct cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac
     2821 tttatatcca tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa
     2881 aatatttttt gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag
     2941 tgtttcgtcc atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc
     3001 caaccattca ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa
     3061 atttcgttct ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca
     3121 ttttccgcct tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc
     3181 aatgatttct tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa
     3241 ataatctgcc aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt
     3301 tgaatctgct gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc
     3361 ctaatatgag cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga
     3421 taaatctgtt gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca
     3481 gcttcgtcta gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga
     3541 cctggttcaa tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc
     3601 ccagtcgtat gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc
     3661 tctacaaacg aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc
     3721 ggatcaaaac cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca
     3781 taatcatgtg tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca
     3841 agagccacgg ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc
     3901 atctgtattt tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc
     3961 atattcatac aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg
     4021 gatagaacag ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg
     4081 tacatctacc caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc
     4141 cacaaagttg accatagaag tcagctcatc gattttatac cattttggca aatattctac
     4201 catatcccca ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt
     4261 catagtatca taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa
     4321 aaaaggattg tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt
     4381 ctgtttctca tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc
     4441 taaattccgg ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt
     4501 tccgcccaag atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa
     4561 taattgtgaa cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc
     4621 ttttacttct tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat
     4681 aacacgtcca atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc
     4741 ttcttctgca atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc
     4801 taaagcttct tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa
     4861 aatcggctga agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact
     4921 cataattttc cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg
     4981 cgcccagccc gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac
     5041 gatgtctgtt ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac
     5101 ttcatcagga cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc
     5161 tttttccagt ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc
     5221 acgattgccg ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc
     5281 attcagctga taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt
     5341 tttacctaag tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa
     5401 atgcccgtca tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt
     5461 tgtgattaca tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt
     5521 tccgacattt gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt
     5581 ttctaatagt tccgtcatct catgagattt cttagcacta gttaataata catctactgg
     5641 acgaattcct gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa
     5701 tgatttaggt aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa
     5761 acggtgtagt cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat
     5821 caatgcatct ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga
     5881 gacatcttgg atctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc
     5941 ggaagctaaa cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt
     6001 tgtttgtata acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct
     6061 cgccagccca tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa
     6121 cgattaaatt gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt
     6181 atccctgcac gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc
     6241 tcatcatctg ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc
     6301 attgctcgtg caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa
     6361 ttattgtttg aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta
     6421 agcaactctt ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc
     6481 aatactgctt tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct
     6541 accatccatg ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag
     6601 gagcacataa agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct
     6661 ctatactcgt tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt
     6721 gatcacagaa aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt
     6781 tttccagtaa tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt
     6841 ttttcataat gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa
     6901 taactcgatc aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag
     6961 ctgcttctgc ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa
     7021 gcacagcata aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg
     7081 caccgccact tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca
     7141 tttcgagcag atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc
     7201 aatatgcacc tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt
     7261 tcatcagtcg taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga
     7321 tattttcctg cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta
     7381 atcgagcgat tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt
     7441 cctcgaaaga atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc
     7501 ttgctaactg aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta
     7561 tgcaattcaa gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg
     7621 acaaaaccat gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg
     7681 atcgtctgtt cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga
     7741 ataatatcgc cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa
     7801 acagttagat aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc
     7861 atctgcatta gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag
     7921 acgactggaa gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca
     7981 atcgttccca tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc
     8041 ccctgaatgg ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt
     8101 tttttaattt tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta
     8161 tcccattctt caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt
     8221 cgaaaagcgt atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg
     8281 atatgtttgc aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca
     8341 ggtttgctct ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc
     8401 tatgcgcctc ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat
     8461 gctgtgctgt aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct
     8521 gcgttcgtga tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat
     8581 gcttcaaaac gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc
     8641 gggatcgtat atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga
     8701 agcagtaagt tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca
     8761 ttaatccggc attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa
     8821 ggctcaccgg ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct
     8881 gttactggat gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct
     8941 tcatccatca gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga
     9001 acagcagcgt ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct
     9061 tccagtactt tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca
     9121 tgaccaaatc gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt
     9181 tccaaataca tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa
     9241 tgctgtggga gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca
     9301 gctgctttca gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact
     9361 gtagataata caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga
     9421 cgcgcattga tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg
     9481 atattgcact cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct
     9541 ggatgaattg cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga
     9601 tatgaatcaa cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga
     9661 agtgcttcgg catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat
     9721 gcacgaatga tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata
     9781 tttcacctat ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt
     9841 agcgattcct ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta
     9901 taaggtatct cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata
     9961 agccgtcttt cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat
    10021 tgcttcaaca atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa
    10081 gggctcattg atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc
    10141 cactcgatca atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat
    10201 gttcattgtt ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg
    10261 tccatattcg acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc
    10321 actagttatc tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc
    10381 aacttgatca cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat
    10441 tcccacgatt ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat
    10501 tatttctgga gcagttgttg cttgttcaga tatgacattg actggctccg aaactggcaa
    10561 cgtttcgttt gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa
    10621 ttcaaattga ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac
    10681 taattctctg atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc
    10741 gcattatgtc cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg
    10801 ctttcgtttt tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta
    10861 ggtgggataa attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct
    10921 gctcccaata aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt
    10981 tcacctaatg cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt
    11041 ccgtgagcat tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc
    11101 ttcattgctt tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca
    11161 cagtttgcac cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt
    11221 tctaaagatt caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt
    11281 tcgtcaaacg gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct
    11341 gcaaatccag caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca
    11401 tcagaatagc catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca
    11461 caagctgtta cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca
    11521 gctgccatgt tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt
    11581 tcatgtagac gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg
    11641 atgacgccaa accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct
    11701 tctaaagcag cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta
    11761 ataaaatatt tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct
    11821 gaagcatcaa atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa
    11881 aagctgtctg cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga
    11941 ttcatttgtt tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg
    12001 acttgtccgg tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct
    12061 acttgtccga aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct
    12121 aaaacttccg tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct
    12181 ctagttgcag cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct
    12241 gcataatttg cttgaccaat atttccaatc aacccagata cactagagag attaatgatg
    12301 gccccttctc gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca
    12361 atcaaattga tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg
    12421 tcatttgtga ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct
    12481 tctttgatca tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat
    12541 tttacaccga atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc
    12601 aaaatgatat ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt
    12661 gtactacctg tgataaatac attttttcct tttacatcca taacgttcct ccttatagta
    12721 ttgctattgt ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat
    12781 cgattttttt cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga
    12841 tgccgattgt ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct
    12901 gttcaagtag tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga
    12961 taggaaaaga aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg
    13021 ccggttctaa aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga
    13081 accctttttc ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca
    13141 cgatctgctg aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat
    13201 gacagctttc ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc
    13261 cagctggggt agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag
    13321 tgaaggatag cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag
    13381 ctgcatctgg gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta
    13441 gaatagctgg ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt
    13501 cagccatatc gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt
    13561 cttcatataa atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg
    13621 ctgttttcat gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa
    13681 aggccgagac attcctattt caggaaatgt cccagccccc aacctaattt taatgattat
    13741 gcagtttgtt tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt
    13801 tcgattttca cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg
    13861 tcagcttcta agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct
    13921 aattcttctg cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat
    13981 attgtttgct ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca
    14041 tgttaaacct ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt
    14101 tccactagaa actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata
    14161 tttatccata ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc
    14221 aataattctg aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac
    14281 ttcttggata ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat
    14341 ttgcaaataa tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta
    14401 gccagcggtc aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt
    14461 tttctgtgga ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt
    14521 cgtacgatca gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt
    14581 agttgagccg cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca
    14641 cgcagcactg atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc
    14701 tgctacagat ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc
    14761 tttagggttt agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga
    14821 agtctcctga tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc
    14881 atcagaggta tccatcatct tggaaagatc attattagta ataatctttt caggaaggac
    14941 gctggctgtt gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg
    15001 caagaagtcg tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat
    15061 tccgtctaag atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa
    15121 ctttcctttt ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg
    15181 ttcgacatag ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat
    15241 tgatagttct ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat
    15301 cgtgtgcatc tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc
    15361 gattgtcaaa atatcattga agacactaac aagataatca tttactgttt ttaaatctgg
    15421 ttccatgttc caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat
    15481 atcttaactt ttttcaaaat gcaaccttaa aagatcattt ttttactata tgagcttttt
    15541 ttaagatttt actcatttaa aacaactgat aaatcagcat ttataatttc ttttattaga
    15601 aaaatcatct atctcacata ttttgattat caaagtattt tctgtgaaca gaactataga
    15661 attccctaaa acttgtccat tatttcaaaa tttgttatag tctttttatt caaagcgtaa
    15721 aaggagatcc tatgttaaaa gaaaaaatga aaactctccc catgctgccg ggtgtctatc
    15781 ttatgaaaga ccgagatggt aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata
    15841 gagtaagcag ctattttcat cagaataaac agcattcaaa aaaagtatta cgtatgattc
    15901 atcatataac agattttgat ttcgtcgtag tagacacgga attagatgct ttattgctcg
    15961 agtgtcaatt gatccaacat taccgtcctt tttataaccg acaaatgaat tatttcagta
    16021 attataatta tgtacatatt accaataagg gatttgtatt aacagatact cctacggctc
    16081 gaacatacgg gccttttcgt ctctataaaa aaatgccgtc tattttgcgt ataatggaag
    16141 aaacttatca aatgccttgg ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc
    16201 ctgacctaca ggaaatgact tttgaacaga aaaaaaagga actccaaggg ctgtttcaag
    16261 gccgaaataa aaagctactg acttacttga aaaaaagaca acaacatttc atctatcagt
    16321 tgaatttcga aaaagccggt atgcttcaaa aagatatcga attagttact tattttatta
    16381 gacggatcca ggaacaaaag cagtttttaa gaacaccatc gctcaccttc agcatgccat
    16441 tagcagcaga tgagagtcag aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa
    16501 caatgattac ctctggtgac gtttctcctg acttctatta tgaaaagaaa gaagctcatt
    16561 tatctttgaa acgtcaattg tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt
    16621 accgcaaaaa attagaaaaa gaacagtttg aaattgaact gttaaacaaa aaagaagctg
    16681 aaaagcaact aaattagttg cttctcggct tcttttaggc atcttcaact tcaaatgtca
    16741 attatcctaa aatttattgg attaagtcat agatttccat cgctacgata tcgatattat
    16801 caaattcgta ttgctgatga gtatctgttt cagttaattc aaatgtatcg gtcgctttat
    16861 cataagttac tgtacacttc tctacgcctt ctttttcaaa acggcgaact tgcagttcat
    16921 tatctgctga ctcgaccata gcctccagtc gttttatgat tgcgactaat tgtgaatgtg
    16981 tcatcggagg tcctcctttt cttatttctg ttatattgta tcaaaagcat agcctacttg
    17041 cattgatttt ctctaaaaaa actcgttttt tatgattttt tttctggtat ttttccttca
    17101 ggccaccaaa gtttgatcaa agcttgggtt ccgtcattct caaaaccttt atctgccaac
    17161 tcttcatata gtttcaatgc ttgggtagtt gctggcaata caagatccat tttttctgct
    17221 tcttctaatg caatctttaa atctttgata aaatgtttga caaaaaaacc aggtgtgtaa
    17281 tcttctttta aaatacgagg agaataattg cttaaagacc agttagctgc acttccacct
    17341 gaaagcgtct caatcacttt ctttaaatca agtccgtttt tttgtgcata gacaagcatt
    17401 tccgtcattc ctgtcatcgt acctgcaatc atgatctgat tagccatttt tgtatgctga
    17461 cctttcccag cagaaccatg caatgtaaat gtcgtaccaa attctttgaa taaaggcagt
    17521 acttgatcat aaacggcttc ttctccaccg accatgatcg tcagtgtacc attctttgct
    17581 cctaagtcac caccggatac cggtgcatct aacgcatctg cgcctttttc ttttgctgtt
    17641 tttgcaattt tttctgccag gcttggtgta cttgtagtca aatcaactaa aatatgtcct
    17701 gagatatctg cagaaaaaat ccccgactgt ccatagtaga tttcttctac atctttgggg
    17761 taacctacca ttgtaaagag aatattactt tgttctgcta ccgcttttgg tgtatctgcc
    17821 cagatagctc cttctgttac taaaggatca gcttttgctt ttgtccgatt atagacagct
    17881 acttcgtgtc ctgcttctag tagatggcgt gcgacagcac ttcccatgac gcctgttccg
    17941 ataaaaccaa gtttcatttt ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta
    18001 gtatacgcta gaaacaagga ttttttcata aaaaatgttt gaattttatc ttttaaatga
    18061 atttaatttt attttgattc gttcaatcga gtcttcttct tcttttcttc gcatctcttc
    18121 ttcatgataa acaaccatgt cttgataaaa ttcgatcata gcttcgccga aatgtgtaga
    18181 tgaaatatca taaagtttag cttctaaaac ttttggatct ttaggaactt cactggaaat
    18241 ataatcaata agtgctggtg caaagtcttc gtcggtttca aaagttttcc ctaatgaagg
    18301 atgatcgaac aatcggttca aataagcatt tccttccacg acacaaggtg ttccggctgc
    18361 catcgcttct gtataagtaa gcccttgtgt ttctgaagta gaagcactga caaaataatc
    18421 tgccgcttta taataaagtg ctacatcatt gttgtccact tctcctgtga actgcacaaa
    18481 ttcttctaac tgcagttctg agatcaattc ctctaactct tctacataag gtccttttcc
    18541 tacgataact aatctggcgt taggataagc ggagatgacc tgaggaaacc cattaatcaa
    18601 ggcttggata tttttctcat aagaaatcct gcttaaagaa agaatcatca aatggttttc
    18661 ctgaagacct agtgactccc ttaatttgct gatatcctgc tgagtgatat cagaacgttt
    18721 gaatttttca atgtcgatac cagtaggaat cacgcgaagc ggtgctatta caccatagct
    18781 gcgtaatgta tcaataacac gttcacttgg acaaactaca cctgtcgtat gatttgcaaa
    18841 taatctagac aagtatttta catgggattg cctgatcact tttcctttac cgatataatg
    18901 aaggtagtct tcatacatcg tatggtaagt atggatcaga ggtattttca acttcttagc
    18961 taccatcttc ccaagtatcc ctgctccgaa ttctgtatgg gtatgaataa gatctagttc
    19021 tagttctttg gcaaccaaat aagcatacca catgcctcta acaacaactc ttcgatcctt
    19081 gaaagaaata aatggtacac ttggcattcg aatcacatct ttttccaact tatccgcatt
    19141 aggatcggtc gttgtaaaaa tatagacttc atgcccatgt ctttctaatt cttgttttaa
    19201 tgttttgatc gatgtagcaa ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc
    19261 gattttcatg tttttcgcct ccaaactagt ccttgacctg atttactttt tttaatttgc
    19321 tagagacttt ttcattcagt acactttcgt atacattttt aagttcttca ccgattttat
    19381 tgatacttcg ctcttttgct gtttgataac cggcttgtgt cgtactcggt agttcttgat
    19441 tggtaatctg ttggatcagt cggataaatt cttcgtttgt cttccccata tagcagttct
    19501 tgctatcttc taaccagcca ttatacactg gaatatctcg aacaagtacg ttttgtttgc
    19561 ttgctaaagc ttctaaaaca acgatccctt ctgtctcttc ataagaaggg aaaaagaata
    19621 gatctgcacc tgaatacgca ccttctatga tctctccacg aatgtaacca ggaaacagga
    19681 cattttcagg atgatcttct tttacaattt tccggatgtt ccgaggaatc gagtacatgg
    19741 gaacatgacc aaaccaaata aatgtgtatt cgggcatctt cttagcaatt tctacgaaat
    19801 caatgattcc tttacgttcg aaaaacaaac cgacacaaat gattactttt tgatccggag
    19861 acagtttgaa atattctctg aacttttgtt ctttcattgg atcagggtga tatttctcta
    19921 agtcgatccc attggaaata gcttgaatcg gcacacggat accatagctt tccaacaact
    19981 gttttgaata aggtgttggc gtgatcagat agtccgcttt ttggtacaat cctaccagat
    20041 atttttttac gataggagaa agctggttcg atccgataaa ggaatttcgg aaatcttcct
    20101 ctgtagaatg cgcatgatag acaacttttt ttccttctct tctggcttta ttgaccatat
    20161 tatgactatt gattccgtat gtattgatgt gcaggatgtc ataatcttct ttttcatcta
    20221 gtgtataaga gataccgact tccgtcagag cacgtttttg atgatcaagc gcacgcccga
    20281 ttccagattt tgcaagcatc ttttcacttt caaaatataa taataccttc accttacgtc
    20341 ccccttcact tatatagtat aacatagcct acaaactgtc actcaaactt aagccctatt
    20401 tttgtctatt cttactgaca aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc
    20461 tcttttacaa gccacaccag cttttgatac gttgtttatt ttacatattc atgaacaagt
    20521 tcaacgactt cttccatcat atcgcattct ttcaatgcac gatcagcaag ttcagccatt
    20581 ttcgctgtgt ccaagcgttt cattaaacta cgcgttttaa gaatagatgt cgcactcatt
    20641 gagaactcat ctagacccat acctactagc aatggaacag ctgtttgatc tccagccatt
    20701 tctccacaca tacctgccca ttttccttca gcgtgtgctg catcaattac atttttgatc
    20761 aaacgtaaaa ttgatgggtt atatggttgg tacaagtaag aaacgcgttc gttcatacgg
    20821 tctgctgcca ttgtgtattg aatcaaatcg ttcgtaccta cagagaagaa atctacttct
    20881 tttgcaaact tgtctgctaa gactgctgct gctggaatct cgatcatgat gccgacttgg
    20941 atcgtatcag atacttcctt gccttcactt atcaattttt gtttttcttc ttcaaagatc
    21001 gcttttgctg cgcggaattc tttgagtgta gctaccatag ggaacatgat acgtaaatta
    21061 ccatgaacag atgcacgaag caatgcacgc atttgtgtac ggaacatttc gtcgccttgt
    21121 tcagataaac tgatacgcaa tgcacgatat cctaagaatg gattcatttc atgaggtaat
    21181 tgcagatatg gtagttcttt atctccaccg atgtccattg tacgaacaac tacaggtttt
    21241 ccttccatac cttctaatac agctttgtaa gcttcatatt ggtcatcttc tgttgggaag
    21301 tctggagaat ccatatacag gaattcagta cggtacagac caactgcttc tccaccatta
    21361 ttatggacac caactagatc ttttggtgtt ccgatgtttg cagctaattc aaaatgcttg
    21421 ccgtcagctg tgacagtttc tgcatttttt aatttttccc attctgcttt ttgtgctgcg
    21481 taatctgcac ctgctttttc aaattcagct ttttgttcat ctgtaggatc gatgattaca
    21541 tcgccttcga tcccgttgac agccaaaata tcgccttctt tgactttagc agtaatttct
    21601 tttgtcccaa cgattgctgg aatttctaaa gaacgtgcca tgatagcaga atgtgaagta
    21661 cgtccaccaa tgtctgttac aaacgctttt acaaaattgc gatctaattg tgctgtatca
    21721 cttggagtca agtcatgtgc aacaacgacc acttcttcgt tgatcattga tggatttggt
    21781 aaagtaacgc ctaataagtg cgctaaaata cgtttagcaa cgtcacgaat atctgcagca
    21841 cgctcttgca tataagcatt atcttccatt gcttcgaaca taccgatata catatccgtt
    21901 acttctttta gcgctgattc agcatttacg ctgttgtctt taatattttg cttgatttga
    21961 cctaccattt ctggatctga aagaaccatc aaatgggcat caaatacttg cgcttctgct
    22021 tcacctaagc tttgcgctgc tttttcacga atttgctgca attcttcagt agatttagct
    22081 aatgcagcat ccaaacgagc ttcttcagcc gcagtatctt ctactgttgc cttactgaat
    22141 gttaaatccg gttgaactag caggtaagct tttgcaacgg ctacaccgtc actagcggca
    22201 atccctttta gcatttcaac cattattcag ataagccttc tttcttcatt gtttcaacga
    22261 ttgctgccat tgcatcagct tcgtcagcac cttctgcagt aatagtaaca tcagaacctt
    22321 gacctacacc taaagacata acgcccatga ttgatttcaa gtttactgat ttacctttat
    22381 actctaagtt tacatcagag ttaaatttgc ttgctgtttg tactaatagt gtagctggac
    22441 gagcgtggat cccagtttct gctactacgt gaaattcttt cttttccata ttaatcggtc
    22501 tcctttaagt gagtgttgtt tttatgtgag ggttttcttt tttagtaatt cagagaatcc
    22561 taacaaatgc caccctttca tctgtaaaga ttaacatttt ttttcgttag atacaacagt
    22621 tttcattcaa tttcagataa aagtattcaa attattccgg attctttttc atccttttct
    22681 tcacatttta taacaaacgt gtttatttcc tttttcttgt cgctttctct tctcctgacg
    22741 tttatcattc tttcctttga ccctctttgt tttcatatga aacgaattac ttgcttccct
    22801 ttttattttt agtataataa gagtcaaagg tcaggaatgg tcaatagaga tttcaccttt
    22861 tctacctcat aaatctctta aaaaactagg ggttattctt ttacctcatg aaaaacgaga
    22921 tgtaaaatat ggatgtaccc atttttgtga aaggacgtga atgattcatg ctttgtcaaa
    22981 actgcggcaa gaatgaggca acgattcact tgtatgccac tgtcaatggt cagcggacac
    23041 aactcgatta ctgtcagagc tgttatcaaa aaatcaaaaa tcaacaaaat ggaggtctga
    23101 ctaatatggc ccaaaatgat ccatttggat ttggcagttt ggacgatctt ttccgttcca
    23161 tgtctcgcca aatgcaacag caaggaaatt atgagcaaac accaccaaca caatttggcg
    23221 gaaacaacaa cttcaatggt ggtcagccac ctcaaggcaa tacagaaggt ttattgggag
    23281 aatacggaat caatatcaca gaacaagcac gacaaggtga tatcgatcct gtgatcggac
    23341 gcgatgacga aatcaaacga gtcattgaaa ttttaaatcg ccgaaccaag aacaatcctg
    23401 tcttgattgg agaaccaggt gttggtaaaa cagcagttgt tgaaggttta gcacaaaaaa
    23461 tcgtagatgg tgatgtaccg caaaaattaa tggacaaaga agtcattcgt ttagatgtcg
    23521 tatctttagt ccaaggtact ggtatacgag gacaatttga agaacgtatg caaaaattga
    23581 ttgaagagat ccgtcaagca gaaaatgtga tcctattcat agatgaagtt cacgaaatcg
    23641 tcggtgctgg ttctgctggt gatggaaata tggatgctgg taatatttta aaaccagctt
    23701 tagctcgagg agaattgcaa atggttggtg ctactacctt aaatgagtac cgcatcatcg
    23761 aaaaagacgc tgctttagaa cgtcgtatgc aacctgtacg ggtagatgaa ccaactgtcg
    23821 aagaaacgat cagtattttg aaaggtttgc aaaaacgtta tgaagactat catcatgtaa
    23881 aatatactga tgaggccatc gaagcagccg caactttatc caatcgttac gtccaagatc
    23941 gcttcttgcc ggataaagcc atcgatttgc tagatgaaac aggttctaag aaaaacttga
    24001 cgatccaaat cgttgatcct aaaacaatcg agaaaaaact gcaagaagca gaagaacaaa
    24061 aagttttagc ttctcgagaa gaagatttcg aaaaagcagc ttattatcgc gatcagatca
    24121 ataaacttca aaaaatgaaa gaacgtcagc tcactgaaga ggaaacacct gtgattaccg
    24181 aaaaagatat ggaaaaaatc gttgaacaac ggacaggtat tccagtgggt gaattaaaag
    24241 aaaaagaaca gacacaactg aaaaacttag cagatgactt gaaagctcat gtcatcggac
    24301 aagataacgc tgtagatcga gtagcgaaag cgattcgccg aaaccgtgtt ggattaaaca
    24361 aacagaatcg tccaatcggc tccttcttat ttgttggacc aactggtgta ggtaaaacag
    24421 agcttgcgaa acaacttgcc tacgaactat tcggttcaca agattcaatg attcgtttcg
    24481 atatgtctga atatatggaa aaacatagtg tctctaaatt gattggttct cctccaggtt
    24541 atgtcggata cgaagaagct ggtcaattaa cagaaaaagt acggagaaat ccttacagtc
    24601 ttgtcttgtt agatgaagta gaaaaagctc atccagatgt tttgcatatg ttcttgcaaa
    24661 tcttagatga tggtcgattg acagatgcac aaggaagaac ggtcagcttt aaagatacga
    24721 tcatcattat gacaagtaat gccggaactg gtaaagtaga agcaaacgtt ggttttggtg
    24781 cggcacgcga aggtgttact cgttctgtat tgaaccaatt aaacaactac tttacaccag
    24841 aatttctgaa ccgttttgac gggatcatcg aattcagtgc attatcaaaa gaaaacttga
    24901 tgacaatcgt cacactaatg ctagacgatg tcaaccaaat gctggcagca cagcaacttc
    24961 acatcgaagt tcctacgaat gtcaaagaaa aattagttga cttaggctat gatccatcta
    25021 tgggtgctcg tcctcttcga cgcacgatcc aagaacaaat cgaagatggt atcgctgaat
    25081 tttatcttga tcacccttct atccatgagt tgaaagcaaa actggataaa gatggaaaaa
    25141 tcatcgttac ttctaaacca gaacgtttgg ctaaagaatc agccgaagaa acggctgaat
    25201 aaatccagtt ttatagcgtg agacggtatt tctttgagaa atgccgtctc attctttttt
    25261 cagaaaagtc aaaatactgt tcattttata aaaaagaaat tataataaga gaaacaagca
    25321 attaaaaata cataaaagag gtgagcgcca tgaaattggt aaatgtaaca aacagccatt
    25381 cccgtcttgt attaaaccag cttgaaaaca ctgatgccca tctagtcaag gtctacacag
    25441 ccggaaatac cactattgtc tacactgaag cccctgaaca taatgaaatt ctgctgatca
    25501 atgacaaacg taaaatccag ccgaaagaaa tcgaagatgc taaaaattat ttcttagata
    25561 aaatcaaaga cgccgtttac cacgaggatg aaatcaaagt aatcgaattg gcaggccttg
    25621 ttgaaatctc gattccaaaa catgaattag gcgctacatt agccgtgtaa acaaattttg
    25681 aacataataa ttaaagagcg tgggacaaaa
//
LOCUS       DAFFQY010000033        25675 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFFQY010000033 DAFFQY010000000
VERSION     DAFFQY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.033"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            170..514
                     /locus_tag="LRB02_001303"
     CDS             170..514
                     /locus_tag="LRB02_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL4659516.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="LRB02_001304"
                     /pseudo
     CDS             515..934
                     /locus_tag="LRB02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="LRB02_001305"
     CDS             complement(1026..1376)
                     /locus_tag="LRB02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659517.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="LRB02_001306"
     CDS             complement(1342..2673)
                     /locus_tag="LRB02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL4659518.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="LRB02_001307"
     CDS             2929..3495
                     /locus_tag="LRB02_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4659519.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="LRB02_001308"
     tRNA            3640..3727
                     /locus_tag="LRB02_001308"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="LRB02_001309"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="LRB02_001309"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL4659520.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="LRB02_001310"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="LRB02_001310"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL4659521.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTILSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="LRB02_001311"
     CDS             complement(7450..7896)
                     /locus_tag="LRB02_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4659522.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="LRB02_001312"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="LRB02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4659523.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="LRB02_001313"
     CDS             8361..8879
                     /locus_tag="LRB02_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4659524.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="LRB02_001314"
     CDS             complement(8956..9912)
                     /locus_tag="LRB02_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4659525.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="LRB02_001315"
     CDS             10078..10884
                     /locus_tag="LRB02_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659526.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="LRB02_001316"
     CDS             10881..11870
                     /locus_tag="LRB02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL4659527.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="LRB02_001317"
     CDS             11867..12871
                     /locus_tag="LRB02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL4659528.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="LRB02_001318"
     CDS             13003..13545
                     /locus_tag="LRB02_001318"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL4659529.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="LRB02_001319"
     CDS             13565..14263
                     /locus_tag="LRB02_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL4659530.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="LRB02_001320"
     CDS             14284..14496
                     /locus_tag="LRB02_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL4659531.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="LRB02_001321"
     CDS             14496..15458
                     /locus_tag="LRB02_001321"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL4659532.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="LRB02_001322"
     CDS             15476..15871
                     /locus_tag="LRB02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL4659533.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="LRB02_001323"
     CDS             16034..16399
                     /locus_tag="LRB02_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL4659534.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="LRB02_001324"
     CDS             16396..18207
                     /locus_tag="LRB02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL4659535.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="LRB02_001325"
     CDS             complement(18269..18436)
                     /locus_tag="LRB02_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL4659536.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="LRB02_001326"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="LRB02_001326"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL4659537.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="LRB02_001327"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="LRB02_001327"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL4659538.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="LRB02_001328"
     CDS             21193..22173
                     /locus_tag="LRB02_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659539.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22364..23086
                     /gene="tsaB"
                     /locus_tag="LRB02_001329"
     CDS             22364..23086
                     /gene="tsaB"
                     /locus_tag="LRB02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL4659540.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="LRB02_001330"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="LRB02_001330"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL4659541.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="LRB02_001331"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="LRB02_001331"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL4659542.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="LRB02_001332"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="LRB02_001332"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL4659543.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8326 a         4398 c         5377 g         7574 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcgtatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cctcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt ttttctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tattt
//
LOCUS       DAFFQY010000034        25596 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFFQY010000034 DAFFQY010000000
VERSION     DAFFQY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.034"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LRB02_001333"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LRB02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL4659544.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LRB02_001334"
     CDS             808..1242
                     /locus_tag="LRB02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL4659545.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LRB02_001335"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LRB02_001335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL4659546.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LRB02_001336"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LRB02_001336"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL4659547.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="LRB02_001337"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="LRB02_001337"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL4659548.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="LRB02_001338"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="LRB02_001338"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL4659549.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="LRB02_001339"
     CDS             9513..10439
                     /locus_tag="LRB02_001339"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL4659550.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="LRB02_001340"
     CDS             10621..11514
                     /locus_tag="LRB02_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL4659551.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="LRB02_001341"
     CDS             11648..11773
                     /locus_tag="LRB02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659552.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="LRB02_001342"
     CDS             complement(11907..12539)
                     /locus_tag="LRB02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659553.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="LRB02_001343"
     CDS             complement(12687..13229)
                     /locus_tag="LRB02_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL4659554.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="LRB02_001344"
     CDS             13364..13753
                     /locus_tag="LRB02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659555.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="LRB02_001345"
     CDS             13757..14521
                     /locus_tag="LRB02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4659556.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="LRB02_001346"
     CDS             14650..15597
                     /locus_tag="LRB02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659557.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="LRB02_001347"
     CDS             15727..16467
                     /locus_tag="LRB02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659558.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="LRB02_001348"
     CDS             complement(16758..17426)
                     /locus_tag="LRB02_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4659559.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="LRB02_001349"
     CDS             17560..17847
                     /locus_tag="LRB02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659560.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18262..19800
                     /locus_tag="LRB02_001350"
     CDS             18262..19800
                     /locus_tag="LRB02_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL4659561.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19797..20762
                     /locus_tag="LRB02_001351"
     CDS             19797..20762
                     /locus_tag="LRB02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659562.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20749..22071
                     /locus_tag="LRB02_001352"
     CDS             20749..22071
                     /locus_tag="LRB02_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4659563.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22134..22238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22312..23055
                     /locus_tag="LRB02_001353"
     CDS             22312..23055
                     /locus_tag="LRB02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659564.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23070..24062
                     /locus_tag="LRB02_001354"
     CDS             23070..24062
                     /locus_tag="LRB02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4659565.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24074..24811
                     /locus_tag="LRB02_001355"
     CDS             24074..24811
                     /locus_tag="LRB02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659566.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24801..25522
                     /locus_tag="LRB02_001356"
                     /pseudo
     CDS             24801..25522
                     /locus_tag="LRB02_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
BASE COUNT         8864 a         4337 c         5324 g         7071 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt ttgttcgtgc
    18181 tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt atacttaaag
    18241 aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga aagaaaaggc
    18301 gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt gctgattgca
    18361 gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc
    18421 ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt aggtttactt
    18481 atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat ttacgctttg
    18541 cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc tattgaagaa
    18601 gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga gcttcccatc
    18661 gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat cggtacggct
    18721 actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt aggtattgac
    18781 cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct tgcaattata
    18841 ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat cattacttta
    18901 gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt gtttaaaaat
    18961 gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat ccaaatgtat
    19021 aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag
    19081 acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc tgagttcaca
    19141 ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca
    19201 aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga tatgacatta
    19261 ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc ttttgcgaac
    19321 gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat caaagcggga
    19381 ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga agcatatggc
    19441 ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc aatcaataat
    19501 ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga gtacgatcta
    19561 gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc gctgatgaaa
    19621 aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa
    19681 ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg
    19741 acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga agatcagtga
    19801 ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg
    19861 acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct tccggtagtg
    19921 gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca ggagacatct
    19981 acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg aatatgggat
    20041 atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat attgaagtca
    20101 tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc
    20161 aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag ttgtctggag
    20221 gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat gtgattttga
    20281 tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa gaattggttt
    20341 tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat atggaagaag
    20401 caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc caatgtgaca
    20461 caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc tttgatgaac
    20521 cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat ttcttaaatg
    20581 aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa gaagtatatt
    20641 cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt attgggtcgc
    20701 tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat gaaaacatat
    20761 gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa
    20821 gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt
    20881 ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa
    20941 ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact tcaaaaattc
    21001 aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc agaagctgaa
    21061 atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga agtcaacgaa
    21121 gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac gatcttacca
    21181 gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc tgtactgcct
    21241 aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac gattgccaat
    21301 gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa agaatttgag
    21361 gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca gtttgctttt
    21421 ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt cggaaaagag
    21481 acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa tacagaatct
    21541 ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt caatgattat
    21601 ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag gaaaacccca
    21661 aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat aacggatgca
    21721 acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag tcctaaactt
    21781 gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt agagatggat
    21841 cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc
    21901 tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc agacgaattg
    21961 atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt ggatagctat
    22021 atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata atcagcatga
    22081 taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat gaatacctgt
    22141 ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca ttgaacctgt
    22201 cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta ctatgtcgtg
    22261 cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa aatgaagaaa
    22321 aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat tgttgtcata
    22381 gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc tgcattagcg
    22441 gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc agtgcttggt
    22501 ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta ttggaagttt
    22561 ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat catggtgtta
    22621 gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc
    22681 ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa agtatcaaaa
    22741 gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta tcgaatatat
    22801 aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc tacttattgt
    22861 gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg gtattacatg
    22921 atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt
    22981 ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta ttatcgtatg
    23041 ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta ttaccgctac
    23101 tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg
    23161 atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat actggcatct
    23221 acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa atcatcgaac
    23281 caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa tttggtgtaa
    23341 gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca gtcaaagcaa
    23401 ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac agtaacatcc
    23461 attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag
    23521 ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg acaatggtag
    23581 gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa tggtactttg
    23641 aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat atcccattga
    23701 aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat gatcaactga
    23761 tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg
    23821 aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca aaagaaaatg
    23881 atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg gatgatcctg
    23941 agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa
    24001 acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag tttataaaat
    24061 aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg aaaacctgaa
    24121 aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga ttgtcgcgcc
    24181 aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc ctatgacttc
    24241 tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat ttagttatat
    24301 gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta cgttatatca
    24361 aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg atatctttgg
    24421 gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga agcagcgaac
    24481 ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg aaccatttgg
    24541 agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc tgcctaaaaa
    24601 agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt atttatcaga
    24661 caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc aagtgcctga
    24721 aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa cgatttatca
    24781 gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat gggagtcaga
    24841 gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt gattcgccag
    24901 aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta agttacggta
    24961 tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag
    25021 caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg atgactacac
    25081 aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact
    25141 gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta
    25201 tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta
    25261 tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt
    25321 cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg
    25381 ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc
    25441 tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg
    25501 tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt
    25561 ggctttcttt gtataaaagt aaactgtttg attaat
//
LOCUS       DAFFQY010000035        25374 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFFQY010000035 DAFFQY010000000
VERSION     DAFFQY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.035"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="LRB02_001357"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="LRB02_001357"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL4659567.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="LRB02_001358"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="LRB02_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL4659568.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="LRB02_001359"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="LRB02_001359"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL4659569.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="LRB02_001360"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="LRB02_001360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL4659570.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="LRB02_001361"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="LRB02_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL4659571.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="LRB02_001362"
     CDS             5264..5842
                     /locus_tag="LRB02_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4659572.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="LRB02_001363"
     CDS             5894..6376
                     /locus_tag="LRB02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL4659573.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="LRB02_001364"
     CDS             6493..6867
                     /locus_tag="LRB02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659574.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="LRB02_001365"
     CDS             complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="LRB02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL4659575.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9166..9756
                     /locus_tag="LRB02_001366"
     CDS             9166..9756
                     /locus_tag="LRB02_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL4659576.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9884..11233)
                     /locus_tag="LRB02_001367"
     CDS             complement(9884..11233)
                     /locus_tag="LRB02_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659577.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11401..12141)
                     /locus_tag="LRB02_001368"
     CDS             complement(11401..12141)
                     /locus_tag="LRB02_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4659578.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12154..13746)
                     /locus_tag="LRB02_001369"
     CDS             complement(12154..13746)
                     /locus_tag="LRB02_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL4659579.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14314..14610)
                     /locus_tag="LRB02_001370"
     CDS             complement(14314..14610)
                     /locus_tag="LRB02_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659580.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14786..15511
                     /locus_tag="LRB02_001371"
     CDS             14786..15511
                     /locus_tag="LRB02_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL4659581.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15504..16346
                     /locus_tag="LRB02_001372"
     CDS             15504..16346
                     /locus_tag="LRB02_001372"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL4659582.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16349..16870
                     /locus_tag="LRB02_001373"
     CDS             16349..16870
                     /locus_tag="LRB02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL4659583.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17123..17686)
                     /locus_tag="LRB02_001374"
     CDS             complement(17123..17686)
                     /locus_tag="LRB02_001374"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL4659584.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17937..18206
                     /gene="rpsO"
                     /locus_tag="LRB02_001375"
     CDS             17937..18206
                     /gene="rpsO"
                     /locus_tag="LRB02_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL4659585.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18394..20520
                     /gene="pnp"
                     /locus_tag="LRB02_001376"
     CDS             18394..20520
                     /gene="pnp"
                     /locus_tag="LRB02_001376"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL4659586.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21030..21296
                     /locus_tag="LRB02_001377"
     CDS             21030..21296
                     /locus_tag="LRB02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4659587.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21473..23431
                     /locus_tag="LRB02_001378"
     CDS             21473..23431
                     /locus_tag="LRB02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL4659588.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23785..25086
                     /locus_tag="LRB02_001379"
     CDS             23785..25086
                     /locus_tag="LRB02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL4659589.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4807 c         5202 g         7192 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaca aaatatttct
     7441 taatacttga aactaagcgg ctttttcacg acctctcaat aaacggtgtt caaaagtcag
     7501 tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca aaaaatattt
     7561 cttaatactc gaaactgagc ggctttttca cgacctctca ataaacagtg ttcaagagac
     7621 agcctatcgg caataaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
     7681 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
     7741 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatat aaaagatttt
     7801 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
     7861 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
     7921 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaacaggca
     7981 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
     8041 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
     8101 aagctgtaat caaaccaata ccggtcttca agcaagctaa tataacgata aatgctaata
     8161 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
     8221 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
     8281 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
     8341 caggttttgt tacacccatg ttttggattg ttgtgacaat gatgatccca aatgctaaag
     8401 cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc tgtgaaaaaa gcatgctctt
     8461 gataatttgg ctgaataggc gcttgattta cagaacctag tggattcaaa aaagcaagga
     8521 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
     8581 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
     8641 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
     8701 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
     8761 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
     8821 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
     8881 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
     8941 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
     9001 atgataattt ttttgtcata cataactcct ttacggtttt tttaaaagta acaaaatttc
     9061 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgacggtta tttaataacg
     9121 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
     9181 ttagaagcat ttactgatgg agtggtagcc attgttttga gagttcttgt attagagatc
     9241 aagatcccta atacatctag ctttcattct ttatgggtca tccgaaatac attattagct
     9301 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
     9361 atggcagaac gaattaacta ccgagtggtc tggtcgaata tctttttgct cttttggctg
     9421 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
     9481 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
     9541 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
     9601 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
     9661 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
     9721 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctggga cagaagcttt
     9781 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
     9841 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
     9901 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
     9961 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    10021 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    10081 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    10141 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    10201 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    10261 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    10321 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    10381 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    10441 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    10501 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    10561 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    10621 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    10681 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    10741 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    10801 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    10861 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    10921 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    10981 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    11041 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    11101 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    11161 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    11221 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaaa
    11281 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    11341 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctattgatc
    11401 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    11461 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    11521 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    11581 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    11641 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    11701 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    11761 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatctcgtt tcaggacagt
    11821 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    11881 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    11941 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    12001 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    12061 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgtttgat
    12121 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    12181 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    12241 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    12301 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    12361 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    12421 gatattccgt aaaacttgtg gaataacaac ttttctcatc gtttggccat gggtcatacc
    12481 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    12541 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    12601 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    12661 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    12721 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    12781 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcacctcg
    12841 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    12901 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    12961 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    13021 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaagccat ctgcaccgct
    13081 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    13141 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    13201 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    13261 taactgagca gtaatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    13321 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    13381 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    13441 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    13501 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    13561 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    13621 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    13681 tcccacactg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    13741 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    13801 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    13861 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    13921 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    13981 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    14041 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    14101 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    14161 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct attcattgca
    14221 aacgatagta taattataca caaaaatgta tgtaatacaa taaaaaaaga actagaacag
    14281 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    14341 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    14401 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    14461 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    14521 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    14581 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    14641 ttgtaacacg ttttcattct tttgtcagtg aacattgtga taaattgttc aaatgcccat
    14701 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    14761 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    14821 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    14881 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    14941 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    15001 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atggcacatc tagcgaaaga
    15061 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    15121 gcaactcgtc aattttcatg gaagtttgta tcaatgtcat tgtcgaaaat gtcaacaaac
    15181 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcatgcggag gacaaatacg
    15241 cccagacatc gtgttatatg gtgaagggtt tcaagatgga gtcatggagc aggcggctta
    15301 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    15361 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    15421 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    15481 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    15541 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    15601 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    15661 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    15721 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    15781 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    15841 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    15901 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    15961 gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa atcttttccc gacaatgtag
    16021 tgattgcagg aaatcctgct cgtatcatca aggcagtaga ggaagaacta acagaagagt
    16081 ctcttgaaac attacgtcat aagatcgata tgatcgatcg agagttggtt gctttattag
    16141 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaagcag
    16201 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctg ctgaaaaata
    16261 agggatataa ggaaacaata acagatacgt atgttgattt gatgaaacac tcgcgcgaat
    16321 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    16381 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    16441 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    16501 tatcgtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    16561 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    16621 tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    16681 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    16741 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    16801 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    16861 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    16921 taaacaccca tatggaagaa ataagaaaaa ataagaaaag atgcgtatcc aatacacaga
    16981 attttttgga attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa
    17041 aaaagggaag tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt
    17101 atcgaaggaa cctgccaatt ctctactcga tcaccaatac gccttctttt agtgcaaatg
    17161 gattttgatc gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt
    17221 gaacgacgat cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat
    17281 aataagacac agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag
    17341 ataaacagcc ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag
    17401 gattatacat aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt
    17461 gaacagcaat gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc
    17521 gtaagtgtag ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct
    17581 cttttcctaa agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc
    17641 gtaaggtcgg atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc
    17701 tttcttaaaa gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa
    17761 aagtagtagt aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca
    17821 acccggtttc aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa
    17881 gcgaatcacc aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg
    17941 caatttcaaa agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata
    18001 ctggttctcc agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac
    18061 atgcacgcgt gcacaaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc
    18121 accgccgtaa cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga
    18181 tcaaacgttt aggcttgcgt cgttaatttt agtcaaaaag agtgggattc acatgaatct
    18241 cacttttttt actcgtatat caattaagta aaattccgtg atcaagctag atggactcct
    18301 actaaccaaa tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc
    18361 gtcttgcttt cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg
    18421 acatggggcg gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga
    18481 gccgttttag tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa
    18541 gcaaaagata cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca
    18601 gggaaaatcc ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg
    18661 actgcccgtt tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa
    18721 gtccaagtaa cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca
    18781 atgctaggtt cttctttagc actatcaatc tcagatatcc catttgacgg cccaatcgca
    18841 ggcgtagaag ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca
    18901 gaacaaacag atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa
    18961 agcggcgcga aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac
    19021 gctatcaaag agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa
    19081 atggaagtga ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct
    19141 tacgctacaa tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc
    19201 gagcaggtga agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat
    19261 gaagatcact tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta
    19321 cgtgaattga tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt
    19381 ccattgtcat cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga
    19441 ggacaaacac aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt
    19501 gatggtttag gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt
    19561 tctgttggtt ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca
    19621 ttaggtgaac gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc
    19681 cgtttagtag cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct
    19741 ggcacattag cattgatgga tgcaggtgta ccaatcaaag cgccagtcgc cggtattgcg
    19801 atgggacttg tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa
    19861 gatcatcttg gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta
    19921 cagatggata tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa
    19981 gcaaaacaag cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt
    20041 gaagaattaa gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc
    20101 aaagatgtta tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg
    20161 aaaatcgata tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc
    20221 aaaaaagcta tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat
    20281 ctagggaaag ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgat caaaggaaaa
    20341 gatggacttg tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt
    20401 gttaaactag gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta
    20461 aatctgtcaa gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaataa
    20521 tttaattcat aggaataatg tacttgtgat cgtgaaggtg acagggggac aataattatt
    20581 cgaaaaaagg ggactgtgac aaaagccgtg tcacagtcct tttttttcga ataaacggtg
    20641 ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacatta ggagctgttt
    20701 ttacttggct gttcttatta cttgatgcag aacagctttt ttacaacctc tccaataaac
    20761 gatgttcaat cagctgtccc tcgatattag ctcaaaaaac ggcaaaatat gaggaactat
    20821 ttttccgttt gtttctctaa tactcaggtc aaaccgctga tttcacaacc tctgattcac
    20881 ggtgttccag aagcagcttc tacggaaata agctgaacac ttttgttaca acttcttttt
    20941 ttctggagtt tcttggagca tatttttgga gtccttctga ataaagcgta aaataaaagt
    21001 gagaaatatg gataaaggag ggaaaggaga tgctgaattt tttatggtcg ttatttatcg
    21061 gcggtatatt aggtttgata gctggcgcaa ttttaggaaa ggaagtcccg ggaggcgtat
    21121 ctggaaatat cattatcggt tttttaggta gttggctcgg cgaattttta ctaggaccac
    21181 tgggaccgga actcggcggt ttttatttga taccagcatt attgggtgca attttttgcc
    21241 ttgctcttta ttcatttgtg gcaagacgta tcagagaacg tccaaagaaa gaataatttt
    21301 attcaaataa gttgacactt cagtgtgtta cttggataat tagaagcaat ttaggaaagc
    21361 gggtgtcatg gataagaata tcatctatca ttcaagctta cctaaaggat atccatataa
    21421 caagaaatga gggaaaaacg tggcaacaac gcacaatacc aaaaagatat caatggctgg
    21481 cctgcttgta gcgatgggag tcgtctacgg agacatcggg acaagtccgc tttacgttat
    21541 gaaatcgatt gttgaagata atggcggatt gtcaggaatc aatcctgatt ttgttatcgg
    21601 atcagtctca ttgatttttt ggacattgac tctactgaca acaatcaaat atgtcgtgat
    21661 tgcattgaat gcagataacc atggtgaagg tggaattttc tcactctaca cacttgtccg
    21721 aaaaggtggg aaatatttga tcattcctgc aatgatcgga ggagctgcct tactagcaga
    21781 tggggtattg acccctgctg tgacggtaac gactgcgatt gaaggcttgc gaggaattcc
    21841 gcagtttttt gaccggttcg gtaataacca gaacgtgatc gtcattatca ctttagttat
    21901 tatttttgtt ttattcatgg tccaacgctt tggtacagat attgtcggaa aagcattcgg
    21961 accaatcatg ttcgcttggt ttactttttt aggcgtaatg ggattaatga acttcagtca
    22021 agactggaca gttatccgtg cactgaatcc ttattatgct atccagctgc tttttagtgc
    22081 agataacaaa ttaggcttgt tcattcttgg aaatattttc ctggctacga caggtgcaga
    22141 ggcgctttat tccgatcttg gacatgtcgg caaacgaaat attcgtctga gctggcctta
    22201 cgtcaaaatt tgtttggtta tcaattattt tgggcaggct gcgtggttgt tgaatgttta
    22261 tcaaaaccct gaagcacagc aaatcaagaa cctgaatcca ttcttccaaa tgatgcctca
    22321 aagttggatc gtgatcggcg ttgcttttgc aacagtcgct gcagtcattg cttcgcaagc
    22381 attgattaca ggttcgttta cgctagtttc agaagcaatc aagttgaaac tgctgcctcg
    22441 gttaaagatc atgtatcctg ggaacagtat cgggcaaatg tatatcccag cagtcaatct
    22501 gattttatgg atcgcctgtt ctctagtggt tgtcacattc cgaacgtctc atcatatgga
    22561 agctgcttac ggtctatcga tcactgtgac gatgttgatg acaacagcac tgctttactt
    22621 ttatcttatc caaaatgggc attctcgttg gcttgcatat ttagttacct ttttcttcgg
    22681 tgcgatagaa gtcgttttct ttatctcaag tgttgtcaaa ttcttccgag gcggttttgt
    22741 agccgttctg attggtttga tcatcttagt cgtgatgttt atttgggaac aagggaacat
    22801 tattcgcgaa tcgtcaaccg aggatgtgtc tttgagagat tatatcccgc agcttagaga
    22861 attacgtgat gatacttctt taccaatcta tcagacaaat gtggtcttct tggttcctga
    22921 tatggctgaa gataaagttg gccgacagtt catctattcg attttagata aacggcctaa
    22981 aagagcacga gtctattggt ttgttcatgt ggaagtgacg gatgagccat atacgaaaga
    23041 atatcaagta gatatgatgg acactgattt tattgtccaa gtgaatctgt tcttaggctt
    23101 cagggtccaa caggaaatca acgtatatgt tcgccaagtc atccatgatt tgatgaaaca
    23161 aggccgtttg ccaaaacagc cacaaagata ctctctgaca ccagggcgag aagttggcga
    23221 tttccaattt gtcctgattg atgaagtagt ttccaatgtg acaactctag gaaaatggca
    23281 acgtcaaatc atgcaggcaa aactggcaat caaaaaaatc gctacctcgc cagaaacatg
    23341 gtttgggcta gagtacagcg aagtaaaaca tgaaactgtt ccgcttatca ttggaaatac
    23401 tcggaaaaca tggttgaaag agcggttgtg aaagtagcgc tttgacctga actaatgtcg
    23461 aatgtcagct acttgaacac cgtttatcag aggtcatgaa aaagctgtac tgcaacaaac
    23521 agtaagaacg agcagataaa aacagcttct catgtttttc atctgtttgg gcttaatgcc
    23581 gcagatgcaa gcttttgaat accgttttta tcagaggtca tgaaaaagct gtttagcaac
    23641 aagtaacgca atgattttga agtgataagg agatgtggct gaaaaatgcc gcatctcctt
    23701 ttttattcct aaatatgaat acgcattatt ttcaatcttt ttgtagaaaa agggtacaat
    23761 agaaaaagag aaaggcggga gaatatggtt atcaatttca ttattttatt tttgatgatt
    23821 gcaattactt cattttttgt agcaagtgaa tttgctttag tgaaaatcag acgatcaaga
    23881 ttggaacaac tagaaaagga aaatgtaaag aacgccaagc tagctcttca tgttacacac
    23941 catttagata actatttatc agctagtcaa ttaggaatta cgctaaccgg tttgatcatc
    24001 gggtgggtcg gtgaaggatc tgtcgcggct ttactagaac cagtgatcgg taaactacct
    24061 tttagttcgg ctattagcag tacaatctct gtggcgctgg gatttatttt agttacgtat
    24121 atcgatgtgg ttattggcga actgcttcct aagagctata gtatcgtcaa tacggagaag
    24181 gtcgtcttgt tcgtagttaa accacttcat tatttttata aagtaatgtt tccttttatt
    24241 tgggtattga atcattcagc tgcagggcta gggaagctat taggtgtgcg tctcgtatct
    24301 gaaggagaag agacattgtc tcaagaagag ctgacattag tcgcattgaa ttcttatgaa
    24361 aaaggagagt tgaccaaaga agaatatcat tatttggaaa acgtgtttga atttgatgat
    24421 accttggcaa aagatataca agtagaccgt acgtccatgc aagtattcga agcttcagaa
    24481 accgtcaaag aagcgataaa agcttcgatt gaacaaggac atactcgcta tccggttatc
    24541 ttagaatcaa aagacaatgt tttaggttat gtcaccttgc cagatttgat caagcaatct
    24601 tataaagatg accagctgac agtagaacag ttgattgaag agccgatcgt aacaactgaa
    24661 acgatcccta taaaaaaatt attgacgatc atgcgaaaaa agggaaagca tattgctatc
    24721 ttaaaagatg aatacggagg gacaagcggc ttagtgacga ttgaagatat tttagaagaa
    24781 atcgtcgggg aaatccgaga tgagacagat ttggacgaag cattgatagc ggaacagtca
    24841 gatggctctt atattatctc cggcaaacta acattagatg atttccagcg ttattttcat
    24901 gtagagattc cagagtttga agaaacgaat tttactacac ttgcgggctt tgcttctagc
    24961 cgatataaag aaataaaagc aggaacaatc attgagattg catcattccg tttcacggta
    25021 ttagaatacc agcatgcaca tatcgattat ttcaaagtag agtccacgga aagaaaaaca
    25081 gaataaaaga aaaaaggctg tgacattttt tgtcacagcc tttttcctac gattagtcaa
    25141 gtaaaaggtg caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat
    25201 aaagccccgt tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt
    25261 tagttaatgg tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat
    25321 agcgtgcata catttcagaa gcttgttcat acaagcaact gtggcaacct tatc
//
LOCUS       DAFFQY010000036        25110 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFFQY010000036 DAFFQY010000000
VERSION     DAFFQY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.036"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            365..1276
                     /locus_tag="LRB02_001380"
     CDS             365..1276
                     /locus_tag="LRB02_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL4659590.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1493..1930
                     /locus_tag="LRB02_001381"
     CDS             1493..1930
                     /locus_tag="LRB02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659591.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2025..2909
                     /locus_tag="LRB02_001382"
     CDS             2025..2909
                     /locus_tag="LRB02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL4659592.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3133..6300
                     /locus_tag="LRB02_001383"
     CDS             3133..6300
                     /locus_tag="LRB02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659593.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6320..6865
                     /locus_tag="LRB02_001384"
     CDS             6320..6865
                     /locus_tag="LRB02_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL4659594.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6893..7348
                     /locus_tag="LRB02_001385"
     CDS             6893..7348
                     /locus_tag="LRB02_001385"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL4659595.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7345..8103
                     /locus_tag="LRB02_001386"
     CDS             7345..8103
                     /locus_tag="LRB02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL4659596.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8189..8929)
                     /locus_tag="LRB02_001387"
     CDS             complement(8189..8929)
                     /locus_tag="LRB02_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659597.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8990..10243
                     /locus_tag="LRB02_001388"
     CDS             8990..10243
                     /locus_tag="LRB02_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4659598.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10565..10792
                     /locus_tag="LRB02_001389"
     CDS             10565..10792
                     /locus_tag="LRB02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL4659599.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10915..11241
                     /locus_tag="LRB02_001390"
     CDS             10915..11241
                     /locus_tag="LRB02_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL4659600.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11309..12022
                     /locus_tag="LRB02_001391"
     CDS             11309..12022
                     /locus_tag="LRB02_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL4659601.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12332..13182
                     /locus_tag="LRB02_001392"
                     /pseudo
     CDS             12332..13182
                     /locus_tag="LRB02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            13303..15168
                     /locus_tag="LRB02_001393"
     CDS             13303..15168
                     /locus_tag="LRB02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4659602.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15216..16625
                     /locus_tag="LRB02_001394"
     CDS             15216..16625
                     /locus_tag="LRB02_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL4659603.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16733..17434
                     /locus_tag="LRB02_001395"
     CDS             16733..17434
                     /locus_tag="LRB02_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4659604.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQICIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17593..18072
                     /locus_tag="LRB02_001396"
                     /pseudo
     CDS             17593..18072
                     /locus_tag="LRB02_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18920..19495
                     /locus_tag="LRB02_001397"
     CDS             18920..19495
                     /locus_tag="LRB02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL4659605.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19495..19935
                     /locus_tag="LRB02_001398"
     CDS             19495..19935
                     /locus_tag="LRB02_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL4659606.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19951..20652
                     /locus_tag="LRB02_001399"
     CDS             19951..20652
                     /locus_tag="LRB02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4659607.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21140..21565)
                     /locus_tag="LRB02_001400"
     CDS             complement(21140..21565)
                     /locus_tag="LRB02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659608.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21939..22910
                     /locus_tag="LRB02_001401"
     CDS             21939..22910
                     /locus_tag="LRB02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659609.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22942..24339
                     /locus_tag="LRB02_001402"
     CDS             22942..24339
                     /locus_tag="LRB02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4659610.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24498..25106
                     /locus_tag="LRB02_001403"
     CDS             24498..25106
                     /locus_tag="LRB02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659611.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8572 a         4132 c         5294 g         7112 t
ORIGIN      
        1 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
       61 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
      121 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
      181 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
      241 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
      301 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
      361 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
      421 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
      481 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
      541 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
      601 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
      661 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
      721 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
      781 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
      841 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
      901 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
      961 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     1021 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     1081 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     1141 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     1201 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     1261 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     1321 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     1381 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     1441 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     1501 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     1561 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     1621 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     1681 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     1741 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     1801 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     1861 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     1921 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     1981 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     2041 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     2101 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     2161 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     2221 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     2281 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     2341 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     2401 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     2461 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     2521 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     2581 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     2641 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     2701 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     2761 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     2821 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     2881 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     2941 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     3001 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     3061 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     3121 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     3181 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     3241 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     3301 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     3361 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     3421 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     3481 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     3541 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     3601 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     3661 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     3721 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     3781 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     3841 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     3901 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
     3961 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
     4021 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
     4081 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
     4141 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
     4201 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
     4261 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
     4321 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
     4381 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
     4441 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
     4501 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
     4561 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
     4621 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
     4681 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
     4741 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
     4801 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
     4861 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
     4921 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
     4981 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
     5041 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
     5101 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
     5161 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
     5221 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
     5281 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
     5341 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
     5401 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
     5461 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
     5521 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
     5581 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
     5641 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
     5701 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
     5761 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
     5821 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
     5881 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
     5941 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
     6001 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
     6061 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
     6121 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
     6181 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
     6241 atagctggta attactcagc agagataaca tggacattag cagatgcgcc gacagaataa
     6301 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
     6361 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
     6421 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
     6481 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
     6541 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
     6601 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
     6661 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
     6721 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
     6781 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
     6841 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
     6901 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
     6961 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
     7021 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
     7081 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
     7141 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
     7201 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
     7261 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
     7321 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
     7381 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
     7441 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
     7501 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
     7561 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
     7621 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
     7681 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
     7741 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
     7801 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
     7861 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
     7921 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
     7981 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
     8041 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
     8101 taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct
     8161 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
     8221 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
     8281 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
     8341 attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag
     8401 tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga
     8461 tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa
     8521 taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt
     8581 gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc
     8641 gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg
     8701 catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga
     8761 tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt
     8821 gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga
     8881 tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc
     8941 tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac
     9001 agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc
     9061 gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt
     9121 acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt
     9181 ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat
     9241 cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct
     9301 tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag
     9361 tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg aacactattt
     9421 tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca
     9481 cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc
     9541 tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc
     9601 gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga
     9661 ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt
     9721 ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca
     9781 acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa
     9841 agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc
     9901 gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca
     9961 tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg
    10021 ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag
    10081 aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga
    10141 tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt
    10201 agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata
    10261 agtttgagga aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag
    10321 aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt
    10381 gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga
    10441 agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag
    10501 ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa
    10561 aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc
    10621 ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag
    10681 agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc
    10741 aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat
    10801 gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat
    10861 gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat
    10921 agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa
    10981 aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg
    11041 aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat
    11101 gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt
    11161 gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga
    11221 aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa
    11281 cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct
    11341 agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca
    11401 gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa
    11461 agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc
    11521 aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc
    11581 tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac
    11641 gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta
    11701 tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt
    11761 agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt
    11821 ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga
    11881 aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg
    11941 agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa
    12001 tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc
    12061 aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg
    12121 tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat
    12181 atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat
    12241 tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt
    12301 ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa
    12361 tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc
    12421 ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgtttt
    12481 aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga
    12541 gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc
    12601 gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga
    12661 aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aattttatcc gaaagaatat
    12721 caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag
    12781 gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg
    12841 caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc
    12901 ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag
    12961 ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac
    13021 gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat
    13081 cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat
    13141 attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc
    13201 aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct
    13261 gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt
    13321 ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt
    13381 gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa
    13441 gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc
    13501 agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag
    13561 gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata
    13621 tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg
    13681 gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgaa
    13741 gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc
    13801 aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca
    13861 gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca
    13921 tctggttata cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta
    13981 gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc
    14041 gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc
    14101 ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt
    14161 ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcatcg gggattcgta
    14221 ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt
    14281 ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg
    14341 aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca
    14401 actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga
    14461 ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc
    14521 agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca
    14581 gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta
    14641 ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa
    14701 ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg
    14761 agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat
    14821 ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact
    14881 ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg
    14941 gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc
    15001 aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc
    15061 gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac
    15121 caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag
    15181 gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat
    15241 tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag
    15301 gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa
    15361 aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg
    15421 ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt
    15481 ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga
    15541 ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc
    15601 ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg
    15661 aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt
    15721 tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca
    15781 atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta
    15841 gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta
    15901 tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc
    15961 aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat
    16021 acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa
    16081 aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt
    16141 ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag
    16201 gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc
    16261 tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa
    16321 acggtcttgg agccattgat catatggaag aggatggcac gattattgat gattatcgga
    16381 tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt
    16441 tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga
    16501 gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc
    16561 gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact
    16621 tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt
    16681 tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag
    16741 gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa
    16801 atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag
    16861 caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa
    16921 aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc
    16981 aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt
    17041 gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt
    17101 ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct
    17161 tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt
    17221 gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt
    17281 aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atctgcatca aacggatacg
    17341 gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga
    17401 gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct
    17461 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg
    17521 ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg
    17581 ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg
    17641 atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat
    17701 atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag
    17761 atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg
    17821 ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt
    17881 ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca
    17941 ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat
    18001 ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg
    18061 acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga
    18121 gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta
    18181 ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa
    18241 tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa
    18301 aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat
    18361 tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc
    18421 aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat
    18481 ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa
    18541 aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta
    18601 tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt
    18661 gttgtttaaa ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag
    18721 taagtcattt catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct
    18781 gaaaaatgat ttttttctaa atactatata tagtggcgtc tatcctatat accactaaaa
    18841 aactacaaaa tctagtcttt tattttaaaa aaacacgtgt agaatgaaga tagacttttg
    18901 agttagggag tgttttttta tgaaacttac tgttttttgc ggttcccgtt ttggtaataa
    18961 agaaagttac aaatttattg cgcaaacgtt aggaaaatac atggcacaag aagaaatcga
    19021 actggtttac ggtggttcat actccgggat tatgggagtg attagtaaaa cagtactgga
    19081 aaataacgga aaagtcacag gtatctatcc gaatgggctt tttgaaactg aattgcctgg
    19141 aaaagatgtt actacattta ttcctacaga aacgatcgat gaaagaaagg tattattatt
    19201 tgaaaaggga gatgctgtcc tcgttttccc tggaggtcta ggtacattgg aggaattctc
    19261 acaacttctt tcttggatag caatcggtct gacgcccgac aagccaatcg gaatattgaa
    19321 tattggcggt tattacagcg gcttgcaaca attattagaa acttttgcaa aagaggattt
    19381 tatggataaa aaatggttgg atcaagtaat cttttccaac aatccattgg aactggtcaa
    19441 tgaattacga gcagtatcta ttaaaaatca gttattattg aaggaggcaa aataatgagt
    19501 caaatatatt tagcaggacc atttttttca gaggagcaaa tcgatcgagt aagtcgtatc
    19561 gaaaaagcgt tggaggaaaa caaaacagtc acaagtttct actctccacg tcatcaccaa
    19621 gaaagcaatt atgaactatt cagcgctgga tgggcgcagg aagtatacga aaaagatatg
    19681 gaagaactga caaatgcaga atttgttgtt gctatccttg actttgagca tcaaacaatc
    19741 gatccaggaa cggcttacga attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt
    19801 gtacaagaag agacggtacc tacgaacttg atgattacac aaagtctcca tacgtattta
    19861 aaatcagacc aagctgtacg tgaatatgat tttgaaacat tgcctgttga aacttacgta
    19921 ggcgagtact tatagaagag gaagggttgt atgtcagtga ttttgatttc aggaacgatt
    19981 ggtgcaggta agagcagttt gacggatatg cttgcaaaag agattgattc taaaccattt
    20041 tatgaaaatg tcgaagataa cgaagtattg ccgctctttt attcaaatcc tgagcagtac
    20101 gcttttttat tgcaaatctt ctttttgaac aagcgatttt tagcaatgaa aaatgcttta
    20161 gtgaacgatg acaatgtgct agaccgatcc atctatgaag atagcctgtt gttccatcta
    20221 aacgctgatt taggtcgggt aacggatatc gaagtacagc agtacgatag cttattagat
    20281 acgatgctaa acgagttaga tgatgttgct ccaaaaaaac ggcctgattt gatggtccat
    20341 atcaaagttt ctttggacac gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa
    20401 cagctagaaa gcgatgagac gttatatacc tattatgaaa cgttgaacac ccgctataat
    20461 caatggtatg aagattttga tgtatgtcct aaaatacaag tggatgggga taaattcgat
    20521 tttgtagaaa atccagaaga tgcaaaaaag gtgatccgtc agatccaaga aaaattagct
    20581 gaattggaag gaaagcctgc tggaaaatat tttacggata accagcatgc aaaaggtcaa
    20641 gaattaggct aaaaataaaa aaccaaacga cgggaaagaa aaattactac gtgatgtaga
    20701 ataagccttt ctatcgtttg gttattttat attcaaatat ttgaaataaa taaatcttta
    20761 ctctttgatt tgtaaactga aatgatccac tgtttgtttt actgatgaag cttgatcaaa
    20821 ggaagaagct gagtttgaag ttcataaaaa tcagccatag tgagttttgc ttggatttgt
    20881 ttatcttctt ctacttttta cataattttg aacaggataa gattcaaatg ttttaaatga
    20941 atctttgact agtttgtgac agttgtcgca tatgctcaag cttccttggt cattaaattc
    21001 tgtatggata tttttgttgt tattacatgt acaataatca ggactatcga tcatccattt
    21061 cgtctccttt acaatcatct tgcttcttta ctatagcaga aaagaagggg agaaacaaaa
    21121 gaagatccaa cagacggcac tatagactgt agatgttgcc atcctcatcc ttcaaacggt
    21181 aattaccgat ttttgtaaat atgactgttg tttctttttt attttctttt cctgaaataa
    21241 tatattcgtc atctttggta gtgatcttga tatctgtcag cactgttttt tcttcagatg
    21301 attcagaaga atcagttcct tgtgtctgat acgtgcgtgt ccattccatt tttttatctg
    21361 taaatgtcaa ctgtcccgcg gataaatcca tatctttacg tacttttagc gtatatacac
    21421 tgttggtggt atctgctttt tccgctttta agacagaaga atcggttgtt tcgtccgagg
    21481 attggtcaga gccgccaaac aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa
    21541 taaatagtac gattaatttc ttcaaaatac ttcccccatt tgataggata aagaaattat
    21601 accattggaa tgtaaaactt caaacggatg gaagagaaaa acgcacaaaa cacccacaaa
    21661 tagagtaaat tttagaataa aattttaaaa aggcaaaaaa tccgccactt tcagaaagcg
    21721 ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat taaaaatgaa ccggttcaaa
    21781 tacttgttta agtataatta atagtgagag gaagttgctc tactgaagac tttagttata
    21841 tggatattga tgacaagcaa cactatgcaa agaatgcggt catcataatc gaacaacaag
    21901 aaagagaaat caaatgtaac tggcaggagt gagaatgtat ggcatcgatc cgtgatgtgg
    21961 ctaagatggc tggtgtttca gtgggaacag tttcccgtta cttgaatggt cagcaactga
    22021 aggaaaagaa catgaaaaaa atagcagaag cgatttcagc gttggactat aaagaaaaca
    22081 tcattgctaa agggttgaag aataatcgaa gcttctctat tggacttttg ataaacggga
    22141 tttccagccg ttttgggtcg gaagttgtct cagggatcga aagagtagct gaagaaaatg
    22201 ggtatagctt attattaagc gggtttgccg atcagccaga aaaaattgaa caaaaaattg
    22261 aatacctgat gaagcatgtg atcgatgggc tgattgtctt cttatccgaa gaagagtggt
    22321 ctggatttga aatgctcaca aaaatgagta ttccagttat cgcattgaat tgtccagaag
    22381 gaccagcagg tattgataca atattagtga atgatcgaga aagtgtagca aaagtgatcg
    22441 ctcatcatgg tgaacaagga cataaaaaaa tcggatttat tgctgccact caaacagact
    22501 acgtagcaag agaacgttta gcaggagcca aagaagcggt ccaaaatgat ccatcgatcc
    22561 aattagaggt ctttacagga gactattcta gaaaaagcgg ctattacggg gcaaaagcat
    22621 tattagaaaa aggggcaact gctatttttg tcagcaatta caatatgtcg attggtgcca
    22681 tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga tattgcgttc agtcactatg
    22741 attatttaga taaaaacgat ctgagtatcc ttccgaaaat cacgatcacg ccaccaacag
    22801 ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg aatcaaaaaa gaagaggaag
    22861 aaaatgaaaa agtgattgtg atggaaaata tcatacatgg aatcaactaa aacgaactaa
    22921 aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc cagcaaattt ttggtggggt
    22981 tctgcagcaa gtggcccaca aacagaagga aggatttcag gcgatggaaa aggagagaac
    23041 atttttgatt attggtacaa aaaagaaccg caaaagttct ttgatcaagt aggacctgaa
    23101 aaaacctcgc aagtttatac gaagtatcaa gaggatgtac agttgatgaa acagactggg
    23161 cataattcat ttcgaacatc tatccaatgg agccgtttga tacctgatga atctggaaca
    23221 atcaacgaag aagccgttcg tttctatcat cgttattttg atgagttgat tgaaaacggt
    23281 atcgaaccct ttgtgaatct gtatcatttc gatatgccga tgtatctgca agaaaaaggc
    23341 ggatggttga accgagaaac ggttgaagcc tatgaaaaat atgccaaaag ttgttttgaa
    23401 ttgtttggtt cgaaagtaaa aaaatggttt actcataatg aaccaatcgt tccagtcgaa
    23461 gcaggttatt tatatcgatg gcattatcca gaagagtcag atatgaaaaa agcgatccaa
    23521 gttggctatc atgaagcgtt ggcttcagcg ttagccatca aatcttacca tgaaatggag
    23581 caaggtggtg ttattggtat catcttgaat ctgacaccaa gctatccaag agacgaatca
    23641 aaccctgaag atgttaaagc agcgcaaata gcagatgcgt tcttcaatcg ttcattcctt
    23701 gatccagctg taaaaggcga gtttccaagt gaattgattg agatagtaaa agaacttgat
    23761 ctcatgcctg tgatccatgc agaggatttg acggttatca aagaaaatac agtagatctt
    23821 ttaggtatca attattacca gccacgccgg gtaaaagcaa aagaaacacc aatcgatact
    23881 aaaaacggtc caatgcctga ggattatttc gacaattatg aaatgcctgg tcgtaaaatg
    23941 aatccttatc gcggatggga gatctatgaa caaggaatct atgatatttt gatcaatgta
    24001 cgagatcatt atggaaatat cgactgcttt atttctgaaa acggtatggg tgtagaaaac
    24061 gaagcacgat ttatcaaaga agatggcatg atccacgatg attatcggat cgaatttgtc
    24121 caatctcatc tgaaatatgt ccatcaagcc attcaagaag gtgccaattg tttaggctat
    24181 catatgtgga catgtatgga taactggtcg tggaccaatg catataagaa tcgatatggc
    24241 tttatttcag tagacttagc acaagatgga aaaagaacaa tcaaaaaatc gggtcattgg
    24301 ttcaaagacg tatcagataa aaacggattt gaggcttaag aaagaatagt tgagagcaaa
    24361 tgcgtgaaat tctccattct taccattttg tgaaaaagaa gttcaattat ttacgatctt
    24421 tttcaacttt tttgtctttc tatttcattt tttgtatata atagaatagt caaaattaaa
    24481 taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct tctcttttgg ttattcggtt
    24541 ttttcttact gcttatttta tccgcagcat tttcaggaaa aatgacagca gatcttgctc
    24601 agaaagaaac agaaaaaatc gtagcaagca aagaatcagt agataatttc aaaaaagaat
    24661 tctcaactta ttggtgggcg gagtataaaa taactgccaa ttacgaaaaa ggcgaattgg
    24721 acatttattt gccaaaagga aaagaagaaa atcaatttat taatatcgat gacgtactta
    24781 gtgcgatttc tctcagacaa tatcaattta aagacaagca tttgaccgtt ttgcttttga
    24841 ctcatgatgg cgacaaagta gcccgtttga tcaccgctta ttctgaaaaa gtagacaaaa
    24901 aagtggtcgc cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa
    24961 ctgtctcttc tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct ggcagtggac
    25021 aaacgataac gggaagtgaa gaaaatcaag cctcaattat tacgaatgag tcatcacaag
    25081 gacaatatca atcctatgga caatgaaaaa
//
LOCUS       DAFFQY010000037        24665 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFFQY010000037 DAFFQY010000000
VERSION     DAFFQY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.037"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            236..1042
                     /locus_tag="LRB02_001404"
     CDS             236..1042
                     /locus_tag="LRB02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659612.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="LRB02_001405"
     CDS             1054..2274
                     /locus_tag="LRB02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659613.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="LRB02_001406"
     CDS             2264..3850
                     /locus_tag="LRB02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4659614.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="LRB02_001407"
     CDS             3889..6027
                     /locus_tag="LRB02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4659615.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="LRB02_001408"
     CDS             6315..7706
                     /locus_tag="LRB02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL4659616.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="LRB02_001409"
     CDS             7765..8574
                     /locus_tag="LRB02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4659617.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..9535
                     /locus_tag="LRB02_001410"
     CDS             8546..9535
                     /locus_tag="LRB02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4659618.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKK
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9649..10644
                     /locus_tag="LRB02_001411"
     CDS             9649..10644
                     /locus_tag="LRB02_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4659619.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10648..11790
                     /locus_tag="LRB02_001412"
     CDS             10648..11790
                     /locus_tag="LRB02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBL4659620.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11787..13214
                     /locus_tag="LRB02_001413"
     CDS             11787..13214
                     /locus_tag="LRB02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL4659621.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13281..14570)
                     /locus_tag="LRB02_001414"
                     /pseudo
     CDS             complement(13281..14570)
                     /locus_tag="LRB02_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LRB02_001415"
     CDS             complement(14750..15160)
                     /gene="tagD"
                     /locus_tag="LRB02_001415"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HBL4659622.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15286..16677
                     /locus_tag="LRB02_001416"
     CDS             15286..16677
                     /locus_tag="LRB02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL4659623.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16810..17757
                     /locus_tag="LRB02_001417"
     CDS             16810..17757
                     /locus_tag="LRB02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL4659624.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17797..18957)
                     /locus_tag="LRB02_001418"
     CDS             complement(17797..18957)
                     /locus_tag="LRB02_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL4659625.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19122..20297
                     /gene="mltG"
                     /locus_tag="LRB02_001419"
     CDS             19122..20297
                     /gene="mltG"
                     /locus_tag="LRB02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL4659626.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20433..20912
                     /gene="greA"
                     /locus_tag="LRB02_001420"
     CDS             20433..20912
                     /gene="greA"
                     /locus_tag="LRB02_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL4659627.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21080..21811
                     /locus_tag="LRB02_001421"
     CDS             21080..21811
                     /locus_tag="LRB02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL4659628.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21808..22875
                     /locus_tag="LRB02_001422"
     CDS             21808..22875
                     /locus_tag="LRB02_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4659629.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            22882..23514
                     /locus_tag="LRB02_001423"
     CDS             22882..23514
                     /locus_tag="LRB02_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659630.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23654..24319
                     /locus_tag="LRB02_001424"
     CDS             23654..24319
                     /locus_tag="LRB02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL4659631.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24347..24640
                     /locus_tag="LRB02_001425"
     CDS             24347..24640
                     /locus_tag="LRB02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL4659632.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8419 a         3663 c         4780 g         7803 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa aaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata
     9421 ttcaaaaata agaagtaaca atgagatatc aattgctaaa aaattagctt tatttttata
     9481 cttaattaat cctaaaactt attttatttt atataaaaaa gttctaaaaa aataaagttt
     9541 ttttcgatat gttatttttt atgtttatta acttcggaag actaaaattg ttactggtaa
     9601 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
     9661 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
     9721 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
     9781 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
     9841 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
     9901 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
     9961 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
    10021 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
    10081 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
    10141 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
    10201 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
    10261 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
    10321 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
    10381 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
    10441 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
    10501 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
    10561 ttaataaata aaaacttgcc ttactatatg tcaagtagta agatagcaag taaattcttg
    10621 attaagatag aatctgtgag gtaagagatg aagaatataa aaaaattttt ttcaaggaat
    10681 attttcccgg tttttattaa aatattacct attcaaaaaa atttaattgt attttcaagt
    10741 tttggcgggc gtaactattc tggaaatcca agagttattt cagagaaaat tcatgaaaaa
    10801 aaattgaatt acagaattat ttggttaatc aaaaaagaag cgattataac tgatttacca
    10861 gattatatta aaagaattga ctccgattct tttttagctg ccttttattt atatacagct
    10921 aagtattgga tagacgatag tcggaaaacg attaagtatg caaaaaggaa aaaacaactt
    10981 tattttcaaa catggcacgg aacgccttta aaaaaaattg aatttgatgc aaaagataag
    11041 ttacctaaac gttacctaag ctatgcgaaa aaagatagtg agtcaattac atatttatta
    11101 tctggtaatc gctatagttc taagaagtat gtttcagcat tcaatatcgt tccttctaaa
    11161 atattagaag taggaactcc tagaaacgat gaattagcct ctagtaaaga gcaagtgttt
    11221 gacttgaaaa agaaacaaat caatgtctta tttgctccaa cttttcgaaa taatattgag
    11281 gacaacggaa ttacgcaact agaatggtta ggtgtggata acttacgtga attttttaaa
    11341 aagcaacaac aagaattaaa ttttatgact aaatttcatc ctaatgttca tagtaaatta
    11401 gcaactgatt ctaaaagtat agaatatatg aaaaaacatc aaattacttt aattaatgat
    11461 gggattcctc ttgaaatgct ttttgaagag atagatgttt taatcacaga ttattcatct
    11521 atttttttcg attttgcgct aacaaaaaaa ccaattattc tttttaacta tgatgaaaaa
    11581 gaatatagct tagaaagagg tttttatata cagttaagtg agttaccaat tataagcgta
    11641 caagatagtt atggccttgt tgatgttttt aaaaaccaac aagaaaagct tttgaaaagt
    11701 tctcaagagt tacttgatta tattggaaat tatgaaacag gagcaagtac agaaaaggtt
    11761 cttgagttga tagagaggga tataaagtga aaaaaacagt aaaagatata ttttataatg
    11821 cgatatatca agtctttttg attgttttac ccttacttac tatacctata cttagtcgta
    11881 gaattggttc aactggttta gggatttatg ggtatgtttt ctcaatatca cagtttttaa
    11941 tgactgtcat tgctgtggga atgaatcctt ttcgtattcg aaatatcgct aaatcaagga
    12001 aagataaaaa agcactatct ttacaattct ggaatattta ctttattcaa tttttaatag
    12061 gcttgagtgt ttcctttctc tatatagcct atatttattt ttttgatgta aagtataaaa
    12121 atttatactt aattcaattg atatttattg tagggctaac attagatatt tcttggtttt
    12181 ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa tacagttatc aaattatcat
    12241 cggtattgtt aataatcatt tttgtttcaa ctaaagatga tttgtggatt tacgtgttca
    12301 ttacttcaat tactaacttt ttaggaagtt tagttttctg gttaagtatc cacggaaaag
    12361 taggtaaacc aatctttaac tctaatgttt ttaaacagtt gtggaaacca gcttttatta
    12421 ttttgactcc tcagttgttt atgcaagtgt acacaacttt agacaaaacg atagttgggt
    12481 attttgtgga tccaacggaa ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta
    12541 tattggcagt cttaacatca attactattg ttatgttgcc taaaatatct aaagcacagg
    12601 cagaggggaa atcagaaaat attttagttt acacaaaaaa atcttttgat tacacgatta
    12661 tcattgcatt aattcttttt agtgtggtgt ttatcaatac aaaagaattt gttccatggt
    12721 tttttgggga gaatttcaaa ccgatgacaa taaacatgct tatttcttgt ctaattatag
    12781 ttgttagtcc aattggtgga gtttttgcta accaatttgc aattgctata gaaaaagata
    12841 aggagtatgc ttatccaatg atcattggtg cgattgtaag tattattgga aatgtagctt
    12901 ttgtgcctac ttatggcgct gtagcggcta cgatcgtact cgtggtagta gaattattag
    12961 tctttttctt tagagtattt tttgtacgaa aagaaataaa aatatcctat ttctttggga
    13021 agaacgttat cttatttttt atagtcaatc ttactttgac tacttgggcc tattactttc
    13081 ttccacaatt attctcttct tcatttttag atatggcaat taaatcaata atgattttca
    13141 ttatttatct tccgtttgtt tattttcttt ttccagatat acgaaatgaa ataagaaaga
    13201 ttactcgaaa ataaaataat gttgtaaaaa agtagttctt tgtcaaatcg ggctgataag
    13261 gtcatttatg aacaacaaag ttagtaagag ttactagctt ttaatgcata gctgattaac
    13321 ctgttgatta gaaaaatcca caagcgcata ttggtaaaac tgcgaaaccc ataagcaaat
    13381 cgtttcaatg ttttgatatg tgtgcgaaga ttctctaatt ttgttattag aatacttttt
    13441 cgaaaatgct agttaaaagc ctttttcaga tatgaaaaag gctttttgag atagtgaagt
    13501 ttcgagggaa cttctttggt cgtttctcga agtagctagc tgtttcttgt gataattgct
    13561 aagcagcttt tgataaactg cttagcaatt ttaagttcat aagagattga gggtagacga
    13621 tcaactactt cactttctgt taaatagcgc cattgaaaat tctgccattt cttgaatgtt
    13681 gtgtaatcca actcgtcatc tgattttaga agtaatttat acaaaggttt caattgtcga
    13741 tagtacttag cttgtgaagt gtctttttga ttcaactgct tcatcacacg ttttcgaaca
    13801 cgatcgaaaa acttgttcaa gtgttgtcca atgtgaaagc aatcaatgat gatttgggcg
    13861 tttggaaagg actctttgac taatgaaaaa taaggggcat tcatatttgt gacaatgtat
    13921 cgaaccccca tatgagcttt tcttgtaaaa cgcatgaaat aagcaatcaa atcttttttt
    13981 ctccggctgg acaaaatgtc aaagagcttc cctgtctctc tattcataca agaaaaggag
    14041 aactttccat gggaaattga aagagagcga aactcgtcga atagtaaaca atgaggtaaa
    14101 tagtctagca tctgtcgcag cattgccgat agagcagata atgtccgttg aaatgtcgtt
    14161 actgaaagat cacaacgttt cgtaagtgtc tttaacgctt gacgctcggt caactcttct
    14221 aatatcgtgt aatgaacttg tttcgaaata aaacatgaag gaacgacaag atacgtatga
    14281 gcagtaaaat attttccgca tgattggtag tggtaatgtt gttttctaag acgcaaatag
    14341 gtttatttat tcccactgcg atttaagata atcattgaaa tcttcttgcc gtttttcacg
    14401 agctgcttcg cagtttcttt acaaaataga caatgtctaa aagtgcgaac taatgtacct
    14461 tcgattacga tggctttctt gtccttataa aaagtttttt gaaaagaatt aatttcaata
    14521 tttttatctt taatagtgaa gtgctctttg atagaacgag aaatggatat acaaaatctt
    14581 cctttcagat tgatgtttta gacgactcta ttttatcgaa atttttgtat gtctgttttt
    14641 tttacaagaa aatctaggat cgacagaaaa ttctatcagt cctagaaatt atagagccat
    14701 ttttttacgt cttatccaga tatattgttc tttcattgtt taaaaaatat tatttatcac
    14761 tatttaaaga attaagttcg ttctttattt gagttgtaga gatctcaggt gttcttggta
    14821 aataaattac ttgtgcatct gttagttgtt caagttcatc aaattgtcca gcccaatcat
    14881 cgcccatcac gaaataatct gcatggtatt cttttatatc ttttactttt tgttcccaat
    14941 tttcttcagg aataactaaa tcaacgtagc gaatagcttc aagtaactgt tttctttttt
    15001 cataactaaa ataacatttt ttttgtttcg cattccaatt gaattcatct gtagaaaggg
    15061 caacaataag ataatctcca tattgttttg ctcgtttcag taaattgata tgcccgtagt
    15121 gaagcaaatc aaaggtaccg taagtaatta ctcttttcat ctttcacctc tcgaaattca
    15181 ttaatataca ttgttagttt agcatttttt tgttagaatt gaaagaacca gtttatccta
    15241 aaaaggataa gaaatgttta tgagaaatag gtggatggag gaaaaatgtt gaatgaaaaa
    15301 tttttaaaat atgtgaaagg ttggtatttt atttattttg gttttattct aatgttacga
    15361 gcaataggcc cgtatttact attacctact agatatgata ctttactttt ttggcctgct
    15421 ggaatagcgg gagttgtctt gatcattata gatgcaatta ttgcatttag agaaaaaaat
    15481 cttaaaaaat atgattggct attaattgct tttattggcg cgatgctagt aagcagttta
    15541 atgaatttaa aatatggata tagcgaaaat atcaagttaa tcatgtggca attaatcttt
    15601 ttctttatga tttatcagtt tggcaaagac tacagtagta actttttttt caaagtattt
    15661 acttggatac tttcattggt atggttttta gctaatttag tatcattata ctggtttgtc
    15721 attcaatatg gcataaaaat tccaataaaa tataagtatt acccagttcg tttagggtgg
    15781 ttaggcaacc gtttatttgg tgtatataca gatccaaatt ttggtgcggt tatatcatta
    15841 attgtcatca ttattgctat atattatttg gtcacttaca agcatttaaa tcttctattt
    15901 aaaatatttt ttgcaattaa tatttttatt caattctcgt ttattgcttt atcgggctca
    15961 cgcaccacat tagtttgttt attaatagca acttttgttt ttagtttctt tacaagttat
    16021 agtagtcgtt ttcttaaaac taatacttta aaaagatggc taactgcatt agtagtagga
    16081 ttgctaagta taattgcgat gtattatgta acagatataa ctgctcaagc atgggcacat
    16141 gttccgcata ttacagaaat aaaaaaagat atatctgatt catcaggaac aactagcagc
    16201 aataaagttg atttaaaacg ccctgacact gatggcgggg atgtttctaa tttaaggttt
    16261 ggactttgga aaagtgcaat tgaaatattc aagagtaagc cgatatccgg cggttctcca
    16321 ggatactata ttgaatacgc acatgataaa ctaccatata cattaatggg aaaagatact
    16381 ttaactgctc ataattttat tttcttagtt atggctgcta caggaggatt aggtctattg
    16441 acattgttaa ccttcttatt tatccaatgc tttaaaactt tgcgttattg tttcacacaa
    16501 ccaaatctag taaaagataa gcactttttt gctattttaa tgattcttac aatagcagtt
    16561 tcggcattat ttatcacaga actcattttg gttagtacga taggttcatt tattttttgg
    16621 ggatatctgg gttctatcca aaaaactata tctaaagaag aaggagtaac taattgaaac
    16681 tatttttagg tatataaagt agaaaagaaa actagttatc ccaacttcaa caaattttat
    16741 agtttttaca atataaaaat ggtaaactaa ctttattaag tatgagaatg cataattgga
    16801 gtgaatttga tggatactat attgattact ggtggagctg gttttatcgg ttcaacgcta
    16861 gcgaattatt tgggaaaaga aaacaaaatc gtagtagtcg acgatttatc aatgggaaaa
    16921 aaagaaaact tggatatgga gaaacaagtg actttcattg agggggatgt atcagatcct
    16981 caattgatgg aaagaatcat gaaacagtat cagtttgcct atatttttca tttagctgct
    17041 gtagctagtg tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac
    17101 agtgcgcttt tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta
    17161 tttagttcat cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg
    17221 gtcatccgtc ctttaacacc atatgccatc gataagtttg catcagaaca atatgttttg
    17281 gattactgtc atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt
    17341 ccaaatcaaa atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac
    17401 aaaaaacaat tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct
    17461 agagacttcg tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa
    17521 aaagctttag gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg
    17581 attcattcaa ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt
    17641 tcaggggata ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa
    17701 cccaaatttg atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac
    17761 aaaaaaccag catcctgctg gtttttttgt ctcgacttag ctttttaaat taacaccttc
    17821 catcagttcg taaatctgat ttcttaaatc agtggccggc aatttgatgt ttttatcaga
    17881 ttggatatta ctttccatga cgattgcatc ttttgtgtct tttttcatcg ccgctactgc
    17941 atggaatcga ttgatatttc cctgagcaag gatataatca tttccactgt aaacaccgcc
    18001 gctgtaagga tgtttatcac cattattttg ccacaatgag aaaagtagtt cttttggtat
    18061 aatatcgcca gacagaacac tggtaaagaa tttgttcaag tcataaacag acatacttat
    18121 atttcctgtg cctaattcat ttcggatgtc tgtttcaggc tcgaccaatg cttttgaata
    18181 atcgtcagtt gcagacattt ggtaagatac agcatgctga ggggaatttt ttacattttc
    18241 ataaaaatct gtatgttcta gatggagtgg ccgaatgatt tcttttttta aaagagtttc
    18301 ataaggctga ttcattagtt tgatcaatac ccctgtaagc aatgtataat ttaatggttc
    18361 ataatgatac gtatcataac cagtaaattt caaatgatga agggcaaact gaatgatctc
    18421 actatctgac ataggttttt ttggagaagc ggttcgatga agaccagagc gcatgtaaag
    18481 catatttttg attgagattt gctgacttcc aggaatctga ggataaaatt cagatagctt
    18541 tgtgtcaaaa gttatcttgc cagattgaat ttgttttagg ataagtaaag cagtcatcgc
    18601 tttttggatc gaaccgattt gatagtaaag agaaggagaa ttcgattctt ttatgcgtag
    18661 atttgcgtac ccatatcctt tttctaatat cactcgatta tttttgcgta cataaatcga
    18721 accgatataa tctgattttt gcagtaattg atcaattttg ctggcaagca tctgctcatt
    18781 agatgttaaa ttggaagcaa gttgtgcggg ttccgtttta taaatttgtg gttgaggttg
    18841 ttttgttaat tgccatcctg taatgatgcc tgttgaaaag aagctgacag ccagcaacaa
    18901 aactgcgaga aaagaaagag agatctgctg ttttttttga tgttttcctt ttgccatatt
    18961 aaatcctttc tatccatttt taactatttt tcattgtaat cctcaaaaag agcatttgcc
    19021 acttacaatt ttgacataat atttggtaaa ataattcacg gcagtagaaa tttcatgtat
    19081 aataaacaga gttacgaaga tgatagtaag gaggagttgt tttgtcagga aacaaacaac
    19141 acccaagcaa agacaaaaaa gaagccagta aggcagctac agcagatata atcgacaagc
    19201 ggagaaatct gcgtaaaaaa gaagataaga tcgtgaggaa gatcatttta gtgatcgctc
    19261 ttacattatt gattattggc ggatttctcg ggtttaccgt atatcgttat gtagatagcg
    19321 ggttgaagcc tttagataaa tcggacgacc aattagtcca agtggaaatc ccaaacggct
    19381 cttctaataa gcagattggc gaaattttgg agaaagacaa tattattaaa agtgggattg
    19441 ttttcaatta ttatacaaaa tttaaaaact taactggctt tcaagctggt tattatcagt
    19501 tagcgcctaa tatgactctt gatgagattg gcaagcagtt gcaagaaggc ggcacatctg
    19561 agccaacaaa agttgcagat gggaaaattg ccattccaga aggctacgac atcgatcaga
    19621 ttgctgagcg ggtagcgaag gtaacaggta aagacaagaa agaattttta gatttagtaa
    19681 atgatgagac tttctttaac gagttgtatc aaaaattccc agaattgttg gaaagtgcct
    19741 ctaaagctga gaacacaaaa taccgtttgg aaggctactt attcccagct acttatgatt
    19801 actacacgag tacttcgctt aaggatcttg tgattgagat ggtgaacaag acaaatacgg
    19861 tcatgcaaaa ctattattca gctatcaaac agaaaaattt gactgtacaa gaagttttaa
    19921 ctttggcttc tctagttgaa aaagaaggcg taaaagaaaa tgatcgtaaa aatattgcac
    19981 aagtcttctt taaccgaatc aaggcaaata tgccacttca gtcagatatc tctgttttat
    20041 atgcattagg agaacataaa gaattggtga cgtatgaaga tacagctgta gattcaccat
    20101 acaatttgta taccaatacc ggctatgggc ctggtccttt tgacaatcca agcgaaaagg
    20161 caatcaaggc agtattagaa ccagcagaaa atgactatta ttatttcgta gcagatacga
    20221 gcactggaaa tgtttatttc gctaaaacgt atgaagaaca tatggaacta gtgcaaaaat
    20281 atgtaaataa ttcttagaaa aacaggggct agtcattgat catttttaga aaaacattta
    20341 cattttaaga aaagcgtggt aatctttttt ggactatatt cctcaaaaga ttaccttttg
    20401 cggtatttta tcgaaaaaag gagacgttac acatggtaga gaaagtttat ccaatgactc
    20461 tagaaggtaa agaaaaatta gaaaaagaat tagaagaact aaaaacagtt aaacgcggag
    20521 aaatcattga acgaatcaaa atcgctcgcg gtttcggaga tctttccgaa aactccgaat
    20581 acgaatcagc taaagatgaa caagcatttg ttgaaggaag aatcacaaca attgaaaata
    20641 tgatccgttt tgcacaaatc atcgataatg acggcgtaga tgcagatgag gtatcgatcg
    20701 gtaaaacagt tacgttcatc gagttgcctg atggagatga agaagagtac acgattgtcg
    20761 gaagtgctga agcagacccg ttttctggaa agatttccaa tgattctcct atcgcgcaag
    20821 cattgattgg caaacgtaaa aatgatgaag taaccattgc tacaccaggt ggcgatatgt
    20881 tagttaaaat cgtcaaagta gaacaagcgt agtctgactg aaagatctag agagttcatc
    20941 ttactgatga cgcactctag atcttttctc tttgagtatt gcaggtttca ctgaacgatc
    21001 ttacatctaa agtcctatag ccaacagtca aaaaaatagg tatgatgaaa tgagaatgtg
    21061 tcaggaggga gaaaaagcga tgaatagttc ttggcgtttt tttgtggtag tcgaagcact
    21121 gctgctgtta tttgctgttt ggcaaatcgt aaacaatact ggacttttgc ttttagtaat
    21181 attcggtatt ttcaatatat atctagctat gcgcaaatat cctcgtacta aatttcaaaa
    21241 ttttcaattg atcttaggga gcttggtcat tttttttagc ttagtcaaca gtcctgcttt
    21301 atggatgatg gttgtattgg ccgttttatt tatcgggctg aaaggtgtag agatttcagg
    21361 aatagatttg acaaaaaatg ctttttggcg aaaaaaacag atcatgatgg tccaaacaga
    21421 gcaattaaaa acacataata atgagcgtaa aagacagcaa ttatttggta accaacgtat
    21481 tggaaacgac gtctatgaat gggatgatat caacattgcc atcatttctg gtgatactat
    21541 catcgatttg ggtaatacac ttttgccaaa agatgataat attgtgattg taagaaaagg
    21601 gataggcaga acacggatct tagtacccct gggagtggct attcgtttag agcatgcgac
    21661 attagtagga aatgtcttgt ttgaagaaga gcagttttcc ttaaaaaacg aacagatcaa
    21721 aatctgcagc agtgattatg atgaaaatcc tcgcagattg aagatcatta ccaatacctt
    21781 ggttggagat gtcgaggtga tacgaatatg atgggaagaa tatccaaagc aatgctagct
    21841 gtttattcgg ggattgcggc tttccttatt attctatttt cacttttcac ttatttttat
    21901 gccagtaacc aaagtcattg gtggggagaa ttgctgcgtg cgcgtttatt atacgttccg
    21961 cttatcttcc atttgatggc aatttcatta ggtgtaggat tgatcgtatt tctattgtta
    22021 tcacttattc aaaaggcaaa atacgggaaa atcgaagaaa agcttcgtgc actttcttct
    22081 ggcaattatg aatccaaact tttgcatcag ccgatcccaa gcgcgtcgga tgatctgtac
    22141 atcaaagata ttgatatgga aattgctaaa ataaaagaaa aaatgatcga agtatccagt
    22201 gaattacaga ttatgactag ccgtcctcaa tatgtagatg gacaaaccaa agaagagatt
    22261 ttagaattag aaaggcatcg attagctcgt gagcttcatg attcagtttc acagcaatta
    22321 tttgcagcga tgatgatgat gtcggcattg acggagcaag cagaaaaaag tgagacacca
    22381 gaaatgttcc gtaagcagtt gaaaatggtg gcagagatca tcaacgcttc ccagtctgag
    22441 atgcgtgcgc tgcttcttca tctgcgtcca gtcaatttag aagaaaaaag tttaaaacaa
    22501 ggcattgagc agttattgaa ggaattgcag aataaaatcc agatttcgct aaaatgggat
    22561 gtagaagatg taaaattaac tagttccatt gaggatcact tgttccgaat cgttcaagaa
    22621 ttgttatcaa acacattaag acatgctaag gctaatgaat tagaagtcta tttgcataaa
    22681 atagataaca atcttttatt acgtatcatt gacgacggga caggatttaa tatgaatgaa
    22741 accaaaacgg gaagttatgg attgaacaat atcaaagaac gagtagctgg tattggtgga
    22801 acagtaaaaa tcattagttt taaagggcaa ggtacgagcg tggagatcaa ggtccctttg
    22861 atgaaggagg catagataaa gatgataaaa gttttattag tagatgacca tgaaatggta
    22921 cgcttaggcg tctcttctta tttatctatc caagaagaca ttgaagtgat tggagaagct
    22981 gaaaatggac gtcaaggcta tgaaaaggcg atggcacttc gaccagatgt cattttaatg
    23041 gatctagtca tggaagaaat ggatggcatc gaatcaacaa aagcaatctt aaaagattgt
    23101 ccaaaagcca aaatcattat cgtcacaagt tttattgatg atgaaaaagt ttatcctgca
    23161 attgaagcag gagcagcagg atatttgttg aagacgtcta ccgcacatga aatagctgat
    23221 gctataaggg ctacgcaacg cggcgagcga gtattggaac ctgaagtgac aacaaaaatg
    23281 atggaaaaaa tgagtcggcg aaatgatcca gtattgcatg aagaattaac gaatcgagaa
    23341 aatgaaattt tgatgctgat ttctgaaggg aaaagtaatc aggaaatcgc ggatgaattg
    23401 tttatcacac tgaaaacagt taaaacgcat gtgtcgaata tattagctaa gttagaggta
    23461 gaagaccgaa ctcaagcagc tatctatgcg tttaaacatg gtttggtgaa ataaaaacga
    23521 tgataaagag gataatcaaa agctgagaaa ataactatta tttttcttgg cttttttaca
    23581 ataatgatgc ttgcttttgt ttttggaaag gctttgttat actgagaaaa gaagatacta
    23641 aaggaggaaa atggtgaaac aaaattttgc aattgtaggc cttggacgat ttggcggaag
    23701 catctgccgt acactgatcg aagctggaca agaagtactg gctattgaca gtaatgaaga
    23761 tcgagtcaat gaatatatga atatagccac tcatgctgtt gtggcaaatg cacaggatga
    23821 gatgacactt cgttcattag gtataagaaa tttcgatcat gtaattgtcg ctattggaga
    23881 agatatccaa gccagtatct tagttactct gatggtaaag gaaatgggcg taccgaatat
    23941 tttagcaaaa gcccaaaatg agtaccatgc ccgtgtacta gagaagatcg gagcagatcg
    24001 tgtcgtacac cctgagcgag acatggggat acggattgca cataacttag tatcaaagaa
    24061 tattctagac tacttagagc tgtcagataa atattcttta gcagaaatcc gagtctccaa
    24121 tcctaaattt ttcaacaaaa cattagctga attaaatttc aggcaacgtt ttgacttaac
    24181 agtagtcgcc atacgaagag tagatcaaac ggttatcgct tctcctgcag cagatgaata
    24241 tgttcgcgaa aatgataatc tactggtgat tggagagaca ggcgatgtgg atattctaga
    24301 cgataagatg aatcaataga aagaaaagag aaagtaggtt gttgggatgg aattaacggt
    24361 aacgccaaaa gcacaaaagt ggtttgactc agaaattgat ctgccagaaa cttacggaat
    24421 acgttttttt ggaaaagtct acggcaaaac agaggttcat gatggttttt cgattggaat
    24481 gtcagttgaa cagccagaac atcctgtaaa gaaagtggaa atggatggga cacttttttt
    24541 tatcgaggaa gcagatgaat ggttctttaa aggatatgat ttacttgttg attatgatcc
    24601 caacctggag gagccaacct atcactttaa aaaacaataa aaaaagagag ggtgacacaa
    24661 gtttt
//
LOCUS       DAFFQY010000038        24033 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFFQY010000038 DAFFQY010000000
VERSION     DAFFQY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.038"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(24..722)
                     /locus_tag="LRB02_001426"
     CDS             complement(24..722)
                     /locus_tag="LRB02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL4659633.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LRB02_001427"
     CDS             complement(715..2067)
                     /locus_tag="LRB02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL4659634.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LRB02_001428"
     CDS             complement(2290..3690)
                     /locus_tag="LRB02_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL4659635.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LRB02_001429"
     CDS             3851..4588
                     /locus_tag="LRB02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL4659636.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LRB02_001430"
     CDS             4713..5192
                     /locus_tag="LRB02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL4659637.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LRB02_001431"
     CDS             5189..5956
                     /locus_tag="LRB02_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659638.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LRB02_001432"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LRB02_001432"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL4659639.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LRB02_001433"
     CDS             complement(6859..7461)
                     /locus_tag="LRB02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4659640.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LRB02_001434"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LRB02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LRB02_001435"
     CDS             8142..8726
                     /locus_tag="LRB02_001435"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL4659641.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LRB02_001436"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LRB02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL4659642.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LRB02_001437"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LRB02_001437"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL4659643.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LRB02_001438"
     CDS             10627..10806
                     /locus_tag="LRB02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659644.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LRB02_001439"
     CDS             10828..11853
                     /locus_tag="LRB02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL4659645.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LRB02_001440"
     CDS             12028..13272
                     /locus_tag="LRB02_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL4659646.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LRB02_001441"
     CDS             13425..14372
                     /locus_tag="LRB02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4659647.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LRB02_001442"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LRB02_001442"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL4659648.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LRB02_001443"
     CDS             complement(15274..15516)
                     /locus_tag="LRB02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659649.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LRB02_001444"
     tRNA            15637..15709
                     /locus_tag="LRB02_001444"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LRB02_001445"
     CDS             complement(15828..16466)
                     /locus_tag="LRB02_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4659650.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LRB02_001446"
     CDS             complement(16589..17221)
                     /locus_tag="LRB02_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL4659651.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LRB02_001447"
     CDS             17572..17871
                     /locus_tag="LRB02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL4659652.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18366..20531
                     /gene="acm"
                     /locus_tag="LRB02_001448"
     CDS             18366..20531
                     /gene="acm"
                     /locus_tag="LRB02_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL4659653.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20803..21288)
                     /locus_tag="LRB02_001449"
     CDS             complement(20803..21288)
                     /locus_tag="LRB02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL4659654.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21378..21723
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21768..22409
                     /locus_tag="LRB02_001450"
     CDS             21768..22409
                     /locus_tag="LRB02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4659655.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22428..23063
                     /locus_tag="LRB02_001451"
     CDS             22428..23063
                     /locus_tag="LRB02_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4659656.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23081..23896
                     /locus_tag="LRB02_001452"
     CDS             23081..23896
                     /locus_tag="LRB02_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4659657.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRFDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8319 a         4090 c         4776 g         6848 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg
    18061 tattttatga aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa
    18121 tatagattga aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat
    18181 ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt
    18241 tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa
    18301 gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag
    18361 aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa
    18421 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
    18481 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
    18541 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
    18601 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
    18661 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
    18721 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
    18781 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
    18841 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
    18901 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
    18961 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
    19021 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
    19081 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
    19141 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
    19201 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
    19261 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
    19321 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
    19381 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
    19441 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
    19501 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
    19561 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
    19621 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
    19681 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
    19741 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
    19801 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
    19861 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
    19921 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
    19981 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
    20041 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
    20101 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
    20161 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
    20221 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
    20281 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
    20341 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
    20401 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
    20461 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
    20521 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
    20581 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
    20641 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
    20701 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
    20761 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
    20821 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
    20881 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
    20941 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
    21001 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
    21061 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
    21121 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
    21181 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
    21241 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
    21301 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
    21361 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
    21421 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
    21481 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
    21541 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
    21601 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
    21661 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
    21721 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
    21781 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
    21841 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
    21901 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
    21961 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
    22021 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
    22081 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
    22141 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
    22201 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
    22261 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
    22321 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
    22381 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
    22441 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
    22501 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
    22561 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
    22621 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
    22681 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
    22741 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
    22801 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
    22861 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
    22921 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
    22981 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
    23041 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
    23101 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
    23161 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
    23221 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
    23281 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
    23341 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
    23401 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
    23461 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
    23521 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
    23581 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
    23641 gaaaagcaga cggttcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
    23701 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
    23761 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
    23821 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
    23881 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
    23941 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
    24001 acctcttggt aaacggtgtt caaaagccag ttt
//
LOCUS       DAFFQY010000039        23858 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFFQY010000039 DAFFQY010000000
VERSION     DAFFQY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.039"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(196..501)
                     /locus_tag="LRB02_001453"
     CDS             complement(196..501)
                     /locus_tag="LRB02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL4659658.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(526..957)
                     /gene="ruvX"
                     /locus_tag="LRB02_001454"
     CDS             complement(526..957)
                     /gene="ruvX"
                     /locus_tag="LRB02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL4659659.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(954..1220)
                     /locus_tag="LRB02_001455"
     CDS             complement(954..1220)
                     /locus_tag="LRB02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL4659660.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1475..2200)
                     /locus_tag="LRB02_001456"
     CDS             complement(1475..2200)
                     /locus_tag="LRB02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL4659661.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2561..2631)
                     /locus_tag="LRB02_001457"
     tRNA            complement(2561..2631)
                     /locus_tag="LRB02_001457"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Gly,seq:ccc)
     gene            2748..3500
                     /locus_tag="LRB02_001458"
     CDS             2748..3500
                     /locus_tag="LRB02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL4659662.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3516..3995
                     /locus_tag="LRB02_001459"
     CDS             3516..3995
                     /locus_tag="LRB02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL4659663.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4246..5826)
                     /locus_tag="LRB02_001460"
     CDS             complement(4246..5826)
                     /locus_tag="LRB02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL4659664.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5819..6562)
                     /locus_tag="LRB02_001461"
     CDS             complement(5819..6562)
                     /locus_tag="LRB02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659665.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6738..7127)
                     /locus_tag="LRB02_001462"
     CDS             complement(6738..7127)
                     /locus_tag="LRB02_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBL4659666.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7328..7513)
                     /locus_tag="LRB02_001463"
     CDS             complement(7328..7513)
                     /locus_tag="LRB02_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBL4659667.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFS"
     gene            complement(7761..8597)
                     /locus_tag="LRB02_001464"
     CDS             complement(7761..8597)
                     /locus_tag="LRB02_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL4659668.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8608..9537)
                     /locus_tag="LRB02_001465"
     CDS             complement(8608..9537)
                     /locus_tag="LRB02_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL4659669.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9729..10082
                     /locus_tag="LRB02_001466"
     CDS             9729..10082
                     /locus_tag="LRB02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL4659670.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10156..10812)
                     /locus_tag="LRB02_001467"
     CDS             complement(10156..10812)
                     /locus_tag="LRB02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL4659671.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11038..12972
                     /locus_tag="LRB02_001468"
     CDS             11038..12972
                     /locus_tag="LRB02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4659672.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13058..14281)
                     /locus_tag="LRB02_001469"
     CDS             complement(13058..14281)
                     /locus_tag="LRB02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4659673.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14275..16173)
                     /locus_tag="LRB02_001470"
     CDS             complement(14275..16173)
                     /locus_tag="LRB02_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL4659674.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16167..17285)
                     /locus_tag="LRB02_001471"
     CDS             complement(16167..17285)
                     /locus_tag="LRB02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4659675.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17368..18201)
                     /locus_tag="LRB02_001472"
     CDS             complement(17368..18201)
                     /locus_tag="LRB02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4659676.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18194..19111)
                     /locus_tag="LRB02_001473"
     CDS             complement(18194..19111)
                     /locus_tag="LRB02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4659677.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19338..20462)
                     /locus_tag="LRB02_001474"
     CDS             complement(19338..20462)
                     /locus_tag="LRB02_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4659678.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20645..21553
                     /locus_tag="LRB02_001475"
     CDS             20645..21553
                     /locus_tag="LRB02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659679.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21694..23307
                     /locus_tag="LRB02_001476"
     CDS             21694..23307
                     /locus_tag="LRB02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4659680.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7430 a         4655 c         3993 g         7780 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
      241 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      301 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      361 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      421 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      481 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      541 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      601 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      661 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      721 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      781 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      841 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      901 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      961 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     1021 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
     1081 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     1141 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1201 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1261 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1321 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1381 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1441 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1501 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1561 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1621 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1681 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1741 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1801 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1861 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1921 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1981 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     2041 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     2101 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     2161 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2221 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2281 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2341 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2401 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2461 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2521 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2581 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2641 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2701 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2761 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2821 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2881 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2941 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     3001 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     3061 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3121 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3181 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3241 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3301 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3361 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3421 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3481 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3541 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3601 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3661 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3721 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3781 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3841 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3901 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3961 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     4021 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     4081 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4141 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4201 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4261 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4321 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4381 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4441 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4501 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4561 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4621 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4681 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4741 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4801 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4861 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4921 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4981 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     5041 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     5101 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5161 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5221 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5281 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5341 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5401 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5461 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5521 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5581 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5641 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5701 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5761 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5821 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5881 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5941 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     6001 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     6061 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6121 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6181 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6241 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6301 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6361 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6421 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6481 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6541 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6601 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6661 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6721 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6781 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6841 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6901 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6961 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     7021 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     7081 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     7141 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7201 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7261 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7321 tcgatgtcta ggaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
     7381 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
     7441 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
     7501 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
     7561 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
     7621 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
     7681 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
     7741 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
     7801 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
     7861 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
     7921 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
     7981 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
     8041 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
     8101 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
     8161 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
     8221 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
     8281 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
     8341 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
     8401 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
     8461 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
     8521 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
     8581 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
     8641 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
     8701 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
     8761 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
     8821 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
     8881 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
     8941 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
     9001 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
     9061 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
     9121 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
     9181 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
     9241 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
     9301 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
     9361 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
     9421 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
     9481 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
     9541 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
     9601 acaagttgct ctgtaaattt ttacacaact cttatttttt accaaagcac ttcaattgac
     9661 tttttctgtt attcccatta aattaaggtc tcatacatat cacttctatc ataaaaggga
     9721 gaataaaaat gaaacaaacc actaacttga ctgaagttca agacatactg caacagtcat
     9781 ccgttgccgt catttatctt tcgatgccta attgcagtgt ttgtcacgct gttcgtccac
     9841 gactagagga acttctcaca cattatgata ttccggcact tcatttggat gcatttgaaa
     9901 caccagaagt agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg atttttcatc
     9961 aaggaaaaga agtctttcgg caagcacggt tcatcgactt taaaaaaatc cgttatctac
    10021 tagatgaact gacagcagaa gatgattccc tttcttatga agaaatcttc cgaacaaaat
    10081 aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa agttttgcct tcatttgttc
    10141 aatcgctttt tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa
    10201 gaaatagatc aaggtttgca attcattcgt caaatcaaca ttttggatca atacatctga
    10261 cggagcattc aatcgagcag cggtaaagtt caagattcct ttgattcctg cttccgttaa
    10321 ctgattgact acttcttgtg ctctttcagc gggtatcgtt aaaattgcta cttcgatttg
    10381 ttggatcttg atttgttctg acatatcttc cattggataa acggggatac cgtctacgat
    10441 gcgcccaacg atttcctcat tcacatcaaa agcacaactg atacgtatgc tattactttg
    10501 atggaatttg tattttagta aagcacttcc caagttccct acaccgatca gcgcaacatt
    10561 tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt ctacatcata
    10621 accataccca cgttttccta attcgccaaa ataagaaaaa tctcgacgga tcgttgcact
    10681 atctacttga acggcttcac ttaattcagt tgaagacact tttttctttc cagcattatt
    10741 taaaatcctt aaatagcgat aatacaaagg cagtctttta gctgtcgcct ttggaataat
    10801 atgatctttc acgaaatgac ctccacgtat tgtctaatta catctttgat ttctccatca
    10861 tagcagtatt tataggaaaa aatcaattcc attgctcaga tattgtgaaa ttttattctt
    10921 tattcattta tttatcattt ctgcaaatct cgcagtataa tggaatttat ggtagactac
    10981 taattgaaaa atagtttttt atttgaggag aattcacaaa gtagaaaaga ggattccatg
    11041 atattattac aagccaatca gattgctcgc cattttggtg cagaagtctt atttgaaaac
    11101 atacatttag aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga
    11161 aaatcgactt tattaaagat cattgctgga attgaagcac ctgatgaagg aaagatcgct
    11221 aaaaataaaa cagcgactct cggatacctc gctcaggaca caggacttga ttccaatgaa
    11281 acagtttgga acgagatgct gaaagctttt gaacaagtta gacaaatgga agagcggatg
    11341 cgcgagctag agattttcat tagcgaaggt acgccagaca cacctgctta tagttcttat
    11401 ttaaaagaat acgaccggct gcaacacgat ttttcagatc gaaacggcta tggttatgaa
    11461 aatgaaattc gttccgtatt gcatgggttt aaattcgatt cttcatttta tgatcagaaa
    11521 atcgatactt tatcaggcgg acagcgcaca cgtttagctt tagctcgcat gcttttgcaa
    11581 caccctgaca ttttgattct agatgaacca acgaaccatt tagatatcga gacactttcc
    11641 tggcttgaga attatctgca aagctatact ggtgcattat tgattgtttc ccatgaccgt
    11701 tattttttag ataaagtagt caatgaagtc tatgaattaa gcagaagaaa aatgcgccat
    11761 tacaaaggta actattcgaa atatctagat ttgaaagcag aacagttagc aagtgaatgg
    11821 aaggcttatg aaaagcagca aacagaaatc cataaattag aagacttcgt tgctcggaac
    11881 cttgttcgcg cctctaccac aaaacgagcg caaagccgca gaaagacatt agaaaaaatg
    11941 gaccggctgg atcgtcctca aggggatgaa aaacaagcaa actttctatt cagtatagaa
    12001 aagccttctg gaaatgtagt cttgcaaatc gaagatgccg ctatcggata tgataatgtc
    12061 accttatctg aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc tctagtaggt
    12121 ccgaatggta tcgggaaatc gacgttgctg aaatcaatca ttcaccagat cccttttatc
    12181 cgtggaaaag aaacgctagg gacaaatgtc accatcggct attacgatca aggtcaagct
    12241 gatctgcata caaacaagac gatcctagca gaattgtggg atgagcatcc taccactcca
    12301 gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa
    12361 ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg
    12421 aacaaagaaa attttttgat tttggatgaa ccaacaaacc atttagatat tgacaacaaa
    12481 gaagtattag aaaatgcatt gatcgattat gacggcacac tgctttttgt ttcccatgac
    12541 cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa
    12601 gtctatctag gagactatga ttattattta gagaaaaagc aggaagaaga agaaatcgcc
    12661 gaattgttgg cacaagatga gccagaatct gctcctgctc ccacgaacaa atctgattat
    12721 taccaaagca aagaacagca aaagcttatc cgtagccttc aacgaaaaat tactcaggtt
    12781 gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg
    12841 ccagaaaacc tggaagatca cgtaaaactc aatgagctga accaagattt agaagctgct
    12901 agacaagatc aagaaacaaa attgacggaa tgggaagaac taagcttgga attagaagaa
    12961 tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg tttttctttt
    13021 tttattttat caaacaaaaa ttccatattt acctttttca ccaataagaa gaccaattac
    13081 tagtcattct cactaaagct tctaaataat agtaatcacc ccaaatacaa ggtgtattta
    13141 ccccattatt tttgggctta ttataaacag actgtgttaa aaagccatct tcttcttccg
    13201 ttgtagcata tctttctttc aattgtcgca taattttcca aacagcagac tggtataagt
    13261 tcttttctgt tttgtctaat agacctgatt ctattaattc taataatcca caaaccatga
    13321 tcaccatact agatgtatct ttttcttctc tgctagaatc atcaaagatc aaatcccaat
    13381 aacaaatggt atcttctggc aaccgattca taaaataatt ggctaatatt ttgcttaaat
    13441 caaaatattt ttgttctttc aaataacgat aacttaatgc aaaaccgtaa atcccccatg
    13501 cttggcctct agcccaacag gaattatcac tataaccttg atgcgtactt ccatatcttg
    13561 gttttccatc tttgacatcc atataaaatg tatgataagt tgaaccatct tctcttacta
    13621 aataatctgc tgcattttga atatgagcct gagcagcttt tttatatttt tgatttccag
    13681 tttcttcact cgcccaatac aataatggaa gattcatgca acaatccatg atcattctac
    13741 cttgttgtga aggatcacta ggatctcccc aagcttgtat aactcctgcc ttctcccaaa
    13801 aacgctgaag taatgtttct gcagcatcaa ttgaagcttc cttcattttt cgatttcccg
    13861 ttattttata atctgctacg gttgataaag aatacaaaaa accaagatcg tgatgattag
    13921 tgataatttg acagtcaatc ctgtggataa aatctatatc tgctttttca gctgtttgtt
    13981 gaaataaagg atcacccgtc ttctcaaata acaaccaatt aattcctgtc caaaatcctt
    14041 ctgtccaacc aatattgtct cttaatggat aacgtccttt tataggacac tcgcttggga
    14101 attgatactg actgttactg atacgtttga ttaacctctc cgtgtgtctg cgacaacgct
    14161 cgatctcatt attccattct ttttccatta attctttata acattcattt atccattttt
    14221 ctggatgtat tatctcttca aacatcactt tatcaagcat ttccccacca gcctttagca
    14281 taattttagt ctttttttat tgtttttatt ttggatagtt agcccggttg tcccatcaaa
    14341 atcaattttg ggacgattgt tccatacttc taaactataa tcccgatctt gatgagcaac
    14401 cactcctgtt ccgagccaat gggttccttt tccaaatgta ccctctaatc caggaataaa
    14461 agtgttttcc catgtcatca agtttgtatt gggcaaaccc ataatagctg tggattttcg
    14521 tttaatagta tcgtcactta aacagataat acctgaaaag ccagcttcac tcaccctata
    14581 gttttccttg tcttcttcta tgattattcc ttctttctca ggagaacatt caatagcaaa
    14641 ccctgtctca tatagaacat attctgtatc taattctact ttgtgtacgc gaatctgcca
    14701 gccttcaaaa ggaataagcc aagttctgat ttttgcctgt aaagtaggtt cccatgtaga
    14761 ggcaagtacc tctttcttac atacaatgtc tcgtacatta tttcgaacga tgaactgtcc
    14821 ataggttttg atgctctcat tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat
    14881 ccctagcatt gaatccattg ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa
    14941 accaaattga ctagaataag caaattttaa atatttttca ttggtcaatg tatggaaatt
    15001 tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt gtatgagcaa
    15061 attggcttct gaaagaagtt tagttccttc acgaataggc ataggcattt ctttagccgt
    15121 ccaatactca tggtcttcag gtagcgccaa caataaaaaa atcttattca gccataatgg
    15181 agataacgta ctattatagg gttcagtcaa tgtcaattga gggtaggtat aaccaatatt
    15241 caggatacct ttttcatcaa atatttcttt ttgcatccac catcttatat tccgattgac
    15301 taaccccttg atttcaccta atttaaaagg caaaagatcg gcccacaaca tagctgacca
    15361 aaagcttgaa actgcaaaac gataaatcat gcttcgacca aaaggcacat ttgcaccatc
    15421 ttccgcaaaa aaatgaatat agtctctaga aaaatcaata gctcggttct tatatatctg
    15481 gctaatgggc ttttctggtt ctagtacgct ataaatcaaa ccataataat gaaatgcaaa
    15541 aggattataa taatcttggc gacctcttga actgtctcga taccagccat tttgaagata
    15601 acaattttct atattatcaa atgcttcttt cagtgctgat tctttaactg gataaccaaa
    15661 tttttggata attgttccga caattatctt aaaaaattgc caattaccat ctgcacattc
    15721 atattcaaaa atttgcgaga accaatgaat aatctggctt ttttctcctg tatctaattt
    15781 tttccataat aattgttcat gatgatataa cgctaataac attgcgggca tttctaccat
    15841 acgctggtct ctttcatgta tttctcccca ataatcggga tgggaaggat ctgttccagc
    15901 ttttattcct gatagaatta atttcaacca tcgttcatct aattggtcaa ttgcgggccc
    15961 cgctccccat aaaacccgag agaatccttc cattcctgca attttatttc catatcctac
    16021 cccttgatgt atataatcaa tttgtgcgcc acttgctcga taaaacggct ggagtggttg
    16081 atttatcttt ttaaataagt caaagtaatc ctgtctggtc cttaatagtg gtactggatg
    16141 tttcattgta ttttctcctc tttttactac caaatagcta attccgactg ttctaatcga
    16201 agtaagcctt caataaaaaa ataatcagaa tagatcagtg gcacattata agtcagttct
    16261 ctatggtatg cttctgctcc gcctccaatg atcccatctt catccatatt ccaatttgca
    16321 tagtgctttt ctgtcttttg gagtatattg atagcagctt catgatacat ttttgatcgt
    16381 acagaggaaa catatcctgc taattccaga agaccgcatg cagcgcatac tccagcactg
    16441 gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag gaatataacc agttttttcg
    16501 attttctgaa taaaatttac tgctatcttt tctgctatag ctagatagta cggatttttt
    16561 gtgtaacgat aacttaacgc gaatccatat aaagcccatg catttcctct gctccaacaa
    16621 gaattctcat tgtatccttg cccgccaagt aaccgaatat atttccctga ttctgcatca
    16681 aattctgcga tatgcccggt tgatccatct ttcctcacta gatacttagc tgttgtatct
    16741 gcatgctgcc gagcaatttt agagaacctc ggatcaccag tttccctcga agcccaatac
    16801 agtaatggta aattcatcat actatcaata atcatccaac caggtttatg gtcattccat
    16861 gctactaaat agttccctga aatattatag cgtccagcaa gtaccgtagc tgcatgtaac
    16921 cctagttgtc gactatgttt gttttgagac tgttgataat gtgcaatagc cgatggtaag
    16981 aataaaaaac ctacatcatg atgaacgtct gaaaaatggt acagggcaga ttctaataat
    17041 tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc
    17101 cataaaatac cactccaaaa accattggtc caccagtcga ttcctctgtc ctttattacg
    17161 tctgtatatt ttccgttgta agaaatatat ggaattttat tccccattct gtctaattcc
    17221 gcatctatct tttcagaagc tacattcaaa atttttcttg accattcctt cgtctgttca
    17281 ttcatggata tcccgccatt cttttaattt ttatgaatgt ggagttgctc ctagaacgaa
    17341 ctagatggtt aacattcgtt atttttttta cttatttgca gctccaatgt cattctcctt
    17401 aaatttagac tgcgcccaca cataaatcaa gaccattggg atcactgaag ttgctaaaat
    17461 tagcataatt gcgccataat taattggctc acctgtacta gaaaccgtca tatttttgat
    17521 aattaaggga accgtatatt tatctttatc tgaaagcatg actaatggaa atagatattg
    17581 attccaactt cccataaaga taagtaaacc actagtaata atcgtaggtg ataaaatcgg
    17641 tacagcgatt ttcgtaaaaa tcacccactc attggcacca tcgattctgg cagcctccat
    17701 cagtgctgta ggaaattgac tgctatattg tcgcatcata aacactgaag ttgtactggc
    17761 aatcgaaggg atgatcacac ctgcataagt attcgataat cctaaacgac tcataatgta
    17821 aaataaagga atcaatgtgg taaaaaaagg caccatccta gatagcataa ctgtattgaa
    17881 caacagttta ttccccataa aacgatactt tgctaatgca taacccgcca ttgtagaact
    17941 ggctgtagct aaaactgtac tgattacagc aataacaatc gaattccaaa taatcaaggg
    18001 ataattatat ctcgccatga tggcttgcca gttttttaat agcatatccc caaacttgaa
    18061 attgattccg ccacttaaaa tttcttgatt actttgcgtt gaagctacaa tcatatataa
    18121 atatggcatc aaaaatatta aggcaccaaa cacaataatg acgtacgtaa aaattctgct
    18181 ttgtatcttt tgtttagtca tttttatcac ctccgaattt aaattgaata gccgataata
    18241 taacagatac aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt
    18301 caaaacttgt tttatataaa ttgactccta cgaccaaagc tgaattttct ggtccattgg
    18361 aagtcccaaa gaatatattt gggatattga aattacccaa gccattgata gtggctaaaa
    18421 ctgtggccat taaaatgatt ggctttagca tggggatcgt taataaaaag aattgccgaa
    18481 atttcgatgc tccatctatt tcagcacttt cgtatatctc tttagaaatc ccttgaagac
    18541 cagctaaaaa taaaatagtg ttatatccta cactaagcca aatcatcgtg atgatcattg
    18601 atattcgtat ccaaaaagga tttgttaacc actgtacacc gttaattcct agcgcattca
    18661 aaaatccatt gaataaccca gtcggattga aaaaagtcat gaatacggaa gctacagcca
    18721 ccgttgatgt aatggttgga atataataga cagttctaaa aaatcctttt ccttttaact
    18781 gtgctgaatt caataaaaca gcaaagtaaa tagctaatag catcgttatt ggaattgatc
    18841 ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa caatggatct cggaagatag
    18901 ctagataatt gtcaaatcca acaaattcaa atgagtagcc aattttcata aaactaaaag
    18961 caaacgagat aatgatggga atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga
    19021 ccaatccata agcacttttt ttatgagacg tatctgttgc ttttttcttg ctgttcttta
    19081 ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc ctcctaatat tttttaatca
    19141 ccagttaatt ttacgccgta cttatgagca aaattttctg cttcttttag atatgaattt
    19201 tcaaaatcac ctgtttgcca atagttatat gcagcggtag tcaaataaga cgtagcatct
    19261 gaataagtgg ctggtaaaac agtatttgat gtattttcat tgattttatt caatagttca
    19321 tagtattttt gtcctcctta gtagtcaaac tttttctcag ctgcttctgt ttgataggcc
    19381 aatgagttag atgtagccac gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc
    19441 agcataaatg taagaaattg ttgtgccact tgtggatttt tggcatcttt agggacataa
    19501 aaacttgatc ctcctgatac tgcacctttt cctggatttt cttttgaaaa aggaatcagt
    19561 ggagccatgc gccatttgtc agatgcatca ggaaaattca aagacatatt cgtagcatac
    19621 cagccgccag caacaaacat tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg
    19681 tagaaatgta caatatctga gtcgatcatt tctttgatta actttgcagc atgtttcgct
    19741 tctttagtat tcagacggat atttccttct tcatcaagca aagattgatt ctgctgggct
    19801 agcatgttga taaacaattc ttggtcccca gattctggca aggcaattcc aaaactacca
    19861 gttttttctt ttaatgcttt tgccacctgg atatattctt cccaagaact gatttcttca
    19921 aaatctacac ctatttcagc aaattttttt ggttgataat aacaaagtgc tggggtaaaa
    19981 tcactagccc aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt
    20041 tgatcataaa actgatcgcc atatttgtca taaaatccat attcatcagc actataaaaa
    20101 gcatcttgaa atttttccag cacacctgct gctgaagtgt ctgccatgga gataatatcc
    20161 ggcatatctt cgtgtgttac caattttggg gtgatcattt gtactgttgc tgttcccgaa
    20221 gctacattca caatttttaa gtcaatattt gctccagttt tgcggttata gatttttgca
    20281 gcattttcaa catagtttgt cagtttgccg tttgcgacat atacttctaa tgtagtggct
    20341 ttttttatca ttggatctgc gatgtttgct gttacttctt cgttagtttt tccacatgca
    20401 cttaagctga ataatagggt aatgtttagc aaagctatca ccaatttgta tctttttttc
    20461 atgtctatcc cttctttctt ttcaagttct tatataaaac gctttcactt atgtatatta
    20521 acggaattag taacacaaat atttatataa aaaaatattt tacttgtcgt tttggctttt
    20581 catttttgtt ataatagtca ttaattataa atagaaagct ttttatataa aaagagaaag
    20641 gaaagtggtt cttttgttaa caaacaacca atcctccaat cttgtcaaaa aaacactaac
    20701 ctattccata gatacccatc ttcatttatc tgctgctccc tattttcgac tactaataaa
    20761 tgaaagcgct aatttatatg ctgaatttgc acataaatct atagaactag ataatccttc
    20821 ccttttattc attgataaac agcaagaagt ccaaatcttt tctactcaaa aggaaacaca
    20881 aacttttatc attatccagt tttattttga tgagcaatta ttagcacata ttacgggaca
    20941 atctttttct atagatcaag aaataacatc tatcattact tttctgaaaa atgaacagtt
    21001 caaaattgat tatctaaaac atagtaagcc agctatttcc tatgatcagg aaatcaattc
    21061 ttttttcata ctatgccaac agatgagcat tcatttgctt tccttattag ttacttattg
    21121 tctgcaaaaa attcaattgt cttccttacc aaggcattat caacaggaac ttgctttatt
    21181 gacaatacct tcaaatgaga aagtcattcc taaaaaaaag catattcttt attcagatga
    21241 attagttgat ccaatcatac gatatttaga agaacatctt tcagaaaatt ttttgcttga
    21301 acaattagcc caaatttttt ttatcagtcc agccagctta aaaaaacatt ttagaaaagt
    21361 gaatggaaaa agtgttatta gctattttaa agaactaaaa atggaaaaag caaaacgtct
    21421 cgtgctagaa aatgaattaa cctatacaga aattgcagat atattaggat ttcattcgat
    21481 ccatcatttt tcttcagctt ttaaaaaata tacagggtta tccccttcaa agtattatct
    21541 atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct
    21601 gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca
    21661 tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta
    21721 gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt
    21781 atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga
    21841 ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa
    21901 aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga
    21961 ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc
    22021 atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac
    22081 ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa
    22141 aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat
    22201 ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct
    22261 tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt
    22321 catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat
    22381 ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa
    22441 gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag
    22501 ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta
    22561 gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg
    22621 ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc
    22681 gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca
    22741 tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat
    22801 ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta
    22861 ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca
    22921 atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg
    22981 tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg
    23041 aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc
    23101 tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat
    23161 attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac
    23221 attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt
    23281 atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg
    23341 agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta
    23401 tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta
    23461 tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa
    23521 aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac
    23581 gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct
    23641 ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg
    23701 actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa
    23761 tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg
    23821 tattaaataa tttaattttt ttgttcctat ttatttaa
//
LOCUS       DAFFQY010000040        23645 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFFQY010000040 DAFFQY010000000
VERSION     DAFFQY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.040"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            248..523
                     /locus_tag="LRB02_001477"
     CDS             248..523
                     /locus_tag="LRB02_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659681.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="LRB02_001478"
     CDS             536..2614
                     /locus_tag="LRB02_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL4659682.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="LRB02_001479"
     CDS             2774..4660
                     /locus_tag="LRB02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4659683.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="LRB02_001480"
     CDS             4672..5619
                     /locus_tag="LRB02_001480"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL4659684.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="LRB02_001481"
     CDS             5639..6166
                     /locus_tag="LRB02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4659685.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="LRB02_001482"
     CDS             complement(6229..6882)
                     /locus_tag="LRB02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL4659686.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="LRB02_001483"
     CDS             7014..7856
                     /locus_tag="LRB02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4659687.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8909
                     /locus_tag="LRB02_001484"
                     /pseudo
     CDS             8014..8909
                     /locus_tag="LRB02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
     gene            8912..9625
                     /locus_tag="LRB02_001485"
     CDS             8912..9625
                     /locus_tag="LRB02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL4659688.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9645..10163
                     /gene="msrA"
                     /locus_tag="LRB02_001486"
     CDS             9645..10163
                     /gene="msrA"
                     /locus_tag="LRB02_001486"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4659689.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10160..10387
                     /locus_tag="LRB02_001487"
     CDS             10160..10387
                     /locus_tag="LRB02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL4659690.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10539..11090
                     /gene="lepB"
                     /locus_tag="LRB02_001488"
     CDS             10539..11090
                     /gene="lepB"
                     /locus_tag="LRB02_001488"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL4659691.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11173..11673)
                     /locus_tag="LRB02_001489"
     CDS             complement(11173..11673)
                     /locus_tag="LRB02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL4659692.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11952..12350)
                     /locus_tag="LRB02_001490"
     CDS             complement(11952..12350)
                     /locus_tag="LRB02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4659693.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12610..13470
                     /gene="ylqF"
                     /locus_tag="LRB02_001491"
     CDS             12610..13470
                     /gene="ylqF"
                     /locus_tag="LRB02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL4659694.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13463..14230
                     /locus_tag="LRB02_001492"
     CDS             13463..14230
                     /locus_tag="LRB02_001492"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL4659695.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14286..15143
                     /gene="dprA"
                     /locus_tag="LRB02_001493"
     CDS             14286..15143
                     /gene="dprA"
                     /locus_tag="LRB02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL4659696.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15265..17343
                     /gene="topA"
                     /locus_tag="LRB02_001494"
     CDS             15265..17343
                     /gene="topA"
                     /locus_tag="LRB02_001494"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL4659697.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17312..18688
                     /gene="trmFO"
                     /locus_tag="LRB02_001495"
     CDS             17312..18688
                     /gene="trmFO"
                     /locus_tag="LRB02_001495"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL4659698.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18886..19791
                     /gene="xerC"
                     /locus_tag="LRB02_001496"
     CDS             18886..19791
                     /gene="xerC"
                     /locus_tag="LRB02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL4659699.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19828..20376
                     /gene="hslV"
                     /locus_tag="LRB02_001497"
     CDS             19828..20376
                     /gene="hslV"
                     /locus_tag="LRB02_001497"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL4659700.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20390..21790
                     /gene="hslU"
                     /locus_tag="LRB02_001498"
     CDS             20390..21790
                     /gene="hslU"
                     /locus_tag="LRB02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL4659701.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21808..22599
                     /gene="codY"
                     /locus_tag="LRB02_001499"
     CDS             21808..22599
                     /gene="codY"
                     /locus_tag="LRB02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL4659702.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22681..23556
                     /locus_tag="LRB02_001500"
     CDS             22681..23556
                     /locus_tag="LRB02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL4659703.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8189 a         4078 c         4982 g         6396 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaatgccg aactgctact ttatcccaat caatgatttg ctttatcaag
     8701 gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact agcacgacag
     8761 aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc catccaaata
     8821 atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag acacaaaacc
     8881 aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg tatcttcaaa
     8941 caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg gggtagtact
     9001 tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag aactcccaga
     9061 aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt caaataaaga
     9121 aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata gtgatattga
     9181 atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag tactaggttt
     9241 tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa atgtccaact
     9301 aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg tgatgaatat
     9361 gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg atctgactat
     9421 catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag cagataaaat
     9481 cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata cctctgatgc
     9541 ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca agacaaaaga
     9601 aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa cgagcaatat
     9661 ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct ggtatccata
     9721 cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa caagtaaaaa
     9781 gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca ttgatttcat
     9841 atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct ttcggacaat
     9901 tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa gaacagcgga
     9961 tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa cagccaatcg
    10021 ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat caagagtatt
    10081 ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc tttatcgaag
    10141 aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga cactgcgagg
    10201 cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag acatccaatt
    10261 tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga atgtcgacta
    10321 tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa ataatcatga
    10381 catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa tagcctaatt
    10441 gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt tcttataata
    10501 aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa caacgctata
    10561 ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt gcgttcatgt
    10621 tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa aacgtgttga
    10681 agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt gatattgttg
    10741 tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt ccaggagaaa
    10801 atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag gaaccttatc
    10861 taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac tttacactac
    10921 aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt ggagataatc
    10981 gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat attctaggta
    11041 aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga gaaacaataa
    11101 agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag tttactacct
    11161 tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag ttttgataaa
    11221 tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga atctacctag
    11281 aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat tgataaaaag
    11341 gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt ttatcttttt
    11401 atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa gaaaagcagc
    11461 ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca tttcaagcaa
    11521 accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa atccgaatgt
    11581 gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa gggctaataa
    11641 aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa cgtcttttgc
    11701 aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga taccataaaa
    11761 tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac aattaaaaaa
    11821 agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata aaaaaaatag
    11881 aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa aacgtcagat
    11941 tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc tagatcttgg
    12001 acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg gaaagtacca
    12061 ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc aacactaaac
    12121 gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt tataaatttt
    12181 ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac cattgaacca
    12241 gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata aaaagcaacg
    12301 gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat ttgtttcgtc
    12361 tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa aaatttagat
    12421 tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat gtgatagaat
    12481 gaggactggt tcactttcac ttaactaata gatggaattt ctttataata aaaaagtgaa
    12541 tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat gaaaggaatg
    12601 gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg acgagaagtt
    12661 tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg attaccttta
    12721 tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct tgttttattg
    12781 aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata ttttcgtaaa
    12841 caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa aggtctagta
    12901 cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg ggggatcaag
    12961 cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc tacgttgatg
    13021 aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt aacaaaaggt
    13081 cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg gattttatgg
    13141 cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc gatcaaagac
    13201 cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc ccgatattat
    13261 tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa attggcaccg
    13321 gaattactga tggatattac agcaaaacgt gggtataaag aagactatga tcgtggcagc
    13381 gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact ggatcgttgc
    13441 gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag aacgattgaa
    13501 aagcatacag agaatagatg atcctacact agagtcatta agaaatgatc cacgcaaagg
    13561 cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat taattaagga
    13621 aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc accaatggat
    13681 tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg cagctgcagt
    13741 tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat tatcagagaa
    13801 aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg ggatcgggat
    13861 cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat tagcgatgat
    13921 ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg cgatgacctt
    13981 ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg tgtcgattgc
    14041 tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat atgacgtgct
    14101 ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac acttagaagg
    14161 acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg taaaaaaata
    14221 tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg aaaggcggtt
    14281 ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa ggaatcagta
    14341 gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa gaaatcggat
    14401 ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt cgcaaaagtt
    14461 ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt atcacttgct
    14521 ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt gtactgtttt
    14581 ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt ggaagacaca
    14641 cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata gaagctggat
    14701 atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa gcagcaatct
    14761 ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc tatcctgctg
    14821 aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt gaatatcctc
    14881 ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc gcagctttgt
    14941 ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata acagcacagc
    15001 aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta acagaacatt
    15061 ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg ggtgaagata
    15121 tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg acaaacgttt
    15181 tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt aggaacacga
    15241 caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt agaatcacct
    15301 gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat ggccagtgtt
    15361 ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa caattatgaa
    15421 ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa agccgcagca
    15481 aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga agcgattgct
    15541 tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt ggtcttcaat
    15601 gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat cgatgtagat
    15661 ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta ttcgatcagt
    15721 cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca atctgtcgct
    15781 ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga agaatattgg
    15841 agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt ctggggaatc
    15901 gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac tgctagaatt
    15961 gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag aaaccctgcg
    16021 aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa ctttcgaaca
    16081 agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg taaacaagga
    16141 acagtcggct tgattacata catgcgtact gattctaccc ggattgccga tacagcaaaa
    16201 gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag acatgatcac
    16261 cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg tccaacgagt
    16321 gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt gaaactttat
    16381 acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt agatacaatg
    16441 aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa aatcaaattc
    16501 aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga gaaagaaaat
    16561 attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga accaaaacaa
    16621 cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc actagaagaa
    16681 aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca aagacgctac
    16741 tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat tgtcaattca
    16801 ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga aatggaagga
    16861 gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga tcgtttctat
    16921 aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat tcaaatcaaa
    16981 gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat aaaattaggg
    17041 aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac gaaaccaatc
    17101 gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat tgaacgaaaa
    17161 tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga attcacttct
    17221 tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt cgagaagaaa
    17281 gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga catccaaaaa
    17341 taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag ggcttgccgg
    17401 aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat atgaaatgcg
    17461 tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag tatgttccaa
    17521 ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag aaatgcgccg
    17581 attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg gtggtgcctt
    17641 agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag agcatccatt
    17701 agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg tagtagcaac
    17761 aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg gttcagatgg
    17821 tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg atatggataa
    17881 agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact gtccgatgac
    17941 agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg cagaacttaa
    18001 atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa tggcacagcg
    18061 aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag atcctaaaac
    18121 tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag cttctttata
    18181 caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg ttttccggat
    18241 gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc gaaacagctt
    18301 tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg aagatctgtt
    18361 ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga gcggactact
    18421 tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt ttccacaaga
    18481 aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac atttccaacc
    18541 gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag acaaaaaagc
    18601 aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa aaacagaatt
    18661 agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga gacggatagt
    18721 ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga ggaattgcgc
    18781 caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg tctttttata
    18841 aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac agaagccacg
    18901 tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga aaaaacgaaa
    18961 gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc tggagacgct
    19021 gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt agctgatcat
    19081 cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg ttcgttttat
    19141 cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt tcatctcaaa
    19201 aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc actttttgac
    19261 agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga agtcctttac
    19321 gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat tgattttgat
    19381 tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc tctaggttct
    19441 tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt gatgacaaaa
    19501 tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat cacgcctact
    19561 ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag tgatatccat
    19621 cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc agatttgcgg
    19681 acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta tgcccatgta
    19741 acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta aaaataagga
    19801 aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc aaccactatc
    19861 tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt aacaatgggt
    19921 gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa tggagaagtt
    19981 gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa atttgaaggc
    20041 aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc tcaagaatgg
    20101 cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa tgataaagaa
    20161 atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat cttagctatc
    20221 ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta tgctcaaaac
    20281 gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga tatctgtatc
    20341 tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata tgtatgaatt
    20401 aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag gacagcacgc
    20461 agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc agttagatga
    20521 aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta caggggtcgg
    20581 taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg tcaaagtcga
    20641 agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa tggttcggga
    20701 tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg tatacacaag
    20761 tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta tcaaaaaaga
    20821 acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg ctagtcagca
    20881 aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc gccgtacagt
    20941 cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg aagttgaaga
    21001 ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga tcgacctaaa
    21061 tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa cagtcaaaga
    21121 agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg ctgatatcca
    21181 tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg aaatcgataa
    21241 gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc aaagagacat
    21301 attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc aaacagatca
    21361 cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc taattccaga
    21421 acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg aagattttgt
    21481 gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt tgatcggtac
    21541 agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc aaatcgcttt
    21601 cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca ttttggaaaa
    21661 actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa taacgatcac
    21721 agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt taagtcgcta
    21781 tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac ccgcaagata
    21841 aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc ttacgataaa
    21901 atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg aagtgacgga
    21961 gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa aacaatgttt
    22021 gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat gaaaactgaa
    22081 gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag agagaaatat
    22141 ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt gggtacgatt
    22201 atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc agaatatgga
    22261 gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga agcagatgta
    22321 cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga attgaaggct
    22381 gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc ttctaatatt
    22441 gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa attggaatca
    22501 gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa agtattgaat
    22561 tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga ctggggcaga
    22621 tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg aggaatggca
    22681 atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca cggtgcggaa
    22741 ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg tgatccagcg
    22801 tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa agatgatcag
    22861 tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg ggatatggat
    22921 tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag tacgccggag
    22981 acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt aggaggagac
    23041 ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt cttttctatc
    23101 ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga agatttctat
    23161 ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc attcatcgat
    23221 atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg tgaacttttt
    23281 gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat tcgaagtgaa
    23341 aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg gatctggtct
    23401 ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat cgctgacacg
    23461 gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg tgcccatgaa
    23521 atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga gaagttagca
    23581 gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa gggagtgtga
    23641 catcg
//
LOCUS       DAFFQY010000041        22963 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFFQY010000041 DAFFQY010000000
VERSION     DAFFQY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.041"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..783
                     /locus_tag="LRB02_001501"
     CDS             <1..783
                     /locus_tag="LRB02_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL4659704.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="LRB02_001502"
     CDS             1161..3203
                     /locus_tag="LRB02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4659705.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="LRB02_001503"
     CDS             3214..3834
                     /locus_tag="LRB02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL4659706.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="LRB02_001504"
     CDS             3846..4304
                     /locus_tag="LRB02_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4659707.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="LRB02_001505"
     CDS             4323..4601
                     /locus_tag="LRB02_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4659708.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="LRB02_001506"
     CDS             4626..5993
                     /locus_tag="LRB02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL4659709.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="LRB02_001507"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="LRB02_001507"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBL4659710.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="LRB02_001508"
     CDS             7180..7671
                     /locus_tag="LRB02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659711.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="LRB02_001509"
     CDS             8114..9313
                     /locus_tag="LRB02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4659712.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="LRB02_001510"
     CDS             complement(9808..10566)
                     /locus_tag="LRB02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4659713.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="LRB02_001511"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="LRB02_001511"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL4659714.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="LRB02_001512"
     CDS             11666..13111
                     /locus_tag="LRB02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4659715.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="LRB02_001513"
     CDS             13139..13594
                     /locus_tag="LRB02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4659716.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="LRB02_001514"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="LRB02_001514"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL4659717.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="LRB02_001515"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="LRB02_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="LRB02_001516"
     CDS             15403..16017
                     /locus_tag="LRB02_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659718.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            16258..16641
                     /locus_tag="LRB02_001517"
     CDS             16258..16641
                     /locus_tag="LRB02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4659719.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            17004..17513
                     /locus_tag="LRB02_001518"
     CDS             17004..17513
                     /locus_tag="LRB02_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL4659720.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17613..18263
                     /locus_tag="LRB02_001519"
     CDS             17613..18263
                     /locus_tag="LRB02_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659721.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18714..19652
                     /locus_tag="LRB02_001520"
     CDS             18714..19652
                     /locus_tag="LRB02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL4659722.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19665..20717
                     /locus_tag="LRB02_001521"
     CDS             19665..20717
                     /locus_tag="LRB02_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659723.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20983..21617
                     /locus_tag="LRB02_001522"
                     /pseudo
     CDS             20983..21617
                     /locus_tag="LRB02_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
     gene            21808..22599
                     /gene="msrA"
                     /locus_tag="LRB02_001523"
     CDS             21808..22599
                     /gene="msrA"
                     /locus_tag="LRB02_001523"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4659724.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7303 a         3682 c         4842 g         7136 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt
    16201 acaaaaattt gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg
    16261 ccaaaatata aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag
    16321 gaaggttcta aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt
    16381 actatccgca aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga
    16441 tctggtactt ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat
    16501 aatgtatttg gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt
    16561 gatgtccggt ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct
    16621 tcctgcaaat catgggtata aaaaataggc atattaagcg ggctaggagg agaaatattc
    16681 acatatcatt ttttcattcc gcaagctgcg tcaagttatt tggaatttgt agaacagatg
    16741 agagaggtag aagcagcact acaaactact tttcaataag taggaattat agaaatttta
    16801 ctcaaaaaat cttcattaaa aaatctgact aaataagttg agaaaacaga atatacttca
    16861 atatccagtg atttataaat atcattcact catgataccc agctccgtat cataagtgat
    16921 gcggttttta tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa
    16981 gactaaatac ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg
    17041 cgattgattt gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga
    17101 ctaaagaaga agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag
    17161 aaaataatgt gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc
    17221 tgattctctt ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa
    17281 gcaaggaaat caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta
    17341 tgaccactga tgatatttta gaagtttttg ctgaggcaga actctatcca tcaaaaggcg
    17401 aaatcggggc atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat
    17461 atatgcgtaa ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt
    17521 tcatgagaaa tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa
    17581 tattatctta atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag
    17641 ataagatgct ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa
    17701 agatgtcttt aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac
    17761 atgttgaaaa attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac
    17821 agccgattaa atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag
    17881 atcaatatat tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat
    17941 acaattattt gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt
    18001 cttctaatga tattttttta gatggagatc ttttttatca actcaataca aatcacttgt
    18061 ctggcactga aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg
    18121 atgagatttt aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg
    18181 ctaagaaact ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa
    18241 attattattc ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta
    18301 aaaatggaag aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa
    18361 ataataagca ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc
    18421 acaaaaagaa aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc
    18481 ccctctttta taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc
    18541 tagtctagtt tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga
    18601 taaaataact tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg
    18661 ttgtgaaatg attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa
    18721 tagcaattgc aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg
    18781 ggaacgaagt attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg
    18841 gattacaagc aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat
    18901 ccgaagtaga aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc
    18961 tggaaaaaat gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc
    19021 tattaaatgg tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct
    19081 atattggaaa taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg
    19141 gaagtggttc agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat
    19201 tagcagacaa attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt
    19261 ctatttatcg caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg
    19321 ttaatatggc tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca
    19381 atgagtttgc caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg
    19441 cacattgtga atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc
    19501 aagacttgat taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta
    19561 gaaaagggaa gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc
    19621 atgcaaaaga agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa
    19681 aaatatcacc gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta
    19741 ttggattgat tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct
    19801 ttgcggtaac gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg
    19861 gtggtttgat tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag
    19921 cttcttttgt tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt
    19981 acgttggtgc tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg
    20041 ggctgatttt actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg
    20101 ttgtaggtgt aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta
    20161 cttctgcaat tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta
    20221 tcttgattgg ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg
    20281 gaatggcaat ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa
    20341 ccacttttgt tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga
    20401 ttgctttagg cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta
    20461 ttccttgttt agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag
    20521 gtacgcctgc ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag
    20581 atgcagggct aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg
    20641 cactaattac tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct
    20701 tcaaattttt aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga
    20761 gatgtcaaca acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac
    20821 aactgaactc ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt
    20881 attgattgtt caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat
    20941 tgttggatgt atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa
    21001 tatggttttt attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt
    21061 ggcctttttt atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta
    21121 gatagtccag tcagacgtgc tttcttggtc tggtcagttg tatctggact ttttttgttc
    21181 tttcgttgcc tgcgttgtat cacctattta ttggaacatc aaaaacactt gcaatacttg
    21241 ctgttgcaac agttggttta tatgggatag gtgattgtat ttttactggg ttattcagta
    21301 tcaatacgaa tgaaagctct tggaatctat cgacttggat ccataatata ggttctgggc
    21361 taggctatgc aggctttcta cttttcccat tgttacttgt tctgctttat cgtcaaagtg
    21421 gtcaaggtac gcttagccat ttttatcttg ttttaacagt tattagttta ctaattgcag
    21481 gtctttacgg attagcacgg attccgtcga ttagtcagtt tgccttcttt aagcaattgg
    21541 gcttctttca acgcttaagt tttttcttta actatttgcc gatgatttgt ttaagttttt
    21601 ttcaactacg aaagtagaaa aagcagtgtg aagctgatta agtaaaagaa tttctctatg
    21661 ggtaaccatt cgtgtggatg tgttcgacta agaatagaga tgaggagagc gaaaagcaag
    21721 ttagtcttaa cttgttatgc gttgtgctaa atttcaggta gtcatttttt gaatgaataa
    21781 gaagaaaact tgaaagaggg tgacttcatg ttagatgtgg agcagatgga aatagacctt
    21841 tataatatta ttttaaatcg aaatacaaga gaatgggaaa gaacacaatt tgaagaggca
    21901 aaagaagacc tagcaaaaat gtcgataaag caggtgatag aaaaactaga ggtcaacttg
    21961 cgtccgctag cactacgaga taatttgaca ccagatgcga tggattttta tttaagaatc
    22021 acaggggagt cagcgagtgc aaaggcttat gattatgcca aacataagat aacttcaccc
    22081 ttataccaag aacgggcgat ttttgccggt ggctgtttct ggtgtatggt tgagcctttt
    22141 gaaacaaaac caggtgtaat ctcagtgtta tctggttata caggtggaga gaccaaaaaa
    22201 ccaaattacg atcaagtcag tggtggatat actgggcatg ttgaagctgt tgagatcata
    22261 tttgatacac gtcaaataac ctatgaagag ctgttggaga tttattggca aatttcagac
    22321 cctacagatg cctttggtca atttcaagat cgggggaaac aatatcgttc aattctattt
    22381 gttgttaatg aaaagcaaag gcaattggct gaaaaatcaa gagaacaact gattcaatct
    22441 aaccgattta gccgtcctat cgttacagag attcgccaag caaagacttt ttggccagct
    22501 gaaaatcatc atcaacaatt ttatcagaag caacctaaaa gatataaaaa gatcaagcat
    22561 tcgcatcagc ttcatttatt ttttcagcga catagataga ggaaggttgt ggcgcaaagc
    22621 gttcagctcc gacaaaataa tgagcaactg acaaaaatag cttttcatat ttttggacag
    22681 ttatgaatta tttcgaggag ttgctttgag aacaccgagg aaaaggttgt ggcgcaaagc
    22741 attcagcttc aagtcgcaag gaaacaggag gtatgacaag tcttttgtca caactcctgt
    22801 ttttttacta gcctttattt ccatgtgaaa gagaattcga attgtagaat cttgattttt
    22861 ctatggaatt ttgtagagaa tcatcttgat aattaataga cacctaatcg aattccacat
    22921 taatgatgaa ctaaattccg tataaaagcg caaacaataa cta
//
LOCUS       DAFFQY010000042        22959 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFFQY010000042 DAFFQY010000000
VERSION     DAFFQY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.042"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LRB02_001524"
     CDS             4..816
                     /locus_tag="LRB02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL4659725.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LRB02_001525"
     CDS             813..1613
                     /locus_tag="LRB02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL4659726.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LRB02_001526"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LRB02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL4659727.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LRB02_001527"
     CDS             3110..4819
                     /locus_tag="LRB02_001527"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL4659728.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LRB02_001528"
     CDS             5031..9383
                     /locus_tag="LRB02_001528"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL4659729.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LRB02_001529"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LRB02_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL4659730.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LRB02_001530"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LRB02_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL4659731.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQVQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LRB02_001531"
     CDS             11221..11514
                     /locus_tag="LRB02_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL4659732.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LRB02_001532"
     CDS             11511..11822
                     /locus_tag="LRB02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL4659733.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LRB02_001533"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LRB02_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL4659734.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LRB02_001534"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LRB02_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL4659735.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LRB02_001535"
     CDS             complement(14623..14844)
                     /locus_tag="LRB02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4659736.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="LRB02_001536"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="LRB02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="LRB02_001537"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="LRB02_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL4659737.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="LRB02_001538"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="LRB02_001538"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL4659738.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="LRB02_001539"
     CDS             20242..21027
                     /locus_tag="LRB02_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4659739.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="LRB02_001540"
     CDS             21040..21834
                     /locus_tag="LRB02_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL4659740.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="LRB02_001541"
     CDS             complement(22014..22466)
                     /locus_tag="LRB02_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659741.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7799 a         3993 c         5003 g         6164 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagttca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gaccttttt
//
LOCUS       DAFFQY010000043        22023 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFFQY010000043 DAFFQY010000000
VERSION     DAFFQY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.043"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(59..1369)
                     /locus_tag="LRB02_001542"
     CDS             complement(59..1369)
                     /locus_tag="LRB02_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL4659742.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="LRB02_001543"
     CDS             complement(1381..2799)
                     /locus_tag="LRB02_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4659743.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3039..4607)
                     /locus_tag="LRB02_001544"
     CDS             complement(3039..4607)
                     /locus_tag="LRB02_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL4659744.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDLSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4611..4892)
                     /locus_tag="LRB02_001545"
     CDS             complement(4611..4892)
                     /locus_tag="LRB02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL4659745.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4889..5812)
                     /locus_tag="LRB02_001546"
     CDS             complement(4889..5812)
                     /locus_tag="LRB02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL4659746.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5809..6687)
                     /locus_tag="LRB02_001547"
     CDS             complement(5809..6687)
                     /locus_tag="LRB02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL4659747.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6674..8011)
                     /locus_tag="LRB02_001548"
     CDS             complement(6674..8011)
                     /locus_tag="LRB02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL4659748.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8014..9117)
                     /locus_tag="LRB02_001549"
     CDS             complement(8014..9117)
                     /locus_tag="LRB02_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4659749.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9191..10699)
                     /locus_tag="LRB02_001550"
     CDS             complement(9191..10699)
                     /locus_tag="LRB02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4659750.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10665..11357)
                     /locus_tag="LRB02_001551"
     CDS             complement(10665..11357)
                     /locus_tag="LRB02_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4659751.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11379..12353)
                     /locus_tag="LRB02_001552"
     CDS             complement(11379..12353)
                     /locus_tag="LRB02_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4659752.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12653..13524
                     /locus_tag="LRB02_001553"
                     /pseudo
     CDS             12653..13524
                     /locus_tag="LRB02_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(14106..16304)
                     /locus_tag="LRB02_001554"
     CDS             complement(14106..16304)
                     /locus_tag="LRB02_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659753.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16536..17528
                     /locus_tag="LRB02_001555"
     CDS             16536..17528
                     /locus_tag="LRB02_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659754.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17588..19009
                     /locus_tag="LRB02_001556"
     CDS             17588..19009
                     /locus_tag="LRB02_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL4659755.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19002..20501
                     /locus_tag="LRB02_001557"
     CDS             19002..20501
                     /locus_tag="LRB02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL4659756.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20540..21934
                     /gene="uxaC"
                     /locus_tag="LRB02_001558"
     CDS             20540..21934
                     /gene="uxaC"
                     /locus_tag="LRB02_001558"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL4659757.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3820 g         7040 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     2941 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3001 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3061 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     3361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     3421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     3481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     3541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     3601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     3661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     3721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     3781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     3841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     3901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     3961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4201 tcaagatcat ggaacaaaga aagatcaaag ccgttactat ttaaataaat tggttcggac
     4261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     4381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     4441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     4501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     4561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     4621 tttctcattc ttactcgata taaactgaat gccccagctg ctaaaaaagc aagtgaaata
     4681 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4741 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     4801 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     4861 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     4921 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     4981 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5041 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5101 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5161 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5221 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5281 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5341 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5401 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5461 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5521 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5581 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5641 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5701 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     5761 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     5821 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     5881 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     5941 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6001 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6061 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtgtttcgat
     6121 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6181 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6241 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6301 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6361 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6421 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6481 atctccgccg tgagaatgca ggtaaaggac cgtcggaaac ggtccttctt tttctggata
     6541 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6601 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6661 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6721 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     6781 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     6841 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     6901 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     6961 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7021 tcgaaaaatg gatccttctg aatgctgatg tccgttcatc tatcggataa gccttttttt
     7081 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7141 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7201 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7261 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7321 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7381 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7441 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7501 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7561 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7621 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7681 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7741 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     7801 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     7861 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     7921 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     7981 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8041 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8101 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8161 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8221 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8281 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8341 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8401 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8461 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8521 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8581 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8641 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8701 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     8761 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     8821 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     8881 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     8941 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9001 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9061 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9121 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9181 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9241 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9301 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9361 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9421 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9481 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9541 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9601 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9661 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9721 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     9781 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     9841 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     9901 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     9961 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10021 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10081 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10141 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10201 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10261 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10321 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10381 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10441 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10501 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10561 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10621 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10681 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10741 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    10801 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    10861 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    10921 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    10981 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11041 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11101 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11161 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11221 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11281 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11341 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11401 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11461 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11521 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11581 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11641 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11701 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    11761 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    11821 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    11881 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    11941 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12001 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12061 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12121 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12181 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12241 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12301 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12361 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12421 gtatataatc atttttatcg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12481 caaaacgata aagtttattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12541 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12601 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12661 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12721 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    12781 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    12841 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    12901 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    12961 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13021 gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc
    13081 atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa
    13141 gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta
    13201 tttttcaaaa ttatgctgcc attagcttta ccgtctattg ctactatctc gcttttttat
    13261 gctgttactt actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa
    13321 tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa
    13381 agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt
    13441 gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca
    13501 ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa
    13561 gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa
    13621 atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa
    13681 gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac
    13741 aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta
    13801 ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt
    13861 ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac
    13921 tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga
    13981 ttaaacggcg tttatcttgt tgattctgcg gcaataaaat cagccagcag caaaacatga
    14041 agtgctgttt tttgctgtcc tcttttattg cttgaatcag atcacaagaa aaacagcctc
    14101 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14161 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14221 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14281 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14341 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14401 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14461 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14521 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14581 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14641 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    14701 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    14761 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    14821 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    14881 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    14941 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15001 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15061 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15121 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15181 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15241 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15301 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15361 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15421 aatctgtccg aaaataagta ggaaaaaaga ccaatgagtc ctaccagtgt taaagacact
    15481 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15541 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15601 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15661 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    15721 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    15781 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    15841 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaact
    15901 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    15961 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16021 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16081 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16141 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16201 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16261 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16321 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16381 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16441 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16501 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatatcgcta
    16561 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16621 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16681 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    16741 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    16801 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    16861 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    16921 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    16981 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17041 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17101 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17161 tagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17221 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17281 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17341 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17401 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17461 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17521 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17581 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17641 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    17701 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    17761 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    17821 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    17881 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    17941 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18001 acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18061 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18121 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18181 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18241 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18301 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18361 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18421 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18481 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18541 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18601 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18661 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    18721 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    18781 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    18841 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    18901 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    18961 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19021 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19081 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19141 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19201 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19261 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19321 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19381 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19441 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19501 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19561 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19621 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19681 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    19741 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    19801 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    19861 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    19921 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    19981 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20041 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20101 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20161 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20221 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20281 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20341 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20401 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20461 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20521 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20581 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20641 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    20701 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    20761 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    20821 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    20881 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    20941 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21001 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21061 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21121 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21181 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21241 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21301 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcttacaga
    21361 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21421 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21481 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21541 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21601 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21661 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    21721 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    21781 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    21841 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    21901 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    21961 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22021 caa
//
LOCUS       DAFFQY010000044        21516 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFFQY010000044 DAFFQY010000000
VERSION     DAFFQY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.044"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="LRB02_001559"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="LRB02_001559"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL4659758.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="LRB02_001560"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="LRB02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL4659759.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="LRB02_001561"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="LRB02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL4659760.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="LRB02_001562"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="LRB02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL4659761.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="LRB02_001563"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="LRB02_001563"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL4659762.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="LRB02_001564"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="LRB02_001564"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL4659763.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="LRB02_001565"
     CDS             complement(8297..8767)
                     /locus_tag="LRB02_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL4659764.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="LRB02_001566"
     CDS             complement(8813..9238)
                     /locus_tag="LRB02_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL4659765.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="LRB02_001567"
     CDS             complement(9305..9574)
                     /locus_tag="LRB02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL4659766.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="LRB02_001568"
     CDS             complement(9585..11180)
                     /locus_tag="LRB02_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4659767.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="LRB02_001569"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="LRB02_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL4659768.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /locus_tag="LRB02_001570"
     CDS             complement(14733..15293)
                     /locus_tag="LRB02_001570"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL4659769.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="LRB02_001571"
     CDS             15646..16620
                     /locus_tag="LRB02_001571"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL4659770.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="LRB02_001572"
     CDS             16790..18190
                     /locus_tag="LRB02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL4659771.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="LRB02_001573"
     CDS             complement(18268..18990)
                     /locus_tag="LRB02_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4659772.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="LRB02_001574"
     CDS             complement(19069..19575)
                     /locus_tag="LRB02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4659773.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="LRB02_001575"
     CDS             19698..21497
                     /locus_tag="LRB02_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL4659774.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5973 a         4703 c         3864 g         6976 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttctcctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 ggatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa ataaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggtcga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctgggaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaata
//
LOCUS       DAFFQY010000045        21151 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFFQY010000045 DAFFQY010000000
VERSION     DAFFQY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.045"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..654
                     /locus_tag="LRB02_001576"
     CDS             <1..654
                     /locus_tag="LRB02_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659775.1"
                     /translation="KIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEV
                     IRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERI
                     DATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELE
                     KKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1357..1572)
                     /locus_tag="LRB02_001577"
     CDS             complement(1357..1572)
                     /locus_tag="LRB02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBL4659776.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1591..1827)
                     /locus_tag="LRB02_001578"
     CDS             complement(1591..1827)
                     /locus_tag="LRB02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBL4659777.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2085..4238
                     /locus_tag="LRB02_001579"
     CDS             2085..4238
                     /locus_tag="LRB02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBL4659778.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4207..5622
                     /locus_tag="LRB02_001580"
     CDS             4207..5622
                     /locus_tag="LRB02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBL4659779.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5655..6689
                     /gene="sppA"
                     /locus_tag="LRB02_001581"
     CDS             5655..6689
                     /gene="sppA"
                     /locus_tag="LRB02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL4659780.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6691..7212
                     /locus_tag="LRB02_001582"
     CDS             6691..7212
                     /locus_tag="LRB02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4659781.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7209..7595
                     /locus_tag="LRB02_001583"
     CDS             7209..7595
                     /locus_tag="LRB02_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL4659782.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8091..8163)
                     /locus_tag="LRB02_001584"
     tRNA            complement(8091..8163)
                     /locus_tag="LRB02_001584"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8128..8130),aa:Lys,seq:ctt)
     gene            8544..9113
                     /locus_tag="LRB02_001585"
     CDS             8544..9113
                     /locus_tag="LRB02_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL4659783.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9100..9960
                     /locus_tag="LRB02_001586"
     CDS             9100..9960
                     /locus_tag="LRB02_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4659784.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10150..10854
                     /locus_tag="LRB02_001587"
     CDS             10150..10854
                     /locus_tag="LRB02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659785.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10859..12694
                     /gene="walK"
                     /locus_tag="LRB02_001588"
     CDS             10859..12694
                     /gene="walK"
                     /locus_tag="LRB02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL4659786.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12691..14007
                     /locus_tag="LRB02_001589"
     CDS             12691..14007
                     /locus_tag="LRB02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659787.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14008..14877
                     /locus_tag="LRB02_001590"
     CDS             14008..14877
                     /locus_tag="LRB02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL4659788.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            14932..15741
                     /locus_tag="LRB02_001591"
     CDS             14932..15741
                     /locus_tag="LRB02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4659789.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15844..16473)
                     /locus_tag="LRB02_001592"
     CDS             complement(15844..16473)
                     /locus_tag="LRB02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL4659790.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16643..17935
                     /locus_tag="LRB02_001593"
     CDS             16643..17935
                     /locus_tag="LRB02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4659791.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18011..18673)
                     /locus_tag="LRB02_001594"
     CDS             complement(18011..18673)
                     /locus_tag="LRB02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4659792.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18898..19182
                     /gene="groES"
                     /locus_tag="LRB02_001595"
     CDS             18898..19182
                     /gene="groES"
                     /locus_tag="LRB02_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL4659793.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19233..20858
                     /gene="groL"
                     /locus_tag="LRB02_001596"
     CDS             19233..20858
                     /gene="groL"
                     /locus_tag="LRB02_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL4659794.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDDEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7206 a         3427 c         4264 g         6254 t
ORIGIN      
        1 aagatagtat tgaaaacaca aagtccgtta gaagttaaaa aatacctcaa acaattccga
       61 ccaaagcaag gaatatattt cttagatatt gatttaaatc atgaagttaa cggtatagaa
      121 ttagcagaag taatcagaaa atatgatgtt caagcaaaaa tcatttttac aactactcat
      181 gatgagatgt tacccgtaac aataaaaaga agagttgaaa cgttaggatt tgtaacaaaa
      241 gatcaaacac tagatgagta tcgaaacgag attgttgagt tattgttatt agcgcaagaa
      301 aggatagatg caacaaaaca ggttacgaat caagcatttg ttttttccat aggttctcaa
      361 acatttactt ttgatataaa tgacatttat tttgtagaag cttcaaaatt accacataga
      421 ttatcattgt gtactaaaga tgggcagtac gaattttatg gaagaattag tgagcttgaa
      481 aagaaatatc ctatgttaac aagaattagt cgagcgtgtt tagtaaatat ctttaacgtt
      541 aaagaaatag attttaaaaa acgttcacta tattttgatt cagagttggc cagaaacttt
      601 actttaggga aagcacaaaa aattaaggag aagttgaaag aaagcactgt ttagtggttt
      661 tttctttagg gagattgcat ttttctaata aaatagattc cttcactagt gcaaattttt
      721 ctgaatccta ttaaagttgt accaattttt atagctgtta ttagttacgt gcataacagc
      781 aatattagtt actggaggaa tatttctatg gtctggaacc aaaaaagggc caaatgaata
      841 gttcttttct tgtttttatt aagtaccata aaggggaatt aaaattaaat gtatggtgga
      901 agatgcgaaa gatttggcaa attctactaa tttttagaaa gtgtttttca ttttttagtt
      961 aaaaaatttg tcctgtcaag tgcaatccta tgattgtact tgataggatt tgagataatt
     1021 actttcctgt ttttataaat ttaacttgca ggaacaggct ttaatgcaca tccgacaagt
     1081 acattaccaa tacctcctgt aacggccgaa ataggtcctc ctgcagcacc aactacagcg
     1141 ctgcaagtcc ttttgtaaaa atatctctac cattacattt ccaaagaatt tttatctgac
     1201 tttaataaaa gataaaaatt taatataatt ataaaaacta gcacaagaag ctttagtaaa
     1261 aagttctgtc cattccaaac ttttgaaaag ataaagaaca tacctatgtt cgtaatcgac
     1321 acaaaccaat cttgaggttt gatatttttt aattgtttac gcaccggtcc acatgctgaa
     1381 tccagcacct aataaagctc cacctacacc acaagcagct attgcataag gtcctccggc
     1441 tgaacaaagt tttactcctg ctgttgcccc agaaaaagta gtgcctaaaa ctcccacggc
     1501 actcccacct tgaatatttt tcaactcttc tgaagataat ataaatgttg tttttgtgtt
     1561 tttaatatgc atactactta ttctcctttt ttattttttt cgagcatccc agccagctgc
     1621 agcacctcca gcgacagctc catataaccc tccaacaaca ccgcaagctg gtccgcaaat
     1681 tgctgttcct aaacctgcac cacctactgc ccctgtggta agtccaccta caacttgccc
     1741 caattttccc caagcattat tgcctccacc aacagtttgt gcatttccac aatacttagt
     1801 tcctttacat tttgcatgtt taaacacgct tctttaaata atatttttta cggtgctact
     1861 gtaacatacg tatttgatta acagtaaaaa tcttcctgaa acttatcaag tttattcttg
     1921 aatagtaaaa tttccaaaat aaatttgatt caggaatgaa aaagttagtg ttcaagaact
     1981 gttttcaaag tttttgtgga attatgagac aatattttca ctgtattaaa gctatattga
     2041 gaatttttct ataaaaattt tttagaagga gttgtgttag ataagtgata aaatcatttc
     2101 cttttgtgca gcaacaagat gaaaaagatt gtggtgttgc ttgcctttcg atgattttaa
     2161 aatattttaa aactgatatc ccgattcata aattacggga cctatctggg actgatttag
     2221 agggtacttc agcatttgga ttgaaaaaga catttgaaaa attgtctttt gattgtatgg
     2281 ctattcaagc agatagtgag atctggaaag aaagagatct tatcttacca ttaattgcac
     2341 atatattggt tgataacagc tatatgcact acgttgtaat ttataaaacg aagggaaacg
     2401 tactatggat tgcagatccc gctaatggaa aaaatagaaa aacgattgaa gcgtttcaaa
     2461 aagaatggac aggtattctt ttattaccta tgcctagaga aacttatatt cctattaaag
     2521 aaaaagtagc tggattaagt tcttttttgc ctataatttg gaaacaaaaa ggattagtat
     2581 tccacatcgt tttagcatcg attttcatta cgttttttgg cataggaagc tcatattatt
     2641 ttcaaggtat tcttgatttt ttcatcccta atcaagcacg ttcaacttta aatataattt
     2701 ccattggctt gatcgctgtc tatttatttc gggtattgtt tgaatatagt cgtagttatc
     2761 ttcttattat tttaggacag cgaatgagta tggcaattat gcttcagtat ttcaaacatg
     2821 tgttaacttt accaatgaat ttttttgcta cgagaaaatc aggggaaatt atttctcgtt
     2881 ttcttgatgc cagtaagatt attgatgctt tagcaagtgc tacgttatca atatttttag
     2941 atattggtat ggtgttgctt gttggggtta cgttagctat tcagaatagt acgttgtttt
     3001 taatcactct atgctctttg cctttttatg tagttgcaat attcgctttc gtgaaaagct
     3061 atgaaaaatc gaacagggaa gaaatggcag ccggtgcagc tgtaaattct agtatcatag
     3121 aaagtcttaa aggaatagaa acaattaaag catatagcgg tgaagagaaa gtatataacc
     3181 gtatagacca tgaatttgtt aaattaatga aaaaatcatt tcgtacggtt acgcttgata
     3241 atattcaaca aggaattaaa catttgattc aattaattag cagtgcatta attttatggc
     3301 taggttctta ttttgttatg gataatagaa ttagtctagg tcaacttatc acatacaatg
     3361 ctttactagt atttttgaca gatcctttac aaaacattat taatttacaa gtgaaaatgc
     3421 aaacggctca agttgcaaat aatcgtttaa atgaaatttt cgcgattgat tcagaaaaat
     3481 cgaaccagtc ttttgaatat atgctttctg aacaattatt tcaacaagat atcactgtaa
     3541 aaaacgtttc attctcgtat agcatgaagg ccccaacgtt gaacgaaatt tcatgtacaa
     3601 ttccttcgca cagtaaaacg gctatagttg gagttagtgg ttctggaaaa tcaactcttg
     3661 caaagttatt tgttcgtttt tatgaagcat cagaaggatc gatccattat ggaaacatta
     3721 atcacttaga cattcctttt cagatattac gcgatcgagt gacatatttg cctcaagagt
     3781 catttttctt tagtggaaca atcattgaga atcttatatt cggtttagat agcccaccaa
     3841 gtttcgaaca aataatagaa gtttgtgaga tagtccaact aaatgaattt attaatcaac
     3901 agccattacg ttacgagaca ttgttggaag aaggtggaaa taatctttca ggagggcaac
     3961 gacaacgatt agccttagca cgggctttat taaaaaatcc ggacatatta attttagatg
     4021 aagcaactag tggattagat accttgttag aacatgcaat tatgaattat ctcctcaatt
     4081 taaaagaaaa aacgattatt tttattgcgc atcatttacc aatagcaaaa gcatgtgatc
     4141 aaattttagt tctccatgaa gggcatttgg tagaaaaagg aacgcacgat gaacttcgtt
     4201 ttgccgatgg tatctatcaa aaattatggg aaatctagga gagggaaaca atttatgcag
     4261 aataataatt ggttcgaaca ttctagtatc tatactaaac aacatcatag cttctaccgt
     4321 tgggtgttat accctgtaat tcttttttta tgtattattg gattgttttt aacctttgct
     4381 aaaaaagagg ttgttattcg gacaacggct caattgaacc ccgaaaaaat agagaagtta
     4441 caagtgccat tagaagctaa aataatggaa aacttcctaa aagaaaatca atttgttcat
     4501 aaggacgatg tgttggttag attggattgt tctttaatag agaatgaaaa ggctcagata
     4561 gaacaagaaa accaacgtat tactcaacaa ataaagatgg ctcagctatt tattgaaagt
     4621 ataagtaaag gaaaaaattt gttttcaacg gatgacagtt ttggctacag taatcaatta
     4681 aagagcatgt tgtcagaaaa agaatcactc cgctacgctt tgaagcaaag tgaattaaat
     4741 gatcaaaagc aattagaagt atacgaaaag acaaaaagac aactagaaaa acaaattgag
     4801 agttcagata gtaaattaca agaatggcaa caagtacagg tagcttggag taataatcaa
     4861 tcattaaaag atttttcaaa agaaatgatg gcaaactatg agaattggca agaacaacta
     4921 aataatgttt ctgatgatca aaaaaatcaa gtgaaactga caatttcagc aagcataaat
     4981 gaacaaattg agcaactaaa aaaagaagta gaacagtatc agtcagaaaa agctaaatta
     5041 gttaaaccaa ctacttctga gaatgacaga attagtcaaa cggaaaaagg aaagcaagag
     5101 ctagaacaag caataacaac tgtaaaacaa acagttacag agctaaatga aaaacaagag
     5161 aagaaccagt cagttatcaa aagtttggac gatcaacttt caaaaggaat actaaaagct
     5221 cctgtatctg gaacggttca tttaaatgaa gaaacaaagg gacaagcaga actagccaaa
     5281 ggaacagtgc ttgcagaaat ttatccgatc cacgaaaaat caaaaatgaa atttacagca
     5341 cttttacctg cgaatgaaag tacctatatt aaagaaggga tgaaggttca ttttaaattg
     5401 gatcaaaaag gtggggctcc gattacgatc gacggaactt tagatgaaat ttcagaaaat
     5461 tctactgcca ctgagaaagg tgttttttat gcagtgaaag gaacgttaaa aacaactcaa
     5521 tcgtttccgt ttcgttatgg attaactgga gagttgtctt taattgtagg agaaaaaaca
     5581 tattggcaac aaattgtcga tgtgtttcta aataaaaaat aattattttt actaagaata
     5641 taggaggagt ttatatgaat aaaagaagat ggattgctgt gggagtagcg tgcggattat
     5701 tgttactatc cattatcgtc gcgctaattc caggaaaaga taaagaggaa gcatcaaata
     5761 caaccctaac aggaataaat aagatttttt acggatctaa cgaaatcaca gaagaaacgt
     5821 tagaagaagg cgcatcaaat aaaaagattg tcaaactatc cgtcaatgga gttattgcag
     5881 atacaggtga aagcaatctt tttagtaggg aacaatataa ccatcagaat tttttaacac
     5941 agctaaagaa aattcaagaa gacaaagcgg ttaagggagt gttattagaa gtaaattcac
     6001 ctgggggagg aatatatgaa agtgctgaga ttgctaaaga aatggcaaaa attaaaaagc
     6061 tagatattcc tatctatact gcatttaaaa atacagcggc aagtggaggg tactacattt
     6121 ctgctggttc agataaaata tttgctacgg aagaaacaac aactggatca attggtgtca
     6181 ttatttctgg tttaaattat tctggcttat tagaaaaact tggcgtaact gatgcgacgt
     6241 ataaaagtgg tgcattaaaa gatatgatgt cacctcaaca caaaccttca gaggaagaga
     6301 ataaagttat acaggaattt gtgatgagtg cttatgatcg ttttgtcaat gttgtagcaa
     6361 aaggaagaaa tatggatact aatgcagtga aagaacttgc ggatggaaga atttatgatg
     6421 gtaaccaagc agtagaaaat gggttggtgg atcaaattgg ttactctgaa gatgcattag
     6481 attccctaaa aaaagagaag aaattaactg atgcgacggt tatagaatat aaaaatgaca
     6541 caactggctt tgcttcttct tggttaggta ataagattgc tgaatggcaa gggctaaagg
     6601 caactacatc agatcgtgta atttcagttt ttgaaaaact tggattttct gatactccaa
     6661 aaccaatgta ttactatgga gggctataaa atgaataaga agagtggcat tgaaactttg
     6721 cctaattatg tatttgcggg attttggtta cgattttttg cttttcttat tgatttaatt
     6781 tgtattagca tcatcaccca tgctactata ggtctctttt acaaaatcat gggactggaa
     6841 tctgacaaat ccattattac tttttatggt atagcgtcat tagctattta tttaacctat
     6901 tttgtacttt tgactaagct gaattcagga caaacaattg ggaaaatgat ttttgggata
     6961 agagtagtga gctttgagga aaaagagcta agttggcaaa ctatctttat tcgtgagtgt
     7021 atatgtaggt ttatattaaa aaattttgta tttcaaattg ggtatttggt ggctgctttc
     7081 acgcccaaga aacaacatat tggggattat ttttctaaca cgtcagtggt tgttattcgt
     7141 tcattagaag ctattaacta tggtcaaaaa atttctaatt ctaaaacact taatctggag
     7201 agaattaaat gaataagaga aaaacagagt ttattttgat tttaattagt ggaataacag
     7261 ggattttatg tagtctttta gcattgatgg gtggtatgtt tctatcttct attgattcaa
     7321 ctactttaaa tactacagaa ctaaccagta aggatataga tatgttagtc aatctaagcg
     7381 ggaatgttgt tttatttgga ggaattaccc tagttttttc gataatgatt ttcataatag
     7441 gttttttagt aagaaaaaat caaaaaacag tggtctatgg tattagttta ctcattttat
     7501 ctattattcc attttttctt ttaacattat tgtgggtaat tcctggagtt ttaggtatta
     7561 ttgcgggatt gatgcttcta ataagaaaat cttaattagg caatgtgtag acaacgtgaa
     7621 acgattaatc aattaaaagc taaatacgtt ttgagaaatg ataacttgta gtcgatggaa
     7681 gacatgtaga taaaaaaact acgtgtcttt tatttattct aattttttcg aaaaaaagac
     7741 actctctgat tgttgtatta atggaaaagg ggaaagcagc aagtaaattt agtttttcta
     7801 ttaaatattc ttcacttttt aaccttttat agtacttaga tttaatccta tatgtttaga
     7861 gagttcattg gcagcatgat tttgcaaagc ttctcgaatt attgttatat tagaaacttt
     7921 actgttcata attatgcttc ttttttaact caaagtccaa agggctacag tggactttga
     7981 gttaaaaata aaaagcccta gttattttga aaaataaaaa aagaacagcg taaatgtttt
     8041 cgaaatgctt tcgcaccaac atttacctgt tccgtgggtt tggcacccaa tgggccgtga
     8101 ggggctcgaa cccgcgaccc gctgattaag agtcagctgc tctaccaact gagctaacgg
     8161 cccgtacaaa aagtacttaa taatagtagc actaagaatt ttaaaatgca agactacatt
     8221 taaagaaaat cagaaaaatc tttacttcct tataggaaat cattcataga ttattcacat
     8281 ttgctcgcta ttatataagc ataccaacaa acaaagttct tgcaagagaa caaaccctaa
     8341 cacttttttc attttttact cctttcccgc cgtctccccg gcgggtctcc tgttcttaac
     8401 gataaaagac aagaaggctt agccttcttg ttttttgttt tcatggaatc ccgctaatca
     8461 aatcacctag ttaaagcaac tagattgaat gtttatcata agtctgatat catgtttatg
     8521 aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca gcaattaatg gagtggggga
     8581 aggaactaga aacattccgt ctgcctcact gggaagaact ccctgactta gaactttata
     8641 tggaccaagt cagaacgtta gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc
     8701 atccattgct tacctcttct atggtaaaca attacgttaa actaggtttg ataccagcac
     8761 cagttaaaaa aagatataac aaagaacacg tagcattttt acttgcaatc actacgttga
     8821 aacaagtatt gactattcct gaaatcaaag aagggatatt gttccaagga aaaacagtag
     8881 gaattcgtga agcctataat ctattttgcg atgaacaaga agcagctgtg tggatggtca
     8941 gccaattagc acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc cctgtagaat
     9001 atattgctgt aagagcggca actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga
     9061 ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca
     9121 attttagtag attcaggtac ggatgttcca gaagaattga taaaaaaata cgagatgtat
     9181 atgatcccat tgaaaattat ttataaagat caggtatata cagataaaat tgacattacc
     9241 cctgaagaga tctatcaacg tttgccagag gaaatccctg gtacatcttt gccggatggt
     9301 gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg
     9361 gtaacaattt ccagtggatt gagcgggaca tataatgtgg ttcgtttaat tgcacaacag
     9421 caagaagaat tggaagcata tgtactagat acgaaaaata ttggtattgg tgcaggtttt
     9481 tctgcgatcc aagcagcaaa atggctggaa gaaggagtag aatgggatca actgatcagt
     9541 aatctgaatg agttagtcga aagaacaaaa gttttcttta atgttgcgac attggaatat
     9601 cttcaaaaag gtggacggat cggtttagtt gcttctattg taggaaccgc tttaaagctt
     9661 aacccaatca tctcatgtaa tgaagaaggg atttattaca cagtaggaaa agctagggga
     9721 cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag
     9781 gtcttcaact tagctgttgc ccatggggat gcgaaagaag aagcaattga aatggaagcg
     9841 agacttaaaa gagagttccc acaagcaaac caaatctact ttggccagat ttctccggca
     9901 ttagtagtcc acactggtcc gggattattg ggtgtcggtg tacaagtttt agaaggataa
     9961 atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc cttttttatt gaagttagaa
    10021 caaaaaataa taaagtattt tgcactttca ttttaatttt ctcttttgtt ttataggcaa
    10081 agatgaaaca aaagcagaaa tacggtataa tgagaaggta tgacaataat atataagagg
    10141 tggctcttta tgaaaaaagt tttagttgtc gatgatgaga agccgatttc ggatatcgtc
    10201 aaattcaatt tagccaaaga aggctatgac gtttatactg catatgatgg ggaagaagcc
    10261 ttggaaaaag ttgctgaggt agaacctgat ttgattttac ttgatttgat gctacctaaa
    10321 atggacggct tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc gatcattatg
    10381 gtaacagcca aagattctga aattgataaa gtcctaggct tagagttagg cgccgatgat
    10441 tacgtgacca agccattttc caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt
    10501 cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt gacgattggt
    10561 gatttgacga tccatccaga agcttacatg gtgacgaaac gaggagaaac aatcgagtta
    10621 acacatcgtg aattcgaact actcttttac ttggcgaagc accttggaca agtgatgaca
    10681 agagaacatt tattacaaac ggtttggggt tatgattatt ttggagatgt acgaacagtg
    10741 gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata atccaagcca tccaaattat
    10801 ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc cagaacagga gtaattggat
    10861 gaaaagaaaa gtccgctttt ttcgatcagt caattttaag atcgctatta cctttattct
    10921 gattttattg atctcgatcg aaattattgg tgcctacttt atacgtggtt tagaacgttc
    10981 cacgatcaat acatttatta aagatatgaa tcaaacggtg gaatcattag caacaacaat
    11041 cagcccggaa ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg cgaatatcaa
    11101 aagatttatt gaaaacagtg cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa
    11161 agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga
    11221 ttatgttgat atcaatgatt tcacgactaa acgttatgtt gctttagata acgacgataa
    11281 acgggtgaat atcaatgttc aaccgatcct gtccccgacc ggcgatgccg ttatcggggc
    11341 tctttacgtc aaaagtaata tcgaacaaaa atattcggaa atcaataaca cagcagtcat
    11401 tttctttact gcctcactaa ttgcggcatt tatttcaatg atcgtatctg ttttagttgc
    11461 tcgttcgatt acacagccga tcggtgaaat gagagaacaa gccattcgaa tcgctagagg
    11521 agactacagt cggaaagtca aagtgcatgg acaagatgaa cttggacagc tggctgatac
    11581 cttcaatcag ttagctgaac gtattgaaga agctcaagat acaatggaag ctgaacgcaa
    11641 tcgtttggat agcgtgctat cccatatgac agacggcgtc attgcgacag atcgcagggg
    11701 taaagtcatt acgatcaatg atatggctgt ttccttgcta gatatcaaaa acgaggaagc
    11761 aatcggacaa tcgattttga cacttttaga tatcgaagaa gaatatacgt tgcggaaatt
    11821 attagaagat ccaaacgaaa tggtacttga ccgttcaacc agtacattgg aaagtgatca
    11881 gatgatcttg cgagtagact tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt
    11941 agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt
    12001 cgtctctaat gtttcccatg aacttcgtac accgttgaca agtatgcgta gttatatcga
    12061 agcattgagc gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt tgaaggtaac
    12121 gctagatgaa acagaccgaa tgattcgaat gatcaatgac ctcttaaatc tatctcgcat
    12181 ggatacaggt aatacccagc ttcaattaga atatgtcaat ttcaacgagt tagtgaattt
    12241 tgttcttgac cggtttgata tgatggtggg aaatcaggaa aaaaattata agatccgtcg
    12301 tgaatttacg caaagagatc tttgggtcga agtagataca gataaaatca tccaagtagt
    12361 cgataatatc atgaataacg cgatcaagta ttcaccagat gggggaacga ttacttgtcg
    12421 cttattagaa acgcataata acgtgatttt aagtatcacc gatcaaggat tggggattcc
    12481 taaaaaagat ctgaaccgag tgttcgagcg gttctatcga gtagataaag cacgtgcaag
    12541 agcacaaggt gggacaggac ttggattagc tatttctaga gaagtgatta aggcgcatcg
    12601 aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg actttttata tctcgctgcc
    12661 atatgaacct tatgaggagg aatggtggga atgagaatct ctgaaaaagt cgttcgtata
    12721 ggactcattt tgatggttgc gttaagcata tacttctctt atgcaatctg gctgagccca
    12781 gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc aaatgatcga gtctcaaagc
    12841 aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta catggcacca ttcgggaatg
    12901 atccaagaaa ccaacagtga aaatctcgtg taccgattgc aattgatcat cgataaagca
    12961 agatttggaa agctttctga agtagtttca ggagacaaat cacagtttga acaggtgaaa
    13021 aatctgaact ctggttttga gttggcctat aatgcgcctt ttttgctaag agaatacaag
    13081 gaagcatttg atttagattt agatgtgtcg aacttggcaa aagaaagcga aaatatgtat
    13141 tttactagaa tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac
    13201 catctcgtat atgaagcaaa tatttcgttg aattggacag atatcaataa agaattaaca
    13261 gcaaaagaca caaaatggac agaaatgaca acagaaccat cgacgatcga cacgcaatat
    13321 gctacgaaac attcgatcaa actgaagaag tatagttata tcctgtcaca gcaaccctat
    13381 acacttttta gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc
    13441 aatgaattga ttttttacgg tgggaatgaa acaatgtcta tccattcgga aacgcaagta
    13501 gttgattttc gtggagagct gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt
    13561 ttcccctata ttagcaaatt aggcagttct ttagggaacc tcagatattt tgaccgcagt
    13621 gacaatacga tcgattatcg gatattcgtg gaaggcttcc cggtttttgg gacagacagc
    13681 agaggacaga ttggtgtgga agttggcggt gaaacgaatg gttctgtgca agtgaatatc
    13741 caaaccagtt tgaatacgat tcaagtgccg ataccttcag acgaagaagt agaacttgca
    13801 agtacgaatg aaatcatcca gcagttaaaa gctaagggag cagatagcaa caagattggt
    13861 tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc
    13921 ttacctgaat ggtatgtgaa atataaagat aactggtatt cagctaacga ccttctggaa
    13981 aaattacctg gattggaggc gaattaattg gatttcaaaa aaatcgaatg gatatttttc
    14041 gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt atcatgacgc ggtataccaa
    14101 gaaaacaatg tcattcgctc taatcagact gaatcaatcc aacaacgtct ggctagcgac
    14161 gatatcactt acacggggaa gatttcgtct gagaacaagc aaggctatta tcttagcgcg
    14221 gagcagacca atcttagcac ttcattagcg aattatcgca aggctggtaa aagcggcttg
    14281 ctggcaagtg gtgtgttttt agacggtgat gttttgacga aatctttatc cagttcagat
    14341 gctgaaaaga acgtgatcga tccagataaa attagtagtt ccttggaaga ttttttatct
    14401 aacaaagaca atattttatt cggtaatgat tatgtgtaca cgaaaaactt ttctcattta
    14461 gaagaagaac cactagaaat ccaagcaagc caagagtatg aaggcatccc attcaatgat
    14521 gatacagcaa aaatcgttct ttctttagaa aaaaacgata atgattatag catcacgaaa
    14581 tacatgcaga cgcatttatc tgatatcgag cagctgcgcg aaaagacgga actgtatacg
    14641 gaagaggaag caatcaatac attgtacatc aataacaaaa taccaagagg atcaaaaatt
    14701 ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt
    14761 cctgtttggc atgtagcgat cgaaacaagc gataaagtgg tccaaattga gagcatcaac
    14821 gctttcagca atacgatcgt gacaaacaat actgtaccaa aagtggaaga tcaataaaaa
    14881 agcgagtata atgaaaaaag tttacaaagt aagaagggaa gtaggagtca aatggctgaa
    14941 gaaaaaccag cttttaaaat cagtatccta gcaagtggaa gttcgggtaa ttcactttat
    15001 atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa aaaaatcact
    15061 tccttgatgg ctcaaattga tcggacaccg gaccaattag atggaatact tgtgacgcat
    15121 gaacatcgag atcatataca tggcgtgggt gtcttggctc gtaagtatca tttggatgtt
    15181 tttgccaatc agcagacttg ggaagcgatg gaaccactga ttggaaatgt accagtggaa
    15241 caaaaacatc tatttgagat ggggaaagtc agaacattcg gcgatatcga tattgagagt
    15301 ttcggcgtct cccatgacgc agcggctccg caattttatc gtttccataa agaaaatcgg
    15361 tcattcgtca tgttgacgga caccggatat tgcagtgatt atatgcgagg tgtcattgaa
    15421 aatgccgatg gttatttgat ggaaagtaat catgatcttg agatgttacg catgggacct
    15481 tatccgtgga atttaaaaca gcgtatttta ggagatagag gacatctttc caacgaagac
    15541 ggtgcacttg tcatgactga tgtgatcggt aatcggacca aacgaatcta tcttggccat
    15601 cttagcaagg aaaacaatat gaaagaactt gctcatttaa caatggaaaa tgttttgaaa
    15661 gaaaaagatt ttggcgttgg acatgatttt gaaatctatg atacagatcc agatgaagca
    15721 actgagctat tttcgattta aacagttcaa tcaataaaaa tataaaaagc agaagataaa
    15781 atcaatcagg gttggtttta tcttctattt ttattttttt agcatattaa aaaaataacc
    15841 gtattatcga ctgactacac tgatgtgctg cttttcatgt ttctcatcca gtatctcatc
    15901 tactaatgca atcgcgtaat ctgccatgct gatcgtactg tcgctagcag cgtttctttg
    15961 taaataatca tcacttaaca aataagtacc tgtacgtttg ccatgtggat caaataaaga
    16021 agctggactg atataagtcc aatgtagatt tttagattgt ttcaatttta acaatccttc
    16081 gtacatattg aaagctgttg gatagaaggg tgcattttta ggtacttggt caatcatacg
    16141 agtcgtcttt tctgtatcta agaataacga agaggcaccc ccaacgacaa ttaagcgtgt
    16201 atcggttcct tcaagcatct caatcaaatg ggacaaagaa gtttgatgca tttcttcttt
    16261 tcctttggga gcgttgaaag catcaacgac aacatcaaaa gatgcaagat cagtagttgt
    16321 caactcaaat aaatcctttt gtataaacgg aacatctgat ggcaattttt ctggatgacg
    16381 cacgattgcc gtcacgtcta aatctctgga caatgcttcg tctaaaataa atgaacctgc
    16441 atggccagta gctccaatga gtgcaatttt cataaaaaac ctcctttttt attcctgtcc
    16501 attgtaagca gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca
    16561 ttcctttcat ggttcgtgat cattgggtat agttaaatta agaaaactga ttgataaaaa
    16621 tagaagttga aggagataag aaatgaaaga gtggttagat cgtgcctttt taccaatgat
    16681 aacatctgtt gtgaatctta gacccttaac tgctttgcgt aatggaatga cttacacgat
    16741 cccaataatt attgttggct cgctgttttt cttatttgct cattttcctg tggattacat
    16801 cgctcagtgg atagacaaaa caggattttc tatgtatttt acacaagtat accaggctac
    16861 atttgatgtg atagcctttt ggatcgtttt tgccattgca tacgcttatg caaaagaaga
    16921 agacatcgat cgcttaccag tcggcataac agccttgtct gcttatctac tgcttattca
    16981 gccactaaat actcagatag atactgtctg gacaggtgct gaagggatac taggagcagt
    17041 tacaactggg ctcatcatcg gatggggcta tgcacgttta gtaagacttg cgaaagggtg
    17101 gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct tttacttcat tgattccaat
    17161 cattgttata gtcactttta cgcttatcgg ttcagcggtt tttcaacatt tgttcagaat
    17221 gactgttgtt caaggagtat ggacatatgt ccagcttccc attcaatcgt tcatagacac
    17281 gttgtttggt gtgcttttat taggtttttt tgttccgttt ttatggttat ttggtcttca
    17341 tggatctgcg atggtcaatg ggttggttag tcccgtcttg caagcgaatt cattagcaaa
    17401 tgcagaaatc ttagcctcag gacaagagtt gacggtggca aatggcggac atatcgttac
    17461 tcagcaattt ctcgatcagt tcatgacggt tacgggtgct ggattgacgt taggggctgt
    17521 attttttatg acggtttttg cgaaatcgaa gaaatacaga gaattaggaa atcttgctct
    17581 tttaccagcc tttttcaaca tcaatgaatc aattattttt tccacaccga tcgtgatgaa
    17641 cccgatgatg gctgttcctt ttattttcgc accggtcgtt tcaggattga ttacctattc
    17701 cgctttatat tttggattcg ttcctttatt tacagcagtc caagtccctt ggacaacacc
    17761 gccgatcctc tcgggtttct tagtaggcgg agtgcctgca gcgattttac aaggtatcgt
    17821 gttgtcaatt agttttttca tttatttccc atttttgaaa aaaatagatt ctgcaaactg
    17881 caaaaaggaa agacagacaa aaaatgatgg agcagcagaa aagatgaacg actaaaaaac
    17941 aaaaatactc ctgtcgcttt agattgggaa aatcaccaat ctaggatgac aggagtattt
    18001 atagtcatgt ttatactagc cctttttgga cagctaattg aacaagaaca actaatgtgt
    18061 tcattcctac gtgtgtgatc atcgatgtcc agatccgacc agttgctttg tactctaggc
    18121 tgaagaagaa acctaggaaa aagtagatta gcagatgacc atcattatga gcaaacgcaa
    18181 acagcaaaga gctgatcact gctccgaccc aaaaatttga atatcgactg atgatcccca
    18241 gtatactgcg gcggaatacg aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa
    18301 taaaagcggg tgcttccatg atcatctgga tgatattttg tgtattctcg gaaggggttg
    18361 ctttgccaaa caatacagat tctaacatga cagcgatgct ttgcagaatc aatgcgacaa
    18421 agattccaac caatccataa ataacaatct ttggagcaga agcttttttt accgctgatt
    18481 cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc taggacaacg gcgcctagta
    18541 tatacatgac tgcagttgct gtgatcaatt cagacgtaga ttttacaagt cctatcgaag
    18601 ctaggatcaa gggtgagata aaaattagac catagcaaaa aatgctgagg aagctatatt
    18661 tttttaaaga catgtaattc ctccttttaa gggataacaa gcatgcaagg caagcttata
    18721 tcgattgatt tcaattatat cataatttca ataagcagct gtcagaataa aatttctttt
    18781 ctttgattta aaagcttgca aaaaagaggg agaatgagta ttattaaaaa tgtaggttag
    18841 cactcgaagg ttaagagtgc taacagatta aaaaaagagt tatcggaggg atttatcgtg
    18901 ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc
    18961 ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc aaactgggac agttgttgca
    19021 gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc
    19081 gaccaagtta tgttcgaaaa atacgccggt acagaagtaa aatacgaagg aaaagaatat
    19141 ctgatcgtcg caggaaaaga tatcatggcg attgttgagt aaatttggaa gataaaaaaa
    19201 taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag
    19261 acgcacgcgc agctatgtta cgcggggtag ataaattagc agatacagtg aaagtaacat
    19321 taggacctaa aggccgaaat gtcgtactag aaaaatctta cggctctcca ttgatcacaa
    19381 atgatggtgt gacgatcgca aaagaaatcg aattagaaga tcattttgaa aacatgggtg
    19441 caaaacttgt atcagaagta gcatcaaaaa caaatgatat tgcaggagac ggtacaacga
    19501 cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg
    19561 ctaatcctct aggcattcgt cgcgggatcg aattggcaac aaaagcagca gtcgaagaat
    19621 tgcataatat ctcaactgtt gttgattcaa aagaagccat tgcacaagta gcagctgttt
    19681 cttcgggttc tgataaagtt ggacatttga ttgctgatgc aatggaaaaa gttggaaacg
    19741 acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac agaactagat gttgtagaag
    19801 gtatgcagtt tgatcgtggt tacttatcac aatatatggt aacagataac gacaaaatgg
    19861 aagctgttct agaaaaccca tatatcttga tcacagacaa aaaaatctct aacatccaag
    19921 atattctgcc attgttagaa caaatcttgc aacaatcacg tccattgttg attattgctg
    19981 acgatgtaga tgatgaagca ttgccaactc ttgtattgaa caaaatccgt ggaacattca
    20041 acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg cttgaagata
    20101 tcgctatttt gacaggtggt acagtaatta ctgacgatct aggtttagaa ttgaaagatg
    20161 caacaatcga aaacttagga aatgcttcta aagttgttgt agataaagac aatacaacga
    20221 tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc
    20281 aaattgctga aacaacttct gattttgacc gcgaaaaatt acaagaacgc ttagctaaat
    20341 tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat
    20401 taaaattacg cattgaagat gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg
    20461 tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa agtttctgct gtagaagcag
    20521 aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc
    20581 aaatcgctga aaacgctggt tatgaaggat cagtgattgt tgataaattg aagaacgtgg
    20641 aactaggcac tggtttcaat gctgctactg gagaatgggt aaacatggtt gaagcaggta
    20701 tcgtagaccc aactaaagta acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct
    20761 tgttattaac gacagaagca gtcgttgcag ataaaccaga accagcagcg ccagcagctc
    20821 ctgcaatgga tccatcaatg atgggcggta tgatgtaaga aaaagcaact tgcttatcta
    20881 caactgatca ttagtgaaag acagataaaa agaggctgga acagaagcgt ttaactccaa
    20941 gaaataagaa gaaattcacg gaaattgctt ttcaaatttt tgtgaatttc agcttatttc
    21001 cgaaggagtt gcttctgctt ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt
    21061 ctttactttt gtctcacgct cttttttgtg tgtaattata atgaaaatag ttatttttaa
    21121 tagttataat attttatcta ttaaaagtaa a
//
LOCUS       DAFFQY010000046        20801 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFFQY010000046 DAFFQY010000000
VERSION     DAFFQY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.046"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..300
                     /locus_tag="LRB02_001597"
     CDS             <1..300
                     /locus_tag="LRB02_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4659795.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="LRB02_001598"
     CDS             complement(724..894)
                     /locus_tag="LRB02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4659796.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="LRB02_001599"
     CDS             complement(1088..1267)
                     /locus_tag="LRB02_001599"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659797.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="LRB02_001600"
     CDS             complement(1277..1501)
                     /locus_tag="LRB02_001600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659798.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="LRB02_001601"
     CDS             1654..2766
                     /locus_tag="LRB02_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4659799.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="LRB02_001602"
     CDS             complement(2844..3137)
                     /locus_tag="LRB02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL4659800.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="LRB02_001603"
     CDS             3305..3619
                     /locus_tag="LRB02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL4659801.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="LRB02_001604"
     CDS             3640..5619
                     /locus_tag="LRB02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4659802.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="LRB02_001605"
     CDS             5632..5958
                     /locus_tag="LRB02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL4659803.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="LRB02_001606"
     CDS             5974..6492
                     /locus_tag="LRB02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659804.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="LRB02_001607"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="LRB02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL4659805.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="LRB02_001608"
     CDS             complement(7928..9433)
                     /locus_tag="LRB02_001608"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL4659806.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="LRB02_001609"
     CDS             complement(9525..10115)
                     /locus_tag="LRB02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4659807.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="LRB02_001610"
     CDS             10294..11940
                     /locus_tag="LRB02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4659808.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="LRB02_001611"
     CDS             11989..12708
                     /locus_tag="LRB02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL4659809.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="LRB02_001612"
     CDS             13187..14134
                     /locus_tag="LRB02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4659810.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGPFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="LRB02_001613"
     CDS             14146..15066
                     /locus_tag="LRB02_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4659811.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="LRB02_001614"
     CDS             15107..16564
                     /locus_tag="LRB02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4659812.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="LRB02_001615"
     CDS             16909..17523
                     /locus_tag="LRB02_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4659813.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="LRB02_001616"
     CDS             17516..19240
                     /locus_tag="LRB02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4659814.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="LRB02_001617"
     CDS             19237..20688
                     /locus_tag="LRB02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659815.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3512 c         4166 g         6414 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcccg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atattcatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DAFFQY010000047        20523 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFFQY010000047 DAFFQY010000000
VERSION     DAFFQY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.047"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..111
                     /locus_tag="LRB02_001618"
     CDS             <1..111
                     /locus_tag="LRB02_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL4659816.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            420..572
                     /locus_tag="LRB02_001619"
     CDS             420..572
                     /locus_tag="LRB02_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659817.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(569..844)
                     /locus_tag="LRB02_001620"
     CDS             complement(569..844)
                     /locus_tag="LRB02_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659818.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="LRB02_001621"
     CDS             1298..1504
                     /locus_tag="LRB02_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659819.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="LRB02_001622"
     CDS             1700..1867
                     /locus_tag="LRB02_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL4659820.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="LRB02_001623"
     CDS             1893..2237
                     /locus_tag="LRB02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL4659821.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="LRB02_001624"
     CDS             2242..2523
                     /locus_tag="LRB02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659822.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="LRB02_001625"
     CDS             2626..2940
                     /locus_tag="LRB02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL4659823.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="LRB02_001626"
     CDS             2918..4612
                     /locus_tag="LRB02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL4659824.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="LRB02_001627"
     CDS             4632..5810
                     /locus_tag="LRB02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4659825.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="LRB02_001628"
     CDS             5773..6459
                     /locus_tag="LRB02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL4659826.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="LRB02_001629"
     CDS             6459..7619
                     /locus_tag="LRB02_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL4659827.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLVVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="LRB02_001630"
     CDS             7629..8504
                     /locus_tag="LRB02_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659828.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="LRB02_001631"
     CDS             8501..8812
                     /locus_tag="LRB02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659829.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="LRB02_001632"
     CDS             8802..9155
                     /locus_tag="LRB02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL4659830.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="LRB02_001633"
     CDS             9145..9546
                     /locus_tag="LRB02_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659831.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="LRB02_001634"
     CDS             9539..9943
                     /locus_tag="LRB02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659832.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="LRB02_001635"
     CDS             9955..10563
                     /locus_tag="LRB02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659833.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="LRB02_001636"
     CDS             10583..10945
                     /locus_tag="LRB02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659834.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11147..14578
                     /locus_tag="LRB02_001637"
     CDS             11147..14578
                     /locus_tag="LRB02_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL4659835.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="LRB02_001638"
     CDS             14629..15366
                     /locus_tag="LRB02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659836.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="LRB02_001639"
     CDS             15376..17667
                     /locus_tag="LRB02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659837.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITACEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="LRB02_001640"
     CDS             17691..19817
                     /locus_tag="LRB02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659838.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19834..19983
                     /locus_tag="LRB02_001641"
     CDS             19834..19983
                     /locus_tag="LRB02_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659839.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            19980..20426
                     /locus_tag="LRB02_001642"
     CDS             19980..20426
                     /locus_tag="LRB02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659840.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..>20523
                     /locus_tag="LRB02_001643"
     CDS             20428..>20523
                     /locus_tag="LRB02_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4659841.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7228 a         3081 c         4291 g         5923 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctagttg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg cttgtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaa
//
LOCUS       DAFFQY010000048        20286 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFFQY010000048 DAFFQY010000000
VERSION     DAFFQY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.048"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            141..461
                     /locus_tag="LRB02_001644"
     CDS             141..461
                     /locus_tag="LRB02_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659842.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="LRB02_001645"
     CDS             913..1113
                     /locus_tag="LRB02_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4659843.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="LRB02_001646"
     CDS             1260..4049
                     /locus_tag="LRB02_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL4659844.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="LRB02_001647"
     CDS             4096..4623
                     /locus_tag="LRB02_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL4659845.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="LRB02_001648"
     CDS             4644..5834
                     /locus_tag="LRB02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4659846.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="LRB02_001649"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="LRB02_001649"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL4659847.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8225..8899
                     /locus_tag="LRB02_001650"
     CDS             8225..8899
                     /locus_tag="LRB02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL4659848.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8896..9237
                     /locus_tag="LRB02_001651"
     CDS             8896..9237
                     /locus_tag="LRB02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659849.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9267..9626
                     /locus_tag="LRB02_001652"
     CDS             9267..9626
                     /locus_tag="LRB02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL4659850.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10150..12231
                     /gene="cadA"
                     /locus_tag="LRB02_001653"
     CDS             10150..12231
                     /gene="cadA"
                     /locus_tag="LRB02_001653"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL4659851.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12542..14008
                     /locus_tag="LRB02_001654"
     CDS             12542..14008
                     /locus_tag="LRB02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659852.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14623..15162
                     /locus_tag="LRB02_001655"
     CDS             14623..15162
                     /locus_tag="LRB02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBL4659853.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15236..15676
                     /locus_tag="LRB02_001656"
     CDS             15236..15676
                     /locus_tag="LRB02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL4659854.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15689..15898
                     /gene="copZ"
                     /locus_tag="LRB02_001657"
     CDS             15689..15898
                     /gene="copZ"
                     /locus_tag="LRB02_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL4659855.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15898..18084
                     /locus_tag="LRB02_001658"
     CDS             15898..18084
                     /locus_tag="LRB02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL4659856.1"
                     /translation="MATAETKMDTFVITGMTCANCSTRIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18104..20275
                     /gene="copB"
                     /locus_tag="LRB02_001659"
     CDS             18104..20275
                     /gene="copB"
                     /locus_tag="LRB02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL4659857.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6778 a         3527 c         4406 g         5575 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag actatcatca
     5341 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     5401 atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca gaactgccgg
     5581 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg
     6721 atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtga
     6901 tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     7201 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     7261 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     7321 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     7381 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgcg cctgccgttc
     7441 agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat gactattgtg
     7501 acttgtgacc gaggagttgg ccggagagca ccgtttattc taaaaaagaa ctgcgaactc
     7561 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7621 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7681 ttacaaagaa ggtaattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7741 agcagcatct agcacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7801 attttcagca tgcaataatc catatgtaag ttcaattcac ccttgaaatc atcggaccag
     7861 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa agggacattt
     7921 actagaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     7981 tgacggaagc ttaggtgtga tcgatctctc tggaccaaac aacgaaggaa tcaaggggaa
     8041 tccggcacgt taagaaaaaa atagcataaa aaactagaca cttgatgagc gttcattcaa
     8101 ttgtttgaag ggactaggct ggtgtcattt tttatacata tattttctct ttcgtcttga
     8161 tagggcttat gggataatat aaggaagaaa atcatcgttt tattcaaaaa ggagtttttg
     8221 acaaatgaaa aaacgtatat tatttatcgg tgatagcatt acagacgcag gaagagaccg
     8281 agcagaagca actgatctag gtaaaggtta tccacttttg attgccggaa aactaatctc
     8341 ccaatatcca acgatggaat ttgaatgcat gaaccgtggg ataggaggag acaaaattgc
     8401 agatctgcaa gtacgctggc aagaagattg tttagcttta caacctgact atgtatcgat
     8461 tttgatcgga atcaatgata cctggcataa tactagcgat caggctatat tcggaactga
     8521 gaaatctgct cagcaatttg aatccgttta tcgaaaatta ctaaatgaac ttcaaacagt
     8581 gggaatctgc aacatcatct tgatggagcc gtttgtactt cctataaccg acgaccgtca
     8641 tggctggcgt aaagacttag accctaaaat ccaaattgtt cgtaaacttg ctaaggaata
     8701 tggcgcattg ctggttcctt tagatggtat attcaatgct ttagggattg tttatggttt
     8761 ttcgaaatat acaagagaag atggggtcca cccaacgatt ggcggacatg agatcattgc
     8821 acaaagctgg atagatgcaa ttgccgcagt cgattttttt agtggagata aaaatgagcg
     8881 aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa aagaagatct acctcaaatc
     8941 aatcagctga ttactgatca tccagaatca ttcgtaaaag caaaaacggc agctgaccta
     9001 aatgataaga ataaactatt gctcgttgct gtaacaaaag aaaaacggat tatcggattt
     9061 gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc agcgaccatc aggaaaacta
     9121 atccaacaca cattagtaac aaaagaaaat acggaagaaa tccaaaaaaa atttgcagaa
     9181 tatttgatac tggaagctaa aaaaagaaga ataaaatcat tttttgccca gttttaatca
     9241 aaaacagtaa tagaaggaga aatacaatga gttgtttagg aaatgtcatc tggtttatat
     9301 tcggcggact aatcggtggt cttagctggt tagcagcggg gatactttgg tgtatcacga
     9361 tcataggtat cccaatcggt atgcaatgtt ttaagatcgc ctccctttct tttgcgcctt
     9421 ttggcaaaga agtaatttat caccagtctg gcatgtcatt gattgttaat atcatctggc
     9481 tgatcgtttc aggcctacca attgctctcg cacatgtcgt cagcggcatc cttctatgta
     9541 tcacgatcat agggatacct tttgccaagc aatctttcaa acttgctcga ttggcgttga
     9601 tgccattcgg tgcagaaatt gtataaaaga gaataataaa ggttagataa agcatagagt
     9661 caggtgggat acatgtattt cacctgactc tatgcgttct cctgtataag ttcttccatg
     9721 tgacttttac gtcttttatt tatatggggt tactgtttat ggaaagttga aataattttc
     9781 attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat ctgggaagga
     9841 cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa tttagacaaa
     9901 ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa ggcaagtgcg
     9961 ggttggataa gagcagtggc aatgatcgaa aagaaaaaga aagaataagc caaaacatct
    10021 gtatggaaga aattttccga tgcagatgtt tttcattttt tctttcggat accttgacat
    10081 atgaataacc gttcatttat aattagttat gaagagatat tcatatgttg aggcgaggag
    10141 gtcattaaaa tgaaaagtta cagattagaa ggtttagatt gtgcgaactg tgcaatgaaa
    10201 atcgaaaaag gagttcagaa gatcaatgga gtcaaggaag caacggtaaa cttcacttct
    10261 ggaaaactaa cgattgacgc agaggaagat catttggcta cgattgaaca agaaataaaa
    10321 aaagtagtga aggaattaga accagatgtc aaagtaacag aaatagataa ggaaaaagta
    10381 tctgaacatg gaaatgaaaa agagagaaat acgctgttcc ggattttatt ttcccttgca
    10441 ggtatcgctt tattgctatt atttgatttt aatgaaccaa ttcgcttgat agggtattta
    10501 ttgatttatc tgctgatagg atatgatgta gtgaagaagg cagtgatgaa tatcgtcaaa
    10561 ggaaaaatct ttgatgagaa ttttcttatg tctgttgcta cgattggcgc tatgttgatc
    10621 ggtgaatatc cagaagccgt agcagtgatg ctattttatc aaatcggcga gtatttccaa
    10681 ggattggcag tcagtcattc aagaaaatcg attcgcgaat tgatggcgat ccgtccagaa
    10741 acagctcacg tgcagacgac ggaaggattg atgaccgtga accctgaaga cgtgcagatc
    10801 ggtcagtttg tacttgtgaa gcctggtgaa cgtgtaccgt tagatggaac gatcatcgaa
    10861 ggagaatcat tagtcgatac gtcagcactt acaggagaat cagtccctaa atctgtatat
    10921 gttggtgaaa cagtattaag tggatttatc aataaaaata aaccattttt agttcaagta
    10981 gaaaaatctt atgaaaattc tactatcagc aaattattag aattagtaga aaatgccagc
    11041 agtaaaaaag caccagctga aaatttcatt actaaattcg cgcgctatta cacaccagtc
    11101 gttgtaggat tggccgtttt attagctgtt ttacctccac ttgttgtttc tggagcggcg
    11161 ttttctgaat ggatctatcg agcattgaca tttttagtga tctcatgtcc atgtgcatta
    11221 gtgatctccg taccccttag tttctttggc ggtatcggag gagcaagtaa aatcggtgta
    11281 ttagtaaaag gttcaaatta cttggagttg cttgcacaaa ctgagacagt ggtattcgat
    11341 aaaaccggca cattaacgaa aggtgacttt tcgatccaga cgatcgatac aaaaatcgat
    11401 ccaaagatat tcatgcaata tgttgcgagt gcagaacagt tttctacaca tccgattgct
    11461 caatctgttt tagaaggcta cgcaggtccg cttttaccaa cagctaatat ccaagaattt
    11521 gcaggtgaag ggatcttagc tgaagttgat ggcaaacaag tattagcagg taatcacaaa
    11581 ttgatggaaa gatttgaaat ctcttttcct tcaagtcaag aaatcggtac attgctttat
    11641 ctagcgatcg atcaaagtta cagtggttat cttgttattg cagatacctt gaaagaagat
    11701 gccgtagacg cattggttca attaaaaaag gctggtgtga agaacacagt catgttgacg
    11761 ggcgattcga aaaaaattgc tgatcatatc gggaagcaag taggcgtgga caagatatat
    11821 agcgaattgc ttccagaaga taaagtgcag cggttagagg aaatcttaca aagaaacaat
    11881 aaaaagaccg catttgtcgg agatggaatc aacgatgcac cggtgctagc aagagcagat
    11941 gtaggaatcg caatgggtgg attgggaagc gatgctgcaa ttgaagcagc agatgtagtc
    12001 atcatgaatg atcaaccatc gaagattgca gaagcaatcc atttagcaaa gaaaacattg
    12061 aaaatcgtca aacagaacat tgtctttgct attgggataa aaattttggt acttgcctta
    12121 ggtgcttttg gttttgcatc aatgggagat gcagtatttg cagatgttgg ggtgacagta
    12181 ttagctgttt tgaatgccat gcgaagcttg catgtaaaaa ataaacgttg atcaatgaag
    12241 aggacgaatg aacgtcctct tttttttata tccttttctt aatgaaaaat gtaaagaaaa
    12301 tagataggat tataaaataa ctaatgtttt taattttgca gaaaattcaa aatagcttga
    12361 aattcaataa ggtaaatgaa ataaaagaaa ataacttatt tttttgatgg aaaaaatatg
    12421 aaaaaacgat gtgtattaaa taaaagaata cacacataaa ataacactgt cttgtaagtt
    12481 tgttaaaaat tgaatatgat tacatctgga taacaaattt ttatcataaa ggagagaacg
    12541 aatgaacaac aaacaattac gctggtttac tgttgcgtta attgcattta acatggtttg
    12601 gggcatgggg aatgtagtta ataactatgc tcagcaagga gtatcagttg tcacctcttg
    12661 gctattgatt ttggccattt attttattcc atacgcattg atcgttggac aattagggtc
    12721 agccttcaag gatagtaaag gaggcgtcag ttcatgggtc gaaaatacga catctaataa
    12781 acgactggca tactatgccg cttggactta ctgggtcgtc catattcctt atttggcaca
    12841 aaagcctcaa gcaatcctga ttgctggcgg ttgggcagtc caaggaaatg gaaatatcgt
    12901 aaatacgatg tcagtacaaa tggtagcagt tatttcactt attatatttc ttgccttttt
    12961 atatttatca acaaaaggac tttctacttt gaaagtgatt ggcggactgg ccggaacagc
    13021 gatgttcgtc atgtcgatct tatttattct tttagctgta acggcaccgt tcatcaatcc
    13081 aacgatggaa tttgccacac cacatatgaa tcaagtgaaa acatatattc caacatttga
    13141 tttcagttat ttcacaaccg tttcgatgct agtatttgca gttggaggag cggaaaaaat
    13201 atccccttat gtcaactcta caagaaatcc tgcaaaggaa tttcctaaag ggatgatctt
    13261 cttagcagca atggtaggga tttgtgctgt attaggctca gtcgcaatgg gtatgctgtt
    13321 tgctagcgat aatattccta aagacttgat ggcaaatggg gcatacagtg cttttcaaat
    13381 tttaggtaat tactatggta tcggtaactc attgatgatc atttacgcat tgacaaacat
    13441 gattggacaa atctccgcat tagccttttc aattgatgca ccactaaaaa tcttgttagc
    13501 agatgcggat ccagaatttg taccaaactg gctacgtaaa cgtaatcgta aaggtacatt
    13561 ggtgaatggt tatcttctga caggtatact agtaggaatc attattttgt tgccgatctt
    13621 tggtatcaaa gatatgaatg aattagtaaa atggttgacg aacttgaact cagttgtcat
    13681 gccaatgcgt tacctatggg tgttcttggc atatatgatg ttgaataaag cttataagaa
    13741 attcaattct gaatataaat ttgttaaaaa tccaaaaatc ggttttatct ttggtgcttg
    13801 gtgctttgcc ttcactgcgt ttgcatgtat cctaggaatg gtgccaaaag tagattatac
    13861 tgctgatcct aaagcatggt ggttccaatt attcacgaat atcatcacac caattgtctt
    13921 gatcttatta ggtatgatct tgccagcaat tgctcgtcga gacaaaaaca aagaaatcac
    13981 tgaaccagaa acagtggttc agccttaaaa tagaaatact tgttacaaga ggttgtgaca
    14041 gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt
    14101 gaagtttcgg cttgtttttc gcagaagcta cttctggaac accgtgaatc agaggttgtg
    14161 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagccc ctcatatttt
    14221 tcgttttgct gggcttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    14281 agtgagcctg ccgtttaact ccgagtcaca aggagcaatt tcaaaaaaca acttctcatg
    14341 tttgttgact attgtgcctt gtgaccgagg tgttggcagg agaacaccgt ttaaggaaga
    14401 ggttgtaaaa tcagcgattt gacctgagta ttagaggaac aaacgaaaaa atagctcctc
    14461 atattttgcc gttttttgag ttaatgctga aggtcagctg attgaacacc atttattcga
    14521 aacaagggac tttgacacga cttttgtcac agtcccttgt ttttttgcgg cgttataagt
    14581 ggtatgatcg aaccaaattc caaagagaag tgagggagaa gaatgaagat catcgtgatt
    14641 ggacaaagtg ggtcgggaaa atcgaccctc gcaaggaaga tcaaagaaat caccgacttt
    14701 ccgctactgc cgctagacct tttatggcat acaacagact acagtattca ggcaaaaaag
    14761 tggtttttgc aagaacagca atcgtttatg gtatctaatc cgtcatggat tgttgaagga
    14821 aattatactt ctacattaga ggaaaggatt aaagaagcag ataagatcat ttggcttaaa
    14881 gtgccgcgtt atctcgccat ctaccgagta gtttggcgtt ctctaaaaag aaagataaac
    14941 aaaaaatctc ggccagatat gcctgaatca ttttcagaga aactaaacag ggaatactta
    15001 gaatttttat cttttatctg gcattttgaa gaaaacaatg aaccgaaaat acagcagctg
    15061 atccaagtag ctaatgcaag aaataaactg atcatcctta aaacaagacg agagaaacaa
    15121 gaatttttaa ctaaattgaa aatagagagc aaaaataatt gacagttttt gtttacaagc
    15181 gtaatctgta ttcataagct aatgattaca tttgtaaacg aaaggtgtga aataaatgga
    15241 aaaagtcaaa acgccagtaa agatcagtga ttcagagtgg gagatcatgc gtgttatttg
    15301 gacacttgga cagacgacag cccaagaaat cacgcagatc ttagctgatt cgatggattg
    15361 gaaaccagca acgatcaaaa ctttgctggg acgtttggta aaaaaagaag tgatctggac
    15421 agaacaagaa ggaaaaaaat tcatttatca tccagctgtc tcggagatgg aaacagttcg
    15481 cagtgcgacg gaaaatcttt tttcccatat ctgtgcaaaa aggattggag agacgattgc
    15541 ggatctagta gaagaagcca ctttgactca agaagatatc aatgagattt tgaaacaact
    15601 ggaacaaaag caaccagtag atacgatcga atgtaactgt atcccaggtc aatgtgaatg
    15661 taaacaacat caataggagg aagaaaaaat gaaacaacaa ttttcaatcg aaggtatgag
    15721 ctgcaatcat tgtgcagcaa gagtagaaga aacagtaagc gcacttgacg gcgtgcaaaa
    15781 agtgaaagta aatttgaaaa aagcaaacgg tacggtcaag tttgatgaaa caaaagttca
    15841 atccgaaaag atttgtcaag caatcaatgg attgggttat aaagcagagg tgatttgatg
    15901 gcaacagcag aaaccaaaat ggatacgttc gtcattacag gtatgacttg tgcaaattgt
    15961 tctactagga tcgaaaaaga gctgaacgga caacctggtg tggtcaatgc cacagtcaac
    16021 ttggccactg aaaaagcaag tgtcaaatat gaagggacga ccactgaaaa attaatccaa
    16081 agcgtagaaa acatcggtta tggtgcaatt ttgtacgatg aagcccacaa acaaaaaatt
    16141 gcagaagaaa agcaagcgta tttgaaaaaa atgctattcg atcttatatt gagtacggtt
    16201 ttgactttgc cgttgatgtt gtcaatgatt gctatgatgt taggaagcca tgcggcgatt
    16261 gttcactttt ttcattttcc aatcgtccag ttagtgctgt ctgctcctgt ccaattttat
    16321 gtcggtgctc gtttttataa aggtgcgtac catgcaatca agacgaaagc accaaatatg
    16381 gatgtattgg tagcaattgg aacatcggct gcctttgcat tgagcattta caacggattt
    16441 ttccgtggac acccccaaga tctttatttt gaaagcagca gtatgatcat cacgctgatt
    16501 ttgctcggca aatatttgga acatacagca aaaacgaaaa caggtaatgc aatcaaacaa
    16561 ttgatgtctc ttcaaacaaa aaccgcccaa gtgataagaa acgggaaaga agaaacgtta
    16621 gcgattgaag aagtagtcgt tggtgatcaa ctagtcatcc gtcctggtga gcagattcca
    16681 gcagatggtc gaattatttc aggaagcagt gcgatagatg aaagtatgct tactggtgaa
    16741 aatcttcctg tagaaaagaa tccggatgac acgctttttg gcgggacaat caatacgaat
    16801 ggtctgctac acatggaagt cacacaagtc ggcaagcaga cagtattagc gcaaatcatt
    16861 caaatggtgg aagatgctca agggagcaaa gccccaatcc aaaaaatcgc agatcggatc
    16921 tcagggattt ttgttccgat cgtattagtc atcgcgttta tcacgctcat agctacaggt
    16981 ttgatcactg gtgattggca actcgcattg atccatagtg tctctgtatt agtcattgcg
    17041 tgtccatgtg cattaggttt agcgacccca acagctatta tggtaggaac gggtgttggt
    17101 gcacgaaatg gaattttaat taaaggcgga gaagcattag aagcagctgc tcacttagac
    17161 agcattgttt tggataaaac tggaacgatt acagaaggaa agcccaaagt tacagatcta
    17221 gtcggttcaa aagaagtcct atctattttt tatactttag aacaagcttc tgaacatcca
    17281 ctaggtaaag caatcgtgga atacggaaaa ctgcaagaag cagcaacata cgatatgatt
    17341 gattttacag cccacccagg tgctgggatc agcggtacga ttaatggagt acgctatttt
    17401 gcgggtacac gtaaacgatt aattgaattg aacctatcat ttgatgaata ccaagaacac
    17461 gcgttagaat tagaacagca gggaaaaaca gtcatgtttt tagctgatga aaagcaagtg
    17521 atcgggtgga ttgccgtggc tgatcaaatc aaactagaag ccaaacaagc aatcaagcag
    17581 ctccagaata aaggattaga tgtttttatg ctgacaggag acaacaaact ggcagccgaa
    17641 acgattggta aacaagttgg tattgatcca aagcatatct ttgctgaagt acttccagag
    17701 gataaagcag cctacgtaga aaaacttcag aaagatggca aaaaagtcgg tatggcaggc
    17761 gatggaatca atgatgcacc ggccttagca ttagctgatg tcggtatggc aatgggaagt
    17821 ggaacggaca ttgcgatgga aacagccgat gtgacgctga tgaatagcag tttggcatcc
    17881 attgcacaaa caatcgagtt gtctaggttg acattgagaa aaatcaaaca aaatcttttc
    17941 tgggcttttg tctataatac gattggtatt ccttttgccg cactaggctt tttgaatcca
    18001 atcattgcag gaggagcgat ggcgtttagt tccgtcagtg tattgttgaa ctccctgagc
    18061 ttaaatcgac acatgaaaaa gtaagagaag ggttggagta aaaatgaatg ggaacgatat
    18121 ggagaaaaaa catgagcata aaaaagaagt agtaaacaaa gaaagtacag aatctcatga
    18181 aaaacagatg gagatgaaac atgaccatag tcagatggat cattccatgc acatgggaca
    18241 taaccatgga gatatagcaa acagcaaaca aatggaccat tcgatgcaca tggatcacga
    18301 acacggcggc atggatcatt ccatgcacat ggggaatttc aaacaaaaat tttggctatc
    18361 gcttatccta gctatcccaa tcattctttt ttcaccaatg atgggaatgg aatttccatt
    18421 tcaagtaacg tttcctggtt ctgattggct cgtattgatc ttagcaacta tactatttat
    18481 ctatggcggt cagccatttt taagtggagc taagatggaa ttgaaacaaa aaagtcctgc
    18541 tatgatgaca ctgatcgcga tggggattac agttgcatat atttatagtg tctattcgtt
    18601 tatcgcaaat ctgctcaatc cacatacaca cgtcatggat ttcttctggg aattggctac
    18661 tttgatcgtt attatgctac taggtcactg gattgaaatg aatgctgtgt cgaatgccag
    18721 caatgcttta caaaaactag cagaattact gccagaatca gtgaaacgct tgacgaaaga
    18781 tggaaaagaa gagacagttt cattgaaaga agtaaacgaa ggtgatcgtt tgatcgtccg
    18841 gtctggtgat aaaatgccaa cagatggtgt aatcttgaaa ggtgaaacaa ttgttgacga
    18901 atcagctgtg actggcgaat caagaggaat caaaaaacaa gccaatgaca aagtgattgg
    18961 cggttctatc aatggggatg gaacgatcga aatcgaagta actggaactg gtgaaaatgg
    19021 ttatctagcg aaggttatgg aaatggtgcg aaaagcacaa ggagaaaaat ccaagctaga
    19081 gtcattatct gacaaagtgg caaaatggct gttttatgtc gcattaattg tgggtatcct
    19141 ggcattcatc gcctggttgt tcttgacaga tcttcctaat gcattagaac gtatggtaac
    19201 agtctttatc atcgcctgtc ctcatgctct aggtttagct atcccattag ttgttgctcg
    19261 ctccacatca atagcagcta aaaacggatt acttttgaaa aaccgcaatg cactggagca
    19321 agccaatgat ctagatgtga tcatgctgga caaaacagga acactaacag aaggaaaatt
    19381 tactgtaacg ggtgtcgaag tacttgatga tgcgttcaat aaaaatgaaa tccttcaata
    19441 tctaggagcc ttggaagcga atgccaatca tccattggct gttggcatta tgaattattt
    19501 gaaagaacat gaaatcaagc cttatcaagc tgaaaatcta aaaaatcttt caggagtcgg
    19561 gctagaggca acagtcaaaa aacaacaagt gaaaatagtt aatgaaaaag aagttgagcg
    19621 tctcggatta aacgtagaac aagcattatt gaaaccttac caagaacaag gaaataccat
    19681 cagcttcctg attttagaaa atcatctagc tgcaatcgtt gctttaggtg acgtagtaaa
    19741 aacagaagca aaagaattta tccgaacatt gaaagaaaga aaaatcactc ctgtcatgct
    19801 tactggtgac aataaaaacg ctgcccaagc agtagcagat tatttaggga tcgaagaata
    19861 ttatggcggt ttattaccag atgataaaga agctgttgtc cagaaatacc ttgaccaagg
    19921 gaaaaaagta atcatggtgg gtgatgggat caatgatgcg ccaagtttag cccgtgcttc
    19981 aatcgggatg gcaatcggtg cagggacaga tatcgcaatc gattctgcgg atgtcgtatt
    20041 gacaaacagc gatccaaaag atattttgca ttttcttgat ctagccaagc aaacgcgaaa
    20101 gaaaatgatc cagaacctct ggtggggggc aggttacaat attcttgcaa ttccgctagc
    20161 agccggaatt cttgcgccaa ttggtatcat attaagtcca gcagttggtg ctgtcttgat
    20221 gtcgttaagt acggtagttg tagctctaaa tgctttgaca ttgaaaatca aataataatg
    20281 aaaaaa
//
LOCUS       DAFFQY010000049        20003 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFFQY010000049 DAFFQY010000000
VERSION     DAFFQY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.049"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            324..1985
                     /locus_tag="LRB02_001660"
     CDS             324..1985
                     /locus_tag="LRB02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4659858.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2055..2402)
                     /locus_tag="LRB02_001661"
     CDS             complement(2055..2402)
                     /locus_tag="LRB02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL4659859.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2486..3427
                     /locus_tag="LRB02_001662"
     CDS             2486..3427
                     /locus_tag="LRB02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659860.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3428..4471
                     /locus_tag="LRB02_001663"
     CDS             3428..4471
                     /locus_tag="LRB02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4659861.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4477..5547
                     /locus_tag="LRB02_001664"
     CDS             4477..5547
                     /locus_tag="LRB02_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659862.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5537..6490
                     /locus_tag="LRB02_001665"
     CDS             5537..6490
                     /locus_tag="LRB02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4659863.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6502..7512
                     /locus_tag="LRB02_001666"
     CDS             6502..7512
                     /locus_tag="LRB02_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4659864.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7797..8618)
                     /locus_tag="LRB02_001667"
     CDS             complement(7797..8618)
                     /locus_tag="LRB02_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4659865.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8715..8993)
                     /locus_tag="LRB02_001668"
     CDS             complement(8715..8993)
                     /locus_tag="LRB02_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659866.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9261..11270
                     /gene="metG"
                     /locus_tag="LRB02_001669"
     CDS             9261..11270
                     /gene="metG"
                     /locus_tag="LRB02_001669"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL4659867.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11749..12336)
                     /locus_tag="LRB02_001670"
     CDS             complement(11749..12336)
                     /locus_tag="LRB02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659868.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12511..13833
                     /locus_tag="LRB02_001671"
     CDS             12511..13833
                     /locus_tag="LRB02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4659869.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13988..14764
                     /locus_tag="LRB02_001672"
     CDS             13988..14764
                     /locus_tag="LRB02_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL4659870.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14757..15326
                     /gene="rnmV"
                     /locus_tag="LRB02_001673"
     CDS             14757..15326
                     /gene="rnmV"
                     /locus_tag="LRB02_001673"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL4659871.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15319..16203
                     /gene="rsmA"
                     /locus_tag="LRB02_001674"
     CDS             15319..16203
                     /gene="rsmA"
                     /locus_tag="LRB02_001674"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL4659872.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16654..17283
                     /locus_tag="LRB02_001675"
     CDS             16654..17283
                     /locus_tag="LRB02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659873.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17406..18515)
                     /gene="ugpC"
                     /locus_tag="LRB02_001676"
     CDS             complement(17406..18515)
                     /gene="ugpC"
                     /locus_tag="LRB02_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL4659874.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18724..19146
                     /gene="mgsA"
                     /locus_tag="LRB02_001677"
     CDS             18724..19146
                     /gene="mgsA"
                     /locus_tag="LRB02_001677"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL4659875.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19469..>20003
                     /locus_tag="LRB02_001678"
     CDS             19469..>20003
                     /locus_tag="LRB02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL4659876.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6508 a         3594 c         4081 g         5820 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
       61 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      121 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      181 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      241 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      301 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      361 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      421 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      481 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      541 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      601 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      661 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      721 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      781 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      841 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      901 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      961 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1021 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1081 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1141 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1201 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1261 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1321 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1381 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1441 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1501 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1561 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1621 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1681 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1741 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1801 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1861 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1921 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1981 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2041 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2101 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2161 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2221 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2281 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2341 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2401 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2461 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2521 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2581 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2641 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2701 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2761 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2821 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2881 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2941 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3001 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3061 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3121 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3181 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3241 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3301 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3361 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3421 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3481 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3541 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3601 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3661 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3721 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3781 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3841 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3901 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3961 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4021 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4081 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4141 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4201 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4261 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4321 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4381 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4441 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4501 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4561 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4621 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4681 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4741 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4801 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4861 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4921 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4981 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5041 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5101 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5161 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5221 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5281 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5341 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5401 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5461 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5521 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5581 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5641 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5701 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5761 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5821 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5881 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5941 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6001 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6061 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6121 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6181 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6241 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6301 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6361 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6421 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6481 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6541 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6601 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6661 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6721 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6781 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6841 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6901 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     6961 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7021 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7081 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7141 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7201 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7261 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7321 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7381 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7441 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7501 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7561 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7621 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7681 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7741 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7801 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7861 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7921 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7981 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8041 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8101 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8161 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8221 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8281 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8341 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8401 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8461 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8521 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8581 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8641 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8701 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8761 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8821 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8881 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8941 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9001 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9061 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9121 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9181 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9241 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9301 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9361 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9421 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9481 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9541 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9601 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9661 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9721 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9781 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9841 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9901 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9961 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10021 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10081 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10141 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10201 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10261 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10321 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10381 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10441 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10501 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10561 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10621 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10681 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10741 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10801 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10861 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10921 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10981 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11041 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11101 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11161 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11221 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11281 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11341 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11401 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11461 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11521 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11581 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11641 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11701 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11761 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11821 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11881 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11941 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12001 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12061 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12121 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12181 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12241 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12301 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12361 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12421 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12481 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12541 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12601 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12661 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12721 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12781 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12841 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12901 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12961 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13021 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13081 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13141 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13201 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13261 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13321 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13381 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13441 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13501 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13561 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13621 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13681 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13741 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13801 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13861 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13921 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13981 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14041 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14101 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14161 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14221 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14281 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14341 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14401 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14461 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14521 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14581 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14641 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14701 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14761 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14821 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14881 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14941 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15001 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15061 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15121 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15181 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15241 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15301 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15361 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15421 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15481 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15541 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15601 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15661 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15721 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15781 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15841 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15901 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15961 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16021 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16081 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16141 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16201 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16261 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    16321 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16381 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16441 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16501 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16561 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16621 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16681 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16741 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16801 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16861 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16921 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16981 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17041 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17101 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17161 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17221 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17281 tagacatgat ctctcaaaaa aaaactctca tctagtaatg gataataaat cctctaagat
    17341 gagagttttt tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg
    17401 gcaatttatg gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg
    17461 atgttgaagg caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt
    17521 gagatgaact cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg
    17581 gaaacgacaa cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca
    17641 ctgtggatgt cttctggacg aataccgaag ataacttctt ttccttcata acctttttca
    17701 actaatactt tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata
    17761 acaccatttt tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca
    17821 acgaacatat tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat
    17881 ccgtctttca tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta
    17941 acatagatgg tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct
    18001 acacgtaatt tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc
    18061 cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt
    18121 tttcgtttca agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg
    18181 atgtctgctt tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc
    18241 atatgaggat acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc
    18301 acgtcattca tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc
    18361 gcgatcattc gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat
    18421 tcacggtctt tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat
    18481 tttttatata cgtttttcag agccatttct accataatac gttccacact ttctttttta
    18541 gttttattaa ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa
    18601 caagaatttt ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa
    18661 gaaataaaaa ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct
    18721 cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca
    18781 acagcgtata aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag
    18841 aggatcacgg aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg
    18901 gatcagcaaa ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga
    18961 gacccgctgg ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat
    19021 gtctatgaga ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg
    19081 gaagccggct tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca
    19141 ttttgatgga taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa
    19201 agctaaacaa actgataata tgcgatataa tacgaacgta ttggataata taaacaataa
    19261 tattagtttt tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga
    19321 tcccaaaaat aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc
    19381 ctcttggacg aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat
    19441 gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat
    19501 tgctattatg ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga
    19561 gaccccattt attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt
    19621 tggtcctttt tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt
    19681 ctcgaaatct gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta
    19741 cggattcttt ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag
    19801 tgtcacgctg atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt
    19861 gccattgttc agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc
    19921 gattcaatca attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag
    19981 aagtttagcg atccacgcta aat
//
LOCUS       DAFFQY010000050        19849 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFFQY010000050 DAFFQY010000000
VERSION     DAFFQY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.050"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..111
                     /locus_tag="LRB02_001679"
     CDS             <1..111
                     /locus_tag="LRB02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL4659877.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="LRB02_001680"
     CDS             124..510
                     /locus_tag="LRB02_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659878.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="LRB02_001681"
     CDS             1061..1285
                     /locus_tag="LRB02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659879.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="LRB02_001682"
     CDS             1303..1731
                     /locus_tag="LRB02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659880.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="LRB02_001683"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="LRB02_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL4659881.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="LRB02_001684"
     CDS             3139..4674
                     /locus_tag="LRB02_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4659882.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="LRB02_001685"
     CDS             4754..5632
                     /locus_tag="LRB02_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL4659883.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="LRB02_001686"
     CDS             5634..5951
                     /locus_tag="LRB02_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659884.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6078..6701
                     /locus_tag="LRB02_001687"
     CDS             6078..6701
                     /locus_tag="LRB02_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL4659885.1"
                     /translation="MIMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="LRB02_001688"
     CDS             6716..7606
                     /locus_tag="LRB02_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL4659886.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="LRB02_001689"
     CDS             7629..7844
                     /locus_tag="LRB02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659887.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="LRB02_001690"
     CDS             7856..8197
                     /locus_tag="LRB02_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659888.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="LRB02_001691"
     CDS             8197..8568
                     /locus_tag="LRB02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659889.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="LRB02_001692"
     CDS             8561..8956
                     /locus_tag="LRB02_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL4659890.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="LRB02_001693"
     CDS             8958..9335
                     /locus_tag="LRB02_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659891.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="LRB02_001694"
     CDS             9336..9944
                     /locus_tag="LRB02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659892.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10288
                     /locus_tag="LRB02_001695"
     CDS             9944..10288
                     /locus_tag="LRB02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659893.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKSK"
     gene            10254..10487
                     /locus_tag="LRB02_001696"
     CDS             10254..10487
                     /locus_tag="LRB02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659894.1"
                     /translation="MKLIQRRKKVSEALKSPSFKYSITYRELDIKTQLLEAGWTLPEI
                     ERTDLDELMRLFAFRDAVKEHEDVEYYDNFTQF"
     gene            10526..13315
                     /locus_tag="LRB02_001697"
     CDS             10526..13315
                     /locus_tag="LRB02_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL4659895.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13305..14045
                     /locus_tag="LRB02_001698"
     CDS             13305..14045
                     /locus_tag="LRB02_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659896.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14042..16804
                     /locus_tag="LRB02_001699"
     CDS             14042..16804
                     /locus_tag="LRB02_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4659897.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16817..17725
                     /locus_tag="LRB02_001700"
     CDS             16817..17725
                     /locus_tag="LRB02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL4659898.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17725..18342
                     /locus_tag="LRB02_001701"
     CDS             17725..18342
                     /locus_tag="LRB02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659899.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18346..18795
                     /locus_tag="LRB02_001702"
     CDS             18346..18795
                     /locus_tag="LRB02_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659900.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            18795..19319
                     /locus_tag="LRB02_001703"
     CDS             18795..19319
                     /locus_tag="LRB02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659901.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            19347..19754
                     /locus_tag="LRB02_001704"
     CDS             19347..19754
                     /locus_tag="LRB02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659902.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            19754..>19849
                     /locus_tag="LRB02_001705"
     CDS             19754..>19849
                     /locus_tag="LRB02_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4659903.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         7088 a         2787 c         4213 g         5761 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agctggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa gtaagtgagg cgctcaagtc accttcattt aagtattcga
    10321 ttacttaccg agaattagat ataaagacgc aattactcga agcaggttgg acgttaccag
    10381 aaattgaacg tactgacttg gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa
    10441 aagagcatga agatgttgag tactatgata atttcactca attttaggag gtgatacttt
    10501 gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat
    10561 gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa
    10621 tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg
    10681 agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt
    10741 tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa
    10801 ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact
    10861 tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga
    10921 tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg
    10981 ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa
    11041 agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc
    11101 tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttgt ctcaaggtaa
    11161 gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg
    11221 acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca
    11281 aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc
    11341 attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat
    11401 ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa
    11461 tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt
    11521 tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat
    11581 aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat
    11641 gggggcaata gctgctgcta attcatttgt tttaattatt ggtgcagtcg ttggtgcaat
    11701 agctgttttc ggttacttta tggcaaccaa tgaagagttt agaaacaagg ttatatctat
    11761 ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat
    11821 tgatacttgg aattctgcta aagaaatggc atcaaatgca gttgagggtg tcaaagatgc
    11881 ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac
    11941 aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc
    12001 atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag
    12061 agaaaaatgg aatgaaatta aaggttctat tatgtcggtt gctggtccat tgattacagg
    12121 tataaagaat gcatttttac atgttacttt ttatctagag accttatgga ataatttagt
    12181 tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt
    12241 tattacctcc atgatttctg gtggatggga agaaacaaaa aataatatga ttgctgtttg
    12301 gaataatatt aaagaaagtg ccataaatat ttgggaatct ataaagaata tatttgtaag
    12361 ttattttaca aatatttatt ttgctgcact caatatttgg acagggttca agcttacatt
    12421 gataaatatt tggaatgaag tggtaaatca agctaaatcg atttggttaa acataaagta
    12481 tttttttatt aatctttgga ttgatataaa atattttgca attcagaaat ggattgaatt
    12541 gaagttttct attattcaaa cttggatcga tttaaaatat aatgccatta ctacttggaa
    12601 taatattaaa cagtttttca aagatacttg gaaaaatatt aaagatacgg catacaatac
    12661 gtggatttct ataaaaaata ccatgattaa tacatggaat aacattaagg actctttctg
    12721 gaatattgtt actggaattg ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg
    12781 tgtttcaaat gctattaatc gggtaaaaga aatctttaat tcgttaaggg aagtcaattt
    12841 atttgaaatt ggcaagaaca tcattgatgg acttataaat ggtgtaaaag aaaagtggaa
    12901 tgctttgaaa gatactatca aaggtattgc aggtaatatc aaaaattgga ttaaaggagc
    12961 tttagatatt cattctccat caagatggat gcgtgatatg gttggtaaaa atattgttca
    13021 aggtattatc gttggtattg ataaagagca aagtaagtta gatcaaacca tgacaaattt
    13081 agtaaaaaca ccgtctgttc aacctgtagt aaatggaata agtcacctac caattacaca
    13141 aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt catttacatt taaatgttta
    13201 tggagattta cctgattcaa tggttcggaa gattaccaca aaaattaaaa cagaattgac
    13261 aagacaaatg aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa cttaagagag
    13321 gacaattttt tattaatcaa cattattctt ctgaatttaa tgtgtatatt caaaacaggc
    13381 ctgcttctgt ttcagctagt cgagtaattg aattgagaga gcgagaagga aatgattcta
    13441 tcattattga taaaggctat tacagaaatg tgactagaaa gattgagtgt tactataaag
    13501 caccttcaat tgatgtggtg caggagtggg aagatcgaat cactgaatgg ttagatatgt
    13561 cgtcttatag tgattttatt ctttactatg atgaacaata tatttatcaa gctgtagtaa
    13621 tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct tttgaattta
    13681 cagtgagcat tcgaccattt aaggaaaatt acaatggtag atttactatt cgacaaacag
    13741 aaacttttga aatttataac ccagagaagt attcttcaaa accgcttatt aaattgagtg
    13801 gttctggaga tgcttctttt tatattaata aggacaaata tgatttgaaa tcgttggaca
    13861 gagaattatt tatagattct aaattagaag aggcttaccg aaaattagat ggtaatttag
    13921 aacatcagga tcaagtcact ttatttttag attttccatt tttatatcct ggaaaaaatg
    13981 aaattaaatg gacgaaaaat attcattcat ttgaaataat gcctaggtgg tggagaaaag
    14041 tatgaaacca agaatatata gtcctactga aacagatttc tcaacgaatg gcttaggaat
    14101 tttaaaggat tgtacaagat gtgaaatata tgaagtagct aatggaaaat atgaattaga
    14161 gttggattat cctttaggaa ctagatttga tgaatatttt gaaaatgact atcaaataaa
    14221 agcaaagcca aacgatcaag aagagtatca tatctttttt attgatgata aagatataga
    14281 tactttttta aatacagtaa ctatttatgc tcagagtcgt acaaatcgac ttggaagacg
    14341 ggtagtcact cttgcggaag tagactctaa aactggtcaa gaagcgatat caattattga
    14401 aaccaaaatg gataaaaaat ctgacatacg actttattct gatattacgg ccgtttctag
    14461 cacaatcttt gaagcgagaa atgttttaaa ttgtattgct ggtgaacaag gatcattgct
    14521 tcaatattgg ggtggagaaa ttaaacgtga accatttaag ctttctttgt taaagcgaag
    14581 aggtcgcaat aatgttggaa cgattcggta tggaaaagat atgtctggtt taaaggtcaa
    14641 attagattgg acaggtataa aaacaagact tattccatat gctgatcctc aaagtgaggt
    14701 aggtacgact aatcgaattt atggttcgcc agtagatagt gcctatatta ataactatcc
    14761 tgatgtgtat acagagcatg ttcagtttac agaagaacaa ggagtaaaag acattaagag
    14821 cttaaataaa atagctaaaa attactttaa aacgatcaat cctggctgtg ataagcctaa
    14881 agtttctatt acggttgaat ttgataagtt gactgatagt gaagaagcga aagaatttgc
    14941 gaagattaga aattatggtc tgtttgatac atttaaaata tatcataaaa aatataatat
    15001 ttatcttgaa tcaaaggtta gtggactaca atacgattct ttatctgaaa aaactttgaa
    15061 attagaagca ggagacattc aagttgcttt ttatcagcaa caagctgtaa ctattcagga
    15121 taaattaaaa gattatgcaa cgaataatta tatgagtgat tttaatgatt atgtttcttc
    15181 aatgattaca ggtcaaggca gtgcaggagg atatgtaagt ttatggccaa aagaaaaacc
    15241 ttccaacatc tttattatgg ataatccaga tttagaaaag gcaagagaag tactaagaat
    15301 gaataaaaat gggattgctt tttctaagaa aggatggcgg gggcctttta cttcagcttg
    15361 gacattagat agtatattta atgccaattt tatacaaact ggattaatca aagcagatat
    15421 ctttcaaaat tcttttaaca agactggaga tgtattgaaa ttagttaatg gactactcca
    15481 aatttggaac aacaaaaaga aaataatgga gttaactaaa aaaggaatgg aattttggaa
    15541 ttctaagggt tcaattggaa cgattggaac aactgattct gctggtaatc cttttcctgg
    15601 ggcttctact ccaaccccta ttgaagataa ttctttagtt attcgtacaa atggagacgg
    15661 caaatatatt ttgatttctc ctaaagtagg aaaaggatta gttttattag gaaatggtaa
    15721 agcaatttat tttggagact tagatgtaca aggaaaactt acagttaaag gaaaagaaat
    15781 aattcctgga caaggtggtg gtccatcggg aggtggagaa tctactggtg ggtatcctag
    15841 tgaattaaaa acagatgcag aaaagagagc ttggagaatt tacgatattt tgtgtaataa
    15901 cggatttacg aaacaatctg catgtggaat attggggaac attcaacaag aaacaggagg
    15961 aacttttgat cctgatactg ttcaaatagg tggaccagca tacggattag ttcagtggga
    16021 tggttcttca tatcctcttg ttggtccagc aacatgggat ggaaaagttt atgttcaaaa
    16081 cttatttaat gctgcaggta ttaaagaacc aataacgagc ttagatgcac aagttcgatt
    16141 gcttatttgg acatttacaa atggacaatg gatgggcgta gtacaaccta cgacggttga
    16201 tggatttaag gcctgtactg atccaagaca agcagcatat gcttttgaac gaaactatga
    16261 acgtccggca gcgacacatc ctgaacgtca agattatgca gttaactggt ataacaaatt
    16321 taaagattta aaaccaggag gagctactgg agaagcagga ctaaaacatt tagagtcttt
    16381 gattggacaa cgaattggca atggtcagtg ttatggcttg tctgcagaat attcaggata
    16441 tctgggtggt tgtggcatgg gtgctggaac aaaatatggt ttaactcatg tgataggaaa
    16501 tacttctgca gcatctgata ttggtattgc ctatgattgg tctgctgttg gttggaaagt
    16561 gattcaaaac cctagatatg atcaattagt agttggtgca attattaatt gggcaagagg
    16621 tggacaagtg ggttcatggt ttgcagacgg aacttatgga catactggcg ttatccgagg
    16681 cttagctaat ggtcgtatgc aaacttatga acaaaacaca gagttaggta tgatttgtgg
    16741 aaagttagat cgccagtatt atagtgcaag tgcaatttct tccattgtca taccaccaaa
    16801 atagggaggt gatttaatgg caaagtggaa tgtcatatta agtacaacag aaccctataa
    16861 ttacgtgggg atgattcaag ttcgacaagg caataaaaac actgaagtta tggaagctac
    16921 aattgtcgaa aatggtcttc catatgattt atcagaatgt aaggtatatt ttgaatcagt
    16981 tgttggcgga aaatatccag tccaattaga aacaagaatt gtggatgcta aaaaagggaa
    17041 aatcaaatat atttttgata aatattccat gcagtgtttg catcgacaaa cagccaattt
    17101 cattatattt aaaggagaag acttgattgg aacaactcaa gacttctcct attttgtcat
    17161 caatgctgtt tcaaaaacag aaggagaaat gggttcttat tggcaatcaa ttgaagattt
    17221 aattgctgat atgacagatt ttattaatga aaataaaggc gattttacgg actggatgaa
    17281 tgaaaggaaa gaagaatttg atcgctggag agaagaacag gagaatagtt tccaagattg
    17341 gagagaagga caagaatcag attatttgtc atggtttgaa tcaatcaagg atattttgaa
    17401 gtctatcgat ccaggtggag taatgttagc cgaattaatg gatgcacgtg ttgatattca
    17461 aggagttcgt catgcgtcta tttcagagcg cttgctggca gatatggatt atctatatca
    17521 gaaattacga gcaacacttt tcacgattga atacggtgag attgaagtga ctgatatttt
    17581 gcaggatgat ctcttttcag ataatcacga agtcgaaaaa gttggaactg tagaatttcc
    17641 aattgaagaa ggagctttga ttattgcaac tgttgatgat ccaaaacaga atgttttcac
    17701 tcttgagaaa gttggggtga tttgatggtt aaagtaaaga gaatgatgga gactgatgaa
    17761 tctggtgtta aacgtcaatt tatgccaatt acacatgttt ctgcagtttt aggcttagaa
    17821 aaaatgatat caggacaatc aaaagttctt tcggttaatg gtaaatatgg tgcagtcatt
    17881 ttaacgaaag ctgatttagg attagagaat gcaattactg aactacctta tgcaagcgag
    17941 acaagcgatg gtattcttac tgctgaaatg tttcaaaaga tcgtaaatgg agaaggtgga
    18001 acatatattt tgccgattgc tactcctgaa caattaggag gtataaaagt tggggaactc
    18061 ttagaagtta ctgaagaagg tgttttatct gcaactaagc aaacagactt caatttctct
    18121 gaagaattaa agggaaaatt agaatctcta aaaatattaa aagcaggtgc aaatatatca
    18181 attgcagaag atggaacaat tagttctaca ggtggtagtg ggactggtgg tgttaatcag
    18241 tcatatgtag atcaaaaatt ccaagaagct gtaaatcaag cagaaagtta cacaaacgaa
    18301 agaattccaa attttacttt tgaaaaaatc ggggaggtat agaaaatgac agatatcgta
    18361 aaaataaaac aaagcggtgt acaggtttat ccacaaactc attggaatgc tatagaaggc
    18421 aaaccaacaa cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata
    18481 ggaaccgttt ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct
    18541 agatttaatt ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact
    18601 acagctgtag ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg
    18661 gatggaattg cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt
    18721 ttaatgtcag caaccgataa agtgaaactc gatggattaa ccaatattac atttgagaaa
    18781 gtggggacgg tttaatggct gatattgtgc aattaaaaga agacggagtt gctaagtatt
    18841 tgaaaactca tgccaaagga attgatggtg tggagggtgt tttagtaaaa gccacaggta
    18901 atgaaacggt cttaggaacg aaaaatttta aagacggatt gcaattcaat ggattgcctg
    18961 tgcaagcagg tatgatcgag cgagctatca cactagctga tcgaagtgat acaacaaacg
    19021 taactgatgt gaatggaaaa ataatcagaa tcggaaacat cgtatttctt acttttaatt
    19081 ttaaatgtgg cacatggccc gaaggatcag aaactcgttg gattttaaaa atcccggacg
    19141 gtttcaaacg tgatcaaggc tatccagcgc aaaccgcgct ttcactagtt agaaatgcaa
    19201 gtcagccagc ggatgcacgt gcattcattg atcaaagtag catcatacaa gccaaatcag
    19261 gttcaggtag ttcatatatt tcaggtatgt ggatcactca agatccttgg ccggcttaac
    19321 agattactaa ttggaggaag agtaaaatga aaacaattta taaagtattg tatccaatgg
    19381 gatatgaaga acatcaagta gaagataact ttccaacatc tttaccattt gtcgaagttc
    19441 cacctatttt gtttgaaaag aaggaagatg aaacggatga aggtttcgga agaagacaac
    19501 agtcgcaatt ctttaacttc actgaaaata agtgggaaga agcagttaca caagattatt
    19561 caaaaaaatt agaactacta gaaaatttat ctataggttt acaggtagat aatgctgcat
    19621 tgaaaaaatc aaatgaagag ttaacggaaa aagcagattc gatggcgcag ttgaacgcca
    19681 aactgatgct taatgattta acaattaata aaaaaattga agcaatagaa aaacaaattg
    19741 gaggtgcttc ataatgttta gttttagcga tgtgaaaatg atgtatgatt ggggctgttt
    19801 tactgacgat caagttcgac aattcgttcc actatgcatt acagacgaa
//
LOCUS       DAFFQY010000051        19446 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFFQY010000051 DAFFQY010000000
VERSION     DAFFQY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.051"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            127..3654
                     /locus_tag="LRB02_001706"
     CDS             127..3654
                     /locus_tag="LRB02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL4659904.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="LRB02_001707"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="LRB02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL4659905.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="LRB02_001708"
     CDS             7442..7783
                     /locus_tag="LRB02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4659906.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="LRB02_001709"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="LRB02_001709"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL4659907.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="LRB02_001710"
     CDS             9140..11560
                     /locus_tag="LRB02_001710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL4659908.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LRB02_001711"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="LRB02_001711"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL4659909.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="LRB02_001712"
     CDS             12994..13731
                     /locus_tag="LRB02_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4659910.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="LRB02_001713"
     CDS             13745..14560
                     /locus_tag="LRB02_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL4659911.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="LRB02_001714"
     CDS             14573..15220
                     /locus_tag="LRB02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4659912.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="LRB02_001715"
     CDS             15236..15892
                     /locus_tag="LRB02_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4659913.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="LRB02_001716"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="LRB02_001716"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL4659914.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="LRB02_001717"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="LRB02_001717"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL4659915.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="LRB02_001718"
     CDS             18234..18752
                     /locus_tag="LRB02_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL4659916.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="LRB02_001719"
     CDS             18859..19359
                     /locus_tag="LRB02_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4659917.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4175 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggaca
//
LOCUS       DAFFQY010000052        18773 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFFQY010000052 DAFFQY010000000
VERSION     DAFFQY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.052"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(565..1131)
                     /locus_tag="LRB02_001720"
     CDS             complement(565..1131)
                     /locus_tag="LRB02_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4659918.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="LRB02_001721"
                     /pseudo
     CDS             complement(1131..2087)
                     /locus_tag="LRB02_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            complement(2087..2743)
                     /locus_tag="LRB02_001722"
     CDS             complement(2087..2743)
                     /locus_tag="LRB02_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL4659919.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3113..5131
                     /locus_tag="LRB02_001723"
     CDS             3113..5131
                     /locus_tag="LRB02_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4659920.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5201..5407
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5558..7972
                     /locus_tag="LRB02_001724"
     CDS             5558..7972
                     /locus_tag="LRB02_001724"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL4659921.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8320..9873
                     /locus_tag="LRB02_001725"
     CDS             8320..9873
                     /locus_tag="LRB02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL4659922.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10254..12038)
                     /locus_tag="LRB02_001726"
     CDS             complement(10254..12038)
                     /locus_tag="LRB02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659923.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12053..13774)
                     /locus_tag="LRB02_001727"
     CDS             complement(12053..13774)
                     /locus_tag="LRB02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4659924.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13874..14635
                     /locus_tag="LRB02_001728"
     CDS             13874..14635
                     /locus_tag="LRB02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4659925.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15602..15870
                     /locus_tag="LRB02_001729"
                     /pseudo
     CDS             15602..15870
                     /locus_tag="LRB02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16542..>18773
                     /locus_tag="LRB02_001730"
     CDS             16542..>18773
                     /locus_tag="LRB02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659926.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6173 a         3329 c         3772 g         5499 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggctatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataattatta ataatagata aattgaagtt ggaggataga agatggaaaa
     3121 cattgcaaga aaagaacgga gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg
     3181 aggggcagca atcgtgggaa ctgcgatggt aggctgttct gtcgctgccc cattagtaca
     3241 gccggttcct gttcaagcgg atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc
     3301 ggcatttatc acagaaattg ctacttatgc tcaaccaatc gctcaagcca acgatctata
     3361 tgcatcagtg atgatcgcac aagcagttgt agaaagcggt tggggcagta gtgcgttatc
     3421 tcaagcacct tattataatc tttttgggat aaaaggcagc tatcaaggac aaaccgtcta
     3481 catggatacg cttgaatttt taaataatca atgggtaaat aaaaaggagc ctttccgaca
     3541 atatccttct tttgctgaat ctttcaatga caatgcctat gtacttagaa atacttcatt
     3601 tggcaacggt tattactatg ccggtacttg gaagagcaac acgaaatcct atacagatgc
     3661 gacagcttgt ttgacagggc gttatgcgac tgatcctggt tacgcaggca aattaaataa
     3721 tatcatcgca acttacggat tgacccaata tgatacacca gctagcggaa acgcaggcgg
     3781 tggagcgact acaggtggca ataatactgg aagtgcaggt agtactggcg gttcaagcaa
     3841 ttctggtaat acaaactcag gttcagcaac ttcgaccact tatactgtcc aatctggtga
     3901 ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg gatcaattga tccaatggaa
     3961 caacatccaa aacaatttca tttatccagg acaaaaactg acaatcaaag gtagccagga
     4021 aaatggctct tcgacgaata attccggtaa caataccaac tcttcaggaa atgctggtac
     4081 atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt caatctggtg attctgtttg
     4141 gaagatctca aatgatcacg gaattacgat ggctcaattg attgaatgga acaatatcaa
     4201 gaacaatttc gtttatccag gacaacagct aatcgtatca aatggcggtt cttctaatgc
     4261 tggagcaaat actggctcaa atactgggaa tacaagtggc tctagttcgt ccagctcaag
     4321 ttcttctaat acagcttcag gaacgaaata tacagttaaa gccggagact ctgtttggag
     4381 cgtctcaaat aaatacggaa ttacgatggc tcagctaatc caatggaaca atatccaaaa
     4441 taactttatc tacccaggac aacaactgac tgtttcaaac agtggatcaa caaacagcac
     4501 gtcaggtaat acaggttcgt caaccaatac aaataacagc agttcagtta atactggcag
     4561 cagttcgtct caaggcactt ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa
     4621 taaaaacggc atcacgatgg atcagttaat cgaatggaat aatatcaaaa acaacttcat
     4681 ttatccagga cagcaattga tcgttaaagg tggaacttcg gcaaatacaa atacaggttc
     4741 aactacttct gcagcgaaac caaatacacc gaatacgtct gctacgacaa gtacatcatc
     4801 cactggtaat acgatgtata ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa
     4861 acatcatatc acgatggatc agttaatcga atggaataat atcaaaaata atttcatcta
     4921 tccaggacag caagtgattg tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt
     4981 tactggttcg aaaacctata ctgtgaaatc tggagaatct gtttggagtg ttgctgacag
     5041 tcatggaatt acaatggctc aactaatcga atggaacaat attaagaaca actttattta
     5101 tccaggtcaa acgttgattg tagctaaata aaatagctgt tcgaagaaat aacgattgaa
     5161 ttccacaaaa atatttgtta ctataaggct acttaaatat gacaacactt gaaaaggaag
     5221 tagtaaaaag actgaagttt cagagagcgc atggctggtg agaatgtgca cggatgcttt
     5281 tgaactcgcc tttgagttgc ccttgctgaa tacccgagta agcaagcggc ggtgacggcc
     5341 gttacaacgt tcgaggcctt ttcactaagg taaaattagg tggtaccgcg tagattacgt
     5401 cctataaaaa gagcaatctt tttataggat ttttttattt cttttgtgaa gtttgagaaa
     5461 acaaagcaaa aggaaataaa aataaacaga ttctgccggt tttttcgtta tatttataat
     5521 agaaacaagt gttttcaata gaacaggagg acagcaaatg agttacaatc ataaagaaat
     5581 tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact ttcaacaccc atgatgatcc
     5641 tgaaaagcca aaattttacg cactggatat gtttccctat ccatccggtc aagggctaca
     5701 tgtagggcat cctgaaggtt atacggcaac ggatatcctt tcacgtgtga aaagaagtca
     5761 aggatacaac gtgctgcatc caatggggtg ggatgcattc ggtctgcctg ctgaacaata
     5821 tgcgctagat acaggaaacg atccagctga gtttacccaa aaaaatatcg aaacattccg
     5881 acgccaaatc aattctttag gatttagtta tgactggaat cgcgaagtga atacaactga
     5941 tccggagtat tataaatgga cacaatggat ctttacgaag ctttatgaaa aaggtctagc
     6001 ttatgaagca gaagtagcgg taaactgggt acctgaatta ggaacagtta tttcaaatga
     6061 agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg
     6121 ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta ttggatgatc tagaattagt
     6181 tgattggcca gaaagcatta aagaaatgca aagaaattgg atcggtcgtt cagtaggagc
     6241 aaatattgaa tttaaagtgg ctggtacgga taaatcctat accgtattta cgacacgacc
     6301 agatactcta ttcggtgcaa cttactcggt tcttgcacct gaactagatc ttgttagaga
     6361 aattacgacc cctgaacaaa aagctgctgt agaagcttat atcgaagaga cagctaaaaa
     6421 atcagatcta aagagaacgg atcttgcaaa agaaaaaaca ggagtattta ctggtgctta
     6481 tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg atcgccgatt atgtattatc
     6541 ttcatacggc acaggagcca tcatggctgt tccagcacat gatgaacgtg actttgaatt
     6601 cgctcaaact ttcggactag aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc
     6661 tgcttacaca gaagatggta cgcacatcaa ctctgaattt ctagacggca tgaacaaaca
     6721 agaagcaatc gataaaatga atgaatggct agaagaaaat ggcgtaggga aaaaagaagt
     6781 cagctatcgc ttacgtgact ggctattctc ccgtcaacgt tactggggtg aaccaatccc
     6841 aatcatccat tgggaggacg gaacagtcac agctgtccca gaagaagagc taccattacg
     6901 gttgcctaag acaaaaaaca tcaagccaag cggtactgga gaatctccat tagcaaatat
     6961 cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa
     7021 tacaatgcca caatgggcag gaagctcatg gtattacctg cgttatatcg accctcataa
     7081 caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg ctaccagttg acatctacat
     7141 tggtggagca gaacatgcgg tacttcattt actatatgca cgattctggc ataaattctt
     7201 atacgatatc ggtgttgttc ctacaaaaga accattccaa aaactataca atcaaggaat
     7261 gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga
     7321 tgatgtggtc gaaacttacg gtgcagatac actgcgcatg tacgaaatgt tcatggggcc
     7381 attagatgct tcgattgctt ggagcgaaaa tggcttagaa ggaagccgta aattcttgga
     7441 tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg cgtgaccgta tcactacatt
     7501 gaatgatggc aagcttgata aagtgtatca tcaaacagtg aaaaaagtga cagaagacta
     7561 tgaaaatctt catttcaata cagcaatctc ccaattgatg gtctttatca atgaagccta
     7621 taaagtggac gctctgcctt atgaatatat cgaaggattt gttcaactac tcgcaccgat
     7681 tgctcctcac atgggtgaag aactatgggc aatccttgga aatgatggtg gtatttctta
     7741 cgcaccatgg ccaacatacg atgaagcagc acttgttgaa gacgaagtag aagttgtttt
     7801 ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc cgagacttag gaaaagatga
     7861 gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt
     7921 cagaaaagtt attgcggtac caaacaagtt agtcaatatc gttgctaatt aattggctct
     7981 ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg ggccagaatg aacttcattc
     8041 tgtctcagat tttttgttat ttcaaattga ttaattgatt gatcaaaaag gttttgattt
     8101 tcatgttctc aaacacgttc aatgtctttt tcttctgaat ggtcttgtaa gagatctctg
     8161 taatcatcaa atttttgtct attaatctta gtattttttg atgtataaac tacctgaaat
     8221 tgagagtaca taccaaattc ataaattttt tattagtggg actagtgatt gtatggattt
     8281 tgacgcataa tgaatgaagt atgagaaaag gagagacaaa tggatcaaga actatttatt
     8341 atcgaagtat tatcagctaa acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga
     8401 ataatgttca agcttttaag cttagtattt ttctctggtt tgcttttttt cggagctgta
     8461 tcgttcctat atcgccatat gagtgcagta gtccgttcag ctgaagggat gcaaaaatta
     8521 aataatttgt atcacttttc gtggggagtt ggcggaataa caattgttgt gcttattgtt
     8581 tttcttctac ttatttggct ccgttaccat caattgtcgc gtcatttcga tcaatggctt
     8641 agtcaaagag cacaacagtt tttacataat aaaccaactt ctcaagatga tgactatctt
     8701 tattttgaga agacttcgac gagtcaagct tttcggatac aaaaaagtga ttgccagtta
     8761 ttgctgagat cacttgatga tttacctatc tacttaggcg aaagcaaatc aaaattcgga
     8821 atgaacacat tccatatttt tttactagaa aacgaaccca gatgggaaaa aagccagtct
     8881 tcgcctggtg gatacctgac aactaagatg agtacattag tcactggttt tttcctcatt
     8941 cttttagctg gaacaatcta ttatgggaga tacactcagc caaaaatgtc ttctcatata
     9001 aatgctgctt ctcacatgac aatgaactca actaacgata cgcatataca aaagacaaat
     9061 gaagggttgt tagctgatcc taagcaaaag gtaaatttga caacacaaaa gataaatgat
     9121 ctcaaacttg atacagagac gcactctctc tatatgacaa cagatagtgg tgctaattgg
     9181 gtatttgtgc cgattgattc gcaatggttg cgttttggcg aatacaccct gacaacgggg
     9241 ctgattcctg aaggttattg gatggacaac acatatgata tttccgccga tttctcatgg
     9301 tttatctatt cttctgggga tgatgtacgt ctgctgactt caagagataa tggtaaaaca
     9361 tggcaaacgt ctattatcac ccaatcagtt cagccgatac gttatcgaaa agcggtattt
     9421 tatggcaacg atggtctatt tatctgtaca tcagccagtg ggatgtctgc agaaactttg
     9481 tacatttaca ctactatgga tggcggaatc acttggataa aaagtggaag tacaatgatc
     9541 aatcagccag tacaaaatgt cagcttcgtt acaacttctt taggttttgt ttcgacacgt
     9601 gaaagtttgt tttatacaaa caacggagga cgctcattca aagaatcagt tctgtcgatt
     9661 ccggaaaact atcagacagg ggggatagat ctttttcaat caccaaatga agttactcaa
     9721 ataagcgcga atacgttaga gacaaaattt tatttgttga aaaaaggacc aatcgatgca
     9781 gggaaaatgt ttgcctgcct cttccgttca actgataacg gggaaacatg gcaattcgtt
     9841 cagcaactat cgcaagtaga acaaggagat taaagaagag agattgtgag cctgacgttc
     9901 agcaccgagt cacaagaaac aaattcaaaa aacagcttct catatttgtt gaatagtgta
     9961 actcgtgatt cggagttgac aagagcaaca agaagcgatt ttgaagtata gcttctcgta
    10021 ttttacaaga tcgtgtctta ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa
    10081 aagggattgt gacaagtctt ttgtcactac ttaccgaacg aaactgaagg agctatccaa
    10141 aaagtatcat tagtattatt ctttgtgttt ctagtggcac cactagtaca tttaaaaaag
    10201 ctatcgatca tggactaatc atacatgatc gatagctttt tagataatgt ttgttacgct
    10261 atctgaaatt gactattata aagactggca taaagcgttg gtgattggag tagttcagaa
    10321 tgtgttcctt tttcgacgat atcgccattc ttcattacaa ggatcatgtc tgcattttca
    10381 atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat cttttgtcac agcttccatt
    10441 gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag aagttgcttc atctaaaata
    10501 acgacaggag gattcgctag aatgactcga gcaattgtca ataattgttg ctgtccttga
    10561 gataacgtgc cgttttcgct agaaatgatc gtatcatatc cgtctggcag tgttcgaata
    10621 aagtgatcac attgtgcgat ttcagccgct tcgataattt gttctcttgt ggcatccatt
    10681 ttcccgtagg cgatattatc agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc
    10741 atcccaaaag aacgacgtag ttctccacgt gaaagcttgg taatatcaag accgtcgaat
    10801 tcgatctttc ctttattcag ttcataaaaa cgcatcagca gattgactaa agtcgttttt
    10861 cctgcaccag ttggaccaac gatcgctacc gtttgttttg gctttactga aaaatcgact
    10921 tgattcatca atattttatc tgatgtgtaa ccaaactgta catttttgaa agctattttt
    10981 cctttcggtt gttctaaaag tcttgcatct ggaaggtctg gaatttcatc tgtttcatct
    11041 aatatctcaa atatcctatc gattgctgcc attgcggatt ggatcgagtt gatcacataa
    11101 gaagcggtag aaattggttc agaaatctga ttgatgtact gaagataagc ttgtaagaag
    11161 ccgatcgtca caccacctga tagaacaagc atagcaccga ataccgcact tatgatgaag
    11221 gcgagttgat taatgaaacg aatggccgga tagatagcaa agttcataaa ttgagcacgt
    11281 ttaaaagcgt tgtattgctt tttatttact tcgtgtatag cagcttctgc atgagcttgc
    11341 tgattgaatg ttttgaccac tagattacca gctaagattt cttccattcg attatttaaa
    11401 acacccagtt catactgatt ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt
    11461 gtaacaattg aactgccaac gatcagcaat aagacgagca aagtaagctt gctttcgata
    11521 taaaacagca tcagaccagc agcagctatt gttacgacag aagtaaaaaa ttgattgata
    11581 cctgtcaata acacttgaga aagttgattg agaccggtag ttgtccgact aatgatgtca
    11641 cctacttgat gattgtcaaa aaacgacatc ggtaatgttt tgaatttttt tgtgatttct
    11701 tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca ttgtccgttc ctgaataaat
    11761 gaagtgatgc tgcttaagac tgaacagccg atcagcaata agacagggaa caacaatgct
    11821 tgtttgatat cactgaaaga aagctgtcca atacctgttt ttcttatttc tgataagaga
    11881 tcatcgatcc cgattcccat gatcaagggc atggctacga ctaataggtt cccgaataaa
    11941 ctgcatagaa acaatccgta aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt
    12001 atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg tatcccttct ttctatattc
    12061 cttgtgattt tgcaaactcc tgataagttt ggcaattaga cagtagttct tcatgagtac
    12121 cttgtccaac gatttttcct tcatctaaaa caatgatgtt atctgcttgt tggattgtac
    12181 tgaggcgttg agcaacaatc acgagagttt tctccgacat gtgtttatga agggctgctc
    12241 ttaatgctgc atctgtttta tagtctaaag cggagaaact atcatcgaat acatagatct
    12301 ctgctggttt gatcaacgct cgagcaatac atatccgctg tttctggccc cctgaataat
    12361 tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg aagggtgtcc actagttctt
    12421 ctaattgagc gatcttgatc gctttagaca actcttctgt tgaggcatat tcatttccca
    12481 tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc cttctgaggg acataactga
    12541 tgtgggagcg gatctgttct tgagtcattt gagtaagagg aataccacca agggttactt
    12601 ttcctatcgt agggtcacta agtctcaaga gaagtttcgc caaactactt tttccagatc
    12661 cggtcccgcc aacgatcgct gttgtttttc ctttaggtat tgtgaaatca atatcttcta
    12721 gaactggatc agagatttca gtatatttaa atgtgacatg ttcaaaagca gctgcatttg
    12781 tctgatcaaa ggttggttca gctactgttt tgatcgtctc ttcctcaatt tcgatctctg
    12841 cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga tttagggata atgaccaata
    12901 ccatagctgc gatcatcaag taagcaagtg tcagcataga gtattcgact acagcagtca
    12961 cgacaccgat ttgtaatgct ccttctgcta caagatttcc gccaaaccac aaaatagaag
    13021 cataaacgat tcccatgagc aaaaaagcaa ttggagtgac ccaagcaaat attcggttca
    13081 ctttgatcat ttgattagca tagtcagcaa agctcttatt cgttcgcttt tcttcatatg
    13141 attgattatc aaaggcacgg atcatattaa tcccagtaaa gaattctctc aatgtgatgg
    13201 tgatccgatc catttttggc tggatactca aagataaagg tgcccctcgt ttcatcagtc
    13261 gataaacaat ccatacataa aaaacaacag agatcaatgt aacccatgtt agtaatagag
    13321 aataacgaaa catcatgaga atggtgaaaa cgcaaataac cggtgctggt aagatcattt
    13381 ggcaaaataa aacaatcatt tgctggacat tatcgacatc attcgtcatt cttgtcaaaa
    13441 gagatccaga actaaaacga ctgatatcct tcattgacag ctgttggaat ttacggaaat
    13501 atttctcccg cgtttggaat ccaaacctag cggaagccaa tgctgaaaag taactagagg
    13561 caactgcagc gattgttcct agaaacgcta caagcagcat ttcaactcca atttttttta
    13621 taactgaggc ttcgccactt gctatacctg tgtcgatcag ctttgctact aattttggta
    13681 cacctaatgt accggcgatt tgtaaacaga tcatcacaaa ggtaaggaga gcgagtcctt
    13741 tgtttttctg taagaatgtt tttaagatag acatattatt cctccttttt tcctgatact
    13801 tttcgattct ataccttcaa gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa
    13861 gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa gtatcggaac tatttgattt
    13921 gccaaaatca acgatccgct attgggatga acaaggatta atccgttcaa gtagacaaga
    13981 agagaatgat tatcgattgt ttgatatcga tgatattttt atgctttatg atatcgtttt
    14041 ttatagaaaa ttagatatac cgatcaaaca gatgaagaat ttatatggaa aaacattgac
    14101 ggagttatat gagacacttg atgaaacaga aagaagaatc cataaagaac tggtggtgat
    14161 gaaacaaaaa caaaaagaga taagggagca aaaaaaacag ttgaaattga tgatcgatac
    14221 aaacgaggaa gaatttcctg tagaagagat cccttttgac tgtatgatct caacggagtt
    14281 tgaagatatt gtggaaatca aaaaatttct acctaactat tcaagctttg ggatgatgag
    14341 tatgccagct tccagttcac agacgatgta tggatttttt atcgacccta gtgaagtaca
    14401 tttatttact caagacgtga tttgggagaa aaaagatacg gctgtttatc gtcgattctt
    14461 attgaaatcc gagatgaacc atgcggaacg taataatatt ttagaaatca gagaaagaat
    14521 gtatgaaaaa ggatggaaaa caggagaagt gatcggccag tatctattga cgaatacgga
    14581 tgaaaacaat atcagaactg aatattatca tgcatggata gaaatgaaaa aataggaaat
    14641 atgtgtttaa gataactcga aaatgatggt tgttttagtt ataaagaaaa attttctggt
    14701 tcgattacgg gtttaatgtg ggggattatt agtcgtggat agatagaatt ttagtttttt
    14761 agaggaaata tgtctttaaa aatggaatgg ttgcaattct tagcgatata tacgatgtaa
    14821 ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg tgttatgtat aattcatggt
    14881 gaactgagac taatgagaag aaaaattcat attgttatga gtgagattgt atcagatgag
    14941 aatgataaat taaataggtg cataaaagca ttacacttaa aatacattgt tgcttacatt
    15001 attacaatat gttaataagg caaaaataaa tagatggatt gaataaacat attttttaat
    15061 caaatagtcc ctactgtgac tttcctcagc tatcggtgat actaccgata gcattttttt
    15121 gcttataaaa taattataag tatttagaga tttttcttct aacattcttc aaacaagaaa
    15181 aatcacaaag gttattgtta tttttatata ttctttttaa gaatgattgt ttttttatag
    15241 aaattttaaa aaataaaaca cgttattgat ggatagttag tttgttattt ggtaatatta
    15301 tagaagttga ctggtagtga tttatttttc gtcgttttat aaaaatgtta ttttttggaa
    15361 aaattcacaa atatgtaacg aatgtttttc tggaatttgc tgtgttaaaa agcagtatga
    15421 ctaatataaa tactattgtc atttatgcaa ttttttatgg atagtaattt tagaggggaa
    15481 tcaatgaagg aagaatagaa gggaaaaata gttgcaaata cgccagttag caaaaatagc
    15541 aatacatctt caagctttaa aattcgagag aatcatcttg gtagtggaat caactacaac
    15601 aaaaggatta aaccaattaa tagatagaat taccaatgga aaaatagaaa aggtagttat
    15661 tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt agtgaaaccc tttgcaatat
    15721 atggtacaga atatagaaat tattgacatt acagaaaatg atgaagattt aatacagatt
    15781 gtcactattt gtagatacct tttacaagga aaccgagcca ataaagctaa gaagatgatt
    15841 aaggagttga tggaagacga tacaagttaa gaaagtgaaa ctacgcccaa ataaagagca
    15901 aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca tcgattcggt ggacagttta
    15961 ttagtaatga cggtttgaga acatccatga cgagaatgaa ggaacatcct aaatgcgttt
    16021 agttgaacca agtgccaaat aatatggcca aataagcgat cagttatcga tgaatcccaa
    16081 gtattctaaa ctatatattc attttgatgc taagcattga tatatttttt tgggattgat
    16141 gaaggttaac caatacaggg actcactgat gaaattctag agtctgacct tggtatcaac
    16201 gatttagtga ttgtttctaa tggaaaagta taaaagtata taagtacatt aacaaaataa
    16261 ctattagggt tacgctccag cttgtgcaag gcactggcag gaggaatcgt gaaatcataa
    16321 ttatggttaa gccacacgga caactaaaag tcatacaaag ctatcgaatc aatcaacaga
    16381 agacaatcgg caatcattag ctttgtttac agcttacatt gcaacgcttg agttgaaccg
    16441 aaaatacgag ggaggtaacg cagccatttt gagttgggga atctttttag aaaattatcg
    16501 ggtaccttgt tgtttagtca aaattataag ggggagtaat agtgaggaac aaatttcata
    16561 aactgttaag tatgtttcta gttatgctta ttgtgatcaa tcaatttggg aacataacgg
    16621 ttctagctga gtctttagca gaacagccag agcgttcgct gacgatcgaa gttgatcaag
    16681 aggaggctgc tttgggtgag cagttccagt tgcgagtcag aggaacacca gacctgctaa
    16741 atgcattggt cattgataag agtgaggatt tgattgaagg ggaagtgaag caactcaatg
    16801 attacgaacg tctgattact tattcttccc agcattcagg taaatacgaa ttgaccgcaa
    16861 gaagcggtga tattcaatca aatacggtct caatagaaat cagtgtacca gaagaaagca
    16921 caagtgagtc ttttaatgta ccagaagaaa gcacaagtga atcttttaat ataccagaag
    16981 aaagcttaag tgaatctttt aatgaagcca gatcatctga gccatctata acgcaggaat
    17041 ctcagattga aagacaagag gagctcttga attatgacat cggcatctct gctgagcgcc
    17101 ccactttgcc gagcaggcat acacaaacgt taaagggata cattcaagga ggcgctggcg
    17161 atattcttga agaatatggg gagatggtcg tacgagttcc tagagcatcg attggcttaa
    17221 ttgagttcat tagagtggag gtcgctgatg attcaccttt aaagccactg gcattttacc
    17281 aagaaggcac agacgttgtt tttccattta cctttgatta tgaaggatta aatgaagccg
    17341 ctcagtcgta tacattcagt atcacgtttg aagttgcttt ctttcaatca ggcggtactg
    17401 agccaaataa tgactttcaa gtgagtctta cttatgctga gcgagcagca aacacggaat
    17461 tttcaattac gcctattcct tcatcaagac ccattttttc gaaattatac tatggctcta
    17521 gagaaatcga cagccaagga aatcttggga atctgctgat tgacgggcaa gacgtgacca
    17581 acagtaataa atttattttg caggtaaatt atgaaggatc acgaaaagac ggtgtagtgg
    17641 ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa tccgactaca tggagttcca
    17701 ctggaaatag agaacctatt tcaggtgggg ttcggatcgc agaagtaacg aatccaggtg
    17761 aagcaaacgt tagaactcat tatgtttccg aggaatttgt ggacaaagta tcttttaata
    17821 aggaaacgaa tacacttgtt gtggactttg gtaatctagc agagggtcaa tcgtatttta
    17881 ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa tgaaggaaga caagaggatg
    17941 ggttttctcg gaatcgagcg agtatgacct acaataatca ggtaatgagc cgagactacc
    18001 gcttgagaat ccctgaaagt aatcttccgg caatctcttt aagaaaaagt gtggacaaaa
    18061 atgttatcaa tctcaatgaa aatgaactaa cgtatactct gcatgtctcg atgttttctg
    18121 gaacaagaaa tacgcttgtt attgaagatg ttttggaaga aggcatggaa tttgtgtcat
    18181 ttctatcaga tattccctct ttcattgatg ccgaagtcaa taatggaata gttcgtttca
    18241 gtgcgaataa tacgattggt ggtccgacaa gttttaccct ctcttttaag gtcaatgtag
    18301 aggcgtatgc ggtcggcgat caaattttaa actttgcaac gcttgtgaat agtgacaatc
    18361 gattgaatac gagtactgta agaacaagga aaatcgatgg gcgtattaaa gtaattaaaa
    18421 ctgatgagaa tggccaaagg ctggcaggag caaaatttga aattttatca agcgattcac
    18481 aagtgataca agaaggtata acggcagaaa acggagaatt tttaagtgaa ccgcttcctg
    18541 taggtagcta tcaagtccga gaaacagaag caccggaagg gtatctttta gatgcgacgg
    18601 tacatgatgt ttttataaat gaatactcca tagaagaagt gacgttaacc attgaaaaca
    18661 tccttgatac gggtggtgtt gaactaatta aacatgctga aggcagtggt gaagtccttc
    18721 aaggagctgt ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggt
//
LOCUS       DAFFQY010000053        17730 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFFQY010000053 DAFFQY010000000
VERSION     DAFFQY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.053"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            352..1041
                     /locus_tag="LRB02_001731"
     CDS             352..1041
                     /locus_tag="LRB02_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4659927.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="LRB02_001732"
     CDS             1028..2236
                     /locus_tag="LRB02_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4659928.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="LRB02_001733"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="LRB02_001733"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL4659929.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LRB02_001734"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="LRB02_001734"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL4659930.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="LRB02_001735"
     CDS             complement(6339..7034)
                     /locus_tag="LRB02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL4659931.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LRB02_001736"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="LRB02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL4659932.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="LRB02_001737"
     CDS             complement(8233..8424)
                     /locus_tag="LRB02_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL4659933.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="LRB02_001738"
     CDS             complement(8445..9335)
                     /locus_tag="LRB02_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL4659934.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="LRB02_001739"
     CDS             complement(9322..10089)
                     /locus_tag="LRB02_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4659935.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LRB02_001740"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="LRB02_001740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL4659936.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LRB02_001741"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="LRB02_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL4659937.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGTNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LRB02_001742"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="LRB02_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL4659938.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="LRB02_001743"
     CDS             14660..15043
                     /locus_tag="LRB02_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659939.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="LRB02_001744"
     CDS             complement(15211..15969)
                     /locus_tag="LRB02_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL4659940.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="LRB02_001745"
     CDS             complement(15987..16805)
                     /locus_tag="LRB02_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL4659941.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LRB02_001746"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="LRB02_001746"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL4659942.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LRB02_001747"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="LRB02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL4659943.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         4955 a         3685 c         3237 g         5853 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtgtcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg acttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 gtacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat
//
LOCUS       DAFFQY010000054        17687 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFFQY010000054 DAFFQY010000000
VERSION     DAFFQY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.054"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(136..1716)
                     /locus_tag="LRB02_001748"
     CDS             complement(136..1716)
                     /locus_tag="LRB02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL4659944.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="LRB02_001749"
     CDS             complement(2117..3493)
                     /locus_tag="LRB02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL4659945.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="LRB02_001750"
     CDS             complement(3621..4739)
                     /locus_tag="LRB02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4659946.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="LRB02_001751"
     CDS             4881..5315
                     /locus_tag="LRB02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4659947.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="LRB02_001752"
     CDS             complement(5393..5926)
                     /locus_tag="LRB02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL4659948.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LRB02_001753"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="LRB02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL4659949.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LRB02_001754"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="LRB02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL4659950.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="LRB02_001755"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="LRB02_001755"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL4659951.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="LRB02_001756"
     CDS             complement(9651..10229)
                     /locus_tag="LRB02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL4659952.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="LRB02_001757"
     CDS             10551..11021
                     /locus_tag="LRB02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL4659953.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="LRB02_001758"
     CDS             complement(11129..12355)
                     /locus_tag="LRB02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4659954.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="LRB02_001759"
     CDS             complement(13109..13309)
                     /locus_tag="LRB02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4659955.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="LRB02_001760"
     CDS             complement(13443..14051)
                     /locus_tag="LRB02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL4659956.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="LRB02_001761"
     CDS             complement(14136..14606)
                     /locus_tag="LRB02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL4659957.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="LRB02_001762"
     CDS             complement(14654..15568)
                     /locus_tag="LRB02_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL4659958.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="LRB02_001763"
     CDS             15714..16517
                     /locus_tag="LRB02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL4659959.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="LRB02_001764"
     CDS             16695..17642
                     /locus_tag="LRB02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL4659960.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5363 a         3542 c         2911 g         5871 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagtt tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DAFFQY010000055        17516 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFFQY010000055 DAFFQY010000000
VERSION     DAFFQY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.055"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            100..2427
                     /locus_tag="LRB02_001765"
     CDS             100..2427
                     /locus_tag="LRB02_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4659961.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="LRB02_001766"
     CDS             2553..2882
                     /locus_tag="LRB02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL4659962.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="LRB02_001767"
     CDS             2922..3926
                     /locus_tag="LRB02_001767"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4659963.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="LRB02_001768"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="LRB02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="LRB02_001769"
     CDS             5558..6730
                     /locus_tag="LRB02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4659964.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="LRB02_001770"
     CDS             complement(6892..7347)
                     /locus_tag="LRB02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659965.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="LRB02_001771"
     CDS             7936..8358
                     /locus_tag="LRB02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4659966.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="LRB02_001772"
     CDS             complement(8524..9717)
                     /locus_tag="LRB02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4659967.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="LRB02_001773"
     CDS             9941..10318
                     /locus_tag="LRB02_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4659968.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="LRB02_001774"
     CDS             10306..10644
                     /locus_tag="LRB02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4659969.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="LRB02_001775"
     CDS             10778..11221
                     /locus_tag="LRB02_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL4659970.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="LRB02_001776"
     CDS             11483..12298
                     /locus_tag="LRB02_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4659971.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="LRB02_001777"
     CDS             12308..12970
                     /locus_tag="LRB02_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4659972.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="LRB02_001778"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="LRB02_001778"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL4659973.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="LRB02_001779"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="LRB02_001779"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL4659974.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="LRB02_001780"
     CDS             15692..16189
                     /locus_tag="LRB02_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL4659975.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="LRB02_001781"
     CDS             16374..16634
                     /locus_tag="LRB02_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659976.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="LRB02_001782"
     CDS             complement(16996..17235)
                     /locus_tag="LRB02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659977.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DAFFQY010000056        17081 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFFQY010000056 DAFFQY010000000
VERSION     DAFFQY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.056"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(17..538)
                     /locus_tag="LRB02_001783"
     CDS             complement(17..538)
                     /locus_tag="LRB02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL4659978.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="LRB02_001784"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="LRB02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL4659979.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="LRB02_001785"
     CDS             4618..5505
                     /locus_tag="LRB02_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL4659980.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="LRB02_001786"
     CDS             5502..6671
                     /locus_tag="LRB02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659981.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="LRB02_001787"
     CDS             6695..8050
                     /locus_tag="LRB02_001787"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL4659982.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="LRB02_001788"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="LRB02_001788"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL4659983.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="LRB02_001789"
     CDS             10336..10626
                     /locus_tag="LRB02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659984.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="LRB02_001790"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="LRB02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL4659985.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="LRB02_001791"
     CDS             11667..12326
                     /locus_tag="LRB02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4659986.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="LRB02_001792"
     CDS             12483..13415
                     /locus_tag="LRB02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4659987.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="LRB02_001793"
     CDS             13569..14858
                     /locus_tag="LRB02_001793"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL4659988.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="LRB02_001794"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="LRB02_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL4659989.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="LRB02_001795"
     CDS             16438..17025
                     /locus_tag="LRB02_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL4659990.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5587 a         3065 c         3740 g         4689 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag t
//
LOCUS       DAFFQY010000057        16676 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFFQY010000057 DAFFQY010000000
VERSION     DAFFQY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.057"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            38..1372
                     /gene="dnaA"
                     /locus_tag="LRB02_001796"
     CDS             38..1372
                     /gene="dnaA"
                     /locus_tag="LRB02_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL4659991.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            1570..2700
                     /gene="dnaN"
                     /locus_tag="LRB02_001797"
     CDS             1570..2700
                     /gene="dnaN"
                     /locus_tag="LRB02_001797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL4659992.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            2929..3171
                     /gene="yaaA"
                     /locus_tag="LRB02_001798"
     CDS             2929..3171
                     /gene="yaaA"
                     /locus_tag="LRB02_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL4659993.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            3158..4282
                     /gene="recF"
                     /locus_tag="LRB02_001799"
     CDS             3158..4282
                     /gene="recF"
                     /locus_tag="LRB02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL4659994.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            4279..6225
                     /gene="gyrB"
                     /locus_tag="LRB02_001800"
     CDS             4279..6225
                     /gene="gyrB"
                     /locus_tag="LRB02_001800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL4659995.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            6245..8716
                     /gene="gyrA"
                     /locus_tag="LRB02_001801"
     CDS             6245..8716
                     /gene="gyrA"
                     /locus_tag="LRB02_001801"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL4659996.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            8952..9251
                     /locus_tag="LRB02_001802"
     CDS             8952..9251
                     /locus_tag="LRB02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL4659997.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            9301..9813
                     /gene="ssb"
                     /locus_tag="LRB02_001803"
     CDS             9301..9813
                     /gene="ssb"
                     /locus_tag="LRB02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4659998.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            9839..10075
                     /locus_tag="LRB02_001804"
     CDS             9839..10075
                     /locus_tag="LRB02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL4659999.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            10218..12191
                     /locus_tag="LRB02_001805"
     CDS             10218..12191
                     /locus_tag="LRB02_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL4660000.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            12197..12649
                     /gene="rplI"
                     /locus_tag="LRB02_001806"
     CDS             12197..12649
                     /gene="rplI"
                     /locus_tag="LRB02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL4660001.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            12923..14290
                     /gene="dnaB"
                     /locus_tag="LRB02_001807"
     CDS             12923..14290
                     /gene="dnaB"
                     /locus_tag="LRB02_001807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL4660002.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            15070..16362
                     /locus_tag="LRB02_001808"
     CDS             15070..16362
                     /locus_tag="LRB02_001808"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL4660003.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         5549 a         3028 c         3575 g         4524 t
ORIGIN      
        1 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
       61 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
      121 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
      181 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
      241 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
      301 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
      361 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
      421 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
      481 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
      541 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
      601 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
      661 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
      721 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
      781 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
      841 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
      901 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
      961 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
     1021 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
     1081 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
     1141 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
     1201 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
     1261 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
     1321 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
     1381 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
     1441 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
     1501 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
     1561 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
     1621 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
     1681 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
     1741 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
     1801 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
     1861 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
     1921 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
     1981 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
     2041 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
     2101 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
     2161 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
     2221 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
     2281 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
     2341 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
     2401 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
     2461 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
     2521 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
     2581 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
     2641 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
     2701 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
     2761 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
     2821 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa
     2881 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
     2941 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
     3001 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
     3061 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
     3121 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
     3181 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
     3241 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
     3301 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
     3361 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
     3421 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
     3481 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
     3541 gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
     3601 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
     3661 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
     3721 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
     3781 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
     3841 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
     3901 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
     3961 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
     4021 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
     4081 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
     4141 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
     4201 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
     4261 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
     4321 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
     4381 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
     4441 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
     4501 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
     4561 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
     4621 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt aaacgcgctt
     4681 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
     4741 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
     4801 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
     4861 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
     4921 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gatcaaaagt
     4981 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
     5041 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
     5101 aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca tgaatcagga
     5161 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
     5221 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
     5281 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
     5341 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
     5401 ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
     5461 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
     5521 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
     5581 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
     5641 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
     5701 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
     5761 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
     5821 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
     5881 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
     5941 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
     6001 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
     6061 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
     6121 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
     6181 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
     6241 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
     6301 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
     6361 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
     6421 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
     6481 tcctcatgga gaccgtgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
     6541 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
     6601 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
     6661 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
     6721 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
     6781 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
     6841 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
     6901 aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
     6961 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
     7021 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
     7081 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
     7141 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta tcttgaataa
     7201 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
     7261 aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg aacatcaacg
     7321 cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa gagcacacat
     7381 cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
     7441 gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt tatccgatcg
     7501 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
     7561 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
     7621 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
     7681 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
     7741 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
     7801 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
     7861 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
     7921 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
     7981 tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg aaagcatcca
     8041 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
     8101 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
     8161 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
     8221 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
     8281 ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg aggaagatta
     8341 tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca cagaaaaagg
     8401 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
     8461 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
     8521 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
     8581 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
     8641 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
     8701 aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata accgattaaa
     8761 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
     8821 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct tgtgagtagt
     8881 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
     8941 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
     9001 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
     9061 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
     9121 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
     9181 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
     9241 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
     9301 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
     9361 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
     9421 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
     9481 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
     9541 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
     9601 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
     9661 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
     9721 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
     9781 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
     9841 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
     9901 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
     9961 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
    10021 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
    10081 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
    10141 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
    10201 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
    10261 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
    10321 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
    10381 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
    10441 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
    10501 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
    10561 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
    10621 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
    10681 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
    10741 ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa gagatttctt
    10801 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
    10861 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
    10921 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
    10981 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
    11041 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
    11101 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
    11161 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
    11221 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
    11281 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaatgaga
    11341 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
    11401 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
    11461 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
    11521 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
    11581 tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
    11641 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
    11701 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
    11761 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
    11821 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
    11881 ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag
    11941 cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag
    12001 acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg
    12061 aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa aatacgacag
    12121 ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat caaatatatg
    12181 ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa
    12241 aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt
    12301 ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga
    12361 aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga
    12421 agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat
    12481 cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg
    12541 aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct
    12601 tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat
    12661 agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga
    12721 gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt
    12781 cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt
    12841 tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa
    12901 ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa
    12961 tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga
    13021 tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga
    13081 gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg
    13141 gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc
    13201 attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc
    13261 attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat tcgagcaagg
    13321 agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa
    13381 aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat
    13441 cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct
    13501 ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc
    13561 ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg
    13621 ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg
    13681 tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg
    13741 gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac
    13801 accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg
    13861 caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa
    13921 ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa
    13981 agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg
    14041 accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc
    14101 cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga
    14161 gccaaaaaat gacaacatca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg
    14221 aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga
    14281 agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg
    14341 caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg
    14401 tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc agctgaccct
    14461 tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg tttctctaat
    14521 actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc tcctgccaac
    14581 tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga aattgctcct
    14641 tgtgagtcag agttaaacgg caggctcaca acctctttct taaacggtgt tcaaaagctt
    14701 gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt tgtttggctg
    14761 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccaaaag
    14821 tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat tttcgtacat
    14881 tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa taaccattct
    14941 aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa tcattcgatt
    15001 ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa tatagaacga
    15061 ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga agggaaagga
    15121 aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca aggtggcgac
    15181 aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt gattccttct
    15241 gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt caatcctaaa
    15301 tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag taatttgcgt
    15361 atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca attacaagaa
    15421 gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc tgcctatatg
    15481 gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga aatttttgaa
    15541 gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat gtttgattca
    15601 gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca acaaatcaaa
    15661 caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg caaacgcgtg
    15721 ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta tccttttgtt
    15781 acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg gccttcaaaa
    15841 atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga tggaccattt
    15901 ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg agaatacggt
    15961 acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat gcgccattct
    16021 aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt aagtggattg
    16081 gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta ccactatcca
    16141 gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc aggctggtca
    16201 gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg taactatgta
    16261 cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg acctgataga
    16321 acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa acgacgactg
    16381 aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa taataacttg
    16441 aagaggtagt gaaagagacg tttagcctta ttgccgatag gctgtctctt gaacactgtt
    16501 tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaacaaacgg
    16561 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    16621 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tctcca
//
LOCUS       DAFFQY010000058        16630 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFFQY010000058 DAFFQY010000000
VERSION     DAFFQY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.058"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            5..2377
                     /locus_tag="LRB02_001809"
     CDS             5..2377
                     /locus_tag="LRB02_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660004.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="LRB02_001810"
     CDS             2438..3898
                     /locus_tag="LRB02_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL4660005.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /locus_tag="LRB02_001811"
     CDS             3909..6113
                     /locus_tag="LRB02_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBL4660006.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="LRB02_001812"
     CDS             6129..6278
                     /locus_tag="LRB02_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660007.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6354..6947
                     /locus_tag="LRB02_001813"
     CDS             6354..6947
                     /locus_tag="LRB02_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL4660008.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6960..7859
                     /locus_tag="LRB02_001814"
     CDS             6960..7859
                     /locus_tag="LRB02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL4660009.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7862..8335
                     /gene="ssb"
                     /locus_tag="LRB02_001815"
     CDS             7862..8335
                     /gene="ssb"
                     /locus_tag="LRB02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4660010.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            8701..8961
                     /locus_tag="LRB02_001816"
     CDS             8701..8961
                     /locus_tag="LRB02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660011.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            9406..9612
                     /locus_tag="LRB02_001817"
     CDS             9406..9612
                     /locus_tag="LRB02_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL4660012.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9612..9863
                     /locus_tag="LRB02_001818"
     CDS             9612..9863
                     /locus_tag="LRB02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660013.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            9879..10295
                     /locus_tag="LRB02_001819"
     CDS             9879..10295
                     /locus_tag="LRB02_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660014.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            10277..10549
                     /locus_tag="LRB02_001820"
     CDS             10277..10549
                     /locus_tag="LRB02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660015.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            10716..11117
                     /gene="tnpA"
                     /locus_tag="LRB02_001821"
     CDS             10716..11117
                     /gene="tnpA"
                     /locus_tag="LRB02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL4660016.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            11129..12274
                     /locus_tag="LRB02_001822"
     CDS             11129..12274
                     /locus_tag="LRB02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660017.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            12512..12775
                     /locus_tag="LRB02_001823"
     CDS             12512..12775
                     /locus_tag="LRB02_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL4660018.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12769..13119
                     /locus_tag="LRB02_001824"
     CDS             12769..13119
                     /locus_tag="LRB02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL4660019.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(13143..13757)
                     /locus_tag="LRB02_001825"
     CDS             complement(13143..13757)
                     /locus_tag="LRB02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4660020.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            14207..15532
                     /locus_tag="LRB02_001826"
     CDS             14207..15532
                     /locus_tag="LRB02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL4660021.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            15525..15875
                     /locus_tag="LRB02_001827"
     CDS             15525..15875
                     /locus_tag="LRB02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660022.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            15872..16051
                     /locus_tag="LRB02_001828"
     CDS             15872..16051
                     /locus_tag="LRB02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660023.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            16187..16477
                     /locus_tag="LRB02_001829"
     CDS             16187..16477
                     /locus_tag="LRB02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL4660024.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         6154 a         2694 c         3344 g         4438 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaac atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaat gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagttaaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctaaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtttcga tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaagtcattt atgaagaagt cgcaaaagaa gaaaagatca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaat tggtttccgc
     3541 tcacaaagta aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagctat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagcttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggatt ggaatgatca attgaagcat gaaatctacc aacaagagat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggtaga cagcaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaaaccgtc acaagcgaag gcttatgccg
     3961 atagcttttc taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgtttt
     4021 caggagaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatagctt
     4261 ggacgatcat tcaacaagca ggcgccgatc atggaaagat atttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagat gcagaagaga
     4381 cctacccaaa atttgaagaa gcacaagcac gccagatcgc cgattggttg atcggcatga
     4441 acggtagccc gctgtatagt ttactcttgc agcaaaaagg cataccagga agtttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attacaggaa aagattagaa
     4561 acttccaaaa agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggtac gatccaccaa gttgaaaccg
     4741 agaaaaaaag tgcggcaagt ccacgtcttt tttctctatc cagccttcaa tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag
     4861 ggaaatatct aagctaccca cgaacagata ccccttatat tacagaaggg gagtatgcgt
     4921 atctgttgga tcacttagac gaatacaaac actttttaaa ggccgaagcc attcctactc
     4981 ctattcatac accgaatagt cgatatgtga ataataagaa agtccaggaa cattacgcga
     5041 tcattcctac aaagacggtc atgacggcag cggcttttga acaattaagt ccactgcagc
     5101 aggctattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aagtatacct
     5161 atgaagaaac aacgatcttg acgcaagttc agcagcttca gctaaagaca gtcggaaaag
     5221 taccagtaga tctaggttgg aaaaagctgt ttggcaaaga gagcgacaga aaagaaaagg
     5281 aagaagaacc gctacttcct aaagtgacaa aaggagaagc ggtgacggta gatctacaag
     5341 tccttgaaaa agaaacgaag ccaccacaac cttataccga aggtacgttg atcacagcga
     5401 tgaaaacagc tggaaaaacc gtggatagcg aagaagcaca atccatattg aaagaagtag
     5461 aaggaatcgg tacagaagca acacgagcaa atattattga aactttgaaa caaaaggaat
     5521 atatcaaggt agaaaaaaat aagcttgtgg tgacaaataa agggatttta ctttgccaag
     5581 ccgtggaaaa agagccgctg ctaacaagtg ccgaaatgac cgccaaatgg gaaagttact
     5641 tattaaagat tggtgagcag aaaggaacac aagctacttt cttagctaat atccaaaaat
     5701 ttgtctctca cttgttggaa gtcgtaccgg gacaaatcca gtcaacagat tttggatcaa
     5761 cgttacaaga ggtgaaagca gccagtgaaa aacaggaagc aacgcgtcat ttaggtgttt
     5821 gtccaaaatg tcgggaacaa gaagtcttgc tttatcaaaa tgtggcggca tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gccacacaac
     5941 tgaaagagat cattcaaaac ggccgaacaa gccaatcagt cagaggacta aaaggtcaga
     6001 aaggttcgtt tgaagcaacg atcgttttaa aagaagactt tacaacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac tataagaaaa gaacacgccg cactacaaaa taatgaaggg
     6121 gttatcctat gcgaaataca acacacaaac aacaagaaat cgaaaatcaa aaagcaaaaa
     6181 ttcgtttaga acaagagaaa ttacgtgcaa ataaaagaag actgcaacaa ttaaagaacc
     6241 agcagcgcat tcaaaaagaa atcgaattag atcgatagac gttaatcttg ttacaaataa
     6301 actactatga tatatttgat gtagtgtatt tgtaacgaag ggaggggaag taaatgcaag
     6361 ataaactgaa agaattgttg gctattcgcg atggaaattt atctatgaag gaagccagga
     6421 aatttggaat cgcagcaacc actgtacaaa gattggtgaa tcaaggaaaa ttgatcaaag
     6481 ttgatcgtgg ttattatgta gaggatggtc atggaataga tgatttattt tggcttcagc
     6541 aacgattttc caggggaatc ttctctcatg aaacgacttt agatatttac cagctttcta
     6601 cgaatatgcc gaaattcatt cacttaactt ttccccatgg ctataacgtt gatcgttcaa
     6661 ttacaaaaga gcagcagtta caatttcatt tcgtgaagaa agaagtttat gaactaggaa
     6721 aagtagaggg aacttccttt caaggaaatc ctattactat gtatgataaa gagcgtacac
     6781 tttgtgatat ttggaatccg agatatgaat ttgaatatga aatgaaatta gaagcattaa
     6841 aagagtacat ggaagaccca ttgagagatc caagtaaatt aagagattac atgacaaaac
     6901 tacatgtaga aaaagaaatg aaataccaca tgcaaagctt atattgatgg aggaaaaaga
     6961 tgacaccgac acaatttgca gcaaaaacaa gaaatttagc aaagagtaca ggattaaatg
     7021 cacagttagt gcaacggcat tttatgatgg atcgtctaat tgaacaaatt tctgaatcaa
     7081 agtacaaaaa tgacttcatt ttgaaaggcg gctttttatt aggatcaaaa tatggaatcg
     7141 atagaaggac aacaattgat atcgatacga catttagaaa tagcaaatta actgaagaaa
     7201 aattgaagag catttttaat gatttaacta gaaaacccac aaaagaaggc attgttttct
     7261 ctattcaagg aatgaaggaa acaagagaag ccgattttta tcctggattt caaattaaat
     7321 tggtggctaa tttagagaaa gtacgtgtac cgtttaaaat ggacgtgaca acaggagatt
     7381 ctatttatcc tgaagtagat acacactatc accaattaat gtttgaagat aaaaaagtgg
     7441 agattccagc ttatccaaca gaacaaatta ttgcagaaaa gctaagtgct acgttcagct
     7501 ttggcacaga taattcgaga gcaaaagatt actacgactt atttaccatt ccgaaactag
     7561 aaaatatcga tacgaaagag ttatacaaaa gtgttcgaaa tacatttaca aaacgtgggg
     7621 atacaaaacc gttgtccttt tattatgaaa gagaaatgga taagatcaga ggaaatgact
     7681 atttggaaaa gcagtgggaa aaatatcgct cagttaacac gtttgcgaat tccatttcat
     7741 ttacagatac agtcaacgaa attgatcacc taatgaaaag tgtgatcaaa attgaaaatc
     7801 aagaaagaaa acggcagttt aaaagatcac aacaaatgaa tcaagaaatg gagagataaa
     7861 gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcccgatt tacgttatac
     7921 tgcaagtggc acagcagtag cgacttttac cctagccgtc aaccgaaatt tcgcgaatca
     7981 aaacggcaat cgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac
     8041 aatggcgaca ttagctaaaa aaggcggctt gatcggtgtt gtcggacgga ttcaaacacg
     8101 aacttatgac aaccagcaag gacaacgtgt gtatgtgact gaagttgtag cagataactt
     8161 tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc aatcaaccac
     8221 cagtccaagc cccactacct ttgaacaacg ggatacaaac aatggattgg acgcttcaca
     8281 aaatccattt ggtggacaat caatcgatat tagcgatgat gatctaccat tctaagtagc
     8341 attttctatt ggttgcattt gctacggttt atgttataat ttagtcaaca aaaagaattt
     8401 tcaatctata accaaaaacc actagctggc gggctagtgg ttttctttta cgtttctatt
     8461 ataagaatta acgggtaggc ttactgtctt tcttggcaaa tttacaatca agccatttgc
     8521 agatatagtg cgcggctact tctgccatga ttgcaaaaac aaaatcaaaa agaattttca
     8581 aacctataac ctcctcccct tgccagatta ggccggggta gcagacagca agactattat
     8641 agcataattg aaatattcaa atgattggat ttttgctata attttagcga ggtgattgtg
     8701 atgaacaaat atgttggttt gcttgataaa atccgagtaa taaaaacgca accgttattg
     8761 gttcgcttta ctttacaaac gattcatgaa tcgattaatt gtgtagttgc agatattgaa
     8821 attatcgata aattgctaat tatggatgat ggaaaataca atattgccgt cacaggtcac
     8881 ttcaataaac gaaaccagct agttattgct agcatgtatg ttagaaatcc cgatcagttt
     8941 acgcgattga tgggcatata aaaaaagaag agttgcaatc ctaatgtagg actgcagctc
     9001 tttttgtttt acatagaaac aagaaaaatg aaaaggcctt tttaggaagg cgcgagcgcc
     9061 ttctcaattg aaaaacctct ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt
     9121 gaacgttgca cgttcactaa gataaagggt cttgcaaagc aagacaaaat agaaaaaact
     9181 tcctctcacc aaaaggttcg ttcctgttcg ttatctcttt attgatttgc cagaccggca
     9241 acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc
     9301 ccttgtcctt gtcgcttttc gttgattcat tctggccaac aaatcaataa agaaagaact
     9361 aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg aaagaaattt
     9421 gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc acatactgtc
     9481 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca
     9541 acatgcggga aaaaatatac cttgtcttat ggcgattata ttttgattga tgagaaagga
     9601 aagaaacgtt gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt
     9661 tggtggacaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt ccgcggtact
     9721 ggacgacaga aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga
     9781 agattgcagg atatgaactg cattatcaag aaaacttgga aacttatgaa aacgaatatt
     9841 ggttaaccga attagagaac taaaaaggag aaaaaaatat ggctcaaaat tactatgacg
     9901 aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg acattcctat
     9961 acaatgagac acgtgttcca aaaaaacatt ataaggaaag attgtcggta gctgtggaag
    10021 aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtattatcga acactggaag
    10081 aactgaaaaa gcaaaatgca aaatggtttt tccaagcctt actatgcttg gaagtaggcg
    10141 tcaaaccaag cacaatcaaa ccttctgaat accaagcttt agaactaacg tatgcgaaat
    10201 ttatcgaaac gaaaaaagct aagactgttt caagtgaatg gttagattat tttgaaaata
    10261 tcaataagta tggcgcatgt tacatcatga agtaatcaga cggattcaaa aagactttag
    10321 ttgtatggaa gaaatgaagt actttgttca aaatgttgaa gaacagttat atttggaaag
    10381 cgaaacgagt caaacaaagg tcacagtcag ccataatcgt ttagatgtgt ataaacgtcg
    10441 tcctgaatca ttaaaaaaag gagcggagct tctacgacta gaaacagaaa aatacgtcat
    10501 gaatgtttac ctagaaaaaa gtgagttggt cgaaaatttt atggactagc cgagaacacc
    10561 cgtgatttta atcatgggat gaatcggatt cagttgtgac ctagctttag ctaggttaaa
    10621 caactgacaa atataaacta aataatagtg aaatgtcttg caataattga ttttgtagaa
    10681 atacatatat taaaataatt gcgaggtgtt tttgtatgtc agaaattcat tatggacggg
    10741 gttatgtcta ttctattcaa tatcatcttg tttggtgtgt aaaatatcga catgatgttt
    10801 tgtatggtca ggtagatatt gatgtcaaac agtttttgaa acaaattgct gatgataata
    10861 atataaaaat tatagaaata gaatcagata aagaccatat tcatttactc attgaatgca
    10921 ccccacaaca ctatattcct agtattgtta aagtatttaa aggcgcatcc gctagactgc
    10981 tgtttaaaaa acatcctgaa cttaaacaac tcctttgggg tggacatctt tggaatccaa
    11041 gctactttgt agctactgtt agcgaaaata cagaagaaca aattagaaaa tatatacaaa
    11101 atcagaaaaa gaaatgaggt gaatccgtat gctacaacat agggcttata aatttagaat
    11161 ctatcctaat caagaacaag aaatattaat gactaaaact attggctgtt caagatttgt
    11221 ctataattat tttttgaatt tgtggaatca tgaatatacg actactggaa aaggattatc
    11281 ttataattct tgttctgcca tgcttccaca aatgaaaaga aatgcagaga caatttggtt
    11341 aaaagaagtt gacagtattg cgattcaatc ctcattgaaa aatctttctg atgctttttc
    11401 tcgcttcttt aaaaagcaaa ataaacgacc acaatttaaa agtaaaaaac atcctataca
    11461 aagttataca actaaaaatg tgaataatag tatcgaaatt agaaacaatt tcgtaaaact
    11521 acctaagtta ggatttgtta aatttgcgaa aagtcaagag cccaaagggc gaatcttaaa
    11581 tgctacgatt cgaaaaaatt cgagtggtaa attttttgtt tccatcctgt gtgaagaaga
    11641 aatagttgag tttcctaaat ctaattccgc tattggaatc gaccttggta taactgattt
    11701 tgcaatcctt tctgatggtc aaaagattga taacaataaa ttcacgtcaa aaatggcaaa
    11761 aaaactcaaa cgtgaacaac gtaagttgtc aaaacgtgca ttactagcta aaaagaaagg
    11821 cgtcaatctc tttgaagcta aaaattatca aaaacaaaaa cgaaaagtag cacgactaca
    11881 tgaaaaagta atgaatcaac gcaatgactt tttaaacaag ttgagcacag agattatcaa
    11941 aaaccacgat attatctgta tagaagactt aaatacaaaa ggtatgttgc gtaatcataa
    12001 actagcaaaa agcatttctg atgtgtcttg gtctagtttt gtgactaagt tacaatacaa
    12061 agctaactgg tacggtcgga aaatcatcaa agtagataaa tggtttccat ctagtcaaat
    12121 ctgttcaaat tgtggacaca aagacggcaa gaaatctcta gaaattcgag aatggacttg
    12181 tcctgtttgt catgctcatc atgaccgaga tatcaatgct agtatcaata ttttgaccga
    12241 aggtctaaga ttacatgtca tgggattagc ttagatccaa tcatcaaaaa ccgtaggaac
    12301 tacggggata gcttggtaaa taagagaaac cgctgtgagt aaagaaatac gcttgcaagt
    12361 atgctctatt cccaagaatc tcgtgacttt agtcatgaga ggttcaagga ttcatggaat
    12421 gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt atgtatgtta
    12481 tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa aaaaatgggg
    12541 aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa ttcatacaga
    12601 agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa ttatcgaagc
    12661 ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca ctgatgaaag
    12721 aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa
    12781 cctagacaag gcgatattct attattgaat acagcacctc gatctggaca cgaacagaca
    12841 ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag taacgttgtt
    12901 acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa tataaactca
    12961 gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac aattgattat
    13021 gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga ggagttattg
    13081 atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag cagcatgatc
    13141 aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg tctcgtaaca
    13201 ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc aacgatgttt
    13261 ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt aggacgtcct
    13321 ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg tttagattcg
    13381 gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt aatgaattta
    13441 tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat gatcagctgc
    13501 acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
    13561 cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag tgcttcttga
    13621 aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat tctctctgca
    13681 ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga tgagacacgt
    13741 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa acaccctaat
    13801 catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg gtatacctta
    13861 tatagacaaa aactaatgat ggaggaaata gtatgagaat tttttgtaag tgtctactga
    13921 taacttattc tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact
    13981 ccaaaaaagt ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt
    14041 agattttcaa attaattagt aaagtgatag aataaattga tcatacaaat caaaagaaga
    14101 agcagggtga tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca
    14161 atatcgaaca tatgtttgta taatgtgaaa gaatggagtg ttatttatga tggattattc
    14221 aaaagagcca gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga
    14281 atgtatagaa agaaatttag atccattaac aacagaactt gttgttatga gtcgagctga
    14341 taatactggt tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat
    14401 tacaaatgtg agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt
    14461 tccaccacgt atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag
    14521 atatgtggct gatgaagatc tactgattta ctcgatcgat gaatcaatct taaaagtgac
    14581 ccgatcactg aatcttttta cgactgaagg aacacgaagt caacggagaa agaagctggc
    14641 tcaaatgatc caggagaata ttaaagaaga gctaggattg attgctacag taggtgtcgg
    14701 agataatccc cttttggcta aactagcctt agacaacgaa gcaaagcata atgaaggatt
    14761 tgttgcggaa tggacgtacg aaaacgttcc tgaaaaagta tggaatattc ctgaaatgac
    14821 ggacttttgg ggaattggct ctcgaatgaa aaaaagactc aatcagatgg ggattatgag
    14881 cattagagat cttgctaact ggaatcctta tacgattaag aatagactag gtgtgattgg
    14941 cttacagctg tacttccatg ctaatgggat cgatcgaaca gatatagcaa tcccaccaga
    15001 accgacaaag gaaaaatcat atggaaattc acaagtgtta ccacgagatt atactagaag
    15061 aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa gtagcgatac gcattaggca
    15121 gcataactgt aaaacaggtt gtgtccattt acacattggc acgtcgatct tagaaacgag
    15181 accagggttt tcacaccaga tgaagatccc tatcacagat aatacaaagg aattgcagaa
    15241 ttattgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc atgttggaat
    15301 aacctatagc aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca
    15361 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
    15421 ttttacgtcg attgtacatg cttcttcaat gttagaaggc gctagaagca ttactagaag
    15481 cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg
    15541 atcgtggacg tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg
    15601 ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga
    15661 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag
    15721 aacgaaacgc tgaaggatac tatccagaag atatcattgg caagatctta ggtagtgatg
    15781 agctggggat ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat
    15841 tttaccagga taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa
    15901 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa
    15961 tgaggttggt gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt
    16021 taggttttat atcttcccgt tatatatata aggtatttta cggattctat agagttatct
    16081 ctatgaatcc ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa
    16141 actattattg caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac
    16201 atttgtttac tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga
    16261 acaattttgt gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca
    16321 acgtagaata aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt
    16381 aaacatgtct gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa
    16441 gattaaacat gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat
    16501 tacgatatct taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa
    16561 gaagggaatt attagtaaca atatagacag tttagtaatg tttaaattat tctagttgct
    16621 gctcgcaaaa
//
LOCUS       DAFFQY010000059        16590 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFFQY010000059 DAFFQY010000000
VERSION     DAFFQY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.059"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(45..779)
                     /locus_tag="LRB02_001830"
     CDS             complement(45..779)
                     /locus_tag="LRB02_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL4660025.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1104..1514
                     /locus_tag="LRB02_001831"
     CDS             1104..1514
                     /locus_tag="LRB02_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL4660026.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1507..2208
                     /locus_tag="LRB02_001832"
     CDS             1507..2208
                     /locus_tag="LRB02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL4660027.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2208..3821
                     /locus_tag="LRB02_001833"
     CDS             2208..3821
                     /locus_tag="LRB02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660028.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3811..4032
                     /locus_tag="LRB02_001834"
     CDS             3811..4032
                     /locus_tag="LRB02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL4660029.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4029..4790
                     /locus_tag="LRB02_001835"
     CDS             4029..4790
                     /locus_tag="LRB02_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL4660030.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5171..5680
                     /locus_tag="LRB02_001836"
     CDS             5171..5680
                     /locus_tag="LRB02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL4660031.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5750..6289)
                     /locus_tag="LRB02_001837"
     CDS             complement(5750..6289)
                     /locus_tag="LRB02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL4660032.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6429..7040)
                     /gene="rpsD"
                     /locus_tag="LRB02_001838"
     CDS             complement(6429..7040)
                     /gene="rpsD"
                     /locus_tag="LRB02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL4660033.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7302..8153
                     /locus_tag="LRB02_001839"
     CDS             7302..8153
                     /locus_tag="LRB02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL4660034.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8181..8816
                     /locus_tag="LRB02_001840"
     CDS             8181..8816
                     /locus_tag="LRB02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL4660035.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8836..9609
                     /locus_tag="LRB02_001841"
     CDS             8836..9609
                     /locus_tag="LRB02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4660036.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10017..10814
                     /locus_tag="LRB02_001842"
     CDS             10017..10814
                     /locus_tag="LRB02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL4660037.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10792..11205
                     /locus_tag="LRB02_001843"
     CDS             10792..11205
                     /locus_tag="LRB02_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4660038.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11189..13834
                     /gene="mutS"
                     /locus_tag="LRB02_001844"
     CDS             11189..13834
                     /gene="mutS"
                     /locus_tag="LRB02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL4660039.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVPQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13852..15960
                     /gene="mutL"
                     /locus_tag="LRB02_001845"
     CDS             13852..15960
                     /gene="mutL"
                     /locus_tag="LRB02_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL4660040.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15982..16542
                     /gene="maf"
                     /locus_tag="LRB02_001846"
     CDS             15982..16542
                     /gene="maf"
                     /locus_tag="LRB02_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL4660041.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5385 a         2965 c         3599 g         4641 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaagaaaca tcaacacgaa aagcatcggt
      841 gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa aaaaatgaat
      901 tccattactt ataagaaacc agaccaacag taaaactcca actcttttta aaagttctcc
      961 acaaaccacc aagcttaact ataaatatac caaaaaaaaa acgttttttt tctcaaaaat
     1021 tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt aaaatgaaag
     1081 ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt attattttct
     1141 tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct ggaagtatct
     1201 taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct gaaaaaatcg
     1261 gagcaactgg tgatttttta ttgaataatc tagctatttt atttgttcct gctggtgtag
     1321 gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg ggagcagtcg
     1381 tcgtgtgttc catcgtcacg atggttgcag agggaaaaac ggcagaaggt gtagaagctt
     1441 tgctgggcta tgtccgtaag aaaaaagcag gaagggtatc tgtaaacgaa atggaggaaa
     1501 atcaaattga ctgagttttt tgtgaatcca ctatttggct taagtttgac tttaggagtg
     1561 tatgtattat ctgataaatt tttgcggaaa tataaactgc ctgttttgaa tcctctcgta
     1621 ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta taaagactat
     1681 aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc acttgccatc
     1741 cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat ggctgcgatt
     1801 ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt cttctctctt
     1861 cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat ttctgtcgat
     1921 ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt ctctacaggt
     1981 atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga aagtccagtt
     2041 gctcgcggtg tggcgttagg aagtacttct catgctatcg gtacagcaaa agcaatcgag
     2101 attggcgaaa tagaaggaat catttccgga cttgcgatat gcgtaaatgg gatattgacc
     2161 gttttgttgc tgccgttatt ctttcagcct tttgctggct tgttttgatg cgaaaaccac
     2221 tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa gacgtgtatt
     2281 tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt ctcctcctag
     2341 aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt gttcagctgg
     2401 atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag cagtttagcg
     2461 gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt tatgatacgc
     2521 tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg ctgaaattcc
     2581 agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt gcgcaaaagc
     2641 attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag caactgcaaa
     2701 cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa gaaatcattc
     2761 gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg ccatttgaaa
     2821 cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt tttaatgttc
     2881 gtctgaacga gatcaaaaaa agacgattgg aatacatgct aggtgccttt tttttaagaa
     2941 aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt tctgacaatc
     3001 tgttgttcca gcgattttgc caagtcatgg agccggtatt tcctaattat tttcaagtag
     3061 aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat tatagtgatc
     3121 ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg ccctttacag
     3181 ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg ctgccagcag
     3241 aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc gcctatcttt
     3301 ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat catttagtta
     3361 ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga cacaaacatc
     3421 ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg tcggtatctg actgtgttta
     3481 aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta atgacagatt
     3541 tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc cgtaatgagt
     3601 atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt ctgatttcga
     3661 caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg accgaagaac
     3721 taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa aaaaaaacta
     3781 ttaaaacaat aacaaagaag gagaaaaaag atgacatttg aagaaatttt accagaattg
     3841 aaaaaaggtg caaagattat tcgtaaagga tggagtggat ttgaacttta tgttgttctc
     3901 gtatccgatg atgaatttga tggctgtcca gtgacacctt acttattgat caaaacaagt
     3961 gacgaaggtt tttccagctt cgctcctaca gtttgtgata ttttagcaga tgattgggcg
     4021 atcgtagaat gaaaaaccag tatccagatc tagctaaaca ggttgtattt atcacaggag
     4081 ctgcttctgg aattggtgct gcgcaagccc gagcgtttct tgaacaagat gcttttgtat
     4141 ttggattaga tcaaaagttc ggtaaaatgg aagaactctc tttgcgattt cctgaaactt
     4201 ttgcctacgc actaggtgat gttcgacaaa tgaaagaact ccaacaagcg gtatcgaaat
     4261 gccaggatcg ttttggtgaa gttacgattt tactgaatac atcggggata ttggatgcct
     4321 acaaaccatt gatggaaact gacgaatcgc tgtgggatct tatttatgag acgaatgtga
     4381 aaagcatgta tcagctgaca aaaatcatcc ttcctgatat gatccgccga aaaagcggaa
     4441 caatcatcaa catggcttcg attgcgggtt tggttgctgg cggaggtggg atcgcttata
     4501 caagtgcgaa acatgcaatc gtcggattta ccaaacaact cgctctggat tacgcaggac
     4561 agggtatccg agtaaaagga attgctccag gagccatcca aacaccaatg aatgcggctg
     4621 attttgaagg gaaaggtgag atggctgagt gggtcgcaaa tgaaacaccg gtcaagcgat
     4681 gggcacagcc ggaagaagta gctgagctga cgttatttct tgcctctcct caagcaagct
     4741 acatacaagg aacgatcatc ccaatcgatg gcggatggtt gttaaaataa ccttgtgttt
     4801 ttttcaagct tctgtatgat aaaactaaag agagaaacaa cggttgatga taaaagaaag
     4861 tcgatgactg ttcccgacat tcgatgccga acagccagtt caaaaaattc tttttaataa
     4921 tcgttaatat tcgaaactaa gcggcttttc acgacctttt gatataaacg gtgttcaaaa
     4981 gcttgcaact gcggtaacaa gtccagccaa atgaaaaata taaggagcta tttttatttg
     5041 actattctta ttacttgttg cagggcagct ttttcactac cttctgcggc acttcagttg
     5101 gaattttctt cccatccttt ggtcgcagtc attaccaaga taaaattatc ttgcaaagga
     5161 gaaatagaac atggaaaaaa ataatacccg ttcagccaaa cgctggacaa cgacagatgt
     5221 gactcggatg gcgctagtca ctggtttata tgtggcagtg acgctggttt tgtctgtctt
     5281 tagctttgga gtgattcaga ttcgtctctc ggagatgttc aactatctgt ccttgtataa
     5341 caaaagatac atcgtcgcgg taacagcggg agtagctttg gctaatatgg cttctcctct
     5401 tggactgata gatgttgtag taggaagcgt cagcacgctg cttgtattgc ttatcaacta
     5461 taaaatcact agtcgcatca aaaacatgaa aatcaaaatg gctgtgacag catgcgtatt
     5521 tgctttttcc atgtttacag tagctggtca gctaacgatt ttatatcaac tacctttttt
     5581 cttgaattgg tgggtgattg ctttaggaga attggcatca atgattgtcg gtgggataat
     5641 tatttattgg atcggtaaaa aagtggattt gtcaaaataa agatctgttt aatttaaaat
     5701 atggcagcgt ggaagagact ttgagtcttc ttccatgctg cttttttgat taatttgtat
     5761 gaagaaatcg atttagccgt tttttcatca agataccgat ataagctgct ccagttgcct
     5821 tgattgcttc cggcagtaaa aacggcagta gtccactggc aaatgcagtt gtaaaagtaa
     5881 gatcaccact tattttcagc cagatcgttc cgcagatcaa tgcagcaaga gagcccatta
     5941 gattggcaaa gattgcatat aagtaatgag catccgttct ttcgatcatc caaccagtta
     6001 tccacgtttg taagagaaat ccaatcaaat atccgccagt tggcccaaat aaaacagcta
     6061 tacctccagt catccctgaa aatacaggaa gaccaatcaa gccaagtaaa agataaacta
     6121 caacagatat caatcctagt tttcttccga gaaaagttgc tgttaatcca atagcaaatg
     6181 tttgccccgt caaaggaacc aagccaaaag gaatggtcat ctgagaaagg acagaaataa
     6241 tcgcggcaaa ttccgcagca agtattaatt ctttcaatgt tcggttcata atgccctctt
     6301 ttcgttaact taatattttt atttggttaa cgaaattgta aaacaaagtg attactttgt
     6361 aaatagaaaa tataaaaaaa gaccagctat ccaatagata gcggtctttt tcaagaaagc
     6421 ttttgaaatt ataagctttg gttgtagtat tcaacgacaa gagcttcgtc gatttctgga
     6481 tatagttcgt cacgttctgg taaacgagtc aatgaacctt ccaatttttc agcgtcaaag
     6541 cttacgaatg ctgggcgtcc aacagttgct tctacagctt cttttactgt tacgatgttt
     6601 tgtgattttt cacgaactga gatcacttga cccacttcta cgtggtatga agggatatca
     6661 acgcgtttgc catcaactaa aatgtgacca tggttaacta attgacgtgc ttgacgacga
     6721 gtcgttgcaa gtcctaaacg gtaaacaaca ttgtctaaac gttgttctag taagatcatg
     6781 aagttaacac catgtttacc ttctttgatt ttgctagctt ttacgaacaa gttacggaat
     6841 tgacgttcgt tcattccgta catgtgacga agtttttgtt tttcagttaa ttgcatgccg
     6901 tattctgatt gtttgccacg gctgttaggt ccatgttgac ctggtgcgta agggcgacgt
     6961 gctaattctt ttcctgtgcc tgacaatgag atgcctaagc gacgagaaat tttccatgat
     7021 ggtcctgtat aacgagacat taaaatttcc tccaatgaat ataagttttt ggagtaaaat
     7081 aatccgttga aaaattcata atcatatagt ttgttcttca atcttcacct ttgcagccgc
     7141 ggttacacaa tcgaacccga gacggggcaa caaactaggg atgagttatt atttttctgc
     7201 tgcattattt tacacaaact acagtatatg atagatcatc cacgaatgtc aagataatta
     7261 gtacataaaa acgctttaca aacctaggct gtctttataa aatgagaggg catgtgcaaa
     7321 tggaaaaaag cgaaggaagt ttaaggagaa aatcaatgaa tccggtatca ccattatttg
     7381 agaaaatcga tgcttcaatt gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt
     7441 acgctgttcg agcggttctc gcctgtttgt ttttgacttt aggaacggct gtggcatttg
     7501 caatcgcgat gaagggggaa gatatcgcgc atggtctagg aaagatttta tacgcattta
     7561 tgttcagctg gggtctggtc atgattctat acatgaacgc tgaattagga acatcgaata
     7621 tgctttatat gaccgtgggt gtttttcggc agagacttgc tttaagtaag gcagccaaga
     7681 ttctatttgc ctgtattttt ttcaatatga ttggcggtat attatgtgga tttcttattt
     7741 ctatgactac gccatttcaa gatttaacgt tggacaactt tatgttagaa tcaatagcag
     7801 taaaactagc aaaacctgca gcaacgattt tggtggaagg gatgtttgcg aatatcgttg
     7861 tcaataccgc tgtactgatc agcatgcgga tgaaagagga tgccgggaaa gtgatcacag
     7921 tcgtattcat cattttcatt tttgcttttt taggatatga acacgtgata gcgaattttc
     7981 ctgcctttac gcttgcttac tttgcttctc aaggacagac ggatggaatg acagtcagca
     8041 atgtgttaca taatttgttt tttgctttgg caggaaatta tattggtggc ggtttagtaa
     8101 tgggactggg atatgcttgg ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg
     8161 ttcattaaag gggaagtgcc atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt
     8221 tacaaatgga aaagtacatg aaaaatcttt tttcctttgc tggagtacct aagccggaac
     8281 gcgctctttt acaacaaccg ttactggatg agagcaaaaa tttatctatt tctgaagttt
     8341 tagcttttgt ttattattat tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg
     8401 atttagtcca gaaaaacatc aaacggctta catttgaaga cttagtcgta ttgttgccac
     8461 tagttcagaa aaaagaatgg tgggacagca tcgactcttg gagaaaagta tacagcacat
     8521 ggtgtaaagc ctacccagag cagcgtgaag aagtctttca attgtttgaa aaaaaggaag
     8581 atttttggct acgtcgaata gcaatcacgt tgcaattagg ttataaacag caaacagaca
     8641 caggactatt gatccgagca atagacgaag atcgtcaaac aaaggaattc tttatccaaa
     8701 aagcaattgg ttgggcatta cgcgattaca gtaagaccga tgccgattgg gtaaaaagac
     8761 agctgacaac agatttgagt aagttagctg ttcgagaggc aagtaagtat ttataagaaa
     8821 gcgagacgac aagagatgag acgcaaatta tttgcttttg atattgatgg aacgttgtta
     8881 gattcaaaca aacaagcttt agatagcacg agagaagcat tggaaaaatt acgcaaagcc
     8941 ggtcatctgg tcacgattgc tacagggcgg agccgatttc atgcgcaaga gatcattcgc
     9001 gatcttgatt ttacgaacta cattttatgt aatggagcgg ccggtttttt agaccatgaa
     9061 caagtatata agaatttact agatcgcgac cagttggatg cgttcgttca agaagcgcat
     9121 catgagcaga tcgatacagc ttttgtcagc ttagacaata tcaagcgttc ttcttccaat
     9181 gatgtaacgg tgatggaaga agcgatgcat tctttcggtg ctgtcttgcc tgaattagat
     9241 cagttttttg cagaggaaca agaaatatac caagcgttag catttttcga tcaaacatat
     9301 gatgggaagt tctcttcata tgataaaatt cgatttgtcc gctggcatga gaacagtgta
     9361 gatgtcgtac ctaaaaatgg ttctaaagca gcgacaattc tgcacttagc agaacaagta
     9421 ggcatccctc atgaagacat catcagcttt ggcgatggac aaaacgaccg tgaaatgttg
     9481 cgcatgtcag gaattgggat agctatgggg aatgctgtgc cagaagtaca agcagaagct
     9541 aagatggtca ctgatacaaa cgatcaggat ggaatttgga aggcattgaa agagttaagc
     9601 gtgatttaaa agaggttgtg aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca
     9661 aaaatagctc ctcctatttt tcgtttttct gggcttatta ccgcagatgc aagcttttga
     9721 acaccgttta aggaagaggt cgtgacagaa gtggtcagtt tcaagaaata agaagggatt
     9781 tacgaaaatt gcttctcaaa tttttgtaaa attacggctt atttctgaag aagtagatga
     9841 aacaacgttt attcgggaaa tgggactgtg acaagatgtt tgtcatagtc ccattttttt
     9901 attttatttt tcaaaactaa gaaataccag gaaagagttc cttttgtttg accttttctt
     9961 tcttattgaa aacaactata atatagagtg atgttaaaag agaagaggaa tttagtgtgc
    10021 gggtattgtt tattggtgat gtagtaggtt caatgggacg agagatgatt actgaatatc
    10081 ttcctagatt gaagaagaag taccgtccgc aagtaacgat tgtaaatggt gagaatgccg
    10141 cttctggacg tggaatcaca gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg
    10201 tcgtgacgat ggggaatcat acgtgggaca accgcggaat ctttgagttt gtaaatgaag
    10261 ctaaaaagat ggttcgtccg gcaaattttc cggaagggac gcctggacaa gggatggtct
    10321 ttgtcaaagt gaaccaagtc gaactggcag tgatcaacat gcaggctcgt tcttttatgg
    10381 tggacttgga tgatcctttt cgtaaaatga atgagctggt agaagaagcc agaaaacgta
    10441 cgccgatcat ttttgtagat tttcatggag aaacaacaag tgaaaaacaa gcaatgggct
    10501 ggtttttaga tgggaaagtt tcagcagtgg tcggtacaca tacacacgtt cagacaaatg
    10561 atgcgcgaat tttgccaaga ggaacagcgt atctgacaga cgttgggatg acaggtccct
    10621 atgatggtat tttgggtatg cgcagagaac ctgtaatcga aaagtttctg accgcattac
    10681 caaaacgttt tgaagttgtg gaacaaggcc gatcgatttt aagcggctgt atcttggaat
    10741 tggatgatac gacaggtcat gcaaaaaaaa tccagctgat tcagatcaac gatgatcgac
    10801 cgttcatgga gtagaaagag aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa
    10861 gtaaggaaac tgacaaaact tttaagagag aatgaaacga tcattcgcta caaagaactt
    10921 gaagaaaaga tccagcaaaa ccagtattta gctgagttga gagaaaaaat caaacaagcg
    10981 caaaaagatg ctgttcattt tgcccactat gacaaaccag cagcagaaca agaagcaatc
    11041 aaacaagcgg atcaattcat gcaagaattt gatcagcatc cgcttgttgt agcttataga
    11101 aaacagctgt tggaagcaga tgatctgctt catcatttaa caactatgat acaagaagaa
    11161 atcaatggac aaatagagga ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat
    11221 ggaacaatat ctaagcatca aggcccaata ccaagatgct tttttgttct atcgtttagg
    11281 agacttttat gaactatttt acgaagatgc gatcaaagcg tcacagcttt tggaattaac
    11341 gttgacgagc agaaatcgaa atgcagaaga acctattcct atgtgcggtg tacctcacca
    11401 tgcagcacaa ggatatatcg atgccttgat tgaaaaaggc tataaagtag cgatttgcga
    11461 gcaggtagaa gatccaaaga cgactaaagg gatggtcaag cgcgaagtga tccagctagt
    11521 cactcctggt acagtcatgg acagcaaagg attgtcggca aaagacaaca attttttaac
    11581 agctgtagtt agtcaaggaa atgaatttgg ctttgcttat gccgatttaa gtacgggaga
    11641 attgaagacg gctcggttgc atgatgaaga agctgtttta aatgaagcct ctgcattgca
    11701 gacaaaagaa ctcgtattag gcagtgagat cactgatacc cttcgtgaac agctaagcgg
    11761 gcgtttaggg ctagtctttt ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt
    11821 tttgacaagc gaactagtcg atccgctgaa aaaagaagca accggcaaac tattgactta
    11881 tttatcggtg acacaaaagc ggagtttagc tcatatccaa aaagcagtag aataccaacc
    11941 agatcatttt ttgaaaatgg attattattc aaaattcaac ctagaattaa gccaatctat
    12001 tcggactggg aaaaagcacg gcactcttct atggttgtta gatgaaacaa aaacagcaat
    12061 gggtggccgt ttactaaaac aatggctaga ccgtccgctt atccaggcaa aacaaattaa
    12121 agcaagacaa gaaatggtcg cttctttgtt agacagctat tttgaacgta tcgatttgca
    12181 gtcagcatta acgaaagtct atgatctgga acgcctagca ggtcgagtag ctttcggtaa
    12241 tgtcaatggg cgagacttga tccagctgaa aaattcttta gaacaagtgc caatgatcag
    12301 agggttgatt gaaggaatcg atcaaggtca atggcaaagt ctgctttcag aaatgcagcc
    12361 gatggaccat ctcgtccaat tgatcgacca agctatccaa gatgatccgc cgttacaaat
    12421 aacggaagga aacatcatca aagatggatt caatgagcag ctcgatatgt accgttctgc
    12481 tatgagaaac ggaaagaaat ggttggcaga acttgaagca aaagagcgtc aagaaacagg
    12541 aatcaaaaac ttaaaaatcg gatttaaccg cgttttcggt tattatatag aaatcacaaa
    12601 agcaaatctt ggaaatgcag aactggagaa gtatgaacga aaacaaactt tagcaaatgc
    12661 agagcgtttc atcacaccag aactaaaaga attggagaca caaatcctcg aagcagaaga
    12721 aaaatctgtt gatttagaat atcagttgtt tttagctgtg agagaagaag tcaaaaaggc
    12781 tatccagccg ctacaagtac ttgcaaaagc aatcagtgca gtagatgtgc cacaaagttt
    12841 tgcaacgatc agcgaacgct atcagtatgt tcggccagaa cttgtcagtg acaagcatca
    12901 gcttctgatc aaagacggac gacatcctgt agtagaaaaa gtattaggac atcaggaata
    12961 tatccctaac agcgtagaaa tggcagaaga cgaaatgatc ttgctgatta caggaccgaa
    13021 tatgtctggt aaaagtacgt atatgcgaca gttggcatta actgttctga tggcgcaaat
    13081 gggctgtttc gttccggcaa aacaagcagt tttacctatt tttgatcgga tttttacccg
    13141 aattggtgca agtgacgact taattgctgg acaaagtact tttatggtag agatgatgga
    13201 agccaatcaa gccctgcgtt atgctacgcc aaacagtttg attttgtttg acgaactagg
    13261 gcgaggaaca gcaacctatg acggaatggc gttggcccaa gcaatcatcg aatatatcca
    13321 tcgtcatgta caagcaaaaa cactcttttc tactcattat catgaactga cggtacttga
    13381 gaaagaactt ccgcagctta aaaatgtcca tgttggagcc gttgaaaaag acggagaagt
    13441 tgtcttcttg cataaaatga tggatggacc tgcggataaa agctacggca tacatgtggc
    13501 aaagatcgca ggattgcctt ccaatctact agaacgagca gcagatatcc tgcatacgct
    13561 agaaaatggc gataacggac accatactgt tccagctgaa gtagaagaga aaactaaaac
    13621 agccaaacag gagcaaataa aaccacctgc agaaaaagta aatcaagacc atatacaaga
    13681 ccgtgtaaaa gaagaaaacc aacagctttc tttattcagt gaattatccg aaaatgaaac
    13741 agaagtcatt gaatcactga agcaaataaa tttactagaa atgacgccaa tggatgcact
    13801 aaacacatta tatgaattac aaaaacaact gtaggaagaa ggcggatatc gatggccaaa
    13861 atccaagaat tatctgaacg tttagctaat cagatcgctg cgggagaagt agtggagcga
    13921 ccagcttccg ttgtgaaaga attagtggaa aatgcaattg acgccggcag tactcagatt
    13981 gatatcttag ttgaagaagc gggactgcgg acgatccaag tgattgataa cggagaaggc
    14041 attttggcag acgacgtaga gaatgccttc aagcggcatg cgacaagtaa gatccataat
    14101 cgcgacgatc tttttcgcat acgaacgctg ggctttcgag gggaagcttt gccaagtatt
    14161 gcctctgttt ctgaaatgac tctggaaacg gcgaatcagg aagaaaaaca aggcacctat
    14221 ctgtcgctga aaggcggaga agttattgag catcgaccag caccactgcg tcaaggaacg
    14281 aagattaccg tcagcaatct gtttttcaat acgccagccc gcttgaaata cgtgaaaaca
    14341 ctgcacacag aattagcgaa catcggggac atcgtcaatc gactggcatt gagtcatcca
    14401 tcgattgctt ttcgtttagt acatgatgga aacaaaatgc tggcaactgc tggaaatggc
    14461 gatctgaaac aaacaattgc gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa
    14521 atcgaaggaa aagatctaga ttttgagtta tttggctatg tttcattgcc tgaggtgact
    14581 cgtgccagta ggaattactt atcgacgatc atcaatggac gttttatcaa gaacttttcc
    14641 ttgaataaag caatcgttgc aggttatggg tcaaaattaa tggtcggtcg tttccctatt
    14701 gctgtattgg aaatcaaaat ggacccgctt ttagtagatg taaatgttca tccgacgaag
    14761 caggaagtcc ggctgtcgaa ggaaaaagaa ctaacagagc taattagtaa cgccattcaa
    14821 aaagcattgt ctcaagaaaa tctgattcca agtgcagcgg ataatctacg gtttaagaaa
    14881 aaagtacctg atcagcctaa aacagaacaa atcgaaatcg atttgtccta cgcttttgaa
    14941 gaagaaaaac ctaaaaagcc aagtagtttg aattttgatc gagagacagg tacctttttc
    15001 gtagaagaac caaagaacac atttgttgac agaattgttg ataagtctgt ggaaaatgtt
    15061 gaaaactatt ctggtttttc tgaagaaaac accacttatc cagaaaaaac agcgaaaacg
    15121 gtggataagt ttggtgaaaa tgttgataac tttgtagaaa aaacagtgga aaaatctgaa
    15181 aagaaagaat ttcccgttgt ggataacatg gagaaaacaa atgaagaaca agcagataaa
    15241 tccgtagaag aggatgaaaa gatttacgag gaagaattga cactccatcc tgaatttgat
    15301 ttcactcaag gaaacgacaa aacattagaa caagccatca agaaattaga aacggaaaaa
    15361 ccagtacaac gttttcctca gttggaatat tttggacaga tgcacggaac gtatcttttt
    15421 gcccaaagta gcgatgggtt gtacatcatt gatcagcatg ctgcccaaga gcgcattaag
    15481 tatgaatatt tccgggaaaa aattggggaa gtatctgatg atctgcaaga attgcttgtt
    15541 ccatttgtgt tagactatcc aaacagtgat gctttgaaat taaaagagca aaaacacttg
    15601 ctagaagatg cagggattta tttagaagaa tttggtcaaa acagctttat tgtacgggcc
    15661 catcctactt ggtatcccaa tggagaagaa gaatcgatta ttcgggagat gatcgatatg
    15721 ctgctgacaa ctggaagtgt gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg
    15781 agttgcaaac gatcgattaa agccaaccat tatttgaatg aagcacaagc acgtgtgctg
    15841 ctgaaggatc tggcacgatg cgaaaatcct ttcaattgtc cccatggccg tccagtattg
    15901 attcacttta ccaattcgga tatggaaagg atgttcaaac gaatccaaga cccgcattaa
    15961 gtcaaagaga agagggaaaa catgaagatt gtattagcat cccaatcacc aagaagaaaa
    16021 gagttattgg ctcatatcgt ttcagaattc gagattgaac ctgctgatat cgatgaaacg
    16081 ccatttccag acgaacagcc cgtcgattat gttcgtagaa tggctgaaga aaaagcacga
    16141 attgtctggg aacaagatga aaaagaagac catttagtga ttgccagtga cacgactgtt
    16201 gttctcaatc aagagatcat gggtaaacca gaagatctgg ctgaggctga attcatgctg
    16261 aaaaaactaa gcggggaaac acatactgta tatacagctg ttgttttaaa aacaaaggaa
    16321 cgagaagaac ggatactagc agaagcacat gtcacttttt atcctttgac agacgaagag
    16381 atcaaacgct acttggagac gggggattat gcggataagg caggggcata cggcatccaa
    16441 aatgctgcga gtgtattcgt caaagaaatt tctggagatt attacagtat cgtaggattt
    16501 ccgattggtg ctgtcaatca ggcactgaaa acattcattt aaacaaaaaa gcggactatc
    16561 tgattttcga ttgaaaatcc atgatagccc
//
LOCUS       DAFFQY010000060        16431 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFFQY010000060 DAFFQY010000000
VERSION     DAFFQY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.060"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(46..321)
                     /locus_tag="LRB02_001847"
     CDS             complement(46..321)
                     /locus_tag="LRB02_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL4660042.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(564..1874)
                     /gene="der"
                     /locus_tag="LRB02_001848"
     CDS             complement(564..1874)
                     /gene="der"
                     /locus_tag="LRB02_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL4660043.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2056..3285)
                     /gene="rpsA"
                     /locus_tag="LRB02_001849"
     CDS             complement(2056..3285)
                     /gene="rpsA"
                     /locus_tag="LRB02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL4660044.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3419..4099)
                     /locus_tag="LRB02_001850"
     CDS             complement(3419..4099)
                     /locus_tag="LRB02_001850"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL4660045.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4148..4783)
                     /locus_tag="LRB02_001851"
     CDS             complement(4148..4783)
                     /locus_tag="LRB02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4660046.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4849..6252)
                     /locus_tag="LRB02_001852"
     CDS             complement(4849..6252)
                     /locus_tag="LRB02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL4660047.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6239..7270)
                     /locus_tag="LRB02_001853"
     CDS             complement(6239..7270)
                     /locus_tag="LRB02_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660048.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7301..7522
                     /locus_tag="LRB02_001854"
     CDS             7301..7522
                     /locus_tag="LRB02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL4660049.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7687..8424)
                     /locus_tag="LRB02_001855"
     CDS             complement(7687..8424)
                     /locus_tag="LRB02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL4660050.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8577..9917)
                     /locus_tag="LRB02_001856"
     CDS             complement(8577..9917)
                     /locus_tag="LRB02_001856"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL4660051.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10154..11677)
                     /locus_tag="LRB02_001857"
     CDS             complement(10154..11677)
                     /locus_tag="LRB02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL4660052.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12055..12666)
                     /locus_tag="LRB02_001858"
     CDS             complement(12055..12666)
                     /locus_tag="LRB02_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4660053.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12789..12914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12996..13712)
                     /locus_tag="LRB02_001859"
     CDS             complement(12996..13712)
                     /locus_tag="LRB02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL4660054.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13718..14287)
                     /gene="scpB"
                     /locus_tag="LRB02_001860"
     CDS             complement(13718..14287)
                     /gene="scpB"
                     /locus_tag="LRB02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL4660055.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14271..15068)
                     /locus_tag="LRB02_001861"
     CDS             complement(14271..15068)
                     /locus_tag="LRB02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL4660056.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15038..15409)
                     /locus_tag="LRB02_001862"
     CDS             complement(15038..15409)
                     /locus_tag="LRB02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL4660057.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15428..16315)
                     /gene="xerD"
                     /locus_tag="LRB02_001863"
     CDS             complement(15428..16315)
                     /gene="xerD"
                     /locus_tag="LRB02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL4660058.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4612 a         3564 c         2785 g         5470 t
ORIGIN      
        1 aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc
       61 ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc
      121 accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa
      181 accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc
      241 atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat
      301 taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat
      361 atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt
      421 caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt
      481 cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa
      541 aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc
      601 ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc
      661 atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta
      721 gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca
      781 tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact
      841 tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg
      901 attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt
      961 gtattggttt ctttctttac aagatcccat ttattcacga cgataataat tccacgacca
     1021 gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc
     1081 agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat
     1141 ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag
     1201 aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca
     1261 atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca
     1321 acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct
     1381 tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct
     1441 gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt
     1501 tctggattat ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt
     1561 ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt
     1621 acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca
     1681 tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct
     1741 tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca
     1801 ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt
     1861 gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat
     1921 gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga
     1981 aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca
     2041 cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt
     2101 caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata
     2161 agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact
     2221 caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc
     2281 ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac
     2341 tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac
     2401 tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg
     2461 ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt
     2521 gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga
     2581 aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc
     2641 agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc
     2701 ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt
     2761 ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa
     2821 gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc
     2881 aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg
     2941 gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag
     3001 gtagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat ctaatacatc
     3061 accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg
     3121 aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac
     3181 taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc
     3241 ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct
     3301 aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa
     3361 taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt ttcttaattt
     3421 aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa
     3481 tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt
     3541 cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag
     3601 tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg
     3661 ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt
     3721 ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga
     3781 cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta
     3841 aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga
     3901 taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg
     3961 taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa atataattca
     4021 attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg
     4081 caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg
     4141 gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta
     4201 agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg
     4261 gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg
     4321 tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg
     4381 gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag
     4441 attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg
     4501 tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat
     4561 tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga
     4621 ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag
     4681 aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg
     4741 tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac
     4801 tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat
     4861 gtttttttta aataaattta gtaaaatcgt ttcccaatga agttgttcag tcttagaaaa
     4921 aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg
     4981 cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc
     5041 attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag
     5101 ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg
     5161 cagctcatca aagggagctt gctttgtggc atattccaaa taaagctcaa aactttgtcg
     5221 ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa
     5281 aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt
     5341 ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc
     5401 cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg
     5461 aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc
     5521 cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc
     5581 tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac
     5641 aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa
     5701 tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt
     5761 gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc
     5821 aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt
     5881 tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt
     5941 atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa
     6001 agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga
     6061 aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca
     6121 aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc
     6181 ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc
     6241 atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt
     6301 ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat
     6361 ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat
     6421 catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac
     6481 ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca
     6541 tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg
     6601 gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaacct
     6661 ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag
     6721 ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca
     6781 aactctcata cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt
     6841 tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg
     6901 ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg
     6961 ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat
     7021 cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg
     7081 caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga
     7141 taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa
     7201 ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa
     7261 aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact
     7321 tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga
     7381 ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt
     7441 gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat
     7501 tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct
     7561 aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga
     7621 aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca
     7681 tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg
     7741 gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc
     7801 tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc
     7861 ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg
     7921 aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc
     7981 ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa
     8041 tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt
     8101 gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt
     8161 ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag caccaggata
     8221 aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc
     8281 ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt
     8341 ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat
     8401 tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt
     8461 cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc
     8521 aaataaaaaa agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat
     8581 ttagcacata aacgatacac agcttctgca taaatttcca ttgctttata catactactt
     8641 aattcccatc gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg
     8701 aatgccacac actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca
     8761 gtcatgtctc cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct
     8821 agtggcacgt ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct
     8881 gctgttttag ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata
     8941 tctacaccga tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc
     9001 tcgccggatt gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga
     9061 actaattttc ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc
     9121 agacgagaaa tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga
     9181 acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt
     9241 acttcagaaa gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct
     9301 ccagctttta cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct
     9361 ttttctgcat aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct
     9421 tctttttcgt tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata
     9481 aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt
     9541 gccgcaatag aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt
     9601 atagttggat cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc
     9661 atatgacaca agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag
     9721 ccttctggat ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc
     9781 aaagcttcaa tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt
     9841 acggaaggaa ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct
     9901 gtcacaaatt ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc
     9961 ctccgacaca acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca
    10021 gaatgttctt aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttgtc
    10081 cacgtgctta tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg
    10141 tatttgtcta ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata
    10201 atatcgcaat taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg
    10261 ctacagcacc cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag
    10321 attgataagc agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca
    10381 tgattcccat tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga
    10441 tatagaaaat aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt
    10501 cgccccaatg taagatggtg tctgtgatta atccattgtt cataaccact tggataccac
    10561 gagcaacagc tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag
    10621 agacaaattc agactctttc atgccataag tgattgctac tacgatggac attactaaga
    10681 ataacatagt aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa
    10741 ccgtacctag taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat
    10801 tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga
    10861 acaatacatt aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa
    10921 tatcgaaatg cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt
    10981 tttcgatctt tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga
    11041 acatcaagaa tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta
    11101 cgcctgttgc aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata
    11161 cgattgctac agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag
    11221 catagaatgg aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca
    11281 gcatcaatac tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa
    11341 tcccggcatc tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa
    11401 tgaagaaaga gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac
    11461 ctagcatccc attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta
    11521 ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg
    11581 gtataaacca agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg
    11641 aaggcatttg aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt
    11701 tagagacaac aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata
    11761 ataacacgca ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct
    11821 tttttcaggt ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac
    11881 aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt
    11941 tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt
    12001 cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt
    12061 gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg
    12121 cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac
    12181 gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc
    12241 taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt
    12301 tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt
    12361 tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt
    12421 gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa
    12481 gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat
    12541 atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg
    12601 taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt
    12661 cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc
    12721 aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt
    12781 caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc
    12841 agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc
    12901 ccctgaagac gaaccttata taatttttgt ttacatattc attatacata tctattcaaa
    12961 aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg
    13021 ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga
    13081 tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt
    13141 ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta
    13201 ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt
    13261 ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa
    13321 actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc
    13381 agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt
    13441 accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata
    13501 actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca
    13561 tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg
    13621 tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca
    13681 gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta ttcattccat
    13741 caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag
    13801 gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata
    13861 atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta
    13921 gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga
    13981 ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg
    14041 atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat
    14101 aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt
    14161 ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt
    14221 cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt
    14281 tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt
    14341 tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca
    14401 acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt
    14461 tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc
    14521 gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc
    14581 aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg
    14641 atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt
    14701 tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga
    14761 tcttcttccc ataattcttc atcgtctgtg ccgaattctt ggacaggaag caatgtcttg
    14821 cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg
    14881 agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata
    14941 tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg
    15001 aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg
    15061 ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt
    15121 acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat
    15181 tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc
    15241 cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt
    15301 cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca
    15361 atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt
    15421 cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga
    15481 tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc
    15541 gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg
    15601 ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt
    15661 gccgagaaag acctttccca tgattattga caaatacatg catttcagat gcgtccttta
    15721 caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta
    15781 aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat
    15841 gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga
    15901 cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca
    15961 gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat
    16021 cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc
    16081 ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact
    16141 tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct
    16201 cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg
    16261 acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa
    16321 tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc tttttccata
    16381 atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt t
//
LOCUS       DAFFQY010000061        16271 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFFQY010000061 DAFFQY010000000
VERSION     DAFFQY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.061"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(56..751)
                     /locus_tag="LRB02_001864"
     CDS             complement(56..751)
                     /locus_tag="LRB02_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4660059.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            905..1183
                     /locus_tag="LRB02_001865"
     CDS             905..1183
                     /locus_tag="LRB02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660060.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="LRB02_001866"
     CDS             1202..2410
                     /locus_tag="LRB02_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4660061.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="LRB02_001867"
     CDS             complement(2558..2923)
                     /locus_tag="LRB02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL4660062.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="LRB02_001868"
     CDS             complement(3070..3843)
                     /locus_tag="LRB02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4660063.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="LRB02_001869"
     CDS             complement(4017..4877)
                     /locus_tag="LRB02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL4660064.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="LRB02_001870"
     CDS             complement(4877..6295)
                     /locus_tag="LRB02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4660065.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="LRB02_001871"
     CDS             complement(6352..6801)
                     /locus_tag="LRB02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4660066.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="LRB02_001872"
     CDS             complement(7246..7509)
                     /locus_tag="LRB02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL4660067.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /gene="rho"
                     /locus_tag="LRB02_001873"
     CDS             complement(7608..8903)
                     /gene="rho"
                     /locus_tag="LRB02_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL4660068.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="LRB02_001874"
     CDS             complement(8900..10171)
                     /locus_tag="LRB02_001874"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL4660069.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="LRB02_001875"
     CDS             complement(10336..11205)
                     /locus_tag="LRB02_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL4660070.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="LRB02_001876"
     CDS             complement(11497..13107)
                     /locus_tag="LRB02_001876"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL4660071.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="LRB02_001877"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="LRB02_001877"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL4660072.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="LRB02_001878"
     CDS             complement(15668..16060)
                     /locus_tag="LRB02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4660073.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3448 c         2766 g         5418 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtatcag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DAFFQY010000062        16116 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFFQY010000062 DAFFQY010000000
VERSION     DAFFQY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.062"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(152..838)
                     /locus_tag="LRB02_001879"
     CDS             complement(152..838)
                     /locus_tag="LRB02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4660074.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(974..1168)
                     /locus_tag="LRB02_001880"
     CDS             complement(974..1168)
                     /locus_tag="LRB02_001880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660075.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1218..1658)
                     /locus_tag="LRB02_001881"
     CDS             complement(1218..1658)
                     /locus_tag="LRB02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL4660076.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(1662..2507)
                     /locus_tag="LRB02_001882"
     CDS             complement(1662..2507)
                     /locus_tag="LRB02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL4660077.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2656..4074
                     /gene="pepV"
                     /locus_tag="LRB02_001883"
     CDS             2656..4074
                     /gene="pepV"
                     /locus_tag="LRB02_001883"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL4660078.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4150..5352)
                     /locus_tag="LRB02_001884"
     CDS             complement(4150..5352)
                     /locus_tag="LRB02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660079.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(5339..7126)
                     /locus_tag="LRB02_001885"
     CDS             complement(5339..7126)
                     /locus_tag="LRB02_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660080.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(7139..8092)
                     /locus_tag="LRB02_001886"
     CDS             complement(7139..8092)
                     /locus_tag="LRB02_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4660081.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(8277..8628)
                     /locus_tag="LRB02_001887"
                     /pseudo
     CDS             complement(8277..8628)
                     /locus_tag="LRB02_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
     gene            8828..9907
                     /gene="pepA"
                     /locus_tag="LRB02_001888"
     CDS             8828..9907
                     /gene="pepA"
                     /locus_tag="LRB02_001888"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL4660082.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            10050..10370
                     /locus_tag="LRB02_001889"
     CDS             10050..10370
                     /locus_tag="LRB02_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL4660083.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            10392..10856
                     /locus_tag="LRB02_001890"
     CDS             10392..10856
                     /locus_tag="LRB02_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4660084.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            11052..11657
                     /locus_tag="LRB02_001891"
     CDS             11052..11657
                     /locus_tag="LRB02_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL4660085.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(11696..12985)
                     /locus_tag="LRB02_001892"
     CDS             complement(11696..12985)
                     /locus_tag="LRB02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL4660086.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            13413..13892
                     /gene="rlmH"
                     /locus_tag="LRB02_001893"
     CDS             13413..13892
                     /gene="rlmH"
                     /locus_tag="LRB02_001893"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL4660087.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            14245..14559
                     /locus_tag="LRB02_001894"
     CDS             14245..14559
                     /locus_tag="LRB02_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4660088.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            14575..14961
                     /locus_tag="LRB02_001895"
     CDS             14575..14961
                     /locus_tag="LRB02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4660089.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            14990..>16116
                     /locus_tag="LRB02_001896"
     CDS             14990..>16116
                     /locus_tag="LRB02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL4660090.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         5269 a         2996 c         3132 g         4719 t
ORIGIN      
        1 tgatgcaggg cagctttttc acaacctctc aataaacggt gtgcaatcag ctgaccctcg
       61 gtattagctc aaaaaaaacg gcaaaatatg aggaactatt ttgccgttta tttctctaat
      121 actcaggtca aaacgctgat tccaaaacct cttatttgat gggtctgtcc tgaatatctt
      181 caaaataatc ggggtaatta tgaatcacgt attcttgctc ctcgataatc atatgtaaat
      241 gcatcgtgca aagaaatccg acttcatcaa agttatgatc aatcattgat tgaagcattg
      301 tctgatgttc atctaatact cgaccccagt ctaattctga aatcgtcaaa cgtaaccatc
      361 tgaaacggtt caagtgcgta ctataacttt ctagccaatc ccaaatttct cgttttccag
      421 caatttcata acaagttcgg tgaaaatcag tatccaaatc aaaaaaagct tttttatttt
      481 gagcgatcac tgcctgatcg gtttgttcta gtattttttc tagtttagct tgatcttgct
      541 tggtcatttt cgcactgcat tcttgcagga tcggtttttc caacttttcc cgcgtataac
      601 gggctaatga agcagatcgg atatcgattt ttgaaacata agtgccggac tgtggaatgg
      661 tttttacaag ctcttgattg cgcagttgga tcagtgcttc tcgaatcggt gtacgcccga
      721 tagctagttg ttcttctaac actttatcgg agattttact tccaggtgct aactcggaat
      781 aaatgatttg tttacgtata gattcataag cttgctgttg caaattattg ataaccattt
      841 aaccttcctt tctatgcaga tataatctat tataacataa atcgcttaca aatcgtgtgg
      901 gcataaaaac acagtctgca aaaaaagccc tgataggcat gcctattaga gcctcgaaaa
      961 gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg tttgacacga ccgccgattc
     1021 ttttagcaaa agcttcggat tcttttttgt ctttgaaaat gagcagattg tctaagtttt
     1081 tttcccgagt gcgacagcca gttttatcga tgtaaccgtg cattacttta acgacataca
     1141 tggggatcac ctgctttttt cttttcatta tacgaactac tcgacacaat acaagaaaaa
     1201 tgcttagacg gtatttatta agaatttaca tcatccaaag aagcgtacgc ttggaataac
     1261 tgttctaact ccggattgac tttgctcagc ttcactaatc gttgcgaatc agaaagtaaa
     1321 aaacagtgtt tactactgcc agtagttttt aattttttgt cttcagttcc cacaatttcg
     1381 tagctaaaat ccaaccgtgt tcccgtatac ttttggatag ttgggtggat gctcactttt
     1441 tctccgtaat ggatcatttc tttatagctg caatttactt ccagaacggg gatgatgatc
     1501 ccagattctt cgattttttt atagggaaac cctagatgta ccaataaggc tacccgagcc
     1561 tcttcaaacc agcggatata attgctgtga tgaataatcc ccatttgatc tgtttcataa
     1621 taaaaaggtt ctcgcacata acctgtaaat ttctccatga ctcatgcctc gttttccatt
     1681 gattttttac aaaaataatt catccacttc ggaagtgctt cgctgatttg tgtcggcaaa
     1741 acaatatatt gttcttttgc cagctggtct ccaatgaaac tatgaagata aacagctgct
     1801 gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc ctgtgatcat cccagctaaa
     1861 gtgtcaccca tgccgcctgt tgccatagca gcagaaccta aaggattggt gtagaagtgc
     1921 tggtgaccat aaatttttgt ctcatgactt ttcaaaacga gcacagcacc gatttcctgt
     1981 tgcttctttt tgttttcttt atcccgttgt tcttgtatcg gtagagaact caaccgttgc
     2041 cactccattt gatgaggtgt aagtacagtt tgttcgggat agggtagcaa aagatgatta
     2101 gcaacaagta aacttaaagc agagccatca atcaccagcc attgttcttt tgattgtttt
     2161 tctagtactt gtttgaaacg ttccaaactc ttagaagaag tacccaatcc gggaccaatc
     2221 aagagtacat ctgcttgctt tattacggaa agaaactggt cagtatccgt ccaatctaca
     2281 atcattgctt ctggcagatg actgtgaaga gcactccgat ttttttcatc agttactaca
     2341 gtcgtgagac ccgctccact gtgtacggca gctaaagcac tcataatgat tgctccgcca
     2401 tattgttcat tgccgccaat caaaacgata cgtccaaatg ttcctttatg tgaaatcttt
     2461 ggacgaggtg tgatgactct tttcactagt tgttcgttta tcagcatcga aaaacctcct
     2521 tttactcatt atagcgtata ttttttttag agaaaaacag ataagaaaag gaattgtcag
     2581 attttttgaa ctattttttt ctgtaatttg tggtatcatg aacacgaaga cttaaataag
     2641 tggaggatta actttatgac aattgattgg caaaaagaag tagaagcacg taaagaagac
     2701 ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg agcgcgatga ttcaaaagta
     2761 acaccagatg caccatttgg acctggacct cgcgacgcat tgaaacatat gttagcttat
     2821 ggcgaacgtg atggatttaa agtaaaaaat gtcgataact atgcagggca tattgatctt
     2881 ggcgaaggag acgaaacgct agggatattt ggacacatgg atgtcgttcc tgcaggagat
     2941 ggctgggaca cagatcctta tgaaccagta atcaaggatg gaaagatctt tgcacgcgga
     3001 tcaagcgatg acaaaggtcc aagtatggca gcttactatg cgatgaagat cattaaagaa
     3061 cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg gcagcgatga agaaagcggc
     3121 tggggcgata tggcttatta tttcgaacat gaagaagaac cagattttgg tttctcacca
     3181 gatgctgaat tcccaatcat caatggtgaa aaaggaaatg tttctttagc tttacgcttc
     3241 aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg tttccggact tcgcgaaaat
     3301 atggtgccag gaacagcaac tgcagcactt caagtaccaa gtgcagatgc agctatcgcg
     3361 atggaagaag cattttacca attcatcgaa gcaaatccag taagcggaac gattgaagcg
     3421 gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg cacatggtgc aagcccacaa
     3481 tctggtatca atgcaggttc tttcttggca ttgttcttag ataactatga attcctagga
     3541 tctgctaaac aatttatcca tgtagcagct gcttatgtcc atgaagattt ttatggagaa
     3601 aaactaggag tcgcttatga agatgaaaaa atgggcaaat tgacaatgaa tgctggacta
     3661 tttgcttttg aagaaaatgg tacagaggaa gcgaacttca tcaatatgaa tttccgtttt
     3721 ccaaaaggtg taacagtaga cggattgcaa agtgacatcg aacaaacagt ggggcaagaa
     3781 ggcgcaacag ttactcgagg tgctcgcgta atggaaccac attacgtacc aatggatgat
     3841 ccattggtgg ctacattatt gcaagtctat gaagaccaca ctggagaaaa aggctacgaa
     3901 caaatcatcg gtggcggaac ttacggacgt ttgttgaaac gtggcgttgc ttatggagcg
     3961 atgttcccag gatatactga tacaatgcat caagcaaacg aatttatgag tcttgatgac
     4021 ttgttcagag caacagcaat ctatgcagat gccatctatc gtttggcgaa ataaaaatac
     4081 taaaaaactc cggctatctc tgacagctgg agtttttatg tttaaaaatg aattagtaca
     4141 tcggtattct taaaagtaat ttagttcgtc ttttgatttc tcaggaggga tcaaaaaggc
     4201 gaaagcacta agtatagcaa aacttgtgat gatttgcgta cgatacgtaa accaataatg
     4261 aatctgtgag tgattagcta aaatattgta ccaaatatac ggcgttacag cagctaaaac
     4321 aaacaaaact agaagttgga acggacgcca gctatttcgt ttacgataag caaaatagag
     4381 aaaaacaaga caagtaatca atagcaataa tattgctact tttggataca tcaatctaat
     4441 attgcttttc agcatctcta atcgatgcaa cgggtatttg tcgtttcctt ccgttcgaaa
     4501 gaggatttgg ttgattgcat cttttacaat gtttttgttc aatatgatcg atgataggat
     4561 ccatttagcc acccatgtca aaccatagcc taatccccaa aagataccgg taaaaacaaa
     4621 actgcctact tgtttgctca atgaactgcg tttatccatt aaatacttat ttgttacata
     4681 ataaataatg atcacaggta tcccccaaga gatgaccggt gttgtcaaca agtcaaaaaa
     4741 gttagctaaa gaaccaatga caaagaaata caagaataat tgttggttgg tgttggagag
     4801 ttgtggtttt gttaaaaata gtaatacaga caataaagtc aaaatccaaa tatttgaaaa
     4861 ttgcatactg aagaaaaagg ttgcagcatt tcctatagca agcgaaatca acaaggctgt
     4921 cgttaaatag atgtcgctac acttggttaa aaagtagctc gtcaatccaa tcagcagaaa
     4981 aagaatggct gcgtaaatca ttcgaatact ccccaaactc atccctagca gtaaaggtcg
     5041 taaaaaaagc tgataaccat gccaataacg gggataatcc gccatagaca tcgcgttctc
     5101 caaggagttc aattcaggat ctgcttttgt ttttcgtatc atcaaatgat cagtaaaatt
     5161 atctaaccga gtgcctagca tatttccttg attgatagaa ggatacattc cttctttttc
     5221 aaccatctgt agagattttt ctgcattcga ttcaatagag tgctgcggaa tgaaaaaagt
     5281 aattgtcagt agtacgcaat agctagcgat caagagaaca aaagcaaaaa atatcttttt
     5341 aaatagtttc atgagtatct cctatatctg ttttcatttt ttctctttta tctagtaaaa
     5401 aaggagagag gatataaaca ggtagaccta atgctaaaac aaacacatac cgtaaactaa
     5461 aagccacagg agtagcaatc ataatagtag caaaaacaag cataccggga agaaaaagga
     5521 gtaaataccg atattttttc ttaaagattg ctaaaagtgc actgccgata gctaaccaca
     5581 gtaatgtgcc ggaaccaaag aaatcacgag catcgatgat ttttttcatt cctgacaatc
     5641 cagtaagatg ttcaatcaaa tctacttgct tcataccata attattcgtg tttacacgag
     5701 tatctaaaaa gccgtaacta ttttttgtac caggtaccca gaacccgaac gtttctaaaa
     5761 tataagcatc tgtatagatt tttatattac ttggtaaatt tcttgcccat agtgatagaa
     5821 attctttttt atgtgtattc aaaaactcat tgttaaaatt tttatgccac ttgattgagt
     5881 ctgcaagact aggagaatac gcttcataat cttttatcgg gagtagctga ttcatgaatg
     5941 ctttatcttt cttagacaag tcaccatcca taacaatagt tctagaaatt tgctgcaaag
     6001 gaacacctaa ttgttcagca gtttctgtag gaatagcata acgagccatc aacgggtgga
     6061 tgagtagtcc agcaatcact gtataaccca gcaccacttg aaagaatgct tgataccttt
     6121 tttgtttcaa aataatcaag ccgcaaatca aacctgtgcc ggttactaca taaatcccat
     6181 tatttcttag ataggtggtc agtaaagcaa ggatgattac gtaaatctga ttgagaatcg
     6241 ttggcttttc tcgttgaaaa ataaagtcaa aaaccgctaa ggaaaataaa aagcaagcga
     6301 tagagaacaa agtatctttt tgcatgttca aggcgtagat tggaaaaacc ggtgcaaatg
     6361 caaaatagca taaactaatg agtaccggaa aaaaatggat gtttcttttt aacagccagc
     6421 aaagagaata acttatcgct gaagctgtta tcaatgtttg aaaaatcgaa aataaaaata
     6481 caccgtgatt gatcgtatca gttggtaaat catgaacaaa tagcttgacc agtaaggtga
     6541 ataagactgg atgatgattg cttataggaa catcacccat agattgggcg accgaactta
     6601 acgaatcagg caaaaccact ccaggataca atgccaacag ataaggaagc caacacagaa
     6661 ctaaaaagac agtaaagaat aaaaaatatt ttcttgaaga gaaagcctgt gtgttttgag
     6721 cagtaaaaaa tgtttccagt tttggcattg ataaataaat cgattgtaaa aataaaaaag
     6781 aatacacaaa aatggagaaa taaacaagaa agtaaaaaat acttccatca gcaatcgttg
     6841 tctttgtata gtttgctgag acatactgaa attgtatctg tctgccaata gaaagtgtaa
     6901 gagcaaaagc tgcagaaaaa aacaatcggg caatagctgc aactttgttt tttattgaaa
     6961 acaactctat tccatattgc ttaaaagcaa gtagcaaaac catggctaaa ccaatgatta
     7021 ctgttggatt caaagttcct agaccataag aagtaaaaaa agcaaaacct gacgcaaata
     7081 aagttgcaaa aaaagtagga aatagattct cctttttctt atgcataaaa aaggcctcct
     7141 acttcatttc tttattttga tattcgtggt agacatggta taaattcaat tcatattctt
     7201 tcttggcatt tgtgacgacc gtatccaaaa tcaaccctgt tactaatgaa agaagggcta
     7261 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     7321 tatattcacg aatcacagga atccccatga ccaatccaaa taaaaagaag aagatggtcc
     7381 agatcccaaa gaaaagcaaa ggtttataat ctttgcacat tttgatgatc atcatgatca
     7441 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct accttcagga cgatcgcgat
     7501 aatcgattgg aatctcaacg aacttgaatt ttttatctaa agcatgaatc gtcaattctg
     7561 tttcgatttg aaaaccagaa ctcataatag ggaagctttt gacaaaaatt cgattgaatc
     7621 ctcgatagcc ggtcatgaca tcgcgaattt ttgttttata taagtaagta attgtatttt
     7681 taaccagatt attcccaaaa tcatggaaag gacgcttatt ttcatcaaaa taggttccat
     7741 ttgatagacg atcgccaatg accatatctg cttcgccaga acgtaatttc tcaagcaaac
     7801 catgtgcagc ttctgcagga taagtatcat caccatctac catcaaataa taatcggcat
     7861 caatatcaaa aaacatttga cggattacat tcccttttcc ttgacgtggt tctttcttta
     7921 cgattgctcc gccttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     7981 cataaatatc tgcttctggt aattcccttt taaaatcttc aataacagtt gaaattgtcg
     8041 cctcttcgtt ataacaagga atgagcacgg caattttttc acgtgattcc atttgtcact
     8101 ctccttatat aataaaacta atcttatttc aataataccg tataataact acaaatacta
     8161 gctattccaa gaggaaatag ccctattttt ataaaaatca cccactataa aagaaggata
     8221 ggatacctct cttttagata gtgggtgcgt attcgttatt tcattttttt gacgtattaa
     8281 tctattgcgc ccttttttct tgaaatgtct aatacggttc ctgtatgagc atctgctaaa
     8341 aattcataat aaacagtttc gccatcttct atccgggaaa gcccaccccg ataagcactc
     8401 gaccgaacgg cgaatttccg aataggttct ttttcaaaag agatccaagc accttcgatt
     8461 gcgccttctt ttaaaaaaga tgttttgata ttttcaagca cttggtctgc tgataacact
     8521 ttttttgcga tgataccaaa tcgttgaagc taccccactc aataatccta gaccgatccc
     8581 aacggctaac ccgcctttaa aatagcttaa ttcgctttct tcatacatgt ggatatacct
     8641 ccttcttctc acttgctact ctttgtgttt attttaccac actctcatat tttctttgaa
     8701 ataaataacc gctaccagaa aaaataagct ataataaata cgatcggtct gcagaaaaaa
     8761 tcatatttta tccgcagatc aatataagtc tgagaggagt ttagtctgaa atttagacga
     8821 atagcatatg gaagaaaaaa catttcaacg aatcaaagag ttaacagaat tacaaggaac
     8881 aagcggtttt gaacaagacg ttcgcgccta tatggaaagt catatggaac cgctggtaga
     8941 tgagttacag ttagatggtt tgggcggcat ttttgggctg cgccaccatg aagaagcgga
     9001 tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc ggattcatgc tgacacaaat
     9061 ccaagaaaac gggcttttta aagtcgtacc tctaggcgga tggaaccctt acgttgtttc
     9121 tgctcaacgt tttacactta aaacctctac tggtaagaat tatccatgta tttcttcttc
     9181 cgttccccca catttattac gaggaacaag cggacaaaaa tcagtagaag taacagatat
     9241 tttgttcgat gctgggtttg aatcaagaga agaggcaatg agttacggtg ttcttccagg
     9301 agacacaatc gttccttacg ctgaaacgat caaaacagcc aacgggaaga atatcatcag
     9361 caaatcgtgg gacaaccgct atggctgtac aatggtatta gaagctttag aagcgttaca
     9421 aaatgaaaca ttaggtcata cattgattgc aggcgcgaat gtccaagaag aagtaggctt
     9481 gcgtggttct aaagcttctg tcaataaatt caagccagat ctgttctttg cagtagactg
     9541 ctctgcagca gatgacacag tgacgaaaaa tggcacattc ggacatttag gagaaggtac
     9601 attgatgcgt atccaagatc caggtttgat catgcttcct cgtttgcgtg agtacttgct
     9661 agatatcgct gaaacaaaca acatccctta tcaatacttt gtttcaaaag gcggtacaga
     9721 tgcaggtgct gctcatacac aaaatgaagg aatcccaagt acagtgattg gcgtagtggg
     9781 acgttatatc catacgcatc aaacgatgtt cagtattcgc gattttgaag ctgcacgaga
     9841 aatgttgatt caaacattga aaggtttaga taaatcaaca gtgaacacga tcgtatacgg
     9901 gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc aaccttttgt cagatttttt
     9961 ttataaaaag aggatcaatg tttgttagtt cgaaagaaag gtgtataatt taattgaaaa
    10021 atacataaac agaaagtaga ggaaaaagaa tgattatccc aaatagttta gaagaattag
    10081 caggctacgt agaaactgga aaaagtgtat ttttctttac tgccggctgg tgcggagact
    10141 gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga cttttctgat tggcaattta
    10201 ttgaagtaga ccgtgacaag tacatagatg tagccgctga atggaacatt tttggtatcc
    10261 caagttttgt cgtgatccaa gacggaaaag aattaggtcg tctagtgaat aaagatcgta
    10321 aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat aaaaggctga catctgttag
    10381 gaggggtcaa gatgaatcaa gaatataaga acattttagt aggtattgac ggtagtgaac
    10441 aagcacttgc tgctttcaaa aaagcgttag aagtggctcg ccgtaataat gggaaagtat
    10501 atgtagcaaa cgtaatcgat cagcaatttt ataattttat gggctatgct cctattgatc
    10561 aaaatctagt cgatcagcaa actgaaacag ccaagcaaat gatcgaagaa tgtaaagaat
    10621 atggaaaatc tgttgattac acaaatatag aaggaattat tgcatacggc tctgttcgtg
    10681 aatccatggc agtcaaattg ccaaaaaaat atgcaatcga tctaatcatg gttgggcagt
    10741 ctggactaaa tgcagtcgaa cgctttatga caggaagtgt agccagctac gtcatacgtc
    10801 attcaccttg tgatgtcttg atcgtttctg acgaagaaga acagccaagt aattaagcaa
    10861 gtggaacttg tataaccttt ttaatcaaac agtaaaggga ctgtgacaaa agtcttgtca
    10921 cagtcccttg ttacgaataa atggtgttcc agaagaagct tcttcggaaa taaagcatga
    10981 aaaaaatctc atgcagtgaa tgcaattttt tgttaagata aatctcagca aaggataatg
    11041 gaggaaagaa catgattttt gcttataaca aagcacacgt cggagacact ttgatggtta
    11101 tcatagccga cgataaagga acagaaaaca ctgtcgaacg taaatggaat gttgctcgga
    11161 tcaaagatga acaaggtcaa gttgttgctt ggaacttttt ccatatctcc gatcatctaa
    11221 caattgaagg gaacggccaa gtaagcttaa cagatgaaga attagcgaaa ttgaatcgat
    11281 tgattaaaga agcaggattc gaagaacagc ttgaagctga tcatgaaccg aagatcgttg
    11341 tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga ccatttatcg attactgaga
    11401 cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg tgcaccaaat atcgaagctg
    11461 gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc agatggaatg atgatctggc
    11521 caggaacttt gcgaggggta gccagctatg gaatgatttg ttctgctcgt gaattgcatt
    11581 tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc tgaggatgcc gtagtcggag
    11641 aagtatttcc aaaataaaga ggttgtgaca agtctttgtc acaacctctt tattattaat
    11701 tttcactatt ttgattttgt tttaatgccg attgatcgat cgtcaattca acagtagttg
    11761 ttttttcttt cgagccttca tagtaagtga ttttgacaga atctcccact tttttcttat
    11821 acaaagcaga ttgaagttct acaccagaag aaacttcttt gtcatcgatc ttagtaatga
    11881 cgtcgtattg tttcaaccca gctttttctg ccggtgtagc tgtttggaca tttgtcacga
    11941 tgacaccgtt agttacagat gaaggaatct tcaagatttg ttcttgttgc tgagtagaaa
    12001 cggcagataa gtcgaccatt gtgataccta gagctggacg tgtcactttt ccgtcttttt
    12061 ccagttggtt gatgatattc actacatcgt tacttgggat ggcaaatccc attccttcta
    12121 cgcttacatt agaagtcgat tcagaagtac ttgcgatctt acttgagttg ataccgataa
    12181 cttgtccttc gatattcact aaaggaccac cagaatttcc cgggttgatc gctgcatctg
    12241 tttggatcgc attgatattg actgtttcgt tcgattcatt cgtacttgtt acttgtcggt
    12301 tcaaggaaga aatgattcct gaagtgacag aattcgcata ttcagatcct aatggggaac
    12361 caatcgcaat cgcaggttca ccaaccttca aagcacttga gtccccaaaa gaggcaaccg
    12421 tttcgacttt gtctgaattg atcttcaaca ccgctaaatc tgaataggca tctgttccaa
    12481 ctaattcagc ttttaccttc gtaccgtctt tcatcatgac ttccaaacct tgctgtccat
    12541 ctactacgtg attgtttgtg actacataag cagtattccc gtcttttttg tagattacgc
    12601 cgcttccttc actataagct tccaaattac tatcatctga gctgctactt tcttcttgct
    12661 gcccgaataa tccgccaaaa ccgcttgatt ggttttgact ttgtaagttg ataacagaaa
    12721 cgacagcgtc ttgaacttta tctaccgctt tggtgatatc agaatctaca ttgactttga
    12781 cattttccac aactgtttca ccggcagaat tttgattgcc gcttgtagca gtagaactat
    12841 tgttgccagt gcccatcgct gcataaaaaa tgccagctgt cagtagtcct ccaacgatcc
    12901 ctccaacaag ccccagaccc aacttacgcc agaggctatt atttttattt tttttcatct
    12961 ttggtgtcac attttttcta tccatgtgtt cctcctccaa ttacattcat ttatctactg
    13021 ttagtatagt cttatttgtt gactttagtc tactttgaga ctttgaactt tttatgaaaa
    13081 actttaggaa aaatcattgt tttcagatga aaatatttga ttttcttttt ccacagatat
    13141 tgtgtaaaac tacgcggata ggattggaaa aataagggat aaaacatatc cacagcctgt
    13201 gtataaagtg gataaattct tataaaacgt atgtttgcct aatgaatgtg atgaaataag
    13261 gtaaaacaac gctgttttct gtggataaac ctgtggatgt tgtgcataac tgttggggaa
    13321 atctttttaa caaaaatagt caaaataaac atatagacga aaaaattgat tataataaac
    13381 aaagtgagcg tgaagatcaa aggagagaaa acatgaatat caaaattatt tcagtaggaa
    13441 aattaaaaga aaaatacttg atccaaggta tcaatgagta tgtcaaacgg ctgaatgcct
    13501 atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc cccggagaat ttaagcgaag
    13561 cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat cttagcgaaa atcaaagaac
    13621 aagaatacgt ctatggctta gcaattgaag ggaaaaatcc cacaagcgaa tcttttgcta
    13681 aacaaatcga ccaattaggt atccaaggaa aaagccacct tgtttttgtt atcggcggtt
    13741 ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc tcagatctct tttggcaaaa
    13801 tgacgtatcc acatcaattg atgcgcctga tcctcgtcga gcaaatctac cgtgccttcc
    13861 ggatcaacgc gggggctcca taccacaaat aatgatattt tttaagagaa aaatagcata
    13921 aaaatctagt tatccgcata aaaactggac ttatcacact ttatcaaggt caaaaccact
    13981 caatttacta ctaatttact acttatgaat gagctttgat acgacgattt atccttgaaa
    14041 agtgaagata taaagatact tccaataaaa tttgaatatt taataggtag acacttcaaa
    14101 aaatgaggtg tctatttttt tacccgattt tgaaaggaag tgaacttatg aaaacaaaaa
    14161 atcaagaatc aaaaggtcgt tccccactct ttaagaccat caaacattca ttcagccaat
    14221 aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg tgattcccaa catggaaaaa
    14281 acattcggca atttagaatt tgctggcgag gataaagtcg ttcagcgaag aatcaacgga
    14341 cggctaactg tcttatcaag aagctataat ctctattctg atgttcaaag agcagatgat
    14401 attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt tcggctttga ggaacgtgtg
    14461 aagttagtca atccacgtat taccgcagag ggctacaaaa tcggcactcg tggttttaca
    14521 aattaccttt tacatgctga cgacatgata aaagaataaa gaaagagagg aaaaatgatg
    14581 agattagcaa atggcattgt attagataaa gacacgactt ttggagaatt gaaattctct
    14641 gctctacgtc gtgaagtgag aatccaaaat gaagacgggt cggtttcaga tgaaatcaag
    14701 gaacgtacct atgacttaaa atccaaagga caaggacgca tgattcaagt aagtattcct
    14761 gccagcgtgc ctttgaaaga gtttgattat aacgcacggg tggaacttat caatcccatt
    14821 gcggacaccg ttgctactgc cacctatcaa ggagcagatg ttgactggta tatcaaggca
    14881 gacgatattg tgctgacaaa ggattctagt tcattcaaag ctcaaccaca agcaaagaaa
    14941 gaaccgacac aagacaaata gtcgctaggt agaaaggaga ctttttcgca tgaaacagcg
    15001 tggtaaaagg attcgcccat ctggtaaaga tttagtcttt cattttacga tagcgtcact
    15061 cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg aagacaatcc agcagatcaa
    15121 ctggcaggat tttaacctat cacaagcaga taagattgac attccctatt taattatcag
    15181 tttcagtgtc gcaattctta tctgcttgct ggtagcgttt gtattcaaac gggttcgcta
    15241 tgatacggtt aaacaacttt accaccgtca aaaactggca aagatgatac ttgaaaacaa
    15301 gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt aaagatagtg ctggtcgtac
    15361 aaaggaaaag ataacctact tccctaaaat gtattatcga cttaaaaatg gcttgataca
    15421 gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa ctcttacact tggaaaagaa
    15481 attagagagt ggcttgtact gtgagctgac ggataaagag ttaaaggatt cctatgtgga
    15541 atatactttg ctctatgaca ccatagccag tcgtatttct attgatgaag tagaagctaa
    15601 agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa tatgataagc tccctcatat
    15661 gttgattgct ggtggtacag gtggcggtaa aacttacttt atactgacac tgattgaagc
    15721 cttgcttcat acagattcaa aactgtatat tcttgacccg aaaaatgctg accttgcgga
    15781 cttaggttct gtgatggcaa atgtctacta tagaaaagaa gacttgcttt cttgcattga
    15841 aacattctat gaagaaatga tgaaacgtag tgaggaaatg aagcagatga agaactataa
    15901 gactggcaaa aattatgctt acttaggtct cccggcacac ttcttaatct ttgatgaata
    15961 cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc gcagttatga ataagctgaa
    16021 acagattgtc atgttaggtc gtcaagctgg cttctttcta atactggctt gtcaacgtcc
    16081 agacgcaaaa tatttaggcg acggaatccg tgatca
//
LOCUS       DAFFQY010000063        15851 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFFQY010000063 DAFFQY010000000
VERSION     DAFFQY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.063"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            292..1431
                     /locus_tag="LRB02_001897"
     CDS             292..1431
                     /locus_tag="LRB02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL4660091.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="LRB02_001898"
     CDS             complement(1498..2073)
                     /locus_tag="LRB02_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4660092.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="LRB02_001899"
     CDS             complement(2078..3052)
                     /locus_tag="LRB02_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL4660093.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4302
                     /locus_tag="LRB02_001900"
                     /pseudo
     CDS             3335..4302
                     /locus_tag="LRB02_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            4415..4693
                     /locus_tag="LRB02_001901"
     CDS             4415..4693
                     /locus_tag="LRB02_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL4660094.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="LRB02_001902"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="LRB02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL4660095.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            5150..5905
                     /locus_tag="LRB02_001903"
     CDS             5150..5905
                     /locus_tag="LRB02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4660096.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="LRB02_001904"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="LRB02_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL4660097.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="LRB02_001905"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="LRB02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL4660098.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="LRB02_001906"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="LRB02_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL4660099.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="LRB02_001907"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="LRB02_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL4660100.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="LRB02_001908"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="LRB02_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL4660101.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="LRB02_001909"
     CDS             10642..11184
                     /locus_tag="LRB02_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL4660102.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            11207..12763
                     /locus_tag="LRB02_001910"
     CDS             11207..12763
                     /locus_tag="LRB02_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL4660103.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="LRB02_001911"
     CDS             12777..13679
                     /locus_tag="LRB02_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL4660104.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="LRB02_001912"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="LRB02_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL4660105.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="LRB02_001913"
     CDS             15122..15544
                     /locus_tag="LRB02_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL4660106.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5263 a         2840 c         3419 g         4329 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attgcattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
     2281 tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga tcattgatga
     2341 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
     2581 taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt ttcccaatat
     2641 ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca atgactgatg
     2701 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
     2761 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
     2821 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
     2881 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
     2941 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
     3181 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagtt
     3301 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
     3361 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
     3481 ccctgctgca gtcacgtcag tagaaaaaaa cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa actagctggt
     3601 cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca tgattaccga
     3661 agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga acagccgaat
     3841 gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat ggcaagtggt
     3901 gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt ttctgtttat
     3961 gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat ggtcatgaga
     4021 tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc atcagctacg
     4081 agacagcttg gcagcaacca tctgccttta ttggtcattc atggtgaaag agatcatttt
     4141 gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa agcactgctg
     4201 cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatgaatt aattttctct tcttttttgt
     4321 aagtaattcg aggataaata attgtttata gatcaggaga ctgctaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag aaaaaaatcg
     4441 tcttgcttta ttcaatgatg aacaacaaga gatcggagaa atgacatggt cagatgcggg
     4501 cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg gtcaaaagtt
     4561 agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga aaaagatcat
     4621 tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc aagacgtctt
     4681 acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat tccatttata
     4741 tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat atttatcttc
     5041 gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgagacgagt
     5161 gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt tgctgcttca
     5221 cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg aaaaagcaga
     5281 ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat atgatatttt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt ttttacagtc
     5401 tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct ttgctgtacg
     5461 agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt tgtctcctgt
     5521 aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga agacagcagg
     5581 taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag ctgaacaaca
     5641 attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag atatccaaca
     5701 gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg gcgtatttga
     5761 aactgcacgc aagttgagcc gtcagcgagt gacacttcga tggtatcctg aaagtggtca
     5821 tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc tgaaggttta
     6001 gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa accaatcttg
     6121 atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg agacaccgta
     6241 gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga ggggaaagta
     6301 gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc ttattcagaa
     6361 gaagaaatac aagaaacgga tctttacggc gttgttatcc cgaaagatag aaagctcaac
     6421 caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag tattgtgctg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt cgtggctaat
     6601 ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt tccagatcaa
     6661 atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat tgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt agataacggt
     6781 aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca aggcagtcaa
     6841 ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg agtgattccg
     6901 atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt tccaaggctg
     6961 accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca tgatattttc
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat ttcttttgag
     7201 gatcgcgaag caaaaatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa tgaaacggta
     7321 gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctacg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg ggaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gagggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacagaa agtgcgagta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtagatt ttgaacttgt ttccgcagaa gaggttacag cgttggaacg gccaaaaaca
     8101 cgaaatggaa aatcaggaaa acgcgaagga aaacaagcag gaaaacgccc agaaagaaat
     8161 ggcaagaaga acaaacgata tagcgatagt aagcaaacgt tcggtaagaa aaaagggaag
     8221 aagccatttt acaaaggtgt taaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaatta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaaccga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctaaaagat ggatttattc gcgtccgcaa cggagaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaaa aaacaaatca tccggcttga aaatgaattg
     8581 aaaaatactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcgaa agggaaaaaa tcttatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc ataggttttt tcttagatca ggagcaccgt gtataaagaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgttccaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatcgg tttagtaaca aaaatcgttt tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tatttggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccat atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatgg taataagctt aggctggttc tcattgccgt tagcaattcc
     9661 attagagatg gtctggatcg cattctcctt gtttatcgga agcatccaag catttgtctt
     9721 cgttacttta tctatggttt atatgagcca taagattgaa gtggaagaat aatattcgtt
     9781 ttaaaaacaa actactattt atctaattta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gtcgaagctg ttcctatctt aggggtagtt gtcgccttga
    10021 ttctagtttt tggtgtttaa ttgaatcagt attaaggcag aaacaatttg gtttgttact
    10081 gcttttttac atgaaaacta gaagtacttc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggaaa tgtcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc attttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttggattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gtaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg atgttgcaga actatctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcacga atcattaatc aatcaatata
    10621 tcgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aataaagcag aagaaattta tcaagatctc
    10741 gtaagcttac gtcaaatcta tgaacaagta cctggattag gtaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgttagtgg ctatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gcgtataacc gaatggatga tcttttgttc
    10921 atgatcgaag aatatgaacg tcgatatgat gaacatcaag gtttgctgtt gggaagtgtc
    10981 acaacagcag ttccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatgaact atcaatcagt cgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagctaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaat
    11161 atgcgtaatc aattaaatag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaatggc tcatacggaa tggcgcaaaa
    11401 cttagaatca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gaaagatcat ggaagttcca gttggagagg ccttgattgg
    11521 acgggtagtt aatccgctag gtcagccaat tgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcaa tggcaccagg cgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca ggtctaaaag caatcgacgc tcttgtacca atcggacgcg gccaacgtga
    11701 attagttatc ggtgaccgta aaacagggaa aacttccatt gcgatcgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag cgccgttgct ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagag ttcatgtaca acgggaaaca tgtcttgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag gtcgtgaagc
    12061 ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag caaaattaag
    12121 tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat ctgtatctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact tggatgcagc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaattgca
    12481 tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac atggattctt
    12541 agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgttgctca
    13021 gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt ttagtcggtg
    13081 gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga c
//
LOCUS       DAFFQY010000064        15687 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFFQY010000064 DAFFQY010000000
VERSION     DAFFQY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.064"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            68..751
                     /locus_tag="LRB02_001914"
     CDS             68..751
                     /locus_tag="LRB02_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL4660107.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="LRB02_001915"
     CDS             753..1589
                     /locus_tag="LRB02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL4660108.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="LRB02_001916"
     CDS             1600..3354
                     /locus_tag="LRB02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4660109.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="LRB02_001917"
     CDS             complement(3630..3971)
                     /locus_tag="LRB02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4660110.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="LRB02_001918"
     CDS             complement(4039..6210)
                     /locus_tag="LRB02_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL4660111.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNRTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="LRB02_001919"
     CDS             6379..7278
                     /locus_tag="LRB02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4660112.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="LRB02_001920"
     CDS             complement(7349..8206)
                     /locus_tag="LRB02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4660113.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="LRB02_001921"
     CDS             complement(8191..10893)
                     /locus_tag="LRB02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL4660114.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="LRB02_001922"
     CDS             complement(10906..12195)
                     /locus_tag="LRB02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL4660115.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="LRB02_001923"
     CDS             12345..13391
                     /locus_tag="LRB02_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4660116.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="LRB02_001924"
     CDS             13393..15546
                     /locus_tag="LRB02_001924"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL4660117.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4455 a         3281 c         2886 g         5065 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc tatttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DAFFQY010000065        15520 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFFQY010000065 DAFFQY010000000
VERSION     DAFFQY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.065"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(363..1525)
                     /locus_tag="LRB02_001925"
     CDS             complement(join(363..1249,1249..1525))
                     /locus_tag="LRB02_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4660118.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            1734..2108
                     /locus_tag="LRB02_001926"
     CDS             1734..2108
                     /locus_tag="LRB02_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4660119.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(2222..2851)
                     /locus_tag="LRB02_001927"
     CDS             complement(2222..2851)
                     /locus_tag="LRB02_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4660120.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(2985..3057)
                     /locus_tag="LRB02_001928"
     tRNA            complement(2985..3057)
                     /locus_tag="LRB02_001928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3022..3024),aa:Thr,seq:ggt)
     gene            3271..3344
                     /locus_tag="LRB02_001929"
     tRNA            3271..3344
                     /locus_tag="LRB02_001929"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3305..3307,aa:Asn,seq:gtt)
     gene            complement(3482..3646)
                     /locus_tag="LRB02_001930"
     CDS             complement(3482..3646)
                     /locus_tag="LRB02_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660121.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            3941..5269
                     /locus_tag="LRB02_001931"
     CDS             3941..5269
                     /locus_tag="LRB02_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL4660122.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            5471..6304
                     /locus_tag="LRB02_001932"
     CDS             5471..6304
                     /locus_tag="LRB02_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL4660123.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            6320..7057
                     /locus_tag="LRB02_001933"
     CDS             6320..7057
                     /locus_tag="LRB02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL4660124.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(7212..8180)
                     /locus_tag="LRB02_001934"
     CDS             complement(7212..8180)
                     /locus_tag="LRB02_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL4660125.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            8412..9245
                     /locus_tag="LRB02_001935"
     CDS             8412..9245
                     /locus_tag="LRB02_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL4660126.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            9279..10118
                     /locus_tag="LRB02_001936"
     CDS             9279..10118
                     /locus_tag="LRB02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4660127.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            10144..10704
                     /locus_tag="LRB02_001937"
     CDS             10144..10704
                     /locus_tag="LRB02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4660128.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            10903..12624
                     /locus_tag="LRB02_001938"
     CDS             10903..12624
                     /locus_tag="LRB02_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL4660129.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            12789..13931
                     /locus_tag="LRB02_001939"
     CDS             12789..13931
                     /locus_tag="LRB02_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4660130.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            13947..15158
                     /gene="thiI"
                     /locus_tag="LRB02_001940"
     CDS             13947..15158
                     /gene="thiI"
                     /locus_tag="LRB02_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL4660131.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         5185 a         2614 c         3111 g         4610 t
ORIGIN      
        1 aaagtagatg taagaaatat catactgtag acaaaagtat cagtttcctc tttactaaga
       61 gtgtagactg atacttttgt ctatttttgt ataataaaaa atatgaagac atatgctccc
      121 actgctaagt gaccaatttt ttcagactca tttcggcaaa aacaagcatc gtatgcacct
      181 gatttttttc taagcctcga tacctgatgc agcgcattcc atgctttttc tcattggaat
      241 ggcaacagtt tttttgacaa attttataag gtgcagaact tctttccgta tgctattccg
      301 attgtccttg acaatgagcc tcctcggatg tgacgatctt cggcaggagc taacagttaa
      361 agttagactg cctcgctagc gttagcacat ccgagctcat tatcaaggat tcgctgcgcc
      421 aatctctggt tacggtaacc aaccggtgtc tcgtagccaa gtgtaccgtg caaccgaagg
      481 tggttccacc aattgacata gtcaaataac tccaaatcca attgttgtaa ggtttcaaat
      541 gtgtattgat agacaaattc tactttcaac gacttataag ttgattcagc tacggcatta
      601 tcaaaaggac agcctttatg actcaatgat cgattgatgt caaaagttgt taataattca
      661 tcaatagctt ggttatcaaa ctcttttcca cgatcagtat gaaaaatctc aacctctgtc
      721 agaggttgtt tgatacggct aaatgctttt tttactagaa cggcatcttt atgttctcca
      781 caagaatagc cgagaatttc tcgattgaac agatccaaaa tgaaacagac ataattccat
      841 tttttcccga ctcgtacata agtcaagtct gttacgatcg cttctaatgg gttgtctctt
      901 aagaatttac gattcaatac gtttgtcgtt ttggcttcat tgcaagtaga atgatgtact
      961 ttaaaataag caacagtata gctcgatttt aatcctctat ttttcatgat tctactaatt
     1021 tttcgtcggc tgatctgaat gcctcgtttt gataaggctt tttttatttt tcttgagccg
     1081 taggcctttc ggctgcggat aaattcttca gcgactactt cttcaagttc tgattcgtct
     1141 ttctttggtt ttgattgata ataataggac tgacgtgata gacctaatat tctgcacatc
     1201 gctgatatag ggtaaagatg cttattcgca tcgattactt gtctcttcgt ccgaatatca
     1261 gcgctgcttg ctttaaaata tcattttcca tttctaattg ctggtttctt ttacgtagtt
     1321 ctaacaattc tttttgttca ggcgtaagat tatctttttc tttgaatgaa ccactcgttt
     1381 tagattgctt tacccatttg tcaaatgctg aagccgttag ttcatattct cgaatgattt
     1441 ctacacgtgg ctttccagct aagtaaagat tgacgatttg ttgcttgaat tcttgtgaaa
     1501 aagttcttcg tgttctctta gacataaaaa ttcctcctgg tatgttttct tctagtctac
     1561 acaccttaat ttttctgtct agttaattgt agcctatcca agtctacact ctgagatgta
     1621 agaaatatca taagatattt cttacatctt tttgttttga gcaggacttt agataatttt
     1681 attaatatat gctagcatgc ttttgtaaga aaaagaaaga gggcgtatta gttatgtatc
     1741 ttaaaattac ggaacaagca caagaaagat tacaaaaata tattgatgaa ggcgcaacag
     1801 ttatattgga tttagatgat ggcgttgggg aatattctaa aatgggtgtt tgctctttag
     1861 atactagttt tcgtttactt cttcttgata aagaacagag aaaaaatgat tataaactcc
     1921 aattagatag tgatattggg gatgtttata taaaagatta ttcaaaaatg tacatggatg
     1981 aacatatgac cctttcttta gatccacgtc taggtgtatt tactcttgaa agcccaagtg
     2041 gttctttaga cagtcatgtt caattagtag atctacgacc agaaaaattc tctggaataa
     2101 aacaataact ttctctctat tgtccataaa actagtctca atcaagctgt attgaaaaaa
     2161 caagacaaca ttcttaccat ggtaaataaa aagacgacac attttgtcgt ctttttttaa
     2221 attaactatt ttcgataaaa gatcgcatag cttgtgccat tatttctggc ttttctctat
     2281 tcaattcatg ttttccgttt ttaataaaga gtagccggcc attcgttgaa agtttatata
     2341 aacgttttgc tgctggttta ttcgcatgat ctttggtacc gcataaaatc aatttaggaa
     2401 gtcgtgatgt acgaagctgt tttttgcagt ctaatgtttc aagtgaacga agtaggggaa
     2461 gggtttgttt tttagataga cctaattttt caaatatctt agaaggtacg cagtaaaaat
     2521 aaatattctg gaaagtaagc aataagcgat taggagactc aaattgtgga gcgactaaaa
     2581 caaccttttt taaatatgga gaaggagttg caagatagtt taaagcaata acagcgccta
     2641 aagataatcc acataaaata aaaggttcct ttatctgatt caattgtact tcgatgtatt
     2701 tggttaagtc gtctaaagta gcagtgtctg tgctgtttaa atgattgaac aatgaaagac
     2761 ttttgcaaga ataactattg ggtagataag agattacatc tttccatgat tgttcgtctt
     2821 ggcctagacc atgcaaaaaa acaattttca tattgctctc ctttttatag ttatcatata
     2881 gactttcttt gtactattaa aatataaaca ttcatttctt acgtcataaa ataaaaaaac
     2941 agcgcagaca cattgttaaa ccaacatgtt gcactgttta aggaagccac ttgccgggct
     3001 tgaaccggcg acctcttcct taccatggaa gcgctctacc agctgagcta aagcggcatt
     3061 atcattaata caaataatag gataacgaat attaatgtat ttgtcaacag aattttttcg
     3121 aaaaaatgtg tttctctaat aggaaaacag attttagaaa aaataaaaat aacaaaaaat
     3181 gcgaacggtt tctttgataa tataataaaa aaattcaaaa aaagtattgc gttatttaga
     3241 agaaattgat attatattca tgtacttgat tccggcatag ctcagttggt agtagcgcat
     3301 gactgttaat catgatgtcg taggttcgag tcctactgcc ggagtaaata aagcttatgc
     3361 aaagaaaaag aaatccaatc acttgggttt ctttttcttt tattattatg atgaatcaag
     3421 ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag agtagagttg gctactcata
     3481 atcaaccata cttcccatca tcgctaaata cgaaataaat cagcattcct acaccaatca
     3541 aagcacaagc tgcaccaaga aaagggatga cattcccaaa gttatctgta ttcataagta
     3601 tgccaccacc cacaaacaaa aagccgccag caatcaggtt tttcatttta ttcatctcct
     3661 ttcttacgtt atagtatttc cttctttcaa tgagtatttt gaaagctatc ttattgtatg
     3721 atgttcttct aggcctcttc tttattatac aggccatttt cgttatctca tagaaatatt
     3781 cctttttatc tgaaaactat tgtataccgt atgacatttt tcttcatgaa gttgtaagta
     3841 tattctcatt caaatgaaaa tttcatcata gaagagattg tatttctaaa ttcttaagag
     3901 taaaatacga agtataactt ggatttggaa ggatgattgg atgaaagcta ttacgcctga
     3961 tatgacaaca aaatttaaca aacagtttca agcaaatatc aaacagcagg cattgcagcg
     4021 ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat gtttcagcac atgtggagaa
     4081 tacaccggta ttttctatag atttggctac ggggaaagtg gctaatcaaa tgcaatcggg
     4141 gcgttgctgg atgtttgctg cactgaacac attccgtcat aagatgctga atcaattcca
     4201 tttaaaagag tttgaattat cacaaaatta cacttttttc tgggataaat atgaaaaggc
     4261 aaattatttc tatgaaaaca tcattgcaac aggaaaagag gcaatcgata gccggaaagt
     4321 agctttctta ctcgctactc ctcaacaaga tggcggccaa tgggatatga tcgtttcatt
     4381 attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa
     4441 ttctcgagat ttgaataact acttgaacaa attactgcgt aaagatgcag tagtgttacg
     4501 gaaaatggtt gctcaaggag aaacattaga cgctattgaa gaaaagaaag aagaaatgct
     4561 agaaagcatt tataacttct tagctatctc attaggaaca cctccaaaag aattcgattt
     4621 tgagtaccgc gatgaagaaa agaactatca tcttgaccga ggactgacac cacaaatttt
     4681 ttatgataaa tacatcggtg taaaattaga tgattatgtt agcgtgatca atgcaccaac
     4741 aaaagacaaa ccattcaatc gttcttatac agtagagatg ctaggaaatg tcgttggcgg
     4801 gaaagaagtc aaatatctga atgttgatat ggaaactttc aaaaaattag cgattgctca
     4861 gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta ggtcaatctt ctactagaac
     4921 tagtggcatc atggccttag atgcttattc aatggatgat ctatttgata cggattttac
     4981 aatgactaaa gctgaacgtt tagattttgg agaaagtctg atgacccatg cgatggtatt
     5041 gaccggtgtt gacttagtag acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg
     5101 cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat gcctggatgg acgaatatac
     5161 gtaccaaatc gttgtaagaa aagatttatt gacgaaagag caattaaatg cgttcaatga
     5221 agaaccaatt gttctatctc cgtgggatcc aatgggtgct ttagcataga cgagtatttc
     5281 aatgaagagt atgtagtaaa gataaaaaga gctggcttaa gccagctctt tttatcttta
     5341 aagtccattc cctgtttttt atggtccctg tgctataatt ctaaataaag gataaggatt
     5401 ggaaataatc cttttatcat taaaaatatg atgacgaagt agaataaaat tgggagtagg
     5461 ggggaaagtc atggatatcc aaactgtttt atctgatctg aaattaaatg gtaaagtgat
     5521 tccagttgtt ggtggcgatg ttaatcagac ctatcgtata aaaaccgaac atcgtgctta
     5581 ttttttaaaa atccatccga atgtaaaaaa aggatttttt gaagcagaag tagacggatt
     5641 aaaagaactg tcagcattcg tacgtgtacc ggatacgtat atgctaggtg aaacaagtga
     5701 aggcgcttat ttgttaatgg aatggatcga gccgggaaaa ggggatcaac gagatcttgc
     5761 tgcagcttta gctaatctcc atcagcaaac agcgccacaa tttggttttc gtaaagataa
     5821 ctatttgggt acacttgttc agaaaaattc ttttgaagaa gattggtgga cttttttctt
     5881 taaagatcgg cttgaatccc agatttcttt ggcagaagaa acgaatcgat ggaatgtaca
     5941 gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa
     6001 gatcactcca cgcctcttgc acggagacct gtggagtggt aatgtgttct ttgaccagca
     6061 agggcatcca gtttttgtcg acccagctgt ttcttatggg aatcgggaac aagatattgc
     6121 aatgagccag ttattcggtg gtttccgacc ggaattttta gatgcttatc aaactatatt
     6181 tccattagaa aagggatgga aagaccgact gccgatttac cagttatatt atcttttggc
     6241 acatttgaat atgtttggag aaagttacgg ttcccaagta gatcaattac tagaaaattt
     6301 ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa aagcatgaag tttcatatta
     6361 tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg ttaggagtag ttatcaaaac
     6421 gtctgaagat caaagcgacc agttgggaag aggcagtgaa ttcgtcaata gctttggact
     6481 tacttgggtc atcacgaatt attccatgga gattaatcgt ctccctagag tcggtgaagt
     6541 aatctcagta acgactcaag ccatggagta caataagttt ttctgttatc ggaacttctg
     6601 gatccatgat gaagatggga aagaattagt caaaatagaa tctgttttcg tattgatgga
     6661 ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc attgctcctt ttgagagtga
     6721 gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt
     6781 gccatatcgt gttcgttttt atgatatcga tagtaatcag catgttaata atgcgatgta
     6841 ttttaattgg atcattgacg tattaggata tgactttttg actacgcatg ttccagaaaa
     6901 agtaattatt cgatttgata aagaagtaga gtacgggaat gagatcgaaa gccattttga
     6961 acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct
     7021 gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa agaggttgtg acagaagggg
     7081 gcaacttcaa gaaataagaa gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt
     7141 cgacttagtt ttcgaagaag ctatttttgt cacagcctct ctttttaagt ttagctatta
     7201 gataatgatg tttagttttg gataaacgaa atcaattctt cacccgacaa accatagttt
     7261 acagcaatca atagttttaa acgtgctttt tgactattaa tactgttaca gaaaataatc
     7321 cccatttttt ctagttcttt tcccccgcct tcataatcat aaacaggctc tgcgattcca
     7381 ttgaaacaac gagaaacaag tactacaggg attttttgga caagaagctt ttccaaagca
     7441 gctaaggctt gtggagggag attccccgca cctaaagctt cgattaccaa accgtctact
     7501 ttcgtatggg cgatggcttc caataagtcc ccttgcatcc cagcatatgc tttaacgatc
     7561 gggatagtcc catctacagc agaaatgtcc agtcgctttg tttctaaaag ttcctgcaag
     7621 aataaaattc gattcttagt tactaagccg atgggaccca atgtaggtgt tcggaaagta
     7681 gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag aatggatctc atcgttcatg
     7741 accaccaaaa cccccttatc tagcgcttct tcacaacttg cgacacgaat cgcactttca
     7801 aaattataaa ggccgtcact tccaagttca ttacttgaac gcattgcccc agtcaaaaca
     7861 attggtaatt tgttgccaat cgttgtatca agaaaaaaag cagtttcttc taatgtatct
     7921 gtaccgtgtg tgatcaccac tccggatata cctgatgcat aagcggacaa gatacgttct
     7981 tttaatacca acatgtgaga aggcatgatg tgtggtgagg gaagattgaa gatatcttca
     8041 actaataatt ctacatgctt ggggattgtg atttctgcat ctaataatgg gtttgccaca
     8101 tcaggactaa caccgcccgt gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt
     8161 agtacaagta tttttttcat tttacagact tccttacgaa taattttgca tagccgttaa
     8221 atcttattgt atactatata aaagagtttt gctatcagtg aagaataacg aatagcctaa
     8281 cttatcataa aaaagctgac cttattaaaa tggaaaagac tcaaccttga cctcctaata
     8341 caatggagta tctggttgag tttcttttac aagaaagcag ccaaggaaat caatcacgaa
     8401 ggtggccgga aatgatcaga atcggattaa catcgtttaa agaacatgac aaattaactg
     8461 gtaaaaagaa taacactctt tatgaatatg cagggtttct acccttagtg gaattagata
     8521 ctgcctatta tggaattccc cgaaagtcct cagttgagaa ttggataagt gaagtacctg
     8581 aatcatttcg ctttgtagtc aaagtctatc aaggaatcag cggtcaagga gactggaagc
     8641 cttattatca gaccgaagaa gagatgattg cgcatttttt agatgttatg tcgccaatgg
     8701 tagatgctgg aaagatgttt tgctttttag tccaattccc ctcatcattc aaatgtacga
     8761 aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt tgagggttat cctgtttcta
     8821 tagaattgcg ggattattct tggtacagcg atgaatttct tgaacgtaca agaaacttta
     8881 tgtctagagc cgctttttct ttagccatca tcgatgagcc acagttgctg aatacaacga
     8941 ttccttttga tccatttgtc accaatccga actttgctct ttttcgattc catggtagga
     9001 atgcgcaagg atggcaagca aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt
     9061 attcagaaaa agaactgcaa gaactagcac atgaagtcaa agtagttgat tctaagacaa
     9121 aagaggtagg tgtgattttt aataataatt ctggtggtga tgcggctgaa aatgcattag
     9181 cactgcaaaa aatattaaat atcaagccag atggactcaa tccaacccaa atggatctgt
     9241 tttaagggct tattttattc aagaaagggg cgaagtctat gatcaaagct attttttttg
     9301 atattgacgg aacattggtc accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa
     9361 ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc aacggggcgc agcccagtaa
     9421 agatcaaaga aatcattgat gaattggaat tagatatgta tgtcgtgtat aatggtcaac
     9481 tggtttttac tgcagatcgc acgattatcg accacccttt tgaacaaaaa gtattggaac
     9541 atattgttga atttgcagat gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt
     9601 tagatggatc aacgacgatg ctattaggac aatcgatttt tatcaagcga ctagtcagct
     9661 ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat cctccaggtt tttagccctc
     9721 accgacaaaa ggatcgttat gaaacgttag agatattaaa agaaccaatt tatcaatgta
     9781 tattgctcag tccggagtca gaacagaaaa agttagaaca tcgctttccc gagtgtacgt
     9841 ttcagcgttc aaatacgtat actgtagata tcatcccaaa aggtggttca aaattactag
     9901 gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg
     9961 atcattacaa tgatatcgag atgctgaaag gtgtaggaat tggagtggcc atggggaatg
    10021 ctcagatcga agtaaaacat gctgcagact ttgtgactca atcaaatgaa gaagatggga
    10081 tatacgaagg actgaaacac ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc
    10141 agtatggaag cagagacacc ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca
    10201 agaagaccag agatacccac ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc
    10261 aaaatagaag aacttgttga atttgttgca gctacttcac ctaataaaga acaactgcat
    10321 gaagctgtta tgctgctgca cgaagcatta gataaagcag aagaaaaaat acttggtaaa
    10381 acacagtggg gaagtgatcc gctggttgaa caagtagatg cactgacaga tatcctttac
    10441 tttacttatg gttctttttc attactagga atagtgcctg atcgaatatt cgaaatcgtc
    10501 catcaggcaa atatggggaa attatttcca gatggaaaac ctcattatga ccctgtaaca
    10561 aataaagtgt tgaaacctga aaactggcag atggaatatg ctccagaacc taaaatcgcg
    10621 gcagaaatcc aacgtcagat tcaagaagcg attgctaaga agaaatccgc tgatataggc
    10681 atgtgcacaa atgaaaaaga gtgatcagag tttcactatt tttgttatct ttatacgatg
    10741 gaaaaacaaa agattttcgt ttaagttagt gaatgcatga aaatagtatg ctataatcta
    10801 gaatagatgt cttttgcacg agagacgtat tatttggatt tatgccaagc agaacgccat
    10861 ctgttgcata aaagatgcat actgaaaacg gaggaaccat aaatgggaaa taatgttatc
    10921 atcggaatcg tcattgcaat cattatagtc gccattgtcg tctttttggt tagttacttg
    10981 atgaagaaaa aaaaccaaga tcgattggat gccttagaaa aacgcaagga agaattattt
    11041 gacttgccag tgattgaaga agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc
    11101 caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct
    11161 gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa
    11221 ggaaaaaaag cagttgttga agcagaagct acaatggacc aaatggagaa acaagtgacg
    11281 gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag aacgcaactc actagctgtt
    11341 cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa
    11401 tacggaccag ctctttttga attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg
    11461 cagtttgtga ctttaaacac ttctggagac cctgttgaag cccgcgaggt tttggataaa
    11521 gcagaaaaac atacgtatga agtggaagct ctgatgaaaa aaattccacc gctgtatgag
    11581 gagctccata ccactttccc tgaacaactt gaagaaatca gtgatactta cgagaaattg
    11641 atggaagaac aatatgtatt cccagaagaa gatcttgctg aagatatcgc aaaagtaagt
    11701 cgaagaattg aaaattcatt agccaatctt gaaaaaacag aagttgaaac ggtcgaattt
    11761 gaaaaccgtg aaacagctga tttgattgat tcgttatatg atatcttaga acgggaaatg
    11821 gaagctcaga gatatgtaaa aactaaccaa tcaacgattg cagagtatat caaacataca
    11881 acaaagaata accgtcaatt attgattgag ctggatcaca catctcaaag ctatacattg
    11941 aaccacaatg aaattggccg tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt
    12001 caaaatgaac agatgattcc acagatccgt caacacgaaa ttccttattc agaagtgcgc
    12061 acgttctata aaacagtctt caaagtctta gaagatatcg aaaagcaaca agtagagatc
    12121 gacgactcat tacatgaatt gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca
    12181 ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg
    12241 ccgaatgatt acctagaatt cttttttgtt gctactgacc gtattgaaga actaagtgtt
    12301 gtcttgaata aaattcgagt aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa
    12361 gaagatctag aattattaga taagaagaca catgatttag tagatgcagc agcattaacg
    12421 gagcaaatgc tccaatatgc aaatcgttat cgtcatacac acgaagaagt gagagaagca
    12481 attgataaaa gctactattt attcaataaa gaatatcact atcaagaagc attagacgaa
    12541 atcggtacag cattagagcg agtagaacct ggtgctttca aacgcatcga agatttttat
    12601 tttaatcatc cagacttagt ataaagcaaa aaagttagta aaccagcaag agcaagacgg
    12661 attattgtct tgttcttgtc tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc
    12721 acctgctctt gaaagcacct tggacaaccg ttataataga aaagaatttc aaaaaagagg
    12781 ggtttcttat gatatatttt gataacagtg caacaacccc aatctttccg caaagcttag
    12841 atgcatatgt aaaaaccagt cagcggatta ttgggaatcc atcaagcttg catgatttag
    12901 gcaatcaagc taaccggttg ttgcagcaag caagaaagca gatcgctgat ttgatccatg
    12961 tagaaccaga agaaatctat tttacttcag gcggaacaga aggagataac tgggtattaa
    13021 aaggaacgat gatcgaaaaa agagaatatg ggaatcatat gatcatttca ggagtcgaac
    13081 atccggccgt cagcgagaca gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg
    13141 ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag
    13201 agacagtatt agtctctgtt atggctgtga ataatgaagt aggatcgatt cagcccatcc
    13261 aggaaatcag tgatcttttg gatgattttc ctaaaattca tttccatgta gatgctgttc
    13321 aggcgattgg aaaagtacca ttctcgcaat ggttgacaga aagagttgat tttgcaacat
    13381 tttcagctca taaattccat ggcccacggg gaactggttt catttattgg aaaaagggtc
    13441 gtcgtttagc accattactg acaggtggag ggcaagaaag aaatcagcga agtgggacag
    13501 aaaatgttcc aggtattgtg gctatggtta aagctttacg tttatcatta gagataaagg
    13561 aacaaaaacc tgagcagaca caacgcttaa aagtagctct aatcaatgcg ttgaagacgt
    13621 atgagaaagt gacgattttt tcagaaggaa ctgactatgc accgcatatt ttatgttttg
    13681 ctctaaaagg aattcgcggc gaagttctcg tccatgcctt agaagaaaaa caaatatttg
    13741 tttctactac tagtgcctgc tcctcacgta aacatatggc aagcagtaca ttgcacgcaa
    13801 tgcatgtacc aaatgatctg gctacatctg ctgttcgaat cagcttagat gaaaacaatt
    13861 cgcttgcaga agtcgagcaa tttatgatta ttttcaagca gctttataag aaattttctg
    13921 caatcaatta aaagaggagt gttcttgtga aatacagtga aattatggtc cgttatgggg
    13981 aactttcgac aaaaggaaag aatcgaaaaa gctttatcat gcaactagca caaaatgtca
    14041 gacaagcttt gaaagacttt ccagaaatca aaatccatgc tgatcgggac cggatgcatc
    14101 ttttattgaa tggcgcagac gggcagctag ttattccaaa actagcaaaa atatttggga
    14161 tccaaaattt ctctccaagt attcgtgtag aaaaagatgt cgaagttttg aaacaagctg
    14221 ttcaagacat catgaaagaa atttatactg ggaaagaaac atttaaaatt atcgctaaaa
    14281 gaagcgatca ccaatttgaa ttgacaagta atgaattgaa tcaaacatta ggtaatgctg
    14341 tttttgatat tttcccgcat atccaagttc aaatgcgtca accagatatt ccattacgtg
    14401 tagagattcg gcgtgatggc gcttatctat cgtatgaaac gatcaaggga gctggaggat
    14461 tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc tggggggatt gattctcctg
    14521 tagcagggta ctttgccatg aagcggggag tggaaattga agctgttcat tttgctagtc
    14581 cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga tctaacagcc aaactagcac
    14641 cttatgtggg aacgatccat tttatcgaag tgccatttac cgagattcaa gaagaaatca
    14701 aaaagtcagt gccacaagga tattggatga caattactcg tcgaatgatg ttgcgtttga
    14761 ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc
    14821 aagtggcttc acaaactttg caaagtatgg tggctatcaa tgaagtaacg aatacgccaa
    14881 tcattcggcc tgtagcgaca atggataaac ttgaaatcat tgaagtagca gaacagatcg
    14941 atacatttaa tctttctatc cagccttttg aagattgctg tacgattttt gctcctccac
    15001 agccaaaaac acgccctcaa ctggaaaagg tccatcaata tgaagagcgt ttagcaatag
    15061 atgaaatgat ccaacgagca ctagctggct tgaagatgga agagatacaa gcagaatcgt
    15121 ctaatgaaaa aactgaagaa ttttcagaat tgctataaga ggttatgaaa atagtgtttt
    15181 gaccagagta ttagagaaac aaaacgaaaa aatagctcca tgtattttac cgtttttgaa
    15241 ctaatgtcga agattagctg attgaatacc atttatcaag aagttatgga aaagccggtt
    15301 cttttttact aactgaaaaa taaaatttta tttagagatc aagctacagt tgataaatga
    15361 tataaaaaag agaatttccc atgaaatagc gaggttgtga cagaagtggg cagcttcaag
    15421 aaataagaag gaatttacga aaattgcttt tcaaattttt gtgaaatttc ggcttatttt
    15481 tcgaagaagt tacttctgga acaccgtgta tcagaggttg
//
LOCUS       DAFFQY010000066        15346 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFFQY010000066 DAFFQY010000000
VERSION     DAFFQY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.066"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..563)
                     /locus_tag="LRB02_001941"
     CDS             complement(<1..563)
                     /locus_tag="LRB02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL4660132.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LRB02_001942"
     CDS             complement(750..1400)
                     /locus_tag="LRB02_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660133.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LRB02_001943"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LRB02_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL4660134.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LRB02_001944"
     CDS             complement(2912..3910)
                     /locus_tag="LRB02_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL4660135.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LRB02_001945"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LRB02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL4660136.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LRB02_001946"
     CDS             complement(4926..5657)
                     /locus_tag="LRB02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL4660137.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LRB02_001947"
     CDS             complement(5661..6926)
                     /locus_tag="LRB02_001947"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL4660138.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="LRB02_001948"
     CDS             complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="LRB02_001948"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL4660139.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRAFGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="LRB02_001949"
     CDS             complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="LRB02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL4660140.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12267..13769
                     /locus_tag="LRB02_001950"
     CDS             12267..13769
                     /locus_tag="LRB02_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL4660141.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13771..14508
                     /locus_tag="LRB02_001951"
     CDS             13771..14508
                     /locus_tag="LRB02_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4660142.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14553..14726)
                     /locus_tag="LRB02_001952"
     CDS             complement(14553..14726)
                     /locus_tag="LRB02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660143.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14702..>15346
                     /locus_tag="LRB02_001953"
     CDS             14702..>15346
                     /locus_tag="LRB02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660144.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4520 a         3288 c         2589 g         4949 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg aattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt tttaactatc tcagctatct atcctcttta taccattatt
     7201 tttttcaaat agtgacctgt ataactttct tttacgttaa tgatttcttc tggtgttcca
     7261 gtcgcaagga tcgttcctcc accttctcca ccttctggtc ctaaatcaat gatataatct
     7321 gctgttttga ttacatctag attgtgttca atcactaaaa ctgtgttacc agcatctact
     7381 aatctttgta atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc tgtcgttggt
     7441 tcatctagta tatagaaatt ctttccatta gagattttgt gcaattcact ggcaagtttc
     7501 atccgttgtg cctcaccacc ggacaatgtc gttgctggtt gccccatcgt cacataacct
     7561 aagccaacat caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg cttgaagaat
     7621 tctacagcat cctctactgt catttccaaa atatcagcaa tgctttttcc tttgtaatgc
     7681 acttctaatg tttcagaatt atatcgttta ccatgacata cttcacaagg aacatagaca
     7741 tcaggcaaaa agtgcatttc gatcttaata attccatccc cgcgacaagc ttcacaacga
     7801 ccgcctttta cgttgaaact aaaacgccct ttcttataac cccgcatttt tgcctcgttc
     7861 gtttgagcaa ataacccacg gatatcatca aatacacttg tataagtcgc tggattactt
     7921 ctcggcgtcc ggccgattgg gctttgatcg atatcgatga tcttttcgat actttcgtag
     7981 ccggaaattg tcttgaattt acctggctta gcagaattct tatttaattt ttgcgctaac
     8041 gatttcttta agatactatt gaccaatgta ctctttccag aaccagacac acctgtgact
     8101 gctacaaatt ctcctaatgg aaattcaaca ctaatatttt tcagattgtt ttctgaagca
     8161 ccagtgatct tgattgcttt cccattacct ttacggcgtt cttttggtac agggattgat
     8221 tttttccctg aaagatattt ccctgtcaaa gaattttcat cattagctac ttcttcagga
     8281 gtgcctgcag ccacgatttg tccgccttgt tctccggccc caggtccgac gtcaatcaaa
     8341 taatctgctg cccacatcgt atcttcatca tgttcgacta caacgagggt gttgccaaga
     8401 tcacgcattt tctttaatga gccaatcagt cgatcgttgt cgcgttgatg caatccgatc
     8461 gatggttcat ctaaaatata caaaactcca gaaagattcg atccaatttg tgtagccaat
     8521 ctgattcgct gcgcttctcc cccagataat gttccgaaag cgcgactcaa agttagatag
     8581 tctagtccca cattttccaa gaatgaaagt ctgtcattta cttcttttaa aatcggtttg
     8641 gcaattactt tctcttgttc agataacgtt aagtttgaga agaaatctac tgctttttga
     8701 atagacaact catttgtttg cccgatattc gttccattga tttgaacaga tagagcttgg
     8761 ggattcaatc gataaccttt acatgtctga caagttaatt ctgtcatata aagacgcatt
     8821 tgctcacgcg tgaaatcact gttcgtatca tgataacgac gctcgatatt cttcaatacc
     8881 ccttcgaaag tcgtttctac atcgcgaacg ccaccaaaat cattttcata atggaaatgg
     8941 aaaagatcat ctgtcccatt caagatgatc tcctgatgct cttccggtaa ttcttcgaat
     9001 ggtgtatcta aatcgatacc gaaactttca caagcctgcg ctaacatttg aggataatac
     9061 tgagagctga ttggattcca tggaacgata gcaccttgac taagtgtctt cgttctatct
     9121 ggaatcacta aatcttgatc gacttctaat ttgacaccta aaccatcaca gtctggacaa
     9181 gctccaaaag gcgcattgaa tgagaacagt cttggttcta gttcgccaac agtaaatccg
     9241 caataaggac aggcatagtg ttcgctaaac aacatttctt cttcaccgat cacgtcaacc
     9301 agagcatagc cgtctgctaa tcgcagggcc gcctctaaag aatcaaatag gcgagaacga
     9361 atcccttctt tcacaatgat ccggtcgatg acgatcgcga tatcatgttt tttgtttttc
     9421 tccagctcag gcacttcact taaatcatag atctcaccat cgactcgaac gcggacgtat
     9481 ccctcttttt ggattcgttc gaatattttt ttatgttgtc cttttttctt taccacgatc
     9541 ggtgccaaca cttggatctt tgtgcgctca ggcaaagcca ttacttgatc tgccatttga
     9601 tctactgatt gactggtaat ctcgatatga tcgttgggac aaatcggatg tcctacacgt
     9661 gcaaacaaca agcgtaaata gtcattgatt tccgtcacag tacctactgt cgaccgagga
     9721 tttttacttg ttgttttctg atcaatagaa atcgccggac tcaatccgtc gatgctatct
     9781 acgtccggct tatccatttg tcctaaaaac tgacgagcat aagcagaaag gctctctacg
     9841 tatctccgct gcccttcagc gtacagcgta tcaaatgcta gggaactttt tccagaaccg
     9901 gacaaacctg taacgaccac gaatttatct cgtggaatcg ttacatctat atttttcaag
     9961 ttatgggcac gtgcaccatg aatcgtaatt ttatcgttag ccatgttgtc ctcctacttt
    10021 gaagctttta attctaaaat catatctcgc acattcgcgg ctgtttcaaa atccaaagct
    10081 tttgccgcat ctttcatttc tttttctaat ttcatcaata acgtgtcttt ttcttccttc
    10141 gtcatttctt cgtacgagac ttgtaccgct tcttttgtat catcttccga ttctttcgta
    10201 atagaaatca aatctcggat ttctttaatg atcgtttttg gaacgatccc atgttcttca
    10261 ttgtacttct gctgaatcgt acgacgtctt gacgtttcat ccatcgctag acgcatagaa
    10321 tctgttactt tgtctgcgta catgattact ttaccttctt cattccgagc agctcgtcca
    10381 atcgtttgga ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc atccaagata
    10441 gcaacaaggg aaacttcagg tacgtctaat ccttcacgaa gcaaattgat tccaactaac
    10501 acatcaaatt cacctaatcg aagatctcga atgatttctg ttcgttccag cgttttgata
    10561 tcactatgca aatatttgac tttgatccct aattctttga aataatctgt gaggtcttct
    10621 gccatttttt tcgtcaatgt cgttacaaac acccgctggt ctttttcgac tcgttcattt
    10681 atttcgccta ctagatcatc gatttgtccc atgattggac gaacttcgat cactgggtca
    10741 agcagccctg tcggacggat gatttgttgg ataacggtat ctgtttgttc atgttcatat
    10801 ggtcctggcg tcgcagagac ataaatgatc tgattgacat gtttttcaaa ctcttctaac
    10861 cgaagtggcc gattgtctaa agcagatggc aaacggaaac cgtaatctac tagcatctgt
    10921 tttctcgctc ggtcaccatt atacattcct ctgatttgag gcatggttac gtgcgattcg
    10981 tccgccacga tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg cggttcacct
    11041 tctttacgtc cgtccatatg ccgagaataa ttttcaattc ccgacgtgta gcccatttct
    11101 aacatcattt caatatcgta attggttcgc tgttctaaac gttgagcttc cagaagtttg
    11161 ttttcatttc gcaagacagt taaacgctgt tctaattccg ctttgatatt cgctacggca
    11221 tgctccatat gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc tacgtgttcc
    11281 gtttctccta aaacttcacc tgtcagtgca ttgacttctc ggatacgttc gacctcatct
    11341 ccaaaaaact ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat ctccacgaca
    11401 tcaccgcgca ctcgaaaacg accgcgctga aaatctatat catttctttc gaattggata
    11461 ctgactaagt cacgaatcaa ctgattgcga tctagttctg ctccctgtcg aatcgaaact
    11521 acttgttttt ggtactcaaa aggcgagcct aatccaaaaa tacatgaaac ggatgcaatg
    11581 acgatcacat catttctttc taataatgaa ctagtagccg aatgtcgtaa tttgtcgatc
    11641 tcatcattta cacttgaatc tttttcgata tacgtatcgc ttgaaggcac ataagcttca
    11701 ggctgataat aatcataata agaaacaaaa tattcaacag cattgttagg gaaaaattcc
    11761 ttgaattccc cgtacagctg accagccaat gttttgttat gggcaataat cagtgttggt
    11821 ttattcactt tctcaataag attggaaatc gtatatgttt tccctgttcc agtcgcacct
    11881 aataagattt gtgctttttt cccgccgact acgccatcca ctaattggtt aatcgcttca
    11941 ggctgatcac cagccggttg gtatttcgag acaagctcaa agtgtcggga ggtatctctc
    12001 tcaatcattc ttcgtcctcc ttttcattca ttccttttca ttttacgacc aataaaaaga
    12061 aaaggaaagc aaaggcattc cttactaaaa aattatacca caccgaacat actttcgcca
    12121 cttttttatc ttattataca tgttatgttt tataacataa aatgttagaa actcgcattt
    12181 ttttcatttt ttcgagctta cagtcttgtc tgattcagca cttttcgcct ataataataa
    12241 aatattggaa atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt
    12301 tcttaatgac aggacttgtg acacttgcgt ttggtcatgt tgcacaagct gacaacacta
    12361 caacggcaga gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat
    12421 acgtaattgc cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt
    12481 acgaaggcat cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc
    12541 gaatggacta ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg
    12601 gaatgattgc cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt
    12661 cttactttga tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt
    12721 atgaagattt gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt
    12781 taagcgacca tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat
    12841 tgtacgacgg gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg
    12901 gttatgcgat caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa
    12961 aatatgcatt tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag
    13021 gattgaaaga attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg
    13081 atggttcaga aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta
    13141 tccaaaacaa ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga
    13201 tctcctttgc tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa
    13261 tacgcgctct tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga
    13321 tggttcttgc gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg
    13381 acttccttgc tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg
    13441 ttcgaggcgg gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc
    13501 tttcttataa tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc
    13561 catcattcgt caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg
    13621 gtgtagtcga attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt
    13681 atgtttactt gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag
    13741 caaaagtttt agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta
    13801 gtcaaaaaat acggcgataa cacagtatta aacgacatca atgtatccat caacgaagga
    13861 gatgtcgtct gtatcattgg cccttcaggt tcaggaaaaa gtacattctt gcgttgcttg
    13921 aatcgtttag aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat
    13981 aaaagcacag atatcaactt ggtgagacaa catatcggaa tggtattcca acactttaac
    14041 ctattccctc atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa
    14101 gaatctagag aagaagcaga gaaaaaagca atcaaattac tgcagacagt tggtctagaa
    14161 gataaaaaag atatgtatcc tgaaatgctt tctggtggac aaaaacagcg tgtggctatt
    14221 gctcgtgctc tagcgatgaa ccctgacatc atgctttttg atgagcctac ttctgcactc
    14281 gatcctgaaa tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg
    14341 acgatggtta tcgttacgca cgaaatgggg ttcgctaaag aagtggccaa tcgcgtcatg
    14401 ttcattgacg acggaaactt tttagaagat ggaacgcctc aacaaatctt tgaaaatccg
    14461 caaaatgaac ggacaaaaga tttcttgaat aaagtattaa atatttaata aaaaccaaaa
    14521 atgcacggtc gccatgaagg gaccgtgtat ttttatcttc ttattttctc acctaatcgt
    14581 atcagccgca tcgcttgttt tcctgcacaa acaagcaatc gctctacctg ttccatcgca
    14641 taagaaagta ccatcggttc attgatgata tcaagtacat agtcgatacc accctcactt
    14701 aattgagctt gatttttctt ctccatatta tgtagttgtg ggacaacttt ctgacaatca
    14761 gcaattgtgt caagaacgga tcaatccttt attggagttg ctcgaaaggg tcttctctta
    14821 ttacggacag gctttgctca ctgtacatag acaacaaatc attatcctag tcaatcggat
    14881 ttccagagcc tcattgctat cattgcttga taaaatccag acaaagttta ataaaatgta
    14941 tcagttacag ctaaactttg gaattgggtc gctttgttat acagagcaag agacaccgca
    15001 atctttcctt catgccaaac aagtttgcga atggatagct ttccatcaat ctgtgaacga
    15061 gatccgcttt tttgaagatt tagacctcgg gatcgtttta ccagcaatcc cgtcagatca
    15121 acgaacatta tatgtaaaac gtattttgaa aagcctgaca gaagaagaag tacatttgtt
    15181 caagaagact ttggcatgct tcagtaaaaa taacggaagc atcaaaaatt gctcagaaga
    15241 attatttatt cataaaaaca ccttgcaata tcgattgaac aagttccatt ctctcactgg
    15301 ttatacccct agaaactatg acgattatca tattctaaag ctggct
//
LOCUS       DAFFQY010000067        15240 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFFQY010000067 DAFFQY010000000
VERSION     DAFFQY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.067"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(58..360)
                     /locus_tag="LRB02_001954"
     CDS             complement(58..360)
                     /locus_tag="LRB02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660145.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="LRB02_001955"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="LRB02_001955"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="LRB02_001956"
     CDS             complement(1117..1890)
                     /locus_tag="LRB02_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4660146.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="LRB02_001957"
     CDS             2034..2384
                     /locus_tag="LRB02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL4660147.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="LRB02_001958"
     CDS             2365..3081
                     /locus_tag="LRB02_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4660148.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="LRB02_001959"
     CDS             3081..4529
                     /locus_tag="LRB02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4660149.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="LRB02_001960"
     CDS             complement(4599..5108)
                     /locus_tag="LRB02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660150.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="LRB02_001961"
     CDS             complement(5098..5823)
                     /locus_tag="LRB02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660151.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6046..8166)
                     /locus_tag="LRB02_001962"
     CDS             complement(6046..8166)
                     /locus_tag="LRB02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4660152.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8396..9574
                     /locus_tag="LRB02_001963"
     CDS             8396..9574
                     /locus_tag="LRB02_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL4660153.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9590..10348
                     /gene="aroD"
                     /locus_tag="LRB02_001964"
     CDS             9590..10348
                     /gene="aroD"
                     /locus_tag="LRB02_001964"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL4660154.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10371..10856
                     /gene="ybaK"
                     /locus_tag="LRB02_001965"
     CDS             10371..10856
                     /gene="ybaK"
                     /locus_tag="LRB02_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL4660155.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10927..12180)
                     /locus_tag="LRB02_001966"
     CDS             complement(10927..12180)
                     /locus_tag="LRB02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4660156.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12297..13646
                     /gene="gdhA"
                     /locus_tag="LRB02_001967"
     CDS             12297..13646
                     /gene="gdhA"
                     /locus_tag="LRB02_001967"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL4660157.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13758..15104
                     /locus_tag="LRB02_001968"
     CDS             13758..15104
                     /locus_tag="LRB02_001968"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL4660158.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5017 a         2712 c         3088 g         4423 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
     6061 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
     6121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
     6181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
     6241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
     6301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
     6361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
     6421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
     6481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
     6541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
     6601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
     6661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
     6721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
     6781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
     6841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
     6901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
     6961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     7021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     7081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     7141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     7201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     7261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     7321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     7381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     7441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     7501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     7561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     7621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     7681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     7741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     7801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     7861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     7921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     7981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     8041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     8101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     8161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     8221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     8281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     8341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     8401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     8461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     8521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     8581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     8641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     8701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     8761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     8821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     8881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     8941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     9001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     9061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     9121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     9181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     9241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     9301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     9361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     9421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     9481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     9541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     9601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     9661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     9721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     9781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     9841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     9901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     9961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
    10021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
    10081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
    10141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
    10201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
    10261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
    10321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
    10381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
    10441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
    10501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
    10561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
    10621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
    10681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
    10741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
    10801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
    10861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
    10921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
    10981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
    11041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
    11101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
    11161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
    11221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
    11281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
    11341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
    11401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
    11461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
    11521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
    11581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
    11641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
    11701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
    11761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
    11821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
    11881 atcctcttct tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
    11941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
    12001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
    12061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
    12121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
    12181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
    12241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    12841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    12901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    12961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    13861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    13921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    13981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    14821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    14881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    14941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
//
LOCUS       DAFFQY010000068        15136 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFFQY010000068 DAFFQY010000000
VERSION     DAFFQY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.068"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(81..1313)
                     /locus_tag="LRB02_001969"
     CDS             complement(81..1313)
                     /locus_tag="LRB02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL4660159.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSANPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1570..2139
                     /locus_tag="LRB02_001970"
     CDS             1570..2139
                     /locus_tag="LRB02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4660160.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2317..2757)
                     /locus_tag="LRB02_001971"
     CDS             complement(2317..2757)
                     /locus_tag="LRB02_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL4660161.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2915..3679
                     /gene="map"
                     /locus_tag="LRB02_001972"
     CDS             2915..3679
                     /gene="map"
                     /locus_tag="LRB02_001972"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL4660162.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3711..4634
                     /locus_tag="LRB02_001973"
     CDS             3711..4634
                     /locus_tag="LRB02_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL4660163.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4710..5849
                     /locus_tag="LRB02_001974"
     CDS             4710..5849
                     /locus_tag="LRB02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL4660164.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5842..6642
                     /locus_tag="LRB02_001975"
     CDS             5842..6642
                     /locus_tag="LRB02_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4660165.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6642..7469
                     /locus_tag="LRB02_001976"
     CDS             6642..7469
                     /locus_tag="LRB02_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4660166.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7447..8181
                     /locus_tag="LRB02_001977"
     CDS             7447..8181
                     /locus_tag="LRB02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4660167.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8281..9147
                     /gene="rfbA"
                     /locus_tag="LRB02_001978"
     CDS             8281..9147
                     /gene="rfbA"
                     /locus_tag="LRB02_001978"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL4660168.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9161..9733
                     /gene="rfbC"
                     /locus_tag="LRB02_001979"
     CDS             9161..9733
                     /gene="rfbC"
                     /locus_tag="LRB02_001979"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL4660169.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9755..10783
                     /gene="rfbB"
                     /locus_tag="LRB02_001980"
     CDS             9755..10783
                     /gene="rfbB"
                     /locus_tag="LRB02_001980"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL4660170.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10881..11732
                     /gene="rfbD"
                     /locus_tag="LRB02_001981"
     CDS             10881..11732
                     /gene="rfbD"
                     /locus_tag="LRB02_001981"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL4660171.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11766..13799
                     /locus_tag="LRB02_001982"
     CDS             11766..13799
                     /locus_tag="LRB02_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660172.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13843..15123
                     /locus_tag="LRB02_001983"
     CDS             13843..15123
                     /locus_tag="LRB02_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL4660173.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4614 a         2618 c         3164 g         4740 t
ORIGIN      
        1 gaaataaagg atctaagcca atagcttaga tcctttattc tatgctttat attatttttt
       61 gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag
      121 ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg
      181 gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct
      241 ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat
      301 aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg
      361 acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct
      421 ccttgttcgg cagaaaaatc aatgactttt ccatctttaa aagtaaattt catacccgag
      481 atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga
      541 ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga
      601 gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct
      661 gtaccgggag cagtatagtg cagggcagaa aattgttctc gattcaattc ttctgctttt
      721 tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga
      781 gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg
      841 aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt
      901 gcttgagttg ccttgcgtaa attcattagt gctttgccag ccgctgattg ataagacgct
      961 actcgatggc tatctacacc agcaaatgca tcgggattgg cagaaacaac actgatcctg
     1021 cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca
     1081 atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga
     1141 acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt
     1201 gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt
     1261 tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa
     1321 attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa
     1381 acaatgaggc ctttaaataa aaaaaagagg ttattttttt aaggttaagt ttcatgatat
     1441 atgttactac ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa
     1501 aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat
     1561 taaaaaaata tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat
     1621 gaagtagctg ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg
     1681 aattgttcca cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct
     1741 ctttttgagc agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg
     1801 aatacgatta tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg
     1861 tatcgttcac tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat
     1921 acttactttg tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag
     1981 atcgaatctt tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct
     2041 tcaagcatca tccatccaac tgatgaacag attgaaaaga tgatacaaga cagtatagat
     2101 gctatcttga aattcaaaga accaatagaa atcgattgaa tatactcaaa agttccacaa
     2161 atcagtagat aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat
     2221 gcatttttaa ctcccgaaga actcttccat gaatgcaatg aataaaagag ccgtacagat
     2281 tgaataaatc tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt
     2341 agcgaaagct tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt
     2401 ttcggctcct tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt
     2461 gcagaattca tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccgatag
     2521 atccaattct tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc
     2581 gtatgtataa gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg
     2641 ggtacattcg ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc
     2701 ggcgatttct tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat
     2761 gtgcctcctc gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag
     2821 aaaataaaac ctagagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta
     2881 aaatgagagg gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga
     2941 aattgaaatg atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga
     3001 ttttataaaa ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga
     3061 gagtcatggc ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat
     3121 cagtataaat gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga
     3181 tttggtaaaa gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc
     3241 ctatgtagtt ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc
     3301 tttgtacatc ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc
     3361 tatccaaact tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg
     3421 cgttggtcct acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg
     3481 gttgcggttg aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg
     3541 gaaaatgaaa atggatccta acggctggac tgcgtataca agagacggcg gactttcttg
     3601 ccaatatgaa catacattgg ctattactaa agatggtcca agaattttga catcacaagg
     3661 agaagaaggc acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt
     3721 ggaacaaaat aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca
     3781 tcactgattc agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt
     3841 tatttccatt gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga
     3901 ctgtactgac ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg
     3961 caatcaaaga ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg
     4021 ctttatggtc tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg
     4081 gcgtagaaaa tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct
     4141 tgttcatgat tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg
     4201 atgctattca gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga
     4261 agtggccaat cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc
     4321 ctaatgctca gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct
     4381 ggatgctgtt atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg
     4441 gttatcagat cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga
     4501 tcatcatttt gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa
     4561 tcaaaaaacg tcgagatcca agccgaaaat ggttcaataa attgaaaact aaattttctc
     4621 ataaaaaaaa gtagaaacaa gagttcttat tgtaaattta tatcagtaat ttataataat
     4681 tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg gttataagat
     4741 tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac
     4801 aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag
     4861 cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa
     4921 ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag
     4981 gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg
     5041 cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta
     5101 ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta
     5161 tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga
     5221 tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct acagtgtata
     5281 ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt
     5341 atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg
     5401 tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg
     5461 tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg
     5521 tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta
     5581 ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta
     5641 tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc atgttgtttg
     5701 ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa
     5761 aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga
     5821 agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt
     5881 gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa
     5941 gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa
     6001 agactaacag agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc
     6061 ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt
     6121 tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa
     6181 gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag
     6241 cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa
     6301 tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact
     6361 gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa
     6421 tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg
     6481 tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc
     6541 catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct
     6601 atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag
     6661 tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca
     6721 aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc
     6781 aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt
     6841 tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca
     6901 ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct
     6961 ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc
     7021 ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa
     7081 cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa
     7141 agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga
     7201 aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt
     7261 cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa
     7321 atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt
     7381 taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc
     7441 gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt
     7501 aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa
     7561 gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa
     7621 tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct
     7681 aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat
     7741 gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat
     7801 cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat
     7861 tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac
     7921 tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg
     7981 tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc
     8041 ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca
     8101 cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa
     8161 cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata
     8221 taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc
     8281 atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca
     8341 acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact
     8401 ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt
     8461 ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa
     8521 gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat tggagatgat
     8581 agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg
     8641 caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca
     8701 gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag
     8761 cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta
     8821 atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc
     8881 aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct
     8941 tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt
     9001 gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac
     9061 atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa
     9121 tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa
     9181 attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac
     9241 agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga
     9301 caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga
     9361 agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga
     9421 tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa
     9481 tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa
     9541 tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc
     9601 atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc
     9661 ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta
     9721 cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg
     9781 tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca
     9841 tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg ctggacttcc
     9901 tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct
     9961 agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt
    10021 aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc
    10081 ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct
    10141 tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga
    10201 aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt
    10261 aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta
    10321 tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata tcttaagcgg
    10381 tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga
    10441 tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat
    10501 cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg
    10561 tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact tgcgttatgc
    10621 aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta cgaacttccg
    10681 tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga
    10741 aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag aaaatgtcac
    10801 tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa
    10861 atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa ctaggtacag
    10921 aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat gcaaaagact
    10981 tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa cctgatgtta
    11041 ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag
    11101 atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa gctgtgaatg
    11161 ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat
    11221 acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg
    11281 aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg
    11341 ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac
    11401 tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta
    11461 tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata
    11521 gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac
    11581 ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc
    11641 tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca cttgcgcaaa
    11701 tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag aatttggagc
    11761 atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaaccagg
    11821 cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag
    11881 tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat
    11941 atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggagg
    12001 tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact
    12061 ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt
    12121 ctcctgcaaa ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg
    12181 gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg
    12241 gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta atcggtgcat
    12301 ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga
    12361 ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat catgataaaa
    12421 aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag
    12481 tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc
    12541 tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag
    12601 gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat
    12661 tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga
    12721 cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact
    12781 attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa
    12841 gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga
    12901 tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag
    12961 aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg
    13021 cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt
    13081 ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt
    13141 cgaaaataga taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag
    13201 tgattttagc atttgcatta tttggctgga gacgcttgtt ttacctcgct tttgccgcct
    13261 tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg
    13321 aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt
    13381 tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag
    13441 tacgctttta tccagatata gctgcatgga aggaaatcga tccgaaagaa gaatacgaga
    13501 agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca
    13561 ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag
    13621 ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc
    13681 agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc
    13741 cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat
    13801 aggctagctg agaataatcc tgaaatatta gtagaaagga gagtggagat aattcaagca
    13861 atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta
    13921 gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat atataacaat
    13981 agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca
    14041 aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac
    14101 tttcgggaat taagaaatga gtctttattt ggagatttac tgtttccttt gattatttta
    14161 ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt
    14221 gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca
    14281 gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat
    14341 acaatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt
    14401 gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt tctcgggatt
    14461 tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt
    14521 ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac ggttacgatt
    14581 cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc
    14641 ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt
    14701 ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg aaaccacaat
    14761 agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc tactttgctg
    14821 cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact
    14881 tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca
    14941 tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa
    15001 ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat
    15061 gatacatctt atggggaaca accagatagc gctcattctt ccaggatcca acgctatcat
    15121 tgataggcac aaaata
//
LOCUS       DAFFQY010000069        14994 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFFQY010000069 DAFFQY010000000
VERSION     DAFFQY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.069"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LRB02_001984"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="LRB02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL4660174.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="LRB02_001985"
     CDS             complement(321..890)
                     /locus_tag="LRB02_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL4660175.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="LRB02_001986"
     CDS             1076..1978
                     /locus_tag="LRB02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4660176.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LRB02_001987"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="LRB02_001987"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL4660177.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LRB02_001988"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="LRB02_001988"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL4660178.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LRB02_001989"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="LRB02_001989"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL4660179.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="LRB02_001990"
     CDS             8546..8731
                     /locus_tag="LRB02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL4660180.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="LRB02_001991"
     CDS             complement(8793..9989)
                     /locus_tag="LRB02_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4660181.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="LRB02_001992"
     CDS             complement(10159..11595)
                     /locus_tag="LRB02_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL4660182.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="LRB02_001993"
     CDS             11888..12697
                     /locus_tag="LRB02_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4660183.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..14880)
                     /locus_tag="LRB02_001994"
     CDS             complement(12883..14880)
                     /locus_tag="LRB02_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL4660184.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANSGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3177 c         2660 g         4780 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgctatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaa
//
LOCUS       DAFFQY010000070        14133 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFFQY010000070 DAFFQY010000000
VERSION     DAFFQY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.070"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            132..2603
                     /locus_tag="LRB02_001995"
     CDS             132..2603
                     /locus_tag="LRB02_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4660185.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2706..4442)
                     /locus_tag="LRB02_001996"
     CDS             complement(2706..4442)
                     /locus_tag="LRB02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL4660186.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4439..5143)
                     /locus_tag="LRB02_001997"
     CDS             complement(4439..5143)
                     /locus_tag="LRB02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4660187.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5274..5777
                     /locus_tag="LRB02_001998"
     CDS             5274..5777
                     /locus_tag="LRB02_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL4660188.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5737..6075
                     /locus_tag="LRB02_001999"
     CDS             5737..6075
                     /locus_tag="LRB02_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL4660189.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6096..7514
                     /gene="ffh"
                     /locus_tag="LRB02_002000"
     CDS             6096..7514
                     /gene="ffh"
                     /locus_tag="LRB02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL4660190.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7626..8204
                     /locus_tag="LRB02_002001"
     CDS             7626..8204
                     /locus_tag="LRB02_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL4660191.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8208..8729
                     /locus_tag="LRB02_002002"
     CDS             8208..8729
                     /locus_tag="LRB02_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4660192.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8808..9362)
                     /locus_tag="LRB02_002003"
     CDS             complement(8808..9362)
                     /locus_tag="LRB02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL4660193.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9615..9890
                     /gene="rpsP"
                     /locus_tag="LRB02_002004"
     CDS             9615..9890
                     /gene="rpsP"
                     /locus_tag="LRB02_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL4660194.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9902..10153
                     /locus_tag="LRB02_002005"
     CDS             9902..10153
                     /locus_tag="LRB02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL4660195.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10278..11069
                     /locus_tag="LRB02_002006"
     CDS             10278..11069
                     /locus_tag="LRB02_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660196.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11239..11760
                     /gene="rimM"
                     /locus_tag="LRB02_002007"
     CDS             11239..11760
                     /gene="rimM"
                     /locus_tag="LRB02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL4660197.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11750..12511
                     /gene="trmD"
                     /locus_tag="LRB02_002008"
     CDS             11750..12511
                     /gene="trmD"
                     /locus_tag="LRB02_002008"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL4660198.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12647..12994
                     /gene="rplS"
                     /locus_tag="LRB02_002009"
     CDS             12647..12994
                     /gene="rplS"
                     /locus_tag="LRB02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL4660199.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13252..13545
                     /locus_tag="LRB02_002010"
     CDS             13252..13545
                     /locus_tag="LRB02_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL4660200.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4725 a         2547 c         2892 g         3969 t
ORIGIN      
        1 ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
       61 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
      121 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
      181 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
      241 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
      301 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
      361 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
      421 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
      481 taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag
      541 atatagaaga ccttgtaata gatgacattg ttattttaga gactggatat caaattccag
      601 cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag
      661 cagatgaaat aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg
      721 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
      781 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
      841 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
      901 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
      961 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
     1021 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
     1081 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
     1141 aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
     1201 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
     1261 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
     1321 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
     1381 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
     1441 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
     1501 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
     1561 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
     1621 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
     1681 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
     1741 tcaagccaga acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta
     1801 tgacaggtga tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga
     1861 tggcttccgg aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact
     1921 tttcacgcat gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat
     1981 cagccagctt gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga
     2041 tctttgctct gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta
     2101 tcggacttcc atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat
     2161 tcatcatgaa tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg
     2221 gtgcgttggt gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat
     2281 ctaccatgct tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga
     2341 ataaattcag aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct
     2401 ttttgcataa gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca
     2461 ttctattttt ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt
     2521 ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat
     2581 cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc
     2641 actcgtgtat ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct
     2701 cttttttaca ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct
     2761 acagtaatcg taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg
     2821 gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt
     2881 tcctgatcct caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct
     2941 gttgcctgat agcggataat aatctcgctg tcgctttttg aatattggac agcattttca
     3001 atcagatttt tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt
     3061 ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt
     3121 tgctggaaat aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt
     3181 gacagctgga tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc
     3241 tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta
     3301 aaaccaatca gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc
     3361 ttttgtagct tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt
     3421 ccggtacctt ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct
     3481 gtaagggtga tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga
     3541 atgagttttg gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg
     3601 tttaaatgta gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc
     3661 aggaatacgc caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta
     3721 cacgcccgat acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct
     3781 tcccattgat cgggtgtttg catgattact tccgcctgat taggattttg caccattttt
     3841 tttagtacgg gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc
     3901 attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc
     3961 tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg
     4021 ccactttttt tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg
     4081 cgcaaagcgc tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt
     4141 cgctgttcat tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt
     4201 aaagtaatcc gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct
     4261 gcttggaggt tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag
     4321 ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca
     4381 aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc
     4441 atcgtttggg ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt
     4501 gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc
     4561 tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac
     4621 ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca
     4681 gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata
     4741 gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg
     4801 ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac
     4861 ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg
     4921 gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa
     4981 gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc
     5041 ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg
     5101 ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat
     5161 aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga
     5221 aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg
     5281 gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac
     5341 cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg
     5401 gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt
     5461 ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa
     5521 atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga
     5581 aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa
     5641 aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct
     5701 ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga
     5761 atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg
     5821 gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc
     5881 cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa
     5941 aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca
     6001 tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa
     6061 gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac
     6121 gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa
     6181 aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag
     6241 tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt
     6301 tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt
     6361 ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc
     6421 ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg
     6481 aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat
     6541 tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc
     6601 cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat
     6661 tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa
     6721 tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag
     6781 atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca
     6841 ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg
     6901 caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag
     6961 atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc
     7021 agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat
     7081 tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag
     7141 atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag
     7201 atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag
     7261 caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca
     7321 gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc
     7381 aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca
     7441 aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga
     7501 aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg
     7561 agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg
     7621 cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg
     7681 tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta
     7741 atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat
     7801 atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa
     7861 tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca
     7921 aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt
     7981 ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc
     8041 ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca
     8101 caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt
     8161 tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca
     8221 tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat
     8281 ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg
     8341 atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag
     8401 caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag
     8461 atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag
     8521 gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa
     8581 tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat
     8641 tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat
     8701 ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg
     8761 cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat
     8821 ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca
     8881 acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc
     8941 tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag
     9001 ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg
     9061 tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg
     9121 ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga
     9181 gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca
     9241 tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct
     9301 acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc
     9361 atacgatttt tcctccttca aactatctat aataatctta caaaagataa gtaactggag
     9421 caaataaaaa cacttactga caaaagctac atagaagata gattacttat ttttaacaaa
     9481 aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa
     9541 aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga
     9601 ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc
     9661 cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa
     9721 ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag
     9781 ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa
     9841 aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga
     9901 tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga
     9961 tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc
    10021 tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac
    10081 aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga
    10141 taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt
    10201 ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag
    10261 gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa
    10321 gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg
    10381 aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa
    10441 tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa
    10501 atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac
    10561 atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg
    10621 ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt
    10681 ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag
    10741 tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg
    10801 aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc
    10861 ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg
    10921 aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa
    10981 gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga
    11041 agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta
    11101 actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt
    11161 aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa
    11221 aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa
    11281 ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa
    11341 aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa
    11401 agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat
    11461 gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta
    11521 gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact
    11581 gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa
    11641 cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat
    11701 ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga
    11761 tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa
    11821 ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa
    11881 caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt
    11941 ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag
    12001 agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc
    12061 acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg
    12121 cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg
    12181 tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac
    12241 ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat atactcgccc
    12301 ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat
    12361 tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt
    12421 ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga
    12481 acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt
    12541 tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc
    12601 tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat
    12661 cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac
    12721 tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg
    12781 tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc
    12841 taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga
    12901 agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg
    12961 aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
    13021 ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt
    13081 ttctttaatt attggtaggg gagaataatg gattagaata aatctaccta taagtagtag
    13141 taaaaaaaag agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc
    13201 agaatattct aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat
    13261 acttatacgg agcaagaaaa gatgaaagtt ttagacacat tcatgaatga tggaaaagta
    13321 aaacaagtac caagttcaac aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc
    13381 tttgaccatg gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt
    13441 tctagtggcg attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga
    13501 agttcagacg gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata
    13561 ctcattgaaa aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt
    13621 tgtcatctgt atctataata caaagccaag aacgttattc aaaatatgga tagctatgat
    13681 aaaagctaat gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc
    13741 gtacatgaaa aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta
    13801 cgaataaata ccaaaacgta cctcctaaaa ttagattttg gacagatgcc ctttaaaaag
    13861 ataacaagat ttcgttcttg aaacaatata tatgctttta ttccaatgag gttgtgaaaa
    13921 cagtattttg tcctgagcgt tagaaaattg aaaatgggga atagcaactc gtactttacc
    13981 attttttaag tgaatacaag atcagctaat tgaacaccgt ttattcgtga tgagggacac
    14041 aacactgcag tcgacacatt tctcttttat gttcaaagat aacaaatatt taaaacaaag
    14101 aaaaatagta gataatcccg tcctaactct ttt
//
LOCUS       DAFFQY010000071        13836 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFFQY010000071 DAFFQY010000000
VERSION     DAFFQY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.071"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(24..1043)
                     /locus_tag="LRB02_002011"
     CDS             complement(24..1043)
                     /locus_tag="LRB02_002011"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL4660201.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="LRB02_002012"
     CDS             complement(1676..2680)
                     /locus_tag="LRB02_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4660202.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="LRB02_002013"
     CDS             complement(2693..3742)
                     /locus_tag="LRB02_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4660203.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="LRB02_002014"
     CDS             complement(3759..4580)
                     /locus_tag="LRB02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4660204.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="LRB02_002015"
     CDS             complement(4567..5346)
                     /locus_tag="LRB02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4660205.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="LRB02_002016"
     CDS             complement(5363..5833)
                     /locus_tag="LRB02_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4660206.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="LRB02_002017"
     CDS             complement(5845..6264)
                     /locus_tag="LRB02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL4660207.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="LRB02_002018"
     CDS             complement(6345..9122)
                     /locus_tag="LRB02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4660208.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="LRB02_002019"
     CDS             9313..9537
                     /locus_tag="LRB02_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660209.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="LRB02_002020"
     CDS             10316..12031
                     /locus_tag="LRB02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4660210.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALT
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="LRB02_002021"
     CDS             12034..13797
                     /locus_tag="LRB02_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4660211.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4065 a         2832 c         2402 g         4537 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg accaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgtttt
//
LOCUS       DAFFQY010000072        13537 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFFQY010000072 DAFFQY010000000
VERSION     DAFFQY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.072"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(60..1007)
                     /locus_tag="LRB02_002022"
     CDS             complement(60..1007)
                     /locus_tag="LRB02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL4660212.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="LRB02_002023"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="LRB02_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL4660213.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="LRB02_002024"
     CDS             complement(1642..2004)
                     /locus_tag="LRB02_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL4660214.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="LRB02_002025"
     CDS             complement(2015..3136)
                     /locus_tag="LRB02_002025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL4660215.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="LRB02_002026"
     CDS             complement(3152..3502)
                     /locus_tag="LRB02_002026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL4660216.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="LRB02_002027"
     CDS             complement(3632..5143)
                     /locus_tag="LRB02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4660217.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="LRB02_002028"
     CDS             complement(5465..6817)
                     /locus_tag="LRB02_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL4660218.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="LRB02_002029"
     CDS             complement(6970..8046)
                     /locus_tag="LRB02_002029"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL4660219.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="LRB02_002030"
     CDS             8242..9987
                     /locus_tag="LRB02_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL4660220.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="LRB02_002031"
     CDS             complement(10236..10703)
                     /locus_tag="LRB02_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL4660221.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="LRB02_002032"
     CDS             complement(10687..12060)
                     /locus_tag="LRB02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660222.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="LRB02_002033"
     CDS             complement(12381..12869)
                     /locus_tag="LRB02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660223.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="LRB02_002034"
     CDS             complement(12891..13493)
                     /locus_tag="LRB02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4660224.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4278 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcctt
//
LOCUS       DAFFQY010000073        13333 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFFQY010000073 DAFFQY010000000
VERSION     DAFFQY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.073"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="LRB02_002035"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="LRB02_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL4660225.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="LRB02_002036"
     CDS             complement(740..1462)
                     /locus_tag="LRB02_002036"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL4660226.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="LRB02_002037"
     CDS             complement(1598..2479)
                     /locus_tag="LRB02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL4660227.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LRB02_002038"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="LRB02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL4660228.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="LRB02_002039"
     CDS             3719..4198
                     /locus_tag="LRB02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL4660229.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="LRB02_002040"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="LRB02_002040"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL4660230.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="LRB02_002041"
     CDS             5499..5627
                     /locus_tag="LRB02_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL4660231.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="LRB02_002042"
     CDS             5631..6176
                     /locus_tag="LRB02_002042"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL4660232.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LRB02_002043"
     CDS             complement(6628..8319)
                     /locus_tag="LRB02_002043"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL4660233.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYAFNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LRB02_002044"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LRB02_002044"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL4660234.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LRB02_002045"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LRB02_002045"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL4660235.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LRB02_002046"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LRB02_002046"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL4660236.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LRB02_002047"
     CDS             12601..13302
                     /locus_tag="LRB02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4660237.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3751 a         2711 c         2367 g         4504 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat taaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taa
//
LOCUS       DAFFQY010000074        13182 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFFQY010000074 DAFFQY010000000
VERSION     DAFFQY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.074"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="LRB02_002048"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="LRB02_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL4660238.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="LRB02_002049"
     CDS             1995..4661
                     /locus_tag="LRB02_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4660239.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="LRB02_002050"
     CDS             5025..5777
                     /locus_tag="LRB02_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4660240.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="LRB02_002051"
     CDS             complement(5799..6953)
                     /locus_tag="LRB02_002051"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL4660241.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..8373
                     /locus_tag="LRB02_002052"
     CDS             7138..8373
                     /locus_tag="LRB02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBL4660242.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKSFISLRHLNGAVN"
     gene            8373..9578
                     /locus_tag="LRB02_002053"
     CDS             8373..9578
                     /locus_tag="LRB02_002053"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL4660243.1"
                     /translation="MEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQN
                     FQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVI
                     NAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGA
                     NMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNKEIGEQI
                     AKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQ
                     GLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQVIAAVG
                     LAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAI
                     QILEKIREK"
     gene            complement(9908..10612)
                     /locus_tag="LRB02_002054"
     CDS             complement(9908..10612)
                     /locus_tag="LRB02_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660244.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10799..11044)
                     /locus_tag="LRB02_002055"
     CDS             complement(10799..11044)
                     /locus_tag="LRB02_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660245.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11061..11912)
                     /locus_tag="LRB02_002056"
     CDS             complement(11061..11912)
                     /locus_tag="LRB02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4660246.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12078..13082
                     /locus_tag="LRB02_002057"
     CDS             12078..13082
                     /locus_tag="LRB02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL4660247.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4208 a         2341 c         2754 g         3879 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aagttttatc
     8341 agcttacgcc atctgaacgg cgcagtcaat taatagaaaa aaatgtattg acacaagaaa
     8401 ctgcgttgat ttttcaggaa caaacacttt ctgaagagct ctccgatcac atgatcgaaa
     8461 accaagtgag tgaagtggaa atcccaatgg ggatcgcgca gaattttcaa atcaatggca
     8521 agaaaaaatg gattccgatg gcaacagagg aaccatctgt cattgcagct gcaagtaatg
     8581 gtgcaaaaat ctgtggaaat atctgcgcag aaacacctca gcggctgatg cgagggcaaa
     8641 tcgttctatc aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat catcgaaaag
     8701 aagagctgat tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg
     8761 ttcaagatat ctctactcgg gaattcatgg ggagtttcca tgcatactta tctattgatt
     8821 ttttagtgga tgtaaaagat gcaatgggcg ctaacatgat caactcgatt cttgagagtg
     8881 tggcaaacaa gttacgggag tggttccctg aagaagagat tctattcagt attttaagta
     8941 attttgctac agaatcattg gcttctgcct gttgtgaaat cccattcgaa cgcttaggga
     9001 gaaacaaaga aatcggtgaa caaattgcca aaaaaatcca acaggcaggg gaatatgcga
     9061 agcttgaccc ttaccgtgca gcaacgcaca acaaagggat catgaatggt atagaagctg
     9121 tagtggcagc tacaggaaat gatacacgtg cggttagcgc ttcgatccac gcctacgcgg
     9181 caagaaatgg attataccaa ggattaacag actggcagat caaaggggat aaactagttg
     9241 gaaaactaac ggtgcctttg gctgttgcca cagtaggcgg agcttccaat atcctgccaa
     9301 aagcaaaagc atctttagct atgttagata ttgattcagc caaggaacta gcacaagtaa
     9361 ttgctgcagt agggttagct caaaacctgg ctgctttgcg tgcattagtt acagaaggaa
     9421 tccaaaaagg ccacatggga cttcaagctc gttcattagc gatctcaatt ggggctattg
     9481 gtgaggaaat cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga
     9541 cagctataca aatattggaa aaaatccggg aaaaataaaa aagcaatatc tttagaagaa
     9601 taaagcaaga tttacagtta ttttatttac tgcagatctt gcttttttct ctatcagaaa
     9661 atatgtttat tattagaatt ttttatagtt aaggttaggc atcttaagaa atcaggcaac
     9721 tccaagatat cttcaggtag aagagaagtc gcctaacatt gtaggatcat aggtgatttg
     9781 ttggacaggt caagctgata tttgagcctg atgttagaag gaatttcttt ttacttgctg
     9841 tagaaattat tatatgacta aaaaggctgg gattttaaat cccagccttt tatgacaaat
     9901 agaattttta tcgatcagta atcgctgtcg ctgcaaattg ttcgatcaaa ggaatcaaag
     9961 tcaatttgac tttgtatttc cgttcgagtg ctttttcgat aacgaactca ttttcagtag
    10021 tcatagaaat agttgctaat ttacgaggtt catcagcatt tttttcatta ataactactt
    10081 catgttcatt ttgttctgtg taaaatttag gatactcagc aacggctttt gaaaaatcag
    10141 caaaacttaa agcggcattc gtatcagttg agcgtgtatt ttcagttgtg tccgcttttg
    10201 gtttttccgt tttttcttct ggcacactga ctgtttcttc tgtaactggt gtcgcaacag
    10261 cttgcgcagt tggttcatcg tttttcaatc gttgatcatt ccagtaagac acgatttcaa
    10321 taagaagatt gtcacggaat cgttcgaaaa tcccgttttc tcctgaacga attctcttta
    10381 atacagtatt gaagttgtca tcgattggca cgttgatccg tgtatcttca tttgttttcc
    10441 cagctaatcc gatcgttaag atttgacggt ttttgggatc aagcatgact tgtaacggca
    10501 aaccataacg tcgcaagctc tcttcttcta gagaacggaa taattgtcct cttacaccat
    10561 ccaatgaagg acggaattga tttaataatt tttgtttctg ttttttcttc atatgttctc
    10621 tccttcgctc ataccctttc gattataaga aaaaaacagc caaatgaaaa ggagtaatgc
    10681 aaaaaaactt gcgtatacgc aaattacaac agaaaaattt gcgtattttc ccgttttttg
    10741 aaagaaaaat aaaaaatgga acggattatg tatatataca taatccgttc cattctttct
    10801 attttttcgg tatcaaggca aaaacaggta gttcaggtga ataaatgact tcatgttcgt
    10861 gccagggttt gaccattagc gaatgatcga accgtttttt cagttcttcg atgatttgat
    10921 catgtgtcca gtttcttgca ggaaaatgtt ggattttctc tgcatcgatc aaaaagacaa
    10981 aatcggaatg aaaagtctgg gtaacttctt ccgagagaga attcaagcgt tcgattggat
    11041 ccataaaatc acctttcgac ttaaaattgg gtagtatctg ggtcttttgc tttgccgact
    11101 acaccatcta actgatcgat ttgtttcatt tcttcttctg ttaattcaaa atcaaataat
    11161 tcagtatttt ctttgattct gcttggtgtc actgacttag gaagaggaag gaattcgtgt
    11221 tgcagcgacc aacgcaaagc aacttgtgca atcgtcttgt catgtgcttc agcgatttgt
    11281 ttcatttcag gcacatcgaa gattttacca gtgcctaatg gactatatgc ttcgaggatc
    11341 atatcatgtt tttttgcata ctcaactaca gttgtctgta attcgcctgg tgcaaggaaa
    11401 acttgattga ccatcggcat aatttttgct gtttttgcta gtgtatctag atggtggggt
    11461 aagaaattgc tcacaccgat tgctttgatt ttacctgctt catacagttc ttcaaatgct
    11521 cgccatgtat ctgcatttgc ttgttcccaa ttatcacgaa aagcaacagg gtttggccag
    11581 tggatcaaaa acagatctaa ataatctgtt tggagcttct tcaatgattc ttcaaaagaa
    11641 gacatcacaa gttcatagct gtggtttgca ttccataatt ttgttgtcaa gaatatctct
    11701 tctctaggaa ttccactttc cttaatcgct tggccgacgc tttcttcatt cttgtatcct
    11761 tgggcagtat cgatatggcg ataacctgca gccagcgctt ctttaacaga ggaaacagcg
    11821 acatccccat caggtgtttg ccatgtgccg aatcctacta ccgggatttc gtaaccattt
    11881 gatagacgat aagtgcttgt taatgaattc atactttcag ctcctttttt attcgttcaa
    11941 ctataagtgt agcgcatttt tcgaaaaaaa gaatccattt tgtttctact cttgaaattt
    12001 ttgtttaaaa gaaaatgatt ttgattctgg tatttttgat tttttttccg taaactaaga
    12061 cagaagggaa tgagaaaatg gatatctatt taaaaaaagc aattctgcat attattgaca
    12121 gagaaactgg ttctccagtc ttttcacaaa aagagctgga tctgacaaaa gaatatattc
    12181 gtgattttct tcaaaagaag atccaaaaaa tctcttctgc tcaaacaaaa actggacaac
    12241 tagcagaaga aagcatgatc gttgaatcac ttaaaatcgc tcaagaaaat tttgtggaag
    12301 caagtgaagc aatcgttcag cggtggttcg atatctacca agaaagtgaa gaagcaccta
    12361 gtgcagatgt atttgttgtc ttgtatgaat tagatacagt gatgtactta tctttattga
    12421 aagtcaatta tcgtgaggct tacactcact ttgttgaagc agacgaagca gggatcgaca
    12481 accgcttgat tttgaatcga gcaattctag gggcgaaaac acaaaaagca gatgaagcat
    12541 tcgctgtgaa tttgactgat tggacctacg aattaattga aaaaaaatat gagttttctg
    12601 gcaaaaaaga gctttatttt tctggaagag tgatcgaaag tgttcctgca ccttctttag
    12661 aagaaaatgt aaaagtgatc caaaaagtag ccaagcatct tggaaaaaaa tttgagacag
    12721 aagaatttga tatcatggct gatttgaaag aagctgtcta tgatacgata gaagaaaaag
    12781 gtcggttgga tcatgagatg attgcggaaa aagtcttcaa ggaaaacatc acggcaaaaa
    12841 tggcttttaa agaagaagta caagagcaag gatttgtccc agaagcacct cctgtaaaag
    12901 aagtacaaga aatttctgag aaaaaatttg gaaaacaaaa attcaagcta tcaaacggga
    12961 tcgaactgat cgtaccagta gatgtatatc gcaacccaga tttgattgaa tttgtgaata
    13021 atccggatgg tacgatatcc gtgatgatca aaaatgtgga tgaagtattg aatcgtttat
    13081 aaaaatgact agaatattag gtgttcaagt accagtgatc agttaaatga attgtgtcaa
    13141 gagaaggatc ggaaaagacg aatataacaa aaaaagagcg tg
//
LOCUS       DAFFQY010000075        12904 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFFQY010000075 DAFFQY010000000
VERSION     DAFFQY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.075"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..531
                     /locus_tag="LRB02_002058"
     CDS             <1..531
                     /locus_tag="LRB02_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4660248.1"
                     /translation="QAIAREASKYINDYDTLFINSSSTALKTLDFLQSNHLTVITNNV
                     KINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTISSVNADICIIGCSGVDVVHGVT
                     TKILNESTINEMMVNKTIKCKILVADHRKIGLTSKFKIADISVFDYLITDKFCSEKVL
                     EEIRNTGIKTIQVDSF"
     gene            complement(804..1631)
                     /locus_tag="LRB02_002059"
     CDS             complement(804..1631)
                     /locus_tag="LRB02_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660249.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            2199..3434
                     /locus_tag="LRB02_002060"
     CDS             2199..3434
                     /locus_tag="LRB02_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL4660250.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(4433..4693)
                     /locus_tag="LRB02_002061"
     CDS             complement(4433..4693)
                     /locus_tag="LRB02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL4660251.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(4720..6021)
                     /locus_tag="LRB02_002062"
     CDS             complement(4720..6021)
                     /locus_tag="LRB02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4660252.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(6313..6771)
                     /locus_tag="LRB02_002063"
     CDS             complement(6313..6771)
                     /locus_tag="LRB02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4660253.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(6784..8115)
                     /locus_tag="LRB02_002064"
     CDS             complement(6784..8115)
                     /locus_tag="LRB02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL4660254.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(8126..9568)
                     /locus_tag="LRB02_002065"
     CDS             complement(8126..9568)
                     /locus_tag="LRB02_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4660255.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(9555..10319)
                     /locus_tag="LRB02_002066"
     CDS             complement(9555..10319)
                     /locus_tag="LRB02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL4660256.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(10423..11298)
                     /locus_tag="LRB02_002067"
     CDS             complement(10423..11298)
                     /locus_tag="LRB02_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4660257.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(11553..11637)
                     /locus_tag="LRB02_002068"
     tRNA            complement(11553..11637)
                     /locus_tag="LRB02_002068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11601..11603),aa:Leu,seq:caa)
     gene            complement(11655..11726)
                     /locus_tag="LRB02_002069"
     tRNA            complement(11655..11726)
                     /locus_tag="LRB02_002069"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11692..11694),aa:Gln,seq:ttg)
     gene            complement(11736..11808)
                     /locus_tag="LRB02_002070"
     tRNA            complement(11736..11808)
                     /locus_tag="LRB02_002070"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11773..11775),aa:His,seq:gtg)
     gene            complement(11870..11954)
                     /locus_tag="LRB02_002071"
     tRNA            complement(11870..11954)
                     /locus_tag="LRB02_002071"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11918..11920),aa:Leu,seq:caa)
     gene            complement(11975..12045)
                     /locus_tag="LRB02_002072"
     tRNA            complement(11975..12045)
                     /locus_tag="LRB02_002072"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12011..12013),aa:Cys,seq:gca)
     gene            complement(12091..12162)
                     /locus_tag="LRB02_002073"
     tRNA            complement(12091..12162)
                     /locus_tag="LRB02_002073"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12128..12130),aa:Gln,seq:ttg)
     gene            complement(12174..12246)
                     /locus_tag="LRB02_002074"
     tRNA            complement(12174..12246)
                     /locus_tag="LRB02_002074"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12211..12213),aa:His,seq:gtg)
     gene            complement(12249..12322)
                     /locus_tag="LRB02_002075"
     tRNA            complement(12249..12322)
                     /locus_tag="LRB02_002075"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12288..12290),aa:Trp,seq:cca)
     gene            complement(12329..12409)
                     /locus_tag="LRB02_002076"
     tRNA            complement(12329..12409)
                     /locus_tag="LRB02_002076"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12373..12375),aa:Tyr,seq:gta)
     gene            complement(12411..12486)
                     /locus_tag="LRB02_002077"
     tRNA            complement(12411..12486)
                     /locus_tag="LRB02_002077"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12451..12453),aa:Phe,seq:gaa)
     gene            complement(12522..12594)
                     /locus_tag="LRB02_002078"
     tRNA            complement(12522..12594)
                     /locus_tag="LRB02_002078"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12559..12561),aa:Asp,seq:gtc)
     gene            complement(12604..12676)
                     /locus_tag="LRB02_002079"
     tRNA            complement(12604..12676)
                     /locus_tag="LRB02_002079"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12641..12643),aa:Val,seq:tac)
     gene            complement(12692..12763)
                     /locus_tag="LRB02_002080"
     tRNA            complement(12692..12763)
                     /locus_tag="LRB02_002080"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12728..12730),aa:Glu,seq:ttc)
     gene            complement(12778..12867)
                     /locus_tag="LRB02_002081"
     tRNA            complement(12778..12867)
                     /locus_tag="LRB02_002081"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12831..12833),aa:Ser,seq:gga)
BASE COUNT         4146 a         2693 c         2047 g         4018 t
ORIGIN      
        1 caagcgattg ccagagaagc tagtaaatac attaacgatt atgatacgct gtttatcaat
       61 tctagctcga ctgcgttgaa aacactggat tttcttcaat ctaaccattt gacagtcatt
      121 acaaacaacg tgaaaatcaa ttactctccg cacaaagata attgtagtta tattttaact
      181 ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg acatcgcatt aaatacgatt
      241 tcttcagtca atgcagacat ttgtatcatt ggctgtagcg gagtcgatgt agtacatggg
      301 gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa tgatggtcaa caagacaatc
      361 aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac tgacatcaaa gttcaagatt
      421 gctgatattt cagtctttga ttatttgatc actgataaat tttgttctga aaaagtatta
      481 gaggagatca gaaatacggg gataaaaacc attcaagtag attcatttta agaaccagga
      541 cgtaaatatg taaataggat tatcaaaaaa atgaataaca aatttatcga taaatgaaaa
      601 acacgctacg ctcaaattga taaaggaatc ccaagatgta tccatccaat cgataccatc
      661 ataatctatt gcttctttta taaccgttat atgttgccta aataggaatc aaacaatatc
      721 gaaaaaagga ggtatgacaa aagtcacacc tccttttttc gaataaacgg tattcaaatc
      781 agaacactga tttcacaacc actttatttc aggtcgtttt caatcacctt taccgcgtaa
      841 tcatttccgt tgaaacgcgc tatttttttt gcgtccatat atctttctaa ataatcatct
      901 tctcctaatt gcttatggca agaagcaatt tcataaagca agtattccaa tgcataagtc
      961 gatttatgtt tcttattata ctcaataccc ttctcacata attggattgc tttatcataa
     1021 tcctcgattt tacggtaata ggtggcgata tcataatata caatgcagac catcagatta
     1081 tcaaaatcag tctcactcaa tattttaagt gtacgctcca cataaacttt cgcaaaatct
     1141 aattctcctt ttgacaagta gatcattgcc acgtttgcag ctgcttgtaa gtgatggata
     1201 tctgtttcgg tcgtttgttc caacacttga ttaaaataaa ataatgctgc atctggtttt
     1261 ttcgcataaa gatgatcaag cagtccttga taaaataaaa tttgatttaa aagatcagaa
     1321 ttacttactt tttcctgatc gatcgtttgt aaaattttat aagattcttg gtattttgat
     1381 acagaattca atgattgaat ctcatttatt tttttcctga ttttgtcttc ttctgtttct
     1441 actaatatat cggagatctc gacattcagc tttttgcaaa gcgcacttaa gatattgatg
     1501 cctggcgaca tattcttgtt ttcgatattg ctgacagttg cttgttcaca aatgccttct
     1561 gctaattgta cttgtgttaa cccctttttc tttcggtatt cttttagtaa tgctccatct
     1621 actttaatca aaacaatttc cttcccttta aaaaattatt tctcctttat atcatacaca
     1681 aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat aaaaatttta attttaaaat
     1741 aatattcaaa ttaaacaact ggcgtatctg ctccttgatt tctttttgtg ttatctgaat
     1801 aattatcgtt tacagttata acacatagtg aaaattgtca tggcgagaaa gtctaagaat
     1861 atttttaaac ataactattt ctttcttacg tgtgtataat gacgacagca cacctatcaa
     1921 ttaatataat tattttattt ttttatgttc tcatgtttat ttttaatgac acaacttctc
     1981 gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt ttttgaaaat
     2041 tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag cgagagacag
     2101 tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta tactagtaag
     2161 aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt ctttttgctc
     2221 ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa gctatgaaag
     2281 accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg atcacagaaa
     2341 gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa gaacaaatca
     2401 taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg gttgcctctc
     2461 gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt gtgacaggtt
     2521 cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta tatgttaaac
     2581 cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc cacgagttac
     2641 gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt atagtaaata
     2701 cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt cctttaccaa
     2761 aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct aaactatttt
     2821 tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt gctaagctca
     2881 gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa gaaagaaaac
     2941 gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc aaaattttgt
     3001 tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat ctgcttgatg
     3061 acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt catcttttgc
     3121 atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa actgcctgtg
     3181 aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt aatattgact
     3241 attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa agaaaactgc
     3301 agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc acgctgcaag
     3361 ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc ttcctgagca
     3421 accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat cagtaatttc
     3481 gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt gaaaagttag
     3541 agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa aagatctgac
     3601 gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt ctttgacacc
     3661 tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata attaggcaaa
     3721 tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga gatttgtgcc
     3781 gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat ctttgataat
     3841 caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc ctatataaac
     3901 tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc gtcagcaacg
     3961 atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat tatttctata
     4021 cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat acaccatact
     4081 gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat cactttggta
     4141 gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc tccttctttc
     4201 tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga caaaagcctt
     4261 gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg gtaataagtt
     4321 caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt ttcgtaaatt
     4381 tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac atttaaagct
     4441 tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa attccacaaa
     4501 gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc tttggaagaa
     4561 tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata tgatatgtag
     4621 ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca aaaaataaaa
     4681 tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct ggacttcgtt
     4741 ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta caaatggata
     4801 atagataata aatgctatca atgtgttgat catgatcaag ataacaacac gccagtcatt
     4861 tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca aaacagtaaa
     4921 tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa ctaaaggaac
     4981 taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa aaattatggg
     5041 ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt tcaagaattt
     5101 tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg caccaccaat
     5161 tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg tggcagctgt
     5221 accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg gacgaacaac
     5281 agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga tcaaaaacat
     5341 tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct cgaataacga
     5401 taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag taattgttaa
     5461 aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg gcacttgttc
     5521 tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca ctgcaaaaat
     5581 catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag gtaagaccca
     5641 gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca taaagaacgt
     5701 tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag ctagatccga
     5761 acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa agggtaaaac
     5821 aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta aattgataaa
     5881 gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc cattacgaat
     5941 tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt tctcttcgac
     6001 ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga accattcatt
     6061 gcatgacaac gcaaagaatg ttcataacta attatataat taagaaattt tatttcaata
     6121 tattctctta aataatcaaa tttattttta agaatcctga aaataagttt tctaaatcat
     6181 aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc tagtgattga
     6241 aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt
     6301 tttcatcgta cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt
     6361 taataaattt ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc
     6421 ttgctccaac ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt
     6481 actttcaacc cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg
     6541 gatcagtatt tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat
     6601 attatcgatt attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta
     6661 tccaactcct atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat
     6721 ttgaccacta gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca
     6781 cctctacttt aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt
     6841 ttttgcgact ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg
     6901 ataagaacct tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat
     6961 caatccttta tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc
     7021 accaacatat gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg
     7081 attaggaacg attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc
     7141 gacgatatat tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg
     7201 gcgtatttta tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc
     7261 acgtgtatac tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa
     7321 gtaattattc gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt
     7381 tttttgccaa gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag
     7441 tttttcaata atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata
     7501 ccagccaaag tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta
     7561 attcactgca atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac
     7621 attcggatat ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa
     7681 aatccatgct ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat
     7741 tgagcgcatc ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg
     7801 tttaagtgga atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga
     7861 gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat
     7921 atttgaaaaa ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct
     7981 ttcttcatca atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa
     8041 aatggcttga acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg
     8101 ttttttcatc atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa
     8161 cataagtcac ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc
     8221 cgcttatccc taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta
     8281 cccgtaggcg tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt
     8341 gtagcatttt tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt
     8401 ctaaggttac ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc
     8461 tcttattgat caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg
     8521 gaagaatcaa aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg
     8581 ctccgaatgt aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg
     8641 caaaaagtgc atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag
     8701 gctcagtgat atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca
     8761 tctctttatt ttctttataa gctgctttat agatagctag acatatagct ggtataccaa
     8821 acatcattac tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa
     8881 agcgtgtcat ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt
     8941 aataaatata cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa
     9001 acataaatat ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa
     9061 tcccaatttg gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa
     9121 tcatcaccat agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt
     9181 tttgttcttt aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa
     9241 aaatccccat attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg
     9301 ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa
     9361 ctcctatagc aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta
     9421 ctgggatgtt cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg
     9481 ctgcaactgg aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt
     9541 ttattcctcc tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc
     9601 actttttgag caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca
     9661 aaacttgaac catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc
     9721 gttttatctt ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg
     9781 atttgtcgtt ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg
     9841 acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt
     9901 gctaatgtaa caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct
     9961 aaatgagttg gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga
    10021 tacagttgat ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct
    10081 ccgctttcga taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa
    10141 tggactccta cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc
    10201 ggcatatttg ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc
    10261 ccataattca caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt
    10321 tcattctcct tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact
    10381 atgtgtcatt tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag
    10441 atgaattgcc aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat
    10501 gatatcttta atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa
    10561 aaaaccaata ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt
    10621 gatatgaacc attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga
    10681 tttttctaga tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact
    10741 attaggctct cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga
    10801 ccctaactcg gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata
    10861 ttcatttgga taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc
    10921 tctttttcgt tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc
    10981 taatggagag gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa
    11041 ttcgattgga atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa
    11101 tacaaataat ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc
    11161 aatccctttc cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg
    11221 attcagcttt ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca
    11281 acttgcagac tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga
    11341 cctaagaaag aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt
    11401 acaaaaatag tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga
    11461 taatacttgt atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac
    11521 tctctataag aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg
    11581 tcctcaacgg acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa
    11641 tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc
    11701 ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac
    11761 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga
    11821 tgttatttaa tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc
    11881 ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat
    11941 tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg
    12001 atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat actattatca
    12061 atctataaaa taaagaataa attatagaaa ctggggtagc tggattcgaa ccaacgcatg
    12121 agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa aagggcgaat
    12181 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
    12241 attcgccatg gcaggggcag caggaattga acccacacta acggttttgg agaccgctgt
    12301 tctaccttta aactatgccc ctataaaatg gaggagagtg gattcgaacc actgaaccct
    12361 aaggaacgga tttacagtcc gtcgcgttta gccacttcgc tactcctcca tggtggcgcg
    12421 ggacagaatc gaactgccga cacacggagc ttcaatccgt tgctctacca actgagctac
    12481 cgcgccatat tttaaaatta ttaattgaat aacaagaacg acggttccga cgggaatcga
    12541 acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccagttca
    12601 ttatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
    12661 gctgagctaa acctccaaca ttcaattaaa atgacccgta cgggattcga acccgtgtta
    12721 ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa tttgctacgg
    12781 agagtaaggg attcgaaccc ttgagacagt ttggcaccat ctacatgatt tccaatcatg
    12841 ctccttcggc ctctcggaca actctccaga gtgaaagaag ctcaatactc tttctcaaaa
    12901 tgag
//
LOCUS       DAFFQY010000076        12829 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFFQY010000076 DAFFQY010000000
VERSION     DAFFQY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.076"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(151..840)
                     /locus_tag="LRB02_002082"
     CDS             complement(151..840)
                     /locus_tag="LRB02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660258.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(815..2944)
                     /locus_tag="LRB02_002083"
     CDS             complement(815..2944)
                     /locus_tag="LRB02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL4660259.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(2992..4230)
                     /locus_tag="LRB02_002084"
     CDS             complement(2992..4230)
                     /locus_tag="LRB02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL4660260.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            complement(4582..4941)
                     /gene="rplT"
                     /locus_tag="LRB02_002085"
     CDS             complement(4582..4941)
                     /gene="rplT"
                     /locus_tag="LRB02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL4660261.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(4994..5194)
                     /gene="rpmI"
                     /locus_tag="LRB02_002086"
     CDS             complement(4994..5194)
                     /gene="rpmI"
                     /locus_tag="LRB02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL4660262.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(5266..5790)
                     /gene="infC"
                     /locus_tag="LRB02_002087"
     CDS             complement(5266..5790)
                     /gene="infC"
                     /locus_tag="LRB02_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL4660263.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(5834..5959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6110..6742
                     /locus_tag="LRB02_002088"
     CDS             6110..6742
                     /locus_tag="LRB02_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL4660264.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(6744..7649)
                     /locus_tag="LRB02_002089"
     CDS             complement(6744..7649)
                     /locus_tag="LRB02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4660265.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7734..8393)
                     /locus_tag="LRB02_002090"
     CDS             complement(7734..8393)
                     /locus_tag="LRB02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4660266.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            8709..9653
                     /gene="mvk"
                     /locus_tag="LRB02_002091"
     CDS             8709..9653
                     /gene="mvk"
                     /locus_tag="LRB02_002091"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL4660267.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            9646..10623
                     /gene="mvaD"
                     /locus_tag="LRB02_002092"
     CDS             9646..10623
                     /gene="mvaD"
                     /locus_tag="LRB02_002092"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL4660268.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            10636..11721
                     /locus_tag="LRB02_002093"
     CDS             10636..11721
                     /locus_tag="LRB02_002093"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL4660269.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            11718..12782
                     /locus_tag="LRB02_002094"
     CDS             11718..12782
                     /locus_tag="LRB02_002094"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL4660270.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         3778 a         2727 c         2433 g         3891 t
ORIGIN      
        1 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt
       61 gaatattttg acttgtgacc gaggagttga caggagaaca ccgtttattc ggaaaaaaag
      121 gactgtgaca cgacttttgt cacagtctct ttatttgtaa tctaaatcat actccgtaca
      181 tcttttttct atttctttta tttcttctaa gatcccttgg ctacgaattt cattatatgg
      241 ccaaagtaag actgcctcta atacataagg cgaaaaatcg ctttgattca tctcttttga
      301 aaaactcatc gctgctatct cgctcaatgc acgaatgttt gattgatgtg tataaaaagg
      361 caaccgccac agcacaattt tttcgctttg cgtataaatt cccttaacag aagcttcgat
      421 gtggtgaaat agttcatgtc caataatcaa agcttgcgct gtttctttag agatcgaagc
      481 tagtcctgaa cattttaatt tttctaacgg ctccgtcatc aatcggatcc cttcatcttc
      541 tgcataagtt gcaaaagtga ttcgttcgtt tatttgctct gattcacagg aaacgattgg
      601 aacagccatt tgttcataga tttctcctac atcggcaaaa gggtactttt ttttgatgct
      661 acatgccgtt tcgataccac aatcgattga ttgttgtata agaattttct gcttggacaa
      721 aggaatcttt ttccaaaata aatctcgccg aaactggtag aatccccaaa tttcttcggg
      781 gatctgttgt aattcgttta gtaactcttc taacttatgt ttgtttgata tttccggcaa
      841 cgacgatcac ttcttcctcc ggttccatag tagatacttc cagatccatc aaaagctgta
      901 gttgttccag atccgtcgct gtaaaatcag agactcttgc tcctaagcag agataaaatt
      961 ctggtcgggc aatcaattca gtttgataaa ctgccatatc ttcccaaagt tgttcgacag
     1021 ctgtcatata attggctgca gttgttttca tacatgaagc atgtccacgt tggaccttga
     1081 taaatccttt ttgatcgatg atccgaagac ttccagcatt ttttgaacga ttctgttcgc
     1141 cgcatacata aaagaaaggt gtattttcca ataagcaaac ttcattttta tttaatcgca
     1201 tatcctctgc agcaagctct agtgcttctt ctgttgtgat ctccttggtt agatcggttg
     1261 ctttcacttc cgtagaacca gtagcgatag ccgttacttt tgaagtatgt ggatcgattt
     1321 ctacgtggac ttcgatcgat tctggtgtag cgcctgattc aatcgcttta ttcatcgctt
     1381 catttttcaa cgatcgtata tcttctttac ttggcgaagg gatgatacgt tctacaacat
     1441 ctcggaccat tgcaagtgct acaccaatag aagaaatcac ttcggcgttt tcaggaatcg
     1501 aatatttcac acccatttta ttactaaagt aagtaatcaa tgaggcagcg ccgcccccta
     1561 cacctacaag agaaatctga tctttctcca agtgatactt gtctgccaat tctaaaatca
     1621 ccggttcaat cttagcatag gatttttcca tgatctgctc tgcgatgtct tcaaccgtcg
     1681 tatgacaata gtctgctaat gcctgtatgg cttttcgtgc agatgggaca tttccataag
     1741 aaaaatgttc ttcttgcact aatccaagaa cattggctgc acatgtattt gtaatagtca
     1801 cttcttctcc gtcttccatc actaccttga cgtaatcagc agggtctcct tctttaggag
     1861 agaaaaattc aaccttagga cctttgattt tttctgtctc cgtaaatact gcataatcta
     1921 gaccggcaat atgtgcagaa cgtggaccga catcgatgat tcctgactga ttagcccgta
     1981 ccatcgaacc gcctgccaca cctagaaccc gaacatctaa tgaagagata tatgttgggt
     2041 ggccacctac aattgaataa tcgattgctg gacgtccatt tttaatgacc ccgatattgg
     2101 ttgttgttcc gcctacttca aaataaacac cattagacgc tctcaagtac atcaaagagc
     2161 ccatgactga tgcagcagga ccggaaagca tggttaaaac aggtcgtttt ttcatttcat
     2221 tgatttccat tactccgccg tctccgcgca taatcatcaa agaaacattg acccctgctt
     2281 ctcgtacgga gtcttctgtc gaggttgccg tatctagcat ttttggtaaa atagatgcgt
     2341 tgattgctgc tgtccgagtt cttcttgtca atccatataa ttttgtgatg tcagaagcca
     2401 tcgttgtagg gatcgctttc acagatgctg cttcataaac aactcgttct ggttccccat
     2461 tatctactcc aaatgccata gaagaaacca agacctccgc tctttctctt tctaaagaag
     2521 aaatcgtttc tgaaacacgg tctacattta agtttttgac aggtaagaat gcattgacaa
     2581 tctctatttt tttcttattc cctaaatcga tatcgttcaa tctagtttgt cgttttgcta
     2641 aaaaaccttc taatccacct tttgccatcc cgattacgcc gacttttgct acatctcctt
     2701 caatcagtgc attagtcgct tgtgtggtag aatgtgcaac aaacacaaca tcttctggac
     2761 tgatattatt ttcccgcaaa caattttgaa aagactgaac gacacctgcg gctactccac
     2821 ggacatcatc gtgggttgtt ttcactgaag attttccgat gatttcgtgc gtcgcattat
     2881 cgattgcgac tgctttcgta tgtgtaccgc caacatcaat tcccattcgc acaaaacgtt
     2941 tcattttctt ttcctccatt tatctaatcg tttttatcga cgttttgttt actaggcgtt
     3001 cccgtacaat aagaacgtga tcaggcataa gatcgcaccg atgatccaac cagttgggat
     3061 ggatttcttc atatattctc gagaagttac ttttgtataa cctaatcccc aagcaaccca
     3121 agattgtgta atatctaaat gctgaggagc aatcgttgta attgcaaata acgggaacaa
     3181 aaaggctggc gacatcgttg gattaacagc taacacgaca gctaaaatag ctgagcctga
     3241 accgactaga ttcattggac cgcggaaaaa tcctagtggt gtcaatatag caaatacgat
     3301 tgccaagact aatggtgatt gcggtatcca atctccaaaa atagctgaaa aataaggaga
     3361 agcatacgca gctgaattat tgaacatcgc taaggtcatc aagaaaccaa ccattggtgc
     3421 aacatcgact gcgccatctg taaataattt cgcaaactga cgacagattt ctttataacc
     3481 tccgcgcaat tttccagtaa acagcaatgc ccagatacct gctagaataa aacctaagat
     3541 gatttgaaga tctaaaagaa cgacccctaa aactggtaat aatacggcta accatgagat
     3601 cattggtgcg tcgccacctt cagctggtac gtttgctgcc attgcatagc gtttcttttt
     3661 gttcattgat atattagcga ccgttagtac cacaactaag cttacaatca tcccaatcat
     3721 accaatttgg aaatagtcat tataagtata gctttcagcc gctggattga aaccagcata
     3781 aatcgtttga tattgtttga aattgacgat attagcaaag attccagcca taatcgagcc
     3841 cataaatgag aacagtgcaa ccggtactga aatacctaat gacatcaaga tcggcaacac
     3901 aataactgca atagaaatca ctggtccgat tcctgtcatc gacatgaata taacagcggt
     3961 tacaatacat agtaagctca tcgtgattct tggcttgtcc cctccaagtt caactacctt
     4021 tcgaatcagg gtagctgcga ttccgctatc tactagaaca cgcccgaaaa aggcaccaaa
     4081 gaagacattt accaagatcg atttggcgta ctcagctggc cccgtcgtat agatatgcgt
     4141 caacacatca attactcctt gcccttccat cgccgagttt ggtgaaaacg tgtttccgac
     4201 tagcgctaat cccatccata cagtagccat gatgacaaaa gccgaccatc aagttgtagc
     4261 cttttacaca gtaccagacc atgaaaaaga atgagataac taacaaaata ccaataataa
     4321 tatcactcat gttattcctc cgtccatttt gtttttctga taagcaaact aatatatcag
     4381 tcaaactcat tctaacttat gtagttagcg ctttcattaa aggatgacca taaatgttca
     4441 gttttcaatt aaaaaacctt aattatgtta ttttagactt atacgttatt gtcctgtttc
     4501 atttgatgga ggattgtaaa taaaaaaaga ggaaagaaca taacttttcg ttatcttctt
     4561 tcctctttat aattgtttag cttatttagc caaagcttct tttgcttggt cagccaatgc
     4621 agtaaatgct gctgcatcgt taacagccaa gtcagctaac attttacggt tgatgtcgat
     4681 ttcagcaact ttcaaaccgt gcattaattt tgagtagctc aagccattca tacgagctgc
     4741 tgcgttgata cgagcaatcc acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg
     4801 atatgcgtag ttgtatgaat tcattacttg ttcttttgct gttttaaata aagtgtgttt
     4861 tgaaccgtag taaccttttg ctaattttag cactctttta cgacgtttac gagttactgt
     4921 tccaccttta acacgtgcca tgtataattc ctcctaataa aaataaatct tcagattctg
     4981 ttatttgtac ttcttatttc atttttgcta gttgttggcg aatacgtttg aagtcgccac
     5041 tagaaaccat gcttgcttta cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac
     5101 ggtgacttgt aaacgcgcgg aagcgtttca atccgccttt accagtacgt ttgaaacgtt
     5161 ttgctgatcc gcggtgtgtt ttttgttttg gcatgactat atttcctcct caaaatcttt
     5221 cgttatccga ctatcggctg tccaacagcc agttagtcat tcaaattact tactgtcgtt
     5281 tttcggtgcc agcgtcaaga acatgctgcg tccgtccatt ttcgcttttt gttccactgt
     5341 tgcaatatcc gcagtttctt cagctaagcg attcaagact ttttgaccaa tttctttatg
     5401 ggtaatggca cggcctttga aacggataga agctttcact ttatcgccct tttccaagaa
     5461 cttacgtgca ttacgaagtt ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg
     5521 aacttctttt acattgatca ctttttgttt tttacgcgct tcgcggtctt tcttttgttg
     5581 ttcgaaacgg tatttaccgt agtccatgat tcgcgcaact ggtggtttcg caccaggggc
     5641 aactaacaca agatccaagt ttgcttgttc tgcaatattc aatgcttctg cttttgtctt
     5701 aactcctaat tgctcaccat ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc
     5761 gttaaccatc atatcctttg ctatggtcat tcacctccaa atattatgag agaaagaaaa
     5821 gcttaccgaa taaacggcaa taaaaaaacg agttctctta cagaacccgc gcgtacttca
     5881 cctaatagaa ccagtgaaaa ctatctagcc cagtgacatt atcaactaag gcgagaagcg
     5941 ggtgcttctg cttttatttc tcaaccatta tagtatagca aaaactaatc tttttgtcaa
     6001 tgtttttcat acagtttgtc ttccttttct gaaaatgatt agattagcag gctttcttgc
     6061 agtgattccc gcttctttgc tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat
     6121 cgaacctatt ccaccttctc ttcctcttct tacaaaggga gagttttatt tagaagatga
     6181 ggagatcttc tctggtcttc tgatcaacgg aggtaaccaa agctatctcg atgccaaaaa
     6241 tatggtatta agagagagtc atatccaaaa acttacgatg caaaaaacag gtttacgtca
     6301 ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt tccaatctgg agtggattgg
     6361 tggcagtttc caccaagtcg tgtttcatca atgtaaatta acaggtacga attttgccga
     6421 aagttatctt cgagactgta ctttcactga ttgtattgct tcgatggctt cctttagtag
     6481 tacaaacttg aaagctgtct ccttcgatca ttgtcaatta gaagacagcg aattttatga
     6541 ggtcacttgg aaaaaccttt ttttgtctga caatcagtta accggttcga actggtttcg
     6601 aacttctctg aagggattag actttacgac taatttcttc acaacaatcg ctttatctca
     6661 agattctttg gcgggactaa tcgtcaatca agagcaagca cttgtgatcg cccaagccct
     6721 tggtttgcag atcaaagact agatcaacga atccgccgcc acagtgcaat aagcatccaa
     6781 atagataaga ccaaactgat gatcaaggcg atttcccatc cgaaaacgga atgggtaaag
     6841 ggtaactgga gattcattcc gaagaatcct gtcacaatag ttggaacggt caagatcagc
     6901 gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt tattcagcaa attattataa
     6961 gtgccagaga gctggtctaa aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt
     7021 gcttcgatca gtgcatcgtc caactcttct ttttcctttt ctgacaactt gcgataaata
     7081 gccagtgcct tgatttgttc taaaagtact acattttgtt ttgttcctgt aactaagtaa
     7141 accaaaccga tctctaaatc tgaaagttgc agcagattct tgttcgttgt tttttctctt
     7201 aatttcagat taagccgctg acgttcacta ttcacttcct cgattaaagg aaaaaaggaa
     7261 tcagaaatca aaaacaatgt acgaaacaat aaactgtata cactcatgtc tggcatccgt
     7321 tccaaaagcg atcgaattag tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac
     7381 aaaccgtctt cttttaaaat aaaagcgata ggactagttt cataatgatt atcgactttt
     7441 tgctggtgcg gaacatttga tacaagcagt aacgcttcat caaaagaatc gtactctacg
     7501 cgcgcccgtt catttttatc tagagaataa gtcagcatct cgtcactaat gccatattct
     7561 tcttttaagt ttttaacagc tagcacatcg ttttgctgta cattgatcca aaaattacgg
     7621 tcttgattaa aatagtgttt ttcccgcata ctctatcctc ctgacatttg tttccagtat
     7681 aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt gagcaactgt aaatcaaatg
     7741 gatactttgg tttggcgaat ctctgctaat ttttcttcga tcattttcaa gacttcttct
     7801 tttgcttgag gatcttccac aaaattatag cggtctccat cgatttggat ttttggactt
     7861 tctttgtatt cttcatacca ttgatcataa cgtctgttca attctttgta ataatcgtat
     7921 agacttgggt caaaggacaa ttgttcatac gaacgaccac gtttttcgat acgctctaac
     7981 atcgtatcaa aagatacacg aatatgaact aataaatccg gatgtttttt atgggcagca
     8041 tagggaagtt cttccatcat gttttccagc agttcatcat acacacgaac ttctgtttct
     8101 gttgctcttc ctagatctgc atttagatgg aaaagcagtg aatcttcata aatcgaacgg
     8161 tccaatacat tattgtcatc tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa
     8221 aaataaattt gcagcaaaaa agcatactgt tcagggtttg cataaaataa gggcaagact
     8281 tcattatcat caatcgattc ataaaatgcc tgactatcaa agtgttcagc catcatttct
     8341 gttaaacttg attttccggc accaattgta cctgctaaaa cgatcacgct catttacctc
     8401 cagctcactt catcactaaa tattgtgttt aactccaacc ttttttaaat atacccccaa
     8461 tatctagtag tgtcaattgt attttttcta gtcaaagaag aaaaaaagtc aaaaaattct
     8521 taagaaaaaa gtgttttttc tttagaaaac gtattcttag ttttacttct gagatggttt
     8581 tagtaaaata aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc
     8641 agcgcaactc gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga
     8701 gttagattat ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg
     8761 agcacgcggt tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca
     8821 ccgcattcct tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag
     8881 gaaatttagc cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa
     8941 aaaaacagca tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg
     9001 aacgtggaat gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt
     9061 acttagcatt tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa
     9121 tcgcccacgg taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt
     9181 attttacaaa agggcatcct tttgactact tttctttgaa catcgatgct tttttgattg
     9241 tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct
     9301 ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc
     9361 aagcaaaaca agcaatcatt gagaattcac cagaaacgtt ggcacagaca atggatgaat
     9421 cacagtcact tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa
     9481 cagcaaaaga taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta
     9541 tgattgcatt agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg
     9601 cgggtgctgc tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa
     9661 gcacgcgcat ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc
     9721 atccttccaa tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa
     9781 gtcatctttt ctgatagcta tatggtagat gaattttatc tagatggcac cttacaagac
     9841 gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc
     9901 tcactaaaag cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc
     9961 tctgccagcg ggctagctgc tttagcagga gcttgcaata ctgctcttaa gcttggatta
    10021 gacgatctct ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt
    10081 ttcggtggtt tcgttgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca
    10141 gtcccttccg attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag
    10201 aaaaaagaag tgtccagcag aaatgggatg cgtcgaacag tcgaaacatc cagtttttat
    10261 caaggctggt tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca
    10321 aaagatttcc aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg
    10381 actttagctg cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat
    10441 gcggtgcggc aattgagaaa aaaaggcatt ccatgctact ttacgatgga tgctggccct
    10501 aatgtcaaag ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa
    10561 ttatttagca aagaacaagt aattactgcc catgctggtc caggaattgc gattattgaa
    10621 taaagggaga tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa
    10681 tatgcagttg ttgaaacagg acatccagca gttatcgctg cagtcgatca attcgtaaca
    10741 gtcactgtag aatccgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg
    10801 cctgtacgtt ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt
    10861 cattatatcc ttgctgctat tcgtttgact gaaaagtatg cacaagaaaa aaacattctt
    10921 ttatcctttt atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat
    10981 ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac
    11041 gcgttgaatt tatctcaatt ggagattttc aagattgcag cactagccaa tttagcagtt
    11101 caagataatg gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc
    11161 tcaaccttcg accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta
    11221 cttgccctgg attggccagg tctatccatt gagccattga ttgctcctga agatttacgt
    11281 ttattgattg gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac
    11341 cgttcgagag aagacaaaat ggtggcttat cagcttttct taaaaaacag tacagaatgt
    11401 gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt
    11461 cgaaaaaacc gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg
    11521 cctgctttga acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct
    11581 ggtgcaggtg gtggcgattg cggaatcgta attgttgacc agaaatctgg catccttcct
    11641 ttaatgagtg catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat
    11701 caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc
    11761 atgataaaca aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa
    11821 tagcagtttc tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat
    11881 tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg
    11941 caatcgtcgc aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga
    12001 aagacccttc tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa
    12061 tcattgccaa catcggtgca gggacttctg tcgaaagagc ccaagaagct atccgattgt
    12121 ttcacgcaga tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg
    12181 gagaccgtga ttttaccaat tggaaagtac ttattcaaga gactcaaaca gccattgacg
    12241 tccccctcat cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag
    12301 ctgctttagg agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga
    12361 tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga
    12421 cagtctcttc attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg
    12481 gaggtatccg caatgcctat gacatattta aagccctttg tctcggcgca aatgcagtgg
    12541 gtacttcagg aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac
    12601 tgatgaagca atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata
    12661 ccgcggccct acaccaacaa tcactgatct tttccggacc agtgaaggat tggtgcgaag
    12721 caagagggat cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt
    12781 aaagaacact tttatcccac gctctaaaca cgtataaacg gcggaagca
//
LOCUS       DAFFQY010000077        12673 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFFQY010000077 DAFFQY010000000
VERSION     DAFFQY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.077"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="LRB02_002095"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="LRB02_002095"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL4660271.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="LRB02_002096"
     CDS             1371..2546
                     /locus_tag="LRB02_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4660272.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="LRB02_002097"
     CDS             2550..3191
                     /locus_tag="LRB02_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4660273.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="LRB02_002098"
     CDS             3188..4105
                     /locus_tag="LRB02_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4660274.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="LRB02_002099"
     CDS             4102..4773
                     /locus_tag="LRB02_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4660275.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="LRB02_002100"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="LRB02_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="LRB02_002101"
     CDS             complement(6004..6270)
                     /locus_tag="LRB02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL4660276.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="LRB02_002102"
     CDS             complement(6270..6533)
                     /locus_tag="LRB02_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4660277.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="LRB02_002103"
     CDS             7008..7193
                     /locus_tag="LRB02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4660278.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="LRB02_002104"
     CDS             complement(7335..9158)
                     /locus_tag="LRB02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL4660279.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="LRB02_002105"
     CDS             complement(9755..12460)
                     /locus_tag="LRB02_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL4660280.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2637 c         2389 g         3996 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atc
//
LOCUS       DAFFQY010000078        12670 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFFQY010000078 DAFFQY010000000
VERSION     DAFFQY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.078"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LRB02_002106"
     CDS             6..305
                     /locus_tag="LRB02_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4660281.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LRB02_002107"
     CDS             378..1739
                     /locus_tag="LRB02_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL4660282.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LRB02_002108"
     CDS             1761..2021
                     /locus_tag="LRB02_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660283.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LRB02_002109"
     CDS             2035..2787
                     /locus_tag="LRB02_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL4660284.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LRB02_002110"
     CDS             complement(2881..3486)
                     /locus_tag="LRB02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4660285.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LRB02_002111"
     CDS             complement(3539..4513)
                     /locus_tag="LRB02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL4660286.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LRB02_002112"
     CDS             complement(4891..5610)
                     /locus_tag="LRB02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4660287.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LRB02_002113"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LRB02_002113"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL4660288.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LRB02_002114"
     CDS             7161..8558
                     /locus_tag="LRB02_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4660289.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LRB02_002115"
     CDS             8639..9079
                     /locus_tag="LRB02_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4660290.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LRB02_002116"
     CDS             9227..9553
                     /locus_tag="LRB02_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4660291.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LRB02_002117"
     CDS             9554..10303
                     /locus_tag="LRB02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660292.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LRB02_002118"
     CDS             complement(10383..10838)
                     /locus_tag="LRB02_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL4660293.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LRB02_002119"
     CDS             complement(11265..11540)
                     /locus_tag="LRB02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660294.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LRB02_002120"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LRB02_002120"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL4660295.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DAFFQY010000079        12060 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFFQY010000079 DAFFQY010000000
VERSION     DAFFQY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.079"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..197)
                     /locus_tag="LRB02_002121"
     CDS             complement(<1..197)
                     /locus_tag="LRB02_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL4660296.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
     gene            complement(204..434)
                     /locus_tag="LRB02_002122"
     CDS             complement(204..434)
                     /locus_tag="LRB02_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660297.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            complement(431..742)
                     /locus_tag="LRB02_002123"
     CDS             complement(431..742)
                     /locus_tag="LRB02_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL4660298.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            complement(881..1696)
                     /locus_tag="LRB02_002124"
     CDS             complement(881..1696)
                     /locus_tag="LRB02_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBL4660299.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(1693..2019)
                     /locus_tag="LRB02_002125"
     CDS             complement(1693..2019)
                     /locus_tag="LRB02_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660300.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            complement(2016..2177)
                     /locus_tag="LRB02_002126"
     CDS             complement(2016..2177)
                     /locus_tag="LRB02_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL4660301.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2192..2551)
                     /locus_tag="LRB02_002127"
     CDS             complement(2192..2551)
                     /locus_tag="LRB02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL4660302.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(2548..3399)
                     /locus_tag="LRB02_002128"
     CDS             complement(2548..3399)
                     /locus_tag="LRB02_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660303.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            complement(3414..4214)
                     /locus_tag="LRB02_002129"
     CDS             complement(3414..4214)
                     /locus_tag="LRB02_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL4660304.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(4257..5147)
                     /locus_tag="LRB02_002130"
     CDS             complement(4257..5147)
                     /locus_tag="LRB02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL4660305.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(5149..6090)
                     /locus_tag="LRB02_002131"
     CDS             complement(5149..6090)
                     /locus_tag="LRB02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL4660306.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            complement(6323..6658)
                     /locus_tag="LRB02_002132"
     CDS             complement(6323..6658)
                     /locus_tag="LRB02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660307.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            6925..7149
                     /locus_tag="LRB02_002133"
     CDS             6925..7149
                     /locus_tag="LRB02_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4660308.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(7146..7301)
                     /locus_tag="LRB02_002134"
     CDS             complement(7146..7301)
                     /locus_tag="LRB02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660309.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(7458..7613)
                     /locus_tag="LRB02_002135"
     CDS             complement(7458..7613)
                     /locus_tag="LRB02_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660310.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            7685..7915
                     /locus_tag="LRB02_002136"
     CDS             7685..7915
                     /locus_tag="LRB02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4660311.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            complement(7912..8070)
                     /locus_tag="LRB02_002137"
     CDS             complement(7912..8070)
                     /locus_tag="LRB02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660312.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(8083..8349)
                     /locus_tag="LRB02_002138"
     CDS             complement(8083..8349)
                     /locus_tag="LRB02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660313.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            8549..9253
                     /locus_tag="LRB02_002139"
     CDS             8549..9253
                     /locus_tag="LRB02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL4660314.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            9340..10629
                     /locus_tag="LRB02_002140"
     CDS             9340..10629
                     /locus_tag="LRB02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL4660315.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            10695..12047
                     /locus_tag="LRB02_002141"
     CDS             10695..12047
                     /locus_tag="LRB02_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4660316.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
BASE COUNT         3634 a         2343 c         1960 g         4123 t
ORIGIN      
        1 atttcctccc atttacgatc atcatttaat atcgaaatcc caaacttacg aatagcatca
       61 cttgcatcag caacaaactg acttgccact ttatatgttt cttctgctga aattccatat
      121 tctttttcaa actttgtctt tagtacattt agttcctgtt ttcttagttt tgctactctg
      181 cggtgtctgg tgttcattgt caatcaactc cctaatctga aagtgttgtc tatacttgat
      241 cgaaattctt ttaagtggtt ctctaccaca gaatcagtca cgttaaaacg atcaattaat
      301 actggagctg ccatatcttt caaataactt tgtctgatga ctaattcagt accatcagga
      361 agttctatgt taacctcccg accattgata attgcttgaa tgcccgcttc acttagtggt
      421 atttcgtatt tcatttcaca tcctccaaat cactcgactt cacgaataca ccatctacca
      481 ttttccctgt gcgtcctttg atttcgttgt atgccatttc taaacactct tgtacgtttg
      541 tccctttttg catggaaagg ataatcagcg tgacgataac gtctcctacg ctatctttaa
      601 atagttcatc attacttctt gccatcgccg aagctatttc cccgaattcc tcagctactt
      661 tcaaaaactg tgcttttgga tctgcttgat ccagtccctt gtctttagcc cactgctcta
      721 cttttgtgat tagttcgtcc attatttctc ctcctaaaat ttcatttcat cgttgttgtc
      781 atcttctttg tcatcagttt gggaaaggag cgcatacacg agatatgctg ctcctactaa
      841 acctaagaac aagagaattt tagccataaa gaatcccata ttatttgcct gcttcatccg
      901 taactacagt atctgctcca tttactgtta cccatccatg ctttttgcga gcttgagctt
      961 cttcataacg aatcaaattg tctgttactg attcggcgac tttgcgatta gattctgcct
     1021 ctgcttctgc agcttttgtt ttcttgtaag cttcactatc agcttgagtt tttgcagttt
     1081 ctgcgtctag cttcgctttt tcattttctt gaccagctcg aatgatcgca tcgattgatt
     1141 tttgtgtttc tttatcgaca tctggaacac caagtgttac gtcttcgact tcaaaccctt
     1201 tagattcaac tgatttagca aagttcgtta atacctcagc ttcaacttta gaggaatctc
     1261 ctgaaaggac atcaagcaga ctatatttcg cataaacttc acgagcgacc ttttgaagct
     1321 tagattttaa ccatccactt tcgatatctt ccgaagtgat attcccaaat tccttgtaca
     1381 tttttgctgc tttagttgaa tcaactttgt agtcatattt gatatcaatc gttgtctttt
     1441 tgccgtcgct tgtagatact gaaatatttt ttgattggat agtttgcaag cgaattggat
     1501 attggatcac tttgtcaatc cctacaaatt tcacaccttg cgtcagtgct tcatctttga
     1561 taccgccgtt cattgaatag cgcacaccca catatccatt atcgattttt tcgaagaact
     1621 taaatcctcc gataactcca atacctacga ttactgctcc tgctacaccc aatttgatta
     1681 atttgttttc gttcattttt cttcctccaa atacttatat tcgtgccctt ttcgatcggt
     1741 gtgatttcgc caagcgtacg ttcgaatagt cccacgcgta tatccagttt ctgcagacag
     1801 ctcactagct gttccttgca tcaaaatttt gccttcgtgt aaaacaacga cgattttccc
     1861 tttgcgctgt tttcttttgt ctggcttttt tcgtatacgt ccgttagcaa tctttcctag
     1921 acgctgaact tcagcaacca ctgcttcatc ttcttgccag ttctcatctt gaatcagcaa
     1981 cattaatttt cgccaagccg cttccttatc cacgctcatt cctccaatct acggatttcc
     2041 cttcttaaat tctctatgtg caaatcgatt gcctttctag ccgtttcatt gaccatcact
     2101 gcctttgttc gttccagatc gtcaatctca cgccgaaggc ttcgaattcg catttgaatc
     2161 acttcttctg ttgtcatgat ggaccacctc gttaaaaacg ctcttccttg aacgtattcc
     2221 gatatttttt ggctaatatc aacggcactt gatattgatg acagaacaac tttgccttga
     2281 tcttaaagtc ttttgtctgc atccccttaa catctacgac tttgaccagc ttgccgcttt
     2341 tgtaaaatgt gaagtcggga atatactcga tcttgcgata cttcttgccc tctagttcaa
     2401 acttcggcat cagctcaaac ctttcctgga gcttcacttt ccaaccattc gcttcagctt
     2461 gccacaaggc tagatcgtaa tactctgctt ccgcgataga atcaaacttg atacctcgat
     2521 gaacagtttt tttattacgg tatttattca tgcgatacta cctttcactg gttttatgcg
     2581 cttgtctgct gtttgagaaa ataccatcgt aaatccatca gagttgacaa acattcttga
     2641 tattgttctc gtaccgtaag cttttttcag ttcttcacca agcaagttcg ttgtgataat
     2701 cgttgctagg ttctgtcgtg catccaagaa cgaatttagc gtgtttatgc caaacgctcg
     2761 actatctgat gcatcttttc ctagttctga accgatatca tcaatgatga ctaaatccgc
     2821 tgttttgata tctgcaatca acgatccctc aatcgttttt ctgagttcag gattgttgta
     2881 cgaaaatttg atttgatcca gcatttcctg taacccgata aataagattt ttttatcgta
     2941 gtttgatcgc ttcaagactt cccaagccgc tgccatcgct aggtgacttt tacctgttcc
     3001 ttgttttcca gttaaaacaa ggtgacttgg atttcctaac aagactgaat taacaaagcg
     3061 tttggttact tcaaccgctt gtcttgtttc ttgatcgaca atttgataat tctgcaatgt
     3121 gcaatcaaac aacgcttgat taggcacaac agaaccacct ttgaaaaaat tgattgctct
     3181 tgctttcagg ctctcgttgt aaattcgttc tgtttgtaag tcttcctgaa ctcgcaatgc
     3241 tttataccca cattgcatac aggttggctt acaacgttct gagccatcag ggttcttcgt
     3301 acgccatcca tacaaaggct gtccgcattc aggacattcg ccacgttgga caagcacttt
     3361 ctgtatcagc ttttccatga tttccccaac agtttccatg cctacgcctc ctctcaaata
     3421 ggcaactcat ctgtgctaaa tttctcgtat tcaagcgttt tagtttgttt tgttttataa
     3481 tcacgatttg cttgcgctgc cattgtgtcg tatttttctc gcaatttttt agcagataaa
     3541 atattcgatg cccaaaacac attgtgctga ctccattcaa tcattccccg tacctggttt
     3601 tcggtccgct tgtcgatctc gatcattttc cgaatgttat ctgcccaact ttgcagattc
     3661 ggctttttga tttcctgatt ctgacaaatc tgtttgaata actcctccgc gagaatgtag
     3721 taaactgagt cggtgtcgta aacacgcttt ttgcgtggtt gcgacgatga tgttttatta
     3781 ttcttttcat tcttataatt cttttcattc ttgtttgtgt gcacttgttg ttcacttgtt
     3841 gtgcgtttga tgttcatttg ttgttcactg ctttggtaca aagaccagtt ttttattgat
     3901 ataacgctgt atttcgtagt tgatttgatg ttcaacattc cgtttttttc aaattgtttt
     3961 agccatctcc atacagaacc gctgttcact tgatgttcac gtttcacgcc ttcgttcatc
     4021 tcagatgtga tagcatcgcg ccctgtgacg aattctccgc tgttcacttg tatctctttt
     4081 ccattaaaaa gtatcttgcg attctcatga ctcgctttca tcaaacacaa gctccataat
     4141 ttatacatat aaggattggt ccaaacgaat gaattcatca cttttcgatg taatttgaca
     4201 tatcctgtgt tcattcgtat ttctcctcta gaaaagcaag ggagaaagct ccctcattat
     4261 ttgtttaacg gcggattaga tgcatcaaat aatccagttt gtacatcttc attttcttca
     4321 gatataacct ctgcttcttt tctttcagga atatcttctt caacttctgt ttcagcaata
     4381 atgctgccgt cttcttgaac cctttggact ctctcatccg atgtggtggc ttcttgcatt
     4441 tcgatggaca agattcccca tttagaaaga agatttctca aaacagtttt tcgtgccatt
     4501 gcattgtaat cagatgccca cacaccactt aactttgtct tatcgcgatc tttattgtta
     4561 gcaatccgat gagcttcgat ttcttgtttg gtccaataga cagttttttt gaatccattc
     4621 agtaactcga aatagccaac atatccaatg acttcatcag acgttctacc atttggatca
     4681 aactcaaact cttctgtcag tcggttccag ctttttagtt ctccttcgta aacttcgatc
     4741 acatttaatg ctttgtattt acctgatcgt tgggctaatt ggatatatcc tttatagcca
     4801 agcatgaatt gagctttctt ttcccatttt cctgtttgct tgtttttact attgaatgga
     4861 actaaatatg cataacctaa attcttatct agcccaagat ttaatgttgc agcagttaac
     4921 gcaccactca tgatagacat cggttcacta tctgcaagat aactgtcatt agatacaaga
     4981 gtcataacat tcgacataaa agcattagca ttgtcatgaa gtacttcttc aaatttcttt
     5041 ctcattgttg gtgtattcat tagagcttta agccctaact gtcctggtgc aacttgtttc
     5101 tgtggctttt ctgccaattg attttttaac gattcatttg ttgccatatt atttgatctc
     5161 cttttcggtt agccttcttg attcagtaac gttataaatc tcttcatcat ttgcgacatc
     5221 tggatatttc tctgctagtt tcttcgtgtt catccgtctt gtacgaacaa gtttccagct
     5281 gataatgttt ctttgtgtga taccgatact ggcttcacgt ttacctagct cactgatgat
     5341 ctcgttgtct acttgacgaa tagatgattc aatttctttt ttcgtccgct tgagttctct
     5401 tttttgctcg ataagttcat caaaacgtga tggtagagct gtttgatttt cttctacatc
     5461 tgcatatttt tcttttaaga agtcagcagt cgctccactt ccgtcaatta caggctcgat
     5521 acctccaaga acgttcgttt cccaaaactc tactaattgt tcagtgattg tatcgatcag
     5581 ctcttgatct cgttcaatcc gcttccaaat gaatttttgt ccaccgatca aaacagcgat
     5641 ataacaatag tctttgttta aaacgttcat gtaatgctga acttgacaga gatagctaag
     5701 cggtacctct tcaccttccc actcttttcc gagaaattga ttggctgttt tacattccag
     5761 aatggcattt tctcccacta cttcccgatc aatatttgct cttaaaaatg gatgtaatgg
     5821 atgttcaaat acttggtttc ttctgcgtac ttttttgcct gtacgttcct gaaactcttt
     5881 agcaaccact tcttctaaaa cattacccca ataagctggt tcgctttcta aatctttcaa
     5941 ttcaatttga cccgttttct caagccacaa ttgataagga gatttgtatt tgtttaatcc
     6001 aagtacggtt gcaacatctg agccaccaat acctcttttg cggtcttcga gccattcttg
     6061 atggctcatt tctaaggtag atttactcat cttcatcctc ctcatcaatt ggcgcttcat
     6121 atggtggttt agcataatca ggatcagtta aatagttgtc aagatttgct aactcgttca
     6181 tgtttaacct cttttctgct tgctaacacg caaagcacaa gcatggtaat tgcgaataac
     6241 aagccaaaga ttacataact tttcgctaca atcactaacg tgaaaaataa aataatgagt
     6301 agatcaattg ttttttcatt catcattgaa attccccctt gagaatacga gtcatcacat
     6361 cagctaaatc ttccttgtta ttaataacaa aagtatgatt tgaaggttga ttttcttctt
     6421 catttttcgc ttgttcgtaa gcatccacag caattctacg cataatatgt gctgacaatg
     6481 ggagattttc atctaaatgt tcttcttgca tagcaagaca aagtaaaatt tctgttgtct
     6541 caccataaac gagactttta agttcccctt ctttttgggc tgtacaaata atagaaacac
     6601 cttgcttttg gcattcacgt ttcaattctt cgataagatt ttcaattttc ttattcatgt
     6661 gataaaatct ccttaggtat aatttttggt ttgtgactca ttgctttgga cggctgagtc
     6721 acttttttat ttctaaccac ttcttctggc gttcacgcca ttgttcccct agcaatctag
     6781 gtttcatttc ctcgtggtat tcctgattta gttttcgcat caagtgttga tgctcttgta
     6841 atgtcatttg atccctcctt tcgtttattg aatttctcca tttctatttg ataaaatatt
     6901 tctatagaaa tggaggtgaa aactatggga aaaaatcaac atgttgtacc taattccaat
     6961 ggtggttgga atgtcaaagg tgctggaaat tctaaagcga ctgcacatac taatacaaaa
     7021 tcagaagcag taaaaatcgc tagagagatt tctaaaaatc aaggatctga gctatttatt
     7081 catggcagag atggaaaaat tcaaagccgt gatagtcacg gaaacgatcc acacccacca
     7141 aaaggttaat catatttagg agttaatcga atacgataac cgtcagcagg agttgcatcg
     7201 gtcaatgtaa cttctgctac tttttcccct tcttctgttt caataattaa tcttgtgtaa
     7261 ttgttctcat tcaaaatccc tatttttgat tcttccttca tgtcatccct ccttcgtctc
     7321 tcgccgtcac cacctccaag aagaggagtt gccaaaatga agaaacgtgg actttggcaa
     7381 acttgaaagg agagcagcaa ctcctctttt cggaagtggc gagtgtgtga tataatatta
     7441 ttgttctatg gtgcccacta cttgcttgcc ggctgtgtgg gcttttcttt ttgttcaatt
     7501 atggttgcca ccacagtaac tccccaatgt ctagaacgta ttctagccat ttcatctgct
     7561 aatctttgaa gattaggtgt gccattcaaa gtgatttttg gcttgttact cacaatctca
     7621 cctccatcag tttgttttct tttcacctct taatagaatt aacttgaaag cgaggtgaat
     7681 tgttatgggt aaaaatcaat gggtatctcc tagaaatggc aaatgggctg tacgtggtga
     7741 aggtaattcc gaagatacaa aactatttga taataaatca gatgctgttg agtatggtaa
     7801 aaagatttct aaaaaacagc aatcagagtt tattgtccaa aaaaagaatg gacaaataca
     7861 atcaaaagat agttttggaa acgatcctat acctccacgt gataaagaac attaatcata
     7921 tctaggggtc attcgaactc gaaaaccttc gcatgattca atatcattcg ccgtaataac
     7981 tgcgattgtt ttcgggttct gctcgtctgt ttcgacaaca atttttgtat attcttcaag
     8041 agtcggtact tttatattct tctcttccac tattcacacc tcctagctga cttgttttgt
     8101 ttcacctgtt attgatttcg gtttttccga atcagggtct aaaaaaatat cggaaactct
     8161 aacacccaat gccttagcaa ttttttctag agtggcgtac tttccgttcc tcattttatc
     8221 tacatcgttt tcgtaaataa atattgttct tgatgtcact ttgcttttcc ttgacagttc
     8281 ttcttttgaa taacccttta aagctcttaa ttgtctcaat gtatattttt ctttcttcgc
     8341 ggtatccata ttttattcgc ctcctctctt taactttctg actaaattat atacttcggt
     8401 ttttccgaag tcaagtgcat atttcagttt tcctgaaatc gatttctatt ttgcgtaaat
     8461 acttctttaa aaacactaaa atattttcta agaaacctgt tacatttttt ccgaaacggt
     8521 gttacaatta actcaaggag gtgttactat gttcgctgaa aatctaaagt ttcttagaga
     8581 aaagtataat attgatcaac aagaactagc cgaaaaacta ggaagaaaaa gttcatcctc
     8641 aataagcgaa tgggaaaaag gaaaatatac accaaaatta aaaaccttaa atgagatagc
     8701 taagatattc aatgttgata tagatgattt gatgaatatt gatttatcta tatctaatca
     8761 gatatataat atccttccta tatataatca gcttaacgac gaaaataaag ttgctaccta
     8821 tgaatttgca agtaatcgat tagaagaaca aaaacaagaa aatgtcctag attttcctaa
     8881 aaaagaaaaa atcccaactg ttcacaattc ggcttcggct gccaatccaa cagaacttac
     8941 ttacggtgat acggttgtcg aagaggaaga atttgagaga gttccaagtg ctgctgactt
     9001 cgcagtacct attataggcg attcaatgga acctgttatc aggaatggac aattcgtatt
     9061 cgttaaagaa caaccggacg tagaagatgg ggaaattgct attgtggaat taggtggtga
     9121 cggagttaca tgtaaagaga tatataaaga ttacgaaaat caaacaatca ttttacgttc
     9181 tataaacgac ttatacgaag atagaattgt atcgccagaa cagattagaa ttatcggaaa
     9241 agttgtattt taaaaaaatt cccatgccgg agccgtcaac ttcggtaagg gtttatatga
     9301 ttttgacaat tattttataa aagaagcgag gcgttttgaa tgggagtact attgttttta
     9361 gttggaatag ttttaataat tatgggtagt agcaaattat ctgaagctaa aaaattaaaa
     9421 gataaaactt ctgccttaga gagtaaggta aaggaatatt ctcaagaatt aaatgctagg
     9481 gaaacgttta tatctgaaaa agagaaaaac ataagtaaat tgaataaaaa cttgtcaacg
     9541 ctagaaaaca aaattgatat tcttaatgct gaaagccagc ggttagacga tgaaattaat
     9601 caaaaatatg acacatctct ggtcactatc tataaatcag taattattcc gaatatagat
     9661 gaaacttctt catccgaaat aaaaaacaaa ctttctttat taaagattaa agaaaaggat
     9721 tttattttag acggtgcaat taatattcct tcttatttga ataatagaga agctaataat
     9781 attaaaaaga aaatgcttat accattagaa tcagaaataa ctggacttct caataaactt
     9841 actatttcta acgtagattc aactagagaa aaaattataa gaacttttga taaaataaac
     9901 aagcttttta aaaacgatga agcagaattc aaaaaagaat tacttgaatt aaaacttcaa
     9961 gaattagaac tcaactattc atacatagtt aaagttaacg atgaaaaaga acagcaaaaa
    10021 gcaataaaag aacaaatgat tgaagaagaa aaggtaagac gtgaaataga acgtgaaaag
    10081 aaacgcattg ataaagaaga acgacagttt aactcagaga taactaagtt aatggcatac
    10141 atgcaaaaag ctagtgctga tgttgaaaaa gaactctatg ctgataaaat taaagaactg
    10201 gaagagaaat taagagaact agaaactgtc aaagaagatg tattgcaaag agaacttaat
    10261 actagagctg gctatgttta tgtcatcagt aatatcggat ctttcggtga agatgtctat
    10321 aaaataggaa tgactagacg gttagaacct atggatagaa ttaaggaact tagtagcgct
    10381 tctgtacctt ttgaatttga tgttcatgca atgatatttt cagaagatgc accaacatta
    10441 gaaaatcaac tacattcgca ttttagaaaa aacgaagtca ataaagttaa tcaacgaaaa
    10501 gaattcttta aagtatctct tgatgaaatt gaaaaagttg ttttagaaaa ttataacgga
    10561 actgtatctt ttactaaact agctcaagct gaacaataca gaagatcatt agaattaaca
    10621 gatgtgtagt aaaaagatta gccttcgggc ttttctttta caacgataag aacatacatt
    10681 cgaaaggaga taacatgaaa cgagtagcat tatatatgag agtatcaact gaacaacaag
    10741 ccaaacatgg agatagtctt agagaacaaa aagaaaccct ctatgaatat atcgaacaac
    10801 acaaggattt gaaagtagtt aatgaatatg tagacggtgg tatttctggt caaaaaataa
    10861 atcgtgatga atttcaaaaa ctattacaag atgtaaaaga aaataaaata gatctaattt
    10921 tatttacaaa attagataga tggtttagaa atctacgaca ttatttaaac actcaagaaa
    10981 ttttagaaaa acacaatgtt tcttggaatg ctgtttctca acaatattat gatacaacta
    11041 cagcgtatgg cagaactttc attgcacaag taatgagttt tgctgaattg gaagcccaaa
    11101 ttgattcaga acgcatcaaa gctgttatgg caaataaaat tgcacaaggt gaagtagtaa
    11161 gcgggaaaac tccgttaggc tactctatag aaaataagaa attagtaata aatgatgatg
    11221 ctcctatcgt gatagatata ttcaactact ttctttctag tggtagttta agaaaaactg
    11281 tttattattt aggctctcag tatggaatcg ttagagatta tcaaagtgtt aaaaatatgt
    11341 tgacgaacaa aaaatacatt ggtgaattaa gaaataataa aaattactgt cctcctatta
    11401 ttgataaaaa acttttttat gctgttcaaa aagccttacc taaaaattta aaaacaaatg
    11461 caaaacgtga ttacattttt aaaggtttat taaaatgttc tgactgtcag ggatcagtag
    11521 ctggtcaaac tattaaagca agatataaga aaaaagacgg tacagaatct atatacgaaa
    11581 ggacttgtta tagatgtgta aaacgaagaa ataataaatt acgttgcaca aataagcgcg
    11641 ctttttacga aaaaaattta gaacgttata tttttgaagc tactaaacaa aaatttgaac
    11701 aaatacaaat aaactattca aaaaaacaac caaagattat taaaaagaaa aatagtaaga
    11761 aaaatattga gaataaatta gatagattga agaaagccta cttgaatgaa gttatagact
    11821 tagaggaata caaaaaagat cgagaagctt taatgaaaga attaaatgaa attgaagtag
    11881 agccagctaa aatagatata aaaaatgtcg aatttattct atcaaaagaa ttcgatgaaa
    11941 tttataaaga atcttcggaa gaagaaaaaa atgcattatg gcgttcaatt atcgataaca
    12001 taatagtatt tccagatggc aatataacag taaatttcct tatataattt atgtgtacta
//
LOCUS       DAFFQY010000080        11865 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFFQY010000080 DAFFQY010000000
VERSION     DAFFQY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.080"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..637)
                     /locus_tag="LRB02_002142"
     CDS             complement(<1..637)
                     /locus_tag="LRB02_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL4660317.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQ"
     misc_feature    823..899
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            919..1227
                     /gene="rplU"
                     /locus_tag="LRB02_002143"
     CDS             919..1227
                     /gene="rplU"
                     /locus_tag="LRB02_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL4660318.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1254..1598
                     /locus_tag="LRB02_002144"
     CDS             1254..1598
                     /locus_tag="LRB02_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL4660319.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            1611..1904
                     /gene="rpmA"
                     /locus_tag="LRB02_002145"
     CDS             1611..1904
                     /gene="rpmA"
                     /locus_tag="LRB02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL4660320.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            2338..5145
                     /locus_tag="LRB02_002146"
     CDS             2338..5145
                     /locus_tag="LRB02_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4660321.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            5336..5827
                     /locus_tag="LRB02_002147"
     CDS             5336..5827
                     /locus_tag="LRB02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4660322.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5930..6919
                     /gene="manX"
                     /locus_tag="LRB02_002148"
     CDS             5930..6919
                     /gene="manX"
                     /locus_tag="LRB02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL4660323.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6952..7755
                     /locus_tag="LRB02_002149"
     CDS             6952..7755
                     /locus_tag="LRB02_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL4660324.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7776..8690
                     /locus_tag="LRB02_002150"
     CDS             7776..8690
                     /locus_tag="LRB02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL4660325.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8773..9147
                     /locus_tag="LRB02_002151"
     CDS             8773..9147
                     /locus_tag="LRB02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL4660326.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            9286..9531
                     /locus_tag="LRB02_002152"
     CDS             9286..9531
                     /locus_tag="LRB02_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4660327.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            9703..10767
                     /locus_tag="LRB02_002153"
     CDS             9703..10767
                     /locus_tag="LRB02_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL4660328.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            11013..11438
                     /locus_tag="LRB02_002154"
     CDS             11013..11438
                     /locus_tag="LRB02_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4660329.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            11431..>11865
                     /gene="nusB"
                     /locus_tag="LRB02_002155"
     CDS             11431..>11865
                     /gene="nusB"
                     /locus_tag="LRB02_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL4660330.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT         3827 a         1972 c         2621 g         3445 t
ORIGIN      
        1 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
       61 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
      121 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
      181 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
      241 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
      301 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
      361 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
      421 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
      481 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
      541 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
      601 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
      661 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
      721 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
      781 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
      841 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
      901 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
      961 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     1021 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     1081 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     1141 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     1201 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     1261 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     1321 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     1381 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     1441 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     1501 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     1561 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     1621 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     1681 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     1741 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     1801 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     1861 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     1921 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     1981 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     2041 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     2101 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     2161 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     2221 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     2281 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     2341 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     2401 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     2461 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     2521 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     2581 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     2641 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     2701 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     2761 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     2821 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     2881 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     2941 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     3001 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     3061 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     3121 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     3181 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     3241 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     3301 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     3361 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     3421 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     3481 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     3541 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     3601 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     3661 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     3721 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     3781 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     3841 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     3901 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     3961 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     4021 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     4081 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     4141 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     4201 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     4261 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     4321 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     4381 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     4441 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     4501 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     4561 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     4621 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     4681 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     4741 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     4801 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     4861 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     4921 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     4981 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     5041 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     5101 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     5161 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     5221 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     5281 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     5341 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     5401 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     5461 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     5521 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     5581 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     5641 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     5701 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     5761 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     5821 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     5881 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     5941 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     6001 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
     6061 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
     6121 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
     6181 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
     6241 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
     6301 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
     6361 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
     6421 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
     6481 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
     6541 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
     6601 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
     6661 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
     6721 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
     6781 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
     6841 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
     6901 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     6961 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     7021 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     7081 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     7141 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     7201 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     7261 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     7321 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     7381 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     7441 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     7501 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     7561 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     7621 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     7681 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     7741 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     7801 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
     7861 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
     7921 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
     7981 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
     8041 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
     8101 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
     8161 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
     8221 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
     8281 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
     8341 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
     8401 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
     8461 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
     8521 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
     8581 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
     8641 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
     8701 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
     8761 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
     8821 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
     8881 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
     8941 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
     9001 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
     9061 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
     9121 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
     9181 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
     9241 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
     9301 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
     9361 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
     9421 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
     9481 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
     9541 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
     9601 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
     9661 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
     9721 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
     9781 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
     9841 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
     9901 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
     9961 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    10021 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    10081 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    10141 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    10201 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    10261 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    10321 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    10381 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    10441 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    10501 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    10561 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    10621 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    10681 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    10741 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    10801 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    10861 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    10921 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    10981 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    11041 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    11101 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    11161 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    11221 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    11281 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    11341 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    11401 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    11461 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    11521 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    11581 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    11641 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    11701 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    11761 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    11821 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DAFFQY010000081        11860 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFFQY010000081 DAFFQY010000000
VERSION     DAFFQY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.081"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LRB02_002156"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LRB02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL4660331.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LRB02_002157"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LRB02_002157"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL4660332.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LRB02_002158"
     CDS             complement(2169..2906)
                     /locus_tag="LRB02_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL4660333.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LRB02_002159"
     CDS             complement(2997..3524)
                     /locus_tag="LRB02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660334.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LRB02_002160"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LRB02_002160"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL4660335.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LRB02_002161"
     CDS             complement(4401..4886)
                     /locus_tag="LRB02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL4660336.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LRB02_002162"
     CDS             complement(5039..5236)
                     /locus_tag="LRB02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660337.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LRB02_002163"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LRB02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL4660338.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LRB02_002164"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LRB02_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL4660339.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LRB02_002165"
     CDS             9211..9423
                     /locus_tag="LRB02_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL4660340.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LRB02_002166"
     CDS             9425..11104
                     /locus_tag="LRB02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL4660341.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LRB02_002167"
     CDS             complement(11358..11558)
                     /locus_tag="LRB02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4660342.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11860)
                     /locus_tag="LRB02_002168"
     CDS             complement(11708..>11860)
                     /locus_tag="LRB02_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBL4660343.1"
                     /translation="GNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREE
                     GMMEEK"
BASE COUNT         3428 a         2567 c         2064 g         3801 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc
//
LOCUS       DAFFQY010000082        11603 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFFQY010000082 DAFFQY010000000
VERSION     DAFFQY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.082"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            108..971
                     /locus_tag="LRB02_002169"
     CDS             108..971
                     /locus_tag="LRB02_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4660344.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1100..2722)
                     /locus_tag="LRB02_002170"
     CDS             complement(1100..2722)
                     /locus_tag="LRB02_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4660345.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2971..3492
                     /locus_tag="LRB02_002171"
     CDS             2971..3492
                     /locus_tag="LRB02_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL4660346.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="LRB02_002172"
     CDS             complement(3678..5408)
                     /gene="spxB"
                     /locus_tag="LRB02_002172"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL4660347.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5573..6391
                     /locus_tag="LRB02_002173"
     CDS             5573..6391
                     /locus_tag="LRB02_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL4660348.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6494..6691)
                     /locus_tag="LRB02_002174"
     CDS             complement(6494..6691)
                     /locus_tag="LRB02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660349.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6860..7438
                     /locus_tag="LRB02_002175"
     CDS             6860..7438
                     /locus_tag="LRB02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL4660350.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7533..7670
                     /locus_tag="LRB02_002176"
     CDS             7533..7670
                     /locus_tag="LRB02_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660351.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8483..8986)
                     /locus_tag="LRB02_002177"
     CDS             complement(8483..8986)
                     /locus_tag="LRB02_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4660352.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="LRB02_002178"
     CDS             9344..10369
                     /locus_tag="LRB02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4660353.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="LRB02_002179"
     CDS             10692..11570
                     /locus_tag="LRB02_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL4660354.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3595 a         1974 c         2261 g         3773 t
ORIGIN      
        1 attttgatta gaacttgcga aaataaaccg gttaaagtgt gtctagcgca agagatttgc
       61 tataatagac gataagggta aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg
      121 taacagattc ttgttgtgat ctgccatacc atgtattagc aaaagaaaaa gttgattttt
      181 tatcaatgaa tctccaattg aatgatcaag agttagtaga tgacctcgga aagacattca
      241 attatgaccg ctacatgaat cagctgaaaa atggtgcgat gcctacgaca tcacaaatca
      301 atgttggccg atatttagaa tttttccgtt cttatgcgga aaaacagatt cctgttttat
      361 atgtggcttt ttccagtgga ttaagcggct cttacagtag tgcgcttcaa gcggttgaat
      421 tgctgaagga agaatatgaa gaccctgaga tctatgtttt tgatacaaaa gctgcaagta
      481 ttggggaagg gcttttagta ttggaagctg taagattgaa aaaagaaggt aaaagcatcg
      541 aagaaaccct agagtggctg gaaagtaatt atttgaaagt ccattcatgg gtaacagtag
      601 acgatctgaa atatctagaa cgaggcggta ggatttccaa agcaagtgct actcttggca
      661 gtctattgag tgtaaagcct attattacgg tggataaaga aggaaggctg acgaatgttg
      721 acaaagttcg aggaagaaac aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa
      781 tcgttgcacc acttgatcaa atcgtttatg tggcttacgc tggggatcta gagagcgcgg
      841 agaaggcaaa acggcttttg gaagagcaga taaagatgaa agatgtgaag ctttatccct
      901 taggaccaac gatcgccagt cacacaggct atggctgtat cgcgattttt tcgatgggaa
      961 tcagtcgata aaagcagtaa ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa
     1021 acagcgactc ttaaaagata gatgagcatc ctatctctta gaggtcgctg tttttttatt
     1081 ttttaagcta aataggccat tatgcccata actcgttcaa ttgtttttga atatctgcat
     1141 tttccaaaaa ttcatcatat gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg
     1201 caatgatgcg gtttgccaat gtttggataa attgatggtc atgtgacgca aataagattg
     1261 agccagtaaa tgccatcaag ccgtcattca atgctgtgat tgattctaag tctaaatggt
     1321 tcgttggatc atctaataca agtacgttag ctttcgaaag catcattttt gaaagcatgc
     1381 aacggacttt ctctccacca gataaaacat tcactggttt caatacttct tcaccagaaa
     1441 acaacattct tcctaagaag cttcttaaga aggtgttatc atcttcttct ttacttgcat
     1501 attgacgcaa ccagtctaaa atcgttagat cagtatcgaa ttctttcgtt gtatcctttg
     1561 gtaaataaga attacttgtt gtcacacccc aacgtactgt tccagtatct ggcgtaattt
     1621 cacccatgat aactttgaat agcgttgttg ttgttatatc attttctgcg acgaaagcga
     1681 ccttgtcttc tttgctcaaa ttaaaagata tatcatctaa aattttcttt ccatcaatcg
     1741 ttacacttac attttctacc tgcaataagt cgtttccaat ctcacgttca ggtttgaatc
     1801 caacgaatgg ataacgtcta gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca
     1861 tttttttacg tgaagttgct tgttttgatt ttgatgcatt cgcactaaag cgggcaataa
     1921 attcttggag ttctttgatt tgttcttctt ttttggcatt tgcgtttgct tgaagtttag
     1981 ccgctagttg actagattcc aaccaaaaat cataatttcc tacataaagt ttgattttac
     2041 caaaatctag atccgccata tgtgtacata ctttgttcaa aaagtgacgg tcatgggata
     2101 caacgatcac agtattttca aaattgatca aaaattcttc cagccaattg atcgattgga
     2161 tatccagacc gtttgttggt tcgtctagca aaagaacatc aggtttacca aatagtgttt
     2221 gtgctaataa cacttttacc ttttgtccac cagttaattc actcatttgc tgatggtgca
     2281 attcttcagg gatgttcaga ccttgcagta aaacagctgc ttctggttca gcttcccatc
     2341 cattaagctc agcaaattct ccttctaatt cagctgctcg aataccatct tcgtcagtga
     2401 aatcctcttt catgtaaatc gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc
     2461 ccatgatgac tgtttcaagt acagtatagt cttcatagtc gtaatggttc tgtttcaata
     2521 cggccatccg ttcatctggt cccatagtaa cggaacctgt ggaaggctgc agatcccctg
     2581 ataacacttt aaggaacgtt gatttacctg caccatttgc accaatcaag ccatagcagt
     2641 ttccgggggt aaatttaata ttcacatcat caaatagttt gcggtctgaa aagtgcaaac
     2701 tcacatcatt tacagtaatc aatttttttc ctcactttta tcattattag ttttgacaag
     2761 tcttatagaa ttatataggg gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt
     2821 ttcacgtgaa acagaatgaa aaaaataaaa ataaaagcgg agcgaaaata gctactatag
     2881 gatttattag tttgcaacag cataattttt gacgttattt ataagaggga gtactataaa
     2941 actgtaaaag aaatgaaaga aggattgaaa atggatgaaa aaagaaccat cgactggggc
     3001 tcattgattt tgggaatttt atttgtactt gtctcattgt tatcatttca agaccctgtc
     3061 ggaaatttag ttgcaattgt agtagtcttt gcgatttttg cctttattaa agggatattt
     3121 gaactgtttg tacggaacag attaaaagag ctaacaggat ataaaggaaa gatgccctta
     3181 ataatcggga tcatcgatat tctagttggc gtctttttct tattcaatat tggtgcagga
     3241 gtcgcagcat tgccttttgt ttttgcagta tggtttattg ctgattcagt tttagcgtta
     3301 tttactgctg acttggcaag aggagtaagt gaaggatatt attggtttac gatcatcgtc
     3361 aatattttag gaattttatt aggaattttc ttattgttta atccaatttc ttctgcgttg
     3421 acattaagct ttttagttgg attctatttt atgttatttg gtatcacaca tatcgtctat
     3481 gcatttagat aagtaaaatc aaacaaataa aaaagccggc ttccgattac agttttactg
     3541 ttgtaattgg aagccggctt ttttatcatg gacaaatatg cacgaggtga tttttaggtg
     3601 tcgagacaaa tttatgcaaa aatgtgagaa gctatataca caaattttat cttaatactc
     3661 gaagcatagc gcagttgtca caacctcttt tcatataatt cattcaatac tggcaaatca
     3721 tggacttcat atttttcttt gaatgcttgg atttcttctt cagaatattt tcttggatct
     3781 aaatgcagat cttctactgg caatgggcgc ttgttattga tcttcacatc gattactaca
     3841 ggacgatctg attttgcagc tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact
     3901 gtaaagctat ttgccccgag cgcttctcca actttaccga aatctgcatc ttctaaaaat
     3961 acaccaaatt tcttttgttc cgtatcttct tgttctgctt cgatgaaacc aaaagaatca
     4021 ttcgaaaaga caacattaat aattggaagc tgatatttga cttgtgtaat gatatcttgc
     4081 atgaccatag caaacccgcc atcaccactt aaggtaaata cttgtttttc tgggtagctt
     4141 agttgtgcgg caattcctcc tggcacacca ttacccatag ttgcgaacca tccagatgtc
     4201 gtatgttttt gtttaccgtt catattcaat aaacgaatag aatggatagt cgtgttaccg
     4261 acatctgtga cgaaaatcgc atcgtcttca gcaatacgat tgatttcttt atagacagcc
     4321 tctggacgaa tcggttcttc ctctcgatct tcaaaagatt tcaaccaatt tacccatttt
     4381 tctttatttt cttgatttgc taggtaccag ctatcagcag ggcgagcatc ccctaactcg
     4441 acaagtcttt gcaaagctgt gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt
     4501 cgtccaaatt ttgacgcatc gatatcgatt tggataaatt cagcagttgg attaaaaaat
     4561 gcacgtccaa atgggaagtc gctaccgaca aataagatca gatcggcttc tgccaatgct
     4621 tcatttccgg gtttagtagc gacacgtcca gcaaaaccaa gaaagttttc ataatcatca
     4681 ggaataattc cttttgctag aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat
     4741 tctttgattt gcggaaatcc gtttcgcgtt ccttgaccaa cataaagaac tggttttttt
     4801 gctttttcaa gataaggcaa agcagctagc aaatcttttt cttctggtaa tatcacactt
     4861 gttttgtgag tgtgagcggt ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca
     4921 acagggatag tcacgatagc cacgccttta tgctcatagg cggctttaat ggcttcatct
     4981 actacatgcg gaagactttg tggtgtcatg actgtacgat tgtatacgct aacatctgcg
     5041 aaaataggat tttcatttag ttcctggaaa gagttatagt tcatggatgt agaagcaact
     5101 tgtcctaaga acgctactag aggcacatgg tccatctgtg catcatacaa accattgatc
     5161 aaatgtgtcg caccaggtcc tgcagagccg aagacagcgc cgactttccc cgttaatttt
     5221 gcatctgcag cggctgcgag tgcgccgact tcttcatgac gaacttgaat gtagttgatt
     5281 tcggtttttt cttgatatag cgcatccata attgaattga aagatcctcc gggaatgccg
     5341 taaatatggt cgatacccca gctttcaaga acttttacca ttgcgattcc tgcattgatt
     5401 tttgacatgg tgtttcactc ctttagcaat gatgatacat aaattttttg tatctagagt
     5461 atagaacttt ttatcgaaaa tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt
     5521 tttgggaata tgctagagta gtacatagat agattcaaga gaggggaatg gcatggttga
     5581 atggctattg agacttgaac cttggcagca agcacttgct gggacagctt ttacttattt
     5641 tatgacagct cttggtgccg ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct
     5701 aaacttgatg ttaggatttg cttctggtgt catgattgct gcaagttttt ggtctttgtt
     5761 agatccggct attacaaaag cagaagaaaa tggagatatt gcttggctag ttgtaagtat
     5821 tgggtttggt ttaggcggtg tgtttttata tatggcagat aaaacactgc cccatatgca
     5881 ttttggacca caacatgaag cagaaggatt gcctacccat ttaaaacgga cgatcttgct
     5941 tgttttttct attacattgc ataacattcc tgaaggttta gccgtggggg tagcattcgg
     6001 tgcagcagca actgcggata atccgacagc cgctatatta gccgctgttt cggtggcgct
     6061 aggtattggt atccaaaact tcccagaagg agcagctgta tcaatcccgt tgcgacaaga
     6121 aggtctgagt cgtaaaaagg cctttgttta cggacaagct tctggaatcg ttgaaccaat
     6181 tgctggagtg attggagcgc tactagtaac gaaagtggag ctgctgcttc cttatgcact
     6241 tgcttttgcc gcaggagcaa tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca
     6301 gcagacattg tccagcaaac gtcattttgc agtttttggc gtcatgagtg gatttatcat
     6361 tatgatgatt ttggatgtgg cattaggata aataaaaaaa atatatatga tatatcaaat
     6421 agggccgcga acttatatcg cgaccctatt tttttagtaa gttatatcat aatgaagtga
     6481 atggtatcat acattaataa tagttttttc tatgtgtatt tcctttgatc cagcgaacaa
     6541 gccagatgat aagtgcgatt gggattagaa ctttgaccgc aaaccataaa agcccgctta
     6601 ggacactgac aaaaagactg atgacgaagc tagctgcaat tagcactaaa agaaccatga
     6661 agatatttaa gaacatattt ttttgacgca tacaaaaccc tcctcttgtt cattctttac
     6721 tatagaaaaa gtataacagg gattgtttaa aaacacctcc aactgaagac tgatttttac
     6781 ttcatctact taaatgatca aacaattgct ttttctctgg tttatcatct aaaatagagc
     6841 ggaaaaggag gagaatatca tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat
     6901 cgcgattatc atgactattt tagttttaga acttcatgaa cctaacggag acacgtggga
     6961 ggcgctcgct gcattaggtt accgattttt tatctatatt gtcagtttta tcattttagc
     7021 gatttattgg aacaatcatc atcatttatt tcaagttgta cataagataa atggacgggt
     7081 cttatgggcg aacagtttct ttgtttttgc gctttcgctt tttccgtttg caacttcatg
     7141 gatcagtgag tacatcaata gcctggctcc agaaatgact tatggttttg ttaccttgac
     7201 cgctaatatt gcgtattata tcttggcaaa agaacttgtc aaagcaaata aaggccacga
     7261 gaaagcattg aaggcgatgt tgaataatta caagaaatcg tatctatcga ttggtttgaa
     7321 tgtgttagga attgtcttag ggtatttagt agcgccagta gccgtgctga tcgcaaacat
     7381 cgtgattcta gctgcttgga tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa
     7441 tgatccgtgt ttaaaattta agagaaaaat atagaaaaaa taaataaacg atcaaatatc
     7501 cgagtgatac tagtattcaa agagggatgc agatggatca aaaaagaatt catgtaggac
     7561 tgattactag ttttgtcacg atgcttattt atttggatca aggtcatttg acttggacag
     7621 tagttggtgt cgtaggattt ttgacattat tcacagcaat aaaaaaataa acatctttat
     7681 gtaatctgat gttctatcta tttaagaaaa gcatgtttac agcagtatga ttctgttgta
     7741 aacgtgtttt tatttgtgga aaatttaatt tttagaaaag tgtatcgaaa aatagatagt
     7801 tagtttttcc acagtggata acttcgtaag agataaaaat aaaaaattag tcaaagaagg
     7861 gcgtatagat ttcttttgat aggagatata caacagaaat gaataaatat taacgtgttt
     7921 tcaaacagta taaaacggtg tggtttttgg aataaaaaaa taacatatct cattttacac
     7981 ttttgttatt ttatacttaa aaaataacga cacagaaaat accgtgtctc agatttctct
     8041 actacttcta aaaactactg tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc
     8101 agaatatact ggcagaatca aggagaaggg agcctaaata aggagaaaaa ccatagaaaa
     8161 tataaagtaa ttacttataa aacaagcttt ttatagaaaa ataatatacc tgagtagggt
     8221 ataaattaag acggaattcc tttctagatt gagatattga ctaggaagaa tgttaagatt
     8281 ttaagccttt aagctaaaaa aaaacccttt ttgttaaata aggggaatat agcctgtcag
     8341 tttcaaactg caagtcgtcc tgctgcaata atatagtaga ttagagatga aaatagattt
     8401 ttttaaacca atgactgaat aaaaagaccg tcatagatac acattggaat gtgtctatta
     8461 cggtcttttt ttacgaacga ttttattgaa gacggacagt tcgggtcaaa aataaaaaag
     8521 ctacgatcag aaataagaat aaaatgtatc ccaaaataat ggctagacgg tctaacttat
     8581 ttgtcgatag atgtttgttt agaagttgat aggcccataa acctatcagt cctcctgttg
     8641 tattagttat caaatcagtg atgtccgcta cgccaatacc gaataggaat tgaatcaatt
     8701 ctgccaagaa gcttacaagg aaaataatcc ctaatttttt agaaaaagaa agtctcttga
     8761 aattgacatt caaaagcacc ccaaaaggaa aaaagacaat caagttataa gtggtctcac
     8821 gtaataccgt cgtattttca aaagaaaaag tagaaaaagg gacgagattg acattgctgt
     8881 aagaataagt gagaaattgt ggaagattaa atgatagctt gaataaaatc aaccataata
     8941 gaaagaataa ataaatccct aataaaaatt ttgatacata ccgcatagac atctcctcca
     9001 tttaaaaagt ataccatcga aaaccgctga aaaatcataa aaaagtatga agtttaagga
     9061 ccaacgtagt tatcgtacga aaattagaag ctttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt ttaaaagagc gtg
//
LOCUS       DAFFQY010000083        11423 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFFQY010000083 DAFFQY010000000
VERSION     DAFFQY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.083"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..551)
                     /locus_tag="LRB02_002180"
     CDS             complement(<1..551)
                     /locus_tag="LRB02_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL4660355.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
     gene            complement(548..967)
                     /locus_tag="LRB02_002181"
     CDS             complement(548..967)
                     /locus_tag="LRB02_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBL4660356.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(964..1182)
                     /locus_tag="LRB02_002182"
     CDS             complement(964..1182)
                     /locus_tag="LRB02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660357.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(1169..1525)
                     /locus_tag="LRB02_002183"
     CDS             complement(1169..1525)
                     /locus_tag="LRB02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL4660358.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(1525..1830)
                     /locus_tag="LRB02_002184"
     CDS             complement(1525..1830)
                     /locus_tag="LRB02_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660359.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(1827..1988)
                     /locus_tag="LRB02_002185"
     CDS             complement(1827..1988)
                     /locus_tag="LRB02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL4660360.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(1985..2287)
                     /locus_tag="LRB02_002186"
     CDS             complement(1985..2287)
                     /locus_tag="LRB02_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660361.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(2280..2444)
                     /locus_tag="LRB02_002187"
     CDS             complement(2280..2444)
                     /locus_tag="LRB02_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660362.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2449..2718)
                     /locus_tag="LRB02_002188"
     CDS             complement(2449..2718)
                     /locus_tag="LRB02_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660363.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(2730..3479)
                     /locus_tag="LRB02_002189"
     CDS             complement(2730..3479)
                     /locus_tag="LRB02_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660364.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3482..4168)
                     /locus_tag="LRB02_002190"
     CDS             complement(3482..4168)
                     /locus_tag="LRB02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660365.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(4174..4845)
                     /locus_tag="LRB02_002191"
     CDS             complement(4174..4845)
                     /locus_tag="LRB02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL4660366.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4838..5179)
                     /locus_tag="LRB02_002192"
     CDS             complement(4838..5179)
                     /locus_tag="LRB02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660367.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5357..6055)
                     /locus_tag="LRB02_002193"
     CDS             complement(5357..6055)
                     /locus_tag="LRB02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL4660368.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6142..6612
                     /locus_tag="LRB02_002194"
     CDS             6142..6612
                     /locus_tag="LRB02_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660369.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6655..6834)
                     /locus_tag="LRB02_002195"
     CDS             complement(6655..6834)
                     /locus_tag="LRB02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660370.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6821..7159)
                     /locus_tag="LRB02_002196"
     CDS             complement(6821..7159)
                     /locus_tag="LRB02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL4660371.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7175..7378)
                     /locus_tag="LRB02_002197"
     CDS             complement(7175..7378)
                     /locus_tag="LRB02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660372.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7393..8169)
                     /locus_tag="LRB02_002198"
     CDS             complement(7393..8169)
                     /locus_tag="LRB02_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL4660373.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8468..8788
                     /locus_tag="LRB02_002199"
     CDS             8468..8788
                     /locus_tag="LRB02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660374.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8806..9237
                     /locus_tag="LRB02_002200"
     CDS             8806..9237
                     /locus_tag="LRB02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL4660375.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9367..9828
                     /locus_tag="LRB02_002201"
     CDS             9367..9828
                     /locus_tag="LRB02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL4660376.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            9862..10497
                     /locus_tag="LRB02_002202"
     CDS             9862..10497
                     /locus_tag="LRB02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL4660377.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            10610..11245
                     /locus_tag="LRB02_002203"
     CDS             10610..11245
                     /locus_tag="LRB02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660378.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
BASE COUNT         3372 a         2206 c         1770 g         4075 t
ORIGIN      
        1 tctaccacct cttccactgt tccattcacc aacaatgctg cagcatccgc tgttgcttta
       61 tcagtgaaaa gcaatcgatg agatggattg cattcaaaac tataatttac tccatctttt
      121 ccccataaag cacatttagt aagatagttt ccatcggcat tctttattgc ccataacggc
      181 tctttctcaa cctcgtagcc gttgtatagg ctcaatagtg tttcatatga ttgtcttgtt
      241 gcccaagcga gtaattcttc tccttgctct tgcgtcacta tgccatctcg tacaaaccaa
      301 tcacagaaat aatatgaatc accatcttta gatttgatta aatacgctat tttttcagct
      361 tttgtaaaag ggtccgtaga tttctcaagc caaccggcca cgagttgtgg aataactggt
      421 ttctgcggtt cgtctatttt tttcattaac gtaatacctg aatttatact ctcgttatag
      481 caagcagcta ttgcatcatt tcctatcgct ttaatacttt ctaacttttt gattgcttcc
      541 tgtttattca tcgctgttcc tccaataact cgctattctc gtatatattt ccggtgactt
      601 cgtacgtgta ttcttcaaac agctctgtgt taaaaatttg atactctaag tcttcatcaa
      661 cagtggcaca aactaatccg gaataatcta ttgaattttt gacaatacag actttattat
      721 ctaagtaatc gaagccattt cgcacgctga ctaatactat atccccttca aatatatcca
      781 caccattctt atctttcatt cctgtggatt gcatgaggtc gatgtttcgc acgtgatatg
      841 atttcaattc ggtaggattt atccaatatt caataaagtt tgttttgccg tttttagtaa
      901 aatgcaagac ggctacatct ctcattacgt tctttcgttt atcccacgct ctaaacttcg
      961 gtatcattcg ctgtcctcct cgtatttttc aatcaattcc attacttttt tcactatttc
     1021 aaactcaacc gcttttgatt cttcgaaatc atgaacaatt tctggaaaca gtacatcatc
     1081 aactacccac aaaataatct ggcgctttcc accaaaattt atgattagat ggtccgattc
     1141 cacagatagt gttgctcctg attcgatatc atataaatcc atgctgaatt gaacgagttt
     1201 ttttatcatt tgctgtcctc ctcaaatact cttctaatat ttctttatac ttctctacaa
     1261 atttgaaacg atcttgatga agtttcttgc tccaatttgt ttgccgatcc agctcacgca
     1321 tctgatcgaa ccctttttga atttcgttgt aataaaattc aatgtttgct gctgctttcc
     1381 aatgcctgct acttcgcact cctgctcctg tttcagccat ttctaactta actaattcag
     1441 ctcgttcttt tgatttttta tctttctgaa tcttcatcat gattttcttg aggatgatgt
     1501 cactgtattg tgtaatgaga tccattatct ctcctccaca tacctaaact gtcgtccttt
     1561 tgaatcaatc cataagctcc tagctctatc ccagataatg tttttgctta atccagtaat
     1621 ttcagataac tgttcagcag tacctgttac taaaattcgg tcatcatgcc agattgcaat
     1681 ttttctcggc gttctccgtt tgggcttttc agtccacatt gatttaccga gcttttggac
     1741 ttctgcaact atttctttgt cttcttgcca agattctgac ttggttaatt cagcaattct
     1801 tttcattgct gctttcttat ccacgctcat tcctccaatc gatggatttc ccttcttaaa
     1861 ttctctatgt gcaaatcgat tgcctttctc gccgtttcat tgaccatcac tgcctttgtt
     1921 cgttccagat cgtcaatctc acgctgaagg cttcgaatac gcatttgaat cacttcttct
     1981 gttgtcatga tgattcctcc acgtatctaa acgttctctt cttagcgtct gtgtagccac
     2041 accttgctct tttcctcacg attttctcgt gcaagcctgt gagacttgct aactgttcag
     2101 ctgtccctat cactagaagt ttgtcaccgt gccaaattgc gatttttcgt ggtcttggct
     2161 tgttgctctt gtctgcccac atcgctcttc caagcctcat cacttctgaa gcagcttctt
     2221 tgtcattttg ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct
     2281 tatccatccc gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt
     2341 ccgccatgga acgtgttgag ccacttgtct tcgtacgata cactttgtct taaagcttct
     2401 tgatgcatta gttcaatctg tgctgtaaat gtctttaggt ccatctgatt acacctgctc
     2461 aagttcatta agatgttttt gcaagccttt aacacaatca acaaatagta atttggtata
     2521 tgctaaattt cttaattgtg ttgtatcgat ataaagtgcg aaatagtatc tgagcttact
     2581 ccaactcgaa cgatcattct taattccttc aattccagct tcttcgagtt gatcatatac
     2641 gtctctcagg acgtctaatt cctcgccctt tttataattc gctatttcat taattagttc
     2701 tagataatcg atcttcattt ttccacctct tagaatggtg cttttgattg tctattagct
     2761 cgttctagcg cttttttctt gagataggct tcttggtcga ttgcccattc aggaagcttc
     2821 tctcgtcttc ctgtgcgctt gtatccactg cttgcgttct taggttcact tttttctttc
     2881 cttgcccaac ttcgaatagt tgccaaatag tttttataag tcttaccaga tgattcacaa
     2941 tactctgaca gtcgttcgat tcgctcttgg taatcattag ggaattctgt tttgagtttc
     3001 tccatctgct catctgacaa aagaacattt ttatactctc cgtatttatg acggacgggc
     3061 ttagccttcg attttttcga aggcggtaac tctcttatat attcttttgt attattaaat
     3121 gtattattaa tagatgtatt attatctttg actttttcgt caataggggt attgcgtttt
     3181 tcgtcaatag ggtattgatt aattcgtagg tacctattga ttatttgatt ggtaccctct
     3241 ttgtaaatga tttcccgatt caagtatcca aacttaatca aatcacttac ccatcgcgat
     3301 atggtctctt tattcacacc atataaatct gcaaagtact cattgcctgc ccaacaaaag
     3361 cctctttcat tacacaaggc cgttatctct ccgtataaca acttagtatt tggtttaagt
     3421 cttttgtcgt accttacgtt ggctggtata atcgcataat aacttcgatg ttctgtcatt
     3481 tttaccctcc aatatttaac tttttgattg ttttctggtt taatttgatc cctttgattt
     3541 gatatttatt tttgaaatta atcacaccta ttttgtgctt ctctgtgtga tggattctgc
     3601 agagtgctgc aaatgtgtac tctgcatgat caacttcttt gcgctttcgt cttcctagcg
     3661 ctttgtcaaa gtgatcgatg tcagctcctg ttttgccaca gatgcagcag actctttttg
     3721 tgatgcattt gtagaagtaa tattcttgat tcgcaggtaa aatctcatag ccttctttga
     3781 aaggaatatg atgttcaaag atgaaatcta agatgatatt tgctaagaca ttggcatcac
     3841 tcacagttgt attcgattcg tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac
     3901 gaaagtagaa gaattccttc cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc
     3961 ctatcagcgc atagatgaag tttcgttgct gtacggtgaa acgtctagga tcaataaaac
     4021 gaatttcaat gactcgatca ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt
     4081 tcacttcctc attgatggtt gcgcttatgt cttttccttt gaactttttc agaaccgctg
     4141 aatatgaatc gattaatggt ttaaacactc atatcacttc ttatctaatt cttttctctt
     4201 agctgctatt gctcgctcca tcaaggcaca ttgctcatag cttaactgtt caatagtttc
     4261 aacgttatca gctaagagcc ctaatttatc tgtctgctca ttaacatatt cgattaaggt
     4321 tttggtcata tctttaccca tctgctcatt gaaagcttct agaatcgtct ctagcatgtt
     4381 taatttcttt gtatcgattc taggtggtgt tggaatatct tccccttgaa atacatataa
     4441 tcccagtccg tgtagagcca atgctttcac aaagcatcgc ttcaatgagt tattgatttg
     4501 cattgcattt ggtttaacaa ctggttggtt tcgataatct aaaacaggaa ataattcggt
     4561 ttccgtgtgt cctttaaccg ttactgagac agatacataa gtcccagttt catccataag
     4621 aaaaggttta tattcctcaa caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg
     4681 tttgtactca ttaatagtta ccgttgcctg tggatcattc tttttcataa tctcccacgc
     4741 gtgagcccaa gataaataat caaaatttcc ttttttcttg agaattttat ttaacttacg
     4801 actaaaaagt ttttcaaagt tcgttgtccc tttgatttca ctcatcaaat tctgcctcca
     4861 tttcagcaat gtatttctta cctggtccgt aataagagat atcaatcaag ttatctctgt
     4921 cgtactcttc tagcgcatca atcaagccat cttcgatgac gtaaatatat tcaggtttat
     4981 tcgaatgctt cgatagatgg ataagataaa catgatccca aatagttaca tggttgccta
     5041 aatcatcttg atcccaagct agttcttcat tcgtcaaaag atttcgtctg atttttcgac
     5101 cacttgtttc ctcaattttc ggcttgcccc aatcaggatc aatcaaatat tgatctagag
     5161 tggaaagttc tttttccata tgctaaaatc tccttatgat gtgttttctt tgtgactctt
     5221 tgcttgccgg cggagtcact tttttatttg ttgccatgct ttttgcttgt caatatgttg
     5281 ttggcttagg atgattggtt tatagtattt ccaccagcaa ttagcaattg ccgtccctat
     5341 tcttagcgct tcagctctat tcattgtcat caccaaaaag tctctgttgt ctgttcagtt
     5401 gatcaatttc catacggatc gcagtttctg gcaaccacat ttcaataaat gaaacagcat
     5461 catcgaatct cttacgaggt aactcgccat atcttgggat tgaaaaggta cgtttaaatt
     5521 cagaccaaaa ttttgagaat acttttttgc tgatttcttc ataagctcgg ctttcttttc
     5581 cccctagaac tcccataact ttcatatttc ctttttgctt aatttcaaac tcttgttgtc
     5641 cgctaattcg catagtatct ttaagcatgg aaacatcttt tttaacatct ttcatttctt
     5701 ctaattgata gatcatcata tcttcaattg tttgaggaac agtattcttg cgaataacat
     5761 cttccatttc gttgaatgct tcaatgtatt tttgtttgaa gtagatagct ttctttcctg
     5821 taaaccccat agccagcaag aaaaatccat ctctactaat gaagaaaact cgtcgatttc
     5881 tgccatatga atctggttca ttaccttcta caaacatctg tccaaaattg gacacatctt
     5941 tttttagtgc atcaatatct cttaaaacat gttgatgttt tttctcgaag ctttctgcca
     6001 cttgtaagct cgtagtcaca gcttctttat ttttcaaaat tactaattct tgcattattt
     6061 cttctctcct ttttggtata atttagttaa aaactggtgg tgtttatttt gaattttttt
     6121 cacttaacaa tttttcaatg gatgtcaata ataagtttta tattgagtac gtttttaaca
     6181 gggataaaaa taaaaggaat gcgccctcaa ctagaagttg aacttaatgc ttcttacttc
     6241 gctgcagata tgatttatac aaaagtaatc atttctaact attcaacaga accagctatg
     6301 ttagttaatt tagaactttc atccgctaac ttaaaccgca gatggtcagc tactccattt
     6361 aagaaattaa ttgctaaagg aggatcagtc gatgataata gaatatactc tgaagctgta
     6421 ccgctaaata ttcctcctaa aagtgctata tcttgttacc ttgcatttga agtagggaaa
     6481 attaatttta ataaactcct taatcaccaa acaaaaatga tatttactct aaatagaacc
     6541 caaatccaca aaattgttga tatcaaaaat acaaacttcc caatagagaa attagtgaaa
     6601 gaattaaact gagtaatagt aatatacatt ctagtttctc ttttctattc atttttagtc
     6661 agccccctcg gttggctttt tcgctctgta ctcagcttca gcttcatcaa gccccataaa
     6721 aatccaaacc atgtaaacaa tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa
     6781 agcgtagatg attagcggtg cgctgaacac tagcgctcta ttaaattttc ccatccgctt
     6841 gcctccttaa gatttccgaa aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa
     6901 atagatatgt gtctcttcct tcaactggat aatagacaaa tccatttttg tttttctcga
     6961 tatcgataat atttctatat cttgggttct ttaaaactct agaagtaaac caatcatatt
     7021 ttctgttaat ccgttctagc acttctggca acgtcatcca tctaccagta tcatcagctt
     7081 tttttaactc ctcataatcc acttgggaga taattacata gccttctgga attgggattt
     7141 ttgcttctag atattgcatt agctgttcct ccctttacaa ttcgtacata gtgataatcg
     7201 aatctataat tctgtttgct tctgcagaag tctttttacc gtttaaaatt aaagataagt
     7261 agcttttgct aattccaaat ctttcagcaa gcatggtgta agttaagaac tttgaacttt
     7321 cgacatattc tttgattttt tctctatctc gttgagtgat ttctgcaatg tcagtcatac
     7381 taaaactcct ttctaaccaa tttcctctaa atccatttga gggtaatatc cttctttttt
     7441 tagtaattcg tagataaata gacgcccttt ctgtgtccat ttggtattca ttacaatttt
     7501 agtgccacca tcggctttcg ggatctcagt tgtatgagat tttgtgtatc cttggttcat
     7561 gtgttttttg cacaataacc attggttacc gacttttttc tgaataccta gtttatgaag
     7621 taatttattc atctgttgtg gagacatccc ataatctgct gcaatctgac taattgttac
     7681 tgaatctgta gaagataata tgctatctaa ataggagatt ttaggttcgt actctgcaat
     7741 cttttgttct gcgattaatc ttccagttct ttcttctttt agttgagttg ctaattgaat
     7801 gattgtatct ggattaagca aagcttcttc tactttttct ggagttagat aacctccatg
     7861 ttttctaatt gctggcaaca cttcacttgt tacccaacgt ttaaattttt tggcagaggg
     7921 aagttttgat tttaagatta aactgtacaa accagattcg ttgatgattg tcatctctct
     7981 tgattgacct gaggtcgtga ttcgcgaccc catcttatct tccaaatcta catgccgcga
     8041 caaagcatct ttagtgtttg aataacccaa aacactcgca acgtctttgc ctacaaaata
     8101 tggttcatca tttactaaaa ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg
     8161 tgtgttcata tttctcattc ctttctttta tattcgtaaa caaatttaac aacttttttc
     8221 taaattcaat tgacagtatc tgagtaatac tctatactaa gagcataatt aaataagaca
     8281 taaaaacatt gattttaaag ctttcttggc ggtctgcatt tacatatcaa tagtgttttt
     8341 tgttgtcttt ttagttgtta aatctgttta caagatatag tatagctcat tactcagaat
     8401 tagtcaaatt atttctgcgt atttttctaa actttttttg taaacattta gaaaggttgt
     8461 tttatcgatg aatacctatg aaataataaa agacttggcg aagaggaaaa agatatctat
     8521 acggcagtta gaaattaatt ttggatattc taacggttat ctagcaaagt ggaaaaataa
     8581 tactcctaat tcagatgaat taagtcggat tgctgactat ttcgatgtct ctgtagacta
     8641 ccttctaggt agagaagaaa gagaaacccc taaacatgtg gatttatcag aagacgatac
     8701 tgttttttct tttgatggaa aagaaatatc taaggagaca atgcgtaaag cgattgcaat
     8761 tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg tttgtatgta tttgaaattg
     8821 aaagaaatgt tgagcgaata caatttgaaa gtagtttata tggagatgaa agagcctggt
     8881 ttctactatc caaaacccag aatcattttt ttgaatgaaa atctatacgg tgaaactgca
     8941 gaagcctttc atttatctca tgaacttgca catttcagcg cctctcattt tgaattttca
     9001 gttttgtacg atacttctac aacttttcac tcaaaatttg aaacagaagc agataaaata
     9061 gctattctaa ttttattaaa tatctatatt gagaacgaac tgactgataa atctcagttt
     9121 aatctcgaaa aatttatgga gtattattct attcagaata agctcagata cacttgttat
     9181 gctgtctgcc aatgctattt taagaaaaaa tattcttacg caaggcaata tgtatgaata
     9241 catcaagaat tattaaaaaa accgatttca gcaaaacatt aaaaaagccc gtgctgcaac
     9301 acgggctctt tcctcatata tgagcttcta caaaaaaaat catatcacag aaatgaggaa
     9361 gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa aaaccttgat taaagtaact
     9421 gatgattcta tttcaattat tagaaaagga tttataaatt tagttaatca aggtattaaa
     9481 ggagaaaaaa ctatcccctt taaaaatata tcagccgtac aattaaaaaa accaggtatg
     9541 agtaatggtt atatacaatt taccttgcta ggcggaaacg aaagtcgcgg aggtatatta
     9601 gcggccacga aagatgaaaa caccgttatg tttacaaaaa aatattggaa tgaaatggaa
     9661 aatttaaaaa aatatataga gaagcaacaa tctatattag ataataattc caaagaaaca
     9721 caaatttcaa gtgcagatga aattaaaaag tataaagagt tgttagatga agggattatt
     9781 aatcaagaag aattcgattt taagaaaaaa gaattgcttg gtttataata ttcaatatca
     9841 aaagagaaag aggatctatt aatggacggt atatttggtt ttactggtat tgttttattt
     9901 attataggtt taatcatgtt aattgtaaga tttatcaaaa aaacaaataa gaaaacacct
     9961 atgattctcc ttattatcgg gattattttt actaccgtag gtttctcgct ttcctcatca
    10021 aacgaggcaa atgacaataa caaacaggaa agctcatcat ctgatactca aactactaca
    10081 tctaacagca gcaataaaag cctaactcaa tttgaagaat ttttagagac aaataatcat
    10141 gactggggtg ctttcttaga tagttattat tctattactc cagctactga gcaaaataca
    10201 gcttttacta aatatattag tggtaaaaca tatacttttg aaggaactgt tatcgaatca
    10261 atgactacta gaatagccat tatagcagac aaagagtacg atgataaaag ttggaatgat
    10321 atttctgcaa cacctaaagt ttcatatgta atttttgcga aggacttaaa caatgctgat
    10381 acctttacaa agggcgataa agtaactttt acaggagaaa ttagttcaag aggatcgaac
    10441 atagaaaaat cctatgctca atgggatatg attaatagta aagtaagtaa aaaataaatg
    10501 aaccacgccc cactaagagt tagattgtgc acaataataa aacctataca aaagagtgcc
    10561 atcaggagtc acaatgcttg taaaggaaat gacaaaatag gaggtaacta tggataatag
    10621 caatgaaaaa ctaaccaaaa aagaatatct tgaattaata gttgaaggcg gtattggagc
    10681 aattccttat attggtggcg ttgtgcagtc actttatttt gggtctaaaa acgagaagcg
    10741 tttcaaacgc atcgagaaat tctacaaaaa tttaaacgaa tcattagaaa acattcgcga
    10801 tcaaattcca gaagatgttt tcaacttaga gaacaaggac caactcattg gaatattcga
    10861 agggataaat gacgaggtag aaaaatcaaa ggcacaaaat aaaatagata tgtataaaaa
    10921 tctttataag aattgtttac ttcgaattaa caacgcttca tgggataacg aggagtatta
    10981 tcttcttgta ctgaatcaat taacatcgat tgagattcaa ttgttagcat gcttaatgag
    11041 tcgtggaaac gaaaatttca ctggaaatat cagcatgtct ggctattcac aagagctaat
    11101 agatggtagc ttaaatagat tatctgattt tggtctgtta gaaaaaacga ttagctccat
    11161 agtattaggt gatgtcggca aacaaaatat gggctacaaa atatctcatt taggaattca
    11221 atttattaac tcaactctta cttaagtttt ctttctaata tgacatctag acctatcagc
    11281 aatgcaacaa taaaaaacca gacataccaa taatcaacaa ataatctaaa ctctggaagt
    11341 tcagttattc ttttaaaaat cactttaatc ataataaaca cctctgattg gatgatctaa
    11401 tacacatcca attataccat taa
//
LOCUS       DAFFQY010000084        11347 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFFQY010000084 DAFFQY010000000
VERSION     DAFFQY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.084"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..936
                     /locus_tag="LRB02_002204"
     CDS             <1..936
                     /locus_tag="LRB02_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660379.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="LRB02_002205"
     CDS             939..3122
                     /locus_tag="LRB02_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL4660380.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="LRB02_002206"
     CDS             3119..4120
                     /locus_tag="LRB02_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL4660381.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="LRB02_002207"
     CDS             4135..5049
                     /locus_tag="LRB02_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4660382.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="LRB02_002208"
     CDS             5294..5410
                     /locus_tag="LRB02_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL4660383.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="LRB02_002209"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="LRB02_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL4660384.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="LRB02_002210"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="LRB02_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL4660385.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="LRB02_002211"
     CDS             9380..10642
                     /locus_tag="LRB02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL4660386.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11347
                     /locus_tag="LRB02_002212"
     CDS             10887..>11347
                     /locus_tag="LRB02_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL4660387.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK"
BASE COUNT         3741 a         1897 c         2376 g         3333 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaaa
//
LOCUS       DAFFQY010000085        11335 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFFQY010000085 DAFFQY010000000
VERSION     DAFFQY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.085"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(362..1480)
                     /locus_tag="LRB02_002213"
     CDS             complement(362..1480)
                     /locus_tag="LRB02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660388.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="LRB02_002214"
     CDS             1951..3258
                     /locus_tag="LRB02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL4660389.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="LRB02_002215"
     CDS             3377..4576
                     /locus_tag="LRB02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL4660390.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="LRB02_002216"
     CDS             complement(4603..5871)
                     /locus_tag="LRB02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL4660391.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="LRB02_002217"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="LRB02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="LRB02_002218"
     CDS             complement(7088..7591)
                     /locus_tag="LRB02_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4660392.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="LRB02_002219"
     CDS             complement(7714..8478)
                     /locus_tag="LRB02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL4660393.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8586..8861
                     /locus_tag="LRB02_002220"
     CDS             8586..8861
                     /locus_tag="LRB02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL4660394.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9299..10249
                     /gene="yidC"
                     /locus_tag="LRB02_002221"
     CDS             9299..10249
                     /gene="yidC"
                     /locus_tag="LRB02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL4660395.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10428..10628)
                     /locus_tag="LRB02_002222"
     CDS             complement(10428..10628)
                     /locus_tag="LRB02_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660396.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3400 a         2387 c         2002 g         3546 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcc cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagcaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttatctatta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 gtgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     7201 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaat tcggtgatct tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     7681 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     7741 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     7801 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     7861 tgcgaagtta tctgattttt ctactttgtc taatgcgtgt gcttcttcat ttaattctgt
     7921 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     7981 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     8041 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     8101 catcgtccct acatttccag gatcttgtac attatctaac aacagccatc ctcctgaaaa
     8161 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     8221 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     8281 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     8401 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     8461 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     8521 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     8581 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     8641 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     8701 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     8761 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     8821 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
     8881 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
     8941 acttaatgcc gtagatgcaa gcttttgaac accgtcgata aggttattct tgaggcgctt
     9001 tgcctcaaaa attaaggaca gcagtgaaaa agctgatcta tcacttgcag tgagttcatt
     9061 tgactccgct ttttcgggca gaaaaaaagt ttttagttta ttaaagtaag aaagaaacac
     9121 ttggaatcat tctacttttt cctagtattt ctttcttgat tttttgtctt tttttcaaaa
     9181 tttttcgcta cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc
     9241 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
     9301 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
     9361 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt
     9421 actcgtccat cctatgggcc aagcgattac atatctagtt gaaaatttca attggtctta
     9481 tggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat
     9541 tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
     9601 tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca
     9661 agcagaaatg cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg
     9721 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac
     9781 agaaggtatc cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact
     9841 tgttgccatt gcaggggtcg cttatctgct tcaaggatac atctcaacga tcggtattcc
     9901 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
     9961 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag
    10021 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
    10081 agaagagttg aaacaaaacc caccaaaaca agtcgtcact ccaagaattg atgtgacaga
    10141 atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa atcaagcaac ccaaaaacag
    10201 tacaaacaat aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
    10261 gagtgtaata aaaattcatt aacagtttta gcaattaaaa atatcgacca agatttttga
    10321 aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc atcgattgat
    10381 cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta tctatcaacg
    10441 tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ccttaatatt ttgttttcct
    10501 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg cgccactccc
    10561 ttcgcccatg ttgctcgccc acataagaca ccattctcca taaaggacaa cgcggagata
    10621 ccacccatct aaataacatc cataaacctt tctagtagaa gaatagtgat agtatccatc
    10681 cctttttcta ctgggaatac aattacacga ttcaaagctt gatacacaac atttcccttc
    10741 ttataaaccg ctattgtaac cgaattgttt tgaatcacct ttttgggata gaaactggct
    10801 cctttccgca acttcttaaa tgttggtttc ttttatactt tctttgtagc aactacaaat
    10861 ttcttcaaat ctctaaatgt ttgcataaca accgcctttc tgacttttac tcaggatggt
    10921 atttaaaaat cagcaaaata aggcttctct ccatcttttt tatttcctag ctgctttaaa
    10981 gacactcttg ctgtttttta atctctatca agggggctct gaatctattt tgatgaactt
    11041 atcttattaa tttctcaaat gagctgtcag acacaactgt attttttcaa tctcataccg
    11101 gacaactatg ccagcttaaa aaaatttcct ctggcaaaac ttctttattg aaaaatgaca
    11161 atgaaaaaag accattcttt ttgttgaatg ctcattgtgt aaacatattt cctgcgactg
    11221 ctcttcgaac cattctcagt gcaatcccta atttatctat taaatcttct tttagttcct
    11281 catctttcat attcaaaatt tcctttgtat gttacacaag tttgttctct aaata
//
LOCUS       DAFFQY010000086        11253 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFFQY010000086 DAFFQY010000000
VERSION     DAFFQY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.086"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(40..1419)
                     /locus_tag="LRB02_002223"
     CDS             complement(40..1419)
                     /locus_tag="LRB02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HBL4660397.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="LRB02_002224"
     CDS             complement(1440..2987)
                     /locus_tag="LRB02_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4660398.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="LRB02_002225"
     CDS             complement(3236..4015)
                     /locus_tag="LRB02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4660399.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="LRB02_002226"
     CDS             complement(4090..4548)
                     /locus_tag="LRB02_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4660400.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5834)
                     /locus_tag="LRB02_002227"
     CDS             complement(4560..5834)
                     /locus_tag="LRB02_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBL4660401.1"
                     /translation="MEDLIMKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREAR
                     NYLINQMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVV
                     TALETMRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVK
                     NRHGQTKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIP
                     GIGRFKARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIK
                     PNSNQFVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARIN
                     YSNPTNPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKG
                     GITHHPDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="LRB02_002228"
     CDS             complement(5935..6249)
                     /locus_tag="LRB02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660402.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="LRB02_002229"
     CDS             complement(6395..7891)
                     /locus_tag="LRB02_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL4660403.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="LRB02_002230"
     CDS             complement(8069..8548)
                     /locus_tag="LRB02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBL4660404.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            8715..8864
                     /locus_tag="LRB02_002231"
     CDS             8715..8864
                     /locus_tag="LRB02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660405.1"
                     /translation="MRKVDEYIKKKSQKSKVWQRTFELEYKKLSDSLKKDTYNPNEQS
                     KADNQ"
     gene            complement(8924..9169)
                     /locus_tag="LRB02_002232"
     CDS             complement(8924..9169)
                     /locus_tag="LRB02_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660406.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="LRB02_002233"
     CDS             9274..10047
                     /locus_tag="LRB02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660407.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="LRB02_002234"
     CDS             complement(10276..10524)
                     /locus_tag="LRB02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4660408.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="LRB02_002235"
     CDS             complement(10623..10937)
                     /locus_tag="LRB02_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660409.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
     gene            complement(11074..11220)
                     /locus_tag="LRB02_002236"
     CDS             complement(11074..11220)
                     /locus_tag="LRB02_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660410.1"
                     /translation="MILNNPEYIESGKAETVNYYYELPSEIQNYQIVIREADGYFVGL
                     PFTL"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DAFFQY010000087        10767 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFFQY010000087 DAFFQY010000000
VERSION     DAFFQY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.087"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            79..1125
                     /locus_tag="LRB02_002237"
     CDS             79..1125
                     /locus_tag="LRB02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL4660411.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="LRB02_002238"
     CDS             1474..2106
                     /locus_tag="LRB02_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL4660412.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="LRB02_002239"
     CDS             2694..4133
                     /locus_tag="LRB02_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4660413.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="LRB02_002240"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="LRB02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="LRB02_002241"
     CDS             4895..5110
                     /locus_tag="LRB02_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660414.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="LRB02_002242"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="LRB02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="LRB02_002243"
     CDS             complement(5770..6681)
                     /locus_tag="LRB02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4660415.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="LRB02_002244"
     CDS             complement(6700..7704)
                     /locus_tag="LRB02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL4660416.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="LRB02_002245"
     CDS             complement(7701..9824)
                     /locus_tag="LRB02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4660417.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="LRB02_002246"
     CDS             complement(9829..10749)
                     /locus_tag="LRB02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660418.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DAFFQY010000088        10737 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFFQY010000088 DAFFQY010000000
VERSION     DAFFQY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.088"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..339)
                     /locus_tag="LRB02_002247"
     CDS             complement(<1..339)
                     /locus_tag="LRB02_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL4660419.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIED"
     gene            489..971
                     /locus_tag="LRB02_002248"
     CDS             489..971
                     /locus_tag="LRB02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL4660420.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1008..2018
                     /locus_tag="LRB02_002249"
     CDS             1008..2018
                     /locus_tag="LRB02_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL4660421.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2133..2846)
                     /locus_tag="LRB02_002250"
     CDS             complement(2133..2846)
                     /locus_tag="LRB02_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660422.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3036..3626)
                     /locus_tag="LRB02_002251"
     CDS             complement(3036..3626)
                     /locus_tag="LRB02_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL4660423.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4110..4337
                     /locus_tag="LRB02_002252"
     CDS             4110..4337
                     /locus_tag="LRB02_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL4660424.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4353..5519
                     /locus_tag="LRB02_002253"
     CDS             4353..5519
                     /locus_tag="LRB02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL4660425.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5753..6796
                     /gene="hrcA"
                     /locus_tag="LRB02_002254"
     CDS             5753..6796
                     /gene="hrcA"
                     /locus_tag="LRB02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL4660426.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6890..7453
                     /gene="grpE"
                     /locus_tag="LRB02_002255"
     CDS             6890..7453
                     /gene="grpE"
                     /locus_tag="LRB02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL4660427.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7504..9333
                     /gene="dnaK"
                     /locus_tag="LRB02_002256"
     CDS             7504..9333
                     /gene="dnaK"
                     /locus_tag="LRB02_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL4660428.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9484..10650
                     /gene="dnaJ"
                     /locus_tag="LRB02_002257"
     CDS             9484..10650
                     /gene="dnaJ"
                     /locus_tag="LRB02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL4660429.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3651 a         1817 c         2271 g         2998 t
ORIGIN      
        1 atcttcaata ttaaatgaaa tcaattgttc aatcaattcc cagttgatta cacttttcca
       61 aggaactttg atgacgttat ccgtatatac gtagcctgct tcttggatat ttttttcaag
      121 ggcacgaatc gctgcttttt ccggtgcgat agaaaaatga tttttagcaa aactaaatgc
      181 aataataaag gtaccatggt cagtaaacat aggttggttc cattttacag ttgtgtctag
      241 ctggggatac gtttctgtta tcttagcaaa aaggtctttg atcttttgtt gatgttcttt
      301 ttctttgatt tgattgatga tcggctgaaa tttttccatt ctaaaacgcc tccattaagt
      361 ggtttgattt attttaccag aaaaacaata gttatgtata aaaaacgaac aaaacacaag
      421 taatgttgta accgctatat ttatatgata agatgtctca aataataaaa tagaaaagag
      481 acataaaaat gattgacttg gcaaagaaat tcttacacga aaatatatat tttgagttgt
      541 atcatcatcg agccatttat acaaatgaag atgctttggt aataaagaaa gaacaaggat
      601 ttacaggtac agagaccaaa agcttattct taaaagataa aaaagataat aactacgtat
      661 atctgacttt tactacgaaa caaacggatt tcaaagcatt agggaagatc gtcggtaaaa
      721 ggctttctat tgtttcatca gaacgcatgg aagaacaaac gggacaaaag cagggcgctg
      781 tttctccgtt tggttatgaa aaacagattc cgattattat tgatgaagag ctcttatcaa
      841 atgaaaaact tgtttttgca ccaggtagac cagatcaaac gatggtcatc caaacggttg
      901 acttaaacaa aatcatccag ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa
      961 aaaacaatta accaagtaaa atagtgcata cttggaggtg aagcaacatg ataagttatc
     1021 cattattaga aagaaatgca acgattgggg ttactgcacc atcctcaggc gtatcagagg
     1081 aacaccattc gttactcgaa tcagctttaa agagaatgga agaaaaatct ttccaagtag
     1141 ttactggaaa gacgctttgg acgcaagata aggaaaaatc aggtccagca agaaaacgag
     1201 cagatgaatt caatcaaatg atgcaagatg aaaatatcca aatgatcttt ccgacttggg
     1261 gaggagaatt attgattgag attctcgatt ttgtcgattt tgatgctcta caaccaaaat
     1321 ggatagtagg ttattcagat attagtacgt tgctacttgc tgtcacatta aaaacaggga
     1381 tagccactgc acatggaact aaccttattg atttaagagg agagtacttg gatgaaacga
     1441 cagcaatgtg ggaaagtgta ctgaaaacaa gagaaggaga atcgattcta cagcaatctt
     1501 ctgaaaagta tcaaaaagtg tggccccatg agcattcatc atcttctgtt ttccatctca
     1561 ctgaaccaac aaattggcaa tcagtcagcg gagatgcagt aaggatgaag ggacgattat
     1621 taggtggttg tattgatacg atcaggcatt taatcggtac tccttatgga gatgtccaat
     1681 cttttaaaag aaagtataat gatggaaagc cgatcttgtg gtttctagaa aactgtgagc
     1741 tatcgtcagt tgatttaaga agatcacttg ttcagatgaa actggcaggc tggtttgatg
     1801 gcagctcggg gatcatgttc ggtagaagtg cggcagagga tttgttagat tataaaatga
     1861 aggatgtata cgaagatctg tcaaaggaat taaaactacc gatcttgtat gatattgatt
     1921 gtggtcatgt accgccacaa ctgacattga ttaatggtgc ctatgcagaa gtagcggtag
     1981 aagatagaaa agcgactgtt ttgcaaacgt ttatttaaaa aattttttcc tgccttggag
     2041 tctatcaaag accaataaat ctaaatagaa aagaaagtga aagaagctga caaaatgctg
     2101 tcagcttctt tttgtataac atattagaaa gtctaattat ctataactgt cacatcgatt
     2161 aactgtttca cgtcattggg atttcctgta ggaatagcta ctacagatgc attatcaatg
     2221 atttcttgag ttgtttctct aatgaacgct ggataagatg tttgatctgc aggcatttgc
     2281 caatgtgaag gaactggata actagtgatt gtcccgtcat ggttacttga atacactaaa
     2341 actccctggt aactgtattt cccagtgagc ataaccacat ctttcggata gactgcactc
     2401 cctgcatcat aaggggcgat aggtgttcct gcaggataat gaataacatg taattcgaaa
     2461 tcacccgatg tatctctaaa agtattacct ctaatagcta gccaaacacg tgcgtactca
     2521 atttgtagat cagaatatcc tgctaacgga tctattgact gagaagactg tgaggaggtt
     2581 gtgtctattg ttgaagaaga aatatttgtc tcaactgtac tactagatgt agaagaagta
     2641 ttgggctctg agactttact gcttgttgaa gatgtattcg tttcagtaga agctgatttc
     2701 tgagtacttg atttttcagc tgaactggtt tgtctgattg aacttttgtc tgtggtcgca
     2761 gatggatcac tattttcttt actaggagac gtacatccta caaggaataa agagaccaac
     2821 aataaaataa aacctaactt tttcatataa aatacctcat tattttttta ataccagtat
     2881 agcataatcc tgaatttttg aatccgtaaa accttgtcta tgaatataaa tagagtgtat
     2941 cttctttgat aaatgtcaga aaaagtgtga caaataatca tcagtaaaat tacttgtcac
     3001 actattgata aggagtattt aattgaagag aatgattagg agaaaggatc tccgtacttt
     3061 ttatagtaat catagaatcc aagtttgtat agacgaatcg ttttttctct tatttcttct
     3121 gtttcaaaat ggactgcttg aagaatcaag tctgtgaatt ctataggagc tgttccattc
     3181 gcagtaatca aattacgatc tttcacagct tgttgttcta taaaatcttc tttattctga
     3241 taattttccg attctaacca aggaaattgc gcatttccgg tgtgtttgta accttgcaat
     3301 aatccgctct gagctaaata atcaactgcc ccacaaatag cagctactgg tacgtctgtt
     3361 tttaacaatt tatcgattat ttgatataaa tgatgatttt ttatgtccca tgaattgcca
     3421 ccgatcataa taaaaaggtc aactttttca ggtatttgtt cgattaagta atcgattttt
     3481 gtttttaatc ctccaatgga agtgacctct gattggatag aagcagtttt gttttgccat
     3541 ttctcatttt ggtttagtgt gctagtaata taagcacctt cccaatcggc atattcctct
     3601 aatagaaacg taatcgcagt tttcattttc aagctctcct ttactatcta taacaaggac
     3661 aacagtttta acctacagac attcattaaa attgggtagc gacagcgaaa acaacaattc
     3721 ttaagtttta gacagagtat acagatagtc aacttaatta aatacgaact aaataaaaat
     3781 gacaggtaat catttttgtt taccagcatt ataacatttt gcatacatgg atgacaaaag
     3841 aaccaattcc cacttgcttg agtacaatat taaaagaaac tggaaggcaa aaagtaatta
     3901 tagaaacgaa aaataaatca tatcgggcac tcagacatga aaacaaccct agagatatat
     3961 aactaggtaa ccagtttaat gaaagaaaaa atcatccaag aagtcgattc ttggattttt
     4021 tagtggattt gttcgccaaa tacgtttagt atgcgtatat gagaggttgt aagcaaagcg
     4081 ttgtggtctg agcacttaag gaggaatctg tgaaaatagc ttttcatatt tttgcagatt
     4141 ttgacttaat gccgaaggtc agctttgcga ttaccgttta tcgagaggct gtaaaagcac
     4201 cgtgtagctc cgatgacaat aagcaagcat ttttagagct aggtttcctt gattctaaat
     4261 atgcggctta tttcaatgag ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc
     4321 acgacttttc gacgtaagga gaaagaattt caatgataga aacaataaat cccgcagaaa
     4381 acaaggtttc tgcgtacatt catatcccat tttgcgagca tatctgctat tactgtgatt
     4441 ttaataaagt ttttttagaa gggcagccag tagatgagta tgtggaaatg ctgctgaatg
     4501 aaatgaaaat aatgctagaa agtcatccga cagcgaattt agaaacactt tacgtagggg
     4561 gcgggacacc aacttcttta tctgctaaac aacttgatcg actgttatca ggtgctagag
     4621 agatcctgcc ttttcaaaat ggtaatgagt ttactgtaga agcgaatcca ggtgatttga
     4681 cccgtgaaaa acttcaagta atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc
     4741 agacatttga taatcgctta ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt
     4801 atcaaactat gcaattttta gaagcagaaa attttaccaa tgtaagcatc gatttgattt
     4861 atgcattacc gggacaaaca ctagaaggat atctggatac tttggaaaaa gctttggctt
     4921 tggatctgcc tcactattct ttgtactcat tgatcctcga aaacaaaaca atgttcatga
     4981 actgggtacg acaaggaaga ctcgaactgc ctgatcagga aacggaaacc agaatgtttg
     5041 aagagacgat tgaagcaatg gaaaagcatg gacgtcacca atatgaaata agtaactttg
     5101 ggctctccgg acatgaatct caacataacc tcatgtactg gaacaatgac cactattttg
     5161 ggtttggagc tggtgctagc ggttatcttg gagatattcg ttaccgcaac aaaggaccaa
     5221 tccaacacta tttacgccca ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat
     5281 tgacacgaaa aaatcaaata gaagaagaaa tgttcttagg actaagaaaa aaaacaggga
     5341 ttactaaaaa acatttctat gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg
     5401 tgctaaggga attagagcaa gaagggttac ttgttaatga agaagaccgt gtttatttga
     5461 caccaagagg gacctttttg gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt
     5521 ttaaagaata tttgtttttt atctgaattt aaataaaaaa agttcaaaaa tacaataaag
     5581 gactattgaa acgttgatat aactggattt acatcaatta tttagaatta gcactcttgt
     5641 gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt
     5701 tataagtgga ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac
     5761 aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc
     5821 cgttggttcc aaaacattga tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa
     5881 tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg
     5941 gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt
     6001 agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa
     6061 tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc
     6121 attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa
     6181 tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac
     6241 tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg
     6301 tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat
     6361 tcttcataaa tatttccaaa cgacagaagg gatgtctcag ttatttgaca ccgttcttgg
     6421 tcatgttttt gaagataagg tttatgtcag tggacaaatg aatctcttgg attatgagcc
     6481 ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact
     6541 tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg
     6601 caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg
     6661 aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct
     6721 agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc gttcgcttga
     6781 cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt
     6841 agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa
     6901 tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga
     6961 agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt
     7021 aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa
     7081 tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt
     7141 agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc
     7201 agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa
     7261 cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa
     7321 tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt
     7381 agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat
     7441 tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta
     7501 attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg
     7561 gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt
     7621 gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca
     7681 aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat
     7741 gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa
     7801 ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct
     7861 tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa
     7921 gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca
     7981 gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc
     8041 cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt
     8101 ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac
     8161 agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa
     8221 gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca
     8281 ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta
     8341 acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt
     8401 ttatcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca
     8461 gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat
     8521 gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat
     8581 gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc
     8641 actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact
     8701 gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct
     8761 gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt
     8821 attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg
     8881 aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca
     8941 gacgaagaaa tcgaacgtat ggtaaaagat gcagaagcaa atgcagaagc agataaagct
     9001 cgtaaagagg aagttgacct acgtaatgat gtagatgcat tgctattctc tgtggacaaa
     9061 acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct
     9121 cgcgatgaat tgaaagccgc tgttgaagca aacaacatcg aagaaatgaa aactaaacgc
     9181 gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agcggcacaa
     9241 caacaagctc aagaaaaccc agaagcagct caaggcggag cagatgatgt cgttgatgct
     9301 gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc
     9361 caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc
     9421 aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag
     9481 cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa
     9541 gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa
     9601 gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac
     9661 ccgcaaaaac gcgctgcata tgaccaatac gggcatgcag gcacagatcc aaattacggt
     9721 gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt
     9781 ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat
     9841 gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt
     9901 tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtggg
     9961 aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg
    10021 attaatgtag aacgccaaac accacttgga cgagtgatga gccgtcaaac ttgtgatgtt
    10081 tgtcatggga cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat
    10141 gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa
    10201 atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat
    10261 gtggtcttcc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat
    10321 gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc
    10381 catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta
    10441 cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta
    10501 aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct
    10561 gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa
    10621 gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaacgcta agtcattaat
    10681 aattaaaaac cagacgaaca atcatatgtt cgcctggttt ttttgtagtt gtcatgt
//
LOCUS       DAFFQY010000089        10451 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFFQY010000089 DAFFQY010000000
VERSION     DAFFQY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.089"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            105..323
                     /locus_tag="LRB02_002258"
     CDS             105..323
                     /locus_tag="LRB02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660430.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(612..1124)
                     /locus_tag="LRB02_002259"
     CDS             complement(612..1124)
                     /locus_tag="LRB02_002259"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL4660431.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1138..3435)
                     /gene="recJ"
                     /locus_tag="LRB02_002260"
     CDS             complement(1138..3435)
                     /gene="recJ"
                     /locus_tag="LRB02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL4660432.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3577..4020)
                     /locus_tag="LRB02_002261"
     CDS             complement(3577..4020)
                     /locus_tag="LRB02_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL4660433.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4021..4809)
                     /locus_tag="LRB02_002262"
     CDS             complement(4021..4809)
                     /locus_tag="LRB02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4660434.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4806..5747)
                     /locus_tag="LRB02_002263"
     CDS             complement(4806..5747)
                     /locus_tag="LRB02_002263"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL4660435.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5924..6511)
                     /locus_tag="LRB02_002264"
     CDS             complement(5924..6511)
                     /locus_tag="LRB02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL4660436.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6732..8039)
                     /gene="obgE"
                     /locus_tag="LRB02_002265"
     CDS             complement(6732..8039)
                     /gene="obgE"
                     /locus_tag="LRB02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL4660437.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8333..9343
                     /gene="gap"
                     /locus_tag="LRB02_002266"
     CDS             8333..9343
                     /gene="gap"
                     /locus_tag="LRB02_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL4660438.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9428..10363)
                     /locus_tag="LRB02_002267"
     CDS             complement(9428..10363)
                     /locus_tag="LRB02_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4660439.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2894 a         2358 c         1811 g         3388 t
ORIGIN      
        1 aattatagag ccatttttat cacttccctc tttacaaacc gaacaaatat tcgtatacta
       61 aatatacgaa caattgttcg cataaattaa agaggtgaga attcatgaaa tcaatgagac
      121 gtcttggctt agtattaact gttttgtttt taggtattat tattggtaat ttcggcatac
      181 gtccagcttt acttgtgttt gttccgttat ttactttgtg gttcatgttg tgggatgaaa
      241 agaagtaccg tcaatctgag cggcgcagaa ttcatgaaga tcatttctat catgagccct
      301 actacagatc caggcaatca taataaggat tgactgctaa ttattcaaaa gacaagaagt
      361 taaactttta cgggggtgtg acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt
      421 tctaaagcca gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct
      481 actggtcttt gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga
      541 atctgacttt tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt
      601 cttttttctg cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt
      661 ccatggagat ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt
      721 tcgatcagtt cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc
      781 agcacacgtt gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat
      841 tctaatccat aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg
      901 gcaaaaccga ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct
      961 ggcccaacta ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct
     1021 tcccgataag cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt
     1081 gacggatagt cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta
     1141 ggttgttaac catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt
     1201 gattttattg agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct
     1261 agcgactttt tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg
     1321 tatcataaaa attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt
     1381 atgacgtata tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc
     1441 tcttgtgcct accccattca aataggcctc atcaggtgaa atccccatca gataaacacg
     1501 attgaatgaa taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat
     1561 aacaactaga gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc
     1621 gaaaacaatc gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa
     1681 tgctaaataa aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca
     1741 atcaaatact tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg
     1801 cccgttccat tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc
     1861 ttcttcttga gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag
     1921 gacaaatttc agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg
     1981 tcgaaataaa aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc
     2041 atcaattcgc tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg
     2101 ccctctcatc aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat
     2161 agtgacattt tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt
     2221 aaataaatct cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc
     2281 agatccttta gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc
     2341 attcattatt ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa
     2401 tagatggata tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac
     2461 gattgctttt ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc
     2521 ttcatcaaac gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc
     2581 atttagtctg ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac
     2641 gccgctttct tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat
     2701 tcctaatgaa acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc
     2761 tatcgctact aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt
     2821 gaatgctact ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg
     2881 gtgaacaatc gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat
     2941 cacgtctaca ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc
     3001 aacagtgatg atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg
     3061 tccataccca tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc
     3121 cagtgtttct ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac
     3181 gaggattttt tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc
     3241 gtgtatcaga tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc
     3301 cttttcctca tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag
     3361 ttcacggatc atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa
     3421 atttgcttgt ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa
     3481 aaaaagcaga aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac
     3541 ctgatctttt gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg
     3601 tttgggcgat tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt
     3661 cgcttgtaac tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg
     3721 ttgctgttct tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat
     3781 ttcctgagtc agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa
     3841 aaatacaatc agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga
     3901 aaaggaagtg aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac
     3961 aataatcaaa actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac
     4021 ctactttttg ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata
     4081 aaattttgat gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat
     4141 attgcggacg atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc
     4201 caaatagctt ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt
     4261 cactgtcgta acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc
     4321 aaggactgca aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc
     4381 catagaggcg atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt
     4441 ttgtgtcaaa accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag
     4501 gtcgaatgta aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac
     4561 gcgcccgact tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac
     4621 gtctaccttg aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc
     4681 ttctacaaat gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc
     4741 ataacagatt tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc
     4801 taaattcatg cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata
     4861 atcgtcgtgt tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct
     4921 aaatatctcg cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact
     4981 tcagcagctt gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta
     5041 ttaaatgtgc tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt
     5101 gtttttctcg tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa
     5161 tcttttccat tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg
     5221 ccgtacagtg gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct
     5281 ttatgatcat gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt
     5341 gtataaacag aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat
     5401 ttcagcggat aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct
     5461 atcccttttg gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg
     5521 atgagcccgg gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg
     5581 atttttctcg gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg
     5641 aacaaccaaa cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta
     5701 tgttttgcag gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact
     5761 ttctttaagt tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag
     5821 aatgggttca aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat
     5881 aaaaacagca gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag
     5941 aactttcatt gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt
     6001 ctttcgcatg attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat
     6061 ccaagcgagt aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt
     6121 cctcttcatc agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa
     6181 agtcgaacac ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc
     6241 ctccggcaaa ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc
     6301 cgattaatcc acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt
     6361 tgaattgatg tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg
     6421 aacgaacagc atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt
     6481 gaaaatttaa aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt
     6541 atttatttta cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa
     6601 tagggctgtg acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag
     6661 ctgatcactt ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt
     6721 tatttgcttg cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca
     6781 tctttcgcac cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca
     6841 aaacgcatca ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc
     6901 tcaccagata agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct
     6961 ggattgaatc catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct
     7021 ggcgttactt ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc
     7081 actccagaaa ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc
     7141 agctgttctt tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc
     7201 acgatgattt gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg
     7261 gcaagatagt cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa
     7321 attactcgtg ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa
     7381 gctccttcaa tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta
     7441 accattccta gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca
     7501 gatgaaataa cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg
     7561 tctgccaata ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca
     7621 gcaatttccg gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt
     7681 ccaccttttg ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca
     7741 gtctccgcat ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca
     7801 cgaccgtgca tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg
     7861 aaccgaaaat ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca
     7921 cgaccaccgt ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca
     7981 accattccgt ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg
     8041 gacataatta agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata
     8101 aacaaaagtc ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa
     8161 gattgtttac cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac
     8221 caagtataat tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta
     8281 ttaattgagg atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt
     8341 aaaagtaggt attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga
     8401 agtctcagat gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc
     8461 acaattattg caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga
     8521 agactcaatc gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa
     8581 aatcccttgg gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac
     8641 atctgaagaa aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc
     8701 cccagctggt gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa
     8761 cgacaaaatc atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt
     8821 cttaaataat gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc
     8881 cacacaaatg ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc
     8941 tgctgacaat actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc
     9001 tgagctacaa ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt
     9061 aactgaattg gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat
     9121 caaaaaacac acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag
     9181 cgatgtcatt ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac
     9241 tgctggtgat ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac
     9301 ttgccaaatg attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga
     9361 taaaagaagc ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta
     9421 aactcgttca atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag
     9481 gatctgtctt gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc
     9541 ccttcgttgc acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc
     9601 catgtataaa gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac
     9661 cgaaaaagta gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg
     9721 ctggaagact aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac
     9781 cgcaatcttc aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa
     9841 ctgttgctgc tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt
     9901 ctagcacttt gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt
     9961 aatccccttc gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat
    10021 catgaaacat ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca
    10081 caagagctgt tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac
    10141 cgtcttttga agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg
    10201 catccgtttc tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct
    10261 cagaaggcac aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca
    10321 gagctaaagc gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt
    10381 tctctttttt catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc
    10441 atttttataa c
//
LOCUS       DAFFQY010000090         9796 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFFQY010000090 DAFFQY010000000
VERSION     DAFFQY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.090"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(71..2386)
                     /locus_tag="LRB02_002268"
     CDS             complement(71..2386)
                     /locus_tag="LRB02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4660440.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(2571..3431)
                     /locus_tag="LRB02_002269"
     CDS             complement(2571..3431)
                     /locus_tag="LRB02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL4660441.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(3490..4011)
                     /locus_tag="LRB02_002270"
     CDS             complement(3490..4011)
                     /locus_tag="LRB02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL4660442.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            4289..5050
                     /locus_tag="LRB02_002271"
     CDS             4289..5050
                     /locus_tag="LRB02_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4660443.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            5076..7667
                     /gene="mprF"
                     /locus_tag="LRB02_002272"
     CDS             5076..7667
                     /gene="mprF"
                     /locus_tag="LRB02_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL4660444.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(7855..9042)
                     /gene="tuf"
                     /locus_tag="LRB02_002273"
     CDS             complement(7855..9042)
                     /gene="tuf"
                     /locus_tag="LRB02_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL4660445.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIITE
                     IEA"
     gene            9410..9766
                     /locus_tag="LRB02_002274"
     CDS             9410..9766
                     /locus_tag="LRB02_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4660446.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         2892 a         2015 c         1742 g         3147 t
ORIGIN      
        1 attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc
       61 tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt
      121 acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc
      181 ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta
      241 cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa
      301 ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc
      361 cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg
      421 catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat
      481 cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga
      541 tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa
      601 atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc
      661 ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat
      721 tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc
      781 ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt
      841 tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc
      901 gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat
      961 cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag
     1021 aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt
     1081 ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt
     1141 cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg
     1201 ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt
     1261 acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga
     1321 ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag
     1381 tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg atggctttct
     1441 cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc
     1501 ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt
     1561 gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta
     1621 gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa
     1681 ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa
     1741 taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa
     1801 tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc
     1861 ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc
     1921 cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc
     1981 gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat
     2041 ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta
     2101 cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat
     2161 aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac
     2221 ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat
     2281 tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc
     2341 cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca
     2401 ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa
     2461 acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc
     2521 aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat
     2581 tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga
     2641 caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt
     2701 gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg
     2761 taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca
     2821 atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac
     2881 accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg
     2941 gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat
     3001 aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct
     3061 cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca
     3121 aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt
     3181 agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg
     3241 aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct
     3301 gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc
     3361 acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc
     3421 cttttttcca ctatttgcgc actccttctt tcatttaatt ttattcatga ttgaacattt
     3481 cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac
     3541 aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa
     3601 ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag
     3661 cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac
     3721 ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc
     3781 ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc
     3841 agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc
     3901 tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc
     3961 catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac
     4021 cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat
     4081 gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt
     4141 tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct
     4201 gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc
     4261 gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt
     4321 aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta
     4381 ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg
     4441 ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga
     4501 tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc
     4561 cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt
     4621 tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg
     4681 attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat
     4741 cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa
     4801 aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa
     4861 aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca
     4921 ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg
     4981 acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc
     5041 tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc
     5101 tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg
     5161 tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg
     5221 ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac
     5281 ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt
     5341 acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt
     5401 ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga
     5461 tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc
     5521 tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat
     5581 ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga
     5641 aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt
     5701 tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga
     5761 ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta
     5821 tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg
     5881 gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca
     5941 ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc
     6001 gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct
     6061 tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag
     6121 agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta
     6181 gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac
     6241 gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg
     6301 attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta
     6361 aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa
     6421 tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt
     6481 tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt
     6541 tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc
     6601 ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc
     6661 ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt
     6721 ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata
     6781 aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag
     6841 cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag
     6901 agattgttat gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt
     6961 atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct
     7021 tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg
     7081 agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag
     7141 ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca
     7201 aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata
     7261 aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg
     7321 atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt
     7381 tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga
     7441 ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat
     7501 ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt
     7561 ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac
     7621 aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg
     7681 atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc
     7741 tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg
     7801 accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc
     7861 ttcgatctct gtaatgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt
     7921 cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc
     7981 aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg
     8041 gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt
     8101 tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc
     8161 aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt
     8221 atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc
     8281 tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc
     8341 acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac
     8401 tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt
     8461 atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc
     8521 ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg
     8581 aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa
     8641 gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg
     8701 aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc
     8761 tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg
     8821 tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc
     8881 tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac
     8941 tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat
     9001 gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa
     9061 ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact
     9121 aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg
     9181 tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct
     9241 tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt
     9301 tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca
     9361 atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt
     9421 aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta
     9481 tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac
     9541 ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa
     9601 agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa
     9661 tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa
     9721 ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa
     9781 attttattag ataaaa
//
LOCUS       DAFFQY010000091         9347 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFFQY010000091 DAFFQY010000000
VERSION     DAFFQY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.091"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            11..538
                     /locus_tag="LRB02_002275"
     CDS             11..538
                     /locus_tag="LRB02_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL4660447.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="LRB02_002276"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="LRB02_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL4660448.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDCVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="LRB02_002277"
     CDS             2005..3162
                     /locus_tag="LRB02_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL4660449.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="LRB02_002278"
     CDS             3332..4792
                     /locus_tag="LRB02_002278"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL4660450.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="LRB02_002279"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="LRB02_002279"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL4660451.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="LRB02_002280"
     CDS             5647..7059
                     /locus_tag="LRB02_002280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL4660452.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="LRB02_002281"
     CDS             7056..7451
                     /locus_tag="LRB02_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL4660453.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="LRB02_002282"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="LRB02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL4660454.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="LRB02_002283"
     CDS             8308..8838
                     /locus_tag="LRB02_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL4660455.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..>9347
                     /locus_tag="LRB02_002284"
     CDS             8915..>9347
                     /locus_tag="LRB02_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4660456.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIE"
BASE COUNT         3187 a         1709 c         2113 g         2338 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattgtgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaatcg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tacaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgagcctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcatta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcgattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaag
//
LOCUS       DAFFQY010000092         8576 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFFQY010000092 DAFFQY010000000
VERSION     DAFFQY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.092"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(312..842)
                     /locus_tag="LRB02_002285"
     CDS             complement(312..842)
                     /locus_tag="LRB02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4660457.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="LRB02_002286"
     CDS             complement(1024..1680)
                     /locus_tag="LRB02_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL4660458.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LRB02_002287"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="LRB02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL4660459.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="LRB02_002288"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="LRB02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="LRB02_002289"
     CDS             complement(2948..4162)
                     /locus_tag="LRB02_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL4660460.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="LRB02_002290"
     CDS             complement(4503..4745)
                     /locus_tag="LRB02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4660461.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="LRB02_002291"
     CDS             complement(4777..5334)
                     /locus_tag="LRB02_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4660462.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="LRB02_002292"
     CDS             complement(5347..5535)
                     /locus_tag="LRB02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL4660463.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LRB02_002293"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="LRB02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL4660464.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6531..6704
                     /locus_tag="LRB02_002294"
     CDS             6531..6704
                     /locus_tag="LRB02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660465.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7344..7817
                     /locus_tag="LRB02_002295"
     CDS             7344..7817
                     /locus_tag="LRB02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL4660466.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7826..8053
                     /locus_tag="LRB02_002296"
     CDS             7826..8053
                     /locus_tag="LRB02_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660467.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1685 c         1274 g         3010 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactcc
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     6421 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     6481 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     6541 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     6601 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     6661 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     6721 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     6781 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     6841 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     6901 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     6961 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7021 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7081 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7141 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     7201 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     7261 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     7321 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     7381 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     7441 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     7501 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     7561 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     7621 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     7681 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     7741 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     7801 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     7861 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     7921 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     7981 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8041 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8101 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     8161 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     8221 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     8281 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     8341 tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc
     8401 ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc
     8461 ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt
     8521 tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DAFFQY010000093         8555 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFFQY010000093 DAFFQY010000000
VERSION     DAFFQY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.093"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LRB02_002297"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LRB02_002297"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL4660468.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LRB02_002298"
     CDS             complement(1098..2201)
                     /locus_tag="LRB02_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL4660469.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LRB02_002299"
     CDS             complement(2291..3247)
                     /locus_tag="LRB02_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL4660470.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LRB02_002300"
     CDS             3528..5462
                     /locus_tag="LRB02_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4660471.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LRB02_002301"
     CDS             5919..6335
                     /locus_tag="LRB02_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL4660472.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LRB02_002302"
     CDS             6349..6915
                     /locus_tag="LRB02_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL4660473.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LRB02_002303"
     CDS             6938..7924
                     /locus_tag="LRB02_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL4660474.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="LRB02_002304"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="LRB02_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL4660475.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2517 a         1650 c         1737 g         2651 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DAFFQY010000094         8458 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFFQY010000094 DAFFQY010000000
VERSION     DAFFQY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.094"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            467..2104
                     /locus_tag="LRB02_002305"
     CDS             467..2104
                     /locus_tag="LRB02_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660476.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2249..2989)
                     /gene="truA"
                     /locus_tag="LRB02_002306"
     CDS             complement(2249..2989)
                     /gene="truA"
                     /locus_tag="LRB02_002306"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL4660477.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3180..3815)
                     /locus_tag="LRB02_002307"
     CDS             complement(3180..3815)
                     /locus_tag="LRB02_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL4660478.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3962..5203)
                     /gene="sstT"
                     /locus_tag="LRB02_002308"
     CDS             complement(3962..5203)
                     /gene="sstT"
                     /locus_tag="LRB02_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL4660479.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5401..6397
                     /locus_tag="LRB02_002309"
                     /pseudo
     CDS             5401..6397
                     /locus_tag="LRB02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6413..>8458
                     /locus_tag="LRB02_002310"
     CDS             6413..>8458
                     /locus_tag="LRB02_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL4660480.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIGSLSNDVGKEKS
                     "
BASE COUNT         2795 a         1550 c         1302 g         2811 t
ORIGIN      
        1 aatcagaatc gtacaaatat ttaataacca aatatataga acataactta atattaagtt
       61 atttgaaatt ccataaaaaa cgactgcagg aattattgta agaaaaggat aaaaatagtt
      121 cagtccatct ccataattgt taaatgttgt atagttgatt ggcccttcaa aaatagaaga
      181 taaccctttg attcgtgaaa gatgaaaaga taaatcacct gttgtataaa tttcttgagc
      241 atttctcata aaatttactg aatataaagt actaagtaag ataagaacta ctaaaagaaa
      301 tgctttttta cttattttca tagatttgtt gttctccttt tttaattcta ttttcagtat
      361 actagaattg tcttttttta ataagttttg cgttaaattt ttcatagcac tagtatgata
      421 tactagaaaa gaatcaattt ttattacttt taaataggag aaaaaaatga aaaaaaacaa
      481 aaaccaaata atagatatca tttttatgtt atttctaagt tttgcttacg tcatccctct
      541 attcatgtct atgggaatct atcatagtgt caatcaggat acttactttc atttatctcg
      601 aatcatagga ctagataatg tatggagttc tccagtaaac ttcaataact tcgatcatca
      661 tggaacaatg atgaatattt tttatccatg gcttactctt tatccagctt ttttatttta
      721 taaaatgatg ggaaatcttg ttttaagtta taatatctac tattttttta tcactttttt
      781 aacaatggtc gtttcttatt tttctatgaa acaaataaaa aacaatcgtt atatttcgct
      841 gttattttcg attatttata cattttccgc ctatcgtgcc attgacatat tccatcgtgc
      901 ttcattggga gaagctgtcg ctttaacatt tttgcctttg atactaatgg ggtgttacga
      961 aatttatatt agagattacc aaaaatggta ctggttgtct attggcatga cgctagtagt
     1021 gtatactcat ttactttctg tcgctatggt ttctgtattt attggaggaa cgttgtttct
     1081 ttctttttat ttttgggacc aaaaaatcgc tagactctta agtcttttaa aagctaccgt
     1141 cctcaccttt tttttaagtg cgggattctt aattcctttt attcaacaat ctagagcaca
     1201 agaattgaaa gtcccactgg gaaaagaatt aagtggtatg gctcctagtg atatgctgac
     1261 acacatttta aataataact ataacaacta tactattgga ttattcttat ttctcggatt
     1321 gattggtgct tttattttta taaaaaaatt aactgctgat gatttattta ttttctttct
     1381 tggtgtattt gttctttttt gttcaaccaa tttatttcct tggcaacttt ttaaccatac
     1441 accagttaag agtctacaat ttgtctggcg gttaaatggt ttttcttctt tatttatagc
     1501 ctatacgatg agtatagtga tctattatat tttttctact aaaaataatt catggaaaat
     1561 aatggtcttt acgtttcttg cactaatatt gcatctctca ggagtaagca attctatcca
     1621 tgctaacaaa gattcattaa cacttatagc acctgaagac gctgttgcta tagctgaaaa
     1681 ttacaatcat actgattatg caaacaaaga gtcaatctat catcctgata tgattaataa
     1741 tgatcaatat ttactaggta accaggaaat aaatccaaca actcatttta tgagtaacaa
     1801 acttacaatt gaattagata attcgaataa taaaaatact gtcttgacga cacctattta
     1861 tcgttataaa gggcaagtgg cttctattaa tggcaaacta gtacaaacaa aattatctaa
     1921 gtttggcaca actgaattaa ctattcctcc aggtataaat aaagtcgtta ttacttatca
     1981 gtacacaaaa ctagcaattg cttcaagata tttatctatc gttactttga ttttattttt
     2041 actttatcga tttacttttt ctaaacaaaa acaaccgaga agagaagtac aacattctca
     2101 ctagattttt atttaagaaa gactttttag ctgtacacat aaacaggcta aaaagtcttt
     2161 ttttatattt ctttaatttg cttcatacat tatttaacat cgatatttat actgtgaaac
     2221 atgcgtttca tccattatca aagtattttt atcgataata aacttcatcc aaaaacagac
     2281 cttgtgcagg gacggtttct cctgcatgtt gccgcacttt gctttcaaat atttcatcga
     2341 ttgtttctat cggcatggca cctgtgccga tttctagtaa tgtccccatg ataatccgca
     2401 ccattttata taagaatccg tctccgacaa aagtaaaatg aagcatttct ccctttcgtt
     2461 cgatagataa ttcatcaatc gtacgcactg tggatttttt tgattttttc aatgcagaaa
     2521 aaccaatgaa atcatgtgta cccactaatt tttcgcaagc tgcattcagc ttgtccatat
     2581 ccaactcttg agggaaataa aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt
     2641 tccaaacata atagctgtat tttttgccca ccgcattata tcttgaatgg aagcgctcag
     2701 gcatttcttc gattcttttg atgacaatat cccgcggaag gtaacgattc ataaagtcga
     2761 tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat
     2821 gggtgccagc atctgttctt cctgatccaa taatttctat ctcttcattt gtcatctgat
     2881 gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc atctccaagt ctttgccatc
     2941 ctgaatatct ttttccgtca tattcgattg tcaacttgat gtttctcata ttttagtggc
     3001 tcctctgtct tccatttggt cttctctcta cctttttaag attgtagcac aaaatatagc
     3061 tcctttcttc tctaaaacag aagctataca agaaaactgt ggaaatatat atttcagaaa
     3121 aacaaaaaac agccaccgga tgaagtatcc gctgactgtt caatttgacg gctcaagttt
     3181 tactgtttga gatgcaagat actcaaccag gcaatgggca cataattaaa gaaaaaactg
     3241 attctttggc aaaaacctat tttattcagt aatggccaag aatttataga aggaattctt
     3301 gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag acagtccaag gtataagtgg
     3361 cttacgtcca tttgcccatt ttttcgatag atcaagaaaa gaagaactgg aaacaataaa
     3421 aaaccactgt aacctaaacc tgatccgata ttatggaccc atgtcgagac attccacgaa
     3481 gattcatggg tattgatact gaacaaacct gtaaagatac aatcacctat accatagaac
     3541 gcgatggctg ctgtaactag ggtagctaac acacgagatg tacttacaaa tgcagcatac
     3601 aatgcaggca atgacaggat gaaaaagatc cctgaaacca cagaccaaat aagaaacgct
     3661 tttcttacag gactggaaac ctctccaaat acactgatca cgctcttcat ttgatctaat
     3721 tcaggataat acaatcctaa tacgtacgga gtcaaaaaat cactcagcac gcctaataat
     3781 aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac gcctccaata acaattacct
     3841 atgcttttaa ttgttattat aagcgacttt atcttgattt aacagaaacc tctatttaca
     3901 cgaacatcta tataactcat taaaaactcg aatcatttta aaaatactaa atatctaacg
     3961 ttcaacgttc acttaatcca tctaaacaat ctttcaattt gattttttcc ccacttcttt
     4021 tacgcgcgcc tagctctcct attgcagtaa acaacagatc acttgacgaa tttaaggcag
     4081 tttccattga atcctggata acaccgataa taaatccaat accgacgatt tgcatcgcga
     4141 catcattaga gataccaaac aaactacatg ctaatggaat caataacagt gaaccgcccg
     4201 caacacctga agctccacaa gcagctaaag cagacaaaat acataaaagg actttcaaaa
     4261 caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa
     4321 tcgcggcacc gcccatattg atcgtagcac ctaacgggat cgatatggaa taagactctt
     4381 ttgtcaattt caatctttca gccaacatca tgtttatcgg gatatttgcg gcagaacttc
     4441 gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat
     4501 tttctttaat aaacaaaaac gtgaggaatg ggtacacaac taatgcgaca aacagcatcg
     4561 taccgatcaa aacaagtaaa agctgcagat actctgccaa tccagctatt ccagtcgtag
     4621 caattgattg ataaaccaat cctaaaatcc ctatcggagc aaatgcaata atcacttgta
     4681 cgacttgtga caacgctata gatatctgat ccaccaactc ttttgtcgat tctgaacgtg
     4741 ctcgaaaagc tagtcccaac aatacagccc agaataagac gccaaggtaa ttcgcatcaa
     4801 taagagattg aacaggattt tgtgtgacat tcgtcaacat agtagataaa acactttcca
     4861 aactttgcgg tgctttttca gatactgttt cttgtaaagt aataggaaca gtaaaaattg
     4921 aacttacaat aactgctaca agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa
     4981 taggcttcac gaatgtctta ttcccaattt tatgcttggc aattgacgcc atcgtcaaga
     5041 aaaaaaccag aataggtgcg attgccttca agcaactgac aaacaacgtt cccaatattg
     5101 aaataaatgt ccatgtgggg aaaaagatac caaacaatat tccgactaaa ataccaacaa
     5161 caatacgttg aactaacgac atatccaaaa acttctctaa caaatgacca ccctttcaca
     5221 attttaacaa tcattgtatc attttatgca aagaaactaa aggtataatt ttctaaaaat
     5281 tctaacaata caaacaacca tttcttgcta attttgtact ttttctgtct acacgttgtt
     5341 ttttgagata ttctaagtta ggatgttatt aatgggcaac ttatcaggaa acgttttctt
     5401 atgtttttgt ccaatttttt tgtgaggtgt ttattcttga aaaaaataaa gaaggagagt
     5461 tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg ttttacagcg tatattgatt
     5521 tcagcagtca ctattcctac atatgcaaca aaccctggtc gattctacat ctatacattt
     5581 ttacaaactt ccgttgttat tggtgcaaat ttgatcccac tttacatggg ttaccaatat
     5641 caaaaaatcg cccgtttaaa agttttacat tatttaagtc gttttgcctt tatattccta
     5701 atagccacac tagtttgcaa tatctttttt ttacgtacaa gctggcagct tgaatattcg
     5761 tgattattgg cttatcctaa cacctatcag tcagaattac tttacttatg ctgtttcttg
     5821 tactttgctt ttttgtagta ttccttatac agttggattt ttaaataaat tatcgaaaac
     5881 aacaattaaa tatctactat tatttttaac aattggatta gttggctgct ctactctatt
     5941 taataaagac ccgtttgcat tacagaatgg acagagtatt ttatggattt tttatttatt
     6001 tttaatgggt tatggtttga aagaatggaa ctggaaagaa aaggtccgct ataagcttcc
     6061 tcaagtattt atcagcttga ttgttttatt cggtttgatt attttgatga cacaaatttc
     6121 actcatcatc agaggaaata cagatacagc gctgaggttt tctaaaccat attctctctt
     6181 cgcaatgtat tatactttct cttcgttttt atttttagaa atgctatctg ataaattagg
     6241 tataaaagga agaggaaatt ttcttgctgt atttttgatt gtcactcaag ttatatgtaa
     6301 tttcccttta gtttcttatc gcgtatctac atttatgaaa cgtgaatttc caaactcagg
     6361 gaaggcatgg ctgataaata tcgggcagtt ttttatttgc tatttattag cagtgtccct
     6421 cctagttatc tttttatttt atatacaaaa aatccgtttc attcatcgtt tcatcgaata
     6481 cttcgctttt gattcgattg aacaattaac tcagcgttgg aaaaaaagaa ttcattggct
     6541 ttttgttcat aaaagttatt ttttagttgc agcatttttt tactgcttta ccttcgtgca
     6601 gattttcgtt ttagaaccaa aagaaggttg gaaagaaact atccaggttg ccttaaaaat
     6661 atttactcag cgacaagcac ccatgatcct gacaatcatc attattatgg gatttttctt
     6721 cttactattg ttgatttcca atcgtttttg gtacgccttg actgctacct tgatcattaa
     6781 cttactgctc actatttcga cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc
     6841 ttcagattta aaaatactga ccggattgtc agaactatta tcgatggtta gcccagtctt
     6901 attaatcgtg ggcggattga tccttctcct attattgatt actagtatta ttgtacaacg
     6961 tagactacaa aaacagtatt ccttgaagat tcattggaaa cgtcgattta tcagtatcgc
     7021 tgtattatta ggatgttttt cgggtgtctt tttcattaat cacaaaaatt cgccttcttt
     7081 cttgctgttt aatttattta aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa
     7141 agataacgga ccagtcattc aattcctaaa caatatcgac attaaaatca tggaagaacc
     7201 tgctggctat tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca caaaggcaga
     7261 tgaaatcaac caaacaagaa gtgactggtt aaacaatcag acgatcgttc tcaatttaag
     7321 tgaaagcttc agtgatcctg cacgtgtccc taatctgaca gtacctacag accctattcc
     7381 ttacatcaaa agcattataa cagaaggaac aggaggattg atgttatccg tcggttatgg
     7441 tggcggaaca gctaacatgg aatgggaagg actgacaggc ttgagtatca gtaacctgtc
     7501 tgcttcgtta gttacacctt atacccagct agtggagcgt cagaaaataa gtccaaatat
     7561 taccgatcta ttcgatgaaa aaatagccat ccatcctttt acagctgctc tttatcgcag
     7621 aaaacaagtg tttgataaat ttggttttga caggttctat tatttagaca gtccggataa
     7681 attaacttat acggataaaa tagaacaaag cccacgcata tcggatgatt cagcatacaa
     7741 agaaaccttg aaagctttac aaagcaatac agaaacgtca caattcatcc aattatccac
     7801 tatgcaaaac catatgcctt atgatgatta ctatagtgaa ttgaactaca ctgctgaagg
     7861 aactgctgtc attcctaata gaagaaaaga attacaaact tatatgcaag gccttcacta
     7921 taccgataca gcagtaaaaa aattcatcca agaaatcgac aaaatcgata aaccgattac
     7981 ctttgttttt tatggtgacc atttacccgc aatctatagt gggaatgatt cgaaaaaata
     8041 tggattagaa cagcaccaga cagattattt catttataac aacaagtata gtcgtgaaca
     8101 agcagctacg ttaaataaag aagttgttgc tcctaataac tttggtgcta tggctcttga
     8161 gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg acagaagtat ctaatcatct
     8221 acctgcatta acaattgatc ctacagaaag cttgtctaat cgttttaatg gaaaacaggt
     8281 ctttgttaca gaggatagtc aaattatcca agaaaaaaat ttaactgaga aacaaaaaga
     8341 aattctagaa gattatcgtc tgatacaata tgatcttgtt gcaggtaacc agtacagtgc
     8401 aacatgggcg acacaaaaaa taggctcttt gtcaaatgat gttggtaaag agaaatct
//
LOCUS       DAFFQY010000095         8288 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFFQY010000095 DAFFQY010000000
VERSION     DAFFQY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.095"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     regulatory      140..219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            217..1410
                     /locus_tag="LRB02_002311"
     CDS             217..1410
                     /locus_tag="LRB02_002311"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL4660481.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1540..3012
                     /locus_tag="LRB02_002312"
     CDS             1540..3012
                     /locus_tag="LRB02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4660482.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3095..3883
                     /locus_tag="LRB02_002313"
     CDS             3095..3883
                     /locus_tag="LRB02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL4660483.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3950..4618)
                     /locus_tag="LRB02_002314"
     CDS             complement(3950..4618)
                     /locus_tag="LRB02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL4660484.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4733..6445
                     /locus_tag="LRB02_002315"
     CDS             4733..6445
                     /locus_tag="LRB02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4660485.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6448..8220
                     /locus_tag="LRB02_002316"
     CDS             6448..8220
                     /locus_tag="LRB02_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4660486.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2538 a         1387 c         1821 g         2542 t
ORIGIN      
        1 tttaaaaagc ttagcagagt tatctttatg gatgattgtg ctaagctttt ttggacagtt
       61 ctaatgtaaa atatttttat atttttaagc aaaaaaatga aaaaatacga gcagaaaact
      121 gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg
      181 ccttgtaacc gaatttaact agggggaatc attataatgg tagaaagaca cttatttaca
      241 tcagaatctg tttctgaagg acatccagat aaaattgctg accaaatcag tgatgcaatc
      301 ttggatgcaa ttttaaaaca agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca
      361 actggtcttg ttttagtatt tggagaaata tctacgacgg catatgtaga tattcaaaaa
      421 gtcgtacggg aaacaataaa agaaattggt tatacacgtg caaaattcgg atttgatgga
      481 gatacagcag ctgtattggt tgcgattgat gaacaatctc ctgatattgc tcaaggagtt
      541 gatgaagcac ttgagatccg ggatgaagat aaaaaagacg tattagatga gattggtgct
      601 ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa caccagaatt gatgccttta
      661 ccaatcgctt tgagtcatcg tctggtacga cgcttggcag atttgcgtaa atcaaatgaa
      721 ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg ttgaatatga tgatcaagga
      781 caaccggaac gcgtagatac aatcgttatt tcaacacagc atgatgatgc agtagataat
      841 gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg aagttattcc agctgaatta
      901 ttagatgatc aaacgaaata ttatatcaat ccgactggcc gatttgtcat tggtggtcct
      961 caaggggatg ccggattaac aggaagaaaa atcattgttg atacgtatgg cggttatgct
     1021 cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc
     1081 tatgctgcac gttatattgc taaaaacatt gttgcagcag ggcttgctcg caaagcagaa
     1141 gtacagctag cttatgcgat tggtgttgct caacctgttt cgatctcaat caatactttc
     1201 ggtactggaa cggttccgga agaagaactg attgctgcag tgagagaaaa cttcgatctt
     1261 cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct
     1321 gcttacggtc attttggtcg tacagatgta gatttgcctt gggaacatac ggacaaagta
     1381 gatgcgttga aagcaagttt agcaaaataa gttatattca gctggaacga tcgccaagtg
     1441 gtggttgttc cagctttttt tatggcagtt aacctcttga aaaagatagt aattatttat
     1501 ctatttttag atcctgtcat aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt
     1561 actttggtca cagtgagtat tttcattgca acgttcatga cggctattga aggtacaatc
     1621 gtaacaaccg ctatgccaac gattgtcggt tcattacatg gaatggaaat catgaactgg
     1681 gtattttcga tttatttgct gacaaacgct atgcttactc cgatttacgg aaaacttgcg
     1741 gataaagtgg gacggaaacc aatctttatt tttgggacat tattgtttat tatcggttcg
     1801 ttattttgcg gtttatctca aagcatgatt gggttgattg ctgctcgtgc gattcaaggg
     1861 atgggtgctg gggcaatgat gccagtagct ttgacgatca tcggcgatct ttattctatt
     1921 gaaaagcgag caaaagtttt aggtttgaat agttcagcct gggggattgc cagtgttttt
     1981 ggaccgcttg cgggaggatt catcgttgaa acgatcagct ggcactggat cttttttatc
     2041 aatgtgccga tcggaattct attaattatt ttgatttggc tatatttaaa tgaaccaaaa
     2101 atcatacatg agtctaaacc aatggatatt tttggtagtt ttagtttgat ggccatgctt
     2161 gtctcattgc tgttagggtt ccaattatta ggagacgaag gaatgagtcc gcttgtttct
     2221 gctttgctgc taggtagtat cgtaatgtta ggtatttttg tttttgccga aaaaagagct
     2281 aaagatcctg tcatcatgat gcatttattc aaaaatccac tatttgttgt ggtcaacttg
     2341 gctgcagcat tggctagtgg ttttttgatg ggaatcgatg tttatatccc tatgtggatg
     2401 caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg tattagcacc attatcacta
     2461 gtctggatgg tcggttcttt ccttgcaagc aagtggatga ctcaaatgaa aactcattgg
     2521 gtcttgagaa ttggactact aatcgcagct attgggggcg cttggttgta tcttatgcct
     2581 ttcagaagta actatttatg gttttttgct atatcaacgg tcatcggtat aggcttaggt
     2641 gtgaccatga ctacctcgac tgtcagtgca caaaatagtg tatctcgtga ccagcttggt
     2701 gtagcaactt cttttaatac gttagttcga acaattggtc aaacagtcat ggtttctatt
     2761 tttggtttat tgataaatgg ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat
     2821 gatgcagata tcatgaataa actggtgaat ccacaaacgg ctaaatatat tgatgcggat
     2881 attttgttac cattaagaaa aattctttat gatgggttac attccgttta tctagttggc
     2941 ttgttactcg tgatcattgc tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa
     3001 ataaaacagt agtttcggtt aagatatgta cgtagccgaa tcattcaaat ttaaagttat
     3061 tcaaaaataa cttcttacta aggaaggcga aaatatggca tttattcaag caaatattta
     3121 ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa
     3181 ggtgattggc accagttcag agcaagagac aacggatata cctgttttat atttacttca
     3241 tggaatgggt ggcaatcata gtgtatggga aagacgtacg tcgattgaac gttatgtagc
     3301 aaactacgga ttagctgtca tcatgccatc tactgatctt ggctggtaca cagatacagt
     3361 ttatgacatg aagtattgga cattcattgc agaagagcta cctgagattt gtcatgaatt
     3421 attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc gctggattat cgatgggcgg
     3481 atatggcgcg ttaaaactag gccttgcaaa atcagaaaat ttcgcagcag taatctctct
     3541 ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc
     3601 tttttgggaa gggatctttg gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca
     3661 atacttattg aaagaactag taaaaagcgg aaaaccagtt ccaagatttt atatggcttg
     3721 tggggaatct gattttcttt atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca
     3781 aggcttggcg gtcacctttg aacaaggacc tggcgatcat aactggatct tctgggacca
     3841 atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa
     3901 gatagctcct atagagatca caaacatagt gatctctata ggagcttttt tactgctcgt
     3961 tttctcttct tttattttgt tggcataagc cttcaatata ggtttgtgca ctcgttagaa
     4021 agagtgcttt ttcattcaat tctgcatggg actgcatact ttctttaaat ccctctaatt
     4081 gattttcaac tacataatta cgcataaata gaactgtttc tttcaaaaat tcatttccgt
     4141 ccataatttt ttgtcgaagc tgctgttcat gctgctgatc cattaactgt ccaatcaata
     4201 aattgtggag agaggcaatg gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa
     4261 gtaattggat catttgctcc aaatgttcta atcgactcgg aaaagcgggc acagtattga
     4321 tttctaattc tgccccacaa gggaaacgtg tataaaggtc atagtaattt tccatatact
     4381 gcagcaactg ttcattccaa ggcaaagttg gatctggaag cgctaattct tcttccattg
     4441 tttcagccat tttttgcaag agctgttgtt tgtttttgaa ataccaatat aaagcaggtg
     4501 cttgaacatg aattctgttt gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt
     4561 cagccaggat atcaaaggcg gcctgaatga tgattgcttg agaaagcttt atttccattt
     4621 tatcaacctc gtgtattgca ttatcttttt aaacatggta aattgttagt cggtctaatt
     4681 ttacagtgtt aaattagtcc tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt
     4741 aatcttacga catgcgaaaa aatataaagt agctgttttt atttccttgc tgtccgtagc
     4801 agtcatggtt gcagcagcat tatggcaacc taagttacta cagcaagtat tggaagctat
     4861 tattacagaa gataatgatg aaatgaaaac aattggtatt tacctgatca gtctggcttt
     4921 gctaggatta gccgcaggtg taacgaacac gattttctct gcaaaagtag ctcaaggcgt
     4981 aagtgcagat atccgagaag aagcatttcg aaagatccag acattttcat ttggaaatat
     5041 cgaacagttc tctgctggaa atcttgttgt tcgtctaaca aatgatatca cgcaaatcca
     5101 aaatttagtg atgatttctt tgcaatcact ttttcgaatt ccattcttat ttattggtgc
     5161 cttcatttta gctatgatca cgatgccgca attatggtgg atcatcgtag cgcttatcgt
     5221 tacggtattt ctgattacgg cactttcatt tacaaggatg ggcaagcact tcatgatcat
     5281 tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa aacttactag ggattcgtgt
     5341 agtgaaatct tttgtacaag aagaaaacca attgaagtca ttcagcaaag taagtgagga
     5401 cttaacaaaa cacaacatca tagtcggtac attattttct gtgatgatcc cgtccttcat
     5461 gctggctgcc aatttggctg tggttggggc gatctttttt gtgagcgatc ttgttaaaga
     5521 tgatcctacg ttaattggtg gaattgcttc attcatgaac tacttgatgc agattatgat
     5581 ggcaatcatt attggcggaa tgatgatgat gatgacttca agagcagccg tatcgctgaa
     5641 gcggattgcg gaaattttag atgctgaacc agatcttact tatttagacg ttccagaaca
     5701 agagttgacg ggttctgtca cttttgacca tgtttctttt cgttatcctg gtgaggatac
     5761 agatacgctt aaagatatca gcttttctat caaacctgga gaaatggttg gtatcgtcgg
     5821 ggcgactggt gccgggaagt caacactagc acaattgatc cctcgtctat ttgatccgac
     5881 agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag
     5941 acaaacggtt tcctttgttt tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa
     6001 cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg gaacatgctt cttctattgc
     6061 tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac gaagcaccaa tagaagaacg
     6121 tagcagtaac ttttctggtg gtcaaaagca acgtttatcc attactcgtg gcgttatcgg
     6181 taatccaaag atcttgatcc tagatgacag tacgagtgca ctggatgctc gctctgaaaa
     6241 gctggtccga gaagcactcg atcgagattt gaagggaaca acgacgattg tcatcgcaca
     6301 aaaaattgct tctgttgtca aagcagaccg catcttagta ttggatgaag gcagattagt
     6361 cggcgaagga acccatgagg aattggtagc tggaaaccgt gtctatcagg aaatctttga
     6421 aactcaaaaa ggaacggagg agtaagtatg actgatctaa taaaagcaag taaatttttc
     6481 tatcactatt tgaagcggta caaactctcc tttttcttta tctttatcac agtgatcatt
     6541 gctacctatc tacaagtaaa ggcacctcaa tacatcgggg aagcttttca agaattagct
     6601 aattacgtag gaggcttgat gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata
     6661 tggaaactct tgctgtttta tgtactagct agtgcagcca actttatcta tagtattttg
     6721 ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta tcggtctatt caacaaattg
     6781 gaaaaattga cgatccgttt ctttgattct catcaagacg gtgaaatttt aagtcgtttt
     6841 accagtgact tagataatat tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg
     6901 aatgctgttt tacttgtcgg gatcttgatc atgatgttcc gccaaaatgt agagttagct
     6961 tgggctacaa ttgcttccac tccagtagcc gttttgatag ctgtcttgat tatccgcaaa
     7021 gcacggaaat atgtggatct tcagcaagat gaagtgggga aactaaatgg ttatatggat
     7081 gaaaaaatca gcggacaacg tgtcatcatc acaaatggct tacaagaaga aacgattgaa
     7141 ggattcttag agcataatca aagtgttcgc gaagcaacct tcaaaggaca agcttattct
     7201 ggtctactat ttccgatgat gcaaggaatg tcactggtca atacagcgat agttatcttt
     7261 ttcggcggct ggttagcatt aaatggtgat ttggaacgtt cggtggcatt aggcttagtt
     7321 gtgacattcg tccagtattc acaacaatat taccaaccac tgatgcagat ttcatctgga
     7381 tacagcatga tccaattagc gattacaggg gcaagacgat tgaatgaaat atttgatgaa
     7441 aaagatgaag tcaaaccaga aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg
     7501 ttgcagcacg tagattttgg ttattctcca gaaaaactag tcttgaaaga tgtctcgatc
     7561 aatgtggaca aaggagaaat ggttgctctt gttggtccaa caggatcggg gaaaacgacg
     7621 atcatgaatc tgctaaaccg tttttacgat gtcaatgatg gttcagtagt catcgatgga
     7681 atagatatcc gagattatga tcttgatgca ttacgtagac atgtaggaat cgttttgcaa
     7741 gaatctgtat tgttctccgg aacgatcaaa gacaatatca cattcggcaa accggacgct
     7801 tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata ttcatgactt catcatgacg
     7861 ttagaaaaag gttatgatac agagatatcc gaagaaaata acttattcag tacaggtcaa
     7921 aagcagttga tcagtattgc tcgaacgatc atcacaaatc catctttatt gattttggat
     7981 gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga tccaaaaagc aatggatgaa
     8041 gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca
     8101 gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag aaggcagtca tcatgagtta
     8161 ttagcagaaa atggatttta tgcggaactt tatcataatc aatttgtatt tgaaagttaa
     8221 attaaaagaa cctcgttata acggggttct ttttatatat tttatgttgt ttgtttttat
     8281 aaggggca
//
LOCUS       DAFFQY010000096         8185 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFFQY010000096 DAFFQY010000000
VERSION     DAFFQY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.096"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(233..1081)
                     /locus_tag="LRB02_002317"
     CDS             complement(233..1081)
                     /locus_tag="LRB02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4660487.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="LRB02_002318"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="LRB02_002318"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL4660488.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="LRB02_002319"
     CDS             complement(3582..3761)
                     /locus_tag="LRB02_002319"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL4660489.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="LRB02_002320"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="LRB02_002320"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL4660490.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..>8185
                     /locus_tag="LRB02_002321"
     CDS             5909..>8185
                     /locus_tag="LRB02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4660491.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2410 a         1728 c         1471 g         2576 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactg
//
LOCUS       DAFFQY010000097         7980 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFFQY010000097 DAFFQY010000000
VERSION     DAFFQY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.097"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="LRB02_002322"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="LRB02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL4660492.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="LRB02_002323"
     CDS             1229..2332
                     /locus_tag="LRB02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4660493.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="LRB02_002324"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="LRB02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL4660494.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6269)
                     /locus_tag="LRB02_002325"
     CDS             complement(3822..6269)
                     /locus_tag="LRB02_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL4660495.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6458..7894)
                     /locus_tag="LRB02_002326"
     CDS             complement(6458..7894)
                     /locus_tag="LRB02_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4660496.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2214 a         1736 c         1359 g         2671 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgtttctt ttgtgccatt tattatccct catttcatca agtttcatta
     6301 tacaaaaaaa agtaagggaa ctaaagtttt gtttgaaaga actgacgctt tctttcattt
     6361 ttcagcttat tttacagttt ttcttaattt ctaaatgatt ttatcaagaa aaggcacggc
     6421 catatccagc cgtaccttct tgttttaagt atgtgaacta aaggttctct ccattttctt
     6481 caatcagttt ttggtaccag taataactgt ctttcttata tcgttccaaa gatccttctt
     6541 gtgtttcgtc ttgatcaaca tatacaaatc catatcgctt ttgataaccg ttcagccagc
     6601 taagcaaatc tgtataagac caggtgcaat agcctaggac ttctgctcca tccgaaattg
     6661 cttctttgat ggctttgacg tggctttgta agtaattgat tcgatagtcg tcatggattt
     6721 gtttatcttc tgtcaacttg tcatactctc ctaagccatt ttccgtaatc agtaccggca
     6781 tgcggtaacg gcttgtgata cgacgcagtc cgatccttaa tccttctggg tcaatctccc
     6841 agtcccaatt tgttcgttcg acaaatggat tctcaacttt tttatacatt ccaggaactc
     6901 cagtttcttc tgatgaacct ttttttcctg tcgtattcat ttttccagca ccaactccgt
     6961 ttaatggatt gaatgcattc gtcgccgttt gatagtaatt gatcccaaga aaatctggtt
     7021 tagcagattc tagcaattct gcatcgccag cttcaatcgt tggtgcccat cctttttctt
     7081 ctaagaacct catggcagca atcggatatt ttccccacat atagacatcc atccaataat
     7141 tggccatcag gtcttctgca ttttcagcag ccagaacatt ttcaggatta ctgtcaattg
     7201 gataatttgg cgtatacgca aagcttggtc cgatttttcc aggcatttct aactcgtgaa
     7261 atttattgat gacagatgca tttgctaaat tagcaatatg gttaacttca tacattcgtt
     7321 tcgggtctgt cacacctggc ggatgtgctg ctaacaaata acctaatgaa gtgaagatat
     7381 tttgttcatt caaactaatc cagtaatgga ctttcccgcg gaatgcctca aataatgttt
     7441 ctgcataatg agtgaaatct tcaatgatct gacgtgattc ccaaccatta tattcatctt
     7501 gcaatgcttg tggaagatcc caatgatata aagtcaacat aggttcaata tcgttttcaa
     7561 tcaattcgtc gatcaatctc tcataaaaag cgagcccagc ttgattgact tctcctcgtc
     7621 cattaggaaa aatccgtgtc caagctacag agaagcggta agctttcaga ccttgttctt
     7681 tcattaaagc aatatcttct ttataacgat gatagtggtc tactgccaaa tcaccgttcg
     7741 ttcctttaaa tgtctttcca ggtatccgta caaatcgatc ccagactgat tctcctttcc
     7801 catcttcttg ccaagctcct tctacttgat acgctgcaga agcagagccc cacagaaaat
     7861 cttttggaaa tgaggtaagt tctttgtgtt ccattcatct cttcctttcc tataaccttc
     7921 aatcgttcct cttttagaat aacacgaaac cccttccaca acaaaataaa attatgtaaa
//
LOCUS       DAFFQY010000098         7718 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFFQY010000098 DAFFQY010000000
VERSION     DAFFQY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.098"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="LRB02_002327"
     CDS             168..362
                     /locus_tag="LRB02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660497.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LRB02_002328"
     CDS             631..930
                     /locus_tag="LRB02_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660498.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LRB02_002329"
     CDS             complement(1027..1461)
                     /locus_tag="LRB02_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660499.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LRB02_002330"
     CDS             complement(1693..2403)
                     /locus_tag="LRB02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL4660500.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LRB02_002331"
     CDS             complement(2393..3253)
                     /locus_tag="LRB02_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL4660501.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LRB02_002332"
     CDS             complement(3258..3896)
                     /locus_tag="LRB02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL4660502.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LRB02_002333"
     CDS             complement(3911..4210)
                     /locus_tag="LRB02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4660503.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LRB02_002334"
     CDS             complement(4253..5737)
                     /locus_tag="LRB02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL4660504.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LRB02_002335"
     CDS             complement(5757..6218)
                     /locus_tag="LRB02_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4660505.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LRB02_002336"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LRB02_002336"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL4660506.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..>7718
                     /locus_tag="LRB02_002337"
     CDS             7521..>7718
                     /locus_tag="LRB02_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4660507.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYK"
BASE COUNT         2370 a         1619 c         1220 g         2509 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaa
//
LOCUS       DAFFQY010000099         7623 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFFQY010000099 DAFFQY010000000
VERSION     DAFFQY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.099"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LRB02_002338"
     CDS             complement(14..706)
                     /locus_tag="LRB02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660508.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LRB02_002339"
     CDS             complement(1143..1712)
                     /locus_tag="LRB02_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4660509.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LRB02_002340"
     CDS             complement(1786..2109)
                     /locus_tag="LRB02_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660510.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LRB02_002341"
     CDS             complement(2263..3075)
                     /locus_tag="LRB02_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660511.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LRB02_002342"
     CDS             3429..4277
                     /locus_tag="LRB02_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL4660512.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LRB02_002343"
     CDS             4422..5363
                     /locus_tag="LRB02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL4660513.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LRB02_002344"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LRB02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAFFQY010000100         7595 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFFQY010000100 DAFFQY010000000
VERSION     DAFFQY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.100"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(940..1353)
                     /locus_tag="LRB02_002345"
     CDS             complement(940..1353)
                     /locus_tag="LRB02_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL4660514.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="LRB02_002346"
     CDS             complement(1809..2294)
                     /locus_tag="LRB02_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660515.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="LRB02_002347"
     CDS             complement(2433..2678)
                     /locus_tag="LRB02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL4660516.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="LRB02_002348"
     CDS             2871..3671
                     /locus_tag="LRB02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4660517.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5557
                     /locus_tag="LRB02_002349"
                     /pseudo
     CDS             3690..5557
                     /locus_tag="LRB02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            5550..6041
                     /locus_tag="LRB02_002350"
     CDS             5550..6041
                     /locus_tag="LRB02_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBL4660518.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDGHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6029..6583
                     /gene="srlA"
                     /locus_tag="LRB02_002351"
     CDS             6029..6583
                     /gene="srlA"
                     /locus_tag="LRB02_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL4660519.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6601..>7595
                     /locus_tag="LRB02_002352"
     CDS             6601..>7595
                     /locus_tag="LRB02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL4660520.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2705 a         1214 c         1443 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaagcgatt
     5101 agccgggttt cattctactg tttttttcta tcaaatatgg aaagaaaaaa agacgtatca
     5161 agaatattta aacaaattag tcgctccttt gatcagaagc tattatgtgg atgaagaatt
     5221 tgaaaaaagg atcttgaaaa gagaatatca gcaaagcaca tttgttaaca aagaatttgc
     5281 ttttcctcac acaaccaacc agctatctca acacattgtt ttatcagtag ggctaataac
     5341 tgatcaaggt ttagcagaaa caggaggaaa tggagttatt tttttatttg ccatacctga
     5401 agaaatgacg gctgaagttg agaatgaatt atttgagcta tacgatatcc tatttgaact
     5461 cataaaaatg ggaatagatg agtttattga attagcaccg acacaagaat catttttaaa
     5521 actaatcaaa agtaaaggag agagacttca tgaatgatat gtatctattc atagttttcg
     5581 cagcttcagc ttatgtgctg caagcagtta tcagttggaa gcaactaaag catttcaatc
     5641 aaacatatag cgaactgcga agaaaaggaa aagtagcgat tggtaaacga cctgggaaaa
     5701 tccgttcagg cacgattgtt atgctagcta tagacggtca tgatcaagtt ttagaagtca
     5761 gaaaaatgca aggggttaca tctttggcaa aatttcattc attacctgtc tatgcgggtg
     5821 aagatatcca ttacatcgat cgttaccatc cctcagttcg agcggaagat cgtttgacac
     5881 aagcagcttt agaaaatgcc agagaagtat acttacgaat agaggcagga aatgaaaaaa
     5941 gtgaacaacc ttcattgcct tttattgcta tgctcaataa ttttcggata tggaaaagta
     6001 cgatcaaaga aaaaaggagt gacaaacgat ggatatcata acaaaatttg ctgagggatt
     6061 tatgaggctg ttccaaacag gtgcagaaac attcataagc tggatgacag gaatcgtacc
     6121 agttgtcttg atgctgatgg ttgcaatgaa tacgctgatt gcttttttag gtgaagaccg
     6181 agtaacgaaa gtagccaaat tatcagcgaa aaatccgctt actcgttacc ttgtgttacc
     6241 gttcttatct gcttttatgt tgggaaatcc tatgtcattt acaatggctc gtttccttcc
     6301 agaatactac aaaccaagct attatgctgc tcaagctcaa ttttgtcata cgagcaatgg
     6361 tgtattcccc catattaatc ctggagaatt gtttgtatgg atggggattg ctcaagggat
     6421 cgaaacctta gggctaaatt cgatggagct ggctatccga tacttgcttg tgggactgct
     6481 gatgaacttt attggtggtt ggataactga ttttactact ggatttgtat gcagacagca
     6541 aggaatcgtt cttagcaaaa aagtagagct ttcagttgat tagagaaagg agagttttaa
     6601 atgtatggtg atattaagat cgtaagagga aacggcgggt atggaggtcc tttgattatt
     6661 aggccgacaa aagataaaca caagttcatc tatgtcacag gtggaggtga gaggccaatg
     6721 atcgtagata aaatagaaga actgacaggt atggagtcta tcaatgggtt caaaacatca
     6781 atacctgata gtgaaatcgc gcttgcaatc gttgactgtg gagggactct tagatgtgga
     6841 atttatccta aaaaagggat accaaccatc aatatcgttc ccacaggaaa aagtggccca
     6901 ttagcacaat atatcactga agatatttat gtttcagcag tagggttaga acaaatacag
     6961 ccactaatgg aaactgatga taacaaagaa acagtaaata ctgcatctcc agataaaaag
     7021 tacaaatatg atacttctaa aaagataacc gagcaaaaag cagagcagca aaactggata
     7081 gcaaaaattg gaatgggcgc gggaaaagta gttgctacat tcaatcaatc agcaagagaa
     7141 gcagtagata cgatgatccg gacagtgatc ccttttatgg ctttcgtttc ccttttgatt
     7201 ggtgtgatac aaggttctgg gatcggtaat gtatttgcta acattatggc acctttagca
     7261 ggaaatgcag ttggattaac aatcattggc tttatttgtt cactgccatt tttatctcct
     7321 ttgcttggtc caggggcagt tattagccaa gtgattggaa cattgatcgg tgtagagatt
     7381 gggaaaggaa atattcctcc ccaattagca ttaccggcac tatttgctat caatactcaa
     7441 aatgcatgtg attttattcc agtcggtcta ggtttggaag aagcaaaagc tgagacagta
     7501 gaagtaggtg taccatcagt tctttattca agatttctta acggcatccc acgagttatc
     7561 attgcttggg tagcaagttt tggattatat agctg
//
LOCUS       DAFFQY010000101         7187 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFFQY010000101 DAFFQY010000000
VERSION     DAFFQY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.101"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LRB02_002353"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LRB02_002353"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL4660521.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LRB02_002354"
     CDS             complement(1172..1867)
                     /locus_tag="LRB02_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL4660522.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LRB02_002355"
     CDS             complement(1880..2596)
                     /locus_tag="LRB02_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL4660523.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="LRB02_002356"
     CDS             2926..3534
                     /locus_tag="LRB02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL4660524.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="LRB02_002357"
     CDS             3859..4182
                     /locus_tag="LRB02_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL4660525.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="LRB02_002358"
     CDS             4364..5734
                     /locus_tag="LRB02_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL4660526.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="LRB02_002359"
     CDS             6081..>7187
                     /locus_tag="LRB02_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4660527.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DAFFQY010000102         6635 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFFQY010000102 DAFFQY010000000
VERSION     DAFFQY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.102"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            99..746
                     /locus_tag="LRB02_002360"
     CDS             99..746
                     /locus_tag="LRB02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL4660528.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LRB02_002361"
     CDS             1139..3784
                     /locus_tag="LRB02_002361"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL4660529.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LRB02_002362"
     CDS             4094..5407
                     /locus_tag="LRB02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL4660530.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LRB02_002363"
     CDS             5397..6050
                     /locus_tag="LRB02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4660531.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..>6635
                     /gene="radC"
                     /locus_tag="LRB02_002364"
     CDS             6105..>6635
                     /gene="radC"
                     /locus_tag="LRB02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL4660532.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHN"
BASE COUNT         2182 a         1132 c         1455 g         1866 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataat
//
LOCUS       DAFFQY010000103         6613 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFFQY010000103 DAFFQY010000000
VERSION     DAFFQY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.103"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            70..153
                     /locus_tag="LRB02_002365"
     CDS             70..153
                     /locus_tag="LRB02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL4660533.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="LRB02_002366"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="LRB02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL4660534.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="LRB02_002367"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="LRB02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1608..2273
                     /locus_tag="LRB02_002368"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="LRB02_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="LRB02_002369"
     CDS             2309..2554
                     /locus_tag="LRB02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4660535.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="LRB02_002370"
     CDS             2657..3526
                     /locus_tag="LRB02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL4660536.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="LRB02_002371"
     CDS             3507..4241
                     /locus_tag="LRB02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4660537.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5186
                     /gene="ant(6)"
                     /locus_tag="LRB02_002372"
                     /pseudo
     CDS             4274..5186
                     /gene="ant(6)"
                     /locus_tag="LRB02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5183..5725
                     /gene="sat4"
                     /locus_tag="LRB02_002373"
     CDS             5183..5725
                     /gene="sat4"
                     /locus_tag="LRB02_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL4660538.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5818..6612
                     /locus_tag="LRB02_002374"
                     /note="aph(3')-IIIa"
     CDS             5818..6612
                     /locus_tag="LRB02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL4660539.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2318 a          995 c         1387 g         1913 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     4381 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     4441 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     4501 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     4561 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     4621 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     4681 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     4741 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     4801 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     4861 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     4921 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     4981 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     5041 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     5101 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     5161 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     5221 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     5281 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     5341 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     5401 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     5461 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     5521 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     5581 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     5641 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     5701 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     5761 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     5821 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     5881 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     5941 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6001 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6061 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6121 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6181 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     6241 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     6301 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6361 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     6421 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     6481 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     6541 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     6601 gaattgtttt agt
//
LOCUS       DAFFQY010000104         6447 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFFQY010000104 DAFFQY010000000
VERSION     DAFFQY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.104"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(95..550)
                     /locus_tag="LRB02_002375"
     CDS             complement(95..550)
                     /locus_tag="LRB02_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660540.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="LRB02_002376"
     CDS             complement(704..1912)
                     /locus_tag="LRB02_002376"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL4660541.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="LRB02_002377"
     CDS             complement(2024..2356)
                     /locus_tag="LRB02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL4660542.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="LRB02_002378"
     CDS             2504..3379
                     /locus_tag="LRB02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4660543.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="LRB02_002379"
     CDS             complement(3461..4042)
                     /locus_tag="LRB02_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL4660544.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="LRB02_002380"
     CDS             complement(4048..5307)
                     /locus_tag="LRB02_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL4660545.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="LRB02_002381"
     CDS             complement(5309..6355)
                     /locus_tag="LRB02_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660546.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1794 a         1334 c         1100 g         2219 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataa
//
LOCUS       DAFFQY010000105         5925 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFFQY010000105 DAFFQY010000000
VERSION     DAFFQY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.105"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(76..1170)
                     /locus_tag="LRB02_002382"
     CDS             complement(76..1170)
                     /locus_tag="LRB02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL4660547.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="LRB02_002383"
     CDS             1359..2120
                     /locus_tag="LRB02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4660548.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="LRB02_002384"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="LRB02_002384"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL4660549.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="LRB02_002385"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="LRB02_002385"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL4660550.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="LRB02_002386"
     CDS             complement(3760..4773)
                     /locus_tag="LRB02_002386"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL4660551.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="LRB02_002387"
     CDS             4938..5858
                     /locus_tag="LRB02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL4660552.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DAFFQY010000106         5782 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFFQY010000106 DAFFQY010000000
VERSION     DAFFQY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.106"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(192..662)
                     /locus_tag="LRB02_002388"
     CDS             complement(192..662)
                     /locus_tag="LRB02_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL4660553.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="LRB02_002389"
     CDS             992..2632
                     /locus_tag="LRB02_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL4660554.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="LRB02_002390"
     CDS             2786..4108
                     /locus_tag="LRB02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL4660555.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4607)
                     /locus_tag="LRB02_002391"
     CDS             complement(4176..4607)
                     /locus_tag="LRB02_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660556.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4672..5508)
                     /locus_tag="LRB02_002392"
     CDS             complement(4672..5508)
                     /locus_tag="LRB02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL4660557.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1730 a         1151 c         1061 g         1840 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4321 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4381 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4441 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4501 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4561 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4621 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4681 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4741 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4801 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4861 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4921 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4981 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     5041 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     5101 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5161 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5221 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5281 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5341 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5401 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5461 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5521 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5581 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5641 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5701 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5761 aagaggtcgt gagcctgccg tt
//
LOCUS       DAFFQY010000107         5573 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFFQY010000107 DAFFQY010000000
VERSION     DAFFQY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.107"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            129..2189
                     /locus_tag="LRB02_002393"
     CDS             129..2189
                     /locus_tag="LRB02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL4660558.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2272..2841
                     /locus_tag="LRB02_002394"
     CDS             2272..2841
                     /locus_tag="LRB02_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660559.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3016..3822)
                     /locus_tag="LRB02_002395"
     CDS             complement(3016..3822)
                     /locus_tag="LRB02_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660560.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3960..5474
                     /locus_tag="LRB02_002396"
     CDS             3960..5474
                     /locus_tag="LRB02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL4660561.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1986 a          964 c         1127 g         1496 t
ORIGIN      
        1 aacaataaaa aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta
       61 tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact
      121 aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag
      181 ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga
      241 tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca
      301 aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt
      361 tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt
      421 atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa
      481 ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc
      541 agttaaaaca gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa
      601 gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg
      661 atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag
      721 atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga
      781 ctaatacagg aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa
      841 tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc
      901 ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa
      961 gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa
     1021 ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag
     1081 aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt
     1141 ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc
     1201 gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat
     1261 cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat
     1321 tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata
     1381 ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt
     1441 cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga
     1501 tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa
     1561 tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta
     1621 tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg
     1681 ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt
     1741 cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa
     1801 gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc
     1861 aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa
     1921 taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag
     1981 atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc
     2041 tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt
     2101 cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa
     2161 atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag
     2221 tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat
     2281 aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc
     2341 acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg
     2401 gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt
     2461 caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca
     2521 aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg
     2581 tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa
     2641 gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat
     2701 cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact
     2761 tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt
     2821 ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa
     2881 cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat aactaatggc
     2941 atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt
     3001 gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa
     3061 tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat
     3121 aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg
     3181 gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat
     3241 atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg
     3301 gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa
     3361 ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg
     3421 agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc
     3481 cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata
     3541 aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc
     3601 tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa
     3661 aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac
     3721 tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc
     3781 agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt
     3841 atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac
     3901 agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa
     3961 tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg
     4021 gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat
     4081 catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg
     4141 tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg
     4201 ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat
     4261 caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga
     4321 atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc
     4381 ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca
     4441 aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg
     4501 atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg
     4561 aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca
     4621 atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg
     4681 tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt
     4741 tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg
     4801 aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc
     4861 aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc
     4921 aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta
     4981 cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca
     5041 cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat
     5101 tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata
     5161 cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact
     5221 tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg
     5281 gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt
     5341 tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt
     5401 cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt
     5461 cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct
     5521 aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aag
//
LOCUS       DAFFQY010000108         5464 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFFQY010000108 DAFFQY010000000
VERSION     DAFFQY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.108"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            117..1106
                     /locus_tag="LRB02_002397"
     CDS             117..1106
                     /locus_tag="LRB02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4660562.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="LRB02_002398"
     CDS             1088..1549
                     /locus_tag="LRB02_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL4660563.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="LRB02_002399"
     CDS             1599..2063
                     /locus_tag="LRB02_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4660564.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="LRB02_002400"
     CDS             2077..2826
                     /locus_tag="LRB02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4660565.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="LRB02_002401"
     CDS             2826..3656
                     /locus_tag="LRB02_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4660566.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="LRB02_002402"
     CDS             3656..4303
                     /locus_tag="LRB02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4660567.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="LRB02_002403"
     CDS             complement(4401..5177)
                     /locus_tag="LRB02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4660568.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="LRB02_002404"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="LRB02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1874 a          738 c          972 g         1880 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 ttta
//
LOCUS       DAFFQY010000109         4940 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFFQY010000109 DAFFQY010000000
VERSION     DAFFQY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.109"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="LRB02_002405"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="LRB02_002405"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL4660569.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="LRB02_002406"
     CDS             1085..2005
                     /locus_tag="LRB02_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4660570.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="LRB02_002407"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="LRB02_002407"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL4660571.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="LRB02_002408"
     CDS             3311..4789
                     /locus_tag="LRB02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4660572.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1742 a          789 c          944 g         1465 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac
//
LOCUS       DAFFQY010000110         4902 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFFQY010000110 DAFFQY010000000
VERSION     DAFFQY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.110"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            83..1726
                     /locus_tag="LRB02_002409"
     CDS             83..1726
                     /locus_tag="LRB02_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL4660573.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1799..2344)
                     /locus_tag="LRB02_002410"
     CDS             complement(1799..2344)
                     /locus_tag="LRB02_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL4660574.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2367..2852)
                     /locus_tag="LRB02_002411"
     CDS             complement(2367..2852)
                     /locus_tag="LRB02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4660575.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2842..3654)
                     /locus_tag="LRB02_002412"
     CDS             complement(2842..3654)
                     /locus_tag="LRB02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL4660576.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3793..>4902)
                     /locus_tag="LRB02_002413"
     CDS             complement(3793..>4902)
                     /locus_tag="LRB02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL4660577.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1577 a          968 c          938 g         1419 t
ORIGIN      
        1 cgtttctagt taccggtaac aaggatactt ctaaaataga atcggttata atcaagaggg
       61 accgttaaag gagtgaaacg atatgataca agaacaaaaa tggtggaaag aagcagtagg
      121 ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc gatggtcaag gagacatcaa
      181 tgggatccgt gaaaaactta attatttgaa agatcttggt atcggcttta tctggatcaa
      241 tccgatctat caatctccat ttgtcgataa tggttatgac gtaagtgact atcaagatat
      301 agaagagaga ttcggaacga tggaagattt tgatttattg ctaaaagaag cacatgaatt
      361 agggatcaaa gtgattatgg atcttgtcat caaccatagt tcggatcagc atcaatggtt
      421 tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat tattacattt gggtcgacgg
      481 ggtagatggc aaagaaccga ataattggac atcgattttc ggcggttctg cttgggagta
      541 ttctcatgaa actggacagt attatctgca tgtttttgca aaagaacaac cagacttaaa
      601 ttgggaaagc gagaaattga aagaagaact attcaatatg atccgatggt ggttggataa
      661 agggatagat ggatttcgtt tagatgcgat ttctcatgta aaaaaagatg aatattcagt
      721 aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt
      781 gaccgatttg aaacatgttt ttgaagagta cgatatcatg actgtcggag aagcaagtgg
      841 tgtcactgca gaagaaggac cacaatgggt agggaaagac ggttatttcg atatgatatt
      901 cgaatttgat catatccata tctggcagca agaaaaagaa gggcaattag atgtccttaa
      961 attgaaactt gcattaagcg cttggcaaac ttcattggat ggtatcggat ggaacgcttt
     1021 atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga
     1081 tttttgggct atgtctgcaa aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc
     1141 atttatttat caaggacagg aaatcggaat gacgaatatg ccctttgaat cgatcgatca
     1201 agttgatgca gtagattcga aaagacttta taaaaggctg cttgcggaag gtaagacaag
     1261 agaagaagca ttggatatca ttcgtgaaac aacgagagac aacagcagga cacccatgca
     1321 atggacttcg gaacaatatg ctggtttttc tacacatgaa ccttggctga tcactaatcc
     1381 aaataccaaa acgatcaatg tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt
     1441 ctataagaac atgatccgca ttagacaaac caataaaggt ctgatatacg gttcttataa
     1501 agaatatctt catgaacatc cacaacttta tgtctatgaa agatatttag aagatgaaca
     1561 atatcttatt atggtaaact taacagaatc acttgctgac tatgaacttc caaaagaagc
     1621 agatcagtcc tggacattac tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg
     1681 catactagca ccttatgaag ctcggttata taaaacaaac aagtaaaact tcctcgttaa
     1741 agttcattag ttggactttg cgaggaagtt tttcagattg ctctgtcaat tactcatttc
     1801 aataattcgc tctaggacca ccgatatatt taggacgaga gcgaagggct gtcacgtgag
     1861 cttgtccaag ggttttcaaa cgaggtctga caggtacttg gacatgtttg atatgcatac
     1921 cgattgatgt gtcaccgata tcgataccgg cttgtccaac tacgtgttcc acttcgacag
     1981 gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc tccagcatgt agtgcaggaa
     2041 caacagagac aatttcgaaa ttttttgctt ctgccactgt tcgttctact actaatgctc
     2101 ggttcaaatg ttcacaacct tggacagcta gcgcaatttc tttaggatcc aacaactctt
     2161 ttaacgtacg gatgatccat tgaccaactt ccgcactgga attttttcca atatgtcctc
     2221 caacgacttc gcttgtgctg catcctaaaa caaaaagatc accttttttc aagtgtgcct
     2281 cagccaatat gtcattaact atttcagtca attggtcttt taattctttt tctgacaact
     2341 tcattttcta aaaccttctt tttcaattaa tattgcttcc gtgcttgaat acgttgaaga
     2401 cttgcagtta taggaaaggc aagtcccatt ccaatcagat tttggaagat gtttcctggt
     2461 atagaagcca ctccagccgg ccaatcatat aaaatccaac cggcgatacc atagccgatg
     2521 atcataacta agcttcctaa taaaagccct aatccccaca tacgagtaga agatttgctg
     2581 aaagaaccag caacatatcc ttgtaacccg tggatcacta atgaaaaaac tgcccactgc
     2641 ggatatcctg aaatcaaatc gatcaataga ccactggaag caccaacaac taatccaccc
     2701 gttggaccat aaagaaaagc agcggtatag atcccggcct cgcataatgt cacaaatcca
     2761 ttcgtagccg gtacagggat caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc
     2821 atagcataga tcgttaattg cttagttttc atttctcatt cctccttttt gaataccgcc
     2881 agaccagaca cttccggtat gatcgttcat cagcacgcca ttagaaatac aatgggcaac
     2941 aaatttttta ctttctgcta ctgctttggt aaaatctgcc cctaacacaa gattcgctgt
     3001 tattgcagaa gaaaaggcac aaccggcccc gtttactgtt gtagtgtcga tttttttatg
     3061 agaaaatacc tcataatgat caattgaata gagaagatcc acagcttcat ctccagccat
     3121 ccttgtgccg cctttgatca caatgttttt tgttcctgaa gcagtgagtt ctttagcgag
     3181 atggatcatt tctgttaacg aagagggaag agcttgacca gaaagtaaag cagcttctgc
     3241 caaattaggc gtaatcatcg taacaagtgg aaaaagttct tcgatgagct gcgaaatata
     3301 caattgattg atcacagctt cagtttcttt gaatgccaaa acagggtcta aaatgattgg
     3361 gatattttgt tttgttttta tgaagtctcg aacaaccatg attgcttcac ttgaattcaa
     3421 taacccaatt ttgataccat ctaatgaagt cattttatca atcgaaacta actgtcgttt
     3481 aagcaattcc aaagatatag attccagcat aaatcgctca ttaatatcta gtgcccctat
     3541 agaagtcaag gcgcttagtc cgaatagacc aaaattctca aaagttttta aatctgtttg
     3601 gattcctcca ccagcaaatg gatcagaacc gccaattgtc aagattcttt tcataacatt
     3661 ttcctccatg aataagagta taacgaaatt ttcaaaaaag aaaaacagcc aattggcata
     3721 tttttatctc atccaattta aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct
     3781 ttccttaaat agttaggact tttgtgattt taattctttc tcgatttgaa gccgttgcac
     3841 tttcattgta gctgttcgcg ggattgcatc aaaatcacgg atgataggga cgttcaagcg
     3901 aggaagttca tggacttgct cccaccatcg attccaatcc atttctttat caggagctaa
     3961 agcaataatc ggttgtggag aatgattttt atcccttaca atcactactt ctgccaaaaa
     4021 atctaacgaa tctaataaaa agtcttcaat agctaatgta ctattgatcg tttcgatcaa
     4081 atccacttgc cgatctttca aaaataggtg gccatgctta tccataaaac cgtaatctcc
     4141 gctatcccac caatcgccat aaacattttc ctggaaccgt gcatcttcct tgtaataagt
     4201 caaagcccgt cctttagaca gtaattgtat atgaccatct gtgttttcag gtaggacatt
     4261 cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc agtccaacgc ccatatctct
     4321 tgcatcactt gttttcaaac tctctagtgt atgagcctta agaatcattg gtccgcattc
     4381 actttggcca tatacttgaa gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt
     4441 cttcattgtc tggttgttta tagcatcaaa agtggagtga tagtatcgga tacccgaaaa
     4501 agcttctgga tgctccttag cagtaaaacg ccactgaaca aagttattcg gatgggtctc
     4561 tagtgcaatt ggccggtgag cttccaacat atgaacaact tttgaacttt gtgcgtttgc
     4621 taaaggcatc ataggaaatc ccatggacat caaagaagag ataccaatat tgaatctgga
     4681 atgcactgga gagatgtgaa aagcgattaa tttcttttcc gaaatttttg tgaaaactgt
     4741 tttttgccat ttcgtccgcc atcccataga atgagcagaa tgacaaataa gtttagggat
     4801 accagttgta ccagaagtat gggtcatata agaaatttca tttttgtcta aaaatgattg
     4861 agaaacagac aaagcaggtt gttccattaa ataaagaata gg
//
LOCUS       DAFFQY010000111         4771 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFFQY010000111 DAFFQY010000000
VERSION     DAFFQY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.111"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            221..655
                     /locus_tag="LRB02_002414"
     CDS             221..655
                     /locus_tag="LRB02_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4660578.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="LRB02_002415"
     CDS             678..998
                     /locus_tag="LRB02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660579.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="LRB02_002416"
     CDS             1139..1330
                     /locus_tag="LRB02_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660580.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="LRB02_002417"
     CDS             complement(1401..1658)
                     /locus_tag="LRB02_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL4660581.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="LRB02_002418"
     CDS             1816..2079
                     /locus_tag="LRB02_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660582.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="LRB02_002419"
     CDS             2103..2291
                     /locus_tag="LRB02_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660583.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2374..2559)
                     /locus_tag="LRB02_002420"
     CDS             complement(2374..2559)
                     /locus_tag="LRB02_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660584.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            2774..3145
                     /locus_tag="LRB02_002421"
     CDS             2774..3145
                     /locus_tag="LRB02_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4660585.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3295..3367)
                     /locus_tag="LRB02_002422"
     tRNA            complement(3295..3367)
                     /locus_tag="LRB02_002422"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3332..3334),aa:Gln,seq:ctg)
     gene            complement(3413..3841)
                     /locus_tag="LRB02_002423"
     CDS             complement(3413..3841)
                     /locus_tag="LRB02_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660586.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(3901..4659)
                     /locus_tag="LRB02_002424"
     CDS             complement(3901..4659)
                     /locus_tag="LRB02_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL4660587.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1668 a          736 c          931 g         1436 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca gcagcattgt
     2341 cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt taaatttgtt
     2401 tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt tataaggtcc
     2461 taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat
     2521 catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat cctttgctat
     2581 caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac gtcaataata
     2641 tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa
     2701 aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga atatataaca
     2761 aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga ttagtggtat
     2821 tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc ctggtatact
     2881 tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga
     2941 ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa
     3001 agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat
     3061 tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa aagttccgga
     3121 gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc ccgattttat
     3181 tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca ataatccccc
     3241 tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat caaattggga
     3301 tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc agtttggcta
     3361 catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca agttattttt
     3421 cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc ttttctaatc
     3481 cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac tgatattctt
     3541 tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag
     3601 tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg tactcttgat
     3661 ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg agggtagcag
     3721 gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt cccttgcagt
     3781 cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg aatttttcca
     3841 ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt gtttgaatta
     3901 ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc
     3961 gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa tattttcttt
     4021 gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt ctccattatt
     4081 tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag tcacagaagt
     4141 ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag catcggtttt
     4201 tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa ctgggggaac
     4261 atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt cattaggatc
     4321 gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag ctaactggtt
     4381 tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga cttcacagcc
     4441 attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta aaacttctgc
     4501 tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg
     4561 ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag cagctgtgcc
     4621 ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc cttttcagct
     4681 gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat attttaatat
     4741 gtaggaaata attagttttt aaggaatatg t
//
LOCUS       DAFFQY010000112         4726 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFFQY010000112 DAFFQY010000000
VERSION     DAFFQY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.112"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="LRB02_002425"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="LRB02_002425"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL4660588.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="LRB02_002426"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="LRB02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL4660589.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="LRB02_002427"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="LRB02_002427"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL4660590.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DAFFQY010000113         4571 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFFQY010000113 DAFFQY010000000
VERSION     DAFFQY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.113"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(338..1291)
                     /locus_tag="LRB02_002428"
     CDS             complement(338..1291)
                     /locus_tag="LRB02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4660591.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="LRB02_002429"
     CDS             complement(1314..2069)
                     /locus_tag="LRB02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4660592.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="LRB02_002430"
     CDS             complement(2066..3025)
                     /locus_tag="LRB02_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL4660593.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="LRB02_002431"
     CDS             complement(3018..3968)
                     /locus_tag="LRB02_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL4660594.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="LRB02_002432"
     CDS             4118..4570
                     /locus_tag="LRB02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL4660595.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1490 a          962 c          775 g         1344 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa a
//
LOCUS       DAFFQY010000114         4563 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFFQY010000114 DAFFQY010000000
VERSION     DAFFQY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.114"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(105..629)
                     /locus_tag="LRB02_002433"
     CDS             complement(105..629)
                     /locus_tag="LRB02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660596.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LRB02_002434"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LRB02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL4660597.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LRB02_002435"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LRB02_002435"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL4660598.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LRB02_002436"
     CDS             complement(2351..3031)
                     /locus_tag="LRB02_002436"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL4660599.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LRB02_002437"
     CDS             3157..4026
                     /locus_tag="LRB02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4660600.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..>4563
                     /locus_tag="LRB02_002438"
     CDS             4052..>4563
                     /locus_tag="LRB02_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660601.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNL"
BASE COUNT         1291 a          977 c          842 g         1453 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aag
//
LOCUS       DAFFQY010000115         4504 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFFQY010000115 DAFFQY010000000
VERSION     DAFFQY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.115"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..1730)
                     /locus_tag="LRB02_002439"
     CDS             complement(<1..1730)
                     /locus_tag="LRB02_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HBL4660602.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLP"
     gene            1876..2451
                     /locus_tag="LRB02_002440"
     CDS             1876..2451
                     /locus_tag="LRB02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4660603.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            2665..3360
                     /gene="vanR"
                     /locus_tag="LRB02_002441"
     CDS             2665..3360
                     /gene="vanR"
                     /locus_tag="LRB02_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL4660604.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3338..4492
                     /locus_tag="LRB02_002442"
     CDS             3338..4492
                     /locus_tag="LRB02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL4660605.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1436 a          846 c          871 g         1351 t
ORIGIN      
        1 ggtagcatct gataaaggct tgcactaaat ttttttgctt cttctggaac atctttttct
       61 aagcgtgcaa gtgatagctt tcctttttca agagaaaccc catctaactt attggaattg
      121 gcagctaacc actttaacct ttcattaaag ctgctggttc tctccgttat ataatcttcg
      181 aatgataaac taactgataa tctcgtattc cccttcgatt gattccatgt atcttccgaa
      241 aacaaatatt cctcaaaatc cctatattgt ctgctgccaa caatggaaac atctcctgcc
      301 cgaacatgct cccgaagttc tgttaaaaca gccatttcat agtaatgacg attaattgtt
      361 gtaccatcat cctcgtataa atgtcttttc catcgttttg aaataaaatc cacaggtgag
      421 tcatcaggca cttttcgctt tccagattcg ttcattcctc ggataatctc aacagcttgt
      481 aaaagtggct catttgcctt tgtagaatga aattccaata ctcttaatag cgttggcgta
      541 tattttctta gtgaataaaa ccgtttttgc agtaagtcta aataatcata gtcggcagga
      601 cgtgcaagtt cctgagcctc ttctactgaa gagacaaagg tattccattc aataaccgat
      661 tctaaaacct taaaaacgtc taatttttcc tctcttgctt taattaatgc ttgtccgatg
      721 ttcgtaaagt gtataacttt ctcatttagc tttttaccgt tttgtttctg gatttcctct
      781 tgagccttac gaccttttga taacaaacta agtatttgcc tatcatgaat ttcaaacgct
      841 ttatccgtta gctcctgagt aagttgtaat aaatagatgg ttaatatcga ataacgttta
      901 ttttcttgaa agtcacggaa tgcatacggc tcgtatcttg agcctaagcg agacagctgc
      961 aacaggcggt tacggtgcaa atgactaatt tgcactgttt ctaaatccat tcctcgtatg
     1021 tattcgagtc gttctattat ttttagaaaa gtttcgggtg aaggatgacc cggtggctct
     1081 tttaaccaac ccaatatcgt tttattggat tcggatggat gctgcgaggt aataatccct
     1141 tcaagctttt ctttttgctc atttgttaga gatttactaa ccgtattaaa tagcttcttt
     1201 tcagccattg cccttgcttc ccacaccatt ctttcaagtg tagtgatagc aggcagtata
     1261 attttgtttt ttcttagaaa atctatgcat tcatgcagta gatgaatggc atcaccattt
     1321 tccaaagcta attgatgaag gtacttaaat gtcattcgat attcactcag ggtaaaagtt
     1381 acaaagtcgt attcacttcg aatttctttc aaatgatccc aaagtgtatt ttccctttga
     1441 ggataatgat caagcgagga tggactaaca ccaatctgtt tcgatatata ttgtatgacc
     1501 gaatctggga tgcttttgat atgagtgtat ggccaaccgg gataccgaag aacagctaat
     1561 tgaacagcaa atcctaaacg gttttcttcc ctccttcgct tattaactat ttctaaatcc
     1621 cgtttggaaa aagtgaagta ggtccccagt atccattcat cttcagggat ttgcataaaa
     1681 gcctgtctct gttccggtgt aagcaattct ctacctctcg caattttcat tcagtatcat
     1741 tccatttctg tattttcaat ttattagttc aattatatat caatagagtg tactctattg
     1801 atacaaatgt agtagactga taaaatcata gttaagagcg tctcataaga cttgtctcaa
     1861 aaatgaggtg atattttgcg gaaaatcggt tatattcgtg tcagttcgac taaccagaat
     1921 ccttcaagac aatttcagca gttgaacgag atcggaatgg atattatata tgaagagaaa
     1981 gtttcaggag caacaaagga tcgcgagcaa cttcaaaaag tgttagacga tttacaggaa
     2041 gatgacatca tttatgttac agacttaact cgaatcactc gtagtacaca agatctattt
     2101 gaattaatcg ataacatacg agataaaaag gcaagtttaa aatcactaaa agatacatgg
     2161 cttgatttat cagaagataa tccatacagc caattcttaa ttactgtaat ggctggtgtt
     2221 aaccaattag agcgagatct tattcggatg agacaacgtg aagggattga attggctaag
     2281 aaagaaggaa agtttaaagg tcgattaaag aagtatcata aaaatcacgc aggaatgaat
     2341 tatgcggtaa agctatataa agaaggaaat atgactgtaa atcaaatttg tgaaattact
     2401 aatgtatcta gggcttcatt atacaggaaa ttatcagaag tgaataatta gccattctgt
     2461 attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa ctataaaata ttaacagcct
     2521 cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca tcatcttaag aaattcttag
     2581 tcatttatta tgtaaatgct tataaattcg gccctataat ctgataaatt attaagggca
     2641 aacttatgtg aaagggtgat aactatgagc gataaaatac ttattgtgga tgatgaacat
     2701 gaaattgccg atttggttga attatactta aaaaacgaga attatacggt tttcaaatac
     2761 tataccgcca aagaagcatt ggaatgtata gacaagtctg agattgacct tgccatattg
     2821 gacatcatgc ttcccggcac aagcggcctt actatctgtc aaaaaataag ggacaagcac
     2881 acctatccga ttatcatgct gaccgggaaa gatacagagg tagataaaat tacagggtta
     2941 acaatcggcg cggatgatta tataacgaag ccctttcgcc cactggagtt aattgctcgg
     3001 gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag taaaggagca gaacgaaaat
     3061 gttatcgtcc actccggcct tgtcattaat gttaacaccc atgagtgtta tctgaacgag
     3121 aagcagttat cccttactcc caccgagttt tcaatactgc gaatcctctg tgaaaacaag
     3181 gggaatgtgg ttagctccga gctgctattt catgagatat ggggcgacga atatttcagc
     3241 aagagcaaca acaccatcac cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc
     3301 attgataatc cgaaatatat aaaaacggta tggggggttg gttataaaat tgaaaaataa
     3361 aaaaaacgac tattccaaac tagaacgaaa actttacatg tatatcgttg caattgttgt
     3421 ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc cgagggaaac ttggggattg
     3481 gatcttaagt attttggaaa acaaatatga cttaaatcac ctggacgcga tgaaattata
     3541 tcaatattcc atacggaaca atatagatat ctttatttat gtggcgattg tcattagtat
     3601 tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa tactttgacg agataaatac
     3661 cggcattgat gtacttattc agaacgaaga taaacaaatt gagctttctg cggaaatgga
     3721 tgttatggaa caaaagctca acacattaaa acggactctg gaaaagcgag agcaggatgc
     3781 aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac ttggcgcacg atattaaaac
     3841 gccccttaca tccattatcg gttatttgag cctgcttgac gaggctccag acatgccggt
     3901 agatcaaaag gcaaagtatg tgcatatcac gttggacaaa gcgtatcgac tcgaacagct
     3961 aatcgacgag ttttttgaga ttacacggta taacctacaa acgataacgc taacaaaaac
     4021 gcacatagac ctatactata tgctggtgca gatgaccgat gaattttatc ctcagctttc
     4081 cgcacatgga aaacaggcgg ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc
     4141 tgataaactc gcgagagtct ttaacaacat tttgaaaaac gccgctgcat acagtgagga
     4201 taacagcatc attgacatta ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa
     4261 gaacactgga agcatcccaa aagataagct agctgccata tttgaaaagt tctataggct
     4321 ggacaatgct cgttcttccg atacgggtgg cgcgggactt ggattggcga ttgcaaaaga
     4381 aattattgtt cagcatggag ggcagattta cgcggaaagc aatgataact atacgacgtt
     4441 tagggtagag cttccagcga tgccagactt ggttgataaa aggaggtcct aagagatgta
     4501 tata
//
LOCUS       DAFFQY010000116         4454 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFFQY010000116 DAFFQY010000000
VERSION     DAFFQY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.116"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(212..529)
                     /locus_tag="LRB02_002443"
     CDS             complement(212..529)
                     /locus_tag="LRB02_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL4660606.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(530..784)
                     /locus_tag="LRB02_002444"
     CDS             complement(530..784)
                     /locus_tag="LRB02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4660607.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1143..1547
                     /gene="tnpA"
                     /locus_tag="LRB02_002445"
     CDS             1143..1547
                     /gene="tnpA"
                     /locus_tag="LRB02_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL4660608.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1564..2712
                     /locus_tag="LRB02_002446"
     CDS             1564..2712
                     /locus_tag="LRB02_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660609.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(2935..3153)
                     /locus_tag="LRB02_002447"
                     /pseudo
     CDS             complement(2935..3153)
                     /locus_tag="LRB02_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3419..3613)
                     /locus_tag="LRB02_002448"
     CDS             complement(3419..3613)
                     /locus_tag="LRB02_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660610.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(3713..3799)
                     /locus_tag="LRB02_002449"
                     /pseudo
     CDS             complement(3713..3799)
                     /locus_tag="LRB02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         1525 a          749 c          704 g         1476 t
ORIGIN      
        1 ttattcacca agcgatcccc gtagttccta cggttcttaa tttttctcta gactaatgat
       61 tgtatcttta gaccttcggc cataacatgg agcaaaataa aaaggtacta agtcttatat
      121 atcaatcttc gagtaaccga cggttaacaa ccatatcgag cggcattaat cacccacctg
      181 tagaggatga gcgacttttt cctaattatt gttaaaagcc tagtttcatt tgtttagatt
      241 ggaaaatacg tcctacaagt atctcttttt tttcatggtc gatccgataa aataaggcat
      301 agcttttgcc gatcacgatc cgatatgttg ggatatccat tccatagatt tcggaaactt
      361 cctcatgcat agttggaaaa tttttggtta gttctaaagc cttctggatc gcacctacaa
      421 atctttttat gctggctgga gaaagagcaa gctcattttc ccattcttct attatcgttt
      481 caaggctggt ctgaaaactt ttagaataaa ctagcgtgta ctctttcatc tattcactcc
      541 agccgtattt tccgaaagct tttaccattt caatttctga aactgttaaa cctttctcaa
      601 agtcttggaa actttcttca atcaatttgc gatcgtgttc ttttgctatt tgagtagtat
      661 ctaatatgaa taagttgtct tcttgtttgg cttggataat gtctccgctt tttaatccaa
      721 gagagcttgg aaggatcact cccacgctat tgccgatctt tctaaccttt agttcttttc
      781 ccatgttatc acactccttt tagtttttga tgaataaatt ataacatgtt ataacaacaa
      841 ttgccaacgg tgtttcttaa gggattccac tccatattat tattggacaa aataagccac
      901 ttagaacgct gaaaacatat acaaacgata tcaaaatacg agtgaactac tcaccataca
      961 cttccgttaa catccattta acagaattag gcagaagtcg cccatcctct ataggtgggt
     1021 gatgaatgcc gttcggtatg agggttaccg ttggtaactc gaagaccgac aactaaggcg
     1081 tattgccttt ttattttgtc ccatgttaca atcagtctat acaagaaatg agttgatata
     1141 acatggaatt agatacaaat aatcattcag tatttcttct ttattaccac cttgttttag
     1201 taacgaaata tcgtagacaa gtgattgatg atgaaatatc tgattatgcc aaaactactt
     1261 ttgaaagaat ttcagaatcg tatcacatta ctttagtgga gtggaatcat gataaagacc
     1321 acgttcatat tatgttcaaa gctcaaccta aaacagaatt gacaaaattc atcaatgcct
     1381 ataaaagtgc cagctcaaga ttgataaaac gtgactttcc aagagtcaaa caatttcttt
     1441 ggaaagaaat gttctggtct aaaagctttt gtcttctgac aactggtggc gcaccaattg
     1501 atgtgattaa gaagtatatc caaaatcaag gaaacaatca taaatagaaa gtaggtgaac
     1561 tttatggaac aactaaaagc atacaaattc agaatctatc caacagagga acaagaaatc
     1621 ttctttgcca aatcttttgg ttgtgtccgt aaggtctaca atctaatgct tgatgaccga
     1681 aaaaaggcgt atgaagaagt taaaaatgat tcttctaaaa aaatgacttt tccgacacca
     1741 gctaaataca agaaagaatt tccgtttttg aaagaaattg atagccttgc tttagccaat
     1801 gcccaactta atttagataa agcatacaag aatttttttc gggataaatc cgttggattt
     1861 ccacgtttca aaagtaagaa aaatcctgtt cagagttata caacgaataa tcaaaatggt
     1921 acaattgctt tgattgatag taaattcatc aaagttccta aattgaagtc attagtaaga
     1981 attaagcttc atagacaacc aaaagggatt atcaaatctg ctacaatatc acgtcattct
     2041 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
     2101 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
     2161 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
     2221 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
     2281 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
     2341 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
     2401 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
     2461 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
     2521 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
     2581 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
     2641 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
     2701 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
     2761 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
     2821 ccacttcaag cgttaagaac cgtcaggttt agctaagtgg tgagtagttc acgtaggtat
     2881 ttttattata tatagattta acatatgtcg gtcggttgat tgatatagct aaagttataa
     2941 ctcaaccgaa gccgattcat ctacacgaat aaagttgcga gtgttctcgg ctaattcata
     3001 aaaattgtta tgtgttccat atagcaatac tggaataact ttattttctt gaatctcata
     3061 agcaactcta tacacggtga ccgcatactt aaatccatta gaccaattac ctgataaacc
     3121 tccagttttt tgctcttact taaatggatc aatggcaatt tcatcacaaa cctatagagg
     3181 atgagtgact tctgcctaat tacgttaaaa aatattgaaa gtaactctat gtagcaaaaa
     3241 atatctagaa tttcatttcc tcaaaactga tcgatattgt atctaaaaat atatactacc
     3301 taaaataggt agtatatatt aaaatatgtg cttttgttag aaaaatcaat acataaatta
     3361 tcatatctgt atatgtcatt taggtatttc agttatgtta taatagtttt gctgtctgct
     3421 accccggcct aatctggcaa ggggaggagg agatataatg aaataccttt ttgattttgt
     3481 ttttgcaatc aagccacttg cagatgtagt gtgcagttac ctctgcaaat ggcttgattg
     3541 caaaattgcc aaaaaagaca gtaagcctac ccgttaattc tataatatag aaacgtaaaa
     3601 aaaaccccat cagcctgcac gctgatgggg tttggtgata taatgaaata cctctttgtt
     3661 actcataatt gtaccataat tagtcacgaa tgcaaccaat agaaaaaact acttagaacg
     3721 gtagatcatc gctaataaca atgcattgtc caccaaatgg attttgtgaa gcattaaata
     3781 cattgttatt aattgtcgct gcttttaact aggcctctcc ccttctatta taaaagaaga
     3841 tatatatcat ttcttaatta cctactacct taaaccaaaa tttaagtact acctaattta
     3901 agtattactt aaacaatcta aaaaatagtt atcccaattg ttatgctttt ataatacgat
     3961 tatttgtaac aaaatgaata ttatataatt aaacactacc atatttaggt agtgtttaat
     4021 tattagcaaa acaaaaaata tttattctat tctcttaact tcaactaagt tatgctgcac
     4081 acctttatat aaatcatatg caactcatta gctagacaaa ctacaagcat gtagttttat
     4141 ctgttaatta tgtactacct aaataaagta gtttataatt agcaagtata tatatttaaa
     4201 aatttaagat taattttttt atgacttgta caaatttatc tgatattctc ttattaagaa
     4261 ctacctaatt tgggtagttc ttatttttta cattttaagt agtatttaat tgcaatttgg
     4321 tagtattttt ttattatctt attcttagaa cggtagtact taatataaat ataggtagta
     4381 tctaatttaa gtattaatgg tagtatctaa attaacaggt agtatgtaac tactttaaag
     4441 taattttttt caga
//
LOCUS       DAFFQY010000117         4430 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFFQY010000117 DAFFQY010000000
VERSION     DAFFQY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.117"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(543..2048)
                     /locus_tag="LRB02_002450"
     CDS             complement(543..2048)
                     /locus_tag="LRB02_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4660611.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LRB02_002451"
     CDS             complement(2045..2731)
                     /locus_tag="LRB02_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4660612.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LRB02_002452"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LRB02_002452"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL4660613.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DAFFQY010000118         4362 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFFQY010000118 DAFFQY010000000
VERSION     DAFFQY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.118"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            149..1072
                     /locus_tag="LRB02_002453"
     CDS             149..1072
                     /locus_tag="LRB02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL4660614.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDLITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1078..2331
                     /locus_tag="LRB02_002454"
     CDS             1078..2331
                     /locus_tag="LRB02_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL4660615.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIYYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="LRB02_002455"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="LRB02_002455"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL4660616.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1502 a          688 c          939 g         1233 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atttgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatctat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tt
//
LOCUS       DAFFQY010000119         4218 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFFQY010000119 DAFFQY010000000
VERSION     DAFFQY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.119"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            10..210
                     /locus_tag="LRB02_002456"
     CDS             10..210
                     /locus_tag="LRB02_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL4660617.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="LRB02_002457"
     CDS             288..962
                     /locus_tag="LRB02_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL4660618.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            955..1119
                     /locus_tag="LRB02_002458"
     CDS             955..1119
                     /locus_tag="LRB02_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660619.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            1195..2337
                     /gene="dcm"
                     /locus_tag="LRB02_002459"
                     /pseudo
     CDS             1195..2337
                     /gene="dcm"
                     /locus_tag="LRB02_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2327..2617
                     /locus_tag="LRB02_002460"
     CDS             2327..2617
                     /locus_tag="LRB02_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660620.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="LRB02_002461"
     CDS             2876..4060
                     /locus_tag="LRB02_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL4660621.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4057..4194
                     /locus_tag="LRB02_002462"
     CDS             4057..4194
                     /locus_tag="LRB02_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL4660622.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DAFFQY010000120         4149 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFFQY010000120 DAFFQY010000000
VERSION     DAFFQY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.120"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            558..3197
                     /locus_tag="LRB02_002463"
     CDS             558..3197
                     /locus_tag="LRB02_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4660623.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="LRB02_002464"
     CDS             complement(3365..4063)
                     /locus_tag="LRB02_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL4660624.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1260 a          790 c          904 g         1195 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttat ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttatccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgattgtca
     1201 ctgccattgg tatggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaagcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc atttgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggta actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactctca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacaggg acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagatgctg
     2521 ttagtcaagg gcgttcagtt tatcagaata ttcgtaaagc aatcaacttt ttactaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagca accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctaccaaccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgcg gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtagataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagccgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcatctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaataaga taataggaat cataataaga agaatagcag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctctt
     4141 ttttgttat
//
LOCUS       DAFFQY010000121         4035 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFFQY010000121 DAFFQY010000000
VERSION     DAFFQY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.121"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            178..774
                     /locus_tag="LRB02_002465"
     CDS             178..774
                     /locus_tag="LRB02_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4660625.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="LRB02_002466"
     CDS             898..2697
                     /locus_tag="LRB02_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL4660626.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="LRB02_002467"
     CDS             complement(2975..3187)
                     /locus_tag="LRB02_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660627.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="LRB02_002468"
     CDS             3649..3798
                     /locus_tag="LRB02_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660628.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1185 a          688 c          712 g         1450 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat attta
//
LOCUS       DAFFQY010000122         3923 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFFQY010000122 DAFFQY010000000
VERSION     DAFFQY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.122"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(573..1130)
                     /locus_tag="LRB02_002469"
     CDS             complement(573..1130)
                     /locus_tag="LRB02_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660629.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="LRB02_002470"
     CDS             complement(1309..1920)
                     /locus_tag="LRB02_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660630.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="LRB02_002471"
     CDS             complement(2033..2290)
                     /locus_tag="LRB02_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660631.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="LRB02_002472"
     CDS             complement(2887..3165)
                     /locus_tag="LRB02_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660632.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="LRB02_002473"
     CDS             complement(3199..3450)
                     /locus_tag="LRB02_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660633.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAFFQY010000123         3883 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFFQY010000123 DAFFQY010000000
VERSION     DAFFQY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.123"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            91..3123
                     /locus_tag="LRB02_002474"
     CDS             91..3123
                     /locus_tag="LRB02_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL4660634.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="LRB02_002475"
     CDS             3313..>3883
                     /locus_tag="LRB02_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBL4660635.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DAFFQY010000124         3809 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFFQY010000124 DAFFQY010000000
VERSION     DAFFQY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.125"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            93..2192
                     /locus_tag="LRB02_002476"
     CDS             93..2192
                     /locus_tag="LRB02_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL4660636.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2388..3251
                     /locus_tag="LRB02_002477"
     CDS             2388..3251
                     /locus_tag="LRB02_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL4660637.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3356..>3809
                     /locus_tag="LRB02_002478"
     CDS             3356..>3809
                     /locus_tag="LRB02_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4660638.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1523 a          636 c          772 g          878 t
ORIGIN      
        1 gtaaatccta aaaataagct attggcgtga cgtatagaat atgatagatt aaatggtagc
       61 gtaaacctag taaaggagcc gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga
      121 aaaaaatgga gcagaatact atctgtccag actgcggtca tcaaataact gcagaagatc
      181 agcaaataaa agaatcaatt gaagaaacaa atacagatgc agctatgcag gataaatcaa
      241 ctgaagatgc agtctttcct gatacagaat taaacgatcc aatcgaatgg tcggaattga
      301 aagatttacc tctagaatct gtaatggaat tattcgaaaa agcagaacca gaagacgaag
      361 aaccacctgc tcacgatcaa gtaactaata tagagaaaaa tgaacagaag aaagcaactg
      421 ttcatgagac ttcggaagaa tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa
      481 aaagaagagt agctgaactg aaagaaatcg tcgataatga ggaagaaaat tcgatactat
      541 ccgcttatat caaagcacat cgtgaagata caaaagaaga gcacgcgaaa gagctgctca
      601 aaatgatcag tgaaaaaatt gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa
      661 ctgaaaaaga ctctgataga gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg
      721 taaaaacgga tatcacagaa aaaactgaag tatttactga aaaacaatta gaaaaggaaa
      781 aaacgaataa actgccggaa gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt
      841 caacagagcc cgatgaaata aatagtgaat ccgatgctga aaatcaatct gtaagcaaag
      901 agggtactga tcttgaagca aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag
      961 cagaagcgaa atcaataaca atcgtgccag atactgaaaa aacacatcaa gaatcaaact
     1021 tagaaaaaac agatccagat aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg
     1081 ttttagcagc agtacttcta ctcggtggag gaagctgggc ttattatgac catcatcaaa
     1141 aagtagaagc cgaagtagca gcagaagcaa aaagacagca acaaatggaa gaacttcatg
     1201 agaaacttgc tgccttttat acagatgatc accatcaatt tattcgtgca gatatgattc
     1261 atcaagattt atcaaaacta aaaaatgatt tatccagtct taagaatgaa aaaggatatt
     1321 cagagttaga aacaacttac caagatatcc aatctaagat ccgagatatc caaaaagtga
     1381 atgagtggtt catagcacct gccatcgtcg atgatcatct agcagatgac atcaagttga
     1441 aagcagataa agagatccaa gcaatcgaaa cggataacac agattttgga aaattgattc
     1501 aacaagcaca aacagaagct aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa
     1561 cagctatagt ctttaaagac ggaaaagtag tggattcagc aacacgcgac caatacaaga
     1621 cggcaaaaga agcagtagaa aaagtgaaaa atacagatct atctgctcag ttgaatgaac
     1681 aattgaaaca agtagataag atgctcactg ataaagagaa aaaagaagaa gaggaaaaga
     1741 aaaaagcaga agaagcgaaa aaagcagctg aagaacaagc aaaacaagcc gctcaagcag
     1801 cagctgcagc tcaagccgct caacaagctg cggtcgcaca agcagcacag caagaagcgg
     1861 catcctcgac cccttcctct tcaaactcag cgaatcagcc aatcaaatct gccagagcaa
     1921 gtgatattgc cgatgcatct aatccagcat ggacgtgggc accaggtgtc caaagcaaag
     1981 tgattgctac atgtatccaa cgtggatata tcgttgaagg aggttacaga ttagaaaaag
     2041 ccagaatcga aaatggggaa ggctattata atctatatgc cacttcaaca aaatctgcat
     2101 tgatgaaagg cattggagaa agtgctttgc cattttacat cgtaaccatc aactgtaaaa
     2161 ctggatggtt cggaggaaat gggagcaatt aattttaggc aaatgaaaga ggctgtgagc
     2221 ctgccgttta acaccgttta ttcgaaaaag gggagtatga cacaactgtt gtcatactcc
     2281 ttttttcacc tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata
     2341 gcaaagaaat gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat
     2401 tagcaactat atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa
     2461 aaaacggaca aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag
     2521 ttgaaggatt cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta
     2581 aaagccgtat cggtcattac gcttttggaa tcgttactgc tacacgaaaa gatttaggtg
     2641 cgtttgtcga tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga
     2701 caatgacaga gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg
     2761 caaaagaccg tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat
     2821 atggaacaga agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa
     2881 caggtacgta cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat
     2941 ataatgaacc tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg
     3001 gtgtactgaa tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa
     3061 tgattttaac cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg
     3121 atccagaaga aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag
     3181 gtaatctttt gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa
     3241 aatctaattg agaatccctt gcatgttttt cttaactagc ttataataat tttaattaac
     3301 atagtgttat atcttttttt atttgtaatt attataaatt gttggggttg agtaaatggt
     3361 catgaatgct atttcggcga tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa
     3421 gctgacaccg caaagagaag ctactctgct tgtgcttttg gaaaatgaga aagagcattt
     3481 atctgcagaa gaaatcttct ttttagtgaa aaaaaagagt ccggaaattg gacttgctac
     3541 tgtttatcga acccttgaaa tcttgacaga attgaaaatt gtcgataaag taagttttaa
     3601 tgatggatta gcccgttatg atttgagaaa agaaggcgcc aaacatttcc atcatcattt
     3661 actatgcttg aactgcggca cgatcgagga agtagaagag gatcttttac ttgaagtgga
     3721 gcaagtagta gaagaacgtt atcatttttt agtaaaagat catcggctaa cgttccatgg
     3781 aatctgtcag gaatgctatg aaaaagaaa
//
LOCUS       DAFFQY010000125         3761 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFFQY010000125 DAFFQY010000000
VERSION     DAFFQY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.126"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            514..1554
                     /locus_tag="LRB02_002479"
     CDS             514..1554
                     /locus_tag="LRB02_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4660639.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2378..2710
                     /locus_tag="LRB02_002480"
     CDS             2378..2710
                     /locus_tag="LRB02_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL4660640.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3329..3670
                     /locus_tag="LRB02_002481"
     CDS             3329..3670
                     /locus_tag="LRB02_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL4660641.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1388 a          489 c          690 g         1194 t
ORIGIN      
        1 agattaacac agtagtatct tattaaataa gttaatttta atggtagtac ataattatta
       61 tatatatctg acattggtag tacttattgt attttttagt agtatataat ctattgtttt
      121 ctagcctaaa gccttgtctt ataagaataa tataatatat actactgaaa taaggtagta
      181 tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa tttatttttt
      241 gttttgacac tggtagtact taattatctt taattttaaa actaaatttt attcatacgt
      301 ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta atgactttaa
      361 aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa aaaacagttt
      421 taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg taattttaca
      481 taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt ttttaaggcg
      541 gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt ttctgacgaa
      601 tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc aagattagag
      661 atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac ttatacgaca
      721 aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat caaaaaagag
      781 ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca gttagggaaa
      841 aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga tatctactta
      901 ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg tatgaaaatc
      961 atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga gggtatgaaa
     1021 atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc agaacttaat
     1081 aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg cctacaagat
     1141 caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc tacgtttgtc
     1201 cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga agctcaaaaa
     1261 accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtgg tatttcgata
     1321 gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata cacgacactg
     1381 ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt
     1441 gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa
     1501 cgtttgaatt atgaaagctc cccaagcact attacgaaag actggttaga gtgaaacttt
     1561 actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt tttattattc
     1621 taattatatt acgaatatta caaaataata gctattttaa tagaatttat aagttaaaat
     1681 attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag aaataattaa
     1741 tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag ttataataat
     1801 acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat tacttctttt
     1861 caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt ctgctatgct
     1921 tgtgatgaat tgattttcgt tctgcacttt tggatgataa atttggacaa ttgaaaatac
     1981 aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc gttcatacac
     2041 ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt acccgcaatg
     2101 gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt taaggttaaa
     2161 aatacgtgat attacgatga atctatgcct aagcacaatg ttataaaaat gtccgtgtgg
     2221 ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat gcgttccctc
     2281 ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac aagaaaaact
     2341 tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag gaattttgag
     2401 tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa
     2461 actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa tccagaaaga
     2521 caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa ttttaaaaga
     2581 tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc aaaacggtgt
     2641 actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga tggggcgttt
     2701 gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct tgcttgaaaa
     2761 tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt agacttgatc
     2821 tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta acgtaaaaaa
     2881 ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg tgtaaataat
     2941 aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag cgtctagatt
     3001 aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa taaggagtag
     3061 gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga
     3121 tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct actagggttt
     3181 ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa
     3241 cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt gatataaaac atagagaaaa
     3301 tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata tacgattggg
     3361 cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag
     3421 aagaggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga
     3481 tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg atgaaggtaa
     3541 tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac
     3601 ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg tagcggataa
     3661 agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg agtcacgatg
     3721 attaataaaa aggtagtatt accttaggca ttgagtaagg a
//
LOCUS       DAFFQY010000126         3524 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFFQY010000126 DAFFQY010000000
VERSION     DAFFQY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.127"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(216..503)
                     /locus_tag="LRB02_002482"
     CDS             complement(216..503)
                     /locus_tag="LRB02_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL4660642.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(493..822)
                     /locus_tag="LRB02_002483"
     CDS             complement(493..822)
                     /locus_tag="LRB02_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4660643.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            1127..1456
                     /locus_tag="LRB02_002484"
     CDS             1127..1456
                     /locus_tag="LRB02_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660644.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(1743..2228)
                     /gene="vanZ"
                     /locus_tag="LRB02_002485"
     CDS             complement(1743..2228)
                     /gene="vanZ"
                     /locus_tag="LRB02_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL4660645.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2381..3292)
                     /locus_tag="LRB02_002486"
     CDS             complement(2381..3292)
                     /locus_tag="LRB02_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL4660646.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1137 a          618 c          507 g         1262 t
ORIGIN      
        1 actaaaaaac aatataaaat aacgcatttt aagccctctt tgtgcataag gatgaagctt
       61 ttaatttgaa agaggcttaa aatgcgtttt aattttttct atttttcata aatatatagg
      121 ttagtggttt aaaattatag ggtattacaa ctctcagctg ttaaaaatta tcttttaaag
      181 tgtcgtctat cctctcaagt tgaaacattg ggttcttacc taatcttttt taattgttca
      241 taaaaatttt catgcgttcc acatagtaag ataggtatca ctgcattatc tttaatttca
      301 tatgcaacgc gatatgtcgt acctgcatac ttgaatccaa tagcccaaat tcctgataaa
      361 tcacccgttt tttgttctcc cgaatgagga cgaactgcta tctcatcata aattatcgtt
      421 aaaaattgtt cttttaatgt tttctctttt aattttttta gatactttat tacgtgttta
      481 gaaggtagaa tctcataagc caatttctgc tgccaactcc tcttttgtca tcggtttaga
      541 atttgcaaca gtatcttcag caatagattt aaatgcagaa gttaacgcat tttttctatt
      601 tctaaattct tttaagatct cttgtttgct ataaccttct gaaagaatat cagccaaaat
      661 atcttcacta aaatcaaaaa attctttttt aggctctaca aactcataaa ggataccatt
      721 ctctacaagc ttagcttcta cttcaacacc attgggcaca tcgaattctt tagggaccgt
      781 taacataata gaatttcctt gttttcttgt ttttgtaatc atattatttc ctccattatt
      841 ttacatttcg taattactta gtaattacat tgtacgctat ttttagtaga aatacaaaat
      901 aataaaactc ctttagaaca attttcaaag gagttttatt tgattaggaa attatgtaga
      961 ataataatat gtttttaact tggaatcaaa aacgcacttg caagtaagtt tacttcaata
     1021 catgaaattg cttataatag tgtattggaa ttaaggtaga ttgacaaaag agtgatcaaa
     1081 taggtatact tcattcaaat aatcgtttga atgtgaggtg agatgaatga aaaaagtttg
     1141 ccaagtgact gtcgtacata aagaaaaagt aaatggaata aaaaataaat tggatcaaaa
     1201 agatttttca aacttacttg tgttagggaa atgtttcagc gatttttcta gaattaaaat
     1261 tttctatgct ttagaaacct ataaggaaat gtgcgtctgt gatttagcag aaatacttac
     1321 tgctagtgtc gctacaacgt cacaccattt acgtttttta aaaaaacatg gaatggcaaa
     1381 atcacgtcaa gatggaaaag tagtttatta ctctttagcg aatgaagaca tcctttccgc
     1441 tattcgtgtt ttttaaagtt atcagaaaat ctatctatga aatcttagtt tgggggtacc
     1501 gtcaggaaaa tgcggattta caacgttaag gaaatttatt aaagagacca attcctcagc
     1561 actaaaattg ataattgggc tttctttgtt actatagtgt taggtaagct ttttacttta
     1621 tcaccggttt tcccctcact tcacacctac gggcgagttt ccccacatcc cgttttccat
     1681 ctaatccaat tcattcaata tctttatgtg tatgaaagat cagattgctt gatttagaca
     1741 tcttacacgt aatttattct taaatgggta cggtaaacga gcaataatac gagcaaaagt
     1801 atacctacaa aaataataac tctgtctaat tttttttgat tcatatgctt attgcttaaa
     1861 ccatataatt tcagtccaag aaagcctcca acagtatttg taattacatc tgttatgtct
     1921 gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa aaccagtaca
     1981 aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag caagccaaat
     2041 ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc agtaaatgga
     2101 gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta ttgtaatttg
     2161 aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct agataatatt
     2221 tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt atagcaatgt
     2281 cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg ctagtacaat
     2341 aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt gaattagtat
     2401 gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct gatatctcat
     2461 aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa taaaagatct
     2521 catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg taatccatat
     2581 attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa ccaacatagc
     2641 gaatatgcca tggttcatat tgaattcctg ttaactctgt tttgtcctct ggataacgta
     2701 aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg gctcgttcca
     2761 ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca ctataacctg
     2821 ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca tcaaagtctc
     2881 gatagccact attaataata aaatgactaa cgccaccctt tacagcatca ttgaccatct
     2941 ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc aacccgtatc
     3001 catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca ctttcttggc
     3061 gaacaggata tttactattg attaatagca gatttccttg ataaacctgt tcttctgtaa
     3121 ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct ttgggatttt
     3181 gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta acgtagtcat
     3241 aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc attttcagtc
     3301 tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc cttaaacggc
     3361 acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt atttcgccct
     3421 actggattta ctattatcac caatgtagat gaattttcat aagtgatacc catgctgaat
     3481 ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga aata
//
LOCUS       DAFFQY010000127         3517 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFFQY010000127 DAFFQY010000000
VERSION     DAFFQY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.128"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            179..376
                     /locus_tag="LRB02_002487"
     CDS             179..376
                     /locus_tag="LRB02_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660647.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            482..964
                     /locus_tag="LRB02_002488"
     CDS             482..964
                     /locus_tag="LRB02_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660648.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1022..1618)
                     /locus_tag="LRB02_002489"
     CDS             complement(1022..1618)
                     /locus_tag="LRB02_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4660649.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            2004..3470
                     /locus_tag="LRB02_002490"
     CDS             2004..3470
                     /locus_tag="LRB02_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL4660650.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         1044 a          578 c          705 g         1190 t
ORIGIN      
        1 attttttgaa aaaaattctt ctaaatacaa cagctgtttt aattgtgaaa acgaaaacag
       61 tacgctataa tgcctgtaag gttgattttt aactgtaaat aaaaatcaat gagttaaatc
      121 tccaaagaaa tagcagtggt aatgtggaga aaagttccag cagtttagga ggattttgat
      181 gacggaagaa atgattaatt taggagaaca atatgcatgc aaaccaattg gttttacaaa
      241 aacggtcatt ggtgaagttg tatcaaaaat gacaaactgc gccgtggtca aagttgccca
      301 atgtgctgca gaggaccaag aattattaga tgaaaaagca agcatggttg ttgcaaaata
      361 tgatacattt gaataaagaa aacaattgga cgtgcggcgt gctgcaagtc caatttcttt
      421 gagaaggtaa atcagtattc ttttcctatt ttctggagaa gaaaaagcga ggcgagttgt
      481 catggtaaca ttctttacgg ttctattgtt tttattcgtt gttgtgatgt ttttatttct
      541 ttggctggtt cgaaaagaaa ttatttatcg gactgtgcga aatcgatggg tctacttggt
      601 gatacccttc ttagtgtcac tagtcatttg gtatacactc atcagtcatc ctacagcaga
      661 tgaattggca aaagggatct tgtcagcaat gatttttatt agttttttgt tggacagtag
      721 aggaatcaca gaagaaggac ttgttttgaa tagctttgac aaaaaaggtg ttcctttttc
      781 tgagatcaat aaaattgtat tatatcagcc aaaaggttct aaaatcgtaa aaatgaattt
      841 tttccgaaac ggatggcgag gaccgatgct gaaattttct gcatcattag aagaattagt
      901 tccatttttg tcccaacgct taaacgatga ggcagagatt gatattatga ttgatccaga
      961 ataaaaacag aggttgcgac aagatgtttg tcacaacctc tgttttttat ttcaaataaa
     1021 attatccaaa tacgcggaag cgatcggcat cgttcatgta ttctttttcc ccagccggtg
     1081 tttctggtga accgaatact aattgcgaac gtagtttcca gtttgaaggg atattccatt
     1141 cttttgcaac tgcttcgtca ataactgggt tgtaatgttg caagtttgcg cccaatcctt
     1201 tatcaactaa tgctacccaa gtattagctg ttgcgattcc gtttgattgt tctgaccaat
     1261 ctgggaagtt gtctgcatat aattcaaatt gttcttgtag accttttacc acgtcagtat
     1321 ctttgaagaa taatactgta ccataaccat ttttgaaacc agctaatttg ttttgtgtat
     1381 ttgggaaagc ttctgctgga gtcaatgggc gaagtgcttc ttcagtgatt tcccaaagtt
     1441 tttcatgtgc atctccgaaa aggataactg cgcgtgttga ttgagcattg aaagcagttg
     1501 ggctttcttt aattgcttct ttgatcaaag ttgttaattc ttcattagaa agtgaaacat
     1561 tacgtcctag atgatagatt gaacggcggt ttttcaaagt gtcagtaaaa gttgtcataa
     1621 taaaatttct cctttgattt gcgttttgca tttttttaat tgattacgaa atgaagtata
     1681 gcaccttaca aaaagtaagt aaacagatat gctcacaaaa aataagcgaa gttcaaaaaa
     1741 caattttaat aagaaaaatc gttatgtaag gattttctta gttatcgctt tcattgttgt
     1801 gttttaggaa tttttgctta tttgaaaaaa acagctgata tataattatt aaatctctat
     1861 cttctttttt tatgatattg ccattaaaaa ttacttgctt ttatagaaaa ttagtttata
     1921 tttcttaaat aagtttttta gttaaatttt tcggtaaaga tgtataatat tttctgaaaa
     1981 tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc
     2041 cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta
     2101 tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga cttggattac
     2161 cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta
     2221 tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg
     2281 gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt
     2341 ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca
     2401 tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt
     2461 acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag
     2521 aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac
     2581 aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt
     2641 tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg
     2701 ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata
     2761 caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa
     2821 aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag
     2881 caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg
     2941 aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga
     3001 atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg
     3061 aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt
     3121 acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt
     3181 ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt
     3241 tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt
     3301 tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg
     3361 gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc
     3421 tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca
     3481 taaaaacagg aattgcttat ttgcagcagt tcctgtt
//
LOCUS       DAFFQY010000128         3416 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFFQY010000128 DAFFQY010000000
VERSION     DAFFQY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.129"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(818..1159)
                     /locus_tag="LRB02_002491"
     CDS             complement(818..1159)
                     /locus_tag="LRB02_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL4660651.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1159..1389)
                     /locus_tag="LRB02_002492"
     CDS             complement(1159..1389)
                     /locus_tag="LRB02_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4660652.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1676..2014
                     /locus_tag="LRB02_002493"
     CDS             1676..2014
                     /locus_tag="LRB02_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660653.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2219..2458)
                     /locus_tag="LRB02_002494"
     CDS             complement(2219..2458)
                     /locus_tag="LRB02_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL4660654.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2434..2781)
                     /locus_tag="LRB02_002495"
     CDS             complement(2434..2781)
                     /locus_tag="LRB02_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL4660655.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1075 a          639 c          536 g         1166 t
ORIGIN      
        1 agaaggtatt gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaacttaca
       61 ttatactgga atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg
      121 taatcggtaa agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat
      181 cataaaaggt acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc
      241 tgacccaatt gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa
      301 aaactagcgg tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg
      361 caaatttctc ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt
      421 aaactataga aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc
      481 gatatagttt aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac
      541 tgtcagttgt tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta
      601 agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca
      661 cctcaatcat tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc
      721 atcgaccaca ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt
      781 tagcatataa aaatattata gagtttactt acaatgctta aaacatatac tgtataatct
      841 gatcaatttt agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct
      901 taaaatcaag agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag
      961 gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa
     1021 acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa
     1081 tttcttttcc tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat
     1141 aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc
     1201 gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac
     1261 taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt
     1321 ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt
     1381 aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa
     1441 attccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata
     1501 aatagaaata aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac
     1561 attgaaccta tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa
     1621 ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc
     1681 cgattataaa caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt
     1741 cacgaaacta gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat
     1801 agaaacattg gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat
     1861 cgtagctatt tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa
     1921 atggcaacct cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt
     1981 gaattttaga ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa
     2041 aaaagactcg cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt
     2101 gaccctatag atgcagtata tgcagcttat gagtatttct tctgttctct ttgtttttgg
     2161 gatctgtgct gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt
     2221 ataccttctc cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa
     2281 tatctgttac ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga
     2341 ttggtgcgac tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat
     2401 cctgatggct acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa
     2461 atttgtcttt ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg
     2521 atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt
     2581 tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt
     2641 tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt ccgctgcctg
     2701 cttcggttgt ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc
     2761 aaaaaaataa cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca
     2821 acaagaagtt cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca
     2881 agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat
     2941 attcaacaaa catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg
     3001 caggcttacg acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa
     3061 tttatgcaaa aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc
     3121 gactttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc
     3181 ccaaccaaac gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca
     3241 gggcagcttt ttcacaacct ctccactaaa cgatgttcaa tcagctaacc ttcggtatta
     3301 gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt
     3361 caaagcgctg atttcacaac ctctaataca cggtgttcca gaagtagctt cttcga
//
LOCUS       DAFFQY010000129         3403 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFFQY010000129 DAFFQY010000000
VERSION     DAFFQY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.130"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(75..1073)
                     /locus_tag="LRB02_002496"
     CDS             complement(75..1073)
                     /locus_tag="LRB02_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL4660656.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LRB02_002497"
     CDS             complement(1148..1444)
                     /locus_tag="LRB02_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL4660657.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LRB02_002498"
     CDS             complement(1437..1730)
                     /locus_tag="LRB02_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660658.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LRB02_002499"
     CDS             complement(1876..2427)
                     /locus_tag="LRB02_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL4660659.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LRB02_002500"
     CDS             complement(2601..2900)
                     /locus_tag="LRB02_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL4660660.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="LRB02_002501"
     CDS             complement(2927..3193)
                     /locus_tag="LRB02_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL4660661.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          993 a          669 c          461 g         1280 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atc
//
LOCUS       DAFFQY010000130         3389 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFFQY010000130 DAFFQY010000000
VERSION     DAFFQY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.131"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            646..963
                     /locus_tag="LRB02_002502"
     CDS             646..963
                     /locus_tag="LRB02_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL4660662.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1054..1419)
                     /locus_tag="LRB02_002503"
     CDS             complement(1054..1419)
                     /locus_tag="LRB02_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660663.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1627..2109
                     /locus_tag="LRB02_002504"
     CDS             1627..2109
                     /locus_tag="LRB02_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660664.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2306..3196
                     /locus_tag="LRB02_002505"
     CDS             2306..3196
                     /locus_tag="LRB02_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL4660665.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1079 t
ORIGIN      
        1 tattgctttt gcaaaattta tttacctgtt ttctggatca acgatagtgt acattatgct
       61 atccttgttt ggattttagg acgtttaaag aggcactttt ttacataact taatccatag
      121 accttttaag catgctgtga gataataata aagaagagtt taaagcgcac cccaaaccac
      181 ttccccataa gtgtattacg ctttaaactc ttttatattt gaattcacta agaagcatac
      241 catatatttg aatttactaa gaagcatacc atatttttga aaaaaagtga gaaaaaaggc
      301 ttataattag gatttagagt aattaattag atgtttattt tataggactg atactataga
      361 ttacaaatag tatcgtattt cgcaatctta aaattatctt cttactgtga ataagataat
      421 aaaatttgta tttgtccttt ttaggtaact tattttaatc tatatacctt tctggatcaa
      481 cgaaagtata ctttatatag tcataacgcc gatggtaact atgcactgta acgataatgc
      541 tgctaaaata tctttttacg aaaaacatca caaggaaaaa cttaactatg gtattatcta
      601 aacataattt tttcggtatt taatatttaa ttaggaggta atattttgct tttttatagt
      661 aattttatct taatagtagc gatattatta cttctgaata tatggatttt tgataggagt
      721 agaaattcaa gtataggttt tagaactaaa cgaagtttgt cttctaaaaa aaattgggta
      781 tactcacaaa ctatttttta tggaggaatt gttttaatta gtttactttc ttcaacactt
      841 tattctttga atattataga tgtttccacc tcaaattcaa tatctattat tggaatcatt
      901 attgctgcaa ttattacaca actgttttta gtgtttggag agaagaaaag aagtaaaaaa
      961 tagaaaaact agtgatagtt ttggtgatat aagaaaaaca tatatgtata aaaagaagaa
     1021 agtagcttct ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga
     1081 taaggctacc gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag
     1141 caagatcagc tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc
     1201 gcaaaataaa taggaagtga cagtagatat aatattggca atgaataaag atataatacg
     1261 cctgttcctt tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc
     1321 gcttacggaa tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga
     1381 taaaagcaac ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag
     1441 tgttattatt ttgttagttt tattgtaaat tgaatcctgc aataatcaat tactttttca
     1501 ttatttctag attaaatgaa taaactctca tgccattatc tctaaaaaaa aagtttttcc
     1561 ccctaatttt tttagataga aaattgtata gtatgatgag agaaaaagta gtttggggga
     1621 acgttaatga gaagaaaagc agtaatcgat cggctttatt tatttttttg gttttttcct
     1681 atcgttttgc taccttttac atgggtaaat gctgttgtac aaggtgcgga gatattaggc
     1741 ctgatccaag gaagcggatt tgtggtttct aggaattttc cagctttgat tgttttctat
     1801 ttagtgataa gtgtaggtct cttattacca aaatctctga tcaatcacta caattggcag
     1861 ctgctttact tttcttgctt attagtattt acatctatgt tttctttgct gcctcgcttg
     1921 attatcggcc ctaatggttt gagcgagagt ggattgaagg atttagctta ttcctccatg
     1981 tatttcttga cgatcaaacc atggttctat ttagtaattg cctcatttat tcttgcaaca
     2041 tttttttact tctggacaga aatcaggata aaaagtgaac gatctgtatc tataaaaaat
     2101 aactattgat ggtggatcat cagtaaaaaa acatatgagc tgcgtaaaat gcatgtttct
     2161 catatgcttt ttttattatt atgtaaagaa tgcgaaaaac gtttacatga tttttacata
     2221 acttaactat atttttacag caaccatacg agggatttac agtcgactga taaactttag
     2281 ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg tttactctag gtatgatggg
     2341 aattctttta gccggatgcg gctcaggatc aggaaataca gatggctctg ccacacaaaa
     2401 taattcaagt gactcaaatg aacaagtaaa aatcgttgcg gtaggttcga cagcattaca
     2461 gccgttagtc gatgcagcac aagaaagctt tgtacaagaa aatccaaatt atcaaatctc
     2521 tgtacaagga ggaggaagcg gaactggttt gagccaagtc gaagctggag cagttactat
     2581 cggaaactct gatgtatttg cagaagaaag agacggagtt gatgcttcaa aattagtgga
     2641 tcataaagta gcagtggtcg ggatggcacc aatcgtcaat aaagatacag atgtcaaaga
     2701 tatcaccaaa caggaattga ttgatatctt tacaggaaaa atcactaact ggaaggaagt
     2761 tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca aacggcagcg gtacccgcgc
     2821 gacattcgaa aaatggggac tggatggtgc tacccctgtg cagtcccaag aacaagattc
     2881 atcaggaaca gttcggcagc tggtaagtca aacaccagga gcaatcagct atttagcttt
     2941 ctcatatctc gatgattcca cacaagcact aagtatcgac ggtgtagaac caaaagaaga
     3001 gaatgtagca gataacagct ggggaatctg gtcatatgaa catatgtaca cgaacggaaa
     3061 accttcccct gaagtccaaa aattcttaga ctacatgatg acagaagaaa tccaagaagg
     3121 acctgtcaaa gaattgggct atttaccaat cacaatgatg gaagtagaac gtgatcacga
     3181 aggaaatatc aaataactag cagataaaca tgccacggta gtcgttacta ttaaggaaaa
     3241 atcaactatg tttccccaca tatacatttt tcctttaatt gtgaaaaata gttcctcata
     3301 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tattcgaaaa
     3361 aaagagctac gacatagaac tgtcgtaac
//
LOCUS       DAFFQY010000131         3378 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFFQY010000131 DAFFQY010000000
VERSION     DAFFQY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.132"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(53..2545)
                     /locus_tag="LRB02_002506"
     CDS             complement(53..2545)
                     /locus_tag="LRB02_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL4660666.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="LRB02_002507"
     CDS             complement(2552..3019)
                     /locus_tag="LRB02_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL4660667.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3155..3228)
                     /locus_tag="LRB02_002508"
     tRNA            complement(3155..3228)
                     /locus_tag="LRB02_002508"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3192..3194),aa:Arg,seq:acg)
     gene            complement(3286..3359)
                     /locus_tag="LRB02_002509"
     tRNA            complement(3286..3359)
                     /locus_tag="LRB02_002509"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3323..3325),aa:Pro,seq:tgg)
BASE COUNT          921 a          747 c          556 g         1154 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt cgaaccccta
     3181 accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat attcaattta
     3241 aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat
     3301 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
     3361 ttaaaaaata aaaaagac
//
LOCUS       DAFFQY010000132         3351 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFFQY010000132 DAFFQY010000000
VERSION     DAFFQY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.133"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..238
                     /locus_tag="LRB02_002510"
     CDS             <1..238
                     /locus_tag="LRB02_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660668.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="LRB02_002511"
     CDS             252..440
                     /locus_tag="LRB02_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660669.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="LRB02_002512"
     CDS             452..1063
                     /locus_tag="LRB02_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL4660670.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="LRB02_002513"
                     /pseudo
     CDS             1048..2439
                     /locus_tag="LRB02_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2655..2957
                     /locus_tag="LRB02_002514"
     CDS             2655..2957
                     /locus_tag="LRB02_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660671.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="LRB02_002515"
     CDS             2950..3297
                     /locus_tag="LRB02_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660672.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         1307 a          473 c          654 g          917 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 taagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca t
//
LOCUS       DAFFQY010000133         3339 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFFQY010000133 DAFFQY010000000
VERSION     DAFFQY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.134"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(133..2130)
                     /gene="tkt"
                     /locus_tag="LRB02_002516"
     CDS             complement(133..2130)
                     /gene="tkt"
                     /locus_tag="LRB02_002516"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL4660673.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2232..2483)
                     /locus_tag="LRB02_002517"
     CDS             complement(2232..2483)
                     /locus_tag="LRB02_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL4660674.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2629..3252
                     /gene="lexA"
                     /locus_tag="LRB02_002518"
     CDS             2629..3252
                     /gene="lexA"
                     /locus_tag="LRB02_002518"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL4660675.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          903 a          733 c          623 g         1080 t
ORIGIN      
        1 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
       61 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      121 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      181 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      241 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      301 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      361 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      421 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      481 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      541 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      601 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      661 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      721 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      781 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
      841 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
      901 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
      961 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1021 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1081 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1141 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1201 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1261 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1321 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1381 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1441 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1501 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1561 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1621 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1681 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1741 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1801 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     1861 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     1921 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     1981 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2041 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2101 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2161 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2221 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2281 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2341 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2401 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2461 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2521 aaactgaaaa aaaacaacta gataaactaa ataaaatacg aacattcgtt tgatttagcg
     2581 ctttttatat ggtactattt atttatcaag tgaaagaagg tgtaccttgt ggcccgacaa
     2641 actgaatcaa gacaaatcga agtcttacga tatatccatg aacaagtaac ccaaaaagga
     2701 tacccgccga ctgtccgtga aatcggggaa gctgttcagc tttcttctac ttctactgtt
     2761 cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc aaagagatcc gacgaaacct
     2821 cgtgcaatcg agttgacaaa acaaggttta gaaaagattg ggattcgtcc aacaactatt
     2881 ccagtacttg gcgtcgtaac agctggagaa cccattttag cagtagagga tgcttcagac
     2941 ttctttccaa tccctcctaa tctttcttcc gaagaaggca gtctctttat gctgacaatt
     3001 cgtggagata gtatgatcaa tgctgggata ttggatggtg acaacgtgat cgttcggaag
     3061 caagaaactg ctcaaaatgg cgatattgtc attgcaatga ctgccgagga cgaagcgact
     3121 tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc agcctgaaaa cgacttgctc
     3181 gaaccaatca tcttagatca agtatctata ttaggacgag tagtaggact ttatcgtagt
     3241 catatctatt aattgctact aattaaaaga aaacttttta aaaagagttt gagacgttgg
     3301 tctcaaactc ttttttcgcc cgggttcttc ttctttcaa
//
LOCUS       DAFFQY010000134         3295 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFFQY010000134 DAFFQY010000000
VERSION     DAFFQY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.135"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            114..1103
                     /locus_tag="LRB02_002519"
     CDS             114..1103
                     /locus_tag="LRB02_002519"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL4660676.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="LRB02_002520"
     CDS             complement(1420..1944)
                     /locus_tag="LRB02_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL4660677.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3295
                     /gene="scm"
                     /locus_tag="LRB02_002521"
     CDS             2231..>3295
                     /gene="scm"
                     /locus_tag="LRB02_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL4660678.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1049 a          667 c          611 g          968 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DAFFQY010000135         3232 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFFQY010000135 DAFFQY010000000
VERSION     DAFFQY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.136"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            184..1138
                     /locus_tag="LRB02_002522"
                     /pseudo
     CDS             184..1138
                     /locus_tag="LRB02_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
     gene            1155..2179
                     /locus_tag="LRB02_002523"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="LRB02_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="LRB02_002524"
     CDS             2185..2664
                     /locus_tag="LRB02_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL4660679.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3232
                     /locus_tag="LRB02_002525"
     CDS             2680..>3232
                     /locus_tag="LRB02_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL4660680.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          980 a          539 c          751 g          962 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cg
//
LOCUS       DAFFQY010000136         3167 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFFQY010000136 DAFFQY010000000
VERSION     DAFFQY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.137"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="LRB02_002526"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="LRB02_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL4660681.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="LRB02_002527"
     CDS             complement(1258..3099)
                     /locus_tag="LRB02_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL4660682.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
BASE COUNT          827 a          694 c          561 g         1085 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggta
//
LOCUS       DAFFQY010000137         3018 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFFQY010000137 DAFFQY010000000
VERSION     DAFFQY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.138"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(350..1390)
                     /locus_tag="LRB02_002528"
     CDS             complement(350..1390)
                     /locus_tag="LRB02_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4660683.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1739..2065)
                     /locus_tag="LRB02_002529"
     CDS             complement(1739..2065)
                     /locus_tag="LRB02_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660684.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2052..2855)
                     /locus_tag="LRB02_002530"
     CDS             complement(2052..2855)
                     /locus_tag="LRB02_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4660685.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          905 a          499 c          414 g         1200 t
ORIGIN      
        1 actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat
       61 atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact
      121 caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat
      181 atctaacgat tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta
      241 aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa
      301 aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc
      361 aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct
      421 ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca
      481 aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc
      541 ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta
      601 attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt
      661 aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac
      721 tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag
      781 tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg
      841 cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt
      901 tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa
      961 ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta
     1021 cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat
     1081 ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag
     1141 atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa
     1201 atatattatc atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa
     1261 gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa
     1321 ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg taataattaa
     1381 aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa
     1441 tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac
     1501 tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa
     1561 gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat
     1621 attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac
     1681 tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt
     1741 aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg
     1801 actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg
     1861 tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta
     1921 cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt
     1981 ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag
     2041 cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta
     2101 aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca
     2161 gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca
     2221 ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc
     2281 ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt
     2341 accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca
     2401 actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt
     2461 ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag tactctttta
     2521 agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg
     2581 gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct
     2641 tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct
     2701 gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc
     2761 aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct
     2821 actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata atataattat
     2881 atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta
     2941 ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat
     3001 taaaataatc acttttta
//
LOCUS       DAFFQY010000138         2896 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFFQY010000138 DAFFQY010000000
VERSION     DAFFQY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.139"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            49..1437
                     /locus_tag="LRB02_002531"
     CDS             49..1437
                     /locus_tag="LRB02_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL4660686.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>2896
                     /locus_tag="LRB02_002532"
     CDS             1775..>2896
                     /locus_tag="LRB02_002532"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL4660687.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRK"
BASE COUNT         1072 a          444 c          524 g          856 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaa
//
LOCUS       DAFFQY010000139         2849 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFFQY010000139 DAFFQY010000000
VERSION     DAFFQY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.140"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            204..1172
                     /locus_tag="LRB02_002533"
     CDS             204..1172
                     /locus_tag="LRB02_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL4660688.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="LRB02_002534"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="LRB02_002534"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL4660689.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="LRB02_002535"
     CDS             2202..2810
                     /locus_tag="LRB02_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL4660690.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          844 a          545 c          693 g          767 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa actggttctg
     2821 ttgcaaagtt ttaaataaag aataaaatc
//
LOCUS       DAFFQY010000140         2784 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFFQY010000140 DAFFQY010000000
VERSION     DAFFQY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.142"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1023
                     /locus_tag="LRB02_002536"
     CDS             <1..1023
                     /locus_tag="LRB02_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4660691.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="LRB02_002537"
     CDS             1157..2500
                     /locus_tag="LRB02_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4660692.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="LRB02_002538"
     CDS             2612..>2784
                     /locus_tag="LRB02_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660693.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          517 g          795 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctgggaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DAFFQY010000141         2778 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFFQY010000141 DAFFQY010000000
VERSION     DAFFQY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.143"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..197
                     /locus_tag="LRB02_002539"
     CDS             <1..197
                     /locus_tag="LRB02_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660694.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="LRB02_002540"
     CDS             314..811
                     /locus_tag="LRB02_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL4660695.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="LRB02_002541"
     CDS             898..1290
                     /locus_tag="LRB02_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4660696.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="LRB02_002542"
     CDS             1274..>2778
                     /locus_tag="LRB02_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660697.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DAFFQY010000142         2652 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFFQY010000142 DAFFQY010000000
VERSION     DAFFQY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.145"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            712..2571
                     /gene="ltrA"
                     /locus_tag="LRB02_002543"
     CDS             712..2571
                     /gene="ltrA"
                     /locus_tag="LRB02_002543"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4660698.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1026 a          421 c          508 g          697 t
ORIGIN      
        1 aaaaggttag gcacttaaaa gaagaaaaca taagtttcct tttaaggaga actgatattt
       61 tgagcagtgg aatgatgggg taacgccctg aaacgctccc ttaatactcc gactggctgt
      121 ttaacatata gctgttagat ggtcagaagc tcggtgaagt cggctgaaag ttaccgtaaa
      181 tatgataaat tcatatacga aagttgttca gaggtgaatg atgtaaccta taggtcggag
      241 gtctataaaa tactcacggt gagaatgtaa gtaatactac tgacgaactt gcgaatgaaa
      301 gaatctatac acggactgta tagaaatgta caggattcta ccgtatcaac ggaacagaat
      361 gtatgttgtg gtttagtaag attattggtt atgaacaacc atgcaatgtt acaggcattg
      421 gaaatataca ggttcaaagc aagcacctaa aagtatatat aaagataaaa atatcgaaac
      481 atgggaagct ggcaatgtgg aggaaactat attccattga aacatttgtg acgaagaata
      541 ataagcacat ttatgccagt gaaagtggtg gcacagtacc aatgaggata tagaaatata
      601 tcggaggaat agccactagt caaaagaaat cgttaattaa atgatgtatt tcatagaatc
      661 gagtaagaca aaataaacac tacaacctaa gtaaacaaag gtagtggtgc tatgaataaa
      721 gtaaaaacaa ataaaacact tcgtcatagt gaatactatg gcatgacaca aatatttgat
      781 gaactgtatg taaaaagtga taaaaaagaa gtttttacag agctcatgac aattattatg
      841 agtgatgaaa atattcgact agcatacaga aatatcaaga ataacaaggg cagttataca
      901 gcgagtaccg ataagaaaag tattcgagac attaaaacac tgtcagaaga tacctttcta
      961 actgaggtta aagttctttt tcgctgttac aaaccacgag cagttagacg tgtagaaatt
     1021 cccaaaccaa atggaaaaac acgccctctg ggcattccat caatctggga taggttaata
     1081 caacagtgta ttttacaagt cttagaaccg atatgcgaag ctaaatttca caattatagt
     1141 tatggatttc gaccaaaccg ttccacacat catgcgattg ctacctgcaa tcgactggca
     1201 aataggggga agctacactt tgtagtagat gttgatatta aaggattctt tgataatgtc
     1261 aatcactcta aacttttgcg tcaaatgtgg acgattggaa tccgtgataa atggctatta
     1321 ggtattattc gatccatgct aaatgctcct atagtcctac caaccggtaa agtcgaacac
     1381 cctaaaaaag gtgtacctca aggtggtgtc ctatcccctt tactatctaa tatcatattg
     1441 aatgaattag attggtgggt tgcttcacaa tggcaaaata ccacgattga gacagaaaag
     1501 attatggata ggcgagataa aggcaaaggc attgataaga ctaatcagta tcgggcatta
     1561 agacgaacaa acttaaaaga aatatacatt gttcggtatg ctgacgactt caaaatcttt
     1621 tgtagaaaac gaagtcatgc agataaaata tttcacgcaa cgaaaatgtg gcttaaagaa
     1681 cggttatcac ttgaaattag cgaagaaaaa tccaaaattg tcaatctcaa aaaaggtaaa
     1741 tcagaatatc taggatttga attaaagcta atcaagaaag gtaaaaaata cgtagtcgaa
     1801 agtcatatga gcaaaaaatc aatgacaaga gttaaaatcc aattaaagaa acaattacgt
     1861 aaagttgcca gaccgaagaa tcattgcgaa caagcaaaag aaattggatt atataattct
     1921 atggtgattg gaatccatca atattatgga attgcaacct gtgtaaatct cgattgcagt
     1981 aaaatagcct atacactaaa accgttcatc aatcacaaac tgccgacaaa gaaacatgga
     2041 aagatattaa atacatttat caaatcgaag tatggaaaaa gtaaagaaat gcgatggttg
     2101 aataatgtgc caattgtacc gctcaactat gttcagttta gacttgctgt ccccctatca
     2161 gagggaactt gcaagtacac tgaaagtgga cgaagcaaga tacacagtaa attaaaatta
     2221 gacctagccc ttttacttca catgatgaga aatagtaatt atgaaagaag tattgaattt
     2281 gtggataaca gaatatctaa atattctgct caaaagggaa aatgtgctat cacgggtaaa
     2341 tttctcgaat acgaagaaat ccattgccac catatcgtac cagtaaaaca acagggtaca
     2401 gacaaataca gcaacctcat tattattcac aaaaatgttc acgcattagt ccatgctatc
     2461 gaagaaaaaa tcatacacaa atacctgaat gtattaaatc taaccaatga acaaatagaa
     2521 aaactaaatg aattacgtgt caaagctggt aatagtgttt taacagttta acttgaaaaa
     2581 tatatcatac ttaacattct tttgatggaa cgccgtatga agtgaaagtt tcatgtacgg
     2641 tgtgaagcgg gg
//
LOCUS       DAFFQY010000143         2650 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFFQY010000143 DAFFQY010000000
VERSION     DAFFQY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.146"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            36..197
                     /locus_tag="LRB02_002544"
     CDS             36..197
                     /locus_tag="LRB02_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660699.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="LRB02_002545"
     CDS             210..713
                     /locus_tag="LRB02_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL4660700.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="LRB02_002546"
     CDS             773..1162
                     /locus_tag="LRB02_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4660701.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="LRB02_002547"
     CDS             1149..>2650
                     /locus_tag="LRB02_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4660702.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          440 c          610 g          812 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactaccc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DAFFQY010000144         2623 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFFQY010000144 DAFFQY010000000
VERSION     DAFFQY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.147"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="LRB02_002548"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="LRB02_002548"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4660703.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          941 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaa
//
LOCUS       DAFFQY010000145         2483 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFFQY010000145 DAFFQY010000000
VERSION     DAFFQY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.149"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(1014..1304)
                     /locus_tag="LRB02_002549"
     CDS             complement(1014..1304)
                     /locus_tag="LRB02_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660704.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="LRB02_002550"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="LRB02_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DAFFQY010000146         2399 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFFQY010000146 DAFFQY010000000
VERSION     DAFFQY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.153"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            208..2070
                     /locus_tag="LRB02_002551"
     CDS             208..2070
                     /locus_tag="LRB02_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4660705.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="LRB02_002552"
     CDS             2070..>2399
                     /locus_tag="LRB02_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4660706.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DAFFQY010000147         2228 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFFQY010000147 DAFFQY010000000
VERSION     DAFFQY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.154"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(218..1117)
                     /locus_tag="LRB02_002553"
     CDS             complement(218..1117)
                     /locus_tag="LRB02_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL4660707.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1127..1483)
                     /locus_tag="LRB02_002554"
     CDS             complement(1127..1483)
                     /locus_tag="LRB02_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4660708.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1839..2135
                     /locus_tag="LRB02_002555"
     CDS             1839..2135
                     /locus_tag="LRB02_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL4660709.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          698 a          424 c          380 g          726 t
ORIGIN      
        1 ataaaaaatg tatattatat ttttttgtga ttatcgttca aaaaaagaat aaacgcttgc
       61 atctagtact ctgcgagtta caaatcatcc gatcaaataa acattcgctg attattttcc
      121 acaaaaaaag cagcatatcc acgataaatg gatctactgc tcttacttat ccttatgttt
      181 tacaaggtat tttcttttac acattactaa tgctgtctca atacggaacg attgtcatcg
      241 cctgttcaaa ttcctcttgc aaatgttgtg tttgataggc ttgaaaggcc ggatgatggg
      301 cacgcaagag gacacgtaat ttcatgggtt catagctatc taacagctgt tctttcaaga
      361 caaaatctcg ggtcagctgc cattcataat aataaccaaa aggtctgcag ctgtaaatcg
      421 tgtgaccgat ggatttatct tcaaagcgac gatgcgtatg gccaaaaact acttgtttga
      481 tatttgaata acggtcaagt aattctccga atgattctga tcctaaaaat gcatttaatt
      541 tgttccagcg ctcataagga gcagattgat acatgataaa tgcccgttta ggaacgaaat
      601 gagtggataa aatcacctca agtttttttg cttccagctg gtctagcaaa ttttttgcag
      661 ctgcaagcat ttctgcattg acctcgagat ctgttttccc acgctggatg aaccgatcat
      721 accaatacaa acgcttcatt cgtacgattg cctcgtaatc tttcaattcg gaaaactgat
      781 agtcgtacca gccattcatc cctaaaagaa cggtctgctc atttaaagca atataccgtt
      841 catttaaaaa atgtgggtct ggaaaatggt ccatcatctg ttcattgggc acatctgcca
      901 actcgtgatt gccgaaatga aatgtcgtag ataatccttt attgacgaaa aattcgacaa
      961 cagctaggca acggtctact ttattagccg tatctccggc tagatgaagc cgagtgatct
     1021 tgtgcttttg caaaacttgc caaagctgca tcagctctga ttcgccgaat ttcttgatgt
     1081 ccacatgtag gtcgctgatg atcgccaatt ttcccatttt atccctctag tcttttcgaa
     1141 caacttgcaa gttgcctttt ctcaagccgc ttggtccttt taattgtaaa ctattataag
     1201 ttggttctaa agcaagtgtg gatggatcat ccatgtaaag tattgcactg tctttgattt
     1261 tgattggtcc aactggtgta tcagccacta cagaataaag acttagatct gtttgtgtat
     1321 cttttttgac aatgatcaat cgaaatgccg tgtccaattg gcaactgact tggctttctg
     1381 caaatggacc gtcaccgttt tcgatatcta ataaaagttg atcgtctgct tctaatttat
     1441 tcttcaatac ttcttgtgct tcttttgtca cttctaattt catctgtatc acctcatgtc
     1501 atagaataat cactttattc atcagatgca actaatttgt ctggttctga ctgttctcaa
     1561 tttattggaa agtaagtcat tcgtttatta tgatttctct tatcacaaaa gagacaactt
     1621 tcccttttct tcgttcagtt tcttgaacgg aaaaagaccc aaaaagcaac ctgttatttg
     1681 tttaacctta ttcctttctt aaataacaaa aaatactata aaaaataaaa aacctttaac
     1741 aactattgga aaaagaatta ctttttgttc acggattatt aacaactctt cggtagagta
     1801 aacttatcaa ataaaaagac ttctttggag gaatcactat gaaggaatat ataaaaaaca
     1861 tctattttat cgaagaaaca caaaatatcg agggttctta tattgaagta aaaacactct
     1921 ttgtcaatga agataaaacc aaagcattgg atatttataa aaaattagct tcaaaaaaaa
     1981 cgaatagttt tggattgata ttaagcgagt ataaaatcaa agcagaagag agttatttct
     2041 atcaattatt aaaacggtgg agtaagcttc ccgccgattt ttatcgaaaa atgcagatca
     2101 tcaactatca accgcttgct gagacacatg cctaaataga ggttgtgaaa tcagtgcttt
     2161 gagctaatac cgaaggtcag ctgatggaac atcgtttaga agagattgtg acaactacga
     2221 tttgcttt
//
LOCUS       DAFFQY010000148         2058 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFFQY010000148 DAFFQY010000000
VERSION     DAFFQY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.156"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..590)
                     /locus_tag="LRB02_002556"
     CDS             complement(<1..590)
                     /locus_tag="LRB02_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660710.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKN"
     gene            complement(615..794)
                     /locus_tag="LRB02_002557"
     CDS             complement(615..794)
                     /locus_tag="LRB02_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660711.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(871..1380)
                     /locus_tag="LRB02_002558"
     CDS             complement(871..1380)
                     /locus_tag="LRB02_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660712.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1476..1856)
                     /locus_tag="LRB02_002559"
     CDS             complement(1476..1856)
                     /locus_tag="LRB02_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4660713.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT          578 a          420 c          325 g          735 t
ORIGIN      
        1 atattctttt gcatatgatc ataaagatga ccactgatca tccgctgacc gacaactttg
       61 aagtcatggc gttcgtataa ttttttagcc tttggatttt tttcatccac acttagaccg
      121 atgacggagc gattagcttt cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg
      181 atgccttgtc ctctaaaatc ttctgataca ctgatagagt ccaaatacca ttcgttggga
      241 aacgtttctt tatcaatgaa caaacgtgta gacgcatcta gctgatattt ctctaagata
      301 ggtaccagtg gctgatcgat gattgattct tcttcatctg tataaccgta agcaactcct
      361 gcaactttac cgtctatttc ttctacgata cctcgtgaat aactgtaacg ataatttgga
      421 tcagtgattg cttcttctaa aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat
      481 ggcagttcca tatcttttag aatcactaag atcaatgggg cgatttctgg agcatcttct
      541 tttctcgcaa aacgaatcat agtttcctct ccttttctcc attctaacat gcagaaaaag
      601 aaatcgcaaa atcgttattt taaaaaatct tcaatctttt cagtaatgaa ataaccatga
      661 gtaacgattc ttgccgcttc agttcggcaa taagggcaga cgactggcat ttctttatca
      721 ttatgttcga ctacattatt gacttcttcg tatgcaaatt tttttccgca attagggcat
      781 ttcatttttt ccatgttatc tcaacttcct ttcatcttta ctctcttagc ttactcttat
      841 attgtaaaaa agagaagcaa tatccttctt ctatgcagat atccatacct gacggtcctg
      901 ttctccatct attaatcgga ctttataata aggacgttca tctttgacta gaagtgtcca
      961 ctctttcact tttgcttttt tcccgtagta gctgactgct tccttcatcg ctgcttgcgg
     1021 ttgtttgaac tcttccgttt tttctatatc aaaaagatgt tctgcggcct tttccgtgtc
     1081 atttcgtacg gttgttttcc cagtggatgg attgactgct atgacttgtc cttcacaaaa
     1141 aaactggtat tcatatgttg cattcgtagg attctccaaa tctttttcaa ctgcaaaatg
     1201 aatagcagat atgttatgtt cttttgtttc gttttttacg attgctgctg cttttttcaa
     1261 tgagatccga tattcctggt cactaatatc atccatgtga aaagcggcat tgagcagtcc
     1321 cgtcataccg gtaatcccta ttacgatgag ggcagcaaca ccaaacatca tttttttcat
     1381 tcttcattct cctgtttctt ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg
     1441 aatgaaacga aaaaaaagtg acaattttgt tatctttatt cgtgcagttc taatggcaat
     1501 ccgtctggat caaagaaaaa cgtcattttt tttcctgtaa aggtatcttc tctaatcggt
     1561 tctgattcga tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg
     1621 aaagcaagat gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca
     1681 attccaaaaa tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt
     1741 tctttgcggt aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt
     1801 gatttctgat aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga
     1861 gcactcctat tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt
     1921 gtcagattga aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga
     1981 aagtaggtcc tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta
     2041 tattttcaag aaaacaaa
//
LOCUS       DAFFQY010000149         1921 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFFQY010000149 DAFFQY010000000
VERSION     DAFFQY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.157"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(111..1358)
                     /locus_tag="LRB02_002560"
     CDS             complement(111..1358)
                     /locus_tag="LRB02_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4660714.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1434..1580)
                     /locus_tag="LRB02_002561"
     CDS             complement(1434..1580)
                     /locus_tag="LRB02_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL4660715.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1684..>1921)
                     /locus_tag="LRB02_002562"
     CDS             complement(1684..>1921)
                     /locus_tag="LRB02_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL4660716.1"
                     /translation="DILEVLQKVYLKLEKQKYELDPGPLINRLVNYLYFTAYTNKIRF
                     TEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          688 a          307 c          208 g          718 t
ORIGIN      
        1 tactttatcg tgaaaaagaa tgaaattatc aataattcgt tctaactgat ttaattgtat
       61 aatttgatct tcacataata ttattggata actcataaat cctccttccc tcaaaacaac
      121 aaaataactt gagtagtaaa ttgatacgga tcgttttgat agttcacaaa catattggga
      181 ttctttttat tgatttcctc tatactagat aaacctaacc cttgatgtcc cttttttgtt
      241 gttattcctt ttttcattag agtatctata gagagtagta tctctctaca actattcgat
      301 atcgaaattt ctatctgttt atcatctcgg taaatagcta gagacagttc tctatcttga
      361 ctctctatag atgcttcaat tgcattatcg agaataattc caagcaccct aatacagtca
      421 aatattggaa caggaacttc aataatagta cttggacatt cgaatttaca acaaatctta
      481 ttgttatttg cttggtatag cttcgctatc aacaaacttt tcaaatattc attttttata
      541 ttttttaaat aacgatattc aaacttattg gttagataag aattagaata gatctctaat
      601 tcttttattt ctttttccaa tatcgaatca tggttttgat tagcgatttc ttttaaactc
      661 agcaataaat ttttatagtc atgtctaaat ctggcaaatt tttcttgatc tttttccagt
      721 gaatcagtat atttttttag ataaaccaat tcttttcttt tcaattgctg ttcatattgt
      781 tctcgttgcc taatagttat tctaataaat aaaaatagaa caacaaacat ttgaatgatt
      841 aaaaatatta agacaccaaa cacaaaatga tcaaatactt gatattgatg agcaacataa
      901 gaaacaaata aaccaattgt aagtaaataa atcatgaata tagcagtaag tttagaactg
      961 gactgttcaa caatcctatt tatagctaat tttctatata accaaataaa catcagcaaa
     1021 aacaaagaat caagcattat ctggaatact acataaccat acccttttat cccttcaaat
     1081 gaaaacatat acgtcataac catggaagta ataatagatc tgaaatcatc aataagcata
     1141 cataatagta atttatttaa aagcactata ttagttttct tcttttcctt taatataaaa
     1201 taacttccga ttataggtat agctgcggaa acatcagtaa ataattcaac aaaaaaagtg
     1261 agtggtatca ataaacatac aaaaagatat tttcttttgt taagatcagt attagtcata
     1321 taaaataaga gaaaaaagaa aaagtttccc acgaacattc ttaatagata agcttttgta
     1381 tcgatagtaa tcaagagatc ccctcctgat tttaggatat cataaaaaaa attttagtta
     1441 cactttttaa ataatgaaaa aacacattcc actaaatttg atgccggttt tccttgaggt
     1501 tttgttccag cacctccttt aattttttta agctcttgat ctgaacattg tttagcattt
     1561 agtctatttt tttcttccat tacgattccc cctacaaact ataaatactt ttatgtcact
     1621 gattcaatat acctattttt ctaatcaaaa aattttgaga catatttaat attataagta
     1681 tcattaaaat tgagatttat ctccataatc tgctcgatat aaaccattta ttccagcagt
     1741 tcttccaatt tcactcaaat ttcttattaa ttcctcttga tattcagtga atcttatttt
     1801 attagtataa gcagtaaaat atagataatt caccaatcta tttattaaag gaccgggatc
     1861 taattcatat ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc
     1921 a
//
LOCUS       DAFFQY010000150         1838 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFFQY010000150 DAFFQY010000000
VERSION     DAFFQY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.159"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            253..1791
                     /locus_tag="LRB02_002563"
     CDS             253..1791
                     /locus_tag="LRB02_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL4660717.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT          677 a          382 c          352 g          427 t
ORIGIN      
        1 tcttttttta tttttaggtc gaaagcgaaa aagaatcgtt gttatcttaa gaaatgctag
       61 acttaacgcg gctttttcat gatcattttc tgaaacgaac agttttgtca tttttaaaag
      121 ttgttttgaa acatttttgt aacataacta ttatatcgtt gacacactgc tatggtacaa
      181 tttttatatc gttaagaaag acttaaatat atttagatat ttattaaatt aagccagtcg
      241 gaggaatgaa gagtgaaaaa gagtttaata tcagcagtaa tggtatgttc aatgaccttg
      301 actgccgtag catcaccgat cgctgccgcg gcggacgatt ttgattctca gatacaacag
      361 caagatcaaa agattgcaga cttgaaaaat caacaagcag atgcacaatc acaaatcgat
      421 gccttagaaa gccaagtttc tgaaatcaac acacaagcac aagacctatt agcaaaacaa
      481 gatacattgc gtcaagaatc tgcacaatta gtaaaagaca ttgcagattt acaagaacgt
      541 attgaaaaac gtgaagatac aatccaaaaa caagcacgcg aagcacaagt aagcaacaca
      601 agctcaaact acatcgatgc tgtattgaat gctgattcat tagcagatgc tattggacgc
      661 gttcaagcaa tgacaacgat ggtaaaagca aacaacgact tgatggaaca acaaaaacaa
      721 gataaaaaag ctgtagaaga caaaaaagct gaaaacgatg ctaaactaaa agaattagct
      781 gaaaaccaag ctgctttgga atctcaaaaa ggcgatcttt tatctaaaca agctgatttg
      841 aacgtactga aaacttcatt agctgctgaa caagcaactg ctgaagacaa gaaagctgac
      901 ttgaaccgtc aaaaagcaga agctgaagca gagcaagctc gtattcgtga acaacaacgt
      961 ctagcagaac aagctcgtca acaagctgca caagaaaaag ctgaaaaaga agcacgcgaa
     1021 caagcagagg ctgaagcaca agctactcaa gcttcatcta ctgcacaaag ctcagcaaca
     1081 gaagaatcat cagctacaca atcttcaatg actgaagaat catcatcagc tacacaatct
     1141 tcagcaacag aagaatcaac aacacctgaa tcttcaacag aagaatcaac agcgcctgaa
     1201 tcttcagcaa cagaagaatc aacaacagtg cctgaatctt cagcaacaga agaatcaaaa
     1261 acagtgcctg aatcttcaac aacagaagaa tcaacaacac ctgcgcctac aacaccatca
     1321 acagatcaaa gtgttgatac aggaaacggc acaggaagta gtactccggc tccaacgcca
     1381 acaccaacac ctgaacaacc aaaacctgta acacctgctc cagctccttc tggaagtgtg
     1441 aatggtgcag caatcgtagc agaagcttac aaatatattg gtactccata tgtttggggc
     1501 ggtaaagatc caagtggctt tgactgctca gggttcacac gctatgttta catgcaagta
     1561 actggtcgtg acatcggtgg ttggacagtt cctcaagaat cagctggaac aaaaatttca
     1621 gtttctcaag caaaagctgg agacttgtta ttctgggggt cacaaggcgg aacttaccac
     1681 gtagcgattg ctttaggcgg aggacaatat atccatgctc ctcaaccagg cgaaagcgta
     1741 aaagttggtt cagttcaatg gtttgcccct gactttgctg tcagcatgta agaatgatta
     1801 atcaaaaaga gttagcccat tctggactaa ctcttttt
//
LOCUS       DAFFQY010000151         1701 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFFQY010000151 DAFFQY010000000
VERSION     DAFFQY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.160"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..255
                     /locus_tag="LRB02_002564"
     CDS             <1..255
                     /locus_tag="LRB02_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660718.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="LRB02_002565"
                     /pseudo
     CDS             258..411
                     /locus_tag="LRB02_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="LRB02_002566"
     CDS             434..1639
                     /locus_tag="LRB02_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL4660719.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DAFFQY010000152         1637 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFFQY010000152 DAFFQY010000000
VERSION     DAFFQY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.161"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(184..1503)
                     /locus_tag="LRB02_002567"
     CDS             complement(184..1503)
                     /locus_tag="LRB02_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL4660720.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          300 c          325 g          526 t
ORIGIN      
        1 aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga
       61 ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta
      121 aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt
      181 ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg
      241 atacacatga taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc
      301 agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc
      361 aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg
      421 gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc
      481 ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg
      541 agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc
      601 ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt
      661 ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga
      721 tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt
      781 ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa
      841 gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt
      901 tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt
      961 caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg tattataatt
     1021 cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc
     1081 cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc
     1141 atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag
     1201 ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga
     1261 atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgtc
     1321 atgagtaaaa tacgctcgtt tgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa
     1381 accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg ataattgacc
     1441 acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt gtaagctagt
     1501 catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc agaaaggggt
     1561 tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag
     1621 tttcacatgg ttttaat
//
LOCUS       DAFFQY010000153         1594 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFFQY010000153 DAFFQY010000000
VERSION     DAFFQY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.162"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1485
                     /locus_tag="LRB02_002568"
     CDS             <1..1485
                     /locus_tag="LRB02_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4660721.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          632 a          251 c          307 g          404 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atca
//
LOCUS       DAFFQY010000154         1515 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFFQY010000154 DAFFQY010000000
VERSION     DAFFQY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.163"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            287..694
                     /locus_tag="LRB02_002569"
     CDS             287..694
                     /locus_tag="LRB02_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660722.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LRB02_002570"
     CDS             708..1109
                     /locus_tag="LRB02_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660723.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LRB02_002571"
     CDS             1111..1482
                     /locus_tag="LRB02_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660724.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DAFFQY010000155         1500 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFFQY010000155 DAFFQY010000000
VERSION     DAFFQY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.164"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            8..514
                     /locus_tag="LRB02_002572"
     CDS             8..514
                     /locus_tag="LRB02_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660725.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            501..953
                     /locus_tag="LRB02_002573"
     CDS             501..953
                     /locus_tag="LRB02_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660726.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            946..1344
                     /locus_tag="LRB02_002574"
     CDS             946..1344
                     /locus_tag="LRB02_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660727.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          587 a          179 c          262 g          472 t
ORIGIN      
        1 tagtgagatg gacattaagt ggttggttca acaaaatgat agcaatttag aattggcgat
       61 taaatatctc gaagaaacta tctttgaaga tgaacatctt acagataatt ttttacaagt
      121 attaaaatat cttgaaattt actctgttaa aaaaaataaa ttaattggtg aaaatgattc
      181 tccaataaaa acacctattg agctaagctt aagaaatcgg atgggaatac tccaaagatc
      241 ggaaatagta aaagaacttt tttatcataa atttagttat gaaataagac tagatgatac
      301 ttatgagcat tatagaattg tttttttcgt atacaattct atagaagatg ctactgcgac
      361 tactgcatta acattcggtt ttacgaaaaa tggaacaatc aattcggata aaacccgtca
      421 agctgctaca gaaagtgatg atatatgtaa gaaagtatgc aatggggaag agaattactg
      481 gataggggag gaaaagttga atgaaatcta ttaatgattt attaaaaaaa catcctgatt
      541 tatataaaaa attaaattct gaacaaagaa acctgtccaa acaattactt aaaataagat
      601 taaaaaaaca aaaaaacgtg acagaattct caaaaatgtt aggaataagt gaaaaaaaat
      661 atcttgaatt tgagttcgga gatatgcata ttcctgttgc agagtacaat aaacttttga
      721 aacaagttga aaatttgtcc tataaagatt caatgaattt cactattaag gttgctggcg
      781 gccctattaa atatgaccgt attgaaaata aaccttatat tcggaataaa agttactcag
      841 tatttaaaag tagaaagaga aaagaagtat cgtcaaatat ggtctatgct agtgttaatt
      901 accaagatac gagccgctat attgtagaaa aagaggtata tgtacatgga taaaggaaat
      961 agtgttactt ttgcacttga aaatattagt ttgaataaaa ttcattttga attaactgat
     1021 aattttagtt atggagattt agagtttaat attgaacttg gatataaaga agacgaagca
     1081 gagtatctag aaaaaaaact tgtttcttta cgagctcaca ttgtaggggg gatggaagga
     1141 gcaccggtag atattttact cttttcagtt attcgtgttt ctacaaatga acagccatat
     1201 aaaaaattag tagaagaatc tatgggagag ctagctaaac ctatcctgaa taaagcttct
     1261 aatttgattt ctaatttata tagcgaagta ggagttatgc ctacagttat tgatttatat
     1321 cagctatata gtcaacaaaa ataaaataca agtaatagag ttatctattt aaatttgcaa
     1381 gacaaagacc tcaaccagtc atccttggtt gaggcttttt tgctctgtaa ttaactcgta
     1441 tagatcctgc aagatagttt gtttctgaat aaatgattga tatgaagtat ttagatgact
//
LOCUS       DAFFQY010000156         1456 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFFQY010000156 DAFFQY010000000
VERSION     DAFFQY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.165"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LRB02_002575"
     CDS             108..1040
                     /locus_tag="LRB02_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL4660728.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LRB02_002576"
     CDS             1112..>1456
                     /locus_tag="LRB02_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660729.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFFQY010000157         1445 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFFQY010000157 DAFFQY010000000
VERSION     DAFFQY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.166"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..226
                     /locus_tag="LRB02_002577"
     CDS             <1..226
                     /locus_tag="LRB02_002577"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBL4660730.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="LRB02_002578"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="LRB02_002578"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="LRB02_002579"
     CDS             533..1336
                     /locus_tag="LRB02_002579"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL4660731.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DAFFQY010000158         1409 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFFQY010000158 DAFFQY010000000
VERSION     DAFFQY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.167"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1359
                     /locus_tag="LRB02_002580"
     CDS             <1..1359
                     /locus_tag="LRB02_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL4660732.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAFFQY010000159         1368 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFFQY010000159 DAFFQY010000000
VERSION     DAFFQY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.169"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            82..>1368
                     /locus_tag="LRB02_002581"
     CDS             82..>1368
                     /locus_tag="LRB02_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL4660733.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAFFQY010000160         1355 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFFQY010000160 DAFFQY010000000
VERSION     DAFFQY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.170"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1355
                     /locus_tag="LRB02_002582"
     rRNA            <1..1355
                     /locus_tag="LRB02_002582"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAFFQY010000161         1282 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFFQY010000161 DAFFQY010000000
VERSION     DAFFQY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.172"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            81..1253
                     /locus_tag="LRB02_002583"
     CDS             81..1253
                     /locus_tag="LRB02_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBL4660734.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          478 a          235 c          227 g          342 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcgatc aaccaaaata ttcaaaagct tgcgcatcat
     1021 tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc cacaatcgac
     1081 taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga gaaaagatta
     1141 ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt cttatggacc
     1201 tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag tagaaatggt
     1261 aaaaacattg tatagcattt ta
//
LOCUS       DAFFQY010000162         1276 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFFQY010000162 DAFFQY010000000
VERSION     DAFFQY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.173"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            35..532
                     /locus_tag="LRB02_002584"
     CDS             35..532
                     /locus_tag="LRB02_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4660735.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="LRB02_002585"
     CDS             complement(569..1252)
                     /locus_tag="LRB02_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660736.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAFFQY010000163         1215 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFFQY010000163 DAFFQY010000000
VERSION     DAFFQY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.174"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            414..947
                     /locus_tag="LRB02_002586"
     CDS             414..947
                     /locus_tag="LRB02_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL4660737.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAFFQY010000164         1193 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFFQY010000164 DAFFQY010000000
VERSION     DAFFQY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.175"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LRB02_002587"
     CDS             <1..1076
                     /locus_tag="LRB02_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4660738.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFFQY010000165         1188 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFFQY010000165 DAFFQY010000000
VERSION     DAFFQY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.176"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LRB02_002588"
     CDS             141..398
                     /locus_tag="LRB02_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660739.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LRB02_002589"
     CDS             complement(630..1061)
                     /locus_tag="LRB02_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL4660740.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DAFFQY010000166         1156 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFFQY010000166 DAFFQY010000000
VERSION     DAFFQY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.177"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="LRB02_002590"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="LRB02_002590"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL4660741.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          294 a          259 c          180 g          423 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcactt
//
LOCUS       DAFFQY010000167         1091 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFFQY010000167 DAFFQY010000000
VERSION     DAFFQY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.178"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LRB02_002591"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LRB02_002591"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL4660742.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DAFFQY010000168         1063 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFFQY010000168 DAFFQY010000000
VERSION     DAFFQY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.179"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            700..>1063
                     /locus_tag="LRB02_002592"
     CDS             700..>1063
                     /locus_tag="LRB02_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4660743.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPL"
BASE COUNT          410 a          159 c          238 g          256 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc tag
//
LOCUS       DAFFQY010000169         1035 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFFQY010000169 DAFFQY010000000
VERSION     DAFFQY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.180"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..962)
                     /locus_tag="LRB02_002593"
     CDS             complement(<1..962)
                     /locus_tag="LRB02_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4660744.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT          287 a          179 c          145 g          424 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttt
//
LOCUS       DAFFQY010000170         1020 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFFQY010000170 DAFFQY010000000
VERSION     DAFFQY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.181"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            73..>1020
                     /locus_tag="LRB02_002594"
     CDS             73..>1020
                     /locus_tag="LRB02_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4660745.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLS"
BASE COUNT          353 a          175 c          189 g          303 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct
//
LOCUS       DAFFQY010000171         1006 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFFQY010000171 DAFFQY010000000
VERSION     DAFFQY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.182"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(63..626)
                     /locus_tag="LRB02_002595"
     CDS             complement(63..626)
                     /locus_tag="LRB02_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4660746.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          323 a          182 c          148 g          353 t
ORIGIN      
        1 ataataaaaa aatcactcta attctcccat aaatattata gattcctaaa gattaaaata
       61 gattattccc tattgtcttt taagtagcgg tagagtgttg ccttgctaat accagtcatt
      121 tccgtaattt cttttacgct ataaacctga cttttataca attttactgc tcgatcaaca
      181 tctttctggt tcactcgtgg acggccacct ttacgacctc tcgcacgtgc acttttcaaa
      241 ccttctttgg tacgttcaac aattaaatca cgttcgaact gactaaatgc ttggaagaca
      301 gtcatcatta agcgtccgtg aggcgtgctg gtatcgaagt tttctttcaa gctcaccagc
      361 ttaacatggt gttcttcaaa ataggtaaca aggtcaatca aatcctttgt actacgaccc
      421 aatcgagaaa aactttctac aattaccgta tctcccggac gaattttatc ttttaaacga
      481 tttagttggg gacgaaccgc tttggttccc gtcattttct cagttaggat ttcattacaa
      541 ttttgttcat tgagtaagtc taattgacga tttagttctt gtgaacgtgt actcactcgg
      601 gcataaccat aaatgtatcc actcatgctt ttcttccttt cgtactttaa ttctggcttg
      661 attgtatcat aaaagggagt tatagataca tagttaatgc aacaagttat gatacaaaat
      721 atgacctctt ttatttaata aaagaggtca tttcattttg tctcataatc gattgttttt
      781 gatacgagtg tgtatacgaa ataaacaaaa aaatcttaag taaatttcta gatacctaat
      841 ttaatcctaa ctagtgtatc ctaaagttaa taacgagttt atcacaatga aaattataaa
      901 atgctcccat attagatata acatgcaata tttgaattag aactacaaaa tttattcgtt
      961 ttataaagta aataatgttg agatactaag gaatagaata ttataa
//
LOCUS       DAFFQY010000172          994 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFFQY010000172 DAFFQY010000000
VERSION     DAFFQY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.183"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..>994)
                     /locus_tag="LRB02_002596"
     rRNA            complement(<1..>994)
                     /locus_tag="LRB02_002596"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAFFQY010000173          951 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFFQY010000173 DAFFQY010000000
VERSION     DAFFQY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.184"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(71..820)
                     /locus_tag="LRB02_002597"
     CDS             complement(71..820)
                     /locus_tag="LRB02_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL4660747.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          315 a          177 c          176 g          283 t
ORIGIN      
        1 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
       61 ctcagttttt ttatgcgtgt tgttgtgcgt tgtagtattc ttcttccatt ttttgcatac
      121 gttcacgttc tttttggtta taccaaggtg aaacggtaaa tgcaaggaca tcattcagca
      181 agtaaacaga gctgttcacg aacatcgcca aacttgcaga tccatgcgta aatgaaatat
      241 accaaaggac catttgagca agccctgaag ctaaccacca gtaatattgg ttgttgtaac
      301 gcaagaaact gatgatacca gcagtcaagc taatagaaaa agcaatggca tctacccacg
      361 gccgaggatc atctgtataa cgaccaatca ggaatcctga aacaagatag acaaggaaag
      421 tactgatgat tgcgataaac cagctttttc cagtgaattt tcggattttt gaagccatat
      481 tatgattcca ttctttactt aacaatacgg ggatatccaa tgtgatcata taagcaatct
      541 gttcaccgat acttaaatag ttctttgctg aaaacccaac gataataaaa caaatcgcag
      601 acaaaatacc taaaataccg ttgactgatt ttgccgcatt gatggataaa atacacaata
      661 caccaagcgt ggtaccgata aatgtaataa cggacagcca agtgatggga ctattgacaa
      721 gagtcatgac ctggcagcca agactgaaaa agaaaagata ataattttgt tgcggccagc
      781 cttttagctg atgagctaaa aactgaaaat atgacttcat gtatgaggac tccttaagac
      841 actaaatgta gtgtgttctg tatttactaa cacaatctat tgtatgcttt tcttgaaaaa
      901 aaactagtct ttcaattata aataatttgt gttatgataa ttttctgttt g
//
LOCUS       DAFFQY010000174          949 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFFQY010000174 DAFFQY010000000
VERSION     DAFFQY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.185"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            60..>949
                     /locus_tag="LRB02_002598"
     CDS             60..>949
                     /locus_tag="LRB02_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HBL4660748.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKW"
BASE COUNT          305 a          194 c          197 g          253 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa attctaaaga ctctagcgaa caaaggcgat ttgaatgaat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtacg gggaacttca tctccagatt ccgcgcgacc
      301 gcaacggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gataccttag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc
      481 tgactgaaga agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt
      541 tcatggatgc tacttatatt cctttaaaac ggcaaaccgt ctccaaagaa gcaatctata
      601 ttgccattgg tatacgagaa gacggcacta aagaagtact aagttatgcg attgccccga
      661 ctgaatcgac ctacgtttgg aatgagttgt tacaggacat ttactccaga ggggttcatg
      721 atgtcttact gtttattacc gatggcttaa aaggcatgaa agatacgatt caccagattt
      781 atcctaaagc taaataccaa cattgctgtg ttcacgtttc ccgtaatatt gctcataaag
      841 tacgtgtcaa agatcgaaaa gaaatctgtg atgattttaa ggctgtttat caagctagct
      901 caaaggaaga ggcaaatacc tttttaggga gtatgattga gaagtggca
//
LOCUS       DAFFQY010000175          901 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFFQY010000175 DAFFQY010000000
VERSION     DAFFQY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.187"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..563)
                     /locus_tag="LRB02_002599"
     CDS             complement(<1..563)
                     /locus_tag="LRB02_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL4660749.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
     gene            complement(574..780)
                     /locus_tag="LRB02_002600"
     CDS             complement(574..780)
                     /locus_tag="LRB02_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL4660750.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          247 a          201 c          145 g          308 t
ORIGIN      
        1 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
       61 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      121 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      181 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      241 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      301 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      361 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      421 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      481 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      541 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      601 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      661 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      721 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      781 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      841 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      901 c
//
LOCUS       DAFFQY010000176          850 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFFQY010000176 DAFFQY010000000
VERSION     DAFFQY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.189"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            453..536
                     /locus_tag="LRB02_002601"
     tRNA            453..536
                     /locus_tag="LRB02_002601"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:487..489,aa:Leu,seq:cag)
BASE COUNT          349 a          112 c          161 g          228 t
ORIGIN      
        1 ttttatttat tgtgtcatat cgtattaaga atcatcgaac aatacaaata tgaattacaa
       61 taaaaaaatt atttgaagtt cgttcattcg ttacttatga tctctagaag aaagaaaacg
      121 tgaattactt cagcgtctag aattccagaa ggatagtaat caacagaaga aataatcaat
      181 atccaaatga aagaagatag ctgaatcgag aaaaagagga aaaaagaaat gaagaaaaaa
      241 ataaaatagt tactaatccc agagtcgttc gtttaacatt gaaatcgatc atatggataa
      301 aacagatttt ttcaccaacc ctcaatagat gatggagttg ctcggatgaa ttatttgtgt
      361 agatggcata agggagggcg tacagaaatg aaaaagaaca aaaatatgtt cttgacaaaa
      421 aacacatgta atcctaaact aaaagatgtg atgcggctat ggcggaattg gcagacgcgc
      481 tagcttcagg cgctagtgag ggtgacctcg tggaagttcg agtcttctta gccgcataat
      541 ttgttttttt ataaagagaa ggtacacctg gagcgatgag aaagaaaata acatattatc
      601 taaaagaata taggaaaaat tttaaacaac ggaaccagat tcaaataaat caaaaaaaat
      661 ttgtgtgaag gtgggacagt ttaaaaccta atagattgat tcaaatagta gatttataga
      721 ttctgataca agaaatactg gcaaacgata gttaaggaat aaagactaaa cttaaactca
      781 aaaagaggtc atgcgaaaga ataaatcgca tgacctctta ctatattttg aatgtgttta
      841 aacgttcaaa
//
LOCUS       DAFFQY010000177          841 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFFQY010000177 DAFFQY010000000
VERSION     DAFFQY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.190"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..146)
                     /locus_tag="LRB02_002602"
     CDS             complement(<1..146)
                     /locus_tag="LRB02_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660751.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LRB02_002603"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LRB02_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL4660752.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="LRB02_002604"
     CDS             complement(591..785)
                     /locus_tag="LRB02_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660753.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          244 a          168 c          146 g          283 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 t
//
LOCUS       DAFFQY010000178          795 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFFQY010000178 DAFFQY010000000
VERSION     DAFFQY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.191"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            102..749
                     /locus_tag="LRB02_002605"
     CDS             102..749
                     /locus_tag="LRB02_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660754.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
BASE COUNT          261 a          118 c          171 g          245 t
ORIGIN      
        1 tgtgtgattt attttctttt ctaattcaac ctgtggtatg atgagagcat aagtatcatg
       61 tgaaatgata tgaagtatgt ggaagatgga cggggaagaa tatggaagca aaaatcactt
      121 tagaaccttt tgaacgtatc ttaagcggtt accgcaaagt agaagagtta gctgtgaacg
      181 taactgattg ttcaaaatta gcgcaaaaat acgcaagatt cggcgttgaa ggctatcgat
      241 taggaaacta tgtcggtaca gggtatctga accgttattt agagtgtatg gtggaccgcg
      301 caccaatgct gatttataga caaaattatc tgattccgct gctttttcgt cgttctgatt
      361 cagcctttcg gttatttgaa gaagagtatc ggatggaggc tttctttcta ctattagagt
      421 ggagcttgaa acatcgtcct gaaaaaattc ttatcgaaag aaatgagaag atagacacga
      481 aaaaaaataa agtgatcgat agtgcatacc tagcatttcg tgtatcagag attttggatt
      541 gtggcggcta tccaatcagc aatttccaat caattgacca attcattgaa tggaatcgaa
      601 tttaccgatt gatcgacaat ggcgggattg gccgtcatag caaggtattt gacccagaat
      661 atccagaaaa catggaagaa ctgaaaatga ttatttcgct tgtcaaatta aaatatcctg
      721 aaacagattt ggatttatat atagaataaa tgaccttaaa tgattgaatt actttttctg
      781 caattattat tgtaa
//
LOCUS       DAFFQY010000179          787 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFFQY010000179 DAFFQY010000000
VERSION     DAFFQY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.192"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            24..716
                     /locus_tag="LRB02_002606"
     CDS             24..716
                     /locus_tag="LRB02_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL4660755.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAFFQY010000180          772 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFFQY010000180 DAFFQY010000000
VERSION     DAFFQY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.193"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..204)
                     /locus_tag="LRB02_002607"
     CDS             complement(<1..204)
                     /locus_tag="LRB02_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660756.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
     gene            complement(201..425)
                     /locus_tag="LRB02_002608"
     CDS             complement(201..425)
                     /locus_tag="LRB02_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL4660757.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(422..715)
                     /locus_tag="LRB02_002609"
     CDS             complement(422..715)
                     /locus_tag="LRB02_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660758.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
BASE COUNT          223 a          158 c          122 g          269 t
ORIGIN      
        1 tttcatgttg gcggctacgt ttcttgctgg tgcatctatt ccagtttcgt gcaaaataat
       61 tttgttagga attgctaatc tggagtctcc ttgattcggc gctaaattat actcgtcatt
      121 aatagtataa gcaaacgtat taatgggtaa taaaaaaaga gccgttaaca ggctcatcgc
      181 agtaatagta attttctttt tcatttgttt cctccttctt cgctttcagc cgagaacatt
      241 ttgtaggttc gatttgatac acccaacaca ctccctaaaa acgcgccaaa accagtaatg
      301 atgacaacac agatatctgt gtactgccaa ttgagcgctt taccaactaa ccccacgaaa
      361 gtagctagtg cgggaataat taccagtgcg aaccatttta gtacttcgaa cgttttatta
      421 ttcattttct tctctcccta aataaagttt taatttgttg cgtgtgttct accaattttt
      481 ctgcatgtgt atctaatctt tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat
      541 ctgatttgct cgattctaaa tctttagtca gcaaatctaa attgtgactt acttttgaaa
      601 gagtctcagt aatcttcgag aaagatgcag taattggttt tattactaat aaaatcaaag
      661 aaacgatagc ggttattgat cctgctatcg ctccccattc ccctaaatta atcatgtgac
      721 aactccttga atcaaaataa aaagcacatc aattaagatg cgctctcttc tt
//
LOCUS       DAFFQY010000181          741 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFFQY010000181 DAFFQY010000000
VERSION     DAFFQY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.194"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>741
                     /locus_tag="LRB02_002610"
     rRNA            <1..>741
                     /locus_tag="LRB02_002610"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DAFFQY010000182          736 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFFQY010000182 DAFFQY010000000
VERSION     DAFFQY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.195"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            221..>736
                     /locus_tag="LRB02_002611"
     CDS             221..>736
                     /locus_tag="LRB02_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL4660759.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT          255 a          115 c          163 g          203 t
ORIGIN      
        1 cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt ttctaaaagt
       61 tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg tttgtgagta
      121 tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta agaacgcata
      181 ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga ttaacagagt
      241 aacctctatt cttattcagc tgcaatcaaa aaaaataatt cctgccaaag aaattgcaca
      301 gcgatttaat ataagcttaa ggacagttta cagagatatc cggacacttg aagaagccgg
      361 aataccaatt ggatctgagg ccggaaaagg atattttctt gtagagggct tcctacttcc
      421 gccggtaatg tttacagcgg cggaagtggg tgcattgatt acagcaggga aatttttaaa
      481 ttgccatgga gatgaatcat ttataaagga ttttgattca gctatgtata aaatcaaatc
      541 tattttgaag catggtgaaa aaaactatgc acaggagttg gagaacagta ttaacgtgta
      601 cagcacatct ggacagaaaa atacattggc ggataacgtt attgcagcaa ttcagactgc
      661 aatctgcaat aaaagggtaa tatcaattca atatcctgca tcaggagggc aagaaccgga
      721 aagcagaatg atagag
//
LOCUS       DAFFQY010000183          718 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFFQY010000183 DAFFQY010000000
VERSION     DAFFQY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.196"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..696
                     /locus_tag="LRB02_002612"
     CDS             <1..696
                     /locus_tag="LRB02_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4660760.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT          241 a          146 c          127 g          204 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc atatggcttt cgtagctttt ataatttcaa attgcgcatc
      661 atgattcgtc atggcaacgc attgattttc aactaaaaaa gatcaagagg aactgatc
//
LOCUS       DAFFQY010000184          703 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFFQY010000184 DAFFQY010000000
VERSION     DAFFQY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.197"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..616)
                     /locus_tag="LRB02_002613"
     CDS             complement(<1..616)
                     /locus_tag="LRB02_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL4660761.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQ"
BASE COUNT          202 a          147 c          120 g          234 t
ORIGIN      
        1 cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga atcaatgccg ataccaatat
       61 atacaccttt tgagacaata cgatgatttt cccttacttt catatacagg gcatcacaca
      121 tgaaaaatgg atattccgag ccttctaggc tccggtttct ccactcaaaa acaaatggat
      181 ccaactgttt catcagattt gaaacgaatg atttagaaac agtcgcacca tccgttagca
      241 gttcaactgt tttagttact ttgcgagtgg agacaccaga aatgaccatt tcaatcatgg
      301 cagcgatcaa agctttttcg cttcgctgat atctttcaaa gatagtagga ctaaacttac
      361 cgtctctggt tcgtggaaca tgaagttcta acgtaccaat tcgagttgta aacgaacgat
      421 cgtagtaacc atttctttgt gattgccgtt cttctgaacg ttcataggca tttgcctgaa
      481 tatattgatt tcgttgttct tccatcagct gattaaacat gatagtcata agtgtttttg
      541 cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat ttcttcgctt tcaaaagtaa
      601 aatgtacttg agtcattgat atcagtccta ttcgagtatg ttttctgcaa aaaacattgt
      661 actcctaact gagatcaatg gcttttttga tgcttcactt tta
//
LOCUS       DAFFQY010000185          640 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFFQY010000185 DAFFQY010000000
VERSION     DAFFQY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.200"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>640
                     /locus_tag="LRB02_002614"
     CDS             <1..>640
                     /locus_tag="LRB02_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL4660762.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQY"
BASE COUNT          277 a           93 c          104 g          166 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc
//
LOCUS       DAFFQY010000186          635 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFFQY010000186 DAFFQY010000000
VERSION     DAFFQY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.201"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..278
                     /locus_tag="LRB02_002615"
     CDS             <1..278
                     /locus_tag="LRB02_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660763.1"
                     /translation="YASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPF
                     RGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            355..>635
                     /locus_tag="LRB02_002616"
     CDS             355..>635
                     /locus_tag="LRB02_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL4660764.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT          208 a          114 c          152 g          161 t
ORIGIN      
        1 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
       61 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
      121 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
      181 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
      241 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
      301 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
      361 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
      421 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
      481 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
      541 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
      601 agtcgtcaga tactctacta cagcttctgt gtgcc
//
LOCUS       DAFFQY010000187          608 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFFQY010000187 DAFFQY010000000
VERSION     DAFFQY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.202"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(80..>608)
                     /locus_tag="LRB02_002617"
     CDS             complement(80..>608)
                     /locus_tag="LRB02_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL4660765.1"
                     /translation="VQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIF
                     DKLPKKVSSDVKDELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDA
                     IQILSFPENIRRRIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEW
                     LASPRKYLEFNSNNI"
BASE COUNT          179 a          134 c          112 g          183 t
ORIGIN      
        1 ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag aaagaagaga
       61 ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat tttcgtggag
      121 aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga gtgatggaat
      181 tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg ttcaaccgtt
      241 ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa
      301 tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac
      361 catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa
      421 agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa
      481 tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa
      541 cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc
      601 gttgaacg
//
LOCUS       DAFFQY010000188          588 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFFQY010000188 DAFFQY010000000
VERSION     DAFFQY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.203"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            26..412
                     /locus_tag="LRB02_002618"
     CDS             26..412
                     /locus_tag="LRB02_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4660766.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          222 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaaa
//
LOCUS       DAFFQY010000189          562 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFFQY010000189 DAFFQY010000000
VERSION     DAFFQY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.204"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(70..468)
                     /gene="spxA"
                     /locus_tag="LRB02_002619"
     CDS             complement(70..468)
                     /gene="spxA"
                     /locus_tag="LRB02_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL4660767.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          163 a          116 c           90 g          193 t
ORIGIN      
        1 ttctaagatt tctctttacc aacatcattt gacaaagagc cagtcttttc caagtttcgt
       61 attatccttc tataatccag ccatcaattg tgcttgacgt aattctaatt gtcgtacatc
      121 gcgcggtagg aaccgacgaa tttcatcttc attaaaacca acttgcagac gtttatcatc
      181 aatcatgatt ggacggcgaa gcaatccagg attgttttgc acaagttcca ataattcttg
      241 taacggcaga tcatctaaat ccatgttcag tttttggaat actttggaac gtgtcgagat
      301 gatttcttct gttccgtctt ctgtcatttg taaaatcgct tttaattcag aactgttcaa
      361 tggttctgaa aaaatattac gttcaacaaa tggaatctga tgttcttgca accacgcacg
      421 agcctttctg caggaagtac aacttgggga agtatataat gttaacaacg tgcaccactc
      481 cttttcgtga taagaatcac ttttttgtaa cagaagatca taataatctc ccatctgttt
      541 ctattataca taatcaactt tt
//
LOCUS       DAFFQY010000190          528 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFFQY010000190 DAFFQY010000000
VERSION     DAFFQY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.205"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LRB02_002620"
     tRNA            complement(5..90)
                     /locus_tag="LRB02_002620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LRB02_002621"
     tRNA            complement(107..178)
                     /locus_tag="LRB02_002621"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LRB02_002622"
     tRNA            complement(190..262)
                     /locus_tag="LRB02_002622"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LRB02_002623"
     tRNA            complement(280..361)
                     /locus_tag="LRB02_002623"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LRB02_002624"
     tRNA            complement(369..441)
                     /locus_tag="LRB02_002624"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LRB02_002625"
     tRNA            complement(445..517)
                     /locus_tag="LRB02_002625"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFFQY010000191          526 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFFQY010000191 DAFFQY010000000
VERSION     DAFFQY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.206"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(22..267)
                     /locus_tag="LRB02_002626"
     CDS             complement(22..267)
                     /locus_tag="LRB02_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL4660768.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..>526)
                     /locus_tag="LRB02_002627"
     CDS             complement(381..>526)
                     /locus_tag="LRB02_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBL4660769.1"
                     /translation="KIFHYYMQGVDFVDMAECLAMDHKQVMSAYGRVKLKIKKHLKFY
                     NEE"
BASE COUNT          170 a          102 c           75 g          179 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttag
//
LOCUS       DAFFQY010000192          509 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFFQY010000192 DAFFQY010000000
VERSION     DAFFQY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.207"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..132)
                     /locus_tag="LRB02_002628"
     CDS             complement(<1..132)
                     /locus_tag="LRB02_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4660770.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAFFQY010000193          488 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFFQY010000193 DAFFQY010000000
VERSION     DAFFQY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.208"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..414)
                     /locus_tag="LRB02_002629"
     CDS             complement(<1..414)
                     /locus_tag="LRB02_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660771.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DAFFQY010000194          478 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFFQY010000194 DAFFQY010000000
VERSION     DAFFQY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.209"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>478
                     /locus_tag="LRB02_002630"
     CDS             <1..>478
                     /locus_tag="LRB02_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4660772.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEEN"
BASE COUNT          202 a           56 c           67 g          153 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaa
//
LOCUS       DAFFQY010000195          475 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFFQY010000195 DAFFQY010000000
VERSION     DAFFQY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.210"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>475
                     /locus_tag="LRB02_002631"
     CDS             <1..>475
                     /locus_tag="LRB02_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL4660773.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLG"
BASE COUNT          167 a           94 c           78 g          136 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa tccatgaaaa gcttgccctt aagggcatta ctatatccgt
      421 tccgccacgt aaaaacatgg ataaatcaga aaaactagac cacagcttac ttgga
//
LOCUS       DAFFQY010000196          464 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFFQY010000196 DAFFQY010000000
VERSION     DAFFQY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.211"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            73..>464
                     /locus_tag="LRB02_002632"
     CDS             73..>464
                     /locus_tag="LRB02_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660774.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTP"
BASE COUNT          175 a           85 c           76 g          128 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctcc
//
LOCUS       DAFFQY010000197          460 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFFQY010000197 DAFFQY010000000
VERSION     DAFFQY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.212"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(20..91)
                     /locus_tag="LRB02_002633"
     tRNA            complement(20..91)
                     /locus_tag="LRB02_002633"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="LRB02_002634"
     tRNA            complement(113..200)
                     /locus_tag="LRB02_002634"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="LRB02_002635"
     tRNA            complement(225..298)
                     /locus_tag="LRB02_002635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="LRB02_002636"
     tRNA            complement(312..382)
                     /locus_tag="LRB02_002636"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="LRB02_002637"
     tRNA            complement(384..459)
                     /locus_tag="LRB02_002637"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAFFQY010000198          446 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFFQY010000198 DAFFQY010000000
VERSION     DAFFQY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.213"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(53..124)
                     /locus_tag="LRB02_002638"
     tRNA            complement(53..124)
                     /locus_tag="LRB02_002638"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Glu,seq:ttc)
     gene            complement(136..209)
                     /locus_tag="LRB02_002639"
     tRNA            complement(136..209)
                     /locus_tag="LRB02_002639"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Ile,seq:gat)
     gene            complement(222..292)
                     /locus_tag="LRB02_002640"
     tRNA            complement(222..292)
                     /locus_tag="LRB02_002640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Gly,seq:tcc)
     gene            complement(297..369)
                     /locus_tag="LRB02_002641"
     tRNA            complement(297..369)
                     /locus_tag="LRB02_002641"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
     gene            complement(373..445)
                     /locus_tag="LRB02_002642"
     tRNA            complement(373..445)
                     /locus_tag="LRB02_002642"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Asp,seq:gtc)
BASE COUNT          138 a          123 c          100 g           85 t
ORIGIN      
        1 taaaatcatt ctttcagata aaaagaaaaa gatactcaaa aagtatcttc catgacccgt
       61 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
      121 ggccaataaa ataaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg
      181 tgcgctctaa ccagctgagc tataggccca taaaaactaa aagcgggtga cgagaatcga
      241 actcgcgaca acagcttgga aggctgtggt tttaccacta aactacaccc gcataatggc
      301 gcgggacaga atcgaactgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
      361 taccgagcca aacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      421 atgttaaccc ctacaccacg gaacca
//
LOCUS       DAFFQY010000199          442 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFFQY010000199 DAFFQY010000000
VERSION     DAFFQY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.214"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            46..>442
                     /locus_tag="LRB02_002643"
     CDS             46..>442
                     /locus_tag="LRB02_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL4660775.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          138 a           83 c           86 g          135 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cg
//
LOCUS       DAFFQY010000200          440 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFFQY010000200 DAFFQY010000000
VERSION     DAFFQY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.215"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            11..>440
                     /locus_tag="LRB02_002644"
     CDS             11..>440
                     /locus_tag="LRB02_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4660776.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DAFFQY010000201          435 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFFQY010000201 DAFFQY010000000
VERSION     DAFFQY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.216"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..431
                     /locus_tag="LRB02_002645"
     CDS             <1..431
                     /locus_tag="LRB02_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660777.1"
                     /translation="LLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGY
                     QLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNG
                     PLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          155 a           89 c           75 g          116 t
ORIGIN      
        1 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
       61 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
      121 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
      181 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
      241 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
      301 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
      361 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
      421 taaccaaata aacag
//
LOCUS       DAFFQY010000202          432 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFFQY010000202 DAFFQY010000000
VERSION     DAFFQY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.217"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..417
                     /locus_tag="LRB02_002646"
     CDS             <1..417
                     /locus_tag="LRB02_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660778.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DAFFQY010000203          430 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFFQY010000203 DAFFQY010000000
VERSION     DAFFQY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.218"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>430
                     /locus_tag="LRB02_002647"
     CDS             <1..>430
                     /locus_tag="LRB02_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL4660779.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAFFQY010000204          407 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFFQY010000204 DAFFQY010000000
VERSION     DAFFQY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.219"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..384
                     /locus_tag="LRB02_002648"
     CDS             <1..384
                     /locus_tag="LRB02_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660780.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DAFFQY010000205          381 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFFQY010000205 DAFFQY010000000
VERSION     DAFFQY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.220"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..>381)
                     /locus_tag="LRB02_002649"
     CDS             complement(<1..>381)
                     /locus_tag="LRB02_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660781.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNS"
BASE COUNT          124 a           78 c           76 g          103 t
ORIGIN      
        1 gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa agttctgcca
       61 atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa aagctttttc
      121 accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc ttcacgagtc
      181 gctaatgaaa accgagaaaa atagcgttct aaataaggca agcgtcgatt ttcgacaata
      241 tccaataatg catggctttg tccgtccatc atgataaaac tcattgcact ggcaacactt
      301 cgaaccgatt tgaattcatc aaagcataag acctttggca aagtctgttt gaaaggattc
      361 aatgggcggt agaatcgctt c
//
LOCUS       DAFFQY010000206          381 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFFQY010000206 DAFFQY010000000
VERSION     DAFFQY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.221"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(21..94)
                     /locus_tag="LRB02_002650"
     tRNA            complement(21..94)
                     /locus_tag="LRB02_002650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="LRB02_002651"
     tRNA            complement(108..181)
                     /locus_tag="LRB02_002651"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="LRB02_002652"
     tRNA            complement(209..281)
                     /locus_tag="LRB02_002652"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="LRB02_002653"
     tRNA            complement(301..374)
                     /locus_tag="LRB02_002653"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAFFQY010000207          375 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFFQY010000207 DAFFQY010000000
VERSION     DAFFQY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.222"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(247..320)
                     /locus_tag="LRB02_002654"
     tRNA            complement(247..320)
                     /locus_tag="LRB02_002654"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(284..286),aa:Ile,seq:cat)
BASE COUNT          127 a           84 c           66 g           98 t
ORIGIN      
        1 tttctttttc aaaaaaagac gcttaccgct gcgaaacagt aagcgccatt gtcggctcta
       61 atgtttatct agccaatgac taaataatgc caaagttaaa ccaattaaca atggcaagag
      121 cacgtgctcc aaagtgaatt caaacaaatc atacacctcc tcccacgaaa atctctgtgg
      181 aagcgacgag ccgacatttt attataccat tattgaattt tattttttat caaatagatt
      241 aaattatgta ccctgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctctg
      301 accaactgag ctaagagtac aggttgttgc cacataaagc cataaacaat caaccagtag
      361 aaggtgtggc aacaa
//
LOCUS       DAFFQY010000208          372 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFFQY010000208 DAFFQY010000000
VERSION     DAFFQY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.223"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            82..>372
                     /locus_tag="LRB02_002655"
     CDS             82..>372
                     /locus_tag="LRB02_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL4660782.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          132 a           61 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cc
//
LOCUS       DAFFQY010000209          341 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFFQY010000209 DAFFQY010000000
VERSION     DAFFQY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.224"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>341
                     /locus_tag="LRB02_002656"
     CDS             <1..>341
                     /locus_tag="LRB02_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4660783.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAFFQY010000210          340 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFFQY010000210 DAFFQY010000000
VERSION     DAFFQY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.225"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(44..>340)
                     /locus_tag="LRB02_002657"
     CDS             complement(44..>340)
                     /locus_tag="LRB02_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660784.1"
                     /translation="EILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETL
                     AQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           95 a           86 c           61 g           98 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc
//
LOCUS       DAFFQY010000211          325 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFFQY010000211 DAFFQY010000000
VERSION     DAFFQY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.226"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
BASE COUNT          110 a           51 c           61 g          103 t
ORIGIN      
        1 gtatgtctta gtccctcaat aaacagtcga agagaaaata ggcaaaaatt gattactaaa
       61 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaacagat tcgaattaaa
      121 cgatttcgat tggcttatac cagtcctatc cgacgaaaga tatatcgttc ttaactctag
      181 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
      241 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
      301 aaaaacggtg gggttgttta gcgaa
//
LOCUS       DAFFQY010000212          322 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFFQY010000212 DAFFQY010000000
VERSION     DAFFQY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.227"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>322
                     /locus_tag="LRB02_002658"
     CDS             <1..>322
                     /locus_tag="LRB02_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660785.1"
                     /translation="DGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNY
                     RAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVS
                     KEKLA"
BASE COUNT          103 a           48 c           76 g           95 t
ORIGIN      
        1 gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg ggagctggta
       61 acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat aaagcattgt
      121 ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa aaatatggaa
      181 ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt tctcatttgt
      241 gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta tcgcaaatgg
      301 gagttagcaa agaaaagctt gc
//
LOCUS       DAFFQY010000213          309 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFFQY010000213 DAFFQY010000000
VERSION     DAFFQY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.228"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            51..>309
                     /locus_tag="LRB02_002659"
     CDS             51..>309
                     /locus_tag="LRB02_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL4660786.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DAFFQY010000214          307 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFFQY010000214 DAFFQY010000000
VERSION     DAFFQY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.229"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..298
                     /locus_tag="LRB02_002660"
     CDS             <1..298
                     /locus_tag="LRB02_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4660787.1"
                     /translation="KNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHML
                     RHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          135 a           51 c           35 g           86 t
ORIGIN      
        1 taagaataat aaagataaat acattttcat ctctaaaaat ggtaacccga tatctattca
       61 agcatataat caatctttaa gagcagtatc caaagaatta aacatagata aaaaagtttc
      121 ttctcacatg ttaaggcatt cccacatttc tctacttaca gaattaggaa tcccattaaa
      181 agccattatg gatcgtgtag gacatgaaga ttcaaaaaca acattaaaga tttatactca
      241 tacaactaaa aatatgcaaa atcaactagt agaaaaacta ggaaaaatag aaatttaaat
      301 tgcccct
//
LOCUS       DAFFQY010000215          291 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFFQY010000215 DAFFQY010000000
VERSION     DAFFQY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.230"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            76..>291
                     /locus_tag="LRB02_002661"
     CDS             76..>291
                     /locus_tag="LRB02_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4660788.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DAFFQY010000216          279 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFFQY010000216 DAFFQY010000000
VERSION     DAFFQY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.231"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>279
                     /locus_tag="LRB02_002662"
     CDS             <1..>279
                     /locus_tag="LRB02_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660789.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           86 a           68 c           50 g           75 t
ORIGIN      
        1 aagatcgcag actttacaaa ctaactcaat acttcctgcg ctttcctaga aaggcccgtt
       61 taaaggtcaa gtacctagtg atggacatga atgccagcta ttgtcagttg cttaaaacag
      121 tctttccaaa tgcggagatc gtaactgacc gctttcatat tgtccaacac atcaatcgca
      181 gcttcaatca attacgtgtc cagatcatga atcgttttcg aacctcacac tctgaaaatc
      241 agaagaagta ccgtcggctc aagcgatatt ggaaactct
//
LOCUS       DAFFQY010000217          273 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFFQY010000217 DAFFQY010000000
VERSION     DAFFQY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.232"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
BASE COUNT          106 a           47 c           44 g           76 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttg
//
LOCUS       DAFFQY010000218          268 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFFQY010000218 DAFFQY010000000
VERSION     DAFFQY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.233"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            87..>268
                     /locus_tag="LRB02_002663"
     CDS             87..>268
                     /locus_tag="LRB02_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4660790.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           86 t
ORIGIN      
        1 tttacctatg aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac
       61 tttttaacga ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa
      121 ctttggctgc cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata
      181 ccgttcaatc aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct
      241 tgatcaatgc cattgcagaa tcaaattc
//
LOCUS       DAFFQY010000219          263 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFFQY010000219 DAFFQY010000000
VERSION     DAFFQY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.234"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>263
                     /locus_tag="LRB02_002664"
     CDS             <1..>263
                     /locus_tag="LRB02_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660791.1"
                     /translation="LTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTG
                     KELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT           89 a           42 c           54 g           78 t
ORIGIN      
        1 ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga aaacgcaaac
       61 ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt cattatggac
      121 tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag accagatgta
      181 aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa tatgcgtaat
      241 tttcctagta agaaagtcta tgt
//
LOCUS       DAFFQY010000220          263 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFFQY010000220 DAFFQY010000000
VERSION     DAFFQY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.235"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>263
                     /locus_tag="LRB02_002665"
     CDS             <1..>263
                     /locus_tag="LRB02_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4660792.1"
                     /translation="LTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTG
                     KELARVRADGIYRSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT           91 a           40 c           52 g           80 t
ORIGIN      
        1 ctgacagatg gaacgaatta cgcccacatt gatcagtttg gagaaatcga aaatgcaaat
       61 ttgcatgtag ctggatggca cattgctaac tataaatacg agtatatctt cattatggat
      121 tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag atcagatgta
      181 aatcaagctt ataatacttc tggaaatgtt ggctatcatg tatcttttaa catgcgtaac
      241 ttccctaata agaaagtcta tgt
//
LOCUS       DAFFQY010000221          251 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFFQY010000221 DAFFQY010000000
VERSION     DAFFQY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.236"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..159
                     /locus_tag="LRB02_002666"
     CDS             <1..159
                     /locus_tag="LRB02_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBL4660793.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT          105 a           28 c           37 g           81 t
ORIGIN      
        1 attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg gggctgttat
       61 cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat
      121 gaatatgcga attataaatt atctattgtt atagattaat aataaaacta gcagatggta
      181 aaaatactaa tatgcgagac acacattcaa ccataactat ataacttatc aatttaaaac
      241 taaactgatg g
//
LOCUS       DAFFQY010000222          250 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFFQY010000222 DAFFQY010000000
VERSION     DAFFQY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.237"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>250
                     /locus_tag="LRB02_002667"
     CDS             <1..>250
                     /locus_tag="LRB02_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660794.1"
                     /translation="SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF
                     QLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPC"
BASE COUNT           70 a           57 c           45 g           78 t
ORIGIN      
        1 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca
       61 tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat tcatgacagt
      121 caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat caattgtgtt
      181 gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca tttgaacctt
      241 cgaccttgtt
//
LOCUS       DAFFQY010000223          236 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFFQY010000223 DAFFQY010000000
VERSION     DAFFQY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.238"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            26..>236
                     /locus_tag="LRB02_002668"
     CDS             26..>236
                     /locus_tag="LRB02_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4660795.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRL"
BASE COUNT           86 a           48 c           39 g           63 t
ORIGIN      
        1 aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag aaacattaga
       61 tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa aatacaaagg
      121 gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat gcatccactg
      181 taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc ggttgt
//
LOCUS       DAFFQY010000224          231 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFFQY010000224 DAFFQY010000000
VERSION     DAFFQY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.239"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..>231)
                     /locus_tag="LRB02_002669"
     CDS             complement(<1..>231)
                     /locus_tag="LRB02_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBL4660796.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEK"
BASE COUNT           60 a           48 c           37 g           86 t
ORIGIN      
        1 tccttttcta agtccaaagt aaaagggcgt tttccagcct ctattcgttg acgattgatc
       61 tcatcttcta attcatcctg ataggctttc gcttcatcat gggtcatttc tttcgtaaac
      121 ttatgtttat tggcgttagc tttgatgtgg gtagaatcca gatacaaatt atcctctgtt
      181 acaaaacctg ctttaactgc ttgttctaaa atataggcaa aaatatcctc a
//
LOCUS       DAFFQY010000225          228 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFFQY010000225 DAFFQY010000000
VERSION     DAFFQY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.240"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(3..>228)
                     /locus_tag="LRB02_002670"
     CDS             complement(3..>228)
                     /locus_tag="LRB02_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660797.1"
                     /translation="IIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKL
                     NEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT           75 a           38 c           23 g           92 t
ORIGIN      
        1 aactatttaa tggactgttt ccgatattct actggagata gtccatttaa ctttagtttt
       61 atccttttgt tattatacca cctaatatac tcatttaatt tactttgaaa tatctcaatt
      121 gatcggaatt tttctcgata aaaaaactct gatttaagca caccaaaaaa attttctatt
      181 acagaattat ctaagcaatt tccttttcta gacatgcttt gaataatg
//
LOCUS       DAFFQY010000226          227 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFFQY010000226 DAFFQY010000000
VERSION     DAFFQY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.241"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(<1..>227)
                     /locus_tag="LRB02_002671"
     rRNA            complement(<1..>227)
                     /locus_tag="LRB02_002671"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAFFQY010000227          227 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFFQY010000227 DAFFQY010000000
VERSION     DAFFQY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.242"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            1..227
                     /locus_tag="LRB02_002672"
                     /pseudo
     CDS             1..227
                     /locus_tag="LRB02_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAFFQY010000228          224 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFFQY010000228 DAFFQY010000000
VERSION     DAFFQY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.243"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>224
                     /locus_tag="LRB02_002673"
     CDS             <1..>224
                     /locus_tag="LRB02_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL4660798.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAFFQY010000229          222 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFFQY010000229 DAFFQY010000000
VERSION     DAFFQY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.244"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>222
                     /locus_tag="LRB02_002674"
     CDS             <1..>222
                     /locus_tag="LRB02_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4660799.1"
                     /translation="RKAYGTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFK
                     GQRTACNEAKTANVLDRTFTQEQPLEAIVT"
BASE COUNT           71 a           50 c           54 g           47 t
ORIGIN      
        1 cgaaaggctt acggtacccg aaaattgaag aagtgtttag caaagcgtgg gcttcaactc
       61 agtcggcgcc gaatcggtcg aatcatgaaa cgccgcggat tgacatctac ctatacgatc
      121 gctcatttta aagggcaacg aacagcttgt aatgaagcga aaacagcgaa tgtattagat
      181 cggaccttta cacaagaaca gccattggaa gccatcgtta cg
//
LOCUS       DAFFQY010000230          218 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFFQY010000230 DAFFQY010000000
VERSION     DAFFQY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.245"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>218
                     /locus_tag="LRB02_002675"
     CDS             <1..>218
                     /locus_tag="LRB02_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4660800.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT           66 a           39 c           44 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaatt
//
LOCUS       DAFFQY010000231          213 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFFQY010000231 DAFFQY010000000
VERSION     DAFFQY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.246"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>213
                     /locus_tag="LRB02_002676"
     CDS             <1..>213
                     /locus_tag="LRB02_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL4660801.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRP"
BASE COUNT           74 a           42 c           41 g           56 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tcc
//
LOCUS       DAFFQY010000232          209 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFFQY010000232 DAFFQY010000000
VERSION     DAFFQY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.247"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>209
                     /locus_tag="LRB02_002677"
     CDS             <1..>209
                     /locus_tag="LRB02_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4660802.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DAFFQY010000233          208 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFFQY010000233 DAFFQY010000000
VERSION     DAFFQY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.248"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
BASE COUNT           61 a           37 c           46 g           64 t
ORIGIN      
        1 ttttaaattt aattgtctga tatgcttgtg atgaattgat tttcgttctg cacttttgga
       61 cgataaattg tgataattga aaatacaaaa aaaagaaagg cccctgtaag gctcgggaga
      121 ctggtaatct ccgtcagtgt cctacaggaa agcattaaga tgacgcctta atgcgtgccc
      181 ttcagtgtta cggtattacc cgcaacgg
//
LOCUS       DAFFQY010000234          207 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFFQY010000234 DAFFQY010000000
VERSION     DAFFQY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.denovo.249"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..>207
                     /locus_tag="LRB02_002678"
     CDS             <1..>207
                     /locus_tag="LRB02_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4660803.1"
                     /translation="AVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDID
                     IYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           74 a           37 c           36 g           60 t
ORIGIN      
        1 ggcagttgat attgaaaatt cgattaatga atggcttcaa tccgtaccca aaaatctttt
       61 caaatcaatt acctttgatt gtggaaaaga attctctaat tggaaaagta ttagtaatac
      121 gaatgatatt gatatttatt tcgcagaccc aggaacgcct tcccaacggg gattaaatga
      181 acattcaaac ggactcctcc gaaaaga
//
LOCUS       DAFFQY010000235         3822 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.124, whole genome shotgun sequence.
ACCESSION   DAFFQY010000235 DAFFQY010000000
VERSION     DAFFQY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.124"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            332..796
                     /locus_tag="LRB02_002679"
     CDS             332..796
                     /locus_tag="LRB02_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4660804.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            780..1409
                     /locus_tag="LRB02_002680"
     CDS             780..1409
                     /locus_tag="LRB02_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4660805.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1763..3241
                     /gene="msr(C)"
                     /locus_tag="LRB02_002681"
     CDS             1763..3241
                     /gene="msr(C)"
                     /locus_tag="LRB02_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL4660806.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3288..3734
                     /locus_tag="LRB02_002682"
     CDS             3288..3734
                     /locus_tag="LRB02_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL4660807.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         1285 a          622 c          792 g         1123 t
ORIGIN      
        1 cttttttatt actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca
       61 aaaggcgatt tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc
      121 tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt
      181 ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta
      241 ttacacagct gattctttga cttttttctt tttttattct attattgaca agtcaatcgt
      301 tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt
      361 atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat
      421 aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag
      481 cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt
      541 ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt
      601 agcgctaaca gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac
      661 aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt
      721 agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa
      781 tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg
      841 atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg
      901 caccttcttc tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa
      961 aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa
     1021 tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag
     1081 cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc
     1141 cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct
     1201 tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg
     1261 gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc
     1321 cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag
     1381 caccagaaaa gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg
     1441 ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc
     1501 gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa
     1561 acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag
     1621 ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat
     1681 gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac
     1741 atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt
     1801 cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg
     1861 tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg
     1921 tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca
     1981 attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt
     2041 aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt
     2101 gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct
     2161 agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt
     2221 ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa
     2281 agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt
     2341 ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga
     2401 aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa
     2461 gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca
     2521 aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac
     2581 aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca
     2641 cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact
     2701 ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg
     2761 ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc
     2821 tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc
     2881 catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct
     2941 gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg
     3001 agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt
     3061 agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca
     3121 aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc
     3181 tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta
     3241 aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata
     3301 aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg
     3361 cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc
     3421 ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa
     3481 ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat
     3541 gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag
     3601 gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg
     3661 ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc
     3721 aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat
     3781 agtctcttga atgcagttct agaaaaatag tgatccgaac ga
//
LOCUS       DAFFQY010000236         2790 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.141, whole genome shotgun sequence.
ACCESSION   DAFFQY010000236 DAFFQY010000000
VERSION     DAFFQY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.141"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            104..1072
                     /locus_tag="LRB02_002683"
     CDS             104..1072
                     /locus_tag="LRB02_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL4660808.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1065..2096
                     /gene="vanA"
                     /locus_tag="LRB02_002684"
     CDS             1065..2096
                     /gene="vanA"
                     /locus_tag="LRB02_002684"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL4660809.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2102..2710
                     /locus_tag="LRB02_002685"
     CDS             2102..2710
                     /locus_tag="LRB02_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL4660810.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          822 a          537 c          687 g          744 t
ORIGIN      
        1 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
       61 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
      121 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
      181 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
      241 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
      301 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
      361 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
      421 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
      481 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
      541 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
      601 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
      661 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
      721 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
      781 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
      841 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
      901 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
      961 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     1021 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     1081 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     1141 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     1201 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     1261 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     1321 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     1381 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     1441 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     1501 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     1561 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     1621 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     1681 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     1741 tagctgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     1801 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     1861 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     1921 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     1981 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     2041 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     2101 catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa
     2161 atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat
     2221 tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca
     2281 agggtacgga ttgcttctat gggacggtta ccgtcctaat cgtgctgtaa actgttttat
     2341 gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga
     2401 ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag
     2461 tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg
     2521 atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc
     2581 gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct
     2641 cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt ttgatttccc
     2701 cgttaaataa actggttctg ttgcaaagtt ttaaataaag aataaaatct cttacggtat
     2761 ctatgattta agctgggatt cccaataata
//
LOCUS       DAFFQY010000237         2741 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.144, whole genome shotgun sequence.
ACCESSION   DAFFQY010000237 DAFFQY010000000
VERSION     DAFFQY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.144"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            72..647
                     /locus_tag="LRB02_002686"
     CDS             72..647
                     /locus_tag="LRB02_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4660811.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            861..1556
                     /gene="vanR"
                     /locus_tag="LRB02_002687"
     CDS             861..1556
                     /gene="vanR"
                     /locus_tag="LRB02_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL4660812.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1534..2688
                     /locus_tag="LRB02_002688"
     CDS             1534..2688
                     /locus_tag="LRB02_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL4660813.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          949 a          480 c          580 g          732 t
ORIGIN      
        1 aaatgtagta gactgataaa atcatagtta agagcgtctc ataagacttg tctcaaaaat
       61 gaggtgatat tttgcggaaa atcggttata ttcgtgtcag ttcgactaac cagaatcctt
      121 caagacaatt tcagcagttg aacgagatcg gaatggatat tatatatgaa gagaaagttt
      181 caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt agacgattta caggaagatg
      241 acatcattta tgttacagac ttaactcgaa tcactcgtag tacacaagat ctatttgaat
      301 taatcgataa catacgagat aaaaaggcaa gtttaaaatc actaaaagat acatggcttg
      361 atttatcaga agataatcca tacagccaat tcttaattac tgtaatggct ggtgttaacc
      421 aattagagcg agatcttatt cggatgagac aacgtgaagg gattgaattg gctaagaaag
      481 aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa tcacgcagga atgaattatg
      541 cggtaaagct atataaagaa ggaaatatga ctgtaaatca aatttgtgaa attactaatg
      601 tatctagggc ttcattatac aggaaattat cagaagtgaa taattagcca ttctgtattc
      661 cgctaatggg caatattttt aaagaagaaa aggaaactat aaaatattaa cagcctccta
      721 gcgatgccga aaagcccttt gataaaaaaa gaatcatcat cttaagaaat tcttagtcat
      781 ttattatgta aatgcttata aattcggccc tataatctga taaattatta agggcaaact
      841 tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa
      901 ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata
      961 ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca
     1021 tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct
     1081 atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa
     1141 tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa
     1201 aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta
     1261 tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc
     1321 agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga
     1381 atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga
     1441 gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg
     1501 ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa
     1561 aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta
     1621 gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc
     1681 ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa
     1741 tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt
     1801 attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc
     1861 attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt
     1921 atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag
     1981 ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc
     2041 cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat
     2101 caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc
     2161 gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac
     2221 atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca
     2281 catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat
     2341 aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac
     2401 agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac
     2461 actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac
     2521 aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt
     2581 attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg
     2641 gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga gatgtatata
     2701 attttggctc tttgtcaaat gatgttggta aagagaaatc t
//
LOCUS       DAFFQY010000238         2524 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.148, whole genome shotgun sequence.
ACCESSION   DAFFQY010000238 DAFFQY010000000
VERSION     DAFFQY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.148"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..363
                     /locus_tag="LRB02_002689"
     CDS             <1..363
                     /locus_tag="LRB02_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL4660814.1"
                     /translation="NFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFAC
                     NNAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIV
                     SEINNFLNEESSDERKQI"
     gene            344..1078
                     /locus_tag="LRB02_002690"
     CDS             344..1078
                     /locus_tag="LRB02_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4660815.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1111..2023
                     /gene="ant(6)"
                     /locus_tag="LRB02_002691"
                     /pseudo
     CDS             1111..2023
                     /gene="ant(6)"
                     /locus_tag="LRB02_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            2020..>2524
                     /gene="sat4"
                     /locus_tag="LRB02_002692"
     CDS             2020..>2524
                     /gene="sat4"
                     /locus_tag="LRB02_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL4660816.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEN"
BASE COUNT          887 a          361 c          556 g          720 t
ORIGIN      
        1 aactttttta tgtttgaagc agggttctct ttaatgtttg taaaagcaaa ttcgggaaca
       61 gacgataaat attatattgc gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg
      121 ttatttgcat gtaataatgc ttattgtatc aacgaaaaga aagctataaa actgcttgaa
      181 acttttgaac ataaacctga aaaatatacc gagaaggtaa atcatatttt tgaagtactc
      241 ggtatctcac tttttgaatg ctacgacatg accgagaagc tttataatga agtgaatgaa
      301 attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata
      361 tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc
      421 tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt
      481 gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc
      541 ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca
      601 ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag
      661 ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat
      721 aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc
      781 tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca
      841 tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa
      901 ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt
      961 tataataaat cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat
     1021 ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata
     1081 atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta
     1141 gtactttctt tagcagaaca ggatgaacga acgtattcga attgtgaccc ttgaggggtc
     1201 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     1261 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     1321 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     1381 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     1441 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     1501 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     1561 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     1621 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     1681 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     1741 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     1801 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     1861 ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa
     1921 tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga
     1981 tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca
     2041 gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata
     2101 ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta
     2161 aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt
     2221 gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt
     2281 aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat
     2341 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     2401 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     2461 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     2521 aaca
//
LOCUS       DAFFQY010000239         2465 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.150, whole genome shotgun sequence.
ACCESSION   DAFFQY010000239 DAFFQY010000000
VERSION     DAFFQY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.150"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..713
                     /locus_tag="LRB02_002693"
     CDS             <1..713
                     /locus_tag="LRB02_002693"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4660817.1"
                     /translation="GCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRKNVLVSKV
                     KLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDI
                     PGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGK
                     VPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLE
                     KTKILGQTLSRFFTILPK"
     gene            971..>2465
                     /gene="eat(A)"
                     /locus_tag="LRB02_002694"
     CDS             971..>2465
                     /gene="eat(A)"
                     /locus_tag="LRB02_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL4660818.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLE"
BASE COUNT          857 a          425 c          545 g          638 t
ORIGIN      
        1 gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct ttagaagggt
       61 acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga aaaaacgttc
      121 ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat acaggtcaac
      181 aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg ctagctttct
      241 tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag gtggatttag
      301 atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt ttgttgggag
      361 cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa aaaaagatcg
      421 aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg atccacggaa
      481 aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
      541 gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt gtcttaagta
      601 ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca ttagaaaaaa
      661 caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag tagttgacaa
      721 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
      781 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
      841 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
      901 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
      961 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
     1021 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
     1081 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
     1141 tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga caaaagataa
     1201 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
     1261 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
     1321 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
     1381 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
     1441 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
     1501 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
     1561 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
     1621 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
     1681 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     1741 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     1801 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     1861 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     1921 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     1981 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     2041 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     2101 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     2161 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     2221 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     2281 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     2341 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     2401 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     2461 tgaga
//
LOCUS       DAFFQY010000240         2442 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.151, whole genome shotgun sequence.
ACCESSION   DAFFQY010000240 DAFFQY010000000
VERSION     DAFFQY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.151"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            77..988
                     /locus_tag="LRB02_002695"
     CDS             77..988
                     /locus_tag="LRB02_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL4660819.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1141..1626
                     /gene="vanZ"
                     /locus_tag="LRB02_002696"
     CDS             1141..1626
                     /gene="vanZ"
                     /locus_tag="LRB02_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL4660820.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1913..2242)
                     /locus_tag="LRB02_002697"
     CDS             complement(1913..2242)
                     /locus_tag="LRB02_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4660821.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT          848 a          361 c          428 g          805 t
ORIGIN      
        1 gagattgtgc cgtttaaggg gaagattcta gaaatatttc atacttccaa ctatatagtt
       61 aaggaggaga ctgaaaatga agaagttgtt ttttttattg ttattgttat tcttaatata
      121 cttaggttat gactacgtta atgaagcact gttttctcag gaaaaagtcg aatttcaaaa
      181 ttatgatcaa aatcccaaag aacatttaga aaatagtggg acttctgaaa atacccaaga
      241 gaaaacaatt acagaagaac aggtttatca aggaaatctg ctattaatca atagtaaata
      301 tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac atgacgaatt
      361 aataaatgga tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa tagcacaaaa
      421 attttcagag atggtcaatg atgctgtaaa gggtggcgtt agtcatttta ttattaatag
      481 tggctatcga gactttgatg agcaaagtgt gctttaccaa gaaatggggg ctgagtatgc
      541 cttaccagca ggttatagtg agcataattc aggtttatca ctagatgtag gatcaagctt
      601 gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt ggaaatacgg
      661 gttcatttta cgttatccag aggacaaaac agagttaaca ggaattcaat atgaaccatg
      721 gcatattcgc tatgttggtt taccacatag tgcgattatg aaagaaaaga atttcgttct
      781 cgaggaatat atggattacc taaaagaaga aaaaaccatt tctgttagtg taaatgggga
      841 aaaatatgag atcttttatt atcctgttac taaaaatacc accattcatg tgccgactaa
      901 tcttcgttat gagatatcag gaaacaatat agacggtgta attgtgacag tgtttcccgg
      961 atcaacacat actaattcaa ggaggtaagg atggcggaat gaaaccaacg aaattaatga
     1021 acagcattat tgtactagca cttttggggt aacgttagct ttttaattta aaacccacgt
     1081 taactaggac attgctatac taatgataca acttaaacaa aagaattaga ggaaattata
     1141 ttgggaaaaa tattatctag aggattgcta gctttatatt tagtgacact aatctggtta
     1201 gtgttattca aattacaata caatatttta tcagtattta attatcatca aagaagtctt
     1261 aacttgactc catttactgc tactgggaat ttcagagaga tgatagataa tgttataatc
     1321 tttattccat ttggcttgct tttgaatgtc aattttaaag aaatcggatt tttacctaag
     1381 tttgcttttg tactggtttt aagtcttact tttgaaataa ttcaatttat cttcgctatt
     1441 ggagcgacag acataacaga tgtaattaca aatactgttg gaggctttct tggactgaaa
     1501 ttatatggtt taagcaataa gcatatgaat caaaaaaaat tagacagagt tattattttt
     1561 gtaggtatac ttttgctcgt attattgctc gtttaccgta cccatttaag aataaattac
     1621 gtgtaagatg tctaaatcaa gcaatctgat ctttcataca cataaagata ttgaatgaat
     1681 tggattagat ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa
     1741 accggtgata aagtaaaaag cttacctaac actatagtaa caaagaaagc ccaattatca
     1801 attttagtgc tgaggaattg gtctctttaa taaatttcct taacgttgta aatccgcatt
     1861 ttcctgacgg tacccccaaa ctaagatttc atagatagat tttctgataa ctttaaaaaa
     1921 cacgaatagc ggaaaggatg tcttcattcg ctaaagagta ataaactact tttccatctt
     1981 gacgtgattt tgccattcca tgttttttta aaaaacgtaa atggtgtgac gttgtagcga
     2041 cactagcagt aagtatttct gctaaatcac agacgcacat ttccttatag gtttctaaag
     2101 catagaaaat tttaattcta gaaaaatcgc tgaaacattt ccctaacaca agtaagtttg
     2161 aaaaatcttt ttgatccaat ttatttttta ttccatttac tttttcttta tgtacgacag
     2221 tcacttggca aacttttttc attcatctca cctcacattc aaacgattat ttgaatgaag
     2281 tatacctatt tgatcactct tttgtcaatc taccttaatt ccaatacact attataagca
     2341 atttcatgta ttgaagtaaa cttacttgca agtgcgtttt tgattccaag ttaaaaacat
     2401 attattattc tacataattt cctaatcaaa taaaactcct tt
//
LOCUS       DAFFQY010000241         2419 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.152, whole genome shotgun sequence.
ACCESSION   DAFFQY010000241 DAFFQY010000000
VERSION     DAFFQY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.152"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            complement(150..1253)
                     /locus_tag="LRB02_002698"
     CDS             complement(150..1253)
                     /locus_tag="LRB02_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4660822.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1806..2354
                     /gene="aac(6')"
                     /locus_tag="LRB02_002699"
     CDS             1806..2354
                     /gene="aac(6')"
                     /locus_tag="LRB02_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL4660823.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          777 a          425 c          468 g          749 t
ORIGIN      
        1 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
       61 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
      121 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
      181 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
      241 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
      301 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
      361 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
      421 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
      481 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
      541 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
      601 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
      661 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
      721 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
      781 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
      841 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
      901 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
      961 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
     1021 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
     1081 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
     1141 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
     1201 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
     1261 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
     1321 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
     1381 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
     1441 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
     1501 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
     1561 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
     1621 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
     1681 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
     1741 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
     1801 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
     1861 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
     1921 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
     1981 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
     2041 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
     2101 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
     2161 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
     2221 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
     2281 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
     2341 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
     2401 gaatttacga aaattactt
//
LOCUS       DAFFQY010000242         2103 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.155, whole genome shotgun sequence.
ACCESSION   DAFFQY010000242 DAFFQY010000000
VERSION     DAFFQY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.155"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            53..1972
                     /gene="tet(M)"
                     /locus_tag="LRB02_002700"
     CDS             53..1972
                     /gene="tet(M)"
                     /locus_tag="LRB02_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL4660824.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          721 a          320 c          437 g          625 t
ORIGIN      
        1 ttatcactgg gatttttatg cccttttggg cttttgaatg gaggaaaatc acatgaaaat
       61 tattaatatt ggagttttag ctcatgttga tgcgggaaaa actaccttaa cagaaagctt
      121 attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac
      181 ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt
      241 tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc
      301 agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga
      361 tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac
      421 aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga
      481 tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa
      541 tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga
      601 tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga
      661 ggaaagcata agatttcaga attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag
      721 caacataggg attgagcagc ttatagaagt gataacgaat aaattttatt catcaacata
      781 cagaaagaag tctgaacttt gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca
      841 acgtcttgca tatgtacgcc tttatggcgg aatcctgcat ttgcgggatt cggttagaat
      901 atcggaaaag gaaaaaataa aaattacaga aatgtatact tcaataaatg gtgaattatg
      961 taaaattgat aaggcttatt ccggggaaat tgttattttg caaaatgagt ttttgaagct
     1021 aaatagtgtt cttggagata caaagctatt gccacagaga gagagaattg aaaatccgct
     1081 ccctctgctg caaacaactg ttgaaccgag caaacctcaa caaagggaaa tgttacttga
     1141 tgcactttta gaaatctccg acagtgaccc gcttctacaa tattatgtgg attctacgac
     1201 acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt
     1261 gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag
     1321 accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc
     1381 ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc
     1441 ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta
     1501 tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct gttttaagta
     1561 tggcttatac tatagccctg ttagtacccc agcagatttt cggatgcttg ctcctattgt
     1621 attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa
     1681 aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta aatattgtgc
     1741 gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc
     1801 tcggtgtatt caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg
     1861 tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc agccccgtcg
     1921 tccaaatagt cggatagata aagtacgata tatgttcaat aaaataactt agtgtatttt
     1981 atgttgttat ataaatatgg tttcttgtta aataagatga aatatttttt aataaagatt
     2041 tgaattaaag tgtaaaggag gaattaatta tgaagtgtaa tgaatgtaac agggttaaat
     2101 aca
//
LOCUS       DAFFQY010000243         1920 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.158, whole genome shotgun sequence.
ACCESSION   DAFFQY010000243 DAFFQY010000000
VERSION     DAFFQY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.158"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..363
                     /locus_tag="LRB02_002701"
     CDS             <1..363
                     /locus_tag="LRB02_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL4660825.1"
                     /translation="NFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFAC
                     NNAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIV
                     SEINNFLNEESSDERKQI"
     gene            344..1078
                     /locus_tag="LRB02_002702"
     CDS             344..1078
                     /locus_tag="LRB02_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4660826.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1111..1920
                     /gene="ant(6)"
                     /locus_tag="LRB02_002703"
                     /pseudo
     CDS             1111..1920
                     /gene="ant(6)"
                     /locus_tag="LRB02_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
BASE COUNT          658 a          265 c          421 g          576 t
ORIGIN      
        1 aactttttta tgtttgaagc agggttctct ttaatgtttg taaaagcaaa ttcgggaaca
       61 gacgataaat attatattgc gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg
      121 ttatttgcat gtaataatgc ttattgtatc aacgaaaaga aagctataaa actgcttgaa
      181 acttttgaac ataaacctga aaaatatacc gagaaggtaa atcatatttt tgaagtactc
      241 ggtatctcac tttttgaatg ctacgacatg accgagaagc tttataatga agtgaatgaa
      301 attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata
      361 tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc
      421 tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt
      481 gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc
      541 ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca
      601 ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag
      661 ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat
      721 aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc
      781 tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca
      841 tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa
      901 ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt
      961 tataataaat cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat
     1021 ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata
     1081 atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta
     1141 gtactttctt tagcagaaca ggatgaacga acgtattcga attgtgaccc ttgaggggtc
     1201 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     1261 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     1321 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     1381 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     1441 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     1501 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     1561 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     1621 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     1681 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     1741 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     1801 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     1861 ggcggtatcc ggtgaggtgg cggataggca taatgaagcc tttccgccac ctcaccggat
//
LOCUS       DAFFQY010000244         1385 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.168, whole genome shotgun sequence.
ACCESSION   DAFFQY010000244 DAFFQY010000000
VERSION     DAFFQY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.168"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            <1..1361
                     /gene="tet(L)"
                     /locus_tag="LRB02_002704"
     CDS             <1..1361
                     /gene="tet(L)"
                     /locus_tag="LRB02_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBL4660827.1"
                     /translation="CNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFNKPPAS
                     TNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIM
                     ARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI
                     HWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSIS
                     FLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYM
                     MKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEG
                     TGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQR
                     DF"
BASE COUNT          385 a          212 c          279 g          509 t
ORIGIN      
        1 aatgtaacag ggttaaatac aaccaaattt taatttggct ttgcgtttta tcttttttta
       61 gcgtattaaa tgaaatggtt ttgaacgtct cattacctga tattgcaaat gattttaata
      121 aaccacctgc gagtacaaac tgggtgaaca cagcctttat gttaaccttt tccattggaa
      181 cagctgtata tggaaagcta tctgatcaat taggcatcaa aaggttactc ctatttggaa
      241 ttataataaa ttgtttcggg tcggtaattg ggtttgttgg ccattctttc ttttccttac
      301 ttattatggc tcgttttatt caaggggctg gtgcagctgc atttccagca ctcgtaatgg
      361 ttgtagttgc gcgctatatt ccaaaggaaa ataggggtaa agcatttggt cttattggat
      421 cgatagtagc catgggagaa ggagtcggtc cagcgattgg tggaatgata gcccattata
      481 ttcattggtc ctatcttcta ctcattccta tgataacaat tatcactgtt ccgtttctta
      541 tgaaattatt aaagaaagaa gtaaggataa aaggtcattt tgatatcaaa ggaattatac
      601 taatgtctgt aggcattgta ttttttatgt tgtttacaac atcatatagc atttcttttc
      661 ttatcgttag cgtgctgtca ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc
      721 cttttgttga tcccggatta gggaaaaata taccttttat gattggagtt ctttgtgggg
      781 gaattatatt tggaacagta gcagggtttg tctctatggt tccttatatg atgaaagatg
      841 ttcaccagct aagtactgcc gaaatcggaa gtgtaattat tttccctgga acaatgagtg
      901 tcattatttt cggctacatt ggtgggatac ttgttgatag aagaggtcct ttatacgtgt
      961 taaacatcgg agttacattt ctttctgtta gctttttaac tgcttccttt cttttagaaa
     1021 caacatcatg gttcatgaca attataatcg tatttgtttt aggtgggctt tcgttcacca
     1081 aaacagttat atcaacaatt gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa
     1141 tgagtttgct taactttacc agctttttat cagagggaac aggtattgca attgtaggtg
     1201 gtttattatc cataccctta cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa
     1261 cttatctgta tagtaatttg ttattacttt tttcaggaat cattgtcatt agttggctgg
     1321 ttaccttgaa tgtatataaa cattctcaaa gggatttcta aatcgttaag ggatcaactt
     1381 tggga
//
LOCUS       DAFFQY010000245         1328 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.171, whole genome shotgun sequence.
ACCESSION   DAFFQY010000245 DAFFQY010000000
VERSION     DAFFQY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.171"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            119..592
                     /gene="dfrF"
                     /locus_tag="LRB02_002705"
     CDS             119..592
                     /gene="dfrF"
                     /locus_tag="LRB02_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL4660828.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            719..>1328
                     /locus_tag="LRB02_002706"
     CDS             719..>1328
                     /locus_tag="LRB02_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL4660829.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWI"
BASE COUNT          498 a          143 c          280 g          407 t
ORIGIN      
        1 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
       61 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
      121 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
      181 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
      241 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
      301 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
      361 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
      421 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
      481 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
      541 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
      601 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
      661 taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag gattgaatat
      721 gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa agaaagatac
      781 ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac aaaccttagt
      841 atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca taaacgctga
      901 tggttatcat agtaagacga ttgcttattc tatttggcat attttccgca ttgaagatat
      961 agttgtgcat acagtgataa atgaagatga acaagtattt tttgcaggca attatcaaga
     1021 gcgtatcaat tcacctatca ttacaacagg aaatgaactc atgaaacagc agattgcaga
     1081 cttttcaaaa cagcttaatc tggaagaatt atatttatat attttcgaag tatgggaaag
     1141 cactgagaaa atgcttgaac ggttatctta tgatgaattg aaaagaaaaa taccgaaaga
     1201 aagaaaagga tacttagaat cgttgaatgt agtaaacgac aatgaaaaag caatttggct
     1261 gattgattat tggtgtaata aagatatttg tggactaatt caaatgccgt tttcaagaca
     1321 ttggatta
//
LOCUS       DAFFQY010000246          940 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.186, whole genome shotgun sequence.
ACCESSION   DAFFQY010000246 DAFFQY010000000
VERSION     DAFFQY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.186"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            118..912
                     /locus_tag="LRB02_002707"
                     /note="aph(3')-IIIa"
     CDS             118..912
                     /locus_tag="LRB02_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL4660830.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          292 a          155 c          247 g          246 t
ORIGIN      
        1 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
       61 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
      121 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
      181 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
      241 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
      301 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
      361 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
      421 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
      481 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
      541 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
      601 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
      661 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
      721 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
      781 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
      841 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
      901 gaattgtttt agtacctaga tttagatgtc taaaaagctt
//
LOCUS       DAFFQY010000247          850 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.188, whole genome shotgun sequence.
ACCESSION   DAFFQY010000247 DAFFQY010000000
VERSION     DAFFQY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.188"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            6..743
                     /gene="erm(B)"
                     /locus_tag="LRB02_002708"
     CDS             6..743
                     /gene="erm(B)"
                     /locus_tag="LRB02_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL4660831.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            688..>850
                     /locus_tag="LRB02_002709"
     CDS             688..>850
                     /locus_tag="LRB02_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBL4660832.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFFKFGKLHVTKGNGDKLLDIL
                     LTASKKLKRS"
BASE COUNT          318 a          148 c          135 g          249 t
ORIGIN      
        1 attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca
       61 accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag
      121 gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat
      181 tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt cgtgtcactt
      241 taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg
      301 ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccatg
      361 cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc
      421 gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag
      481 cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata
      541 ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca
      601 atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca
      661 aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc
      721 tattatttaa cgggaggaaa taattctatg agtcgctttt ttaaatttgg aaagttacac
      781 gttactaaag ggaatggaga taaattatta gatatactac tgacagcttc caagaagcta
      841 aagaggtccc
//
LOCUS       DAFFQY010000248          675 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.198, whole genome shotgun sequence.
ACCESSION   DAFFQY010000248 DAFFQY010000000
VERSION     DAFFQY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.198"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            118..660
                     /gene="sat4"
                     /locus_tag="LRB02_002710"
     CDS             118..660
                     /gene="sat4"
                     /locus_tag="LRB02_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL4660833.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          250 a          109 c          151 g          165 t
ORIGIN      
        1 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
       61 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
      121 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
      181 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
      241 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
      301 gattgccttg aatatattga caatactgat aagataatat atctttacta ccaagacgat
      361 aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat
      421 atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct
      481 atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac
      541 cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg
      601 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
      661 aatgcaagga acagt
//
LOCUS       DAFFQY010000249          655 bp    DNA     linear   BCT 29-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate PR05995_1
            SAMN20572371-rid13348313.guided.199, whole genome shotgun sequence.
ACCESSION   DAFFQY010000249 DAFFQY010000000
VERSION     DAFFQY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572371
            Sequence Read Archive: SRR16917162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176885.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176885.1
            Genome Coverage       :: 302x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:02:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,651
            Genes (coding)                    :: 2,583
            CDSs (with protein)               :: 2,583
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 45 of 68
            Pseudo Genes (incomplete)         :: 14 of 68
            Pseudo Genes (internal stop)      :: 18 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572371-rid13348313.guided.199"
                     /isolate="PR05995_1"
                     /isolation_source="Perirectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2016-07"
                     /collected_by="University of Michigan"
     gene            118..>655
                     /gene="sat4"
                     /locus_tag="LRB02_002711"
     CDS             118..>655
                     /gene="sat4"
                     /locus_tag="LRB02_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL4660834.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENSFFKVVFKVGV"
BASE COUNT          240 a          108 c          146 g          161 t
ORIGIN      
        1 tccggagtat gataggaata taacaaaata taccagggac atgtataaaa aatacactgg
       61 taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg
      121 attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag
      181 gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc
      241 tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca
      301 gattgccttg aatatattga caatactgat aagataatat atctttacta ccaagacgat
      361 aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat
      421 atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct
      481 atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac
      541 cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg
      601 ttatacgcca actttgaaaa cagctttttc aaagttgttt tcaaagttgg cgtat
//
